BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0544.13
(545 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC86761 homoglutathione synthetase GSHS2 [Medicago truncatula] 834 0.0
TC88431 glutathione synthetase GSHS1 [Medicago truncatula] 761 0.0
TC87577 weakly similar to PIR|H96604|H96604 probable 3'-5' exonu... 30 1.8
TC78586 similar to PIR|T01041|T01041 hypothetical protein YUP8H1... 30 2.4
BQ151241 GP|19917424|gb conserved hypothetical protein {Methanos... 30 2.4
TC91390 similar to GP|15293203|gb|AAK93712.1 unknown protein {Ar... 30 3.1
TC78495 homologue to PIR|S53504|S53504 extensin-like protein S3 ... 30 3.1
TC76771 homologue to SP|Q42961|PGKH_TOBAC Phosphoglycerate kinas... 30 3.1
TC90143 weakly similar to GP|9280639|gb|AAF86508.1| F21B7.1 {Ara... 29 4.0
TC77043 similar to PIR|S33618|S33618 glutathione peroxidase (EC ... 29 4.0
CB893818 homologue to GP|4582783|emb starch synthase isoform V ... 29 4.0
AL370212 29 4.0
TC92128 similar to PIR|B86389|B86389 hypothetical protein AAG292... 29 4.0
BF645392 weakly similar to GP|7630034|em putative protein {Arabi... 29 5.3
TC77344 similar to GP|4210332|emb|CAA11553.1 2-oxoglutarate dehy... 29 5.3
TC90647 similar to GP|14517552|gb|AAK62666.1 F17J6.12/F17J6.12 {... 28 6.9
CB894765 28 6.9
CA922196 similar to GP|2895758|gb|A phosphatidic acid-preferring... 28 9.0
BI269959 similar to GP|20805873|gb| long chain acyl-CoA syntheta... 28 9.0
>TC86761 homoglutathione synthetase GSHS2 [Medicago truncatula]
Length = 2017
Score = 834 bits (2154), Expect = 0.0
Identities = 424/546 (77%), Positives = 479/546 (87%), Gaps = 7/546 (1%)
Frame = +3
Query: 7 GSGCCFITSGTSHSFSHSTFPSFSSIQLHHQSLSLSLSFPKHHLKLIMSQHLSVSSSPIV 66
GSGC + + S+F SS HH+S+ LSL FPK+ +LS +++ I
Sbjct: 78 GSGCLVWFPHSCTNIFPSSF--VSSTSQHHRSV-LSLFFPKNRNMSQQQLNLSSTTTNIE 248
Query: 67 EEVNDSS------SF-DYHQIDPELLENVVYDALVYATLNGLLVGDKSVQRSGKVPGVGL 119
EV D+S SF DYHQID ++LEN+VYDALV+ATLNGLLVGDKSV+RSG+VPGVGL
Sbjct: 249 VEVVDNSPPMTLPSFNDYHQIDDKVLENIVYDALVFATLNGLLVGDKSVERSGRVPGVGL 428
Query: 120 VHLPFSLLPPPLPESYWKQACELAPLFNELVDRVSLDGKFLQESLSRTKKADEFTSRLLD 179
VHLPFSLLPPPLPE++WKQAC+L+PLFNELVDRVSLDGKFLQ+SLS+TKKADEFTSRLLD
Sbjct: 429 VHLPFSLLPPPLPETHWKQACDLSPLFNELVDRVSLDGKFLQQSLSKTKKADEFTSRLLD 608
Query: 180 IHSKMLEINKKEEIRMGLHRSDYMLDEKTKRLLQIEMNTISTSFSGVGCVTTELHRNILS 239
IH+KMLE+NKKEEIRMGL RSDYMLDEKTK LLQIEMNTISTSFSGVG V TELHRNILS
Sbjct: 609 IHAKMLEMNKKEEIRMGLFRSDYMLDEKTKSLLQIEMNTISTSFSGVGVVMTELHRNILS 788
Query: 240 HYGKLLGLDSIRVPANSATNKFAETLAKAWLEYNNPRAVIMIMVQPEEHNMYDQHMISAV 299
HYGKLLGLDS RVP N+A +++AE LAKAW EYNNPRAVI+++VQ EE NMYDQH +SA+
Sbjct: 789 HYGKLLGLDSERVPVNTADSRYAEALAKAWKEYNNPRAVIIVVVQGEERNMYDQHFVSAI 968
Query: 300 LRDTYHIPTIRKTLAEVDQEGAVLPDGTLSVDGQAIAIVYFRAGYTPKDYPSESEWRARL 359
LR+ +HI TIRKTLAE++QEG +LPDGTLSVDGQA+AIVYFRAGYTP DYPSESEWRARL
Sbjct: 969 LREKHHITTIRKTLAEINQEGEILPDGTLSVDGQAVAIVYFRAGYTPVDYPSESEWRARL 1148
Query: 360 LMEQSSAIKCPPISYHLVGTKKIQQELAKPNVLERFFENKDDVAKLRECFAGLWSLDDSD 419
L+EQSSAIKCP ISYHLVGTKKIQQELA+PNVLERFFENK+D+AKLR+CFAGLWSL+DSD
Sbjct: 1149LIEQSSAIKCPNISYHLVGTKKIQQELARPNVLERFFENKEDIAKLRKCFAGLWSLEDSD 1328
Query: 420 IVKQAIEKPELFVMKPQREGGGNNIYGDDLRETLIKLQKSGSEEDAAYILMQRIFPTSTA 479
IV +AI KPELFVMKPQREGGGNNIYGDDLRETL +LQK GS+EDAAYILMQRIFP +TA
Sbjct: 1329IVNEAIAKPELFVMKPQREGGGNNIYGDDLRETLKRLQKPGSQEDAAYILMQRIFPANTA 1508
Query: 480 AILMRNGCWHKDHVLTEFGIFGTYLRNKDKVIINSESGYMVRTKMSSSDEGGVLPGFGVI 539
+ILMRNG HKDHV++EFGIF TYLRNKDKVI+N+ESGYMVRTK S SDEGGVLPGFGVI
Sbjct: 1509SILMRNGFLHKDHVISEFGIFSTYLRNKDKVIVNNESGYMVRTKPSLSDEGGVLPGFGVI 1688
Query: 540 DSVYLT 545
DSVYLT
Sbjct: 1689DSVYLT 1706
>TC88431 glutathione synthetase GSHS1 [Medicago truncatula]
Length = 1952
Score = 761 bits (1965), Expect = 0.0
Identities = 380/524 (72%), Positives = 445/524 (84%), Gaps = 2/524 (0%)
Frame = +2
Query: 24 STFPSFSSIQLHHQSLSLSLSFPKHHLKLIMSQHLSVSSSPIVEEVNDSSSF--DYHQID 81
S FPS SS S S S++F L + +HL+++ + S F DYH ID
Sbjct: 116 SFFPSSSSSS--SSSSSSSINFSTKPLPMSHHKHLTINGDE-QNIIKPSPLFVDDYHNID 286
Query: 82 PELLENVVYDALVYATLNGLLVGDKSVQRSGKVPGVGLVHLPFSLLPPPLPESYWKQACE 141
+LL+N+ YDALV+A+L+GLL+GDKS Q+SG VPGVGL+H PFSL P PES WKQAC+
Sbjct: 287 QQLLDNIAYDALVWASLHGLLMGDKSYQKSGMVPGVGLMHAPFSLFPTSFPESKWKQACD 466
Query: 142 LAPLFNELVDRVSLDGKFLQESLSRTKKADEFTSRLLDIHSKMLEINKKEEIRMGLHRSD 201
LAP+FNELVDRVSLDGKFLQESLSRTK+ DEFTSRLLDIHSKML++NKKEEIR GLHRSD
Sbjct: 467 LAPIFNELVDRVSLDGKFLQESLSRTKQVDEFTSRLLDIHSKMLQLNKKEEIRFGLHRSD 646
Query: 202 YMLDEKTKRLLQIEMNTISTSFSGVGCVTTELHRNILSHYGKLLGLDSIRVPANSATNKF 261
YMLDE+TK LLQIE+NTIS+SF+G + T+LHR IL+ +GKLLGLDS +VPAN+A ++
Sbjct: 647 YMLDEQTKSLLQIELNTISSSFAGFSTLVTQLHRYILTCHGKLLGLDSEKVPANNAVSQN 826
Query: 262 AETLAKAWLEYNNPRAVIMIMVQPEEHNMYDQHMISAVLRDTYHIPTIRKTLAEVDQEGA 321
AE LAKAW EYNNPRAVIM +VQ EE NMYDQH +SAVLRD + I +RKT+AEVDQEG
Sbjct: 827 AEALAKAWSEYNNPRAVIMTVVQAEERNMYDQHFLSAVLRDKHGITIVRKTMAEVDQEGE 1006
Query: 322 VLPDGTLSVDGQAIAIVYFRAGYTPKDYPSESEWRARLLMEQSSAIKCPPISYHLVGTKK 381
+LPDGTLSV+GQA+A++YFRAGYTP DYPSESEWRARLLMEQSSA+KCP ISYHLVGTKK
Sbjct: 1007ILPDGTLSVNGQAVAVIYFRAGYTPVDYPSESEWRARLLMEQSSAVKCPSISYHLVGTKK 1186
Query: 382 IQQELAKPNVLERFFENKDDVAKLRECFAGLWSLDDSDIVKQAIEKPELFVMKPQREGGG 441
IQQELAKP VLERF ENKDD+AK+RECFAGLWSLDDSDIVK+AIE+PELFVMKPQREGGG
Sbjct: 1187IQQELAKPGVLERFLENKDDIAKMRECFAGLWSLDDSDIVKKAIERPELFVMKPQREGGG 1366
Query: 442 NNIYGDDLRETLIKLQKSGSEEDAAYILMQRIFPTSTAAILMRNGCWHKDHVLTEFGIFG 501
NNIYGD +R+TLIKLQK+GS+EDAAYILMQRIFP +AA+LMRNG HKDH ++E GIFG
Sbjct: 1367NNIYGDAVRDTLIKLQKTGSQEDAAYILMQRIFPNISAAVLMRNGGCHKDHAISELGIFG 1546
Query: 502 TYLRNKDKVIINSESGYMVRTKMSSSDEGGVLPGFGVIDSVYLT 545
TYLRNKD+V++N++SGY++RTK+SSSDEGGV GF VIDSVYLT
Sbjct: 1547TYLRNKDRVVMNNQSGYLMRTKISSSDEGGVAAGFAVIDSVYLT 1678
>TC87577 weakly similar to PIR|H96604|H96604 probable 3'-5' exonuclease
[imported] - Arabidopsis thaliana, partial (54%)
Length = 1980
Score = 30.4 bits (67), Expect = 1.8
Identities = 26/114 (22%), Positives = 54/114 (46%)
Frame = +1
Query: 157 GKFLQESLSRTKKADEFTSRLLDIHSKMLEINKKEEIRMGLHRSDYMLDEKTKRLLQIEM 216
G+ + + K + F +L ++ + + +K+ + G ++ ++++ T L++ ++
Sbjct: 499 GQIKHDEMIVMKILETFDVKLPNVEKALCQNKEKDYLSCG--KAQELIEQYTFELVKSQL 672
Query: 217 NTISTSFSGVGCVTTELHRNILSHYGKLLGLDSIRVPANSATNKFAETLAKAWL 270
TI+ VT H +I SHYG+ LD I+ A K+A + K L
Sbjct: 673 YTIA--------VTLMEHFSI-SHYGQSFLLDMIKSNQFKAAEKWATFMGKPML 807
>TC78586 similar to PIR|T01041|T01041 hypothetical protein YUP8H12R.24 -
Arabidopsis thaliana, partial (38%)
Length = 2831
Score = 30.0 bits (66), Expect = 2.4
Identities = 23/93 (24%), Positives = 44/93 (46%), Gaps = 2/93 (2%)
Frame = +1
Query: 133 ESYWKQACELAPLFNELVDRVSLDGKFLQE--SLSRTKKADEFTSRLLDIHSKMLEINKK 190
E++ + C+LA L N L+ + KFL++ LS+ K LL + + +I
Sbjct: 616 ENFESKKCKLAELGNALITDPESNIKFLKDMVQLSKDKDLTIVKLGLLSLLAVFRDIIPG 795
Query: 191 EEIRMGLHRSDYMLDEKTKRLLQIEMNTISTSF 223
IR+ + M KT R ++ +T+ +++
Sbjct: 796 YRIRLATEKEQEMKVSKTVRKMRFYESTLLSAY 894
>BQ151241 GP|19917424|gb conserved hypothetical protein {Methanosarcina
acetivorans str. C2A}, partial (3%)
Length = 560
Score = 30.0 bits (66), Expect = 2.4
Identities = 19/46 (41%), Positives = 25/46 (54%)
Frame = +1
Query: 20 SFSHSTFPSFSSIQLHHQSLSLSLSFPKHHLKLIMSQHLSVSSSPI 65
S S FPS S+I +H SL SLS P+ L + H + SS P+
Sbjct: 271 SSGFSLFPSLSAISIHSPSLFTSLSSPR--LLCRGASHSTHSSPPV 402
>TC91390 similar to GP|15293203|gb|AAK93712.1 unknown protein {Arabidopsis
thaliana}, partial (28%)
Length = 657
Score = 29.6 bits (65), Expect = 3.1
Identities = 31/105 (29%), Positives = 49/105 (46%), Gaps = 2/105 (1%)
Frame = +2
Query: 27 PSFSSIQLHHQSLSLSLSFPKHHLKLIMSQHLSVSSSPIVEEVNDSSSFDYHQIDPELLE 86
PS + Q H LSFPK H KL +S S SSS + + +++ +Q E++E
Sbjct: 158 PSLTHFQSHPP-----LSFPKLHPKLSISTVASASSS----KNSSNNTNPNNQSKEEIIE 310
Query: 87 NVVYDALVYATLNGLLVGDKSVQRSGKVPG--VGLVHLPFSLLPP 129
V + L + L + + + + +PG VG LP+ L P
Sbjct: 311 VEVEEELPWIQEKALDLVEFTGSVTQAIPGPRVGPTSLPWILAVP 445
>TC78495 homologue to PIR|S53504|S53504 extensin-like protein S3 - alfalfa,
partial (97%)
Length = 1049
Score = 29.6 bits (65), Expect = 3.1
Identities = 15/51 (29%), Positives = 25/51 (48%)
Frame = +1
Query: 19 HSFSHSTFPSFSSIQLHHQSLSLSLSFPKHHLKLIMSQHLSVSSSPIVEEV 69
H H P LHH L L L P+ HL+ ++ QH + S +++++
Sbjct: 490 HHLHHHPLP-----HLHHSLLPLLLHLPQLHLQQLLLQHSHLLHSHLLQQL 627
>TC76771 homologue to SP|Q42961|PGKH_TOBAC Phosphoglycerate kinase
chloroplast precursor (EC 2.7.2.3). [Common tobacco]
{Nicotiana tabacum}, partial (87%)
Length = 1708
Score = 29.6 bits (65), Expect = 3.1
Identities = 22/46 (47%), Positives = 27/46 (57%)
Frame = -1
Query: 19 HSFSHSTFPSFSSIQLHHQSLSLSLSFPKHHLKLIMSQHLSVSSSP 64
HS S PSF SIQ HH +L S S P L L++S+ LS S+P
Sbjct: 349 HSSQLSQKPSFPSIQ-HHSNLPHS-SSPLTPLLLLLSRLLSSPSTP 218
>TC90143 weakly similar to GP|9280639|gb|AAF86508.1| F21B7.1 {Arabidopsis
thaliana}, partial (5%)
Length = 724
Score = 29.3 bits (64), Expect = 4.0
Identities = 15/47 (31%), Positives = 26/47 (54%)
Frame = +1
Query: 37 QSLSLSLSFPKHHLKLIMSQHLSVSSSPIVEEVNDSSSFDYHQIDPE 83
Q+ S+ SFP H K ++ +V + + ++V DSSS + + PE
Sbjct: 565 QNASIEHSFPDDHCKTLVHDESNVLTEEVTDDV-DSSSSSHEREQPE 702
>TC77043 similar to PIR|S33618|S33618 glutathione peroxidase (EC 1.11.1.9) -
sweet orange, partial (57%)
Length = 1748
Score = 29.3 bits (64), Expect = 4.0
Identities = 15/53 (28%), Positives = 27/53 (50%)
Frame = +3
Query: 29 FSSIQLHHQSLSLSLSFPKHHLKLIMSQHLSVSSSPIVEEVNDSSSFDYHQID 81
F S L H+S+ LSL+FP+++ I + SP + +D ++ +D
Sbjct: 873 FFSYMLGHRSILLSLAFPEYYTNKIKLRWRYFEHSPAINTYSDDVYLVFN*VD 1031
>CB893818 homologue to GP|4582783|emb starch synthase isoform V {Vigna
unguiculata}, partial (33%)
Length = 923
Score = 29.3 bits (64), Expect = 4.0
Identities = 11/29 (37%), Positives = 16/29 (54%)
Frame = -2
Query: 476 TSTAAILMRNGCWHKDHVLTEFGIFGTYL 504
+ AA+ + WH H+L EF FG Y+
Sbjct: 616 SEAAAVPWYSKLWHVKHILAEFNPFGAYM 530
>AL370212
Length = 456
Score = 29.3 bits (64), Expect = 4.0
Identities = 16/37 (43%), Positives = 20/37 (53%)
Frame = -2
Query: 20 SFSHSTFPSFSSIQLHHQSLSLSLSFPKHHLKLIMSQ 56
SFS T P S ++LH S S + SFP H+ SQ
Sbjct: 122 SFSS*TSPQISLLRLHSSSSSFAPSFPLQHVIYSHSQ 12
>TC92128 similar to PIR|B86389|B86389 hypothetical protein AAG29208.1
[imported] - Arabidopsis thaliana, partial (44%)
Length = 523
Score = 29.3 bits (64), Expect = 4.0
Identities = 15/31 (48%), Positives = 17/31 (54%)
Frame = -1
Query: 22 SHSTFPSFSSIQLHHQSLSLSLSFPKHHLKL 52
SH +F S Q HHQS FP HHL+L
Sbjct: 169 SHLQHQNFQSCQNHHQS------FPHHHLEL 95
>BF645392 weakly similar to GP|7630034|em putative protein {Arabidopsis
thaliana}, partial (13%)
Length = 650
Score = 28.9 bits (63), Expect = 5.3
Identities = 20/57 (35%), Positives = 26/57 (45%)
Frame = -3
Query: 23 HSTFPSFSSIQLHHQSLSLSLSFPKHHLKLIMSQHLSVSSSPIVEEVNDSSSFDYHQ 79
H P F QLH ++ L S P HHL + H +V + + N SS YHQ
Sbjct: 384 HQHRPIF*EPQLHQITVKLFFSNPSHHL----TNHTAVKMNHL---RNMSSHLHYHQ 235
>TC77344 similar to GP|4210332|emb|CAA11553.1 2-oxoglutarate dehydrogenase
E2 subunit {Arabidopsis thaliana}, partial (32%)
Length = 741
Score = 28.9 bits (63), Expect = 5.3
Identities = 14/23 (60%), Positives = 15/23 (64%)
Frame = -2
Query: 12 FITSGTSHSFSHSTFPSFSSIQL 34
FITSG SHS HS F F S +L
Sbjct: 722 FITSGESHSIFHSRFIQFMSQKL 654
>TC90647 similar to GP|14517552|gb|AAK62666.1 F17J6.12/F17J6.12 {Arabidopsis
thaliana}, partial (12%)
Length = 1183
Score = 28.5 bits (62), Expect = 6.9
Identities = 18/56 (32%), Positives = 33/56 (58%)
Frame = +1
Query: 17 TSHSFSHSTFPSFSSIQLHHQSLSLSLSFPKHHLKLIMSQHLSVSSSPIVEEVNDS 72
+S + SHST+PSF +++ Q+L S + P + + S+ + +S +E +NDS
Sbjct: 571 SSDNGSHSTYPSFQALE---QNLESSNNSP---VSKLQSKSYTFTSQNSLEIINDS 720
>CB894765
Length = 679
Score = 28.5 bits (62), Expect = 6.9
Identities = 17/33 (51%), Positives = 21/33 (63%), Gaps = 1/33 (3%)
Frame = -3
Query: 12 FITSGTSHSFSHSTFP-SFSSIQLHHQSLSLSL 43
FITS TS + S STFP + S+QL S+ L L
Sbjct: 443 FITSETSSALSSSTFPVTLQSLQLKCFSIGLDL 345
>CA922196 similar to GP|2895758|gb|A phosphatidic acid-preferring
phospholipase A1 {Bos taurus}, partial (1%)
Length = 672
Score = 28.1 bits (61), Expect = 9.0
Identities = 17/48 (35%), Positives = 28/48 (57%), Gaps = 5/48 (10%)
Frame = +3
Query: 36 HQSLSLSLSFPKHHLKLIMSQHLSVSSS--PIVE---EVNDSSSFDYH 78
H +LSL+ FP++H L H+S +S P+V+ ++D +F YH
Sbjct: 384 HANLSLNHQFPRNHELLPHHFHVSNLNSY*PLVQHSSSIHDG*TFQYH 527
>BI269959 similar to GP|20805873|gb| long chain acyl-CoA synthetase 6
{Arabidopsis thaliana}, partial (7%)
Length = 267
Score = 28.1 bits (61), Expect = 9.0
Identities = 23/49 (46%), Positives = 26/49 (52%)
Frame = -1
Query: 14 TSGTSHSFSHSTFPSFSSIQLHHQSLSLSLSFPKHHLKLIMSQHLSVSS 62
TS SHS + S PS S SLSLSLS L L +S LS+SS
Sbjct: 147 TSSLSHSITLSHSPSLSLSLSLSLSLSLSLSL-SLSLSLSLSLSLSLSS 4
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.318 0.135 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,311,996
Number of Sequences: 36976
Number of extensions: 254869
Number of successful extensions: 1791
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 1760
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1787
length of query: 545
length of database: 9,014,727
effective HSP length: 101
effective length of query: 444
effective length of database: 5,280,151
effective search space: 2344387044
effective search space used: 2344387044
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0544.13