
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0544.1
(309 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC81169 similar to GP|20198006|gb|AAD20413.2 expressed protein {... 43 2e-04
BF005391 similar to GP|20198006|gb expressed protein {Arabidopsi... 42 2e-04
TC90685 weakly similar to GP|11994595|dbj|BAB02650. receptor-lik... 28 5.8
TC81634 similar to GP|14423492|gb|AAK62428.1 Unknown protein {Ar... 28 5.8
TC90808 weakly similar to GP|15128387|dbj|BAB62573. contains EST... 28 5.8
TC77282 homologue to GP|17065300|gb|AAL32804.1 Unknown protein {... 27 7.6
TC78973 similar to PIR|D84745|D84745 plastid protein [imported] ... 27 7.6
TC85483 type IIB calcium ATPase MCA5 [Medicago truncatula] 27 7.6
TC84466 similar to PIR|H86389|H86389 hypothetical protein AAG292... 27 10.0
>TC81169 similar to GP|20198006|gb|AAD20413.2 expressed protein {Arabidopsis
thaliana}, partial (53%)
Length = 689
Score = 42.7 bits (99), Expect = 2e-04
Identities = 49/169 (28%), Positives = 72/169 (41%), Gaps = 8/169 (4%)
Frame = +3
Query: 41 LEKLDKELLKGDDRAALALVKDLQGKPDGLRCFGAARQVPQRLYTLDELKLNGIE-AESL 99
L LD + K D A + L + + + + V +R +L EL GI+ AE+L
Sbjct: 171 LNTLDSAIAKKDSNAVKEALDQLS-EIGWAKKWSSQPYVSRRTTSLRELTTLGIKNAENL 347
Query: 100 LSPVDSTLGSIERTLQIAAIAGGLTAWNAFGISPQQL-----FYIS--LGLLFLWTLDTV 152
P S+ G T + GI QL F++ +G + L L
Sbjct: 348 AIP------SVRNDAAFLFTVVGTTGF--LGILAGQLPGDWGFFVPYLIGSISLVVLGIG 503
Query: 153 SYGGGLGNLVVDTIGHSFSKKYHNRVIQHEAGHFLIAYLVGILPKGYTL 201
S GL + + F Y R+ +HEA HFLIAYL+G+ GY+L
Sbjct: 504 STSPGLLQAAISSFSVVFPD-YQERIARHEAAHFLIAYLLGLPILGYSL 647
>BF005391 similar to GP|20198006|gb expressed protein {Arabidopsis thaliana},
partial (52%)
Length = 572
Score = 42.4 bits (98), Expect = 2e-04
Identities = 49/170 (28%), Positives = 76/170 (43%), Gaps = 9/170 (5%)
Frame = +1
Query: 41 LEKLDKELLKGDDRAALALVKDLQGKPDGLRCFGAARQVPQRLYTLDELKLNGIE-AESL 99
L LD + K D A + L + + + + V +R +L EL GI+ AE+L
Sbjct: 43 LNTLDSAIAKKDSNAVKEALDQLS-EIGWAKKWSSQPYVSRRTTSLRELTTLGIKNAENL 219
Query: 100 LSP-VDSTLGSIERTLQIAAIAGGLTAWNAFGISPQQL-----FYIS--LGLLFLWTLDT 151
P V + + ++ + G T + GI QL F++ +G + L L
Sbjct: 220 AIPSVRNDIFVVQAAFLFTVV--GTTGF--LGILAGQLPGDWGFFVPYLIGSISLVVLGI 387
Query: 152 VSYGGGLGNLVVDTIGHSFSKKYHNRVIQHEAGHFLIAYLVGILPKGYTL 201
S GL + + F Y R+ +HEA HFLIAYL+G+ GY+L
Sbjct: 388 GSTSPGLLQAAISSFSVVFPD-YQERIARHEAAHFLIAYLLGLPILGYSL 534
>TC90685 weakly similar to GP|11994595|dbj|BAB02650. receptor-like
serine/threonine kinase {Arabidopsis thaliana}, partial
(13%)
Length = 912
Score = 27.7 bits (60), Expect = 5.8
Identities = 24/97 (24%), Positives = 40/97 (40%), Gaps = 4/97 (4%)
Frame = +1
Query: 34 GVSRRQLLEKLDKELLKGDDRAALALVKDLQGKPDGLRCFGAARQVPQRLYTLDELKLNG 93
G+S+ + L L L G + + +KDL+ K LR +P+ L T+ LK
Sbjct: 61 GISKLEKLTDLRISALSGSEFSPFPQLKDLKLKTLILRNCNINGTLPEYLGTMTTLKNLD 240
Query: 94 IEAESLLSPVDSTLGSIERT----LQIAAIAGGLTAW 126
+ L P+ S + + L + G + AW
Sbjct: 241 LSFNKLSGPIPSNYDPLRKVDYIYLTGNLLTGPVPAW 351
>TC81634 similar to GP|14423492|gb|AAK62428.1 Unknown protein {Arabidopsis
thaliana}, partial (34%)
Length = 888
Score = 27.7 bits (60), Expect = 5.8
Identities = 14/39 (35%), Positives = 21/39 (52%)
Frame = +2
Query: 178 VIQHEAGHFLIAYLVGILPKGYTLSSLDALKKDGSLNVQ 216
++ HE+GHFL A L GI + + L K + NV+
Sbjct: 431 IVVHESGHFLAASLQGIHVSKFAVGFGPILAKFNAKNVE 547
>TC90808 weakly similar to GP|15128387|dbj|BAB62573. contains ESTs
AU096470(S13781) C27591(C52314)~similar to Arabidopsis
thaliana chromosome 4 F24G24., partial (82%)
Length = 944
Score = 27.7 bits (60), Expect = 5.8
Identities = 20/79 (25%), Positives = 33/79 (41%), Gaps = 2/79 (2%)
Frame = +2
Query: 120 AGGLTAWNAFGISPQQLFYISLGLLFLWTLDTVSYGGGLGNLVVDTIGHSFSKKYHNRVI 179
+ G WN G S F+ S L+L L + LVV+ + + N V+
Sbjct: 131 SAGKVEWNNRGFSTFHAFFASFTSLYLLILSDLFKDDSQEKLVVNR-----TSTFSNSVL 295
Query: 180 QHEAGHFL--IAYLVGILP 196
G+FL +A+++ P
Sbjct: 296 SFSTGYFLTDLAFIIWNFP 352
>TC77282 homologue to GP|17065300|gb|AAL32804.1 Unknown protein {Arabidopsis
thaliana}, partial (19%)
Length = 787
Score = 27.3 bits (59), Expect = 7.6
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = -3
Query: 16 PGQLKLVRVRIQCSTTNVGVSRRQLLEKLDKELL 49
PG L LV V +CS + + +++L EKL + +L
Sbjct: 365 PGHLSLVLVVKKCSGLGLSLVKKKLKEKLQERVL 264
>TC78973 similar to PIR|D84745|D84745 plastid protein [imported] -
Arabidopsis thaliana, partial (69%)
Length = 1583
Score = 27.3 bits (59), Expect = 7.6
Identities = 21/66 (31%), Positives = 32/66 (47%)
Frame = -2
Query: 11 GVTSCPGQLKLVRVRIQCSTTNVGVSRRQLLEKLDKELLKGDDRAALALVKDLQGKPDGL 70
G+ S P + +V V ++ V RR+L DD AL ++ DL+ K G+
Sbjct: 769 GLESAPATV-VV*VEVEVEVEVVVEKRRRLK----------DDAIALVVMMDLKEKEMGI 623
Query: 71 RCFGAA 76
R FGA+
Sbjct: 622 RVFGAS 605
>TC85483 type IIB calcium ATPase MCA5 [Medicago truncatula]
Length = 2541
Score = 27.3 bits (59), Expect = 7.6
Identities = 22/93 (23%), Positives = 40/93 (42%)
Frame = +2
Query: 167 GHSFSKKYHNRVIQHEAGHFLIAYLVGILPKGYTLSSLDALKKDGSLNVQAGSAFVDFEF 226
G+ S ++ F++ + + KG T+ SLD D LN +AFV +
Sbjct: 1700 GNFISNVMWRNILGQSLYQFMVIWF--LQSKGKTIFSLDGPNSDLVLNTLIFNAFVFCQV 1873
Query: 227 LEEVNAGKVSAKTLNKFSCIALAGVCTEYLIYG 259
E+N+ ++ + +N F G+ Y+ G
Sbjct: 1874 FNEINSREM--EKINVFK-----GILDNYVFVG 1951
>TC84466 similar to PIR|H86389|H86389 hypothetical protein AAG29222.1
[imported] - Arabidopsis thaliana, partial (4%)
Length = 732
Score = 26.9 bits (58), Expect = 10.0
Identities = 20/52 (38%), Positives = 24/52 (45%)
Frame = +1
Query: 50 KGDDRAALALVKDLQGKPDGLRCFGAARQVPQRLYTLDELKLNGIEAESLLS 101
KG +R + K L K GL FG Q QRL +NGI SL+S
Sbjct: 346 KGYNRMKIWHGKRLSSKMYGLELFGFKNQFIQRLLRELVSDINGIVDRSLVS 501
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.321 0.139 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,909,072
Number of Sequences: 36976
Number of extensions: 112322
Number of successful extensions: 444
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 444
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 444
length of query: 309
length of database: 9,014,727
effective HSP length: 96
effective length of query: 213
effective length of database: 5,465,031
effective search space: 1164051603
effective search space used: 1164051603
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0544.1