BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0042.2
(173 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g54770 T22H22.18 118 2e-27
At2g20370 unknown protein 34 0.045
At1g76810 translation initiation factor IF-2 like protein 33 0.059
At1g76720 putative translation initiation factor IF-2 33 0.10
At1g76820 putative translation initiation factor IF-2 32 0.13
At1g22610 Highly similar to phosphoribosylanthranilate transferase 32 0.17
At3g04810 kinase like protein 31 0.38
At5g64910 unknown protein 30 0.50
At2g18193 AAA-type ATPase like protein 30 0.50
At5g12400 putative protein 30 0.65
At2g45380 unknown protein 30 0.65
At5g06250 putative protein 30 0.85
At1g74860 unknown protein 29 1.5
At5g24880 glutamic acid-rich protein 28 1.9
At2g22100 putative RNA-binding protein 28 1.9
At3g03710 putative polynucleotide phosphorylase 28 2.5
At2g42400 unknown protein 28 2.5
At1g17450 hypothetical protein 28 2.5
At5g14440 putative protein 28 3.2
At3g50170 putative protein 28 3.2
>At1g54770 T22H22.18
Length = 189
Score = 118 bits (295), Expect = 2e-27
Identities = 72/171 (42%), Positives = 90/171 (52%), Gaps = 42/171 (24%)
Query: 36 GSHSTSQAESSTITLWKHNSELVHALFVPPNEPTKLNKLLREQVKDTTGRIWFDMPAQTI 95
GS + AESS SELV L +PPN+P K+NK++R+Q+KDTTG WFDMPA T+
Sbjct: 27 GSTRSKTAESS-------KSELVDGLCLPPNDPRKINKMIRKQLKDTTGSNWFDMPAPTM 79
Query: 96 NPELQKDETSLQLRAAIDPKRHYKKGNSKSKALPKYFQ---------------------- 133
PEL++D L+LR +DP HYKK S+SK KYFQ
Sbjct: 80 TPELKRDLQLLKLRTVMDPALHYKKSVSRSKLAEKYFQIGTVIEPAEEFYGRLTKKNRKA 139
Query: 134 ------------ASSGARKVREIEEQNQPAGNEKWKVKGGNSRKRAKERRN 172
A RKVREIEE+++ N+KW K GN K K RRN
Sbjct: 140 TLADELVSDPKTALYRKRKVREIEEKSRAVTNKKWN-KKGNQSKNTKPRRN 189
>At2g20370 unknown protein
Length = 600
Score = 33.9 bits (76), Expect = 0.045
Identities = 24/89 (26%), Positives = 39/89 (42%), Gaps = 3/89 (3%)
Query: 68 PTKLNKLLREQVKDTTGRIWFDMPAQTINPELQKDETSLQLRAAIDPKRHYKKGNSKSKA 127
P K K++RE V + R+ + P + E QKD + ++A ID +K + +
Sbjct: 474 PAKQVKIMRENVINLIPRLIYADPRSEL--ETQKDAFDVSVQAVIDKVTRLRKNMIEGRT 531
Query: 128 LPKYFQASSGARKVREIEEQNQPAGNEKW 156
YF + K +EE + AG W
Sbjct: 532 EYDYF-VEENSWKYALLEEGQREAGGHVW 559
>At1g76810 translation initiation factor IF-2 like protein
Length = 1280
Score = 33.5 bits (75), Expect = 0.059
Identities = 21/80 (26%), Positives = 39/80 (48%), Gaps = 6/80 (7%)
Query: 92 AQTINPELQKDETSLQLRAAIDPKRHYKKGNSKSKALPKYFQASSGARKVREIEEQNQPA 151
A T + E+++++ + + PK+ KG + K +PK+ R+++E + Q A
Sbjct: 373 AATSSVEVKEEKQEESVTEPLQPKKKDAKGKAAEKKIPKH------VREMQEALARRQEA 426
Query: 152 GNEKWKVKGGNSRKRAKERR 171
K K + RK +ERR
Sbjct: 427 EERKKKEEEEKLRKEEEERR 446
>At1g76720 putative translation initiation factor IF-2
Length = 1224
Score = 32.7 bits (73), Expect = 0.10
Identities = 21/80 (26%), Positives = 38/80 (47%), Gaps = 6/80 (7%)
Query: 92 AQTINPELQKDETSLQLRAAIDPKRHYKKGNSKSKALPKYFQASSGARKVREIEEQNQPA 151
A T + E ++++ + + PK+ KG + K +PK+ R+++E + Q A
Sbjct: 349 AATSSVEAKEEKQEESVTEPLQPKKKDAKGKAAEKKIPKH------VREMQEALARRQEA 402
Query: 152 GNEKWKVKGGNSRKRAKERR 171
K K + RK +ERR
Sbjct: 403 EERKKKEEEEKLRKEEEERR 422
>At1g76820 putative translation initiation factor IF-2
Length = 1146
Score = 32.3 bits (72), Expect = 0.13
Identities = 20/80 (25%), Positives = 38/80 (47%), Gaps = 6/80 (7%)
Query: 92 AQTINPELQKDETSLQLRAAIDPKRHYKKGNSKSKALPKYFQASSGARKVREIEEQNQPA 151
A T + E ++++ + + P++ KG + K +PK+ R+++E + Q A
Sbjct: 259 AATSSVEAKEEKQEESVTEPLQPRKKDAKGKAAEKKIPKH------VREIQEALARRQEA 312
Query: 152 GNEKWKVKGGNSRKRAKERR 171
K K + RK +ERR
Sbjct: 313 KERKKKEEEEKLRKEEEERR 332
>At1g22610 Highly similar to phosphoribosylanthranilate transferase
Length = 1029
Score = 32.0 bits (71), Expect = 0.17
Identities = 28/112 (25%), Positives = 47/112 (41%), Gaps = 12/112 (10%)
Query: 16 PTKLRWSQRRHFCAVLCRDDGSHSTSQAESSTITLWKHNSELVHALFVPPNEPTKLNKLL 75
PTK + + R F ++ G+H+ + + S+ A PPN+P + +
Sbjct: 186 PTKKKEKESRTFHSI-----GAHAGGGGGAPPM------SQAKQAYPPPPNQPEFRSDFM 234
Query: 76 REQVKDTTGRIWFDMPAQTINPELQKDETSLQLRAAIDPKRHYKKGNSKSKA 127
R TG + P + NPE Q ETS L A + +Y+ K+ +
Sbjct: 235 RAP-GPPTGAVMQMQPPRQQNPEFQLIETSPPLAARMRQSYYYRSSGDKTSS 285
>At3g04810 kinase like protein
Length = 606
Score = 30.8 bits (68), Expect = 0.38
Identities = 19/60 (31%), Positives = 28/60 (46%), Gaps = 3/60 (5%)
Query: 96 NPELQKDETSLQLRAAIDP---KRHYKKGNSKSKALPKYFQASSGARKVREIEEQNQPAG 152
NPEL+ L + + P K H K + S LP + S AR+ E++ +PAG
Sbjct: 242 NPELRPSAAELLRQPLLQPYIQKIHLKVNDPGSNVLPAQWPESESARRNSFPEQRRRPAG 301
>At5g64910 unknown protein
Length = 487
Score = 30.4 bits (67), Expect = 0.50
Identities = 29/143 (20%), Positives = 61/143 (42%), Gaps = 18/143 (12%)
Query: 36 GSHSTSQAESSTITLWKHNSELVHALFVPPNEPTKLNKLLREQVKDTTGRIWFDMPAQTI 95
G T + S++ K++ +V A +PT ++ ++T ++ P +
Sbjct: 17 GGRKTGASSSAS----KNDDAVVEATTTQETQPT-------QETEETEDKVESPAPEEEG 65
Query: 96 NPELQKDETSLQLRAAIDPKRHYKKGNSKSKALPKYFQASSGARKVREIEEQ---NQPAG 152
E + +E + A ++ K + GN + K + AR+ +E EE+ +
Sbjct: 66 KNEEEANENQEEEAAKVESKAAEEGGNEEEAKEDKEEEKEEAAREDKEEEEEAVKPDESA 125
Query: 153 NEKWKVKGGNSR----KRAKERR 171
++K + KG +S +R K +R
Sbjct: 126 SQKEEAKGASSSEPQLRRGKRKR 148
>At2g18193 AAA-type ATPase like protein
Length = 495
Score = 30.4 bits (67), Expect = 0.50
Identities = 23/78 (29%), Positives = 34/78 (43%), Gaps = 6/78 (7%)
Query: 91 PAQTINPELQKDETSLQLRAAIDPKRHYKKGNSKSKALPKYFQASSGARKVREIEEQNQP 150
PA+ +Q D+T + LR I K SK+K + S + + E+QN
Sbjct: 414 PAELAEELMQDDDTDVVLRGVISFVEKRKVERSKTK------KEVSICKATDDDEKQNGS 467
Query: 151 AGNEKWKVKGGNSRKRAK 168
G K K KGG + + K
Sbjct: 468 LGCVKKKKKGGKQKGKGK 485
>At5g12400 putative protein
Length = 1595
Score = 30.0 bits (66), Expect = 0.65
Identities = 23/74 (31%), Positives = 34/74 (45%), Gaps = 4/74 (5%)
Query: 96 NPELQKDETSLQLRAAIDPKRHYKKGNSKSKALPKYFQASSGARKVREIEEQNQPAGNEK 155
+P L+K +TS+ R AI K H K SK P +R +E P G++K
Sbjct: 1406 SPRLKKTKTSMAERIAIRLKNHKKVVASK----PLRRSGRQLKHVIRLQDESKVPEGSKK 1461
Query: 156 WKVKGGNSRKRAKE 169
K++ R R K+
Sbjct: 1462 RKLETKRGRGRPKK 1475
>At2g45380 unknown protein
Length = 162
Score = 30.0 bits (66), Expect = 0.65
Identities = 23/87 (26%), Positives = 39/87 (44%), Gaps = 11/87 (12%)
Query: 71 LNKLLREQVKDTTGR-----------IWFDMPAQTINPELQKDETSLQLRAAIDPKRHYK 119
L+K+L ++V + TG + FD I EL ET+ K K
Sbjct: 3 LDKVLEDEVVEDTGESQVFNLLNPEFVDFDTCLDPITNELPTSETTKPAGELRREKTPAK 62
Query: 120 KGNSKSKALPKYFQASSGARKVREIEE 146
KGNS S ++ F ++S A +++++
Sbjct: 63 KGNSNSSSVDHRFTSASRAFTQKKLDD 89
>At5g06250 putative protein
Length = 282
Score = 29.6 bits (65), Expect = 0.85
Identities = 18/60 (30%), Positives = 32/60 (53%), Gaps = 14/60 (23%)
Query: 19 LRWSQRRHFCAVLCRDDGSHSTSQAESSTITLWKHNSELVHALF---VPPNEPTKLNKLL 75
L W Q++H H+T +E++T W H+ +L +LF + P++ KLN+L+
Sbjct: 14 LLWQQQQH----------RHTTDTSETTTTATWLHD-DLKESLFEKSLTPSDVGKLNRLV 62
>At1g74860 unknown protein
Length = 369
Score = 28.9 bits (63), Expect = 1.5
Identities = 19/92 (20%), Positives = 42/92 (45%), Gaps = 2/92 (2%)
Query: 1 MRKQRTERIRLRSENPTKLRWSQRRHFCAVLCRDDGSHSTSQAESSTITLWKHNSELVHA 60
++K + L+ + L W+ R +V+ SH ++A I + ++ S + +
Sbjct: 107 LKKLTDGQKHLKKNHEENLCWNNRDAAVSVMPEVSCSHENTEAVGFCIPVEQNGSVVANG 166
Query: 61 LFVPPNEPTKLNKLLREQVKDTTGRIWFDMPA 92
+ VP T++N + R KD + + +P+
Sbjct: 167 IKVP--SLTRINSINRCSYKDNKDQCHYTLPS 196
>At5g24880 glutamic acid-rich protein
Length = 443
Score = 28.5 bits (62), Expect = 1.9
Identities = 16/77 (20%), Positives = 35/77 (44%)
Query: 96 NPELQKDETSLQLRAAIDPKRHYKKGNSKSKALPKYFQASSGARKVREIEEQNQPAGNEK 155
N +K +T + +++ K+ K + + + KV+E +++ + EK
Sbjct: 312 NETPEKVDTESKEVESVEETTQEKEEEVKEEGKERVEEEEKEKEKVKEDDQKEKVEEEEK 371
Query: 156 WKVKGGNSRKRAKERRN 172
KVKG +++ KE +
Sbjct: 372 EKVKGDEEKEKVKEEES 388
>At2g22100 putative RNA-binding protein
Length = 382
Score = 28.5 bits (62), Expect = 1.9
Identities = 17/58 (29%), Positives = 28/58 (47%)
Query: 111 AIDPKRHYKKGNSKSKALPKYFQASSGARKVREIEEQNQPAGNEKWKVKGGNSRKRAK 168
A+ K+ KK SKSK K ++ ++K E+EE+ + K S+K+ K
Sbjct: 30 AVREKKISKKHKSKSKEEEKPEKSKKKSKKYEEVEEEEKSPSPSPSPKKSKESKKKHK 87
>At3g03710 putative polynucleotide phosphorylase
Length = 948
Score = 28.1 bits (61), Expect = 2.5
Identities = 25/117 (21%), Positives = 47/117 (39%), Gaps = 6/117 (5%)
Query: 53 HNSELVHALFVPPNEPTKLNKLLREQVKDTTGRIWFDMPAQTINPELQKDETSLQLRAAI 112
H SEL P + K+ + ++ + + + + + PE + D+ S + + A
Sbjct: 817 HISELSAEWLAKPEDAYKVGDRIDVKLIEVNEKGQLRLSVRALLPESETDKDSQKQQPAG 876
Query: 113 DP------KRHYKKGNSKSKALPKYFQASSGARKVREIEEQNQPAGNEKWKVKGGNS 163
D +R Y +SK +A + SSG V + ++ + G K NS
Sbjct: 877 DSTKDKSSQRKYVNTSSKDRAAAGASKVSSGDELVLKKKDVRRATGGSSDKTMNSNS 933
>At2g42400 unknown protein
Length = 450
Score = 28.1 bits (61), Expect = 2.5
Identities = 20/75 (26%), Positives = 32/75 (42%), Gaps = 10/75 (13%)
Query: 92 AQTINPELQKDETSLQLRAAIDPKRHYKKGNSKSKALPKYFQASSGARKVREIEEQN--- 148
A+ + EL + ET + P+R Y GN K ++LP Y + + ++EQ
Sbjct: 312 AELFHLELVEGETIREWLFFDKPRRAYDSGNRKQRSLPDYSGRGWHESRKQLMKEQEGQK 371
Query: 149 -------QPAGNEKW 156
QP G +W
Sbjct: 372 RSYYMDPQPPGPFEW 386
>At1g17450 hypothetical protein
Length = 1783
Score = 28.1 bits (61), Expect = 2.5
Identities = 14/36 (38%), Positives = 22/36 (60%)
Query: 77 EQVKDTTGRIWFDMPAQTINPELQKDETSLQLRAAI 112
+QVKDT+ R QT+ P +KD S+Q+R ++
Sbjct: 1228 DQVKDTSRRSGHYRLHQTVRPLDEKDNDSIQVRKSL 1263
>At5g14440 putative protein
Length = 291
Score = 27.7 bits (60), Expect = 3.2
Identities = 30/128 (23%), Positives = 42/128 (32%), Gaps = 28/128 (21%)
Query: 65 PNEPTKLN-KLLREQVKDTTGRIWFDMPAQTI-----NPELQKDETSLQLRAAIDP---- 114
PN +KL KL + V T IW + + E +K+ S+ P
Sbjct: 77 PNARSKLKCKLTGDTVNKTEEHIWKHITGRRFLNRLEEKEREKESGSIPAEGGETPAKEN 136
Query: 115 -------KRHYKKGNSKSKALPKYFQASSGARKVREIEEQN-----------QPAGNEKW 156
K+ KK N K K + +G EIE +N P E+W
Sbjct: 137 GVEDEDKKKKKKKNNKKKKNKKSVEKKKNGEDVADEIEHENDEAVEEELEFWMPPDGERW 196
Query: 157 KVKGGNSR 164
G R
Sbjct: 197 DFDDGRDR 204
>At3g50170 putative protein
Length = 541
Score = 27.7 bits (60), Expect = 3.2
Identities = 21/98 (21%), Positives = 41/98 (41%), Gaps = 5/98 (5%)
Query: 73 KLLREQVKDTTGRIWFDMPAQTINPELQKDETSLQLRAAIDPKRHYKKGNSKSKALPKYF 132
+++ E+ ++TTG W + + D+T++ + I HY + N K YF
Sbjct: 76 EVVEERPEETTGDSWVISIRDKLEQADRDDDTTIWGKLCIYRVPHYLQENDKK----SYF 131
Query: 133 QASSGARKVREIEEQNQPAGNEKWKVKGGNSRKRAKER 170
+ +++ +P KW+ KR K+R
Sbjct: 132 PQTVSLGPYHHGKKRLRPMERHKWRAL-NKVLKRLKQR 168
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.313 0.128 0.376
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,062,869
Number of Sequences: 26719
Number of extensions: 158918
Number of successful extensions: 399
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 23
Number of HSP's that attempted gapping in prelim test: 381
Number of HSP's gapped (non-prelim): 40
length of query: 173
length of database: 11,318,596
effective HSP length: 92
effective length of query: 81
effective length of database: 8,860,448
effective search space: 717696288
effective search space used: 717696288
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0042.2