BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0010.3
(172 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g40820 AtRAD3 (dbj|BAA92828.1) 124 2e-29
At2g22910 amino acid acetyltransferase like protein 33 0.100
At1g07620 GTP-binding protein like 30 0.65
At3g48860 putative protein 30 0.84
At1g73870 unknown protein 29 1.4
At5g07670 unknown protein 28 1.9
At2g06890 putative retroelement integrase 28 1.9
At5g55470 sodium proton exchanger 28 2.5
At5g52140 unknown protein 28 2.5
At5g53810 caffeic acid 3-O-methyltransferase-like protein 27 5.5
At1g13420 unknown protein 27 5.5
At5g49310 importin alpha 27 7.1
At5g44600 unknown protein 26 9.3
At4g21660 spliceosome associated protein - like 26 9.3
At4g17695 GARP-like putative transcription factor KANADI3 (KAN3) 26 9.3
At2g39240 unknown protein 26 9.3
>At5g40820 AtRAD3 (dbj|BAA92828.1)
Length = 2810
Score = 124 bits (312), Expect = 2e-29
Identities = 76/141 (53%), Positives = 94/141 (65%), Gaps = 10/141 (7%)
Query: 40 NLSSLP----ERIAASSSTLTPN--NPSGE--ALEVRFCTVLPNLLHAYVVPSSSRNNDR 91
NLSSL ER+AAS+ST N + SG+ ALE+RF V+PNLL+ YVVPS N R
Sbjct: 7 NLSSLVHELRERVAASASTPANNLRHSSGDEDALEIRFRAVIPNLLNTYVVPSLG--NGR 64
Query: 92 EVIAVVKLISHTARNFPGVFFLGKPSAVLPVLADILPSYADPLFRSRHRVFFEALGSLLS 151
EV AV+KL+ HTARN PGVF+ G PSA+LPV+A I+P +A+P F H V E +GSLL
Sbjct: 65 EVTAVLKLVGHTARNIPGVFYHGTPSAILPVIARIIPFFAEPEFVPGHGVLLETVGSLLM 124
Query: 152 LFRSGARDASTHFFLDPMLLI 172
L RS +R A FF D + I
Sbjct: 125 LLRSNSRKAYRIFFHDALQAI 145
>At2g22910 amino acid acetyltransferase like protein
Length = 609
Score = 32.7 bits (73), Expect = 0.100
Identities = 16/49 (32%), Positives = 24/49 (48%)
Query: 17 TLVRVREEEEEEEEAYFVSMAKANLSSLPERIAASSSTLTPNNPSGEAL 65
TLVR R ++ E Y ++ +SS PE + + TPNN G +
Sbjct: 308 TLVRKRAQQSEIAANYVKAVGDGGISSFPEPLGYNGMVTTPNNHIGRPI 356
>At1g07620 GTP-binding protein like
Length = 1016
Score = 30.0 bits (66), Expect = 0.65
Identities = 25/103 (24%), Positives = 43/103 (41%), Gaps = 16/103 (15%)
Query: 19 VRVREEEEEEEEAYFVSMAKANLSSLPERIAASSSTLTPNNPSGEALEVRFCTVLPNLLH 78
+ + + EEE+E+AYF + K S N + E + P+LL+
Sbjct: 354 LEIYKHEEEDEDAYFCYVKK----------VLKVSGFLENKDNEEKWYSEEQPLNPSLLY 403
Query: 79 AYVVPSSSRNNDREVI------AVVKLISHTARNFPGVFFLGK 115
+ ND+E++ A+V+ +H+ FP F GK
Sbjct: 404 ELDIQEEETVNDKELLFDLVNEAIVETQNHSQIYFPKTFPYGK 446
>At3g48860 putative protein
Length = 609
Score = 29.6 bits (65), Expect = 0.84
Identities = 39/148 (26%), Positives = 61/148 (40%), Gaps = 16/148 (10%)
Query: 24 EEEEEEEAYFVSMAKANLSSLPERIAASSSTLTPNNPSGEALEVRFCTVLPNLLHAYVVP 83
+E++E+E +S+ A+ S P R A SS + T +N S + + P P
Sbjct: 74 DEDDEDEDEDLSLRFASASLKPARHAPSSISSTGSNSSNGNNNLPAVSFAPRTRSP--SP 131
Query: 84 SSSRNNDREVIAVVKLI----------SHTARNFPGVFFLGKPSAVLPVLADILPSYADP 133
+ RN +V + V+ S T P + + S PV L DP
Sbjct: 132 ALGRNFAEQVPSSVRSASAGRPSMSARSTTPTPIPNLMPPSRVSVKTPVSIPPL----DP 187
Query: 134 LFRSRHRVFFEALGSLLSLFRSGARDAS 161
RSR + FF + S+ S + R+AS
Sbjct: 188 PTRSRDKRFFADVPSVNSKEKGDQREAS 215
>At1g73870 unknown protein
Length = 392
Score = 28.9 bits (63), Expect = 1.4
Identities = 13/29 (44%), Positives = 19/29 (64%)
Query: 3 VKEKELITFTFPHSTLVRVREEEEEEEEA 31
+KE+ + F L RV++E+EEEEEA
Sbjct: 225 IKEENKVGFEINCKDLKRVKDEDEEEEEA 253
>At5g07670 unknown protein
Length = 476
Score = 28.5 bits (62), Expect = 1.9
Identities = 19/52 (36%), Positives = 27/52 (51%), Gaps = 3/52 (5%)
Query: 41 LSSLPERIAASSSTLTPNNPSGEALEVRFCTVLPNLLHAYV---VPSSSRNN 89
LSS P +AA +LTP + L F +LP+L+ V +P+S R N
Sbjct: 33 LSSSPLDLAAKFQSLTPPISKSKTLLPDFTLLLPDLILIRVIQKIPNSQRKN 84
>At2g06890 putative retroelement integrase
Length = 1215
Score = 28.5 bits (62), Expect = 1.9
Identities = 14/40 (35%), Positives = 24/40 (60%)
Query: 122 VLADILPSYADPLFRSRHRVFFEALGSLLSLFRSGARDAS 161
V+ D+L + A P+ RSR ++F +A+ +L+ R D S
Sbjct: 1158 VVEDVLVTPAVPMTRSRAKLFDQAIAGMLNHIRDRPNDLS 1197
>At5g55470 sodium proton exchanger
Length = 529
Score = 28.1 bits (61), Expect = 2.5
Identities = 16/39 (41%), Positives = 21/39 (53%)
Query: 65 LEVRFCTVLPNLLHAYVVPSSSRNNDREVIAVVKLISHT 103
L V FC VL + +Y V SSR R V A++ I+ T
Sbjct: 274 LTVFFCGVLMSHYASYNVTESSRITSRHVFAMLSFIAET 312
>At5g52140 unknown protein
Length = 277
Score = 28.1 bits (61), Expect = 2.5
Identities = 23/89 (25%), Positives = 39/89 (42%), Gaps = 3/89 (3%)
Query: 5 EKELITFTFPHSTLVRVREEEEEEEEAYFVSMAKANLSSLPERIAASSSTLTPNNPSGEA 64
+++L + H L E++ +E + + N S +P+R A SSS + GE
Sbjct: 91 DEKLARYLQEHDELANNTEDDIQETHHHQYHPMQVNRSYIPQRDAPSSSRAIFHYDDGEN 150
Query: 65 LEVRFC-TVLPNLLHAYVVPSSSRNNDRE 92
R+ T P+ + P+ NND E
Sbjct: 151 FSGRYTHTHSPSSISHN--PTDDENNDPE 177
>At5g53810 caffeic acid 3-O-methyltransferase-like protein
Length = 378
Score = 26.9 bits (58), Expect = 5.5
Identities = 13/32 (40%), Positives = 21/32 (65%)
Query: 20 RVREEEEEEEEAYFVSMAKANLSSLPERIAAS 51
RV+EE+E EEEA ++ AN ++ P + A+
Sbjct: 14 RVKEEQEVEEEARLLARRLANAAASPMVLKAA 45
>At1g13420 unknown protein
Length = 331
Score = 26.9 bits (58), Expect = 5.5
Identities = 15/33 (45%), Positives = 19/33 (57%)
Query: 23 EEEEEEEEAYFVSMAKANLSSLPERIAASSSTL 55
+EEEEEEE K+ +SSLP I S + L
Sbjct: 11 KEEEEEEEENQSEETKSLISSLPSDIDCSGTKL 43
>At5g49310 importin alpha
Length = 519
Score = 26.6 bits (57), Expect = 7.1
Identities = 16/71 (22%), Positives = 34/71 (47%), Gaps = 4/71 (5%)
Query: 82 VPSSSRNNDREVIAVVKLISHTARNFPGVFFLGKPSAVLPVLADILPSYADPLFRSRHRV 141
+P + N+ +++++ + T NF F GKPS ++ +LP ++ +V
Sbjct: 201 IPLLHQLNNHATLSILRNATWTLSNF----FRGKPSPPFDLVKHVLPVLKRLVYSDDEQV 256
Query: 142 FFEALGSLLSL 152
+A +L +L
Sbjct: 257 LIDACWALSNL 267
>At5g44600 unknown protein
Length = 348
Score = 26.2 bits (56), Expect = 9.3
Identities = 14/42 (33%), Positives = 24/42 (56%)
Query: 22 REEEEEEEEAYFVSMAKANLSSLPERIAASSSTLTPNNPSGE 63
+EEEEEEEE + V+ ++ + + ASS L ++ G+
Sbjct: 65 KEEEEEEEEYWVVTAVRSRYNEIVIVDTASSRYLLLDSTRGQ 106
>At4g21660 spliceosome associated protein - like
Length = 584
Score = 26.2 bits (56), Expect = 9.3
Identities = 22/64 (34%), Positives = 30/64 (46%), Gaps = 1/64 (1%)
Query: 5 EKELITFTFPHSTLVRVREEEEEEEEAYFVSMAKANLSSLPERIAASSSTLTPNNPSGEA 64
E+E I + L EEEEEEEE M + L E + SST T + +A
Sbjct: 396 EEEPIDKSKHWGDLEEEEEEEEEEEEEQEEEMDEEELEDGTESVDTLSSTPT-GIETPDA 454
Query: 65 LEVR 68
+E+R
Sbjct: 455 IELR 458
>At4g17695 GARP-like putative transcription factor KANADI3 (KAN3)
Length = 322
Score = 26.2 bits (56), Expect = 9.3
Identities = 12/31 (38%), Positives = 17/31 (54%)
Query: 46 ERIAASSSTLTPNNPSGEALEVRFCTVLPNL 76
ER+++ SS+LT P E + F PNL
Sbjct: 290 ERLSSDSSSLTGTRPETETPNLDFTLATPNL 320
>At2g39240 unknown protein
Length = 545
Score = 26.2 bits (56), Expect = 9.3
Identities = 27/95 (28%), Positives = 44/95 (45%), Gaps = 10/95 (10%)
Query: 61 SGEALEVRFCTVLPNLLHAYVVPSSSRNNDREVIAVVKLISHTARNFPGVFFLGKPSAVL 120
SG+ L T++ NL S R D +++ V+ + H A + PS +L
Sbjct: 122 SGKHLSSCLDTLVSNLR------SQPRKLDNKMLEVLSRV-HAALHKISYLVPLAPSILL 174
Query: 121 PVLADILPSYADPLFRSRHRVFFEALGSLLSLFRS 155
P+LA +PS D ++R +V + +LL L S
Sbjct: 175 PILAQSMPSVHD---KARLQVVVPYVKNLLKLENS 206
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.321 0.135 0.380
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,885,609
Number of Sequences: 26719
Number of extensions: 163954
Number of successful extensions: 687
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 671
Number of HSP's gapped (non-prelim): 18
length of query: 172
length of database: 11,318,596
effective HSP length: 92
effective length of query: 80
effective length of database: 8,860,448
effective search space: 708835840
effective search space used: 708835840
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0010.3