BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0005.12
(526 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g58470 unknown protein 42 0.001
At3g52660 putative RNA binding protein 40 0.004
At2g44710 putative heterogeneous nuclear ribonucleoprotein 40 0.004
At3g53460 RNA-binding protein cp29 protein 39 0.005
At2g37510 putative RNA-binding protein 39 0.005
At5g54580 unknown protein 39 0.007
At4g00830 unknown protein 39 0.007
At5g28390 putative protein 39 0.009
At5g40490 ribonucleoprotein -like 38 0.016
At3g16380 putative poly(A) binding protein 37 0.021
At5g28330 putative protein 36 0.060
At1g71770 polyadenylate-binding protein 5 36 0.060
At5g64200 unknown protein 35 0.078
At5g47620 RNA-binding protein-like 35 0.078
At5g19030 unknown protein 35 0.078
At3g08000 RNA-binding protein like 35 0.078
At1g26150 Pto kinase interactor, putative 35 0.078
At1g22760 putative polyA-binding protein, PAB3 35 0.078
At4g16290 FCA delta protein 35 0.10
At4g16280 FCA gamma protein 35 0.10
>At1g58470 unknown protein
Length = 360
Score = 41.6 bits (96), Expect = 0.001
Identities = 23/84 (27%), Positives = 45/84 (53%), Gaps = 2/84 (2%)
Query: 65 FPLFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDVMKGRS 124
+ LFV GIA + ++ F ++G + + +++ G+ FGFVR+AN DV+K +
Sbjct: 6 YKLFVGGIAKETSEEALKQYFSRYGAVLEAVVAKEKVTGKPRGFGFVRFANDCDVVK--A 63
Query: 125 RKQSYVSMAPPTTVKDSRAKERLH 148
+ ++ + P V+ + K L+
Sbjct: 64 LRDTHFILGKPVDVRKAIRKHELY 87
>At3g52660 putative RNA binding protein
Length = 471
Score = 39.7 bits (91), Expect = 0.004
Identities = 22/82 (26%), Positives = 37/82 (44%), Gaps = 8/82 (9%)
Query: 48 GGDARVARVVAPPARTCFPLFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFR 107
GGD+ ++V A L++ + + ++ LF HGK+ V + + R
Sbjct: 259 GGDSSASQVKA--------LYIKNLPRDITQERLKALFEHHGKILKVVIPPAKPGKEDSR 310
Query: 108 FGFVRYANREDVMKGRSRKQSY 129
+GFV YA R VM+ + Y
Sbjct: 311 YGFVHYAERTSVMRALKNTERY 332
>At2g44710 putative heterogeneous nuclear ribonucleoprotein
Length = 300
Score = 39.7 bits (91), Expect = 0.004
Identities = 21/51 (41%), Positives = 27/51 (52%)
Query: 67 LFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANRE 117
+F+DG+ S N VRDL +GKL V L R R+ FGFV + E
Sbjct: 226 IFIDGLLPSWNEERVRDLLKPYGKLEKVELARNMPSARRKDFGFVTFDTHE 276
>At3g53460 RNA-binding protein cp29 protein
Length = 342
Score = 39.3 bits (90), Expect = 0.005
Identities = 28/108 (25%), Positives = 46/108 (41%), Gaps = 14/108 (12%)
Query: 14 SPKSGGITSRQAAGGTFGDLSSVDDEGDDEGEQDGGDARVARVVAPPARTCFPLFVDGIA 73
S + GG S+++ GG G S G G G R L+V ++
Sbjct: 220 SERGGGYGSQRSGGGYGGSQRSSYGSGSGSGSGSGSGNR--------------LYVGNLS 265
Query: 74 DSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDVMK 121
V+ + +LF + GK+ + R+ GR FGFV ++ ++V K
Sbjct: 266 WGVDDMALENLFNEQGKVVEARVIYDRDSGRSKGFGFVTLSSSQEVQK 313
Score = 28.9 bits (63), Expect = 7.3
Identities = 35/135 (25%), Positives = 54/135 (39%), Gaps = 19/135 (14%)
Query: 33 LSSVDDEGDDEGEQDGGDARVARVVAPPARTCFP----LFVDGIADSVNYFHVRDLFLQH 88
+SS + +D+ DG D+ AP R F LFV ++ +V+ + LF
Sbjct: 69 VSSDFEVEEDDMFADGDDS------APVERNSFSPDLKLFVGNLSFNVDSAQLAQLFESA 122
Query: 89 GKLTNVFLQRKRNRGRKFRFGFVRYANREDVMKGRSRKQSY--------VSMAPPTTVKD 140
G + V + + GR FGFV + +V + Y V+ PP K
Sbjct: 123 GNVEMVEVIYDKVTGRSRGFGFVTMSTAAEVEAAAQQFNGYEFEGRPLRVNAGPPPP-KR 181
Query: 141 SRAKERLHLSGGFAS 155
+ R SGG+ S
Sbjct: 182 EESFSRGPRSGGYGS 196
>At2g37510 putative RNA-binding protein
Length = 142
Score = 39.3 bits (90), Expect = 0.005
Identities = 30/106 (28%), Positives = 46/106 (43%), Gaps = 11/106 (10%)
Query: 67 LFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDVMKGRSRK 126
LFV G++ ++D F G+L + + R+ GR FGFV YA ED K ++
Sbjct: 36 LFVSGLSRLTTNEKLQDAFASFGQLVDARVITDRDSGRSKGFGFVTYATIEDAEKAKAEM 95
Query: 127 QS--------YVSMAPPTTVKDSRAKERLHLS--GGFASSSR-SWC 161
+ +V A P + +E L S GF ++ WC
Sbjct: 96 NAKFLDGWVIFVDPARPREPRRPLQQEPLRPSSESGFTTNKTIGWC 141
>At5g54580 unknown protein
Length = 156
Score = 38.9 bits (89), Expect = 0.007
Identities = 23/64 (35%), Positives = 30/64 (45%)
Query: 59 PPARTCFPLFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANRED 118
P A LFV G++ +R F Q G++ + + R G FGFVRYA ED
Sbjct: 50 PQAEPSTNLFVSGLSKRTTSEGLRTAFAQFGEVADAKVVTDRVSGYSKGFGFVRYATLED 109
Query: 119 VMKG 122
KG
Sbjct: 110 SAKG 113
>At4g00830 unknown protein
Length = 495
Score = 38.9 bits (89), Expect = 0.007
Identities = 25/83 (30%), Positives = 40/83 (48%), Gaps = 3/83 (3%)
Query: 67 LFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDVMKGRSRK 126
L+V I ++ + +++LF +HG++T + +G K FGFV YA R +K
Sbjct: 294 LYVKNIPENTSTEQLKELFQRHGEVTKIV--TPPGKGGKRDFGFVHYAERSSALKAVKDT 351
Query: 127 QSY-VSMAPPTTVKDSRAKERLH 148
+ Y V+ P V ER H
Sbjct: 352 ERYEVNGQPLEVVLAKPQAERKH 374
>At5g28390 putative protein
Length = 380
Score = 38.5 bits (88), Expect = 0.009
Identities = 27/116 (23%), Positives = 48/116 (41%), Gaps = 15/116 (12%)
Query: 49 GDARVARVVAPPARTCFPLFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRF 108
GD+ ++V A L++ + + ++ LF HGK+ V + + R+
Sbjct: 26 GDSSASQVKA--------LYIKNLPRDITQERLKALFEHHGKILKVVIPPAKPGKEDSRY 77
Query: 109 GFVRYANREDVMKGRSRKQSYVSMAPPTTVKDSRAKERLHLSGGFASSSRSWCDVL 164
GFV YA R VM+ + Y + S + L +GG A+ S ++
Sbjct: 78 GFVHYAERTSVMRALKNTERY-------EIDASAYSQPLMHAGGHAAGGMSMMPIM 126
>At5g40490 ribonucleoprotein -like
Length = 423
Score = 37.7 bits (86), Expect = 0.016
Identities = 27/96 (28%), Positives = 42/96 (43%), Gaps = 5/96 (5%)
Query: 29 TFGDLSSVDDEGDDEGEQDGGDARVARVVAPPARTCFP-----LFVDGIADSVNYFHVRD 83
T+ D S VD D G + R + + + +FV GI SV+ ++
Sbjct: 89 TYADSSVVDKVIQDNHIIIGKQVEIKRTIPRGSMSSNDFKTKKIFVGGIPSSVDDDEFKE 148
Query: 84 LFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDV 119
F+Q G+L + R + GR FGFV Y + + V
Sbjct: 149 FFMQFGELKEHQIMRDHSTGRSRGFGFVTYESEDMV 184
Score = 32.0 bits (71), Expect = 0.86
Identities = 22/86 (25%), Positives = 36/86 (41%), Gaps = 10/86 (11%)
Query: 36 VDDEGDDEGEQDGGDARVARVVAPPARTCFPLFVDGIADSVNYFHVRDLFLQHGKLTNVF 95
++D+ D GG A + FV G+A F ++G++T+
Sbjct: 23 IEDDDDKSQPHSGGGVDSAGKI----------FVGGLARETTSAEFLKHFGKYGEITDSV 72
Query: 96 LQRKRNRGRKFRFGFVRYANREDVMK 121
+ + R G+ FGFV YA+ V K
Sbjct: 73 IMKDRKTGQPRGFGFVTYADSSVVDK 98
>At3g16380 putative poly(A) binding protein
Length = 537
Score = 37.4 bits (85), Expect = 0.021
Identities = 22/89 (24%), Positives = 45/89 (49%), Gaps = 1/89 (1%)
Query: 57 VAPPARTCFPLFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANR 116
+A P L+V +++S+N +R++F +G++ + + N GR FGFV ++N
Sbjct: 296 IAKPNMRWSNLYVKNLSESMNETRLREIFGCYGQIVSAKVMCHEN-GRSKGFGFVCFSNC 354
Query: 117 EDVMKGRSRKQSYVSMAPPTTVKDSRAKE 145
E+ + + ++ P V+ + KE
Sbjct: 355 EESKQAKRYLNGFLVDGKPIVVRVAERKE 383
Score = 31.2 bits (69), Expect = 1.5
Identities = 17/55 (30%), Positives = 30/55 (53%), Gaps = 1/55 (1%)
Query: 67 LFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDVMK 121
++V + ++V + LF Q+G +++V + R GR FGFV + N E+ K
Sbjct: 204 VYVKNLIETVTDDCLHTLFSQYGTVSSVVVMRD-GMGRSRGFGFVNFCNPENAKK 257
>At5g28330 putative protein
Length = 180
Score = 35.8 bits (81), Expect = 0.060
Identities = 22/98 (22%), Positives = 40/98 (40%), Gaps = 7/98 (7%)
Query: 67 LFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDVMKGRSRK 126
L++ + + ++ L HGK+ V + + R+GFV YA R VM+
Sbjct: 36 LYIKNLPRDITQERLKALLEHHGKILKVVIPPAKPGKEDSRYGFVHYAERTSVMRALKNT 95
Query: 127 QSYVSMAPPTTVKDSRAKERLHLSGGFASSSRSWCDVL 164
+ Y + S + L +GG A+ S ++
Sbjct: 96 ERY-------EIDASAYSQPLMHAGGHAAGGMSMMPIM 126
>At1g71770 polyadenylate-binding protein 5
Length = 668
Score = 35.8 bits (81), Expect = 0.060
Identities = 23/75 (30%), Positives = 42/75 (55%), Gaps = 9/75 (12%)
Query: 67 LFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDV------M 120
L++ + DSVN ++++F ++G +T+ + ++G FGFV Y+N E+ M
Sbjct: 330 LYLKNLDDSVNDEKLKEMFSEYGNVTSCKVMMN-SQGLSRGFGFVAYSNPEEALLAMKEM 388
Query: 121 KGR--SRKQSYVSMA 133
G+ RK YV++A
Sbjct: 389 NGKMIGRKPLYVALA 403
Score = 31.2 bits (69), Expect = 1.5
Identities = 27/89 (30%), Positives = 39/89 (43%), Gaps = 9/89 (10%)
Query: 67 LFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDVMKGRSRK 126
L+V + SVN H+ DLF Q + N+ + R R + +V +AN ED SR
Sbjct: 47 LYVGDLDPSVNESHLLDLFNQVAPVHNLRVCRDLTH-RSLGYAYVNFANPEDA----SRA 101
Query: 127 QSYVSMAP----PTTVKDSRAKERLHLSG 151
++ AP P + S LSG
Sbjct: 102 MESLNYAPIRDRPIRIMLSNRDPSTRLSG 130
>At5g64200 unknown protein
Length = 303
Score = 35.4 bits (80), Expect = 0.078
Identities = 19/56 (33%), Positives = 28/56 (49%)
Query: 84 LFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDVMKGRSRKQSYVSMAPPTTVK 139
LF ++GK+ +VF+ R R G F FVRY +++ K R V TV+
Sbjct: 35 LFAKYGKVVDVFIPRDRRTGDSRGFAFVRYKYKDEAHKAVERLDGRVVDGREITVQ 90
>At5g47620 RNA-binding protein-like
Length = 431
Score = 35.4 bits (80), Expect = 0.078
Identities = 19/55 (34%), Positives = 27/55 (48%)
Query: 67 LFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDVMK 121
+FV G+A SV + F Q G +T+V + R FGF+ Y + E V K
Sbjct: 108 IFVGGLASSVTEAEFKKYFAQFGMITDVVVMYDHRTQRPRGFGFISYDSEEAVDK 162
Score = 32.0 bits (71), Expect = 0.86
Identities = 16/49 (32%), Positives = 26/49 (52%)
Query: 67 LFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYAN 115
LF+ GI+ + +RD F G++ + + R GR FGFV +A+
Sbjct: 8 LFIGGISWETSEDRLRDYFHSFGEVLEAVIMKDRATGRARGFGFVVFAD 56
>At5g19030 unknown protein
Length = 172
Score = 35.4 bits (80), Expect = 0.078
Identities = 16/52 (30%), Positives = 31/52 (58%)
Query: 67 LFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANRED 118
LFV G +DSV+ ++ +F + G++TNV + + +G+V + ++ED
Sbjct: 79 LFVKGFSDSVSEGRLKKVFSEFGQVTNVKIIANERTRQSLGYGYVWFNSKED 130
>At3g08000 RNA-binding protein like
Length = 143
Score = 35.4 bits (80), Expect = 0.078
Identities = 17/57 (29%), Positives = 30/57 (51%)
Query: 67 LFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDVMKGR 123
LF+ G++ SV+ ++D F G++ V + + GR FGFV +A D + +
Sbjct: 43 LFIGGLSWSVDEQSLKDAFSSFGEVAEVRIAYDKGSGRSRGFGFVDFAEEGDALSAK 99
>At1g26150 Pto kinase interactor, putative
Length = 760
Score = 35.4 bits (80), Expect = 0.078
Identities = 22/61 (36%), Positives = 27/61 (44%), Gaps = 4/61 (6%)
Query: 433 PVLDFSPPRPRPRGRPKRKKEVKRGLEGSRRPQGHPRKSGTMDLDAAPSPSHPSPPPQLH 492
P D P P P G PKR+++ GS+RP P PSP PSPP +
Sbjct: 210 PPSDSEHPSPPPPGHPKRREQPPP--PGSKRPTPSPPSPSDSKRPVHPSP--PSPPEETL 265
Query: 493 P 493
P
Sbjct: 266 P 266
>At1g22760 putative polyA-binding protein, PAB3
Length = 660
Score = 35.4 bits (80), Expect = 0.078
Identities = 23/90 (25%), Positives = 46/90 (50%), Gaps = 3/90 (3%)
Query: 67 LFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDVMKGRSRK 126
L++ + DSV+ ++++F ++G +T+ + +G FGFV Y+N E+ ++ S
Sbjct: 334 LYLKNLDDSVDDEKLKEMFSEYGNVTSSKVMLNP-QGMSRGFGFVAYSNPEEALRALSEM 392
Query: 127 QSYVSMAPPTTVKDSRAKE--RLHLSGGFA 154
+ P + ++ KE R HL F+
Sbjct: 393 NGKMIGRKPLYIALAQRKEDRRAHLQALFS 422
>At4g16290 FCA delta protein
Length = 533
Score = 35.0 bits (79), Expect = 0.10
Identities = 19/56 (33%), Positives = 30/56 (52%), Gaps = 1/56 (1%)
Query: 65 FPLFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDVM 120
F LFV + V ++FLQ G + +V+L R R + GFV+Y+++E M
Sbjct: 211 FKLFVGSLNKQATEKEVEEIFLQFGHVEDVYLMRDEYRQSR-GCGFVKYSSKETAM 265
Score = 31.2 bits (69), Expect = 1.5
Identities = 16/52 (30%), Positives = 26/52 (49%)
Query: 67 LFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANRED 118
LFV + + +R F QHG + V L + + G++ FV+YA +D
Sbjct: 122 LFVGSVPRTATEEEIRPYFEQHGNVLEVALIKDKRTGQQQGCCFVKYATSKD 173
>At4g16280 FCA gamma protein
Length = 747
Score = 35.0 bits (79), Expect = 0.10
Identities = 19/56 (33%), Positives = 30/56 (52%), Gaps = 1/56 (1%)
Query: 65 FPLFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANREDVM 120
F LFV + V ++FLQ G + +V+L R R + GFV+Y+++E M
Sbjct: 211 FKLFVGSLNKQATEKEVEEIFLQFGHVEDVYLMRDEYRQSR-GCGFVKYSSKETAM 265
Score = 31.2 bits (69), Expect = 1.5
Identities = 16/52 (30%), Positives = 26/52 (49%)
Query: 67 LFVDGIADSVNYFHVRDLFLQHGKLTNVFLQRKRNRGRKFRFGFVRYANRED 118
LFV + + +R F QHG + V L + + G++ FV+YA +D
Sbjct: 122 LFVGSVPRTATEEEIRPYFEQHGNVLEVALIKDKRTGQQQGCCFVKYATSKD 173
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.320 0.138 0.424
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,830,103
Number of Sequences: 26719
Number of extensions: 597774
Number of successful extensions: 2552
Number of sequences better than 10.0: 81
Number of HSP's better than 10.0 without gapping: 48
Number of HSP's successfully gapped in prelim test: 34
Number of HSP's that attempted gapping in prelim test: 2404
Number of HSP's gapped (non-prelim): 168
length of query: 526
length of database: 11,318,596
effective HSP length: 104
effective length of query: 422
effective length of database: 8,539,820
effective search space: 3603804040
effective search space used: 3603804040
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0005.12