BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0016.10
(196 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g65490 unknown protein 32 0.16
At2g34830 WRKY-type DNA binding protein-like 32 0.16
At2g29130 putative laccase (diphenol oxidase) 28 4.1
At3g22390 unknown protein 27 5.3
At4g34030 3-methylcrotonyl-CoA carboxylase non-biotinylated subu... 27 6.9
At4g07840 putative transposon protein 27 6.9
At3g47870 putative protein 27 6.9
At3g16840 ATP-dependent RNA helicase 27 6.9
At3g09780 putative protein kinase 27 6.9
At5g61560 putative protein 27 9.1
At3g23280 unknown protein 27 9.1
At3g21215 unknown protein 27 9.1
At2g29240 hypothetical protein 27 9.1
At1g32750 unknown protein 27 9.1
At1g31460 unknown protein 27 9.1
>At5g65490 unknown protein
Length = 643
Score = 32.3 bits (72), Expect = 0.16
Identities = 28/122 (22%), Positives = 49/122 (39%), Gaps = 12/122 (9%)
Query: 80 YVKPNVWVRGMINEARVGPNVWIRNPSEHNQHNRVGASGKDTN------NLASSTGEQGA 133
Y P+V R +EA +G + + Q + G GK + NL +G
Sbjct: 285 YPMPSVSDRDAFSEAELGMKIACGMEMMYQQRKKEGEDGKGISWSKYKDNLEKYGYFEGL 344
Query: 134 LSVGTSTKRLSPNVELYQNATFSLTQNSSLIE------NTVISPPASDTDADLPEAPLAP 187
LS KRL N E Y + S ++ ++ + +++ P S+ D E P +
Sbjct: 345 LSGSKEYKRLMENAEEYHQKSSSFSRTRDIMSAPVRRIDEILALPYSEDDFKGQEVPASD 404
Query: 188 HN 189
++
Sbjct: 405 ND 406
>At2g34830 WRKY-type DNA binding protein-like
Length = 427
Score = 32.3 bits (72), Expect = 0.16
Identities = 43/176 (24%), Positives = 71/176 (39%), Gaps = 37/176 (21%)
Query: 40 KEIQSIEAPGVCNTLSIGPSNENMAQFDLPSLAKPKITSKYVKPNVWVRG---------- 89
K++ I AP N+ S G + DL A K K +K + + RG
Sbjct: 193 KKVVCIPAPAAMNSRSSG----EVVPSDL--WAWRKYGQKPIKGSPYPRGYYRCSSSKGC 246
Query: 90 ----MINEARVGPNVW-IRNPSEHN-----QHNRVGASGKDTNNLASSTGEQGALSVGTS 139
+ +R PN+ I SEHN Q N + S + +++ + + + + + T+
Sbjct: 247 SARKQVERSRTDPNMLVITYTSEHNHPWPTQRNALAGSTRSSSSSSLNPSSKSSTAAATT 306
Query: 140 TKRLSPNVELYQNATFSLTQNSSLIENTVISPPASDTDADLPEAPLAPHNEEGRRE 195
SP+ ++QN NSS E + P+S T A + N E R+E
Sbjct: 307 ----SPSSRVFQN-------NSSKDEPNNSNLPSSSTHPPFDAAAIKEENVEERQE 351
>At2g29130 putative laccase (diphenol oxidase)
Length = 573
Score = 27.7 bits (60), Expect = 4.1
Identities = 20/77 (25%), Positives = 34/77 (43%), Gaps = 7/77 (9%)
Query: 116 ASGKDTNNLASSTGEQGALSVGTSTKRLSPNVELYQNATFSLTQNSSLIENTVISPPASD 175
A G TN + QG T+T + + ++ N +F L +SL+++ + +
Sbjct: 367 AIGLGTNPCPKNQTCQGP----TNTTKFAASIN---NVSFILPNKTSLLQSYFVGKSKNV 419
Query: 176 TDADLPEAPLAPHNEEG 192
D P AP+ P N G
Sbjct: 420 FMTDFPTAPIIPFNYTG 436
>At3g22390 unknown protein
Length = 873
Score = 27.3 bits (59), Expect = 5.3
Identities = 31/133 (23%), Positives = 55/133 (41%), Gaps = 7/133 (5%)
Query: 59 SNENMAQFDLPSLAKPKITSKYVKPNVWVRGMINEARVGPNVWIRNPSEHNQHNRVGASG 118
S +Q + +KP + P V+G N P + + +PS + S
Sbjct: 632 SRPQQSQISFAANSKPMTSGS---PMQQVQGGTNHQAPSPPMLVGSPSTSSVSKNASGSP 688
Query: 119 KDTNNLASSTGEQGALSVGT-STKRLSPNVELYQNATFSLTQNS--SLIEN-TVISPPAS 174
+ T + +S+ + G S T S + S N++ A+ + +N+ S++ N T S S
Sbjct: 689 RTTASASSAANKGGQASTTTHSASQPSKNLQPASAASSAGGRNNGPSVLGNPTTSSGSKS 748
Query: 175 DTDADLPEAPLAP 187
LP+ L P
Sbjct: 749 QQQQQLPKHGLQP 761
>At4g34030 3-methylcrotonyl-CoA carboxylase non-biotinylated subunit
(MCCB)
Length = 587
Score = 26.9 bits (58), Expect = 6.9
Identities = 16/57 (28%), Positives = 26/57 (45%)
Query: 16 KEVMKIGGGVTLKFGLPLVNGVGGKEIQSIEAPGVCNTLSIGPSNENMAQFDLPSLA 72
+ VM G G G PLV G+E+ + + G ++ ++ AQ +L LA
Sbjct: 247 ESVMVKGNGTIFLAGPPLVKAATGEEVSAEDLGGATVHCTVSGVSDYFAQDELHGLA 303
>At4g07840 putative transposon protein
Length = 769
Score = 26.9 bits (58), Expect = 6.9
Identities = 12/23 (52%), Positives = 14/23 (60%)
Query: 78 SKYVKPNVWVRGMINEARVGPNV 100
SK VK VW+R I E V PN+
Sbjct: 664 SKAVKEAVWIRKFITELSVVPNI 686
>At3g47870 putative protein
Length = 328
Score = 26.9 bits (58), Expect = 6.9
Identities = 22/83 (26%), Positives = 31/83 (36%), Gaps = 12/83 (14%)
Query: 108 HNQHNRVGASGKDTNNLASS-TGEQGALSVGTSTKRLSPNVEL-----------YQNATF 155
HN + + G D N T +Q L +G + NV Q
Sbjct: 153 HNHNQMIHELGSDHNKQQEDVTSQQLDLGMGLNVNNNQSNVVTPFFSSLLPVSETQQPQM 212
Query: 156 SLTQNSSLIENTVISPPASDTDA 178
S T + S + N SPPA +TD+
Sbjct: 213 SYTYSCSEVNNNGYSPPAYNTDS 235
>At3g16840 ATP-dependent RNA helicase
Length = 832
Score = 26.9 bits (58), Expect = 6.9
Identities = 19/73 (26%), Positives = 29/73 (39%), Gaps = 18/73 (24%)
Query: 2 IVSWMGRSFAGPIQKEVMKIGGGVTLKFGLPLVN-------------GVGGKEIQSIEAP 48
+ ++ G+ G + G G TL FGLP++ + G+E Q A
Sbjct: 223 VAAYQGKDVIGAAET-----GSGKTLAFGLPILQRLLDEREKVGKLYALKGEEAQKYAAD 277
Query: 49 GVCNTLSIGPSNE 61
G L I P+ E
Sbjct: 278 GYLRALIITPTRE 290
>At3g09780 putative protein kinase
Length = 775
Score = 26.9 bits (58), Expect = 6.9
Identities = 19/67 (28%), Positives = 29/67 (42%), Gaps = 13/67 (19%)
Query: 21 IGGGVTLKFGLPLVNGVGGKEIQSIEAPGVCNTLSIGPSNENMAQFDLPSLAKPKITSKY 80
+ G TL + PL + +PG+C GP NE F+ L +P +TS
Sbjct: 327 VNGSSTLAYDPPL----------ELCSPGMCRA---GPCNEKEFAFNASILNEPDLTSLC 373
Query: 81 VKPNVWV 87
V+ + V
Sbjct: 374 VRKELMV 380
>At5g61560 putative protein
Length = 815
Score = 26.6 bits (57), Expect = 9.1
Identities = 22/76 (28%), Positives = 34/76 (43%), Gaps = 8/76 (10%)
Query: 95 RVGPNVWIRNPS-EHNQHNRVGASGKDTNNLASSTGEQGALSVG-------TSTKRLSPN 146
+VG N+ +N H+ HNR G+ D + L + G S G S+ R S
Sbjct: 234 KVGTNIGKQNNEPHHHHHNRAGSLDVDESKLLNQKGFYRTSSSGIGYGGSDISSWRSSQM 293
Query: 147 VELYQNATFSLTQNSS 162
E ++T+S +SS
Sbjct: 294 EEASSSSTYSDPTSSS 309
>At3g23280 unknown protein
Length = 462
Score = 26.6 bits (57), Expect = 9.1
Identities = 22/93 (23%), Positives = 42/93 (44%), Gaps = 1/93 (1%)
Query: 104 NPSEHNQHNRVGASGKDTNNLASSTGEQGALSVGTSTKRLSPNVELYQNATFSLTQNSSL 163
+PS N H+ AS + +++ A + G ++ + +E +A + +
Sbjct: 311 DPSNLNHHSIGQASSSSGPSSSTAPPSGKASAFGFNSHGIGIVLESSPSAPPLTDDDIAT 370
Query: 164 IENTVISPPASD-TDADLPEAPLAPHNEEGRRE 195
+++ I P+ D T DLP A P + EG R+
Sbjct: 371 VDDGPIHYPSIDSTPVDLPSAASLPASTEGERK 403
>At3g21215 unknown protein
Length = 339
Score = 26.6 bits (57), Expect = 9.1
Identities = 15/41 (36%), Positives = 23/41 (55%)
Query: 42 IQSIEAPGVCNTLSIGPSNENMAQFDLPSLAKPKITSKYVK 82
+Q+I+ CNTL IG EN+ + +L SL + K +K
Sbjct: 223 VQNIKDNPPCNTLFIGNLGENINEEELRSLLSAQPGFKQMK 263
>At2g29240 hypothetical protein
Length = 992
Score = 26.6 bits (57), Expect = 9.1
Identities = 18/70 (25%), Positives = 30/70 (42%), Gaps = 2/70 (2%)
Query: 112 NRVGASGKDTNNLASSTGEQGALSVGTSTKRLSPNVELYQNATFSLTQNSSLIENTVISP 171
N+VG DT + G +G+ST +++ + S + S +N ++
Sbjct: 550 NKVGGVVTDTTE--DESARDGIHDIGSSTLPAKELDDMFAEKSVSGDKTVSHQDNILVDA 607
Query: 172 PASDTDADLP 181
PAS T LP
Sbjct: 608 PASHTQCLLP 617
>At1g32750 unknown protein
Length = 1919
Score = 26.6 bits (57), Expect = 9.1
Identities = 18/66 (27%), Positives = 32/66 (48%), Gaps = 2/66 (3%)
Query: 98 PNVWIRNPSEHNQHNR--VGASGKDTNNLASSTGEQGALSVGTSTKRLSPNVELYQNATF 155
P++ IR P+E Q + V K+ N+ S+ E+ TKR++ + +F
Sbjct: 1607 PHLVIRPPTEREQPQKKLVIKRSKEMNDHDMSSLEESPRFESRKTKRMAELAGFQRQQSF 1666
Query: 156 SLTQNS 161
L++NS
Sbjct: 1667 RLSENS 1672
>At1g31460 unknown protein
Length = 301
Score = 26.6 bits (57), Expect = 9.1
Identities = 19/80 (23%), Positives = 34/80 (41%), Gaps = 10/80 (12%)
Query: 103 RNPSEHNQHNRVGASGKDTNNLASSTGEQGALSVGTSTKRLSPNVELYQNATFSLTQNSS 162
RNP + V S +D++NL+ + S S EL N + + + +
Sbjct: 94 RNPGKKT--GSVSLSDEDSSNLSRKKRDLDLKS--------SSQAELVSNGSGDFSDSGN 143
Query: 163 LIENTVISPPASDTDADLPE 182
+ E+ ++SP T LP+
Sbjct: 144 ISESKILSPVKQQTKKKLPK 163
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.310 0.130 0.376
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,623,690
Number of Sequences: 26719
Number of extensions: 189764
Number of successful extensions: 384
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 11
Number of HSP's that attempted gapping in prelim test: 379
Number of HSP's gapped (non-prelim): 15
length of query: 196
length of database: 11,318,596
effective HSP length: 94
effective length of query: 102
effective length of database: 8,807,010
effective search space: 898315020
effective search space used: 898315020
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0016.10