BLAST2 result
BLASTP 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0004a.6
(418 letters)
Database: ara_mips
26,719 sequences; 11,318,596 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g38670 putative phospholipid cytidylyltransferase 529 e-150
At4g15130 CTP:phosphorylcholine cytidylyltransferase (AtCCT2) 116 2e-26
At2g32260 putative phospholipid cytidylyltransferase 114 8e-26
At5g23330 unknown protein 37 0.020
At5g08345 unknown protein 32 0.85
At1g67630 DNA polymerase alpha subunit like protein 31 1.1
At4g39450 hypothetical protein 31 1.5
At5g16780 putative protein 30 1.9
At3g12010 unknown protein 30 2.5
At5g66410 unknown protein 29 4.2
At2g45900 hypothetical protein 29 4.2
At4g28420 tyrosine transaminase-like protein 29 5.5
At2g18250 hypothetical protein 29 5.5
At5g14990 putative protein 28 7.2
At5g03300 adenosine kinase (MOK16.21) 28 7.2
At3g51520 unknown protein 28 9.4
At3g21660 ubiquitin-regulatory protein, putative 28 9.4
>At2g38670 putative phospholipid cytidylyltransferase
Length = 421
Score = 529 bits (1362), Expect = e-150
Identities = 263/393 (66%), Positives = 321/393 (80%), Gaps = 21/393 (5%)
Query: 39 GIGHLPLPFPWSNSGIFHKKKSGKRRVRVYMDGCFDLMHYGHANALRQAKALGDELVVGL 98
G+G L L PW I K+K VRVYMDGCFD+MHYGH NALRQA+ALGD+LVVG+
Sbjct: 37 GLG-LGLSCPWR---ILRKRKP----VRVYMDGCFDMMHYGHCNALRQARALGDQLVVGV 88
Query: 99 VNDEEILATKGPPVLTMAERLALVSGLKWVDEVITDAPYAITEQFLHRLFHEYKIDYVIH 158
V+DEEI+A KGPPV + ER+ +V +KWVDEVI+DAPYAITE F+ +LF EY+IDY+IH
Sbjct: 89 VSDEEIIANKGPPVTPLHERMTMVKAVKWVDEVISDAPYAITEDFMKKLFDEYQIDYIIH 148
Query: 159 GDDPCLLPDGTDAYAAAKKAGRYKQIKRTEGVSSTDIVGRIMSSLKDQKTCEDQNGTDVK 218
GDDPC+LPDGTDAYA AKKAGRYKQIKRTEGVSSTDIVGR++ ++++ + + + ++
Sbjct: 149 GDDPCVLPDGTDAYALAKKAGRYKQIKRTEGVSSTDIVGRMLLCVRERSISDTHSRSSLQ 208
Query: 219 RQ-----------EESMSKDSHISQFLPTSRRIVQFSNGKGPGPNARIVYIDGAFDLFHA 267
RQ + + S + +S FLPTSRRIVQFSNGKGPGP+ARI+YIDGAFDLFHA
Sbjct: 209 RQFSHGHSSPKFEDGASSAGTRVSHFLPTSRRIVQFSNGKGPGPDARIIYIDGAFDLFHA 268
Query: 268 GHVEILKRARELGDFLLVGIHSDETVSEHRGNHYPIMHLHERSLSVLACRHVDEVIIGAP 327
GHVEIL+RARELGDFLLVGIH+D+TVS RG H PIM+LHERSLSVLACR+VDEVIIGAP
Sbjct: 269 GHVEILRRARELGDFLLVGIHNDQTVSAKRGAHRPIMNLHERSLSVLACRYVDEVIIGAP 328
Query: 328 WEITKDMITTFNISLVVHGTVAEKS--LHSERDPYEVPKSMGMFRLLESPKDITTTTVAQ 385
WE+++D ITTF+ISLVVHGTVAE E +PY VP SMG+F++L+SP DITT+T+ +
Sbjct: 329 WEVSRDTITTFDISLVVHGTVAESDDFRKEEDNPYSVPISMGIFQVLDSPLDITTSTIIR 388
Query: 386 RILANHEAYEKRNAKKSQSEKRYYEEKNYVSGD 418
RI+ANHEAY+KRNAKK SEK+YYE+K++VSGD
Sbjct: 389 RIVANHEAYQKRNAKKEASEKKYYEQKSFVSGD 421
>At4g15130 CTP:phosphorylcholine cytidylyltransferase (AtCCT2)
Length = 300
Score = 116 bits (291), Expect = 2e-26
Identities = 67/145 (46%), Positives = 86/145 (59%), Gaps = 8/145 (5%)
Query: 60 SGKRRVRVYMDGCFDLMHYGHANALRQAKAL--GDELVVGLVNDEEILATKGPPVLTMAE 117
S R VRVY DG FDL H+GHA A+ QAK L+VG NDE KG V+T +E
Sbjct: 16 SSDRPVRVYADGIFDLFHFGHARAIEQAKKSFPNTYLLVGCCNDEITNKFKGKTVMTESE 75
Query: 118 RLALVSGLKWVDEVITDAPYAITEQFLHRLFHEYKIDYVIHGDDPCLLPD--GTDAYAAA 175
R + KWVDEVI DAP+ +T +FL + +KIDYV H P G D Y
Sbjct: 76 RYESLRHCKWVDEVIPDAPWVLTTEFLDK----HKIDYVAHDALPYADTSGAGNDVYEFV 131
Query: 176 KKAGRYKQIKRTEGVSSTDIVGRIM 200
K G++K+ KRTEG+S++DI+ RI+
Sbjct: 132 KSIGKFKETKRTEGISTSDIIMRIV 156
Score = 84.3 bits (207), Expect = 1e-16
Identities = 50/146 (34%), Positives = 79/146 (53%), Gaps = 5/146 (3%)
Query: 256 VYIDGAFDLFHAGHVEILKRAREL--GDFLLVGIHSDETVSEHRGNHYPIMHLHERSLSV 313
VY DG FDLFH GH +++A++ +LLVG +DE ++ +G +M ER S+
Sbjct: 23 VYADGIFDLFHFGHARAIEQAKKSFPNTYLLVGCCNDEITNKFKGK--TVMTESERYESL 80
Query: 314 LACRHVDEVIIGAPWEITKDMITTFNISLVVHGTVA-EKSLHSERDPYEVPKSMGMFRLL 372
C+ VDEVI APW +T + + I V H + + + D YE KS+G F+
Sbjct: 81 RHCKWVDEVIPDAPWVLTTEFLDKHKIDYVAHDALPYADTSGAGNDVYEFVKSIGKFKET 140
Query: 373 ESPKDITTTTVAQRILANHEAYEKRN 398
+ + I+T+ + RI+ ++ Y RN
Sbjct: 141 KRTEGISTSDIIMRIVKDYNQYVLRN 166
>At2g32260 putative phospholipid cytidylyltransferase
Length = 332
Score = 114 bits (286), Expect = 8e-26
Identities = 64/143 (44%), Positives = 85/143 (58%), Gaps = 9/143 (6%)
Query: 63 RRVRVYMDGCFDLMHYGHANALRQAKAL---GDELVVGLVNDEEILATKGPPVLTMAERL 119
R VRVY DG +DL H+GHA +L QAK L+VG NDE KG V+T ER
Sbjct: 33 RPVRVYADGIYDLFHFGHARSLEQAKLAFPNNTYLLVGCCNDETTHKYKGRTVMTAEERY 92
Query: 120 ALVSGLKWVDEVITDAPYAITEQFLHRLFHEYKIDYVIHGDDPCLLPDGT--DAYAAAKK 177
+ KWVDEVI DAP+ + ++FL + ++IDYV H P G D Y KK
Sbjct: 93 ESLRHCKWVDEVIPDAPWVVNQEFLDK----HQIDYVAHDSLPYADSSGAGKDVYEFVKK 148
Query: 178 AGRYKQIKRTEGVSSTDIVGRIM 200
GR+K+ +RTEG+S++DI+ RI+
Sbjct: 149 VGRFKETQRTEGISTSDIIMRIV 171
Score = 88.2 bits (217), Expect = 8e-18
Identities = 51/155 (32%), Positives = 83/155 (52%), Gaps = 6/155 (3%)
Query: 256 VYIDGAFDLFHAGHVEILKRAREL---GDFLLVGIHSDETVSEHRGNHYPIMHLHERSLS 312
VY DG +DLFH GH L++A+ +LLVG +DET +++G +M ER S
Sbjct: 37 VYADGIYDLFHFGHARSLEQAKLAFPNNTYLLVGCCNDETTHKYKGR--TVMTAEERYES 94
Query: 313 VLACRHVDEVIIGAPWEITKDMITTFNISLVVHGTVA-EKSLHSERDPYEVPKSMGMFRL 371
+ C+ VDEVI APW + ++ + I V H ++ S + +D YE K +G F+
Sbjct: 95 LRHCKWVDEVIPDAPWVVNQEFLDKHQIDYVAHDSLPYADSSGAGKDVYEFVKKVGRFKE 154
Query: 372 LESPKDITTTTVAQRILANHEAYEKRNAKKSQSEK 406
+ + I+T+ + RI+ ++ Y RN + S +
Sbjct: 155 TQRTEGISTSDIIMRIVKDYNQYVMRNLDRGYSRE 189
>At5g23330 unknown protein
Length = 354
Score = 37.0 bits (84), Expect = 0.020
Identities = 36/171 (21%), Positives = 71/171 (41%), Gaps = 21/171 (12%)
Query: 71 GCFDLMHYGHANALRQAKALGDELVVGLVNDEEILATK-GPPVLTMAERLALVSGLKWVD 129
G FD +H GH QA +G ++ V E+L K P++ +R ++S W
Sbjct: 90 GKFDALHIGHRELAIQAARIGTPYLLSFVGLAEVLGWKPRAPIVAKCDRKRVLS--SWAS 147
Query: 130 EVITDAP-----------YAITEQFLHRLFHEYKIDYVIHGDDPCLLPDGTDAYAAAKKA 178
AP + +QF+ +L E ++ V+ G++ Y A+ A
Sbjct: 148 YCGNIAPVEFEIEFASVRHLNPQQFVEKLSRELRVCGVVAGENYRF------GYRASGDA 201
Query: 179 GRYKQIKRTEGVSSTDIVGRIMSSLKDQKTCEDQNGTDVKRQEESMSKDSH 229
++ + G+S+ I+ +M + E+++ +R + S ++ H
Sbjct: 202 SELVRLCKDFGISAY-IINSVMDKNQVSVNTEEEDSKSKERGQVSSTRVRH 251
>At5g08345 unknown protein
Length = 367
Score = 31.6 bits (70), Expect = 0.85
Identities = 36/190 (18%), Positives = 78/190 (40%), Gaps = 28/190 (14%)
Query: 71 GCFDLMHYGHANALRQAKALGDELVVGLVNDEEILATK-GPPVLTMAERLALVSGLKWVD 129
G FD +H GH QA +G ++ V E+L + P++ +R +++ W
Sbjct: 106 GKFDALHIGHRELTIQASRIGAPYLLSFVGMAEVLGWEPRAPIVAKCDRQRVLT--SWAS 163
Query: 130 EVITDAP-----------YAITEQFLHRLFHEYKIDYVIHGDDPCLLPDGTDAYAAAKKA 178
AP + QF+ +L E ++ V+ G++ Y A+ A
Sbjct: 164 YCGDRAPEEYEIEFASVRHLTPRQFVEKLSKELRVCGVVAGENYRF------GYKASGDA 217
Query: 179 GRYKQIKRTEGVSSTDIVGRIM-----SSLKDQKTCEDQNGTDVKRQEESMSKDS--HIS 231
++ G+++ I+ +M S+ +D +D+ R ++++ ++S
Sbjct: 218 SELVRLCEECGITAC-IINSVMDMKQGSAKRDSGDSKDRGQVSSTRVRQALAAGDMRYVS 276
Query: 232 QFLPTSRRIV 241
+ L + R++
Sbjct: 277 ELLGRAHRLI 286
>At1g67630 DNA polymerase alpha subunit like protein
Length = 620
Score = 31.2 bits (69), Expect = 1.1
Identities = 22/67 (32%), Positives = 31/67 (45%), Gaps = 7/67 (10%)
Query: 210 EDQNGTDVKRQEESMSKDSHISQFLPTSRRIVQFSNGKGPGPNARIVYIDGAFDLFHAGH 269
E+ N DV+ E+ + K + TS+R NG GP P R +Y D D F A
Sbjct: 170 ENGNNNDVENSEDDIIKR------VQTSQRCSLKVNGSGPEPGCRFMY-DRTEDRFSALE 222
Query: 270 VEILKRA 276
I++ A
Sbjct: 223 YRIVRHA 229
>At4g39450 hypothetical protein
Length = 1553
Score = 30.8 bits (68), Expect = 1.5
Identities = 28/89 (31%), Positives = 42/89 (46%), Gaps = 5/89 (5%)
Query: 260 GAFDLFHAGHVEILKRARELGDFLLVGIHSDETVSEHRGNHYPIMHLHERS-----LSVL 314
G F L HA VE ARE+ + LL+ + ++ YP+ L E L+V
Sbjct: 762 GLFFLRHAEVVEKDLPAREIYELLLLSLQWLSGLTTLSHPVYPLHLLREIETRVWLLAVE 821
Query: 315 ACRHVDEVIIGAPWEITKDMITTFNISLV 343
A HV V +P I KDM+ ++ +L+
Sbjct: 822 AESHVKNVGAFSPSSIGKDMVNGYSSNLI 850
>At5g16780 putative protein
Length = 820
Score = 30.4 bits (67), Expect = 1.9
Identities = 19/61 (31%), Positives = 30/61 (49%), Gaps = 1/61 (1%)
Query: 164 LLPDGTDAYAAAKKAGRYKQIKRTEGVSSTDIVGRIMSSLKDQKTCEDQNGTDVKRQEES 223
L D D Y + +KA R IK+ E S V +++S +Q T +D T + QE +
Sbjct: 485 LADDAEDLYKSLEKARRLALIKKEEAGSGPQAVAHLVASSTNQ-TTDDNTTTGDETQENT 543
Query: 224 M 224
+
Sbjct: 544 V 544
>At3g12010 unknown protein
Length = 685
Score = 30.0 bits (66), Expect = 2.5
Identities = 21/76 (27%), Positives = 38/76 (49%), Gaps = 6/76 (7%)
Query: 171 AYAAAKKAGRYKQIKRTEGVSSTDIVGRIMSSLKDQKTCEDQNGTDVKRQ-EESMSKDSH 229
AY+ + KAG YK+IK +ST G S + + Q+G+++ + E ++S S
Sbjct: 424 AYSYSVKAGTYKEIKNENATASTPTPGASPDSERHR-----QSGSNIDEEVEMNLSSGSQ 478
Query: 230 ISQFLPTSRRIVQFSN 245
+ F ++ Q S+
Sbjct: 479 ENMFCADEQQESQLSS 494
>At5g66410 unknown protein
Length = 230
Score = 29.3 bits (64), Expect = 4.2
Identities = 33/137 (24%), Positives = 53/137 (38%), Gaps = 20/137 (14%)
Query: 280 GDFLLVGIHSDETVSEHRGNHYPIMHLHERSLSVLACRHVDEVII-----GAPWEITKDM 334
GDFL S++ + + + ++ L LA RHVD I AP+ +TK
Sbjct: 92 GDFLGEVTRSEKVICHFYHKEFYRCKIMDKHLKTLAPRHVDTKFIKMDAENAPFFVTKLA 151
Query: 335 ITTFNISLVVHGTVAEKSLHSERDPYEVPKSMGMFRLLESPKDITTTTVAQRILANHEAY 394
I T ++ +A L +D L + D +TT + ++
Sbjct: 152 IKTLPCVILFSKGIAMDRLVGFQD-------------LGAKDDFSTTKLENLLVKKGMLS 198
Query: 395 EKRNAKKSQSEKRYYEE 411
EKR K+ E Y+E
Sbjct: 199 EKR--KEEDEEDYEYQE 213
>At2g45900 hypothetical protein
Length = 720
Score = 29.3 bits (64), Expect = 4.2
Identities = 19/64 (29%), Positives = 34/64 (52%), Gaps = 3/64 (4%)
Query: 348 VAEKSLHSERDPYEVPKSMGMFRLLESPKDITTTTVAQRILA-NHEAYEKRNAKKSQSEK 406
+AE + ++ EVP+ +G ++L P+ T + LA +H+ EK N ++ S+
Sbjct: 342 LAEGDIDADLPDKEVPRILG--KILALPEFFTPENSPRVTLALDHQIIEKPNIQQCSSKD 399
Query: 407 RYYE 410
YYE
Sbjct: 400 YYYE 403
>At4g28420 tyrosine transaminase-like protein
Length = 389
Score = 28.9 bits (63), Expect = 5.5
Identities = 19/55 (34%), Positives = 28/55 (50%), Gaps = 8/55 (14%)
Query: 358 DPYEVPKSMGMFRLLESPKDIT--TTTVAQRIL------ANHEAYEKRNAKKSQS 404
DP + KS GM + ++ DIT TT+ Q L AN E + K+N+ Q+
Sbjct: 286 DPRGILKSTGMVQSIQQNLDITPDATTIVQAALPEILGKANKELFAKKNSMLKQN 340
>At2g18250 hypothetical protein
Length = 176
Score = 28.9 bits (63), Expect = 5.5
Identities = 16/40 (40%), Positives = 21/40 (52%), Gaps = 1/40 (2%)
Query: 249 PGPNARIVYIDGAFDLFHAGHVEILKRARELG-DFLLVGI 287
P + V + G FD H GH LK A EL D ++VG+
Sbjct: 11 PANSFGAVVLGGTFDRLHDGHRMFLKAAAELARDRIVVGV 50
>At5g14990 putative protein
Length = 666
Score = 28.5 bits (62), Expect = 7.2
Identities = 51/232 (21%), Positives = 90/232 (37%), Gaps = 27/232 (11%)
Query: 182 KQIKRTEGV---SSTDIVGRIMSSLKDQKTCEDQNGTDVKRQEESMSK--DSHISQFLPT 236
K IK E + S ++ + S+K QK+C NG+ KR + + DS +S
Sbjct: 332 KLIKSHESIIRRKSEELAPPKIESIKRQKSC---NGSSSKRAIDDIVSGLDSLMSLNTKL 388
Query: 237 SRRIVQFSNGKGPGPNARIVYIDGAFDLFHAGHVEILKRARELGDFLLVGIHSDETVSEH 296
+ +G + +V D D++ +K + F SD + E
Sbjct: 389 FEHLFDDDDGDRHEHHPEVVMDDNLDDVW-------MKMQKNNSVF------SDNAIEEK 435
Query: 297 RGNHYPIMHLHERSLSVLACRHVDEVIIGAPWEITKDMITTFNISLVVHGTVAEKSLHSE 356
+M L + L++L DE+ E ++ I + I V ++L+ E
Sbjct: 436 EDTEIRLMILEDTYLTLLKGLKADEITNNRKAEEEEEEIKSEKIESEVKCMDCLENLNRE 495
Query: 357 RDPYEVPKSMGMFRLLESPKDITTTTVAQRI---LANHEAYEKRNAKKSQSE 405
+D YE+ FR + I T + + + + NHE E N + + E
Sbjct: 496 KD-YEILLEDEEFR--QELSWIIVTELLREVSETVENHEKIEANNKRVIEEE 544
>At5g03300 adenosine kinase (MOK16.21)
Length = 345
Score = 28.5 bits (62), Expect = 7.2
Identities = 14/54 (25%), Positives = 26/54 (47%)
Query: 249 PGPNARIVYIDGAFDLFHAGHVEILKRARELGDFLLVGIHSDETVSEHRGNHYP 302
P P ++V +GA D F G + L + + + + + G ++ V + G YP
Sbjct: 286 PLPKEKLVDTNGAGDAFVGGFMSQLVKEKSIEECVKAGCYASNVVIQRSGCTYP 339
>At3g51520 unknown protein
Length = 314
Score = 28.1 bits (61), Expect = 9.4
Identities = 19/54 (35%), Positives = 25/54 (46%), Gaps = 4/54 (7%)
Query: 312 SVLACRHVDEVIIGAPWEITKDMITTFNISLVVHGTVAEKSLHSERDPYEVPKS 365
S L CR V++G P E+TK + T HG E + RD +E KS
Sbjct: 254 SPLPCRQPMHVVVGKPIEVTKTLKPTDEEIAKFHGQYVE----ALRDLFERHKS 303
>At3g21660 ubiquitin-regulatory protein, putative
Length = 435
Score = 28.1 bits (61), Expect = 9.4
Identities = 24/107 (22%), Positives = 47/107 (43%), Gaps = 5/107 (4%)
Query: 286 GIHSDETVSEHRGNHYPIMHLHERSLSVLACRHVDEVIIGAPWEITKDMITTFNISLVVH 345
G SDE V + G + E+S ++ V + P ++ +T+++
Sbjct: 106 GSDSDEAVEHYGGEENRAIERPEQSSRSMSEETVSSELEEEPQKVFTHTVTSWSNGF--- 162
Query: 346 GTVAEKSLHSERDPYEVPKSMGMFRLLESPKDITTTTVAQRILANHE 392
TV + SL + DP E + + +ESP+++ V +I++ E
Sbjct: 163 -TVDDSSLKTLDDP-ENATFLEIISSMESPRELGQVRVQVKIISREE 207
Database: ara_mips
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,978,382
Number of sequences in database: 6832
Database: /data/blast2/ara_mips_chr2
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,737,135
Number of sequences in database: 4184
Database: /data/blast2/ara_mips_chr3
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,236,886
Number of sequences in database: 5377
Database: /data/blast2/ara_mips_chr4
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 1,748,816
Number of sequences in database: 4030
Database: /data/blast2/ara_mips_chr5
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 2,569,679
Number of sequences in database: 6098
Database: /data/blast2/ara_mips_chl
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 25,951
Number of sequences in database: 85
Database: /data/blast2/ara_mips_mit
Posted date: Jul 15, 2004 10:29 AM
Number of letters in database: 21,747
Number of sequences in database: 113
Lambda K H
0.319 0.137 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,864,661
Number of Sequences: 26719
Number of extensions: 432845
Number of successful extensions: 1025
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 1001
Number of HSP's gapped (non-prelim): 22
length of query: 418
length of database: 11,318,596
effective HSP length: 102
effective length of query: 316
effective length of database: 8,593,258
effective search space: 2715469528
effective search space used: 2715469528
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0004a.6