BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0590c.5
(441 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG647389 69 2e-17
BE999414 59 4e-09
BG448304 40 0.001
TC89839 homologue to SP|P23466|CYAA_SACKL Adenylate cyclase (EC ... 40 0.002
TC77786 37 0.020
TC76514 homologue to PIR|T09648|T09648 nucleolin homolog nuM1 - ... 35 0.058
TC76531 similar to SP|P13915|CVCA_PEA Convicilin precursor. [Gar... 35 0.075
TC91757 homologue to GP|7339534|emb|CAB82852.1 protein kinase MK... 33 0.22
BF006363 similar to GP|23496060|gb hypothetical protein {Plasmod... 33 0.29
CB065382 similar to PIR|T09671|T096 RPE15 protein - alfalfa (fra... 33 0.29
TC86770 similar to GP|13872875|dbj|BAB43982. contains EST AU0965... 32 0.64
TC81207 similar to GP|21322752|dbj|BAB78536. cold shock protein-... 31 0.83
TC81816 similar to GP|8096269|dbj|BAA95789.1 KED {Nicotiana taba... 31 1.1
BG451613 30 1.4
BG644741 30 1.9
TC83625 similar to GP|8096269|dbj|BAA95789.1 KED {Nicotiana taba... 30 1.9
TC93668 similar to PIR|T52393|T52393 hypothetical protein MMB12.... 30 1.9
TC77144 similar to GP|20160594|dbj|BAB89541. contains ESTs AU031... 30 2.4
BE941219 weakly similar to GP|10177251|db receptor protein kinas... 29 3.2
TC84161 similar to GP|15291849|gb|AAK93193.1 LD29569p {Drosophil... 29 3.2
>BG647389
Length = 718
Score = 68.6 bits (166), Expect(2) = 2e-17
Identities = 33/85 (38%), Positives = 54/85 (62%)
Frame = +1
Query: 49 KSTAMAWFTTLPRGSISNFRDFSSKFLVQFSANKNQPVTINDLYNIRQQEGESLKEYMAR 108
K W+ LPR SI+ +++ + K + QFSA+K+ V+ +L+N+RQ ESL EY+
Sbjct: 451 KEATQQWYMNLPRFSITGYQNMTHKLVHQFSASKHCKVSTTNLFNVRQDPNESLPEYLV* 630
Query: 109 YSAASVKVEDEEPRACALAFKNGLL 133
++ A++KV + + A AF+NGLL
Sbjct: 631 FNNATIKVVNPKQELFAGAFQNGLL 705
Score = 38.5 bits (88), Expect(2) = 2e-17
Identities = 16/39 (41%), Positives = 26/39 (66%)
Frame = +2
Query: 3 DNMKTLVLDSYSGDSDPKDHLLYFNTKMVIIAASDAVKC 41
+N+K+L L S+ SDP +H FNT+M +I A +++C
Sbjct: 317 ENLKSLSLLSFDDKSDPVEHATTFNTQMAVIGALKSLRC 433
>BE999414
Length = 613
Score = 58.9 bits (141), Expect = 4e-09
Identities = 26/76 (34%), Positives = 41/76 (53%)
Frame = +3
Query: 10 LDSYSGDSDPKDHLLYFNTKMVIIAASDAVKCRMFPSTFKSTAMAWFTTLPRGSISNFRD 69
+DSY G DP +H+ + + AVKC++F +T + AM WF L R SI ++ D
Sbjct: 9 MDSYDGT*DPDEHMENIEVVLTYRSVRGAVKCKLFVTTLRRGAMTWFKNLRRNSIGSWGD 188
Query: 70 FSSKFLVQFSANKNQP 85
+F F+ ++ QP
Sbjct: 189LCHEFTTHFTVSRTQP 236
>BG448304
Length = 637
Score = 40.4 bits (93), Expect = 0.001
Identities = 31/117 (26%), Positives = 50/117 (42%), Gaps = 8/117 (6%)
Frame = +2
Query: 264 PEAPKYQPRDANPKKWCEFHRSAGHDTDDCWTLQREIDKLIRAGYQGNRQGQWRNGGDQN 323
P+ P + + + K+C FH+ H TDDC L+ I+ LI+ G N +N
Sbjct: 65 PKTPTQENKGTDKTKYCRFHKCHRHLTDDCIHLKDTIEILIQRGR--------LNQLTKN 220
Query: 324 KAHKREEERADTKGKKKQESAAIATKGADDTFARH--------SGPPVGTINTIAGG 372
+++ + T K K A++ + D+ F H + P T N I GG
Sbjct: 221 PEPEKQTVKLITDKKNKDIVVAMSVEQLDE-FVEHVDITPYSCTWEPFPTANVITGG 388
>TC89839 homologue to SP|P23466|CYAA_SACKL Adenylate cyclase (EC 4.6.1.1)
(ATP pyrophosphate-lyase) (Adenylyl cyclase). [Yeast],
partial (0%)
Length = 665
Score = 40.0 bits (92), Expect = 0.002
Identities = 34/113 (30%), Positives = 48/113 (42%), Gaps = 7/113 (6%)
Frame = +3
Query: 199 QQRPGKGKSVFKPTK-EQLYPRRDDYEQRRPWQSKSHRQREETDMVMNTDV--SDMLRGA 255
QQRP P K Q PR+ + + P + Q ++ V +D+L
Sbjct: 240 QQRPQSPYQPMCPQKPRQQAPRQSIPQNQVPQKFIPQNQVQKASQCDPIPVKYADLLPIL 419
Query: 256 SDANLVDE---PEAPKYQPRDANPKKWCEFHRSA-GHDTDDCWTLQREIDKLI 304
NL+ P P P P C FH+ A GHDT+ C+ L+ E+ KLI
Sbjct: 420 LKKNLIQTLPLPRVPNSLPPWYRPDLNCVFHQGAPGHDTEQCYPLKEEVQKLI 578
>TC77786
Length = 1094
Score = 36.6 bits (83), Expect = 0.020
Identities = 17/46 (36%), Positives = 29/46 (62%)
Frame = +3
Query: 260 LVDEPEAPKYQPRDANPKKWCEFHRSAGHDTDDCWTLQREIDKLIR 305
+ ++P+ K R + K C +HRS GHDT++C+ L+ I+ LI+
Sbjct: 768 IANKPQLLK*SAR-TDKAK*CCYHRSRGHDTEECFQLREAIEDLIK 902
>TC76514 homologue to PIR|T09648|T09648 nucleolin homolog nuM1 - alfalfa,
partial (61%)
Length = 1288
Score = 35.0 bits (79), Expect = 0.058
Identities = 42/165 (25%), Positives = 69/165 (41%), Gaps = 8/165 (4%)
Frame = +3
Query: 117 EDEEPRACALAFKNGLLPGGLNSKLTRKPARSMGEMRARASTYILDEEDEAFKRKRAKLE 176
EDE+P A A+ KNG +P +K + + +S DEED K+ AK
Sbjct: 669 EDEKPAAKAVPSKNGSVP-------AKKADTESSDEDSESS----DEED---KKPAAKAS 806
Query: 177 KGDTSPKRVKKDRSGEDKGDGKQQRPGKGKSVFKPTKEQLYPRRD-------DYEQRRPW 229
K ++P + K S +++ D + K V KP ++D D +
Sbjct: 807 KNVSAPTK-KAASSSDEESDEESDEDEDAKPVSKPAAVAKKSKKDSSDSDDEDDDSSSDE 983
Query: 230 QSKSHRQREETDMVMNTDVSDMLRGASDANLVDEP-EAPKYQPRD 273
K ++E M ++ D SD S+ DEP + P+ + +D
Sbjct: 984 DKKPVAAKKEDKMNVDKDGSD--SDQSEEESEDEPSKTPQKKIKD 1112
>TC76531 similar to SP|P13915|CVCA_PEA Convicilin precursor. [Garden pea]
{Pisum sativum}, partial (86%)
Length = 2217
Score = 34.7 bits (78), Expect = 0.075
Identities = 35/167 (20%), Positives = 72/167 (42%)
Frame = +2
Query: 176 EKGDTSPKRVKKDRSGEDKGDGKQQRPGKGKSVFKPTKEQLYPRRDDYEQRRPWQSKSHR 235
E+ + P + R G +G++QRPG+ ++P+ E+ D++++RRP Q + R
Sbjct: 161 EEDERRPGEWRPPRGGRQ--EGQEQRPGQ----WRPSHEREEDEEDEHQKRRPGQWRPTR 322
Query: 236 QREETDMVMNTDVSDMLRGASDANLVDEPEAPKYQPRDANPKKWCEFHRSAGHDTDDCWT 295
+ R S+ DE + + +P P + E + W
Sbjct: 323 GGRQEGQEQRPG-----RFPSEREEYDEDDRRERRPGQQRPTR--EGRWKGQEQRPEQWR 481
Query: 296 LQREIDKLIRAGYQGNRQGQWRNGGDQNKAHKREEERADTKGKKKQE 342
R ++ + Q ++ G+ ++ K KRE+E + +G+++ E
Sbjct: 482 PSRGKEEREKEERQKHQPGR-----EREKWEKREDE--EWRGRQRHE 601
>TC91757 homologue to GP|7339534|emb|CAB82852.1 protein kinase MK6
{Mesembryanthemum crystallinum}, partial (36%)
Length = 791
Score = 33.1 bits (74), Expect = 0.22
Identities = 23/98 (23%), Positives = 45/98 (45%)
Frame = +2
Query: 162 DEEDEAFKRKRAKLEKGDTSPKRVKKDRSGEDKGDGKQQRPGKGKSVFKPTKEQLYPRRD 221
D+E ++ + +KL+ D K K+D G + G+G P S+ TK+++ +
Sbjct: 80 DKEMDSARSWLSKLQPRDKM-KAKKRDEDGNNDGNGDLTSPVDDGSLSDVTKQKVAAAKQ 256
Query: 222 DYEQRRPWQSKSHRQREETDMVMNTDVSDMLRGASDAN 259
E Q K+ ++R+E ++ ++D D N
Sbjct: 257 YIENHYKEQMKNLQERKERRTILEKKLADAEVSEEDQN 370
>BF006363 similar to GP|23496060|gb hypothetical protein {Plasmodium
falciparum 3D7}, partial (0%)
Length = 583
Score = 32.7 bits (73), Expect = 0.29
Identities = 15/35 (42%), Positives = 22/35 (62%), Gaps = 2/35 (5%)
Frame = -1
Query: 278 KWCEFHRSAGHDTDDCWTLQ--REIDKLIRAGYQG 310
K+C FHR G+DT+DC+ + +E K + YQG
Sbjct: 499 KYCRFHRCTGYDTNDCFQ*EAIKESIKKRKPKYQG 395
>CB065382 similar to PIR|T09671|T096 RPE15 protein - alfalfa (fragment),
partial (9%)
Length = 624
Score = 32.7 bits (73), Expect = 0.29
Identities = 17/46 (36%), Positives = 25/46 (53%), Gaps = 1/46 (2%)
Frame = -2
Query: 263 EPEAPKYQPRDANPKKWCEFHRSA-GHDTDDCWTLQREIDKLIRAG 307
+P PR + K C+FH+ A GHD + C+ L+ + KLI G
Sbjct: 398 KPPPDPLPPRFRSDLK-CDFHQGALGHDVEGCYALKHIVKKLINQG 264
>TC86770 similar to GP|13872875|dbj|BAB43982. contains EST
AU096506(S14064)~unknown protein {Oryza sativa (japonica
cultivar-group)}, partial (73%)
Length = 2061
Score = 31.6 bits (70), Expect = 0.64
Identities = 28/114 (24%), Positives = 45/114 (38%), Gaps = 2/114 (1%)
Frame = +2
Query: 161 LDEEDEAFKRKRAKLEKGDTSPKRVKKDRSGEDKGDGK--QQRPGKGKSVFKPTKEQLYP 218
L+EEDE KRK+ K KR+K S KG + + G G + ++
Sbjct: 380 LEEEDEGRKRKKGKDGGSGKKEKRLKGGSSSSGKGGSRFGGSKRGVGGKSGNDREGEVNE 559
Query: 219 RRDDYEQRRPWQSKSHRQREETDMVMNTDVSDMLRGASDANLVDEPEAPKYQPR 272
D ++ R ++ + + +T V L G DEP +P P+
Sbjct: 560 MWDALAGNSEDDNEGARNMDDDNFIDDTGVEPALYG------YDEPRSPGDAPQ 703
>TC81207 similar to GP|21322752|dbj|BAB78536. cold shock protein-1 {Triticum
aestivum}, partial (39%)
Length = 630
Score = 31.2 bits (69), Expect = 0.83
Identities = 24/85 (28%), Positives = 34/85 (39%), Gaps = 14/85 (16%)
Frame = +3
Query: 307 GYQGNRQGQWRNGG--------------DQNKAHKREEERADTKGKKKQESAAIATKGAD 352
G +G R G+ RNGG D +++ + + G A T G+
Sbjct: 303 GGRGFRGGERRNGGGGCYTCGDTGHIARDCDRSDRNDRNDRSGGGGGGDRDRACYTCGSF 482
Query: 353 DTFARHSGPPVGTINTIAGGFGGGG 377
+ FAR G N GG+GGGG
Sbjct: 483 EHFARDCMRGGGNNNNGGGGYGGGG 557
>TC81816 similar to GP|8096269|dbj|BAA95789.1 KED {Nicotiana tabacum},
partial (13%)
Length = 663
Score = 30.8 bits (68), Expect = 1.1
Identities = 25/106 (23%), Positives = 45/106 (41%)
Frame = +3
Query: 136 GLNSKLTRKPARSMGEMRARASTYILDEEDEAFKRKRAKLEKGDTSPKRVKKDRSGEDKG 195
G + K K + E + DE+ + K+K+ K EKG + KD+ GE+K
Sbjct: 234 GKDKKDKEKKKKEKKEENVKGEEEDGDEKKDKEKKKKEKKEKG-----KEDKDKDGEEKK 398
Query: 196 DGKQQRPGKGKSVFKPTKEQLYPRRDDYEQRRPWQSKSHRQREETD 241
K + K K+ E D +++ + K +++EE +
Sbjct: 399 SKKDKEKKKDKNEDDDEGE-------DGSKKKKNKDKKEKKKEEDE 515
>BG451613
Length = 636
Score = 30.4 bits (67), Expect = 1.4
Identities = 34/122 (27%), Positives = 47/122 (37%), Gaps = 2/122 (1%)
Frame = +3
Query: 173 AKLEKGDTSPKRVKKDRSGEDKGDGKQQRP-GKGKSVFKPTKEQLYPRRDDYEQRRPWQS 231
A L D + + +D S ED+ DGK P K V K + DD P+
Sbjct: 186 AGLAYDDKTSELDVQDESQEDRQDGKMTIPLNDDKDV--QGKTTILASDDD-----PFDL 344
Query: 232 KSHRQREETDMVMNTDVSDMLRGASD-ANLVDEPEAPKYQPRDANPKKWCEFHRSAGHDT 290
K+ EE D + TD ++L S+ AN + + E DA HD
Sbjct: 345 KNSFAGEEEDNISETDKKNLLEDTSEHANEISQEENQNIPQEDA-------------HDA 485
Query: 291 DD 292
DD
Sbjct: 486 DD 491
>BG644741
Length = 735
Score = 30.0 bits (66), Expect = 1.9
Identities = 18/70 (25%), Positives = 30/70 (42%)
Frame = -2
Query: 42 RMFPSTFKSTAMAWFTTLPRGSISNFRDFSSKFLVQFSANKNQPVTINDLYNIRQQEGES 101
R+FP + A WFT LP SI + FL ++ + + + N GES
Sbjct: 584 RVFPLSLMGEADIWFTELPYNSIFTWNQLRDVFLARYYPVSKKLNHNDRVNNFVALPGES 405
Query: 102 LKEYMARYSA 111
+ R+++
Sbjct: 404 VSSSWDRFTS 375
>TC83625 similar to GP|8096269|dbj|BAA95789.1 KED {Nicotiana tabacum},
partial (9%)
Length = 908
Score = 30.0 bits (66), Expect = 1.9
Identities = 20/72 (27%), Positives = 32/72 (43%)
Frame = +2
Query: 136 GLNSKLTRKPARSMGEMRARASTYILDEEDEAFKRKRAKLEKGDTSPKRVKKDRSGEDKG 195
G + K K + E + DE+ + K+K+ K EKG + KD+ GE+K
Sbjct: 626 GKDKKDKEKKKKEKKEENVKGEEEDGDEKKDKEKKKKEKKEKG-----KEDKDKDGEEKK 790
Query: 196 DGKQQRPGKGKS 207
K + K K+
Sbjct: 791 SKKDKEKKKDKN 826
>TC93668 similar to PIR|T52393|T52393 hypothetical protein MMB12.12
[imported] - Arabidopsis thaliana, partial (10%)
Length = 640
Score = 30.0 bits (66), Expect = 1.9
Identities = 22/78 (28%), Positives = 40/78 (51%), Gaps = 6/78 (7%)
Frame = +3
Query: 169 KRKRAKLEKGDTSPKRVKKDRSGEDKGDGKQQRPGKGKSVFKPTKEQLYPR------RDD 222
KR+ +KL++ + SPK+ ++DR E P K S +P + + Y + + +
Sbjct: 96 KRRHSKLDR-EPSPKKYRRDRERERLTTNGHSEPSKHSS--QPPRSRSYYQVGRSTGQRE 266
Query: 223 YEQRRPWQSKSHRQREET 240
+++ QSK + QR ET
Sbjct: 267 ADEKVYAQSKENNQRLET 320
>TC77144 similar to GP|20160594|dbj|BAB89541. contains ESTs AU031508(E61760)
AU068322(C20031) AU068323(C20031)~similar to Arabidopsis
thaliana, partial (46%)
Length = 817
Score = 29.6 bits (65), Expect = 2.4
Identities = 36/166 (21%), Positives = 68/166 (40%), Gaps = 16/166 (9%)
Frame = +3
Query: 203 GKGKSVFKPTKEQLYPRRDDYEQRRPWQSKSHRQRE-ETDMVMNTDVSD----------- 250
G+GK KPT + + +D + K+ RQ+E E + +VS+
Sbjct: 114 GRGKFKAKPTGRRNFSTHEDMVAGTSSRPKTFRQKEPENEDEATREVSEDESEEESEDEN 293
Query: 251 -MLRGASDANLVDEPEAPK---YQPRDANPKKWCEFHRSAGHDTDDCWTLQREIDKLIRA 306
+G ++ P K + RD + K E R + + QR ++ ++
Sbjct: 294 TKTKGVQGVIEIENPNLVKPKNIKARDIDIGKTSELSRREREEIEK----QRAHERYMKL 461
Query: 307 GYQGNRQGQWRNGGDQNKAHKREEERADTKGKKKQESAAIATKGAD 352
QG + ++ D ++ +ERA+ K+++E AA K A+
Sbjct: 462 QEQGKTE---QSRKDLDRLALIRQERAEAAKKREEEKAAKEQKKAE 590
>BE941219 weakly similar to GP|10177251|db receptor protein kinase-like
protein {Arabidopsis thaliana}, partial (7%)
Length = 586
Score = 29.3 bits (64), Expect = 3.2
Identities = 18/75 (24%), Positives = 31/75 (41%), Gaps = 5/75 (6%)
Frame = -1
Query: 32 IIAASDAVKCRMFPSTFKSTAMAWFTTLPR-----GSISNFRDFSSKFLVQFSANKNQPV 86
+ A++ C+MFP+TF + WF R +++ + K L + N +
Sbjct: 340 LACATNCSNCKMFPNTFGISPEKWFAETTRDFRLVSLLNSLLNLPEKLLAESDNLSNDNI 161
Query: 87 TINDLYNIRQQEGES 101
ND + E ES
Sbjct: 160 EYNDSGKGPENELES 116
>TC84161 similar to GP|15291849|gb|AAK93193.1 LD29569p {Drosophila
melanogaster}, partial (3%)
Length = 759
Score = 29.3 bits (64), Expect = 3.2
Identities = 31/111 (27%), Positives = 43/111 (37%), Gaps = 24/111 (21%)
Frame = -3
Query: 121 PRACALAFKNGLLPGGLNSKLTR-----KPARSMGEMRARASTYI--LDEEDEAFKRKRA 173
P LA LPG + L K GEM+ + LD E + RA
Sbjct: 532 PPPAKLAPPRSYLPGRGHGPLVEIIASAKSKADGGEMKILNKEELEKLDLSPELVEEMRA 353
Query: 174 KLEK---------------GDTSPKRVKK--DRSGEDKGDGKQQRPGKGKS 207
+LEK GD P+ ++ D SGE GDG +++ GK+
Sbjct: 352 ELEKIAGDKGWKLEVIEQPGDGDPREIRGTVDHSGEKGGDGGREKGEDGKA 200
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.315 0.132 0.387
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,919,755
Number of Sequences: 36976
Number of extensions: 129545
Number of successful extensions: 916
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 905
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 916
length of query: 441
length of database: 9,014,727
effective HSP length: 99
effective length of query: 342
effective length of database: 5,354,103
effective search space: 1831103226
effective search space used: 1831103226
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.5 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0590c.5