BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0544.8
(1753 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG584039 homologue to GP|4249411|gb|A unknown protein {Arabidops... 199 6e-51
BG448680 homologue to GP|4249411|gb|A unknown protein {Arabidops... 199 1e-50
TC92238 weakly similar to GP|14456138|emb|CAC41653. hypothetical... 37 0.088
TC84295 similar to PIR|T45887|T45887 hypothetical protein F4P12.... 35 0.33
AL381417 33 1.3
CA917534 32 1.7
TC76693 adenosylhomocysteinase [Medicago truncatula] 32 1.7
TC88039 homologue to GP|15623873|dbj|BAB67931. hypothetical prot... 32 2.8
BQ137858 31 4.8
TC79156 weakly similar to GP|11034603|dbj|BAB17127. putative rec... 31 4.8
TC77188 similar to PIR|T04862|T04862 probable serine/threonine-s... 31 4.8
TC86137 weakly similar to PIR|A96673|A96673 probable cytochrome ... 31 4.8
BQ752248 31 4.8
CB894695 similar to GP|22136262|gb| unknown protein {Arabidopsis... 31 4.8
AL375169 GP|4959710|gb| Skp1 {Medicago sativa}, partial (18%) 31 4.8
TC90846 similar to GP|20260286|gb|AAM13041.1 unknown protein {Ar... 30 6.3
BQ148918 weakly similar to PIR|T05232|T05 hypothetical protein F... 30 8.2
BG646347 similar to PIR|T05135|T05 hypothetical protein F7H19.22... 30 8.2
TC89841 similar to PIR|T02307|T02307 probable membrane transport... 30 8.2
TC87223 similar to GP|21536674|gb|AAM61006.1 unknown {Arabidopsi... 30 8.2
>BG584039 homologue to GP|4249411|gb|A unknown protein {Arabidopsis thaliana},
partial (23%)
Length = 800
Score = 199 bits (507), Expect = 6e-51
Identities = 99/124 (79%), Positives = 112/124 (89%), Gaps = 2/124 (1%)
Frame = +3
Query: 1632 SFYDLDISD-AHGCGKFPGPMAIIVSEETP-QGIIGDTLSKFSIWGLYITFVLAVGRFIR 1689
+F D++ S+ + CG GPMAII+SEETP QGI+GDTLSKFSIWGLYITFVLAVGRFIR
Sbjct: 27 AFKDINPSNLSRFCGGLTGPMAIIISEETPPQGILGDTLSKFSIWGLYITFVLAVGRFIR 206
Query: 1690 LQCSDLRMRIPFENLPSCDRLMALCEDIYAARAEGELEVEEVLYWTLVKIYRTPHMLLEY 1749
LQCSDLRMRIP+ENLPSCDRL+A+CEDIY ARAEGEL VEEVLYWTLVKIYR+PHMLLEY
Sbjct: 207 LQCSDLRMRIPYENLPSCDRLIAICEDIYNARAEGELGVEEVLYWTLVKIYRSPHMLLEY 386
Query: 1750 TQAE 1753
T+ +
Sbjct: 387 TKPD 398
>BG448680 homologue to GP|4249411|gb|A unknown protein {Arabidopsis thaliana},
partial (29%)
Length = 659
Score = 199 bits (505), Expect = 1e-50
Identities = 96/111 (86%), Positives = 104/111 (93%), Gaps = 1/111 (0%)
Frame = +1
Query: 1644 CGKFPGPMAIIVSEETP-QGIIGDTLSKFSIWGLYITFVLAVGRFIRLQCSDLRMRIPFE 1702
CG GPMAII+SEETP QGI+GDTLSKFSIWGLYITFVLAVGRFIRLQCSDLRMRIP+E
Sbjct: 22 CGGLTGPMAIIISEETPPQGILGDTLSKFSIWGLYITFVLAVGRFIRLQCSDLRMRIPYE 201
Query: 1703 NLPSCDRLMALCEDIYAARAEGELEVEEVLYWTLVKIYRTPHMLLEYTQAE 1753
NLPSCDRL+A+CEDIY ARAEGEL VEEVLYWTLVKIYR+PHMLLEYT+ +
Sbjct: 202 NLPSCDRLIAICEDIYNARAEGELGVEEVLYWTLVKIYRSPHMLLEYTKPD 354
>TC92238 weakly similar to GP|14456138|emb|CAC41653. hypothetical protein
{Ustilago maydis}, partial (5%)
Length = 641
Score = 36.6 bits (83), Expect = 0.088
Identities = 20/54 (37%), Positives = 29/54 (53%), Gaps = 9/54 (16%)
Frame = -3
Query: 837 GQEPTDDS--------SEDEEYYEIENQNSGAEPLEPTFSTNSIN-EHTGSDTS 881
GQ+P D+S EDEEYY+ ++S E E F N +N ++ G DT+
Sbjct: 504 GQQPNDESIFLQNGKDDEDEEYYDDHEEDSDEEFFEEYFDDNELNPQNNGKDTN 343
>TC84295 similar to PIR|T45887|T45887 hypothetical protein F4P12.170 -
Arabidopsis thaliana, partial (64%)
Length = 656
Score = 34.7 bits (78), Expect = 0.33
Identities = 19/49 (38%), Positives = 26/49 (52%)
Frame = +3
Query: 302 LKWNRKRLLFLRKERLEMQKTALKVSLKFWIENMFTLFGLEINMIALLL 350
+ WN +R F E ++ K + +LKFW FTL G I +I LLL
Sbjct: 357 VSWNPRRSDFPLSEEVDPIKRTERSNLKFWTSPTFTLGGALIILIVLLL 503
>AL381417
Length = 509
Score = 32.7 bits (73), Expect = 1.3
Identities = 20/72 (27%), Positives = 36/72 (49%)
Frame = +2
Query: 88 RLLVRHTEKILFLALFYASLSPISAFGFLYLLGLINCSRLPKSSQIPAKVFLVYSGLLIM 147
+ L H +ILF A FY+ +SP+S+ + +++ + P + IPA + G
Sbjct: 92 KALFPHVPRILFSAAFYSRISPLSSVPVPP*IAILS-NT*PHQNSIPAVTSVSLXGSNAS 268
Query: 148 VEYLFQMLGDQA 159
+Y +Q+ QA
Sbjct: 269 SQYYYQVKSSQA 304
>CA917534
Length = 616
Score = 32.3 bits (72), Expect = 1.7
Identities = 21/46 (45%), Positives = 27/46 (58%), Gaps = 1/46 (2%)
Frame = +1
Query: 790 RNRY-KLPGKRNALVSAVHFIGSGISQAQSLGNMAVNNLVNYLKIE 834
+N+Y K RNAL AV F+ I++ QSL N NN V +KIE
Sbjct: 100 KNKYLKAQESRNALRQAVKFLELKINEIQSLNNHNNNNKVCDVKIE 237
>TC76693 adenosylhomocysteinase [Medicago truncatula]
Length = 2035
Score = 32.3 bits (72), Expect = 1.7
Identities = 24/96 (25%), Positives = 43/96 (44%)
Frame = +1
Query: 1598 YFRVTGSGDVRSLEQSVELVSGDLVLNRGNPEWWSFYDLDISDAHGCGKFPGPMAIIVSE 1657
+ TG+ D+ + ++ + +V N G+ +D +I D HG +PG I +
Sbjct: 1126 FVTTTGNKDIIMVSDMKKMKNNAIVCNIGH------FDNEI-DMHGLETYPGVKRITIKP 1284
Query: 1658 ETPQGIIGDTLSKFSIWGLYITFVLAVGRFIRLQCS 1693
+T + + +T S VLA GR + L C+
Sbjct: 1285 QTDRWVFPETKSGI--------IVLAEGRLMNLGCA 1368
>TC88039 homologue to GP|15623873|dbj|BAB67931. hypothetical protein {Oryza
sativa (japonica cultivar-group)}, partial (12%)
Length = 903
Score = 31.6 bits (70), Expect = 2.8
Identities = 16/56 (28%), Positives = 31/56 (54%)
Frame = +2
Query: 909 ILIYLWNFSLLSVVYLAALFLYALCQNTGPSYIFWVLILIYTEVCILLQYLYQIII 964
+++ LW + S+V + L++ CQN +F VL+L+ +L++ LY + I
Sbjct: 221 MVVVLWYMIMNSLVVIVQTLLFSTCQNVSTISVFLVLMLL----MLLMRNLYLVNI 376
>BQ137858
Length = 967
Score = 30.8 bits (68), Expect = 4.8
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = +1
Query: 1263 FFLIVLDRIIYLCSFATAKVIFYLFSLVLFTYSV 1296
F+ + D I+Y+ F ++ YLFSL+L YS+
Sbjct: 112 FYTLPDDLILYVLDFFLILIVLYLFSLLLLPYSL 213
>TC79156 weakly similar to GP|11034603|dbj|BAB17127. putative receptor kinase
{Oryza sativa (japonica cultivar-group)}, partial (18%)
Length = 1246
Score = 30.8 bits (68), Expect = 4.8
Identities = 20/61 (32%), Positives = 32/61 (51%), Gaps = 1/61 (1%)
Frame = -3
Query: 1344 YRQFLTSSVSRINVMGFRLYRAIPFLYEL-RCVLDWSCTTTSLTMYDWLKLEDIHASLFL 1402
Y ++SS S +N+ GF L + L + + CTTTS+ + + +LEDI S +
Sbjct: 527 YFSVISSSFSGVNLTGFSLLMLLNILSMVANSCFESVCTTTSMLLVEENELEDI*LSFSV 348
Query: 1403 V 1403
V
Sbjct: 347 V 345
>TC77188 similar to PIR|T04862|T04862 probable serine/threonine-specific
protein kinase (EC 2.7.1.-) F28A21.110 - Arabidopsis
thaliana, partial (79%)
Length = 1881
Score = 30.8 bits (68), Expect = 4.8
Identities = 29/123 (23%), Positives = 50/123 (40%), Gaps = 1/123 (0%)
Frame = -1
Query: 987 TSSFVTSNLPFFLIYIFTLLQISITMKGGGWTISVDPSFFRRRNQKSYLEDVKCSTYHER 1046
T S + N PF L+Y+ +LQ+S S + E+ +C
Sbjct: 1701 TQSIINKNYPFILLYV--ILQVS----------KFRDSILSECLSMAESENARCCKDSSG 1558
Query: 1047 VQRLFLPLSNAL-KLLTRSLCRYWKSLTWGAETPPYFVQLSMEVNLWPKEGIQPKRIESK 1105
V L P ++L KL +K+L++ +PP+F + KEG+ K +
Sbjct: 1557 VV*LEEPADSSLVKLCKAGFNSVFKNLSYSTLSPPFFF-----TSTTTKEGVNSKTSAAI 1393
Query: 1106 INK 1108
+N+
Sbjct: 1392 VNR 1384
>TC86137 weakly similar to PIR|A96673|A96673 probable cytochrome P450
F13O11.25 [imported] - Arabidopsis thaliana, partial
(69%)
Length = 1695
Score = 30.8 bits (68), Expect = 4.8
Identities = 35/135 (25%), Positives = 58/135 (42%), Gaps = 2/135 (1%)
Frame = -3
Query: 965 QHTEFEFPMSLLQELGFPAKKITSSFVTSNLPFFLIYIFTLLQIS--ITMKGGGWTISVD 1022
Q F +S G K S++ T+ ++ +F LL ++ + + G T S
Sbjct: 964 QRLTISFSLSFRFSSGHSNSKRVSAYETT-----VLILFNLLSLTCFLALISGSRTSS*S 800
Query: 1023 PSFFRRRNQKSYLEDVKCSTYHERVQRLFLPLSNALKLLTRSLCRYWKSLTWGAETPPYF 1082
S F+ N +L + T+ ++ L LP N + L TRS+ + SLT P
Sbjct: 799 LSNFK--NSSHWLRNKILVTFGQKFSLLNLPKFNIIFLCTRSISLIFSSLT----LSPKH 638
Query: 1083 VQLSMEVNLWPKEGI 1097
++ S E + K GI
Sbjct: 637 IKTSKENIAYLKCGI 593
>BQ752248
Length = 882
Score = 30.8 bits (68), Expect = 4.8
Identities = 14/48 (29%), Positives = 25/48 (51%)
Frame = +1
Query: 683 EKMKSEMLNLQNQLHSMSTDANYSNASLKNDGLRERRNSFLENEFRKQ 730
E S +NLQ Q+ S+ A + N + + +R+R +EN++ Q
Sbjct: 508 EDESSRAVNLQRQISSLMEGAEHRNHEEQEEEIRKREEKEIENDYNGQ 651
>CB894695 similar to GP|22136262|gb| unknown protein {Arabidopsis thaliana},
partial (13%)
Length = 794
Score = 30.8 bits (68), Expect = 4.8
Identities = 40/179 (22%), Positives = 80/179 (44%), Gaps = 7/179 (3%)
Frame = +3
Query: 556 IFRYLRMYNFAVIVLSLAYQSPFLGDFSEIKSGFIEYINELVGFQ--KYDYGFRIT---- 609
+ + +YNF VL L+ + L +F F+E +NE++ + + G RI+
Sbjct: 150 LLTFCLIYNFFCSVLFLSSKILSL*EFPFSIFNFLEILNEIMALELCSQNCGPRISFSHD 329
Query: 610 -SRSALVEIIIYMLVSLQSYMFSFPEFDYVSTYLEKEQIGAILRQQEKKAAWKTAQLRHI 668
S+S + + + + S S + S +FD+ + L +E +A + I
Sbjct: 330 FSQSDFIPVEQHPITSKSSGLNSSIDFDF--------NVSESLNLEESSSAAELFSDGRI 485
Query: 669 RKAEELKHLRSLQVEKMKSEMLNLQNQLHSMSTDANYSNASLKNDGLRERRNSFLENEF 727
AE ++ QV +S L Q+ + S + +YS + +++G R+ S E+++
Sbjct: 486 LPAE----IKKKQVPLKQS--LTTQSPSPNPSLNPSYSTCNNESNGKNLRKESTKESKY 644
>AL375169 GP|4959710|gb| Skp1 {Medicago sativa}, partial (18%)
Length = 466
Score = 30.8 bits (68), Expect = 4.8
Identities = 12/30 (40%), Positives = 20/30 (66%)
Frame = +3
Query: 904 CYCCFILIYLWNFSLLSVVYLAALFLYALC 933
CY C +L+YL ++ +LSV Y++ +Y C
Sbjct: 303 CYIC*VLVYLLDYFVLSVDYVSLDSVYLYC 392
>TC90846 similar to GP|20260286|gb|AAM13041.1 unknown protein {Arabidopsis
thaliana}, partial (61%)
Length = 813
Score = 30.4 bits (67), Expect = 6.3
Identities = 16/42 (38%), Positives = 24/42 (57%)
Frame = +3
Query: 407 MNLEIEEHVPCRDCWRVSDIYFSYCRKCWLGIIVDDPRMLIG 448
+NLE EH + +DI+ + RKCW ++V DPR + G
Sbjct: 477 LNLEEMEHGMMWLPFYHTDIWRAVIRKCWCVLLVLDPRKMSG 602
>BQ148918 weakly similar to PIR|T05232|T05 hypothetical protein F18A5.20 -
Arabidopsis thaliana, partial (17%)
Length = 639
Score = 30.0 bits (66), Expect = 8.2
Identities = 43/152 (28%), Positives = 59/152 (38%), Gaps = 6/152 (3%)
Frame = +1
Query: 637 YVSTYLEKEQIGAILRQQEKKAAWKTAQLRHIRKAEELKHLRSLQVEKMKSEMLNLQNQL 696
Y + YLE E+ E+ AA A E + + LQ EK EM Q +
Sbjct: 202 YAALYLELEK--------ERAAAATAAD-------EAMAMISRLQEEKSSMEMEMRQYER 336
Query: 697 ---HSMSTDANYSNASLKNDGLRERRNSFLENE---FRKQALDTNTESIGTLDVNQSLLS 750
+S D N + RER N FLE E FR+ +L + E + + L
Sbjct: 337 LIEERVSYDEEEMNIMQEILIRRERENLFLEKELETFRQMSLTESCE-LHSKSAEVRLNE 513
Query: 751 EKSESPLVPEYMKHPMGSPHGIVEVKERTKNN 782
K SPL E P ++ VK+ NN
Sbjct: 514 WKRSSPLSFETCDDPSQIESTVLIVKQDCTNN 609
>BG646347 similar to PIR|T05135|T05 hypothetical protein F7H19.220 -
Arabidopsis thaliana, partial (33%)
Length = 752
Score = 30.0 bits (66), Expect = 8.2
Identities = 21/75 (28%), Positives = 37/75 (49%), Gaps = 1/75 (1%)
Frame = -2
Query: 302 LKWNRKRLLFLRKERLEMQKTALKVSLKFW-IENMFTLFGLEINMIALLLASFAVLNAIS 360
L++ R+ + + K EM+ +LKV +++W E ++ FG I L+ F L +S
Sbjct: 274 LQYRRRHVRIIGKAIFEMEFISLKVCVRWW*NEKVWFFFGFGIK--GLVRIMFD*LGFVS 101
Query: 361 LLYIASLAACVLLHR 375
L+ C+ L R
Sbjct: 100 LMVFGFFFLCLSLGR 56
>TC89841 similar to PIR|T02307|T02307 probable membrane transporter
At2g34190 [imported] - Arabidopsis thaliana, partial
(63%)
Length = 1165
Score = 30.0 bits (66), Expect = 8.2
Identities = 22/84 (26%), Positives = 37/84 (43%)
Frame = +2
Query: 889 VRHIWSRMRSNNEVVCYCCFILIYLWNFSLLSVVYLAALFLYALCQNTGPSYIFWVLILI 948
VR IWS + N + +CC+IL ++W+ S + A ++ N P +
Sbjct: 545 VR*IWSFICINT-IPYFCCYILCFVWSCSFCRAIIFA---IHQHEFNEEP---------L 685
Query: 949 YTEVCILLQYLYQIIIQHTEFEFP 972
Y C L+ +Y I Q ++ P
Sbjct: 686 YHWCCPFLRLVYS*IFQRIHYQGP 757
>TC87223 similar to GP|21536674|gb|AAM61006.1 unknown {Arabidopsis
thaliana}, partial (69%)
Length = 1917
Score = 30.0 bits (66), Expect = 8.2
Identities = 24/112 (21%), Positives = 50/112 (44%), Gaps = 13/112 (11%)
Frame = +2
Query: 344 NMIALLLASFAVLNAISLLYIASLAACVLLHRLLIKKLWPIFVFLFASV---VTVEYL-- 398
NM+ ++L F + ++ + L C++ + + L P F+ A + + +E+
Sbjct: 383 NMLLIVLLIFHLFLMMNCFHSNGLGRCLIASSFVKRSLKPFFILTKARL*DHLWIEWFRS 562
Query: 399 ------AIWMRLTFMNLEIEEHVPCRDCWRVSDIYFSYCRKCWL--GIIVDD 442
+WM +T + V R+CWR ++F + W+ G++V D
Sbjct: 563 ILKGV*RLWMSVTPFVTALNRFVFGRNCWR---LFFMH----WIIRGVLVKD 697
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.325 0.139 0.421
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 59,201,245
Number of Sequences: 36976
Number of extensions: 942044
Number of successful extensions: 7411
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 5474
Number of HSP's successfully gapped in prelim test: 287
Number of HSP's that attempted gapping in prelim test: 1714
Number of HSP's gapped (non-prelim): 6084
length of query: 1753
length of database: 9,014,727
effective HSP length: 110
effective length of query: 1643
effective length of database: 4,947,367
effective search space: 8128523981
effective search space used: 8128523981
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 65 (29.6 bits)
Lotus: description of TM0544.8