TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0588b.10
         (328 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

CB895143                                                               65  4e-11
TC82834 homologue to PIR|B85078|B85078 probable glucosyltransfer...    31  0.75
TC82180 similar to GP|15010770|gb|AAK74044.1 AT5g57120/MUL3_6 {A...    28  3.7
TC84395 similar to GP|11385579|gb|AAG34872.1 In2-1 protein {Glyc...    28  4.9
BF643397 homologue to GP|22770980|gb G protein alpha I subunit {...    28  6.4
TC85548 similar to PIR|T51854|T51854 RING-H2 finger protein RHF2...    27  8.3
TC87690 similar to GP|15028227|gb|AAK76610.1 putative oligopepti...    27  8.3
TC82087 homologue to GP|14324365|dbj|BAB59293. hypothetical prot...    27  8.3

>CB895143 
          Length = 770

 Score = 65.1 bits (157), Expect = 4e-11
 Identities = 44/182 (24%), Positives = 83/182 (45%), Gaps = 5/182 (2%)
 Frame = +2

Query: 62  TKWSGKDNILLLQSWLNVSTDRVGGNEQKSDLFWNKIRAQYEEYRD-----DASPSRTWL 116
           + W+   N++L+  W+   T+ V G  QK D +  KI   + E+       DA+P R   
Sbjct: 203 SNWNIDQNLILISGWIKYGTNSVVGRNQKCDSY*GKIVDYFNEHCSFNPPCDAAPCR--- 373

Query: 117 SLKSHFNKLNADLQKFVGCHTKAVNHWKSGHSDKDIMATARQLYHVDTGKDFKHENEWRL 176
              +H+N +N  L K++  +  A    +SG S  D++A A +LY     + F   + W +
Sbjct: 374 ---NHYNYMNKILNKWIDAYDGAKRMQQSG*SKIDVLAKAHELYSSGKSEHFILISGWFV 544

Query: 177 VKDEPKWKGTFMTTSSTRQKKSGDGVYATSSDRSASIKGDEYEATQPATRPLGKKNQKRK 236
           V+D+P++           Q     G  ++ S R+      +  +   ++ P+G+   K++
Sbjct: 545 VRDQPRYDS---------QVGGNTGSGSSGSKRAHKSDASDSNSVGSSSCPMGRDAAKKR 697

Query: 237 AK 238
            K
Sbjct: 698 *K 703


>TC82834 homologue to PIR|B85078|B85078 probable glucosyltransferase
           [imported] - Arabidopsis thaliana, partial (29%)
          Length = 628

 Score = 30.8 bits (68), Expect = 0.75
 Identities = 15/35 (42%), Positives = 20/35 (56%)
 Frame = -3

Query: 8   QYCMYPPQYQAPPTGSSSSSKVSSTQCEAMPEEPE 42
           Q C YPPQ+ + PT   +SSK    Q  A+P  P+
Sbjct: 581 QQCPYPPQWSS-PTNHQASSKAFILQTPAVPLNPK 480


>TC82180 similar to GP|15010770|gb|AAK74044.1 AT5g57120/MUL3_6 {Arabidopsis
           thaliana}, partial (33%)
          Length = 567

 Score = 28.5 bits (62), Expect = 3.7
 Identities = 13/54 (24%), Positives = 27/54 (49%)
 Frame = +3

Query: 118 LKSHFNKLNADLQKFVGCHTKAVNHWKSGHSDKDIMATARQLYHVDTGKDFKHE 171
           +K+ F ++  D  +FV    +  ++W    ++    A A ++     G+DF+HE
Sbjct: 33  VKAAFQRVQVDKVEFVDERLQDNSYWAKDGAESGYGAKAEEILGQVRGRDFRHE 194


>TC84395 similar to GP|11385579|gb|AAG34872.1 In2-1 protein {Glycine max},
           partial (54%)
          Length = 692

 Score = 28.1 bits (61), Expect = 4.9
 Identities = 16/50 (32%), Positives = 27/50 (54%), Gaps = 3/50 (6%)
 Frame = +2

Query: 105 YRDDASPSRTWLSLKSHFNKLNA---DLQKFVGCHTKAVNHWKSGHSDKD 151
           Y+D   P+    SL+ H N++     DL K++  H +  + + SGH DK+
Sbjct: 476 YKDKVHPTNKVPSLE-HNNEVRGESLDLIKYIDTHFEGPSLYPSGHDDKE 622


>BF643397 homologue to GP|22770980|gb G protein alpha I subunit {Pisum
           sativum}, partial (38%)
          Length = 531

 Score = 27.7 bits (60), Expect = 6.4
 Identities = 25/95 (26%), Positives = 39/95 (40%), Gaps = 15/95 (15%)
 Frame = +2

Query: 110 SPSRTWLSLKSHFNKLNADLQKFVGCHTKAVNHWK------SGHSDKDIMATARQLYHVD 163
           S SR +    +  N   A++++ +   TKA  H +      +G S K  +    +L    
Sbjct: 77  SKSRRYNDANTEENAQTAEIERRIELETKAEKHIQKLLLLGAGESGKSTIFKQIKLLF-Q 253

Query: 164 TGKDFK---------HENEWRLVKDEPKWKGTFMT 189
           TG D           H N ++ +K    WKG FMT
Sbjct: 254 TGFDEAELKSYLPVIHANVYQTIKVRNTWKGYFMT 358


>TC85548 similar to PIR|T51854|T51854 RING-H2 finger protein RHF2a
           [imported] - Arabidopsis thaliana, partial (69%)
          Length = 1554

 Score = 27.3 bits (59), Expect = 8.3
 Identities = 36/157 (22%), Positives = 59/157 (36%), Gaps = 17/157 (10%)
 Frame = +3

Query: 2   SYPNNPQYCMYPPQYQAPPTGSSSSS---KVSSTQCEAMPEEPEFSTQRGLDDIDLEESG 58
           S    PQY ++     +PP   +SSS   +    Q  A  EEP   T       D   + 
Sbjct: 582 SAQGRPQYMVFSAHPNSPPMAPASSSPPQRGDGEQTPATGEEPPQLTLVPPVQTDQVSAS 761

Query: 59  KKRTKWSGKDNILLLQSWLN-------VSTDRVGGNEQK--SDLFWNKIRAQYEEYRDDA 109
              +     DN+ L  +           S DR G +E +  S+   +K+ A    Y++  
Sbjct: 762 GSGSAALATDNLGLSYNSRRSPNQSSPSSQDRAGPSELQSFSESLKSKLNAVSTRYKESI 941

Query: 110 SPS-----RTWLSLKSHFNKLNADLQKFVGCHTKAVN 141
           S S       W S  S  + L +++++ V      V+
Sbjct: 942 SKSTRGWKERWFSRTSPMSDLGSEVKREVNAGIATVS 1052


>TC87690 similar to GP|15028227|gb|AAK76610.1 putative oligopeptidase A
           {Arabidopsis thaliana}, partial (30%)
          Length = 1019

 Score = 27.3 bits (59), Expect = 8.3
 Identities = 38/186 (20%), Positives = 65/186 (34%), Gaps = 2/186 (1%)
 Frame = +2

Query: 97  KIRAQYEEYRDDASPSRTWLSLKSHFNKLNADLQKFVGC--HTKAVNHWKSGHSDKDIMA 154
           K+    EE      PS  W  L     K+   L    G   H KAV       S  + + 
Sbjct: 368 KLERDLEELERSVEPS--WPKLVEPLEKIVDRLAVVWGMVNHLKAVKDNSELRSAIEDVQ 541

Query: 155 TARQLYHVDTGKDFKHENEWRLVKDEPKWKGTFMTTSSTRQKKSGDGVYATSSDRSASIK 214
             +  + +  G+     N ++ ++D P WK     T S  +K+  +     +     S++
Sbjct: 542 AEKVKFQLRLGQSKPLYNAFKAIQDSPDWK-----TLSDARKRIVENQIKEAVLNGVSLE 706

Query: 215 GDEYEATQPATRPLGKKNQKRKAKVGDTASSDLDYVPNSEMIAIGKAKLGFLASFEKLKT 274
            D+ E      + L + ++K                        G+  L     FEKL T
Sbjct: 707 DDKREQFNKIEQELERLSEK-----------------------FGENVLDATKKFEKLIT 817

Query: 275 EKLEVK 280
           +K E++
Sbjct: 818 DKKEIE 835


>TC82087 homologue to GP|14324365|dbj|BAB59293. hypothetical protein
           {Thermoplasma volcanium}, partial (3%)
          Length = 514

 Score = 27.3 bits (59), Expect = 8.3
 Identities = 18/58 (31%), Positives = 31/58 (53%), Gaps = 2/58 (3%)
 Frame = -2

Query: 241 DTASSDLDYVPNSEMIAIGKAKLGFLASFEKLKTEKLEVKKEKNKLQK--ARLLKEYK 296
           D+  + ++ + NS++      KL       K++ EKLE++ + N LQK   +L  EYK
Sbjct: 471 DSKITKMENLKNSKISDEKSRKLKLQQILNKIQREKLELENKINILQKKIRKLENEYK 298


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.310    0.127    0.365 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,031,999
Number of Sequences: 36976
Number of extensions: 136356
Number of successful extensions: 666
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 660
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 664
length of query: 328
length of database: 9,014,727
effective HSP length: 96
effective length of query: 232
effective length of database: 5,465,031
effective search space: 1267887192
effective search space used: 1267887192
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 58 (26.9 bits)