BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0019b.10
(251 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g35390 putative protein 34 0.065
At5g27870 pectin methyl-esterase - like protein 33 0.11
At3g60320 bZIP like protein 33 0.14
At3g55560 unknown protein 32 0.25
At1g60270 32 0.32
At5g49700 putative protein 31 0.55
At2g42940 hypothetical protein 31 0.55
At1g70620 unknown protein 31 0.55
At1g59265 polyprotein, putative 31 0.55
At1g58889 polyprotein, putative 31 0.55
At1g14380 unknown protein 31 0.55
At1g76500 unknown protein 31 0.72
At1g55500 unknown protein 31 0.72
At2g38400 putative beta-alanine-pyruvate aminotransferase 30 0.94
At1g63540 unknown protein 30 0.94
At2g45700 unknown protein 30 1.2
At2g28240 putative proline/hydroxyproline-rich glycoprotein 30 1.2
At3g60870 putative protein 30 1.6
At3g06290 hypothetical protein 30 1.6
At2g18840 unknown protein 30 1.6
>At4g35390 putative protein
Length = 270
Score = 34.3 bits (77), Expect = 0.065
Identities = 16/37 (43%), Positives = 20/37 (53%)
Query: 213 ASVEPVTPAVKKSSNTNSSKKSSQRGRPKGSKNKPQP 249
A P A ++ + SS RGRP GSKNKP+P
Sbjct: 13 AETTPTGGATSSATASGSSSGRRPRGRPAGSKNKPKP 49
>At5g27870 pectin methyl-esterase - like protein
Length = 732
Score = 33.5 bits (75), Expect = 0.11
Identities = 55/226 (24%), Positives = 79/226 (34%), Gaps = 35/226 (15%)
Query: 45 GEEQKEQLQVLIDSGYTSEEI--EGWKNNLIVEGILFNENQIFDNYVQNQGTRYAVTGYS 102
G KE + +I + + + + EGW+ L G+ N +F + VQN G A+T
Sbjct: 468 GRPWKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGL----NTLFYSEVQNTGPGAAIT--- 520
Query: 103 FNAQDLIQKSNNYWPFYSLNLDPRILTQT-------------NGIGACLPFLTSQQNRLS 149
WP D IL T G+ L + + S
Sbjct: 521 ---------KRVTWPGIKKLSDEEILKFTPAQYIQGDAWIPGKGVPYILGLFSGNGSTNS 571
Query: 150 GFATTSATSPTSELREITNVFVRTSPHQIIRQRASPSATVRNASSPETS--SQQCFPLTC 207
+S +S T+E + + V S SPS T + SP TS + Q
Sbjct: 572 TVTGSSLSSNTTESSDSPSTVVTPSTSPPAGHLGSPSDTPSSVVSPSTSLPAGQLGAPPA 631
Query: 208 SPGHVASVEPVTPA--VKKSSNTNSSKKSSQRGRPKGSKNKPQPHP 251
+P V S PA + S+T SS S P G P P
Sbjct: 632 TPSMVVSPSTSPPAGHLGSPSDTPSSLVSPSTSPPAGHLGSPSDTP 677
>At3g60320 bZIP like protein
Length = 796
Score = 33.1 bits (74), Expect = 0.14
Identities = 26/97 (26%), Positives = 46/97 (46%), Gaps = 10/97 (10%)
Query: 147 RLSGFATTSATS--PTSELREITNVFVRTSPHQIIRQRASPSATVRNASSPETSSQQCFP 204
R++G A +S S P S + VF+ T P + Q SP+ V SP + +P
Sbjct: 46 RITGSALSSFASGEPLSVSDQTPAVFLHTPPPPLSEQ--SPAKFVPPRFSPSPAPSSVYP 103
Query: 205 LTCSPGHVASVEPVTPAVKKSSNTNSSKKSSQRGRPK 241
+ SP +S +P +T+S+++ Q+ +P+
Sbjct: 104 PSTSPSVASSKQP------SVMSTSSNRRRKQQPKPR 134
>At3g55560 unknown protein
Length = 310
Score = 32.3 bits (72), Expect = 0.25
Identities = 21/61 (34%), Positives = 29/61 (47%), Gaps = 9/61 (14%)
Query: 188 TVRNASSPETSSQQCFPLTCSPGHVASVEPVTPAVKKSSNTNSSKKSSQRGRPKGSKNKP 247
T N+ SP T +Q + PAV+ S + S+ + RGRP GSKNKP
Sbjct: 51 TTNNSGSPNTQTQS--------QEEQNSRDEQPAVEPGSGSGSTGRRP-RGRPPGSKNKP 101
Query: 248 Q 248
+
Sbjct: 102 K 102
>At1g60270
Length = 477
Score = 32.0 bits (71), Expect = 0.32
Identities = 22/72 (30%), Positives = 34/72 (46%), Gaps = 14/72 (19%)
Query: 42 INSGEEQKEQLQVLIDSGYTSEEIEGWKNNLIVE------GILFNENQIFDNYVQ----- 90
+N G E L Y +E EGW N++IVE + F E F N+V+
Sbjct: 129 VNHGIEPYVTLHHFDHPQYLEDEYEGWLNHMIVEDFTAYADVCFRE---FGNHVKFWTTI 185
Query: 91 NQGTRYAVTGYS 102
N+G +++ GY+
Sbjct: 186 NEGNIFSIGGYN 197
>At5g49700 putative protein
Length = 276
Score = 31.2 bits (69), Expect = 0.55
Identities = 24/54 (44%), Positives = 29/54 (53%), Gaps = 6/54 (11%)
Query: 196 ETSSQQCFPLTCSPGHVASVEPVTPAVKKSSNTNSSKKSSQRGRPKGSKNKPQP 249
+ QQ LT S H++S VTP V SS + RGRP GSKNKP+P
Sbjct: 24 QQQQQQQHSLT-SHFHLSST--VTPTVDDSSIEVVRRP---RGRPPGSKNKPKP 71
>At2g42940 hypothetical protein
Length = 257
Score = 31.2 bits (69), Expect = 0.55
Identities = 14/29 (48%), Positives = 17/29 (58%)
Query: 221 AVKKSSNTNSSKKSSQRGRPKGSKNKPQP 249
AV+ S+ RGRP GSKNKP+P
Sbjct: 40 AVQPVSSIEGEMAKRPRGRPAGSKNKPKP 68
>At1g70620 unknown protein
Length = 897
Score = 31.2 bits (69), Expect = 0.55
Identities = 27/86 (31%), Positives = 43/86 (49%), Gaps = 9/86 (10%)
Query: 171 VRTSPHQIIRQRASPSATVRNASSPETSSQQCFPLTCSPG-----HVASVEPVTPAVKKS 225
V SP + R+R+SPS+ + S SS + + SPG HV+S P + K S
Sbjct: 810 VSPSPVRSRRKRSSPSSDESSDDSKRKSSSKRKNRSPSPGKSRRRHVSSRSPHS---KHS 866
Query: 226 SNTNSSKKSSQRGRPKGSKNKPQ-PH 250
+ N+ S + R K S+++ + PH
Sbjct: 867 QHKNTLYSSHDKSRSKRSRSRSRSPH 892
>At1g59265 polyprotein, putative
Length = 1466
Score = 31.2 bits (69), Expect = 0.55
Identities = 24/108 (22%), Positives = 40/108 (36%), Gaps = 11/108 (10%)
Query: 142 TSQQNRLSGFATTSATSPTSELREITNVFVRTSPHQIIRQRASPSATVRNASSPETSSQQ 201
T Q + TS +PT+E SP Q+ + ++P+ + ++ SP TS+
Sbjct: 846 TQTQTQTHSSQNTSQNNPTNE-----------SPSQLAQSLSTPAQSSSSSPSPTTSASS 894
Query: 202 CFPLTCSPGHVASVEPVTPAVKKSSNTNSSKKSSQRGRPKGSKNKPQP 249
P + P + ++N S R K KP P
Sbjct: 895 SSTSPTPPSILIHPPPPLAQIVNNNNQAPLNTHSMGTRAKAGIIKPNP 942
Score = 29.3 bits (64), Expect = 2.1
Identities = 20/70 (28%), Positives = 33/70 (46%), Gaps = 6/70 (8%)
Query: 173 TSPHQIIRQRASPSATVRN----ASSPETSSQQCFPLTCSP--GHVASVEPVTPAVKKSS 226
+ PH +SPSA RN +S+ ++S FP + P +P T + +
Sbjct: 791 SDPHHAATPPSSPSAPFRNSQVSSSNLDSSFSSSFPSSPEPTAPRQNGPQPTTQPTQTQT 850
Query: 227 NTNSSKKSSQ 236
T+SS+ +SQ
Sbjct: 851 QTHSSQNTSQ 860
>At1g58889 polyprotein, putative
Length = 1466
Score = 31.2 bits (69), Expect = 0.55
Identities = 24/108 (22%), Positives = 40/108 (36%), Gaps = 11/108 (10%)
Query: 142 TSQQNRLSGFATTSATSPTSELREITNVFVRTSPHQIIRQRASPSATVRNASSPETSSQQ 201
T Q + TS +PT+E SP Q+ + ++P+ + ++ SP TS+
Sbjct: 846 TQTQTQTHSSQNTSQNNPTNE-----------SPSQLAQSLSTPAQSSSSSPSPTTSASS 894
Query: 202 CFPLTCSPGHVASVEPVTPAVKKSSNTNSSKKSSQRGRPKGSKNKPQP 249
P + P + ++N S R K KP P
Sbjct: 895 SSTSPTPPSILIHPPPPLAQIVNNNNQAPLNTHSMGTRAKAGIIKPNP 942
Score = 29.3 bits (64), Expect = 2.1
Identities = 20/70 (28%), Positives = 33/70 (46%), Gaps = 6/70 (8%)
Query: 173 TSPHQIIRQRASPSATVRN----ASSPETSSQQCFPLTCSP--GHVASVEPVTPAVKKSS 226
+ PH +SPSA RN +S+ ++S FP + P +P T + +
Sbjct: 791 SDPHHAATPPSSPSAPFRNSQVSSSNLDSSFSSSFPSSPEPTAPRQNGPQPTTQPTQTQT 850
Query: 227 NTNSSKKSSQ 236
T+SS+ +SQ
Sbjct: 851 QTHSSQNTSQ 860
>At1g14380 unknown protein
Length = 664
Score = 31.2 bits (69), Expect = 0.55
Identities = 27/98 (27%), Positives = 45/98 (45%), Gaps = 9/98 (9%)
Query: 153 TTSATSPTSELREITNVFVRTSPHQIIRQRASPSAT-VRNASSPETSSQQCFPLTCSPGH 211
TTS T P+ E R P + +R+ ++ S +RN S + + G
Sbjct: 276 TTSGTGPSRFTAE------RNKPKRNVRKASTLSKDPLRNESDKANHNSRKSRSGSKEGS 329
Query: 212 VASV--EPVTPAVKKSSNTNSSKKSSQRGRPKGSKNKP 247
+ E +P++K+SS +N SKK++ R K K+ P
Sbjct: 330 PLEIKDEKPSPSLKRSSLSNGSKKATLRSAEKKKKDIP 367
>At1g76500 unknown protein
Length = 302
Score = 30.8 bits (68), Expect = 0.72
Identities = 13/22 (59%), Positives = 15/22 (68%)
Query: 228 TNSSKKSSQRGRPKGSKNKPQP 249
T+ S RGRP GSKNKP+P
Sbjct: 66 TSGSTGKRPRGRPPGSKNKPKP 87
>At1g55500 unknown protein
Length = 592
Score = 30.8 bits (68), Expect = 0.72
Identities = 20/52 (38%), Positives = 23/52 (43%), Gaps = 2/52 (3%)
Query: 200 QQCFPLTCSPGHVASVEPVTPAVKKSSNTNSSKKSSQRGRPKGSKNKPQPHP 251
Q FPLT S G AS P + K S TN + S+ G PKG P
Sbjct: 134 QYPFPLTASSGPFASSVPASTQSKLS--TNKAANSASAGIPKGMNGSAPVKP 183
>At2g38400 putative beta-alanine-pyruvate aminotransferase
Length = 477
Score = 30.4 bits (67), Expect = 0.94
Identities = 29/102 (28%), Positives = 46/102 (44%), Gaps = 10/102 (9%)
Query: 43 NSGEEQKEQLQVLIDSGYTSEEIEGWKNNLI--VEGILFNENQIFDNYVQNQGTRYAVTG 100
+ GE+ + LQ LI G T+ I G+ I V GI+ ++ Y+ G
Sbjct: 232 SDGEKYAKDLQDLIQYG-TTGHIAGFICEAIQGVGGIV----ELAPGYLSAAYDTVKKAG 286
Query: 101 YSFNAQDL---IQKSNNYWPFYSLNLDPRILTQTNGIGACLP 139
F A ++ ++ N+W F + N+ P I+T GIG P
Sbjct: 287 GLFIADEVQSGFARTGNFWGFEAHNVVPDIVTMAKGIGNGFP 328
>At1g63540 unknown protein
Length = 635
Score = 30.4 bits (67), Expect = 0.94
Identities = 30/104 (28%), Positives = 47/104 (44%), Gaps = 7/104 (6%)
Query: 124 DPRILTQTNGIGACLPFLTSQQNRLSGFATTSATSPTSELREITNVFVRTSPHQIIRQRA 183
+PRIL T+G GA +S + L + S S + + +++ + SP R
Sbjct: 116 EPRILLATSGSGASATSTSSTSSPLHSSSPFSFGSAPAAITSVSSGPAQ-SPASSPRLWI 174
Query: 184 SPSATVRNASSPETSSQQCFPLTCSPGHVASVEPVTPAVKKSSN 227
AT +AS+ +SS T SP H +S+ PAV S+
Sbjct: 175 DRFATSSSASATSSSS------TSSPFHSSSLLGFAPAVTSVSS 212
>At2g45700 unknown protein
Length = 723
Score = 30.0 bits (66), Expect = 1.2
Identities = 29/104 (27%), Positives = 45/104 (42%), Gaps = 9/104 (8%)
Query: 152 ATTSATSPTSELREITNVFVRTSPHQIIRQRASPSATVRNASSPETSS-----QQCFPLT 206
A + P + E+ TS H RQR +T R AS P+ + + FP
Sbjct: 295 ALSGVRDPFASSAEVQAQSHCTSGHVTERQR--DKSTTRKASEPKKPTANKLITEFFPGQ 352
Query: 207 CSPGHVASVEPVTPAVKKSSNTNSSKKSSQRGRPKGSKNKPQPH 250
+ G P P +KS + +SS+++ +R G K+K PH
Sbjct: 353 ATEGTKIRTAP-KPVAEKSPSDSSSRRAVRRNGNNG-KSKVIPH 394
>At2g28240 putative proline/hydroxyproline-rich glycoprotein
Length = 660
Score = 30.0 bits (66), Expect = 1.2
Identities = 24/87 (27%), Positives = 40/87 (45%), Gaps = 7/87 (8%)
Query: 166 ITNVFVRTSPHQIIRQRASPSATVRNASSPETSSQQCFPLTCSPGHVASVEPVTPAVKKS 225
+ N + T P ++ A+ +ATVR S + S+QQ + + H+ S P P+V +
Sbjct: 268 LANAYKSTCP---VKSAATDTATVRAPRSSQHSTQQQQAVQTN-RHMNSTAPPRPSVTAA 323
Query: 226 SNTNSS---KKSSQRGRPKGSKNKPQP 249
NS+ + S P S P+P
Sbjct: 324 EPMNSAAPPRPSVTAAEPMNSTAPPRP 350
>At3g60870 putative protein
Length = 265
Score = 29.6 bits (65), Expect = 1.6
Identities = 13/23 (56%), Positives = 15/23 (64%)
Query: 226 SNTNSSKKSSQRGRPKGSKNKPQ 248
S + KK RGRP GSKNKP+
Sbjct: 51 SGEENIKKRRPRGRPAGSKNKPK 73
>At3g06290 hypothetical protein
Length = 1713
Score = 29.6 bits (65), Expect = 1.6
Identities = 22/69 (31%), Positives = 32/69 (45%), Gaps = 7/69 (10%)
Query: 181 QRASPSATVRNASSPETSSQQCFPLTCSPGHVASVEPVTPAVKKSSNTNSSKKSSQRGRP 240
QRA P + +N P+ S + + PG V SV P+ S+ N S S Q +P
Sbjct: 80 QRAPPLSASQN---PQLSIGKPY----RPGGVQSVPPINRIPSPSAFQNPSPSSGQPYQP 132
Query: 241 KGSKNKPQP 249
G + P+P
Sbjct: 133 GGIQRIPEP 141
>At2g18840 unknown protein
Length = 281
Score = 29.6 bits (65), Expect = 1.6
Identities = 24/99 (24%), Positives = 41/99 (41%), Gaps = 6/99 (6%)
Query: 155 SATSPTSELREITNVFVRTSPHQIIRQR----ASPSATVRNASSPETSSQQCFPLTCSPG 210
S+ S E+ + N V++ P ++ R PS V ++SS Q P
Sbjct: 12 SSQSDIDEIENLINESVQSGPGTVLAARPPSPTRPSIPVSSSSSSSPFMQSNLPPLHPSS 71
Query: 211 HVASVE--PVTPAVKKSSNTNSSKKSSQRGRPKGSKNKP 247
V PV P + SN+++ + +S G P + +P
Sbjct: 72 SAQKVTHVPVPPPLPAVSNSSNFQGASAFGSPPNTLTEP 110
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.314 0.129 0.383
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,462,080
Number of Sequences: 26719
Number of extensions: 219574
Number of successful extensions: 941
Number of sequences better than 10.0: 52
Number of HSP's better than 10.0 without gapping: 25
Number of HSP's successfully gapped in prelim test: 27
Number of HSP's that attempted gapping in prelim test: 905
Number of HSP's gapped (non-prelim): 61
length of query: 251
length of database: 11,318,596
effective HSP length: 97
effective length of query: 154
effective length of database: 8,726,853
effective search space: 1343935362
effective search space used: 1343935362
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0019b.10