BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0023.12
(152 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g28840 unknown protein 31 0.30
At1g15340 unknown protein 29 0.86
At4g12780 auxilin-like protein 28 1.5
At1g49760 Putative Poly-A Binding Protein (F14J22.3) 28 1.9
At5g56980 unknown protein 28 2.5
At5g07520 glycine-rich protein atGRP 28 2.5
At5g07600 unknown protein 27 5.6
At3g28780 histone-H4-like protein 27 5.6
At2g45800 LIM-domain like protein 27 5.6
At5g14300 prohibitin - like protein 26 7.3
At4g24240 putative DNA-binding protein 26 9.5
At3g61230 LIM domain protein 26 9.5
At3g59020 putative protein 26 9.5
At3g22810 unknown protein 26 9.5
At2g31660 importin (nuclear transport factor ) like protein 26 9.5
At1g52000 26 9.5
>At3g28840 unknown protein
Length = 391
Score = 30.8 bits (68), Expect = 0.30
Identities = 18/42 (42%), Positives = 20/42 (46%)
Query: 2 ATTVQEATQNKRGSGAAGTEAEAAAAAEGMEAATARTAEAGG 43
A T T + G+ A GT A A AA G AA A AGG
Sbjct: 282 AGTAAGTTASGAGTAAGGTTAAGAGAAAGAGAAAGAGAAAGG 323
Score = 28.1 bits (61), Expect = 1.9
Identities = 22/50 (44%), Positives = 23/50 (46%), Gaps = 1/50 (2%)
Query: 3 TTVQEATQNKRGSGAAGTEAEA-AAAAEGMEAATARTAEAGGGEGFERSS 51
TT A G+ AAG A A A AA G AA T AGG G SS
Sbjct: 288 TTASGAGTAAGGTTAAGAGAAAGAGAAAGAGAAAGGTTAAGGVSGSYGSS 337
Score = 27.3 bits (59), Expect = 3.3
Identities = 13/30 (43%), Positives = 16/30 (53%)
Query: 14 GSGAAGTEAEAAAAAEGMEAATARTAEAGG 43
G+GA G+ A + A G AT T AGG
Sbjct: 212 GAGAGGSSASGSDTAAGGTTATGGTTAAGG 241
>At1g15340 unknown protein
Length = 384
Score = 29.3 bits (64), Expect = 0.86
Identities = 18/54 (33%), Positives = 24/54 (44%), Gaps = 4/54 (7%)
Query: 3 TTVQEATQNKRGSGAAGTEAEAAAAAEGMEAATARTAE----AGGGEGFERSSR 52
T + EA + K G A EA + E+ T+E AG GEG E S+
Sbjct: 184 TEIAEAEKEKEGEKAEAENKEAEVVRDKKESMEVDTSELEKKAGSGEGAEEPSK 237
>At4g12780 auxilin-like protein
Length = 904
Score = 28.5 bits (62), Expect = 1.5
Identities = 24/61 (39%), Positives = 27/61 (43%), Gaps = 4/61 (6%)
Query: 5 VQEATQNKRGSGAAGTEAEAA-AAAEGMEAATARTAEA---GGGEGFERSSRSRLCREVA 60
VQ A R AAG +AA AAAE E A AEA E E+ +R R R
Sbjct: 592 VQRAHAEARERAAAGARDKAAKAAAEAREKAEKAAAEAKERANAEAREKETRVRAERAAV 651
Query: 61 E 61
E
Sbjct: 652 E 652
>At1g49760 Putative Poly-A Binding Protein (F14J22.3)
Length = 671
Score = 28.1 bits (61), Expect = 1.9
Identities = 18/40 (45%), Positives = 20/40 (50%), Gaps = 2/40 (5%)
Query: 5 VQEATQNKRGSGAA--GTEAEAAAAAEGMEAATARTAEAG 42
+Q QN G A AEAAAAA G AA A A+ G
Sbjct: 4 IQHQGQNANGGVAVPGAAAAEAAAAAAGAAAAAAGAAQQG 43
>At5g56980 unknown protein
Length = 379
Score = 27.7 bits (60), Expect = 2.5
Identities = 19/74 (25%), Positives = 29/74 (38%), Gaps = 4/74 (5%)
Query: 3 TTVQEATQNKRGSGAAGTEAEAAAAAEGMEAATARTAEAGGGEGFERSSRSRLCREVAEC 62
T + ++ K G G AG+ AEA E +E T F A+
Sbjct: 295 TKMTKSASEKSGFGFAGSHAEAPETVESLERRRPDTTRVERSTSFGDGEDG----VDAKA 350
Query: 63 GQYINRYKSLLPLR 76
+IN++K L L+
Sbjct: 351 SDFINKFKQQLKLQ 364
>At5g07520 glycine-rich protein atGRP
Length = 228
Score = 27.7 bits (60), Expect = 2.5
Identities = 15/31 (48%), Positives = 16/31 (51%)
Query: 14 GSGAAGTEAEAAAAAEGMEAATARTAEAGGG 44
G GAAG +A AA A EAA A A G
Sbjct: 167 GPGAAGGDASAAGGAPAAEAAPAAGAAPAAG 197
>At5g07600 unknown protein
Length = 230
Score = 26.6 bits (57), Expect = 5.6
Identities = 16/33 (48%), Positives = 19/33 (57%), Gaps = 1/33 (3%)
Query: 14 GSGAAGTEAEAAAAAEGMEAATARTAEAGGGEG 46
G+GAA AAAA G+ AA+A A G G G
Sbjct: 181 GAGAAAAAGAAAAAVPGLGAASA-GAGPGAGAG 212
Score = 26.2 bits (56), Expect = 7.3
Identities = 16/40 (40%), Positives = 17/40 (42%)
Query: 7 EATQNKRGSGAAGTEAEAAAAAEGMEAATARTAEAGGGEG 46
EA G+ AAG A AAA A A AG G G
Sbjct: 169 EAAGAGAGAAAAGAGAAAAAGAAAAAVPGLGAASAGAGPG 208
>At3g28780 histone-H4-like protein
Length = 614
Score = 26.6 bits (57), Expect = 5.6
Identities = 14/45 (31%), Positives = 20/45 (44%)
Query: 2 ATTVQEATQNKRGSGAAGTEAEAAAAAEGMEAATARTAEAGGGEG 46
A+ T + G A+G AE + AA+ +AE GG G
Sbjct: 293 ASGASAKTGGESGEAASGGSAETGGESASAGAASGGSAETGGESG 337
Score = 26.6 bits (57), Expect = 5.6
Identities = 21/57 (36%), Positives = 28/57 (48%), Gaps = 7/57 (12%)
Query: 2 ATTVQEATQNKRGSGAA-GTEAEAAAAAEGME------AATARTAEAGGGEGFERSS 51
A T E+T + SG + G+E+ +A AA G AA + EAG G E SS
Sbjct: 435 AETGGESTSSGVASGGSTGSESASAGAASGGSTEANGGAAAGGSTEAGSGTSTETSS 491
>At2g45800 LIM-domain like protein
Length = 226
Score = 26.6 bits (57), Expect = 5.6
Identities = 10/33 (30%), Positives = 16/33 (48%)
Query: 91 SSCHLNRCKRGAVKALFWCSVELDSLIVEAGSY 123
S C L ++ + +C V + L +E GSY
Sbjct: 136 SGCPLTHSSYASLNGVLYCKVHFNQLFLEKGSY 168
>At5g14300 prohibitin - like protein
Length = 249
Score = 26.2 bits (56), Expect = 7.3
Identities = 21/58 (36%), Positives = 25/58 (42%), Gaps = 3/58 (5%)
Query: 6 QEATQNKRGSGAAGTEAEAAAAAEGMEAATART---AEAGGGEGFERSSRSRLCREVA 60
QEA ++K A E AA E+ AR A AG G G + R REVA
Sbjct: 162 QEAERSKFVVAKADQERRAAVIRAEGESEAARVISKATAGAGMGLIKLRRVEAAREVA 219
>At4g24240 putative DNA-binding protein
Length = 353
Score = 25.8 bits (55), Expect = 9.5
Identities = 16/45 (35%), Positives = 19/45 (41%)
Query: 4 TVQEATQNKRGSGAAGTEAEAAAAAEGMEAATARTAEAGGGEGFE 48
TV+ + G G G A AAA ME R A + G G E
Sbjct: 2 TVELMMSSYSGGGGGGDGFPAIAAAAKMEDTALREAASAGIHGVE 46
>At3g61230 LIM domain protein
Length = 213
Score = 25.8 bits (55), Expect = 9.5
Identities = 10/33 (30%), Positives = 15/33 (45%)
Query: 91 SSCHLNRCKRGAVKALFWCSVELDSLIVEAGSY 123
S C L A+ + +C V L +E G+Y
Sbjct: 137 SGCPLTHSSYAALDGVLYCKVHFSQLFLEKGNY 169
>At3g59020 putative protein
Length = 1112
Score = 25.8 bits (55), Expect = 9.5
Identities = 11/29 (37%), Positives = 19/29 (64%)
Query: 117 IVEAGSYLTNQDPNRSIELPNLFPALYEA 145
++E SY+T P S+E+ +L+P + EA
Sbjct: 740 VLEIVSYITTFSPTISLEMWSLWPLMMEA 768
>At3g22810 unknown protein
Length = 472
Score = 25.8 bits (55), Expect = 9.5
Identities = 22/90 (24%), Positives = 37/90 (40%)
Query: 17 AAGTEAEAAAAAEGMEAATARTAEAGGGEGFERSSRSRLCREVAECGQYINRYKSLLPLR 76
AA + A AAA + AATA ++ AG E ++ + Q + + + R
Sbjct: 197 AAVSVAGVAAAVAAIAAATAASSSAGKDENMAKTDMAVASAATLVAAQCVEAAEVMGAER 256
Query: 77 VFVFTAPSKRTAVKSSCHLNRCKRGAVKAL 106
+ + S V+S+ + GA AL
Sbjct: 257 DHLASVVSSAVNVRSAGDIMTLTAGAATAL 286
>At2g31660 importin (nuclear transport factor ) like protein
Length = 1040
Score = 25.8 bits (55), Expect = 9.5
Identities = 10/29 (34%), Positives = 20/29 (68%)
Query: 117 IVEAGSYLTNQDPNRSIELPNLFPALYEA 145
++E SY+T P+ S+++ +L+P + EA
Sbjct: 662 VLEIASYMTFYSPSISLDIWSLWPLMVEA 690
>At1g52000
Length = 730
Score = 25.8 bits (55), Expect = 9.5
Identities = 19/61 (31%), Positives = 27/61 (44%), Gaps = 13/61 (21%)
Query: 4 TVQEATQNKRGSGAAGTEAEAAAAAEGM-------------EAATARTAEAGGGEGFERS 50
T + A +K SG+AGT A+A G +A T A AGG G E++
Sbjct: 192 TEKNAGGSKPSSGSAGTNPGASAGGNGETEKNVGGSKPSSGKAGTNPGANAGGNGGTEKN 251
Query: 51 S 51
+
Sbjct: 252 A 252
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.316 0.129 0.371
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,926,385
Number of Sequences: 26719
Number of extensions: 104267
Number of successful extensions: 399
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 368
Number of HSP's gapped (non-prelim): 34
length of query: 152
length of database: 11,318,596
effective HSP length: 90
effective length of query: 62
effective length of database: 8,913,886
effective search space: 552660932
effective search space used: 552660932
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0023.12