BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0012a.1
(263 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g49890 unknown protein 35 0.031
At5g24880 glutamic acid-rich protein 32 0.27
At1g15280 unknown protein 32 0.27
At1g42080 hypothetical protein 32 0.35
At5g18590 RanGAP1 interacting protein 32 0.45
At3g28790 unknown protein 32 0.45
At2g35670 fertilization-independent seed 2 protein (FIS2) 32 0.45
At1g34090 putative protein 32 0.45
At3g23900 unknown protein 31 0.59
At1g21510 hypothetical protein 31 0.59
At1g17220 putative translation initiation factor IF2 31 0.77
At3g51540 unknown protein 30 1.0
At5g65620 oligopeptidase A 30 1.3
At2g40070 En/Spm-like transposon protein 30 1.3
At2g29970 unknown protein 30 1.3
At2g17150 unknown protein 30 1.3
At4g39610 unknown protein 30 1.7
At3g20250 RNA-binding protein, putative 30 1.7
At3g08670 hypothetical protein 30 1.7
At1g70620 unknown protein 30 1.7
>At1g49890 unknown protein
Length = 659
Score = 35.4 bits (80), Expect = 0.031
Identities = 30/116 (25%), Positives = 51/116 (43%), Gaps = 17/116 (14%)
Query: 119 PQRRQERASNNQGRRTMLPSEGTRTSLPKLPSTQLKSSPSPAKTKASSDHRSLTTTLGKR 178
P+ R + +NN G+R R ++PS L SPS + + ++ + TTT
Sbjct: 15 PRDRPQSLTNNGGQR--------RPRGKQVPSRYLSPSPSHSVSSTTTTTTTTTTTTSSS 66
Query: 179 QSSKTLLPSSQVIRTSK-----TRSLEKISGNKVATDILSVSTPPRQHPEIRHSSP 229
SS SS ++RTSK + L + + N + I + S P++ + P
Sbjct: 67 SSSS----SSAILRTSKRYPSPSPLLSRSTTNSASNSIKTPSLLPKRSQSVDRRRP 118
>At5g24880 glutamic acid-rich protein
Length = 443
Score = 32.3 bits (72), Expect = 0.27
Identities = 33/138 (23%), Positives = 54/138 (38%), Gaps = 10/138 (7%)
Query: 125 RASNNQGRRTMLPSEGTRTSLPKLPSTQLKSSPSPAKTKASSDHRSLTTTLGKRQSSKTL 184
R+S+ G R + T S P P KS + + T + S K+ S K +
Sbjct: 131 RSSSFHGSRNVPKGGNTAKSPPVAPK---KSGLNSSSTSSKSKKEGSENVRIKKASDKEI 187
Query: 185 LPSSQVIRTSKTRSLEKISGNKVATDILSVSTPPRQHPEIRHSSPGERTRTLCSPCFPTT 244
S + +++ E+I KV +D L VS + P+ + + +
Sbjct: 188 ALDSASMSSAQEDHQEEIL--KVESDHLQVSDHDIEEPKYEKEEKEVQEKVV-----QAN 240
Query: 245 TSRQEKVEGKDPTPFLNP 262
S +EK E PTP +P
Sbjct: 241 ESVEEKAESSGPTPVASP 258
>At1g15280 unknown protein
Length = 584
Score = 32.3 bits (72), Expect = 0.27
Identities = 25/96 (26%), Positives = 45/96 (46%), Gaps = 7/96 (7%)
Query: 119 PQRRQERASNNQGRRTMLPSEGTRTSLPKLPSTQLKSSP-SPAKTKASSDHRSLTTTLGK 177
P R + +A + Q ++ + S G++ S K+P + SP +PAKT+ H +
Sbjct: 220 PLRNENQAHSEQSKQ-LRNSNGSQNSREKMPHLDSRRSPTAPAKTENQGAHAKKNVAVSS 278
Query: 178 RQSSK----TLLPSSQVIRTSKTRSLEKISGNKVAT 209
S+ PSS V+ + S+E++ N+ T
Sbjct: 279 LSSASPPFYPSAPSSNVVHGIQV-SMERLHTNESVT 313
>At1g42080 hypothetical protein
Length = 151
Score = 32.0 bits (71), Expect = 0.35
Identities = 21/77 (27%), Positives = 37/77 (47%), Gaps = 2/77 (2%)
Query: 51 DEEAKELTKAEPSVNNKESSNSVVPGINEEDPPTSKTVSLALIFLGLPRERELLKAHHKR 110
DE A+ L++ P ++E ++ +V +N P+ LI L + L A
Sbjct: 20 DEPAEGLSERLPEGFSEEPADELVDKLNLTKEPSDVLEDFLLIIFTLMKTTRNLGA--AE 77
Query: 111 TCHIRYIHPQRRQERAS 127
HI + HP+RR+++ S
Sbjct: 78 MTHILFTHPKRREKKLS 94
>At5g18590 RanGAP1 interacting protein
Length = 708
Score = 31.6 bits (70), Expect = 0.45
Identities = 16/56 (28%), Positives = 28/56 (49%)
Query: 156 SPSPAKTKASSDHRSLTTTLGKRQSSKTLLPSSQVIRTSKTRSLEKISGNKVATDI 211
S SP + +S GKR SS +L S + ++ S R ++ ++ K+A+ I
Sbjct: 381 SESPTHPQTTSKKNPGRLLFGKRSSSSAVLTSDESVKASSQRLIDSVARQKLASAI 436
>At3g28790 unknown protein
Length = 608
Score = 31.6 bits (70), Expect = 0.45
Identities = 23/82 (28%), Positives = 37/82 (45%), Gaps = 2/82 (2%)
Query: 137 PSEGTRTSLPKLPSTQLKSSPSPAKTKA--SSDHRSLTTTLGKRQSSKTLLPSSQVIRTS 194
P+ T T PST S+P+P+ A +S+ S + ++ K +SK+ S+ S
Sbjct: 291 PTPSTPTPSTPTPSTPTPSTPAPSTPAAGKTSEKGSESASMKKESNSKSESESAASGSVS 350
Query: 195 KTRSLEKISGNKVATDILSVST 216
KT+ K S D S+
Sbjct: 351 KTKETNKGSSGDTYKDTTGTSS 372
>At2g35670 fertilization-independent seed 2 protein (FIS2)
Length = 488
Score = 31.6 bits (70), Expect = 0.45
Identities = 37/146 (25%), Positives = 56/146 (38%), Gaps = 10/146 (6%)
Query: 120 QRRQERASNNQGRRTMLPSEGTRTSLPK---LPSTQLK----SSPSPAKTKASSDHRSLT 172
QRRQ SNN + +L E LP+ ST + SSP A + T
Sbjct: 87 QRRQRDDSNNVKKLNVLLMELDLDDLPRGTENDSTHVNDDNVSSPPRAHSSEKISDILTT 146
Query: 173 TTLGKRQSSKTLLP---SSQVIRTSKTRSLEKISGNKVATDILSVSTPPRQHPEIRHSSP 229
T L +SS+ +P V + S + + + D VS+PPR H ++ S
Sbjct: 147 TQLAIAESSEPKVPHVNDGNVSSPPRAHSSAEKNESTHVNDDDDVSSPPRAHSLEKNEST 206
Query: 230 GERTRTLCSPCFPTTTSRQEKVEGKD 255
+ SP ++ + E D
Sbjct: 207 HVNEDNISSPPKAHSSKKNESTHMND 232
>At1g34090 putative protein
Length = 761
Score = 31.6 bits (70), Expect = 0.45
Identities = 26/110 (23%), Positives = 47/110 (42%), Gaps = 9/110 (8%)
Query: 130 QGRRTMLPSEGTRTSLPKLPSTQLKSSPSPAKTKASSDHRSLTTTLGKRQSSKTLLPSSQ 189
Q T T TS+P P+T +SS +T SS + + S + +P+
Sbjct: 88 QASSTSSEKSKTPTSIPSTPTTPQRSSTESTETPMSS------PVITQPSPSASSIPTMH 141
Query: 190 VIRTSKTRSLE-KISGNKVATDILSV--STPPRQHPEIRHSSPGERTRTL 236
+S + S + ++ NK TD++ + ++P P I + T T+
Sbjct: 142 AATSSASTSQQSSVASNKSTTDVVQIQEASPKSTAPCIEAPVEKQPTHTM 191
>At3g23900 unknown protein
Length = 989
Score = 31.2 bits (69), Expect = 0.59
Identities = 29/115 (25%), Positives = 52/115 (45%), Gaps = 17/115 (14%)
Query: 120 QRRQERASNNQGRRTMLPSEGTRTSLPKLPSTQLKSSPSPAKTKASSDHRSLTTTLGKRQ 179
+RR+ R+ + +G+R+ +R K S + SPS ++ K D RS +++
Sbjct: 787 RRRRSRSKSVEGKRSYNKETRSRDKKSKRRSGRRSRSPS-SEGKQGRDIRSSPGYSDEKK 845
Query: 180 SSKTLLPSSQVIRTSKTRSLEKISGNKVATDILSVSTPPRQHPEIRHSSPGERTR 234
S R S++RS+EK + ++ ++H +R SSPG R
Sbjct: 846 SRHK--------RHSRSRSIEKKNSSR--------DKRSKRHERLRSSSPGRDKR 884
>At1g21510 hypothetical protein
Length = 323
Score = 31.2 bits (69), Expect = 0.59
Identities = 40/196 (20%), Positives = 74/196 (37%), Gaps = 13/196 (6%)
Query: 19 PSYTKPTTLPRSHAFFARVKKRPLLTVP-KSGTDEEAKELTKAEPSVNNKESSNSVVPGI 77
PS P LP + F + +P K +L P + S++S+
Sbjct: 38 PSRVSPLNLPEKNRFHPIPVPNKFINIPGKVIPPPSPVKLASFSPIQPSTTSNSSLSSSN 97
Query: 78 NEEDPPTSKTVSLALIFLGLPRERELLKAHHKRTCHIRYIHPQRRQERASNNQGRR---- 133
+ +S +VS F + L+A ++ H+ I+ R + R
Sbjct: 98 SSLSTASSVSVSKERSF-----SNDFLRACYQENSHVARINSLREASLSMKTTKPRYPSR 152
Query: 134 ---TMLPSEGTRTSLPKLPSTQLKSSPSPAKTKASSDHRSLTTTLGKRQSSKTLLPSSQV 190
++PS + T ++ S+ S + S +HRSLT RQ + ++ SS
Sbjct: 153 FDSPVIPSRNSTTPNRANEDSKRGSNCSKRTRELSPNHRSLTRQKSFRQDQERVVISSSS 212
Query: 191 IRTSKTRSLEKISGNK 206
+K + L+ S ++
Sbjct: 213 NSLTKGKYLKSPSPSR 228
>At1g17220 putative translation initiation factor IF2
Length = 1026
Score = 30.8 bits (68), Expect = 0.77
Identities = 44/197 (22%), Positives = 79/197 (39%), Gaps = 13/197 (6%)
Query: 39 KRPLLTVPKSGTDEEAKELTKAEPSVNNKESSNSVVPGINEEDPPTSKTVSLALIFL--- 95
K L +PK G E + + ++PS N+ S N N D T KT ++ ++
Sbjct: 157 KAEKLEIPKPGNKEGGEAVKPSQPSANSSNSRNGSY--ANASDGGTRKTKTMKSVWRKGD 214
Query: 96 GLPRERELLKAHHKRTCHIRYIHPQRRQERASNNQGRRTMLPSEGT-RTSLPKLPSTQLK 154
+ ++++K K P+ R+E N + + P + R P P L+
Sbjct: 215 AVAAVQKVVKESPKIFNRGVQTEPRTREEGEVNAKAGTPLAPPQPPFRPQPPVRPQPMLQ 274
Query: 155 SSPSPAKTKASSDHRSLTTTLGKRQSSKTLLPSSQVIRTSKTRSLEKISGNKVATDILSV 214
P A S + LG ++K L+ S +V + K++ + I +K A+ V
Sbjct: 275 GKPMVAPPVKKS---PILKDLG--MAAKPLV-SEEVDSSVKSKERKPILVDKFASKKKGV 328
Query: 215 STPPRQHPEIRHSSPGE 231
P + + PG+
Sbjct: 329 D-PAASQAVLAPTKPGK 344
>At3g51540 unknown protein
Length = 438
Score = 30.4 bits (67), Expect = 1.0
Identities = 40/135 (29%), Positives = 57/135 (41%), Gaps = 16/135 (11%)
Query: 138 SEGTRTSLPKLPST--QLKSSPSPAKTKASSDHRSLTTTLGKRQSSKTLLPSSQVIRTSK 195
S TR+ PS ++SS P KT + S + + KR S++L PSS+ S
Sbjct: 111 SRPTRSGSTIRPSNIPTIRSSSVPKKTTTTQIQASASVSSPKRTVSRSLTPSSRK-TPSP 169
Query: 196 TRSLEKISGN-------KVATDI-LSVSTPPRQHPEIRHSSPGERTRTLCSPCFPTTTSR 247
T + +IS K A D S S PR P+I ++ RT S + TSR
Sbjct: 170 TSTPSRISTTTSTTPSFKTAGDAQRSRSLTPRAKPQIA-ANASSRTNVRSS----SVTSR 224
Query: 248 QEKVEGKDPTPFLNP 262
++ TP P
Sbjct: 225 PDRSTTVSATPRTPP 239
>At5g65620 oligopeptidase A
Length = 791
Score = 30.0 bits (66), Expect = 1.3
Identities = 32/117 (27%), Positives = 47/117 (39%), Gaps = 12/117 (10%)
Query: 125 RASNNQGRRTMLPSEGTRTSLPKLPSTQLKSSPSPAKTKA---------SSDHRSLTTTL 175
RAS N RR+ P + +S PST KS P P + + S+ SL+++
Sbjct: 9 RASLNLLRRSPKPKYFSSSSCHFRPSTFRKSYPCPIWSSSFSFCLPPPRSTTSTSLSSSS 68
Query: 176 GKRQSSKTLLPSSQVIRTSKTRSLEKISGNKVATDILSVSTPPRQHPEIRHSSPGER 232
+ SS + S+ S E +S N + D PP + H PG R
Sbjct: 69 FRPFSSPPSMSSAAAAAVESVVSDETLSSNPLLQDF---DFPPFDSVDASHVRPGIR 122
>At2g40070 En/Spm-like transposon protein
Length = 510
Score = 30.0 bits (66), Expect = 1.3
Identities = 25/95 (26%), Positives = 40/95 (41%), Gaps = 11/95 (11%)
Query: 4 SSRTFSRPCIA-SMSLPSYTKPTTLPRSHAFFARVKKRPLLTVPKSGTDEEAKELTK--- 59
SS SRP + S ++ + PT P + A A P ++ K + AK +
Sbjct: 268 SSTPTSRPTLPPSKTISRSSTPTRRPIASASAATTTANPTISQIKPSSPAPAKPMPTPSK 327
Query: 60 -------AEPSVNNKESSNSVVPGINEEDPPTSKT 87
A P+V ++ S +PG + E PP +T
Sbjct: 328 NPALSRAASPTVRSRPWKPSDMPGFSLETPPNLRT 362
>At2g29970 unknown protein
Length = 1002
Score = 30.0 bits (66), Expect = 1.3
Identities = 18/65 (27%), Positives = 27/65 (40%), Gaps = 6/65 (9%)
Query: 63 SVNNKESSNSVVPGINEEDPPTSKTVSLALIFLGLPRERELLKAHHKRTCHIRYIHPQRR 122
S++ SS S EEDPP S ++ A+ + + + H T H+ IH
Sbjct: 82 SLDRLPSSKSTPTTTVEEDPPVSNSLMAAI------KRSQATQRRHPETYHLHQIHGNNN 135
Query: 123 QERAS 127
E S
Sbjct: 136 TETTS 140
>At2g17150 unknown protein
Length = 909
Score = 30.0 bits (66), Expect = 1.3
Identities = 29/109 (26%), Positives = 47/109 (42%), Gaps = 4/109 (3%)
Query: 130 QGRRTMLPSEGTRTSLPKLPSTQLKSSPSPAKTKASSDHRSLTTTLGKRQSSKTLLPSSQ 189
QG + + + TS P+L S + S+ K+ H + T G PSS
Sbjct: 671 QGAQGSIQLDSFYTSFPELNSPNMSSNGPSLKSNEQPSHLNAQTDNGIMAEENPRSPSSS 730
Query: 190 VIRTSKTRSLEKISGN-KVATDILSVSTPPRQHPEIR-HSSPGERTRTL 236
++S + + + +GN VA D +V R H E + H+ E T+ L
Sbjct: 731 CSKSSGSSNNNENTGNILVAEDADAVL--KRAHSEAQLHNVNQEETKCL 777
>At4g39610 unknown protein
Length = 264
Score = 29.6 bits (65), Expect = 1.7
Identities = 18/50 (36%), Positives = 26/50 (52%), Gaps = 1/50 (2%)
Query: 187 SSQVIRTSKTRSLEKISGNKVATDILSVSTPPRQHPEIR-HSSPGERTRT 235
SS + TS + S I + S +TPPRQHP ++ SS ++ RT
Sbjct: 4 SSNPMATSSSSSSSSIPPSSPTPSPTSTTTPPRQHPLLQPPSSKKKKNRT 53
>At3g20250 RNA-binding protein, putative
Length = 955
Score = 29.6 bits (65), Expect = 1.7
Identities = 21/90 (23%), Positives = 39/90 (43%), Gaps = 1/90 (1%)
Query: 128 NNQGRRTMLPSEGTRTSLPKLPSTQLKSSPSPAKTKASSDHRSLTTTLGKRQSSKTLLPS 187
NNQ T + G R+SL + P +S + +S + G+R+S ++
Sbjct: 141 NNQSPTTSWDNMGIRSSLHSSRTALSTHREEPEDEASSGEQQSYASLAGRRKSIADMIQE 200
Query: 188 SQVIRTSKTRSLEKISGNK-VATDILSVST 216
+ S +GN+ +A DI ++S+
Sbjct: 201 DFPLTLSSVFKRPHSAGNRPIAQDIHAISS 230
>At3g08670 hypothetical protein
Length = 567
Score = 29.6 bits (65), Expect = 1.7
Identities = 31/101 (30%), Positives = 44/101 (42%), Gaps = 7/101 (6%)
Query: 146 PKLPSTQLKSSPSPAKTKASSDHRSLTTTLGKRQSSKTLLPSSQVIRTSKTRSLEKISGN 205
PK+ S+ SS S A + S S + +SS PS I TS+ S +
Sbjct: 120 PKIASSARASSASKASRLSVSQSESGYHSSRPARSSSVTRPS---ISTSQYSSF---TSG 173
Query: 206 KVATDILSVSTPPRQHPEIRHSSPGERTRTLCSPCFPTTTS 246
+ + IL+ S+ IR SSP R+ + P PT TS
Sbjct: 174 RSPSSILNTSSASVSS-YIRPSSPSSRSSSSARPSTPTRTS 213
>At1g70620 unknown protein
Length = 897
Score = 29.6 bits (65), Expect = 1.7
Identities = 21/63 (33%), Positives = 33/63 (52%), Gaps = 2/63 (3%)
Query: 121 RRQERASNNQGRRTMLPSEGTRTSLPKLPSTQLKSSPSPAKT--KASSDHRSLTTTLGKR 178
RR E +S ++ RR+ +E + S K S + SPSP ++ K SS ++ KR
Sbjct: 776 RRAENSSKDKRRRSPTSNESSDDSKRKSRSRRRSVSPSPVRSRRKRSSPSSDESSDDSKR 835
Query: 179 QSS 181
+SS
Sbjct: 836 KSS 838
Score = 27.7 bits (60), Expect = 6.6
Identities = 26/130 (20%), Positives = 55/130 (42%), Gaps = 2/130 (1%)
Query: 51 DEEAKELTKAEPSVNNKESSNSVVPGINEEDPPTSKTVSLALIFLGLPRERELLKAHHKR 110
+ + K K + +NK+S++ V + + +T S +E+E ++ H+R
Sbjct: 718 ENDLKSAEKVKGVESNKKSTDPHVKKDSRDVERPHRTNSKEDRGKRKEKEKEEERSRHRR 777
Query: 111 TCHIRYIHPQRRQERASNNQGRRTMLPSEGTRTSLPKLPSTQLKSSPSPAKTKASSDHRS 170
+ +RR+ SN + S R S+ P + SP+ ++S D +
Sbjct: 778 AENSS--KDKRRRSPTSNESSDDSKRKSRSRRRSVSPSPVRSRRKRSSPSSDESSDDSKR 835
Query: 171 LTTTLGKRQS 180
+++ K +S
Sbjct: 836 KSSSKRKNRS 845
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.310 0.125 0.354
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,126,788
Number of Sequences: 26719
Number of extensions: 259819
Number of successful extensions: 927
Number of sequences better than 10.0: 62
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 53
Number of HSP's that attempted gapping in prelim test: 896
Number of HSP's gapped (non-prelim): 84
length of query: 263
length of database: 11,318,596
effective HSP length: 97
effective length of query: 166
effective length of database: 8,726,853
effective search space: 1448657598
effective search space used: 1448657598
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0012a.1