BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0023.7
(362 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g61080 unknown 32 0.41
At3g26380 unknown protein 31 1.2
At3g07980 putative MAP3K epsilon protein kinase 31 1.2
At4g07650 hypothetical protein 30 1.6
At2g39740 hypothetical protein 30 2.1
At2g28510 DOF zinc finger like protein 30 2.1
At3g30520 hypothetical protein 30 2.7
At3g27640 unknown protein 30 2.7
At4g25860 unknown protein 29 3.5
At5g28280 CER1-like protein 28 6.0
At5g01170 unknown protein 28 6.0
At1g77880 hypothetical protein 28 6.0
At3g45890 unknown protein 28 7.8
At3g13300 unknown protein 28 7.8
>At3g61080 unknown
Length = 326
Score = 32.3 bits (72), Expect = 0.41
Identities = 22/96 (22%), Positives = 39/96 (39%), Gaps = 5/96 (5%)
Query: 171 GFIYDLSNSQDGHNPPCLTISTCYAGHEQEQYNSTWLT---VTFY--YVRVSQKTIHSHK 225
G ++ + + D +N P + CY GH + + +W +FY Y +V K K
Sbjct: 229 GDLWSGNIAYDKNNEPVILDPACYYGHNEADFGMSWCAGFGESFYNAYFKVMPKQAGYEK 288
Query: 226 DSCLDKDYYLYNTLRYSQEQYNSALLTVTSYYTRSI 261
L Y+ N Y S+ +++ Y R +
Sbjct: 289 RRDLYLLYHYLNHYNLFGSGYRSSAMSIIDDYLRML 324
>At3g26380 unknown protein
Length = 647
Score = 30.8 bits (68), Expect = 1.2
Identities = 33/112 (29%), Positives = 49/112 (43%), Gaps = 16/112 (14%)
Query: 183 HNPPCLTISTCYA---GHEQEQYNSTWLTVTFYYVRVSQKTIHSHKDSCLDKDYYLYNT- 238
H+P IST +A +EQ +TW V Q + H L+K + LYN
Sbjct: 414 HHPTGKNISTKHAFGLTSCKEQKANTWFIVD---KNRGQICWNQHSSEKLEKPFCLYNRK 470
Query: 239 --------LRYSQEQYNSALLTVTSYYTRSILPGTSSQCLTSKECHPHSVSP 282
L+++Q Y L T+ +S L +S Q LTSK+ ++SP
Sbjct: 471 ALLASDKKLKHNQ-LYQGKLHLHTNDKAQSCLAASSQQKLTSKDYSQGALSP 521
>At3g07980 putative MAP3K epsilon protein kinase
Length = 1367
Score = 30.8 bits (68), Expect = 1.2
Identities = 21/73 (28%), Positives = 36/73 (48%), Gaps = 10/73 (13%)
Query: 80 QMSPISATLILLTHGVQVLVRVLNSNHFAPVPPRMANHHEPVVDHLAVWRPSNHKQIDKQ 139
Q SP++ + + G+ VLV L +++ A H E V HLA+ ++ K
Sbjct: 696 QSSPLTLQMFISCRGIPVLVGFLEADY--------AKHREMV--HLAIDGMWQVFKLKKS 745
Query: 140 TSKQDSARVNSRN 152
TS+ D R+ ++N
Sbjct: 746 TSRNDFCRIAAKN 758
>At4g07650 hypothetical protein
Length = 267
Score = 30.4 bits (67), Expect = 1.6
Identities = 17/74 (22%), Positives = 30/74 (39%), Gaps = 11/74 (14%)
Query: 65 AGERCFGGGGWEKTNQMSPISATLILLTHGVQVLVRVLNSNHFAPVPPRMANHHEPVVDH 124
A + G G ++ + P+S+T ++NH P R H P +DH
Sbjct: 95 AVDELIGRSGPDRIKPLDPLSSTCF-----------TTSTNHSTPSSSRRPRHFAPPLDH 143
Query: 125 LAVWRPSNHKQIDK 138
+A + +H I +
Sbjct: 144 MAEYHQLHHLTITR 157
>At2g39740 hypothetical protein
Length = 474
Score = 30.0 bits (66), Expect = 2.1
Identities = 20/84 (23%), Positives = 37/84 (43%), Gaps = 5/84 (5%)
Query: 274 ECHPHSVSPVVAAAHPLD----VRNLGSHYHLKVFY-IRLIHYEVHLQNRSLICSFVPKT 328
EC+ +S+ ++ H + +L S +H + +R +H + QN+ + ++
Sbjct: 297 ECNRNSIIGILTGQHIQESLYRTISLPSQHHANGMHNVRNLHGQARPQNQQMQQNWSQSY 356
Query: 329 TTSRDPHLPPSNPSRKSFDPQHNN 352
T PH PP SR + NN
Sbjct: 357 NTPNPPHWPPLTQSRPQQNWTQNN 380
>At2g28510 DOF zinc finger like protein
Length = 288
Score = 30.0 bits (66), Expect = 2.1
Identities = 16/55 (29%), Positives = 22/55 (39%)
Query: 149 NSRNKAGHLGPNTGLVFNHFCSGFIYDLSNSQDGHNPPCLTISTCYAGHEQEQYN 203
NS N G G G N C GF+ N+ T+ G+E++Q N
Sbjct: 177 NSTNDVGIFGGQNGTYNNSLCYGFMSGNGNNNQNEIKMASTLGMSLEGNERKQEN 231
>At3g30520 hypothetical protein
Length = 305
Score = 29.6 bits (65), Expect = 2.7
Identities = 19/51 (37%), Positives = 23/51 (44%), Gaps = 6/51 (11%)
Query: 7 FNNSAPTLQTGERPSTTRTRHGGSDLSKSWSMTAVLFQIWTSVGSGGGGRR 57
F NSA Q RP+ T GS +S + V S+GSG GG R
Sbjct: 71 FQNSAARRQQRGRPNFQSTARRGSSAQRSGGTSRV------SIGSGSGGSR 115
>At3g27640 unknown protein
Length = 535
Score = 29.6 bits (65), Expect = 2.7
Identities = 20/75 (26%), Positives = 33/75 (43%), Gaps = 9/75 (12%)
Query: 227 SCLDKDYYLYNTLRYSQEQYNS-ALLTVTSYYTRS--------ILPGTSSQCLTSKECHP 277
SC D YLYNTLR + S + + S++ R+ +L G+S + +
Sbjct: 310 SCKDNRIYLYNTLRLDKGPVQSFSGCRIDSFFVRTMISPDGEYVLSGSSDGNAYIWQVNK 369
Query: 278 HSVSPVVAAAHPLDV 292
V P++ H +V
Sbjct: 370 PQVDPIILKGHDFEV 384
>At4g25860 unknown protein
Length = 386
Score = 29.3 bits (64), Expect = 3.5
Identities = 16/41 (39%), Positives = 24/41 (58%), Gaps = 4/41 (9%)
Query: 166 NHFCSGFIYDLSNSQDGHNPPCLTISTCYAGHEQEQYNSTW 206
+H +G+I L+ Q H+PP +S +A HEQE + TW
Sbjct: 123 HHVSNGYINVLTE-QVMHHPP---VSALHATHEQENIDVTW 159
>At5g28280 CER1-like protein
Length = 317
Score = 28.5 bits (62), Expect = 6.0
Identities = 48/218 (22%), Positives = 82/218 (37%), Gaps = 40/218 (18%)
Query: 121 VVDHLAVWRPSNHKQIDKQTSKQDSARVNSRNKAGHLGPNTGLVFNHFCSGFIYDLSNSQ 180
V+ L V +P +Q+D++ S D ++FN F+Y ++N
Sbjct: 21 VLKTLTVDKPIEFEQVDREQSWDDQ-----------------IIFNTL---FMYLVNNKL 60
Query: 181 DGHN--PPCLTISTCYAGHEQEQYNSTWLTVTFYYVRVSQKTIHSHKDSCLDKDYYLYNT 238
G + PP + H + V F Y +++H H L Y+ ++
Sbjct: 61 PGCSRIPPKPPSCASFLLHA--------VPVEFLYYWF-HRSLHHH---FLYSRYHSHHH 108
Query: 239 LRYSQEQYNSALLTVTSYYTRSILPGTSSQCLTSKECHPHSVSPVVAAAHPLDVRNLGSH 298
E S + + S+L + +T C S+ P VA +D N H
Sbjct: 109 SSIVTEPITSVVHPFAEHIAYSVL--FAIPIVTVSLCGILSIVPFVAYVTYIDFMNYMGH 166
Query: 299 YHLKVFYIRLIHYEVHLQNRSLICSFVPKTTTSRDPHL 336
Y+ + F RL H+ + S + P+T+T P L
Sbjct: 167 YNFEFFPKRLFHHSL----SSSSSATPPRTSTLYIPTL 200
>At5g01170 unknown protein
Length = 568
Score = 28.5 bits (62), Expect = 6.0
Identities = 17/32 (53%), Positives = 17/32 (53%), Gaps = 12/32 (37%)
Query: 47 TSVGSGGGGRRLAAMAEAAGERCFGGGGWEKT 78
TS GSGGGG G GGGGWEKT
Sbjct: 530 TSGGSGGGG--------GGG----GGGGWEKT 549
>At1g77880 hypothetical protein
Length = 130
Score = 28.5 bits (62), Expect = 6.0
Identities = 25/88 (28%), Positives = 35/88 (39%), Gaps = 16/88 (18%)
Query: 236 YNTLRYSQEQYNSALLTVTSYYTRSILPGTSSQCL----TSKE-----------CHPHSV 280
Y TL +++ S L + Y TR +L GTS CL T + CH
Sbjct: 41 YPTLSLVSKRFRSLLASTELYETRRLL-GTSESCLYFFMTERRNDHEVYGKVEWCHVVHT 99
Query: 281 SPVVAAAHPLDVRNLGSHYHLKVFYIRL 308
P+ + L + SH L + YI L
Sbjct: 100 LPLFNLSRALSCYHCLSHCFLMIIYIIL 127
>At3g45890 unknown protein
Length = 608
Score = 28.1 bits (61), Expect = 7.8
Identities = 14/37 (37%), Positives = 20/37 (53%), Gaps = 1/37 (2%)
Query: 38 MTAVLFQIWTSVGSGGG-GRRLAAMAEAAGERCFGGG 73
+T V Q + +G+ G GR AA+ +AA CF G
Sbjct: 300 LTPVFPQFFVMIGAAAGAGRSAAALIQAATRSCFNAG 336
>At3g13300 unknown protein
Length = 1326
Score = 28.1 bits (61), Expect = 7.8
Identities = 13/36 (36%), Positives = 21/36 (58%)
Query: 94 GVQVLVRVLNSNHFAPVPPRMANHHEPVVDHLAVWR 129
G +L + N+N+ APV + +H PVV+H + R
Sbjct: 106 GTHILALLNNTNNGAPVANQEPSHQLPVVNHNEIAR 141
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.318 0.132 0.414
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,992,862
Number of Sequences: 26719
Number of extensions: 385636
Number of successful extensions: 1046
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 1044
Number of HSP's gapped (non-prelim): 14
length of query: 362
length of database: 11,318,596
effective HSP length: 101
effective length of query: 261
effective length of database: 8,619,977
effective search space: 2249813997
effective search space used: 2249813997
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0023.7