BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0034b.2
(494 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g33310 putative protein 37 0.019
At5g53870 predicted GPI-anchored protein 36 0.056
At5g32590 putative protein 35 0.073
At3g32940 hypothetical protein 33 0.28
At4g20730 putative protein 33 0.36
At5g13260 unknown protein 32 0.80
At3g14020 putative transcription factor 32 0.80
At4g16740 limonene cyclase like protein 31 1.4
At4g20070 hyuC-like protein 31 1.8
At5g38190 putative protein 30 3.1
At3g14460 disease resistance protein, putative 30 3.1
At1g32010 hypothetical protein 30 3.1
At5g38640 Unknown protein (At5g38640; MBB18.19) 30 4.0
At5g08750 unknown protein 30 4.0
At3g05680 unknown protein 30 4.0
At5g38560 putative protein 29 6.8
At2g38500 unknown protein 29 6.8
At5g58540 unknown protein 28 8.9
At5g10550 bromodomain protein - like 28 8.9
At5g08230 putative protein 28 8.9
>At5g33310 putative protein
Length = 1137
Score = 37.4 bits (85), Expect = 0.019
Identities = 40/158 (25%), Positives = 59/158 (37%), Gaps = 14/158 (8%)
Query: 292 PGDRPWLHKPDGDVKRSHFFFAYEYMFSELGIRLPFSPFVQTVLRDINAAPCQLHPNAWA 351
P + P H+P FF YE F G+ P + L + A QL PN
Sbjct: 91 PTESPEDHRPG-------FFCVYEIYFKGCGLTFPLPEALVRYLAALEIALPQLTPNLLR 143
Query: 352 FIRCFEILSAAVGIAPSPTSFFYLYDVDPKSIKNKGWISLKARAGRKCLHPHKSNAKSSF 411
I C ++A G L V S K G+ S A + L + N ++
Sbjct: 144 TILCIITIAAEAGFVIRVPELNELLSVRSSS-KKTGYFSAYPNANQN-LISNLLNKDENW 201
Query: 412 ARKYFRVAVHPAYPEAFTLRDGTALFPLYWTEKPNGIT 449
+F V PA ++ + + + P W+ KP IT
Sbjct: 202 HHPWFLVKKTPA-----SIGNLSDMLPSKWSAKPPFIT 234
>At5g53870 predicted GPI-anchored protein
Length = 370
Score = 35.8 bits (81), Expect = 0.056
Identities = 29/92 (31%), Positives = 41/92 (44%), Gaps = 3/92 (3%)
Query: 59 APRDASVTSISLFLRNRHFPFHNRPIVRSNSPLHA-THLPKTIMTSNLPHA--SNTRHPS 115
+P+ S S S H P H+ S+SP HA +H P + + HA + H
Sbjct: 210 SPKSPSPVSHSPSHSPAHTPSHSPAHTPSHSPAHAPSHSPAHAPSHSPAHAPSHSPAHSP 269
Query: 116 SFSPSPIKTLLPHSHPFLRPLFTSLIAYQTPS 147
S SP+ K+ P S P P S + Q+PS
Sbjct: 270 SHSPATPKSPSPSSSPAQSPATPSPMTPQSPS 301
Score = 33.9 bits (76), Expect = 0.21
Identities = 35/134 (26%), Positives = 49/134 (36%), Gaps = 3/134 (2%)
Query: 86 RSNSPLHATHLPKTIMTSNLPHASNTRHPSSFSPSPIKTLLPHSHPFLRPLFTSLIAYQT 145
+S+SP+ +H P + H+ T PS SPSP+ HS P P +
Sbjct: 183 KSSSPI--SHTPALSPSHATSHSPATPSPSPKSPSPVSHSPSHS-PAHTPSHSPAHTPSH 239
Query: 146 PSAHFRSGAVAYHPFRYPLFTSYSGESHCPYLCITHILIFSFLSLHCLLKMASNPTSPNH 205
AH S + A+ P P +H P S S +P +P
Sbjct: 240 SPAHAPSHSPAHAPSHSPAHAPSHSPAHSPSHSPATPKSPSPSSSPAQSPATPSPMTPQS 299
Query: 206 SSSSSGSDPDATAA 219
S S PD +AA
Sbjct: 300 PSPVSSPSPDQSAA 313
>At5g32590 putative protein
Length = 871
Score = 35.4 bits (80), Expect = 0.073
Identities = 59/243 (24%), Positives = 80/243 (32%), Gaps = 39/243 (16%)
Query: 227 PEIPPYRLKTYATLPLPVQI----------APTHEAIDQPSIFQN--------------S 262
P I P L TLPLP ++ P + D N S
Sbjct: 86 PTISPPSLSNGETLPLPPRLPRRFRHHHWLTPESPSFDDVGSSNNLCTRSNDIAGPHYQS 145
Query: 263 DLIVQFVNSLGGLSNDDEFSAKLRIWICSPGDRPWLHKPDGDVKRSHFFFAYEYMFSELG 322
+Q +N LG L K + P + P H+P FF YE F G
Sbjct: 146 TCTLQSLNRLGELCRIPAEVMK-DLQTPEPTESPEDHRPG-------FFCVYEIYFKGCG 197
Query: 323 IRLPFSPFVQTVLRDINAAPCQLHPNAWAFIRCFEILSAAVGIAPSPTSFFYLYDVDPKS 382
+ P + L + A QL PN I ++A G L +V +S
Sbjct: 198 LTFPLPEALVRYLAALEIALPQLTPNLLWTILGIITIAAEAGYVIGVPELNELLNV-RRS 256
Query: 383 IKNKGWISLKARAGRKCLHPHKSNAKSSFARKYFRVAVHPAYPEAFTLRDGTALFPLYWT 442
K G+ S A R L H N + +F V P ++ + L P WT
Sbjct: 257 SKKVGYFSAYPNANRN-LISHLPNKDENEHHPWFLVKKSPT-----SIGNLPDLLPSKWT 310
Query: 443 EKP 445
KP
Sbjct: 311 TKP 313
>At3g32940 hypothetical protein
Length = 590
Score = 33.5 bits (75), Expect = 0.28
Identities = 26/95 (27%), Positives = 40/95 (41%), Gaps = 12/95 (12%)
Query: 55 PHVAAPRDASVTSISLFLRNRHFPFH-NRPIVRSNSPLHATHLPKT------IMTSNLPH 107
PH+ P AS F R++HF ++P+ ++P H +M N P
Sbjct: 484 PHL--PFQASQHFGQNFTRSQHFDRQMDQPLSHPSAPFHGNARSNVGPPLSQMMPRNFPG 541
Query: 108 ASNTRHPSSFSPSPI---KTLLPHSHPFLRPLFTS 139
A +H + F P P+ L+P P +R F S
Sbjct: 542 AQFPQHSAHFPPRPVFHHDNLIPRGQPQIRQRFNS 576
>At4g20730 putative protein
Length = 824
Score = 33.1 bits (74), Expect = 0.36
Identities = 59/276 (21%), Positives = 99/276 (35%), Gaps = 33/276 (11%)
Query: 195 KMASNPTSPNHSSSSSG-------SDPDATAAGGLRLEAPEIPPYRLK----------TY 237
++ NP++ SS S+ DPD +GG L APE P L Y
Sbjct: 26 RLTGNPSNEAQSSRSNRIEIPTLLDDPDREGSGG-HLAAPEDPVDFLAQFDPLIDLEDAY 84
Query: 238 ATLPLPVQIAPTHEAIDQPSIFQNSDLIVQFVNSLGGLSNDDEFSAKLRIW-ICSPGDRP 296
T P + + + + + D +V + + + ++ +C GDRP
Sbjct: 85 NTQPQYTEAEVNARLLHEDHVEFDLDPVVSWNTEITAKKTLSTTESVAEVFALCGSGDRP 144
Query: 297 WLHKPDGDVKR-----SHFFFAYEYMFSELGIRLPFSPFVQTVLRDINAAPCQLHPNAWA 351
+ G+ KR + YE F + + P + + L + A QL P A
Sbjct: 145 LTYMIPGENKRPWNPPRGYVCMYEAYFRQCHLWFPIPSLIISFLNRRHMAFSQLTPAAIC 204
Query: 352 FIRCFEILSAAVGIAPSPTSFFYLYDVDPKSIKNKGWISLKARAGRKCLHPHKSNAKSSF 411
A G + F + + K+I++ G+ + R L K + ++
Sbjct: 205 NFVAALTFGAEEGYLVNVRCFEEMTTL--KAIRSPGYWVVNNRPKHNFLPGPKVSNFKNW 262
Query: 412 ARKYFRVAVH-PAYPEAFTLRDGTALFPLYWTEKPN 446
YF V V +Y F+ R WTE P+
Sbjct: 263 EEYYFYVRVDLESYERPFSGR------KRMWTEFPD 292
>At5g13260 unknown protein
Length = 537
Score = 32.0 bits (71), Expect = 0.80
Identities = 15/45 (33%), Positives = 22/45 (48%)
Query: 209 SSGSDPDATAAGGLRLEAPEIPPYRLKTYATLPLPVQIAPTHEAI 253
S S P + A +R P +PP +L+ T PLPV T + +
Sbjct: 128 SRSSSPANSPAVSVRASQPPVPPSKLRNQTTNPLPVATPKTEKRV 172
>At3g14020 putative transcription factor
Length = 308
Score = 32.0 bits (71), Expect = 0.80
Identities = 20/64 (31%), Positives = 29/64 (45%), Gaps = 2/64 (3%)
Query: 83 PIVRSNSPLHATHLPKTIMTSNLPHASN--TRHPSSFSPSPIKTLLPHSHPFLRPLFTSL 140
PI ++ PLH P + P+AS +HP P + LPH+ P P+F +
Sbjct: 118 PIEAASWPLHGNVTPHFNGFLSFPYASQHTVQHPQIRGLVPSRMPLPHNIPENEPIFVNA 177
Query: 141 IAYQ 144
YQ
Sbjct: 178 KQYQ 181
>At4g16740 limonene cyclase like protein
Length = 1024
Score = 31.2 bits (69), Expect = 1.4
Identities = 27/109 (24%), Positives = 40/109 (35%), Gaps = 23/109 (21%)
Query: 194 LKMASNPTSPNHSSSSSGSDPDATAAGGLRLEAPEIPPYRLKTYATLPLPVQIAPTHEAI 253
L + P +P H SSS A +PP + TYA L E
Sbjct: 618 LILTDPPPAPRHVLSSS------------ERRALSLPPKKALTYAKL----------EIQ 655
Query: 254 DQPSIFQNSDLIVQFVNSLGGLSNDDEFSAK-LRIWICSPGDRPWLHKP 301
P + ++DL +L E L+++I GD+P H+P
Sbjct: 656 RAPEVINDTDLFAPLFRNLSVFKRSYELMELILKVYIYPDGDKPIFHEP 704
>At4g20070 hyuC-like protein
Length = 525
Score = 30.8 bits (68), Expect = 1.8
Identities = 24/67 (35%), Positives = 34/67 (49%), Gaps = 3/67 (4%)
Query: 149 HFRSGAVAYHPFRYPLF-TSYSGESHC--PYLCITHILIFSFLSLHCLLKMASNPTSPNH 205
H S + HPF ++ TS+ H P L + L+FS LS L +S+ +S +
Sbjct: 5 HPSSSSSRSHPFLSHVYHTSFHHHHHHNHPSLVLFWCLVFSLLSPLALSSSSSSSSSSSD 64
Query: 206 SSSSSGS 212
SSSSS S
Sbjct: 65 SSSSSSS 71
>At5g38190 putative protein
Length = 969
Score = 30.0 bits (66), Expect = 3.1
Identities = 17/56 (30%), Positives = 25/56 (44%)
Query: 310 FFFAYEYMFSELGIRLPFSPFVQTVLRDINAAPCQLHPNAWAFIRCFEILSAAVGI 365
F +E F+E G+R P F+ R+ A QL + C ++L A GI
Sbjct: 161 FICLFEGFFTETGLRFPIPDFLMRFCRNRQIAISQLTVASIRTAACLQMLCARCGI 216
>At3g14460 disease resistance protein, putative
Length = 1424
Score = 30.0 bits (66), Expect = 3.1
Identities = 19/54 (35%), Positives = 26/54 (47%), Gaps = 1/54 (1%)
Query: 439 LYWTEKPNGITDPSEESLSANDKAFLNLLAPLPILDCTTVLEGADLSRTPKYLG 492
L WT K +G S +L+ + K L +L P P L T +E PK+LG
Sbjct: 727 LKWTVKGSGFVPGSFNALACDQKEVLRMLEPHPHLK-TFCIESYQGGAFPKWLG 779
>At1g32010 hypothetical protein
Length = 835
Score = 30.0 bits (66), Expect = 3.1
Identities = 17/56 (30%), Positives = 25/56 (44%)
Query: 310 FFFAYEYMFSELGIRLPFSPFVQTVLRDINAAPCQLHPNAWAFIRCFEILSAAVGI 365
F +E F+E G+R P F+ R+ A QL + C ++L A GI
Sbjct: 161 FICLFEGFFTETGLRFPIPDFLMRFCRNRQIAISQLTVASIRTAACLQMLCARCGI 216
>At5g38640 Unknown protein (At5g38640; MBB18.19)
Length = 642
Score = 29.6 bits (65), Expect = 4.0
Identities = 18/57 (31%), Positives = 26/57 (45%)
Query: 206 SSSSSGSDPDATAAGGLRLEAPEIPPYRLKTYATLPLPVQIAPTHEAIDQPSIFQNS 262
+++S+ S + G L IPP RL + T PL V +A + PS F S
Sbjct: 39 AATSASSSSSTISPSGNSLSPVMIPPPRLNSSDTFPLRVAVAGAAASAVSPSSFSYS 95
>At5g08750 unknown protein
Length = 363
Score = 29.6 bits (65), Expect = 4.0
Identities = 13/21 (61%), Positives = 16/21 (75%)
Query: 199 NPTSPNHSSSSSGSDPDATAA 219
NP N+++SSSG DPDA AA
Sbjct: 191 NPVVVNNNTSSSGIDPDAVAA 211
>At3g05680 unknown protein
Length = 2070
Score = 29.6 bits (65), Expect = 4.0
Identities = 30/153 (19%), Positives = 59/153 (37%), Gaps = 6/153 (3%)
Query: 119 PSPIKTLLPHSHPFLRPLFTSLIAYQTPSAHFRSGAVAYHPFRYPLFTSYSGESHCPYLC 178
P + ++PHS L + I++ T S+ + + P P ++S S P
Sbjct: 1767 PPSVSPVIPHSSDSLSNQSSPFISHGTQSSGGPTRLMPPLPSAIPQYSSNPYASLPPRTS 1826
Query: 179 ITHILIFSFLSLHCLLKMASNPTSPNHSSSSSGSDPDATAAGGLRLEAPEIPPYRLKTYA 238
++ + + S PT + S + S + + L +P + +
Sbjct: 1827 TVQSFGYNHAGVGTTEQQQSGPTIDHQSGNLSVTGMTSYPPPNL------MPSHNFSRPS 1880
Query: 239 TLPLPVQIAPTHEAIDQPSIFQNSDLIVQFVNS 271
+LP+P P+H+ D+P I Q +N+
Sbjct: 1881 SLPVPFYGNPSHQGGDKPQTMLLVPSIPQSLNT 1913
>At5g38560 putative protein
Length = 681
Score = 28.9 bits (63), Expect = 6.8
Identities = 17/59 (28%), Positives = 26/59 (43%), Gaps = 1/59 (1%)
Query: 78 PFHNRPIVRSNSPLHATHLPKTIMTSNLPHASNTRHPSSFSPSPIK-TLLPHSHPFLRP 135
P +P + +P T P TS P +SN PS+ +P P ++P P +P
Sbjct: 153 PSPPKPSPSTPTPTTTTSPPPPPATSASPPSSNPTDPSTLAPPPTPLPVVPREKPIAKP 211
>At2g38500 unknown protein
Length = 356
Score = 28.9 bits (63), Expect = 6.8
Identities = 14/34 (41%), Positives = 18/34 (52%)
Query: 151 RSGAVAYHPFRYPLFTSYSGESHCPYLCITHILI 184
R G + YHP Y TS+S E C L ++H I
Sbjct: 288 RQGEIIYHPDAYGSPTSFSVELKCMSLFLSHTSI 321
>At5g58540 unknown protein
Length = 484
Score = 28.5 bits (62), Expect = 8.9
Identities = 16/65 (24%), Positives = 28/65 (42%), Gaps = 4/65 (6%)
Query: 211 GSDPDATAAGGLRLEAPEIPPYRLKTYATLPLPVQIAPTHEAIDQPSIFQNSDLIVQFVN 270
G++ + G+ + PE PP LP+P+ AP+ P + S + V
Sbjct: 92 GNETPSPPRSGVPTQTPETPP----AITPLPVPLAPAPSPSPPVSPGTTKKSPKVYMIVG 147
Query: 271 SLGGL 275
+GG+
Sbjct: 148 IVGGV 152
>At5g10550 bromodomain protein - like
Length = 678
Score = 28.5 bits (62), Expect = 8.9
Identities = 15/33 (45%), Positives = 20/33 (60%)
Query: 198 SNPTSPNHSSSSSGSDPDATAAGGLRLEAPEIP 230
S+ +S + S SSSGSD DA + +EA E P
Sbjct: 624 SSSSSDSESGSSSGSDSDADSVQSPFVEAKEAP 656
>At5g08230 putative protein
Length = 1445
Score = 28.5 bits (62), Expect = 8.9
Identities = 15/52 (28%), Positives = 26/52 (49%), Gaps = 1/52 (1%)
Query: 72 LRNRHFPFHNRPIVRSNSPLHATHLPKTI-MTSNLPHASNTRHPSSFSPSPI 122
LR + F RP++R+ +P ++H P + + P S+ HP F P+
Sbjct: 1299 LRPINTSFLQRPMIRNLAPAPSSHFPLPCRIVQSEPQRSSFPHPYHFPSQPV 1350
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.322 0.138 0.434
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,167,159
Number of Sequences: 26719
Number of extensions: 556348
Number of successful extensions: 1420
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 1405
Number of HSP's gapped (non-prelim): 26
length of query: 494
length of database: 11,318,596
effective HSP length: 103
effective length of query: 391
effective length of database: 8,566,539
effective search space: 3349516749
effective search space used: 3349516749
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0034b.2