BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0020.10
(535 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g41610 Na+/H+ antiporter-like protein 39 0.006
At5g44240 ATPase, calcium-transporting 35 0.080
At1g18010 hypothetical protein 33 0.52
At1g18000 hypothetical protein 33 0.52
At5g30410 putative protein 32 0.68
At2g18480 putative sugar transporter 32 1.2
At5g50790 MtN3-like protein 30 2.6
At2g37730 hypothetical protein 30 2.6
At2g31960 glucan synthase like protein 30 3.4
At1g50310 hexose transporter, putative 30 3.4
At5g61700 ABC family transporter - like protein 30 4.4
At5g26250 hexose transporter - like protein 29 5.7
At3g45490 putative protein 29 5.7
At1g08930 ERD6 protein 29 5.7
At3g58510 ATP-dependent RNA helicase-like protein 29 7.5
At1g48510 cytochrome c oxidase assembly protein Surfeit 1, putative 28 9.8
>At5g41610 Na+/H+ antiporter-like protein
Length = 810
Score = 39.3 bits (90), Expect = 0.006
Identities = 55/240 (22%), Positives = 89/240 (36%), Gaps = 36/240 (15%)
Query: 145 GLAIAAPILGFLSFHLDGHFPK-FITVASTAVGV---FFCLPAGF--------------- 185
G+ + +LG LD FPK +TV T + FF AG
Sbjct: 67 GIMLGPSLLGRSKAFLDAVFPKKSLTVLETLANLGLLFFLFLAGLEIDTKALRRTGKKAL 126
Query: 186 -FKVTAIFIPYIAGIIAASTVATAAHTHHLGLMIRSFTGPTLKRSQFPIRQGISSKLSLH 244
+ I +P+ GI ++ + F G L + FP+ I ++L L
Sbjct: 127 GIALAGITLPFALGIGSSFVLKATISKGVNSTAFLVFMGVALSITAFPVLARILAELKLL 186
Query: 245 ATAAG--CLGSALISAFTYHMIRELNV-----NEPDIMSLWIVSIFSGLIWLVGIFHVAT 297
T G + +A ++ ++ L + N ++SLW+ SG +++G +
Sbjct: 187 TTEIGRLAMSAAAVNDVAAWILLALAIALSGSNTSPLVSLWV--FLSGCAFVIGASFIIP 244
Query: 298 AIGRTTDEISFSSRLHPFSVFKYPHAIGALASVFISSFITMTIFTGGV--VFIVGQLCIK 355
I R S R H + + LA V + FIT I + F+VG L K
Sbjct: 245 PIFRW-----ISRRCHEGEPIEETYICATLAVVLVCGFITDAIGIHSMFGAFVVGVLIPK 299
>At5g44240 ATPase, calcium-transporting
Length = 1078
Score = 35.4 bits (80), Expect = 0.080
Identities = 37/151 (24%), Positives = 66/151 (43%), Gaps = 27/151 (17%)
Query: 245 ATAAGCLGSALISAFTYHMI--RELNVNEPDIMSLWIVSIFSGLIWLVGIFHVATAIGRT 302
+T AG G +L A +I + ++ L +V++ SG IWL A + +
Sbjct: 860 STFAGWFGRSLFHAIIVFVITIHAYAYEKSEMEELGMVAL-SGCIWLQ-----AFVVAQE 913
Query: 303 TDEISFSSRLHPFSVFKYPHAIGALASVFISSFITMTIFTGGVVFIVGQLCIKPVHLLFF 362
T+ F+V ++ G L + +F+ I + G+ I+ +LC +P +
Sbjct: 914 TNS---------FTVLQHLSIWGNLVGFYAINFLFSAIPSSGMYTIMFRLCSQP----SY 960
Query: 363 WLTYFLF------PVFSLSLLQPLLRVIKMN 387
W+T FL P+F+L + R K+N
Sbjct: 961 WITMFLIVGAGMGPIFALKYFRYTYRPSKIN 991
>At1g18010 hypothetical protein
Length = 459
Score = 32.7 bits (73), Expect = 0.52
Identities = 26/125 (20%), Positives = 52/125 (40%), Gaps = 1/125 (0%)
Query: 400 LISSGFGFYYGHSHWKWGHLVVFSVIQSTGTGILHAFSRVLVLDCAPSGKEGAFSIWYAW 459
++ +G YY H H + +V + G G+L A ++ P ++G + +
Sbjct: 106 VLYAGSFLYYNHHHHQ-AFAIVAGALLGCGAGLLWAGEGAVMTSYPPPHRKGTYIALFWS 164
Query: 460 MRSAGLLVGFTVGSVSPGNIRTSFGAAFCTAIAGIVVLLFGNISDVGGAVAAGHVRDDSE 519
+ + G ++G + + ++ T IA + + G + G A +R+D
Sbjct: 165 IFNLGGVIGGLIPFILNYQRSSAASVNDSTYIAFMCFMFAGVLLSFGILPATSVIRNDGS 224
Query: 520 RSSAV 524
R SAV
Sbjct: 225 RCSAV 229
>At1g18000 hypothetical protein
Length = 459
Score = 32.7 bits (73), Expect = 0.52
Identities = 26/125 (20%), Positives = 52/125 (40%), Gaps = 1/125 (0%)
Query: 400 LISSGFGFYYGHSHWKWGHLVVFSVIQSTGTGILHAFSRVLVLDCAPSGKEGAFSIWYAW 459
++ +G YY H H + +V + G G+L A ++ P ++G + +
Sbjct: 106 VLYAGSFLYYNHHHHQ-AFAIVAGALLGCGAGLLWAGEGAVMTSYPPPHRKGTYIALFWS 164
Query: 460 MRSAGLLVGFTVGSVSPGNIRTSFGAAFCTAIAGIVVLLFGNISDVGGAVAAGHVRDDSE 519
+ + G ++G + + ++ T IA + + G + G A +R+D
Sbjct: 165 IFNLGGVIGGLIPFILNYQRSSAASVNDSTYIAFMCFMFAGVLLSFGILPATSVIRNDGS 224
Query: 520 RSSAV 524
R SAV
Sbjct: 225 RCSAV 229
>At5g30410 putative protein
Length = 433
Score = 32.3 bits (72), Expect = 0.68
Identities = 25/84 (29%), Positives = 40/84 (46%), Gaps = 6/84 (7%)
Query: 169 TVASTAVGVFFCLPAGFFKVTAIFIPYIAGI-IAASTVATAAHTHHLGLMIRSFTGPT-- 225
T + T G FF + V + + Y+ IA S + T + TH LG+++RS+ T
Sbjct: 129 TGSYTLYGGFFYFCFLWLPVPRLVLGYVTSYQIALSQITTRSLTHLLGILVRSYESETDI 188
Query: 226 ---LKRSQFPIRQGISSKLSLHAT 246
R+ IRQ + SK+ + T
Sbjct: 189 TLAHLRNLLEIRQVLKSKVYRYLT 212
>At2g18480 putative sugar transporter
Length = 508
Score = 31.6 bits (70), Expect = 1.2
Identities = 28/110 (25%), Positives = 49/110 (44%), Gaps = 19/110 (17%)
Query: 275 MSLWIVSIFSGLIWLVGIFHVAT-------AIGRTTDEISFSSRLHPFSVFKYPHAIGAL 327
+SL +V F L W + + V+T +IG +SS + P + +IG
Sbjct: 365 VSLTMVQRFGRLAWALSLSIVSTYAFVAFFSIGLGPITWVYSSEIFPLRLRAQGASIGVA 424
Query: 328 ASVFIS-----SFITMT--IFTGGVVFIVGQLCIKPVHLLFFWLTYFLFP 370
+ ++ SF++MT I TGGV F+ + + +W +F+ P
Sbjct: 425 VNRIMNATVSMSFLSMTKAITTGGVFFVFAGIAVAA-----WWFFFFMLP 469
>At5g50790 MtN3-like protein
Length = 289
Score = 30.4 bits (67), Expect = 2.6
Identities = 41/178 (23%), Positives = 81/178 (45%), Gaps = 15/178 (8%)
Query: 245 ATAAGCLGSALISAF-------TYHMIRELNVNEPDIMSLWIVSIFSGLIWL-VGIFHVA 296
AT G LG+ +IS F T+ I + +E +++S+FS ++W+ +
Sbjct: 9 ATVFGILGN-IISFFVCLAPIPTFVRIYKRKSSEGYQSIPYVISLFSAMLWMYYAMIKKD 67
Query: 297 TAIGRTTDEISFSSRLHPFSVFKYPHAIGALASVFISSFITMTIFTGGVVFIVGQLCI-- 354
+ T + +F ++ S+F + +A ++ + + + + G +F++ I
Sbjct: 68 AMMLITINSFAFVVQIVYISLFFF-YAPKKEKTLTVKFVLFVDVLGFGAIFVLTYFIIHA 126
Query: 355 -KPVHLLFFWLTYFLFPVFSLSLLQPLLRVIKMNSVKMQIVGFLFSL-ISSGFGFYYG 410
K V +L + F VF ++ L + +VIK S + G F L +S+ F+YG
Sbjct: 127 NKRVQVLGYICMVFALSVF-VAPLGIIRKVIKTKSAEFMPFGLSFFLTLSAVMWFFYG 183
>At2g37730 hypothetical protein
Length = 532
Score = 30.4 bits (67), Expect = 2.6
Identities = 27/90 (30%), Positives = 38/90 (42%), Gaps = 11/90 (12%)
Query: 145 GLAIAAPILGFLSFHLDGHFPKF---------ITVASTAVGVFFCLPAGFFKVTAIFIPY 195
G+AI+ P+ L LDG ++ I + +GV GF +V PY
Sbjct: 244 GIAISYPLAVELVKLLDGCIDRYASLYGSDQKIEACLSEIGVPLTKELGFHQVDIRGNPY 303
Query: 196 IAGIIAASTVATAAHTHHLGLMIRSFTGPT 225
G++AA VA HHL + F G T
Sbjct: 304 --GLLAAHPVAPLVTLHHLDYVDPIFPGTT 331
>At2g31960 glucan synthase like protein
Length = 1956
Score = 30.0 bits (66), Expect = 3.4
Identities = 25/114 (21%), Positives = 44/114 (37%), Gaps = 9/114 (7%)
Query: 259 FTYHMIRELNVNEPDIMSLWIVSIFSGLIWLVGIFHVATAIGRTTDEISFSSRLHPFSVF 318
F Y ++ +L+ + + SLWI +I + + +GR +F F
Sbjct: 1776 FQYGLVYQLSTFKQENQSLWIYGASWFVILFILLIVKGLGVGRQRFSTNFQLLFRIIKGF 1835
Query: 319 KYPHAIGALASVFISSFIT-------MTIF--TGGVVFIVGQLCIKPVHLLFFW 363
+ +G L + F+T M F TG + ++ Q C + L FW
Sbjct: 1836 VFLTFLGLLITFLALRFLTPKDIFLCMLAFMPTGWGMLLIAQACKPLIQRLGFW 1889
>At1g50310 hexose transporter, putative
Length = 517
Score = 30.0 bits (66), Expect = 3.4
Identities = 19/58 (32%), Positives = 31/58 (52%), Gaps = 8/58 (13%)
Query: 301 RTTDEISFSSRLHPFSVFKYPHAIGALASVFISSFIT------MTIFTGGVVFIVGQL 352
R T F ++L +F + ALAS F++S +T +++F GGV F++G L
Sbjct: 72 RETAYCKFDNQL--LQLFTSSLYLAALASSFVASAVTRKYGRKISMFVGGVAFLIGSL 127
>At5g61700 ABC family transporter - like protein
Length = 888
Score = 29.6 bits (65), Expect = 4.4
Identities = 27/95 (28%), Positives = 39/95 (40%), Gaps = 17/95 (17%)
Query: 359 LLFFWLTYFLFPVFSLSLL---QPLLRVI-------------KMNSVKMQIV-GFLFSLI 401
L F W+ LFPV SL+ Q LR+I + N +Q V F++S +
Sbjct: 330 LFFTWVVLLLFPVILTSLVYEKQERLRIIMKMHGLGDGLKYFRRNDYSIQFVFYFIYSNL 389
Query: 402 SSGFGFYYGHSHWKWGHLVVFSVIQSTGTGILHAF 436
F K + V + I GTG+L +F
Sbjct: 390 QISLAFLVSSIFSKVKTVTVIAYILVYGTGLLGSF 424
>At5g26250 hexose transporter - like protein
Length = 507
Score = 29.3 bits (64), Expect = 5.7
Identities = 21/88 (23%), Positives = 41/88 (45%), Gaps = 8/88 (9%)
Query: 419 LVVFSVIQSTGTGILHAFSRVLVLDCAPSGKEGAFSIWYAWMRSAGLL----VGFTVGSV 474
L++ ++ G G + + + + AP+ G +I + M + G+L V + S+
Sbjct: 134 LIIGRILLGFGVGFGNQAVPLFLSEIAPARLRGGLNIVFQLMVTIGILIANIVNYFTSSI 193
Query: 475 SPGNIRTSFGAAFCTAIAGIVVLLFGNI 502
P R + G A A+ +LLFG++
Sbjct: 194 HPYGWRIALGGAGIPAL----ILLFGSL 217
>At3g45490 putative protein
Length = 211
Score = 29.3 bits (64), Expect = 5.7
Identities = 15/51 (29%), Positives = 25/51 (48%)
Query: 469 FTVGSVSPGNIRTSFGAAFCTAIAGIVVLLFGNISDVGGAVAAGHVRDDSE 519
++ G VS N+ FG A C + ++ + ++SDV G +R SE
Sbjct: 34 YSKGLVSTDNVAAGFGVAICDQMDELLFEMKESVSDVEGVKVRALIRGLSE 84
>At1g08930 ERD6 protein
Length = 496
Score = 29.3 bits (64), Expect = 5.7
Identities = 16/60 (26%), Positives = 26/60 (42%)
Query: 429 GTGILHAFSRVLVLDCAPSGKEGAFSIWYAWMRSAGLLVGFTVGSVSPGNIRTSFGAAFC 488
G GI V + + AP G+F M++ G+ + F +G+ P + T G C
Sbjct: 161 GVGIFSYVIPVYIAEIAPKHVRGSFVFANQLMQNCGISLFFIIGNFIPWRLLTVVGLVPC 220
>At3g58510 ATP-dependent RNA helicase-like protein
Length = 612
Score = 28.9 bits (63), Expect = 7.5
Identities = 12/23 (52%), Positives = 13/23 (56%)
Query: 10 GGGHHQGGAQGGAPENGKGTTAT 32
GGG + GG GGAP G G T
Sbjct: 586 GGGGYGGGGYGGAPSGGYGAGVT 608
>At1g48510 cytochrome c oxidase assembly protein Surfeit 1, putative
Length = 325
Score = 28.5 bits (62), Expect = 9.8
Identities = 25/83 (30%), Positives = 34/83 (40%), Gaps = 2/83 (2%)
Query: 80 TVLIPVVFPLIISQLQNLPADWAKNNRPAGTHCSQKELHLYSKLTNQTITSNFSALEWTS 139
T L+ +F +ISQ Q + + +N PA + S E L S SAL W
Sbjct: 2 TKLVSKIFKRLISQSQYMSSS-TTSNLPAASQTSNLESQLLSSAPPPAKKKRGSALLWYL 60
Query: 140 IGW-AIGLAIAAPILGFLSFHLD 161
+G+ GL L HLD
Sbjct: 61 VGFTTYGLGETYKFLQTQVEHLD 83
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.326 0.140 0.434
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,644,789
Number of Sequences: 26719
Number of extensions: 488750
Number of successful extensions: 1996
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 13
Number of HSP's that attempted gapping in prelim test: 1991
Number of HSP's gapped (non-prelim): 18
length of query: 535
length of database: 11,318,596
effective HSP length: 104
effective length of query: 431
effective length of database: 8,539,820
effective search space: 3680662420
effective search space used: 3680662420
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0020.10