BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0022.15
(313 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g63020 RNA polymerase IIA largest subunit, putative 33 0.20
At5g49520 unknown protein 32 0.45
At5g27220 putative protein 31 0.99
At2g10960 pseudogene; similar to MURA transposase of maize Muta... 30 1.7
At5g49290 disease resistance protein-like 30 2.2
At5g65170 putative protein 29 2.9
At3g60850 unknown protein 29 3.8
At5g43560 unknown protein 28 4.9
At3g21880 hypothetical protein 28 4.9
At5g27340 hypothetical protein 28 6.4
At2g44190 unknown protein 28 6.4
At1g63370 flavin-binding monooxygenase, putative 28 6.4
At1g62620 similar to flavin-binding monooxygenase (Z71258); simi... 28 6.4
At3g47460 chromosome assembly protein homolog 28 8.4
At2g24520 putative plasma membrane proton ATPase 28 8.4
At1g75730 hypothetical protein 28 8.4
>At1g63020 RNA polymerase IIA largest subunit, putative
Length = 1453
Score = 33.1 bits (74), Expect = 0.20
Identities = 34/125 (27%), Positives = 57/125 (45%), Gaps = 9/125 (7%)
Query: 6 KTTEFHHNQSETQHSILTKVTRSSIYSEQLSEQRSSKK--RRKSSSPLLL-LKLTGD--- 59
K F N E ++ + S ++SE +S + S ++KS +P L L+ D
Sbjct: 255 KLVGFEGNTLELSSRVMECMQYSRLFSETVSSSKDSANPYQKKSDTPKLCGLRFMKDVLL 314
Query: 60 GKMRENENFAILIFPAPSSVNSKIGFGDRIPKLKRGFLELSGCRKRKLPSE--ERLETSR 117
GK R + F ++ PS ++IG + I K + L+ C K +L + L ++
Sbjct: 315 GK-RSDHTFRTVVVGDPSLKLNEIGIPESIAKRLQVSEHLNQCNKERLVTSFVPTLLDNK 373
Query: 118 EMGVR 122
EM VR
Sbjct: 374 EMHVR 378
>At5g49520 unknown protein
Length = 399
Score = 32.0 bits (71), Expect = 0.45
Identities = 27/97 (27%), Positives = 36/97 (36%)
Query: 3 LRAKTTEFHHNQSETQHSILTKVTRSSIYSEQLSEQRSSKKRRKSSSPLLLLKLTGDGKM 62
+ K E HH+Q + Q K T + I EQ EQ+ + SSS + L + D
Sbjct: 1 MEKKKEEDHHHQQQQQQQKEIKNTETKIEQEQEQEQKQEISQASSSSNMANLVTSSDHHP 60
Query: 63 RENENFAILIFPAPSSVNSKIGFGDRIPKLKRGFLEL 99
E IF S F D FL+L
Sbjct: 61 LELAGNLSSIFDTSSLPFPYSYFEDHSSNNPNSFLDL 97
>At5g27220 putative protein
Length = 1181
Score = 30.8 bits (68), Expect = 0.99
Identities = 29/140 (20%), Positives = 62/140 (43%), Gaps = 9/140 (6%)
Query: 9 EFHHNQSETQHSILTKVTRSSIYSEQLSEQRSSKKRRKSSSPLLLLKLTGDGKMRENENF 68
E H+ +E + ++ +QLSE++ SK+ + S+ L + T + +ENE
Sbjct: 455 ERHNEIAEAVRKLSLEIVSKEKTIQQLSEKQHSKQTKLDSTEKCLEETTAELVSKENELC 514
Query: 69 AILIFPAPSSVNSKI------GFGDRIPKLKRGFLELSGCRKRKLPSEERL-ETSREMGV 121
++ N +I F + + K++ + + +E L E +E+G+
Sbjct: 515 SVKDTYRECLQNWEIKEKELKSFQEEVKKIQDSLKDFQSKEAELVKLKESLTEHEKELGL 574
Query: 122 RVWNLHLKLRIEKVFINQEK 141
+ +H +R EK+ + +K
Sbjct: 575 KKKQIH--VRSEKIELKDKK 592
>At2g10960 pseudogene; similar to MURA transposase of maize Mutator
transposon
Length = 362
Score = 30.0 bits (66), Expect = 1.7
Identities = 18/76 (23%), Positives = 36/76 (46%), Gaps = 1/76 (1%)
Query: 14 QSETQHSILTKVTRSSIYSEQLSEQRSSKKRRKSSSPLLLLKLTGDGKMRENENFAILIF 73
++ET ++ K+ I ++S++ S ++RR +L D + E E +
Sbjct: 236 ETETVEGVIDKIETLKIEQREISDESSEEQRRSFRDEEILNFFIPDRVLPEPEPDPLTRM 295
Query: 74 PAPSSVNSKIGFGDRI 89
P PS ++ K FG ++
Sbjct: 296 PTPSFLSGK-KFGSKV 310
>At5g49290 disease resistance protein-like
Length = 888
Score = 29.6 bits (65), Expect = 2.2
Identities = 18/54 (33%), Positives = 24/54 (44%), Gaps = 4/54 (7%)
Query: 185 LGKQHGFIIVITRSDNGGLKRKTFMILGCERCDKYIPYKEVLKHQSTGTKKCYC 238
LG HGF I + L+ K F++ CE C+ Y VL + TK C
Sbjct: 4 LGHLHGFSSCIEKERKALLELKKFVMSRCEECE----YDSVLPTWTNDTKSDCC 53
>At5g65170 putative protein
Length = 362
Score = 29.3 bits (64), Expect = 2.9
Identities = 14/37 (37%), Positives = 18/37 (47%), Gaps = 3/37 (8%)
Query: 259 YHIHERSETLL---GHPYVGRPFTNKRLADGPRPVRL 292
YH H + + LL P + PF N L D P+P L
Sbjct: 226 YHHHHQHQNLLLNMNTPNISNPFLNNSLTDQPKPSSL 262
>At3g60850 unknown protein
Length = 648
Score = 28.9 bits (63), Expect = 3.8
Identities = 20/83 (24%), Positives = 39/83 (46%), Gaps = 9/83 (10%)
Query: 168 TDKVFASRDEILEWARNLGKQHGFIIVITRSDNGGLKRKTFMI----LGCERCDKYIPYK 223
T+ + E+ + + L +H ++ I + ++ K FM C R + + K
Sbjct: 269 TESLDVPAKEVPDRLQQLAAKHKVVLCIRQKY---IRYKPFMAEVKDYACHRAGEPVSIK 325
Query: 224 E--VLKHQSTGTKKCYCPFRLRA 244
+ +LK + +K+C C FR+RA
Sbjct: 326 KSRILKREPYQSKRCGCGFRIRA 348
>At5g43560 unknown protein
Length = 1055
Score = 28.5 bits (62), Expect = 4.9
Identities = 22/74 (29%), Positives = 36/74 (47%), Gaps = 18/74 (24%)
Query: 89 IPKLKRGFLELSGCRKRKLPSEERLETSREMGVRVWN-LHLKLRIEKV------------ 135
+PKLK GF++ SGC L + +++ RE R + LH K R E V
Sbjct: 171 LPKLKEGFIDDSGC----LTIKAQVQVIRERVDRPFRCLHYKYREELVRVYLGNVEQICW 226
Query: 136 -FINQEKPLTGKLV 148
F+ +++ G+L+
Sbjct: 227 RFVEEKRSKLGRLI 240
>At3g21880 hypothetical protein
Length = 364
Score = 28.5 bits (62), Expect = 4.9
Identities = 21/71 (29%), Positives = 29/71 (40%), Gaps = 6/71 (8%)
Query: 213 CERCDKYIPYKEVLKHQSTGTKKCYCPFRLRARGTKVGWKVSVMNGYHIHERSETLLGH- 271
C CD ++ L H+ + C F A + KVS G H HE + + LGH
Sbjct: 25 CLNCDVHVHSANPLSHRHIRSLICEKCFSQPAAIRCLDEKVSYCQGCHWHESNCSELGHR 84
Query: 272 -----PYVGRP 277
P+ G P
Sbjct: 85 VQSLNPFSGCP 95
>At5g27340 hypothetical protein
Length = 238
Score = 28.1 bits (61), Expect = 6.4
Identities = 17/47 (36%), Positives = 24/47 (50%), Gaps = 2/47 (4%)
Query: 87 DRIPKLKRGFLELSGCRKRKLPSEERLE--TSREMGVRVWNLHLKLR 131
+++PK + G LEL G KR L RLE +E +VW + R
Sbjct: 29 EKLPKAEDGELELGGMGKRSLHKGRRLEHRILKEHVRQVWEIGRSFR 75
>At2g44190 unknown protein
Length = 474
Score = 28.1 bits (61), Expect = 6.4
Identities = 14/48 (29%), Positives = 23/48 (47%)
Query: 11 HHNQSETQHSILTKVTRSSIYSEQLSEQRSSKKRRKSSSPLLLLKLTG 58
HH+Q + Q S + R + ++ E R S+ R SP L ++ G
Sbjct: 69 HHHQHQNQRSTSAQRMRRQLKMQEGDENRPSETARSLDSPFPLQQVDG 116
>At1g63370 flavin-binding monooxygenase, putative
Length = 450
Score = 28.1 bits (61), Expect = 6.4
Identities = 12/40 (30%), Positives = 23/40 (57%)
Query: 135 VFINQEKPLTGKLVRAEDVQDEPLAVDYTQSFTTDKVFAS 174
V ++K + G + ++V+ +PL+VD T+S V+ S
Sbjct: 37 VVFEKQKQVGGTWIYTDEVESDPLSVDPTRSVVHSSVYRS 76
>At1g62620 similar to flavin-binding monooxygenase (Z71258); similar
to ESTs gb|R30018, gb|T23015, and gb|T88100
Length = 458
Score = 28.1 bits (61), Expect = 6.4
Identities = 12/40 (30%), Positives = 23/40 (57%)
Query: 135 VFINQEKPLTGKLVRAEDVQDEPLAVDYTQSFTTDKVFAS 174
V ++K + G + ++V+ +PL+VD T+S V+ S
Sbjct: 37 VVFEKQKQVGGTWIYTDEVESDPLSVDPTRSVVHSSVYRS 76
>At3g47460 chromosome assembly protein homolog
Length = 1171
Score = 27.7 bits (60), Expect = 8.4
Identities = 45/215 (20%), Positives = 79/215 (35%), Gaps = 17/215 (7%)
Query: 19 HSILTKVTRSSIYSEQLSEQRSSKKRRKSSSPLLLLKLTGDGKMRENENFAILIFPAPSS 78
H + T+ + + LSE ++ K + L K T E + + + +F +
Sbjct: 674 HDLAEAETKFRAHQKSLSEIEANIKELQP----LQTKFTDMKAQLELKMYDMSLFLKRAE 729
Query: 79 VNSKIGFGDRIPKLKRGFLEL-------SGCRKRKLPSEERLETS-----REMGVRVWNL 126
N GD + KL+ E+ G K + LE S + R+ +L
Sbjct: 730 QNEHHKLGDAVKKLEEEVEEMRSQIKEKEGLYKSCADTVSTLEKSIKDHDKNREGRLKDL 789
Query: 127 HLKLRIEKVFIN-QEKPLTGKLVRAEDVQDEPLAVDYTQSFTTDKVFASRDEILEWARNL 185
++ K I K L G E + E AV QS+ ++ + R +I A ++
Sbjct: 790 EKNIKTLKARIQASSKDLKGHENVRERLVMEQEAVTQEQSYLKSQLTSLRTQISTLASDV 849
Query: 186 GKQHGFIIVITRSDNGGLKRKTFMILGCERCDKYI 220
G Q + I + + L + + CD I
Sbjct: 850 GNQRAKVDAIQKDHDQSLSELKLIHAKMKECDTQI 884
>At2g24520 putative plasma membrane proton ATPase
Length = 931
Score = 27.7 bits (60), Expect = 8.4
Identities = 14/61 (22%), Positives = 33/61 (53%), Gaps = 1/61 (1%)
Query: 139 QEKPLTGKLVRAEDVQD-EPLAVDYTQSFTTDKVFASRDEILEWARNLGKQHGFIIVITR 197
Q+ +T ++ E++ + L D T + T +K+ ++ + +A+ +GK+H F++
Sbjct: 288 QQGAITKRMTAIEEMAGMDVLCCDKTGTLTLNKLTVDKNLVEVFAKGVGKEHVFLLAARA 347
Query: 198 S 198
S
Sbjct: 348 S 348
>At1g75730 hypothetical protein
Length = 565
Score = 27.7 bits (60), Expect = 8.4
Identities = 11/48 (22%), Positives = 25/48 (51%)
Query: 5 AKTTEFHHNQSETQHSILTKVTRSSIYSEQLSEQRSSKKRRKSSSPLL 52
+ + ++HN S+ QH ++T S+ + + R+ R++ PL+
Sbjct: 502 SSSPRYNHNVSQQQHRLMTAAAAMSMSHQHNNPSRAVMNRQEHHFPLI 549
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.321 0.137 0.405
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,953,600
Number of Sequences: 26719
Number of extensions: 299049
Number of successful extensions: 753
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 746
Number of HSP's gapped (non-prelim): 16
length of query: 313
length of database: 11,318,596
effective HSP length: 99
effective length of query: 214
effective length of database: 8,673,415
effective search space: 1856110810
effective search space used: 1856110810
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0022.15