BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0546.8
(898 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF648394 59 5e-17
TC82456 weakly similar to GP|18057159|gb|AAL58182.1 putative far... 70 4e-12
AW687028 weakly similar to GP|9909176|dbj| hypothetical protein~... 66 7e-11
BG449141 similar to GP|15983442|gb At1g10240/F14N23_12 {Arabidop... 49 1e-05
TC83834 similar to PIR|G96565|G96565 F6D8.26 [imported] - Arabid... 44 2e-04
TC89654 weakly similar to PIR|T05644|T05644 hypothetical protein... 41 0.002
TC90685 weakly similar to GP|11994595|dbj|BAB02650. receptor-lik... 31 1.8
TC90266 similar to SP|Q9C519|WRK6_ARATH WRKY transcription facto... 31 2.4
TC76791 similar to GP|21311555|gb|AAM46778.1 15.8 kDa oleosin {T... 31 2.4
TC88799 similar to PIR|C71408|C71408 probable protein kinase - A... 30 5.4
TC87453 similar to GP|22093671|dbj|BAC06965. similar to bHLH (Ba... 30 5.4
TC83532 similar to PIR|T38639|T38639 proteasome component PUP2 h... 30 5.4
TC88624 similar to PIR|S51529|S51529 SPF1 protein - sweet potato... 30 5.4
TC77596 similar to GP|18377702|gb|AAL67001.1 putative clathrin b... 30 5.4
BG452313 homologue to PIR|T40261|T40 ubi4 protein - fission yeas... 30 5.4
TC77278 similar to GP|11493822|gb|AAG35658.1 transcription facto... 29 7.0
TC93897 similar to PIR|D86320|D86320 hypothetical protein AAF271... 29 7.0
TC89563 similar to GP|15865943|gb|AAL10121.1 NADH dehydrogenase ... 29 7.0
TC77547 similar to PIR|T48354|T48354 hypothetical protein F12E4.... 29 7.0
>BF648394
Length = 650
Score = 58.5 bits (140), Expect(2) = 5e-17
Identities = 41/118 (34%), Positives = 59/118 (49%), Gaps = 4/118 (3%)
Frame = -3
Query: 405 VEGAHSKLKKLLSDSKGDLVIAWTAMNKLIIMQHDKIKESFQLSIFVTEHSLN-DPFYKH 463
VE A LKK L +S GDL W ++ ++++Q I+ +F S+ V EH Y
Sbjct: 648 VESAXDLLKKYLDNSVGDLGTCWEKIHDMLLLQFIAIQTTFGQSVSVLEHRFKYVTLYSG 469
Query: 464 LRGFVSRAALHIIVKEKTQI-GMIGVGGIYCECVLRRTHGLPCACELMK--FESVPLL 518
+ G VSR AL I E+T + + CV R ++GLPC CE+ E P+L
Sbjct: 468 IGGHVSRYALDNIALEETHCRKTLCMYNDI*GCVQRTSYGLPCPCEIATKLLEEKPIL 295
Score = 48.1 bits (113), Expect(2) = 5e-17
Identities = 31/102 (30%), Positives = 46/102 (44%), Gaps = 1/102 (0%)
Frame = -2
Query: 520 IHPHWRKLTWDSSDRDMDPTLGLSFESEFDKLRTKFEESSHGVRMSIIESLRMITYPETT 579
I+ HW KL ++ G E E + ++ +++ + E LR +T+PETT
Sbjct: 286 IYHHWLKLRMGEESNEV----GFCVEVELKAIVEHLKKLPFQMKVEVKEGLRQLTFPETT 119
Query: 580 SMCAPTKKKTRGRPMGSTNKPKKFAKGCGE-TSTKRIPCRWE 620
M P +K P K A+ G+ TST RIP WE
Sbjct: 118 MMSPPPRKV----PTKGAKKKVDIARSKGKITSTSRIPSSWE 5
>TC82456 weakly similar to GP|18057159|gb|AAL58182.1 putative far-red
impaired response protein {Oryza sativa}, partial (7%)
Length = 1244
Score = 70.1 bits (170), Expect = 4e-12
Identities = 35/118 (29%), Positives = 60/118 (50%)
Frame = +2
Query: 208 VRSLFWAHPDSIHLFNEFPHVVILDSTYKTNRYRIPLLEMVGVTSTNLTYSIAFAYMQNE 267
+++LFWA + F V+ LD+TY T++Y +PL+ VGV T + A + N
Sbjct: 806 IQNLFWADARCRAAYEYFGEVITLDTTYLTSKYDLPLVPFVGVNHHGQTILLGCAILSNL 985
Query: 268 QKDEVVWAVNRLKDLIINEDNLPKVFVTDKDQVLMEALETVFPAARCLLCQFHVLKSV 325
+ W R + + + P +T++D+ + A+E FP AR C +H++K V
Sbjct: 986 DAKTLTWLFTRWLECM--HGHAPNGIITEEDKAMKNAIEVAFPKARHRWCLWHIMKKV 1153
>AW687028 weakly similar to GP|9909176|dbj| hypothetical protein~similar to
Arabidopsis thaliana F20D10.300, partial (11%)
Length = 662
Score = 65.9 bits (159), Expect = 7e-11
Identities = 46/154 (29%), Positives = 70/154 (44%), Gaps = 9/154 (5%)
Frame = +1
Query: 228 VVILDSTYKTNRYRIPLLEMVGVTSTNLTYSIAFAYMQNEQKDEVVWAVNRLKDLIINED 287
V+ D+TYK N+Y PL G + T A +++E + W +NR + + E+
Sbjct: 4 VLAFDTTYKKNKYNYPLCIFSGCNHHSQTIIFGVALLEDETIESYKWVLNRFLECM--EN 177
Query: 288 NLPKVFVTDKDQVLMEALETVFPAARCLLCQFHVLKSVTGEMK---------KLVKDIET 338
PK VTD D + EA++ VFP A LC +H+ K+ +K K + T
Sbjct: 178 KFPKAVVTDGDGSMREAIKQVFPDASHRLCAWHLHKNAQENIKKTPFLEGFRKAMYSNFT 357
Query: 339 REDITDRWNRLMYAANEVEYDKAVGAMSNTGELW 372
E D W+ L+ NE+E + V LW
Sbjct: 358 PEQFEDFWSELI-QKNELEGNAWVIKTYANKSLW 456
>BG449141 similar to GP|15983442|gb At1g10240/F14N23_12 {Arabidopsis
thaliana}, partial (33%)
Length = 690
Score = 48.5 bits (114), Expect = 1e-05
Identities = 31/157 (19%), Positives = 69/157 (43%), Gaps = 2/157 (1%)
Frame = +3
Query: 163 KQIYNEKQAYNRL--KRGSLTEMQHLMKLLEEHKYAHWSRVQDGTDVVRSLFWAHPDSIH 220
K + N Q++ +L + +L ++ + ++ + D + + ++ W++ SI
Sbjct: 180 KDVRNLLQSFRKLDPEEETLDLLRMCRNIKDKDPNFKFEYTLDANNRLENIAWSYASSIQ 359
Query: 221 LFNEFPHVVILDSTYKTNRYRIPLLEMVGVTSTNLTYSIAFAYMQNEQKDEVVWAVNRLK 280
L++ F V+ D+T++ + +PL VG+ + + +++E WA+
Sbjct: 360 LYDIFGDAVVFDTTHRLTAFDMPLGLWVGINNYGMPCFFGCVLLRDETVRSFSWAIKAF- 536
Query: 281 DLIINEDNLPKVFVTDKDQVLMEALETVFPAARCLLC 317
L P+ +TD++ L EAL P + C
Sbjct: 537 -LGFMNGKAPQTILTDQNICLKEALSAEMPMTKHAFC 644
>TC83834 similar to PIR|G96565|G96565 F6D8.26 [imported] - Arabidopsis
thaliana, partial (24%)
Length = 1032
Score = 44.3 bits (103), Expect = 2e-04
Identities = 50/261 (19%), Positives = 104/261 (39%), Gaps = 5/261 (1%)
Frame = +3
Query: 19 FESSKDLVNWAKAVGKEHGYVIIIQKSDY---GSDKRRAVMTLGCERGGKYKPSTSVLKR 75
FES D ++ KE G+ + ++ S + +K AV+ + + K ++ K
Sbjct: 231 FESYDDAYSYYICYAKEVGFCVRVKNSWFKRNSKEKYGAVLCCSSQGFKRTKDVNNLRKE 410
Query: 76 NRTGTKKCNCPFRLRARRSNKDKMWTVLVHSGIHNHDTAEVLQGHSYVGRLNPEEKAMVG 135
RTG CP +R + + W + + HNH + H + + + G
Sbjct: 411 TRTG-----CPAMIRMKLVESQR-WRICEVTLEHNHVLGAKI--HKSIKKNSLPSSDAEG 566
Query: 136 EMIEERVKASDILIAIRKHNPTNLTRIKQIYNEKQAYNRLKRGSLTEMQHLMKLLEEHKY 195
+ I +V + ++ N + R + +++ L++G + + + ++
Sbjct: 567 KTI--KVYHALVIDTEGNDNLNSNARDDRAFSKYSNKLNLRKGDTQAIYNFLCRMQLTN- 737
Query: 196 AHWSRVQDGTDV--VRSLFWAHPDSIHLFNEFPHVVILDSTYKTNRYRIPLLEMVGVTST 253
++ + D D +R+ W S F V+ D+TY N+Y IPL+ +VG+
Sbjct: 738 PNFFYLMDFNDEGHLRNALWVDAKSRAACGYFSDVIYFDNTYLVNKYEIPLVALVGINHH 917
Query: 254 NLTYSIAFAYMQNEQKDEVVW 274
+ + + E + W
Sbjct: 918 GQSVLLGCGLLAGEIIESYKW 980
>TC89654 weakly similar to PIR|T05644|T05644 hypothetical protein F20D10.290
- Arabidopsis thaliana, partial (65%)
Length = 1358
Score = 40.8 bits (94), Expect = 0.002
Identities = 21/64 (32%), Positives = 32/64 (49%), Gaps = 1/64 (1%)
Frame = +2
Query: 187 MKLLEEHKYAHWSRVQDGTDVVR-SLFWAHPDSIHLFNEFPHVVILDSTYKTNRYRIPLL 245
+K + +A + VQ D ++FWA ++ F VI D+TYKTN+YR+P
Sbjct: 143 LKRMRAENHAFFYAVQSDVDNAGGNIFWADETCRTNYSYFGDTVIFDTTYKTNQYRVPFA 322
Query: 246 EMVG 249
G
Sbjct: 323 SFTG 334
>TC90685 weakly similar to GP|11994595|dbj|BAB02650. receptor-like
serine/threonine kinase {Arabidopsis thaliana}, partial
(13%)
Length = 912
Score = 31.2 bits (69), Expect = 1.8
Identities = 14/27 (51%), Positives = 15/27 (54%)
Frame = -3
Query: 85 CPFRLRARRSNKDKMWTVLVHSGIHNH 111
C F L + NK KMW LVHS I H
Sbjct: 796 CSFHLPCKIRNKSKMWEKLVHSCIVQH 716
>TC90266 similar to SP|Q9C519|WRK6_ARATH WRKY transcription factor 6 (WRKY
DNA-binding protein 6) (AtWRKY6). [Mouse-ear cress],
partial (15%)
Length = 1376
Score = 30.8 bits (68), Expect = 2.4
Identities = 26/98 (26%), Positives = 40/98 (40%), Gaps = 6/98 (6%)
Frame = +2
Query: 20 ESSKDLVNWAKAVGKEHGYVIIIQKSDYGSDKRRAVMTLGCE------RGGKYKPSTSVL 73
E + + + + VG V I +SD+ A M GC+ + K P
Sbjct: 353 EINSKIEDQSSEVGCRRARVSIRARSDF------AFMVDGCQWRKYGQKTAKGNPCPRAY 514
Query: 74 KRNRTGTKKCNCPFRLRARRSNKDKMWTVLVHSGIHNH 111
R GT +CP R + +R KD+ + + G HNH
Sbjct: 515 YRCSMGT---SCPVRKQVQRCFKDESVFITTYEGNHNH 619
>TC76791 similar to GP|21311555|gb|AAM46778.1 15.8 kDa oleosin {Theobroma
cacao}, partial (64%)
Length = 933
Score = 30.8 bits (68), Expect = 2.4
Identities = 13/42 (30%), Positives = 24/42 (56%)
Frame = -1
Query: 167 NEKQAYNRLKRGSLTEMQHLMKLLEEHKYAHWSRVQDGTDVV 208
N+ AY + ++ + + KL E+H+ H +R+Q+ TD V
Sbjct: 792 NQHNAYTNKAKYLTSKTKEIQKLAEQHRTRHINRIQNKTDEV 667
>TC88799 similar to PIR|C71408|C71408 probable protein kinase - Arabidopsis
thaliana, partial (57%)
Length = 1648
Score = 29.6 bits (65), Expect = 5.4
Identities = 27/105 (25%), Positives = 41/105 (38%), Gaps = 10/105 (9%)
Frame = +2
Query: 596 STNKPKKFAKGCGETSTKRIPCRWETIDKEYPGSWRDSNTTPLQEPSPKVRSQK------ 649
+TN P A STK IP S+TTP PS SQK
Sbjct: 125 NTN*PASLAAAASPKSTKLIP----------------SSTTPTPSPSKSSTSQKTLTPPW 256
Query: 650 ----ASKASKASKASKVSTASKASKVQSPLIRMVCTKNKKPVSSY 690
+ K++ + ++ T+SK+ K + + + N PV S+
Sbjct: 257 SLALSVKSTPCAVSNTTLTSSKSMKSWLQKPKSILSLNSPPVVSF 391
>TC87453 similar to GP|22093671|dbj|BAC06965. similar to bHLH
(Basic-helix-loop-helix) transcription factor, partial
(26%)
Length = 1879
Score = 29.6 bits (65), Expect = 5.4
Identities = 13/35 (37%), Positives = 23/35 (65%)
Frame = +2
Query: 644 KVRSQKASKASKASKASKVSTASKASKVQSPLIRM 678
K RS+++S+AS ST + +SKVQ+P +++
Sbjct: 1280 KKRSEESSEASLKKPKQDTSTTNSSSKVQAPKVKL 1384
>TC83532 similar to PIR|T38639|T38639 proteasome component PUP2 homolog -
fission yeast (Schizosaccharomyces pombe), partial (97%)
Length = 901
Score = 29.6 bits (65), Expect = 5.4
Identities = 17/49 (34%), Positives = 25/49 (50%), Gaps = 1/49 (2%)
Frame = +1
Query: 630 WRDSNTTPLQEPS-PKVRSQKASKASKASKASKVSTASKASKVQSPLIR 677
W+D NTT PS PK S +++ SK S + + AS+ + S R
Sbjct: 82 WQDLNTTEESTPSPPKAVSSRSNTPSKPSSSDRRPLASQPRRASSSASR 228
>TC88624 similar to PIR|S51529|S51529 SPF1 protein - sweet potato, partial
(50%)
Length = 1496
Score = 29.6 bits (65), Expect = 5.4
Identities = 11/29 (37%), Positives = 15/29 (50%)
Frame = +2
Query: 84 NCPFRLRARRSNKDKMWTVLVHSGIHNHD 112
NCP R R++ D + + G HNHD
Sbjct: 1385 NCPVRKHVERASHDLRAVITTYEGKHNHD 1471
>TC77596 similar to GP|18377702|gb|AAL67001.1 putative clathrin binding
protein {Arabidopsis thaliana}, partial (44%)
Length = 2019
Score = 29.6 bits (65), Expect = 5.4
Identities = 21/55 (38%), Positives = 31/55 (56%), Gaps = 3/55 (5%)
Frame = +2
Query: 622 IDKEYPGSWRDSNTTPLQEPSPK---VRSQKASKASKASKASKVSTASKASKVQS 673
IDK+Y G R ++ +E S K VRS S+ +K+S+ASK S + S+ S
Sbjct: 743 IDKDYSGKSRHGVSSKNEENSFKKGSVRSVSKSQENKSSRASKSSANAVPSQSAS 907
>BG452313 homologue to PIR|T40261|T40 ubi4 protein - fission yeast
(Schizosaccharomyces pombe), partial (51%)
Length = 658
Score = 29.6 bits (65), Expect = 5.4
Identities = 13/27 (48%), Positives = 16/27 (59%)
Frame = +1
Query: 625 EYPGSWRDSNTTPLQEPSPKVRSQKAS 651
E P WR S TP SP+ R++KAS
Sbjct: 322 ERPSLWRSSRLTPSTMSSPRFRTRKAS 402
>TC77278 similar to GP|11493822|gb|AAG35658.1 transcription factor WRKY4
{Petroselinum crispum}, partial (40%)
Length = 1604
Score = 29.3 bits (64), Expect = 7.0
Identities = 13/42 (30%), Positives = 21/42 (49%)
Frame = +2
Query: 84 NCPFRLRARRSNKDKMWTVLVHSGIHNHDTAEVLQGHSYVGR 125
+CP + + +RS D+ V + G HNH ++ S GR
Sbjct: 779 SCPVKKKVQRSVDDQSMLVATYEGEHNHPQPPQIESTSGSGR 904
>TC93897 similar to PIR|D86320|D86320 hypothetical protein AAF27112.1
[imported] - Arabidopsis thaliana, partial (17%)
Length = 815
Score = 29.3 bits (64), Expect = 7.0
Identities = 22/82 (26%), Positives = 37/82 (44%)
Frame = +3
Query: 581 MCAPTKKKTRGRPMGSTNKPKKFAKGCGETSTKRIPCRWETIDKEYPGSWRDSNTTPLQE 640
+C P K++T +P+G + P E T R+ D+ ++ NT + E
Sbjct: 144 VCGPEKRRTSRKPVGLSKLP------LAEDLTSRVQTSLNMDDRSV-NVLKEENTN-IGE 299
Query: 641 PSPKVRSQKASKASKASKASKV 662
+PK + K+ AS AS+V
Sbjct: 300 MAPKPTAGKSEDASHKKDASQV 365
>TC89563 similar to GP|15865943|gb|AAL10121.1 NADH dehydrogenase subunit 2
{Varanus salvator cumingi}, partial (6%)
Length = 1400
Score = 29.3 bits (64), Expect = 7.0
Identities = 25/91 (27%), Positives = 40/91 (43%), Gaps = 1/91 (1%)
Frame = -3
Query: 593 PMGSTNKPKKFAKGCGETSTKRIPCRWETIDKEYPGSWRDSNTTPLQE-PSPKVRSQKAS 651
P +T P + A E S K + KE +++ T P +E P PK SQK +
Sbjct: 1155 PKETTTNPPEEAPRPKEPSQKVNEQKGTPKAKEEKVETKETTTNPPEEAPRPKEPSQKGT 976
Query: 652 KASKASKASKVSTASKASKVQSPLIRMVCTK 682
+K KA T + + ++P + + TK
Sbjct: 975 PKAKEEKAETNETTTNPPE-EAPKTKGITTK 886
>TC77547 similar to PIR|T48354|T48354 hypothetical protein F12E4.60 -
Arabidopsis thaliana, partial (53%)
Length = 2073
Score = 29.3 bits (64), Expect = 7.0
Identities = 13/47 (27%), Positives = 27/47 (56%), Gaps = 2/47 (4%)
Frame = +3
Query: 709 DFIN--VKGDGHCGYRCVASLKGLAEDDWPLIRRELLTEIDSDANMY 753
DF+ V+GDG+C +R ++ D +RR+++ ++ S ++Y
Sbjct: 1221 DFVEHKVQGDGNCQFRALSDQLYNTPDHHKFVRRKVVNQLKSHPDIY 1361
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.134 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,778,847
Number of Sequences: 36976
Number of extensions: 414693
Number of successful extensions: 2103
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 2075
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2099
length of query: 898
length of database: 9,014,727
effective HSP length: 105
effective length of query: 793
effective length of database: 5,132,247
effective search space: 4069871871
effective search space used: 4069871871
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 63 (28.9 bits)
Lotus: description of TM0546.8