Lotus
BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0014.22
         (284 letters)

Database: ara_mips 
           26,719 sequences; 11,318,596 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

At1g30920 F17F8.21                                                     30  1.5
At2g36490 hypothetical protein                                         29  3.3
At4g10780 putative disease resistance protein                          28  4.3
At1g05020 clathrin like protein                                        28  4.3
At4g02480                                                              28  5.6
At3g60140 beta-glucosidase                                             28  5.6
At1g55350 putative n-calpain-1 large subunit (At1g55350)               28  5.6
At1g08060 storage protein, putative                                    28  5.6
At5g08630 unknown protein                                              28  7.3
At4g01860 unknown protein                                              28  7.3
At5g36300 putative protein                                             27  9.6
At1g70985 unknown protein                                              27  9.6

>At1g30920 F17F8.21
          Length = 1201

 Score = 30.0 bits (66), Expect = 1.5
 Identities = 17/61 (27%), Positives = 27/61 (43%), Gaps = 6/61 (9%)

Query: 162 DETNKALYTTYKPGKSDYKVVDLHPKLRIWHKILLHCINQRPKGSSPDYINFCQKAMLFF 221
           D+  K L+  Y  G  D+K++ L  +   W KI  HC    P    P     C   +L++
Sbjct: 142 DKQFKVLFMAYPSGPDDHKILTLGTRRMRWRKI--HC----PLTHDPFCEGICINGVLYY 195

Query: 222 I 222
           +
Sbjct: 196 L 196


>At2g36490 hypothetical protein
          Length = 1207

 Score = 28.9 bits (63), Expect = 3.3
 Identities = 13/33 (39%), Positives = 18/33 (54%)

Query: 66  WRFNLDNIAANGYDLRPEVQVQGWEEYFNRLRG 98
           W+  L+NI + G D   E + + WEE  N  RG
Sbjct: 511 WKLLLENINSEGVDGSDEQKAKWWEEERNVFRG 543


>At4g10780 putative disease resistance protein
          Length = 892

 Score = 28.5 bits (62), Expect = 4.3
 Identities = 16/60 (26%), Positives = 28/60 (46%), Gaps = 6/60 (10%)

Query: 85  QVQGWEEYFNRLRGPIYDKL------VKEFWKHADCDDTQVVSYIAGRRIIIIEKSIVNL 138
           QVQ W +    +R   YD L      ++    +++C      SY  G+R+ ++ K + NL
Sbjct: 70  QVQVWLKRVEIIRNQFYDLLSARNIEIQRLCFYSNCSTNLSSSYTYGQRVFLMIKEVENL 129


>At1g05020 clathrin like protein
          Length = 653

 Score = 28.5 bits (62), Expect = 4.3
 Identities = 15/42 (35%), Positives = 21/42 (49%)

Query: 166 KALYTTYKPGKSDYKVVDLHPKLRIWHKILLHCINQRPKGSS 207
           KA   +++P   D K V L  K+  W K+L   I  RP G +
Sbjct: 189 KAGIKSHEPAVRDMKPVMLLDKITYWQKLLDRAIATRPTGDA 230


>At4g02480
          Length = 731

 Score = 28.1 bits (61), Expect = 5.6
 Identities = 19/51 (37%), Positives = 26/51 (50%), Gaps = 6/51 (11%)

Query: 72  NIAANGYDLRPEVQVQGWEEYFNRLRGPIYDKL-----VKEFWKHADCDDT 117
           N+ A GY++RP V + G    F+ +RG I   L     VKEF +  D   T
Sbjct: 386 NVPAAGYEIRPIVHLLGESSSFD-IRGSISRLLDERREVKEFLREFDLSST 435


>At3g60140 beta-glucosidase
          Length = 577

 Score = 28.1 bits (61), Expect = 5.6
 Identities = 22/103 (21%), Positives = 41/103 (39%), Gaps = 9/103 (8%)

Query: 133 KSIVNLLGAHTGTGYRFQLTESKVKP--RTKDETNKALYTTYKPGKSDYKVVDLHPKLRI 190
           K  + L+       +RF ++ S++ P  + KD  NK     YK    +    D+ P + +
Sbjct: 87  KDDIKLMKELNMDAFRFSISWSRLIPSGKLKDGVNKEGVQFYKDLIDELLANDIQPSMTL 146

Query: 191 WHKILLHCINQRPKGSSPDYINFCQKAMLFFIQDKRKICLPFF 233
           +H          P+    +Y  F    ++   +D  +IC   F
Sbjct: 147 YHW-------DHPQSLEDEYGGFLSPKIVEDFRDFARICFEEF 182


>At1g55350 putative n-calpain-1 large subunit (At1g55350)
          Length = 2144

 Score = 28.1 bits (61), Expect = 5.6
 Identities = 15/47 (31%), Positives = 23/47 (48%)

Query: 36   QASEKMIPIAERGCAVHCVYAPEELQVLAEWRFNLDNIAANGYDLRP 82
            Q +++  P  +R   V     P +LQV++EW      +  NG D RP
Sbjct: 1688 QFTDQEFPPNDRSLFVDTQNPPSKLQVVSEWMRPDSIVKENGSDSRP 1734


>At1g08060 storage protein, putative
          Length = 1892

 Score = 28.1 bits (61), Expect = 5.6
 Identities = 17/38 (44%), Positives = 20/38 (51%), Gaps = 1/38 (2%)

Query: 142 HTGTGYRFQLTESKVKPRTKDETNKALYTTYKPGKSDY 179
           HT  G RF L E  V  ++KD  N  +   YK GK DY
Sbjct: 120 HTLNGKRFVLEEHMVH-QSKDGRNAVVAFFYKLGKPDY 156


>At5g08630 unknown protein
          Length = 723

 Score = 27.7 bits (60), Expect = 7.3
 Identities = 13/37 (35%), Positives = 24/37 (64%)

Query: 131 IEKSIVNLLGAHTGTGYRFQLTESKVKPRTKDETNKA 167
           IEKS   L+G      ++ Q+T++K++P+ ++E  KA
Sbjct: 519 IEKSEKRLIGNVYLRKHKRQMTDTKIEPKEEEEDEKA 555


>At4g01860 unknown protein
          Length = 1308

 Score = 27.7 bits (60), Expect = 7.3
 Identities = 33/155 (21%), Positives = 59/155 (37%), Gaps = 30/155 (19%)

Query: 108  FWKHADCDDTQVVSYIAGRRIIIIEKSIVNLLGAHTGTGY----RFQLTESKVKPRTKDE 163
            FW    C    V +++     I IEK I   L   TG G     +++L  SK+  R ++ 
Sbjct: 1048 FWDVTKC----VEAFVKQVSSIHIEKYIDCQLRPRTGRGSQGGRKWKLLGSKISKRAQEN 1103

Query: 164  TNKALYTTYKPGKSDYKVVDLHPK----------------------LRIWHKILLHCINQ 201
            +N       +   S  ++ + HP+                      ++  H+  ++C++ 
Sbjct: 1104 SNSVGEAAEEDPASSLELTNDHPQENGKNEDADSLPETSEIKTSHVVKNAHQSGVNCLHV 1163

Query: 202  RPKGSSPDYINFCQKAMLFFIQDKRKICLPFFLFS 236
                SSP Y N     ++    D+   CL F + S
Sbjct: 1164 SRSNSSPSYGNGLMFNVISGGDDQALHCLSFNILS 1198


>At5g36300 putative protein
          Length = 326

 Score = 27.3 bits (59), Expect = 9.6
 Identities = 15/60 (25%), Positives = 29/60 (48%), Gaps = 3/60 (5%)

Query: 188 LRIWHKI---LLHCINQRPKGSSPDYINFCQKAMLFFIQDKRKICLPFFLFSYLKECIRK 244
           L I+H I   +L+C++   +G  PD + +      F    K ++   F+L + + E  R+
Sbjct: 264 LSIYHHIKETMLNCLSLSSEGIHPDVVTYSTLMKAFTRAKKYEMVCSFYLVTLVPEIFRE 323


>At1g70985 unknown protein
          Length = 135

 Score = 27.3 bits (59), Expect = 9.6
 Identities = 14/42 (33%), Positives = 24/42 (56%)

Query: 230 LPFFLFSYLKECIRKSITTASIKSAIKYIPFGRLLSDLFIES 271
           LP+F F Y K   +   +++S+KS +K +    LL+ L + S
Sbjct: 93  LPYFPFYYRKPPHQTDQSSSSMKSTVKIVTVANLLAVLMLFS 134


  Database: ara_mips
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 2,978,382
  Number of sequences in database:  6832
  
  Database: /data/blast2/ara_mips_chr2
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 1,737,135
  Number of sequences in database:  4184
  
  Database: /data/blast2/ara_mips_chr3
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 2,236,886
  Number of sequences in database:  5377
  
  Database: /data/blast2/ara_mips_chr4
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 1,748,816
  Number of sequences in database:  4030
  
  Database: /data/blast2/ara_mips_chr5
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 2,569,679
  Number of sequences in database:  6098
  
  Database: /data/blast2/ara_mips_chl
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 25,951
  Number of sequences in database:  85
  
  Database: /data/blast2/ara_mips_mit
    Posted date:  Jul 15, 2004 10:29 AM
  Number of letters in database: 21,747
  Number of sequences in database:  113
  
Lambda     K      H
   0.322    0.139    0.427 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,424,364
Number of Sequences: 26719
Number of extensions: 273377
Number of successful extensions: 536
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 530
Number of HSP's gapped (non-prelim): 12
length of query: 284
length of database: 11,318,596
effective HSP length: 98
effective length of query: 186
effective length of database: 8,700,134
effective search space: 1618224924
effective search space used: 1618224924
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 59 (27.3 bits)


Lotus: description of TM0014.22