BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0592.7
(116 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG644690 weakly similar to GP|18542179|gb putative pol protein {... 98 5e-22
AW689768 weakly similar to GP|10177485|d polyprotein {Arabidopsi... 89 3e-19
BG587156 similar to PIR|G85055|G8 probable polyprotein [imported... 82 4e-17
CB893680 weakly similar to GP|1167523|db ORF(AA 1-1338) {Nicotia... 64 7e-12
CB893805 similar to GP|10177935|d copia-type polyprotein {Arabid... 47 4e-11
BG587174 similar to PIR|A47759|A4775 retrovirus-related reverse ... 60 1e-10
BG586293 weakly similar to PIR|E84473|E84 probable retroelement ... 48 5e-07
BI309716 weakly similar to PIR|G96722|G9 hypothetical protein F2... 31 0.061
BQ143821 27 0.88
TC82700 similar to GP|23617236|dbj|BAC20903. hypothetical protei... 25 5.7
BE320454 25 5.7
TC86822 weakly similar to GP|18086336|gb|AAL57631.1 At1g78060/F2... 24 7.5
TC87401 similar to PIR|B84587|B84587 probable glutaredoxin [impo... 24 7.5
BG589079 24 9.8
TC86679 similar to GP|17065046|gb|AAL32677.1 Unknown protein {Ar... 24 9.8
TC90643 weakly similar to GP|19386797|dbj|BAB86176. hypothetical... 24 9.8
TC81375 weakly similar to GP|21537367|gb|AAM61708.1 unknown {Ara... 24 9.8
TC88072 similar to PIR|T06580|T06580 subtilisin-like proteinase ... 24 9.8
>BG644690 weakly similar to GP|18542179|gb putative pol protein {Zea mays},
partial (22%)
Length = 629
Score = 97.8 bits (242), Expect = 5e-22
Identities = 47/108 (43%), Positives = 73/108 (67%)
Frame = -2
Query: 6 FTQKEGIGYFDTYAPVARITTIRVLLALASLYKFVIHQMDVKTAFLNGELDEEVYMKQPE 65
+ QKEGI Y + ++PVAR+ IR+L+A A+ F ++QMDVK+AF+NG+L EEV++KQP
Sbjct: 388 YNQKEGIDYDEAFSPVARMEAIRILIAFAAFMGFKLYQMDVKSAFINGDLKEEVFVKQPP 209
Query: 66 GFVIKGQVQKVCKLTKSLYELKQTPKKKWHQKFDQVVLAHEYKINEFD 113
GF V +L K+LY LKQ P + W+++ + +L + +K + D
Sbjct: 208 GFEDAEVPNHVFRLNKTLYGLKQAP-RAWYERLSKFLLKNGFKRGKID 68
>AW689768 weakly similar to GP|10177485|d polyprotein {Arabidopsis thaliana},
partial (9%)
Length = 675
Score = 88.6 bits (218), Expect = 3e-19
Identities = 45/91 (49%), Positives = 61/91 (66%)
Frame = +1
Query: 6 FTQKEGIGYFDTYAPVARITTIRVLLALASLYKFVIHQMDVKTAFLNGELDEEVYMKQPE 65
F+Q G Y +T++PV + TIR++L +A YK+ I Q+D+ AFLNG L EEVYM QP+
Sbjct: 268 FSQTLGCDYTETFSPVIKPVTIRLILTIAITYKWEIQQIDINNAFLNGFLQEEVYMSQPQ 447
Query: 66 GFVIKGQVQKVCKLTKSLYELKQTPKKKWHQ 96
GF + VCKL KSLY LKQ P + W++
Sbjct: 448 GFEAANK-SLVCKLNKSLYGLKQAP-RAWYE 534
>BG587156 similar to PIR|G85055|G8 probable polyprotein [imported] -
Arabidopsis thaliana, partial (17%)
Length = 618
Score = 81.6 bits (200), Expect = 4e-17
Identities = 44/108 (40%), Positives = 66/108 (60%)
Frame = -1
Query: 6 FTQKEGIGYFDTYAPVARITTIRVLLALASLYKFVIHQMDVKTAFLNGELDEEVYMKQPE 65
FT G Y +T+APVA++ TIR++L+LA + + QMDVK AFL GEL++EVYM P
Sbjct: 351 FTLTYGEDYIETFAPVAKLHTIRIVLSLAVNLGWGLWQMDVKNAFLQGELEDEVYMYPPP 172
Query: 66 GFVIKGQVQKVCKLTKSLYELKQTPKKKWHQKFDQVVLAHEYKINEFD 113
G + V +L K++Y LKQ+P + W+ K + ++ +E D
Sbjct: 171 GLEHLVKRGNVLRLKKAIYGLKQSP-RAWYNKLSTTLNGRGFRKSELD 31
>CB893680 weakly similar to GP|1167523|db ORF(AA 1-1338) {Nicotiana tabacum},
partial (7%)
Length = 780
Score = 64.3 bits (155), Expect = 7e-12
Identities = 34/76 (44%), Positives = 51/76 (66%), Gaps = 1/76 (1%)
Frame = -2
Query: 18 YAPVARITTIRVLLALASLYKFVIHQMDVKTAFLNGELDEEVYMKQPEGFVIKGQVQK-V 76
+ P+ ++ TI LL++ ++ + +DVKTAFL G+L E++YM QPEGF +V K V
Sbjct: 554 FVPIVKLNTIMFLLSIVAIENLYLE*LDVKTAFLRGDLVEDIYMHQPEGF--S*EVGKMV 381
Query: 77 CKLTKSLYELKQTPKK 92
KL KS+Y LKQ P++
Sbjct: 380 GKLKKSMYGLKQGPRQ 333
>CB893805 similar to GP|10177935|d copia-type polyprotein {Arabidopsis
thaliana}, partial (14%)
Length = 778
Score = 46.6 bits (109), Expect(2) = 4e-11
Identities = 20/35 (57%), Positives = 29/35 (82%)
Frame = +2
Query: 41 IHQMDVKTAFLNGELDEEVYMKQPEGFVIKGQVQK 75
++Q+DVK+AFL GEL+EEV++ QP+G+V KG K
Sbjct: 359 VYQLDVKSAFLYGELNEEVFVDQPQGYVKKGDKLK 463
Score = 35.0 bits (79), Expect(2) = 4e-11
Identities = 15/33 (45%), Positives = 25/33 (75%)
Frame = +3
Query: 6 FTQKEGIGYFDTYAPVARITTIRVLLALASLYK 38
++Q+ G+ Y + +APVAR TIR+++ALA+ K
Sbjct: 249 YSQQYGVDYTEVFAPVARWDTIRMVIALAAQIK 347
>BG587174 similar to PIR|A47759|A4775 retrovirus-related reverse
transcriptase homolog - rape retrotransposon copia-like
(fragment), partial (84%)
Length = 249
Score = 60.1 bits (144), Expect = 1e-10
Identities = 26/46 (56%), Positives = 37/46 (79%)
Frame = -1
Query: 54 ELDEEVYMKQPEGFVIKGQVQKVCKLTKSLYELKQTPKKKWHQKFD 99
EL+E++YM QPEGF+ G+ VCKL KSLY LKQ+P ++W+++FD
Sbjct: 249 ELEEKIYMTQPEGFLFPGKEDHVCKLRKSLYGLKQSP-RQWYKRFD 115
>BG586293 weakly similar to PIR|E84473|E84 probable retroelement pol
polyprotein [imported] - Arabidopsis thaliana, partial
(7%)
Length = 763
Score = 48.1 bits (113), Expect = 5e-07
Identities = 24/48 (50%), Positives = 34/48 (70%)
Frame = +2
Query: 6 FTQKEGIGYFDTYAPVARITTIRVLLALASLYKFVIHQMDVKTAFLNG 53
+ +++GI + + +APV RI TI +LLALA+ IH +DVK AFLNG
Sbjct: 179 YVKQQGIDFDEVFAPVVRIETI*LLLALAATNGC*IHHIDVKIAFLNG 322
>BI309716 weakly similar to PIR|G96722|G9 hypothetical protein F20P5.25
[imported] - Arabidopsis thaliana, partial (10%)
Length = 744
Score = 31.2 bits (69), Expect = 0.061
Identities = 13/33 (39%), Positives = 22/33 (66%)
Frame = +2
Query: 75 KVCKLTKSLYELKQTPKKKWHQKFDQVVLAHEY 107
KVC+L KS+Y LKQ ++W+ K + +++ Y
Sbjct: 17 KVCELQKSIYGLKQA-SRQWYSKLSESLISFGY 112
>BQ143821
Length = 810
Score = 27.3 bits (59), Expect = 0.88
Identities = 12/35 (34%), Positives = 18/35 (51%)
Frame = +3
Query: 76 VCKLTKSLYELKQTPKKKWHQKFDQVVLAHEYKIN 110
VC K +Y L P K W Q ++L ++ K+N
Sbjct: 180 VCVRLKHIYILHDQPTKCWCQDLSHMML*YQIKVN 284
>TC82700 similar to GP|23617236|dbj|BAC20903. hypothetical protein~similar
to Arabidopsis thaliana chromosome 4 At4g13970, partial
(39%)
Length = 1066
Score = 24.6 bits (52), Expect = 5.7
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = +2
Query: 94 WHQKFDQVVLAHEYKINEFDK 114
WH K D+V L ++N+F K
Sbjct: 134 WHDKLDRVALIPFARVNDFVK 196
>BE320454
Length = 338
Score = 24.6 bits (52), Expect = 5.7
Identities = 14/47 (29%), Positives = 22/47 (46%)
Frame = -1
Query: 13 GYFDTYAPVARITTIRVLLALASLYKFVIHQMDVKTAFLNGELDEEV 59
G F+ ++ A TT + L S + +Q AFLN +DE +
Sbjct: 275 GSFEPHSISASFTTCSLSLVWFSFFAKAPYQTSNPAAFLNKPIDESM 135
>TC86822 weakly similar to GP|18086336|gb|AAL57631.1 At1g78060/F28K19_32
{Arabidopsis thaliana}, partial (8%)
Length = 704
Score = 24.3 bits (51), Expect = 7.5
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = +1
Query: 28 RVLLALASLYKFVIHQMDVKTAFLNGELDEEVYM 61
RVL SL K I ++ +K NG+LDE + M
Sbjct: 457 RVLN*YHSLMKCSIEKLSIK*RITNGKLDELIAM 558
>TC87401 similar to PIR|B84587|B84587 probable glutaredoxin [imported] -
Arabidopsis thaliana, partial (63%)
Length = 914
Score = 24.3 bits (51), Expect = 7.5
Identities = 14/38 (36%), Positives = 21/38 (54%)
Frame = +2
Query: 53 GELDEEVYMKQPEGFVIKGQVQKVCKLTKSLYELKQTP 90
GEL Y QPEG +I+ + ++C L +L+ TP
Sbjct: 590 GELRPCQYYSQPEGNLIRLPICRICWL-----KLESTP 688
>BG589079
Length = 385
Score = 23.9 bits (50), Expect = 9.8
Identities = 11/33 (33%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
Frame = -1
Query: 65 EGFVIKGQVQKVCKLTKSLYELKQTPKKK-WHQ 96
+GF++ K+C L+K L +K P+++ W Q
Sbjct: 292 KGFLVARVN*KICMLSKHLLSMKCIPEER*WKQ 194
>TC86679 similar to GP|17065046|gb|AAL32677.1 Unknown protein {Arabidopsis
thaliana}, partial (48%)
Length = 1445
Score = 23.9 bits (50), Expect = 9.8
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = -2
Query: 72 QVQKVCKLTKSLYELKQTPKKKWHQKFDQVVLAHEY 107
Q+Q + + SLY+L P K+ Q DQ +L+ +
Sbjct: 433 QIQNLLQ*LFSLYDLPLKPLKERSQLHDQFLLSQNH 326
>TC90643 weakly similar to GP|19386797|dbj|BAB86176. hypothetical
protein~similar to Arabidopsis thaliana protein
T1F15.13, partial (21%)
Length = 771
Score = 23.9 bits (50), Expect = 9.8
Identities = 9/25 (36%), Positives = 18/25 (72%), Gaps = 1/25 (4%)
Frame = +3
Query: 85 ELKQTPKKKWHQKFDQVV-LAHEYK 108
+L++ PK+KW + + QV ++H Y+
Sbjct: 90 QLRKVPKQKWTEMWRQVKNISHHYE 164
>TC81375 weakly similar to GP|21537367|gb|AAM61708.1 unknown {Arabidopsis
thaliana}, partial (30%)
Length = 797
Score = 23.9 bits (50), Expect = 9.8
Identities = 8/23 (34%), Positives = 13/23 (55%)
Frame = -2
Query: 83 LYELKQTPKKKWHQKFDQVVLAH 105
++++ Q WHQ DQV+ H
Sbjct: 298 VHQMLQLSCNHWHQNLDQVIPLH 230
>TC88072 similar to PIR|T06580|T06580 subtilisin-like proteinase (EC
3.4.21.-) p69f - tomato, partial (27%)
Length = 1097
Score = 23.9 bits (50), Expect = 9.8
Identities = 11/27 (40%), Positives = 15/27 (54%), Gaps = 4/27 (14%)
Frame = +1
Query: 93 KWH----QKFDQVVLAHEYKINEFDKC 115
KW+ QKF ++A YKI+ D C
Sbjct: 313 KWYCCIDQKFSP*LVASRYKISNHDNC 393
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.328 0.143 0.426
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,099,692
Number of Sequences: 36976
Number of extensions: 33600
Number of successful extensions: 212
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 210
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 210
length of query: 116
length of database: 9,014,727
effective HSP length: 92
effective length of query: 24
effective length of database: 5,612,935
effective search space: 134710440
effective search space used: 134710440
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 50 (23.9 bits)
Lotus: description of TM0592.7