Lotus
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0544.1
         (309 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC81169 similar to GP|20198006|gb|AAD20413.2 expressed protein {...    43  2e-04
BF005391 similar to GP|20198006|gb expressed protein {Arabidopsi...    42  2e-04
TC90685 weakly similar to GP|11994595|dbj|BAB02650. receptor-lik...    28  5.8
TC81634 similar to GP|14423492|gb|AAK62428.1 Unknown protein {Ar...    28  5.8
TC90808 weakly similar to GP|15128387|dbj|BAB62573. contains EST...    28  5.8
TC77282 homologue to GP|17065300|gb|AAL32804.1 Unknown protein {...    27  7.6
TC78973 similar to PIR|D84745|D84745 plastid protein [imported] ...    27  7.6
TC85483 type IIB calcium ATPase MCA5 [Medicago truncatula]             27  7.6
TC84466 similar to PIR|H86389|H86389 hypothetical protein AAG292...    27  10.0

>TC81169 similar to GP|20198006|gb|AAD20413.2 expressed protein {Arabidopsis
           thaliana}, partial (53%)
          Length = 689

 Score = 42.7 bits (99), Expect = 2e-04
 Identities = 49/169 (28%), Positives = 72/169 (41%), Gaps = 8/169 (4%)
 Frame = +3

Query: 41  LEKLDKELLKGDDRAALALVKDLQGKPDGLRCFGAARQVPQRLYTLDELKLNGIE-AESL 99
           L  LD  + K D  A    +  L  +    + + +   V +R  +L EL   GI+ AE+L
Sbjct: 171 LNTLDSAIAKKDSNAVKEALDQLS-EIGWAKKWSSQPYVSRRTTSLRELTTLGIKNAENL 347

Query: 100 LSPVDSTLGSIERTLQIAAIAGGLTAWNAFGISPQQL-----FYIS--LGLLFLWTLDTV 152
             P      S+           G T +   GI   QL     F++   +G + L  L   
Sbjct: 348 AIP------SVRNDAAFLFTVVGTTGF--LGILAGQLPGDWGFFVPYLIGSISLVVLGIG 503

Query: 153 SYGGGLGNLVVDTIGHSFSKKYHNRVIQHEAGHFLIAYLVGILPKGYTL 201
           S   GL    + +    F   Y  R+ +HEA HFLIAYL+G+   GY+L
Sbjct: 504 STSPGLLQAAISSFSVVFPD-YQERIARHEAAHFLIAYLLGLPILGYSL 647


>BF005391 similar to GP|20198006|gb expressed protein {Arabidopsis thaliana},
           partial (52%)
          Length = 572

 Score = 42.4 bits (98), Expect = 2e-04
 Identities = 49/170 (28%), Positives = 76/170 (43%), Gaps = 9/170 (5%)
 Frame = +1

Query: 41  LEKLDKELLKGDDRAALALVKDLQGKPDGLRCFGAARQVPQRLYTLDELKLNGIE-AESL 99
           L  LD  + K D  A    +  L  +    + + +   V +R  +L EL   GI+ AE+L
Sbjct: 43  LNTLDSAIAKKDSNAVKEALDQLS-EIGWAKKWSSQPYVSRRTTSLRELTTLGIKNAENL 219

Query: 100 LSP-VDSTLGSIERTLQIAAIAGGLTAWNAFGISPQQL-----FYIS--LGLLFLWTLDT 151
             P V + +  ++       +  G T +   GI   QL     F++   +G + L  L  
Sbjct: 220 AIPSVRNDIFVVQAAFLFTVV--GTTGF--LGILAGQLPGDWGFFVPYLIGSISLVVLGI 387

Query: 152 VSYGGGLGNLVVDTIGHSFSKKYHNRVIQHEAGHFLIAYLVGILPKGYTL 201
            S   GL    + +    F   Y  R+ +HEA HFLIAYL+G+   GY+L
Sbjct: 388 GSTSPGLLQAAISSFSVVFPD-YQERIARHEAAHFLIAYLLGLPILGYSL 534


>TC90685 weakly similar to GP|11994595|dbj|BAB02650. receptor-like
           serine/threonine kinase {Arabidopsis thaliana}, partial
           (13%)
          Length = 912

 Score = 27.7 bits (60), Expect = 5.8
 Identities = 24/97 (24%), Positives = 40/97 (40%), Gaps = 4/97 (4%)
 Frame = +1

Query: 34  GVSRRQLLEKLDKELLKGDDRAALALVKDLQGKPDGLRCFGAARQVPQRLYTLDELKLNG 93
           G+S+ + L  L    L G + +    +KDL+ K   LR       +P+ L T+  LK   
Sbjct: 61  GISKLEKLTDLRISALSGSEFSPFPQLKDLKLKTLILRNCNINGTLPEYLGTMTTLKNLD 240

Query: 94  IEAESLLSPVDSTLGSIERT----LQIAAIAGGLTAW 126
           +    L  P+ S    + +     L    + G + AW
Sbjct: 241 LSFNKLSGPIPSNYDPLRKVDYIYLTGNLLTGPVPAW 351


>TC81634 similar to GP|14423492|gb|AAK62428.1 Unknown protein {Arabidopsis
           thaliana}, partial (34%)
          Length = 888

 Score = 27.7 bits (60), Expect = 5.8
 Identities = 14/39 (35%), Positives = 21/39 (52%)
 Frame = +2

Query: 178 VIQHEAGHFLIAYLVGILPKGYTLSSLDALKKDGSLNVQ 216
           ++ HE+GHFL A L GI    + +     L K  + NV+
Sbjct: 431 IVVHESGHFLAASLQGIHVSKFAVGFGPILAKFNAKNVE 547


>TC90808 weakly similar to GP|15128387|dbj|BAB62573. contains ESTs
           AU096470(S13781) C27591(C52314)~similar to Arabidopsis
           thaliana chromosome 4  F24G24., partial (82%)
          Length = 944

 Score = 27.7 bits (60), Expect = 5.8
 Identities = 20/79 (25%), Positives = 33/79 (41%), Gaps = 2/79 (2%)
 Frame = +2

Query: 120 AGGLTAWNAFGISPQQLFYISLGLLFLWTLDTVSYGGGLGNLVVDTIGHSFSKKYHNRVI 179
           + G   WN  G S    F+ S   L+L  L  +        LVV+      +  + N V+
Sbjct: 131 SAGKVEWNNRGFSTFHAFFASFTSLYLLILSDLFKDDSQEKLVVNR-----TSTFSNSVL 295

Query: 180 QHEAGHFL--IAYLVGILP 196
               G+FL  +A+++   P
Sbjct: 296 SFSTGYFLTDLAFIIWNFP 352


>TC77282 homologue to GP|17065300|gb|AAL32804.1 Unknown protein {Arabidopsis
           thaliana}, partial (19%)
          Length = 787

 Score = 27.3 bits (59), Expect = 7.6
 Identities = 13/34 (38%), Positives = 21/34 (61%)
 Frame = -3

Query: 16  PGQLKLVRVRIQCSTTNVGVSRRQLLEKLDKELL 49
           PG L LV V  +CS   + + +++L EKL + +L
Sbjct: 365 PGHLSLVLVVKKCSGLGLSLVKKKLKEKLQERVL 264


>TC78973 similar to PIR|D84745|D84745 plastid protein [imported] -
           Arabidopsis thaliana, partial (69%)
          Length = 1583

 Score = 27.3 bits (59), Expect = 7.6
 Identities = 21/66 (31%), Positives = 32/66 (47%)
 Frame = -2

Query: 11  GVTSCPGQLKLVRVRIQCSTTNVGVSRRQLLEKLDKELLKGDDRAALALVKDLQGKPDGL 70
           G+ S P  + +V V ++     V   RR+L           DD  AL ++ DL+ K  G+
Sbjct: 769 GLESAPATV-VV*VEVEVEVEVVVEKRRRLK----------DDAIALVVMMDLKEKEMGI 623

Query: 71  RCFGAA 76
           R FGA+
Sbjct: 622 RVFGAS 605


>TC85483 type IIB calcium ATPase MCA5 [Medicago truncatula]
          Length = 2541

 Score = 27.3 bits (59), Expect = 7.6
 Identities = 22/93 (23%), Positives = 40/93 (42%)
 Frame = +2

Query: 167  GHSFSKKYHNRVIQHEAGHFLIAYLVGILPKGYTLSSLDALKKDGSLNVQAGSAFVDFEF 226
            G+  S      ++      F++ +   +  KG T+ SLD    D  LN    +AFV  + 
Sbjct: 1700 GNFISNVMWRNILGQSLYQFMVIWF--LQSKGKTIFSLDGPNSDLVLNTLIFNAFVFCQV 1873

Query: 227  LEEVNAGKVSAKTLNKFSCIALAGVCTEYLIYG 259
              E+N+ ++  + +N F      G+   Y+  G
Sbjct: 1874 FNEINSREM--EKINVFK-----GILDNYVFVG 1951


>TC84466 similar to PIR|H86389|H86389 hypothetical protein AAG29222.1
           [imported] - Arabidopsis thaliana, partial (4%)
          Length = 732

 Score = 26.9 bits (58), Expect = 10.0
 Identities = 20/52 (38%), Positives = 24/52 (45%)
 Frame = +1

Query: 50  KGDDRAALALVKDLQGKPDGLRCFGAARQVPQRLYTLDELKLNGIEAESLLS 101
           KG +R  +   K L  K  GL  FG   Q  QRL       +NGI   SL+S
Sbjct: 346 KGYNRMKIWHGKRLSSKMYGLELFGFKNQFIQRLLRELVSDINGIVDRSLVS 501


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.321    0.139    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,909,072
Number of Sequences: 36976
Number of extensions: 112322
Number of successful extensions: 444
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 444
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 444
length of query: 309
length of database: 9,014,727
effective HSP length: 96
effective length of query: 213
effective length of database: 5,465,031
effective search space: 1164051603
effective search space used: 1164051603
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 58 (26.9 bits)


Lotus: description of TM0544.1