BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0010.3.5
(80 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g23930 putative small nuclear ribonucleoprotein E 145 4e-36
At3g11500 putative small nuclear ribonucleoprotein polypeptide G 143 1e-35
At2g03870 putative snRNP splicing factor 58 7e-10
At3g14080 Sm protein, putative 49 6e-07
At1g19120 unknown protein 45 6e-06
At3g59810 U6 snRNA-associated Sm-like protein 41 9e-05
At1g65700 small nuclear ribonucleoprotein, putative 41 1e-04
At2g43810 putative small nuclear ribonucleoprotein polypeptide F 39 4e-04
At1g76860 unknown protein 35 0.007
At4g30220 snRNP Sm protein F - like 34 0.011
At2g18740 small nuclear ribonucleoprotein E like protein 34 0.011
At5g44500 unknown protein 34 0.015
At4g30330 small nuclear ribonucleoprotein homolog 33 0.025
At5g48870 Sm-like protein (SAD1) 33 0.033
At1g21190 putative protein 32 0.043
At4g20440 putative snRNP protein 32 0.057
At3g07590 putative small nuclear ribonucleoprotein (Sm-D1) 27 1.8
At1g03330 unknown protein 27 1.8
At3g56150 PROBABLE EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SU... 26 3.1
At2g45440 putative dihydrodipicolinate synthase 26 3.1
>At2g23930 putative small nuclear ribonucleoprotein E
Length = 80
Score = 145 bits (366), Expect = 4e-36
Identities = 70/80 (87%), Positives = 76/80 (94%)
Query: 1 MSRSGQPPDLKKYMDKNLQIKLNANRMIVGTLRGYDQFMNMVVDNTVEVNGNEKNDIGMV 60
MSRSGQPPDLKKYMDK LQIKLNANRM+ GTLRG+DQFMN+VVDNTVEVNGN+K DIGMV
Sbjct: 1 MSRSGQPPDLKKYMDKKLQIKLNANRMVTGTLRGFDQFMNLVVDNTVEVNGNDKTDIGMV 60
Query: 61 VIRGNSVVTVEALEPVTRTS 80
VIRGNS+VTVEALEPV R+S
Sbjct: 61 VIRGNSIVTVEALEPVGRSS 80
>At3g11500 putative small nuclear ribonucleoprotein polypeptide G
Length = 79
Score = 143 bits (361), Expect = 1e-35
Identities = 69/79 (87%), Positives = 76/79 (95%)
Query: 1 MSRSGQPPDLKKYMDKNLQIKLNANRMIVGTLRGYDQFMNMVVDNTVEVNGNEKNDIGMV 60
MSRSGQPPDLKKYMDK LQIKLNANRM+VGTLRG+DQFMN+VVDNTVEVNG++K DIGMV
Sbjct: 1 MSRSGQPPDLKKYMDKKLQIKLNANRMVVGTLRGFDQFMNLVVDNTVEVNGDDKTDIGMV 60
Query: 61 VIRGNSVVTVEALEPVTRT 79
VIRGNS+VTVEALEPV R+
Sbjct: 61 VIRGNSIVTVEALEPVGRS 79
>At2g03870 putative snRNP splicing factor
Length = 99
Score = 58.2 bits (139), Expect = 7e-10
Identities = 27/71 (38%), Positives = 43/71 (60%), Gaps = 9/71 (12%)
Query: 9 DLKKYMDKNLQIKLNANRMIVGTLRGYDQFMNMVVDNTVEV---------NGNEKNDIGM 59
DL K++DK +Q+KL R + GTL+GYDQ +N+V+D VE ++ +G+
Sbjct: 10 DLAKFVDKGVQVKLTGGRQVTGTLKGYDQLLNLVLDEAVEFVRDHDDPLKTTDQTRRLGL 69
Query: 60 VVIRGNSVVTV 70
+V RG +V+ V
Sbjct: 70 IVCRGTAVMLV 80
>At3g14080 Sm protein, putative
Length = 128
Score = 48.5 bits (114), Expect = 6e-07
Identities = 27/69 (39%), Positives = 41/69 (59%), Gaps = 4/69 (5%)
Query: 10 LKKYMDKNLQIKLNANRMIVGTLRGYDQFMNMVVDNTVE--VNGNEKNDI--GMVVIRGN 65
L Y+D+ L + L R ++GTLR +DQF N V++ E + G + DI G+ VIRG
Sbjct: 15 LASYLDRKLLVLLRDGRKLMGTLRSFDQFANAVLEGACERVIVGEQYCDIPLGLYVIRGE 74
Query: 66 SVVTVEALE 74
+VV + L+
Sbjct: 75 NVVLIGELD 83
>At1g19120 unknown protein
Length = 128
Score = 45.1 bits (105), Expect = 6e-06
Identities = 26/69 (37%), Positives = 40/69 (57%), Gaps = 4/69 (5%)
Query: 10 LKKYMDKNLQIKLNANRMIVGTLRGYDQFMNMVVDNTVE--VNGNEKNDI--GMVVIRGN 65
L Y+DK L + L R ++G LR +DQF N V++ E + G+ DI G+ +IRG
Sbjct: 15 LAAYLDKKLLVLLRDGRKLMGLLRSFDQFANAVLEEAYERVIVGDLYCDIPLGLYIIRGE 74
Query: 66 SVVTVEALE 74
+VV + L+
Sbjct: 75 NVVLIGELD 83
>At3g59810 U6 snRNA-associated Sm-like protein
Length = 91
Score = 41.2 bits (95), Expect = 9e-05
Identities = 24/65 (36%), Positives = 36/65 (54%), Gaps = 1/65 (1%)
Query: 10 LKKYMDKNLQIKLNANRMIVGTLRGYDQFMNMVVDNTVE-VNGNEKNDIGMVVIRGNSVV 68
LK + + +KLN+ GTL D +MN+ ++ T E VNG KN G IRGN+V+
Sbjct: 19 LKSIRGRPVVVKLNSGVDYRGTLTCLDGYMNIAMEQTEEYVNGQLKNKYGDAFIRGNNVL 78
Query: 69 TVEAL 73
+ +
Sbjct: 79 YISTV 83
>At1g65700 small nuclear ribonucleoprotein, putative
Length = 98
Score = 40.8 bits (94), Expect = 1e-04
Identities = 20/70 (28%), Positives = 42/70 (59%), Gaps = 5/70 (7%)
Query: 10 LKKYMDKNLQIKLNANRMIVGTLRGYDQFMNMVVDNTVE-----VNGNEKNDIGMVVIRG 64
L+ +D+ + + N R IVG L+G+DQ N+++D + E G +++ +G+ +IRG
Sbjct: 7 LETLVDQIISVITNDGRNIVGVLKGFDQATNIILDESHERVFSTKEGVQQHVLGLYIIRG 66
Query: 65 NSVVTVEALE 74
+++ + L+
Sbjct: 67 DNIGVIGELD 76
>At2g43810 putative small nuclear ribonucleoprotein polypeptide F
Length = 91
Score = 39.3 bits (90), Expect = 4e-04
Identities = 23/62 (37%), Positives = 34/62 (54%), Gaps = 1/62 (1%)
Query: 10 LKKYMDKNLQIKLNANRMIVGTLRGYDQFMNMVVDNTVE-VNGNEKNDIGMVVIRGNSVV 68
LK K + +KLN+ G L D +MN+ ++ T E VNG KN G +RGN+V+
Sbjct: 19 LKSIRGKPVVVKLNSGVDYRGILTCLDGYMNIAMEQTEEYVNGQLKNTYGDAFVRGNNVL 78
Query: 69 TV 70
+
Sbjct: 79 YI 80
>At1g76860 unknown protein
Length = 98
Score = 35.0 bits (79), Expect = 0.007
Identities = 25/90 (27%), Positives = 46/90 (50%), Gaps = 18/90 (20%)
Query: 6 QPPDLKKY-MDKNLQIKLNANRMIVGTLRGYDQFMNMVVDN------TVEVNG------- 51
+P DL + +D+ + +KL ++R + G L +DQ +NM++ + TVE++
Sbjct: 11 EPLDLIRLSLDERIYVKLRSDRELRGKLHAFDQHLNMILGDVEETITTVEIDDETYEEIV 70
Query: 52 -NEKNDIGMVVIRGNSVVTVEALEPVTRTS 80
K I + +RG+ V+ V P RT+
Sbjct: 71 RTTKRTIEFLFVRGDGVILV---SPPLRTA 97
>At4g30220 snRNP Sm protein F - like
Length = 88
Score = 34.3 bits (77), Expect = 0.011
Identities = 20/67 (29%), Positives = 34/67 (49%), Gaps = 1/67 (1%)
Query: 8 PDLKKYMDKNLQIKLNANRMIVGTLRGYDQFMNMVVDNTVE-VNGNEKNDIGMVVIRGNS 66
P L K + +KL G L D +MN+ + NT E ++G ++G ++IR N+
Sbjct: 10 PFLNNLTGKTVIVKLKWGMEYKGFLASVDSYMNLQLGNTEEYIDGQLTGNLGEILIRCNN 69
Query: 67 VVTVEAL 73
V+ V +
Sbjct: 70 VLYVRGV 76
>At2g18740 small nuclear ribonucleoprotein E like protein
Length = 88
Score = 34.3 bits (77), Expect = 0.011
Identities = 13/42 (30%), Positives = 29/42 (68%), Gaps = 2/42 (4%)
Query: 28 IVGTLRGYDQFMNMVVDNTVEVN--GNEKNDIGMVVIRGNSV 67
I G + G+D++MN+V+D EV+ N + +G ++++G+++
Sbjct: 39 IEGRITGFDEYMNLVLDEAEEVSIKKNTRKPLGRILLKGDNI 80
>At5g44500 unknown protein
Length = 254
Score = 33.9 bits (76), Expect = 0.015
Identities = 22/91 (24%), Positives = 47/91 (51%), Gaps = 19/91 (20%)
Query: 1 MSRSGQPPDLKKYMDKNLQIKLNANRMIVGTLRGYDQFMNMVVDNTVE------VNGNEK 54
MS+S + + ++++ +++ + R ++G +D+ MN+V+ + E GN+K
Sbjct: 3 MSKSSK---MLQFINYRMRVTIQDGRQLIGKFMAFDRHMNLVLGDCEEFRKLPPAKGNKK 59
Query: 55 ND--------IGMVVIRGNSVV--TVEALEP 75
+ +G+V++RG V+ TVE P
Sbjct: 60 TNEEREERRTLGLVLLRGEEVISMTVEGPPP 90
>At4g30330 small nuclear ribonucleoprotein homolog
Length = 88
Score = 33.1 bits (74), Expect = 0.025
Identities = 13/42 (30%), Positives = 29/42 (68%), Gaps = 2/42 (4%)
Query: 28 IVGTLRGYDQFMNMVVDNTVEVNGNEK--NDIGMVVIRGNSV 67
I G + G+D++MN+V+D EV+ +K +G ++++G+++
Sbjct: 39 IEGRITGFDEYMNLVLDEAEEVSIKKKTRKPLGRILLKGDNI 80
>At5g48870 Sm-like protein (SAD1)
Length = 88
Score = 32.7 bits (73), Expect = 0.033
Identities = 12/62 (19%), Positives = 34/62 (54%), Gaps = 4/62 (6%)
Query: 10 LKKYMDKNLQIKLNANRMIVGTLRGYDQFMNMVVDNTVE----VNGNEKNDIGMVVIRGN 65
+ + + + + + ++ +VG L+G+D ++NMV+++ E G + +++ GN
Sbjct: 14 IDRCIGSKIWVIMKGDKELVGILKGFDVYVNMVLEDVTEYEITAEGRRVTKLDQILLNGN 73
Query: 66 SV 67
++
Sbjct: 74 NI 75
>At1g21190 putative protein
Length = 97
Score = 32.3 bits (72), Expect = 0.043
Identities = 23/90 (25%), Positives = 45/90 (49%), Gaps = 18/90 (20%)
Query: 6 QPPDLKKY-MDKNLQIKLNANRMIVGTLRGYDQFMNMV------VDNTVEVNG------- 51
+P DL + +++ + +KL ++R + G L +DQ +NM+ V T+E++
Sbjct: 11 EPLDLIRLSIEERIYVKLRSDRELRGKLHAFDQHLNMILGDVEEVITTIEIDDETYEEIV 70
Query: 52 -NEKNDIGMVVIRGNSVVTVEALEPVTRTS 80
K + + +RG+ V+ V P RT+
Sbjct: 71 RTTKRTVPFLFVRGDGVILV---SPPLRTT 97
>At4g20440 putative snRNP protein
Length = 257
Score = 32.0 bits (71), Expect = 0.057
Identities = 22/90 (24%), Positives = 46/90 (50%), Gaps = 18/90 (20%)
Query: 1 MSRSGQPPDLKKYMDKNLQIKLNANRMIVGTLRGYDQFMNMVVDNTVE------VNGNEK 54
MS+S + + ++++ +++ + R +VG +D+ MN+V+ + E G +
Sbjct: 3 MSKSSK---MLQFINYRMRVTIQDGRQLVGKFMAFDRHMNLVLGDCEEFRKLPPAKGKKI 59
Query: 55 ND-------IGMVVIRGNSVV--TVEALEP 75
N+ +G+V++RG V+ TVE P
Sbjct: 60 NEEREDRRTLGLVLLRGEEVISMTVEGPPP 89
>At3g07590 putative small nuclear ribonucleoprotein (Sm-D1)
Length = 114
Score = 26.9 bits (58), Expect = 1.8
Identities = 17/60 (28%), Positives = 34/60 (56%), Gaps = 3/60 (5%)
Query: 10 LKKYMDKNLQIKLNANRMIVGTLRGYDQFMNMVVDNTVEVNGNEKNDIGM--VVIRGNSV 67
L K ++ + I+L ++ GT+ G D MN + TV+++ KN + + + +RGN++
Sbjct: 7 LMKLNNETVSIELKNGTVVHGTITGVDVSMNTHL-KTVKMSLKGKNPVTLDHLSLRGNNI 65
>At1g03330 unknown protein
Length = 93
Score = 26.9 bits (58), Expect = 1.8
Identities = 11/36 (30%), Positives = 22/36 (60%)
Query: 11 KKYMDKNLQIKLNANRMIVGTLRGYDQFMNMVVDNT 46
K + + + ++L + I GTL DQ++N+ ++NT
Sbjct: 8 KDLVGQEVTVELKNDLAIRGTLHSVDQYLNIKLENT 43
>At3g56150 PROBABLE EUKARYOTIC TRANSLATION INITIATION FACTOR 3
SUBUNIT 8
Length = 900
Score = 26.2 bits (56), Expect = 3.1
Identities = 21/71 (29%), Positives = 36/71 (50%), Gaps = 6/71 (8%)
Query: 8 PDLKKYMDKNLQIKLNANRMIVGTLRGYDQFMNMVVDNTVEVNGNEKND----IGMVVIR 63
P L +M N+ K N + + L ++ N+VVD+TVE + NE + G + +
Sbjct: 296 PGLSGHMPINVWKKCVLNMLTI--LDILVKYSNIVVDDTVEPDENETSKPTDYDGKIRVW 353
Query: 64 GNSVVTVEALE 74
GN V +E ++
Sbjct: 354 GNLVAFLERVD 364
>At2g45440 putative dihydrodipicolinate synthase
Length = 365
Score = 26.2 bits (56), Expect = 3.1
Identities = 20/56 (35%), Positives = 28/56 (49%), Gaps = 6/56 (10%)
Query: 9 DLKKYMDK-NLQIKLNANRMIVGTLRGYDQFMN-----MVVDNTVEVNGNEKNDIG 58
DL+ Y D N+QI+ A +IVG G Q M+ M++ +TV G IG
Sbjct: 83 DLEAYDDLVNIQIQNGAEGVIVGGTTGEGQLMSWDEHIMLIGHTVNCFGGSIKVIG 138
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.314 0.133 0.360
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,647,374
Number of Sequences: 26719
Number of extensions: 53522
Number of successful extensions: 109
Number of sequences better than 10.0: 27
Number of HSP's better than 10.0 without gapping: 20
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 86
Number of HSP's gapped (non-prelim): 27
length of query: 80
length of database: 11,318,596
effective HSP length: 56
effective length of query: 24
effective length of database: 9,822,332
effective search space: 235735968
effective search space used: 235735968
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 52 (24.6 bits)
Lotus: description of TM0010.3.5