BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0520.11
(117 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC92808 similar to GP|9759609|dbj|BAB11551.1 gene_id:MNA5.1~unkn... 29 0.23
TC90096 similar to PIR|T47832|T47832 hypothetical protein T2O9.6... 29 0.23
TC81182 29 0.30
TC80645 similar to PIR|T50983|T50983 probable pleiotropic regula... 28 0.51
BG644436 28 0.51
TC87886 weakly similar to GP|5262202|emb|CAB45799.1 nodulin-like... 28 0.51
TC81676 similar to PIR|T05707|T05707 phosphate transport protein... 28 0.51
BQ149637 similar to PIR|D85135|D85 hypothetical protein AT4g1261... 28 0.67
BF638076 homologue to GP|16769034|gb| LD15209p {Drosophila melan... 26 2.0
TC92067 similar to GP|7298791|gb|AAF54000.1| CG1062 gene product... 26 2.0
TC81991 similar to PIR|D96545|D96545 probable DNA polymerase A f... 26 2.0
TC77892 similar to GP|14861486|gb|AAK73693.1 succinate dehydroge... 26 2.6
TC85148 homologue to PIR|T06827|T06827 lipoxygenase (EC 1.13.11.... 26 2.6
TC84085 similar to PIR|D84436|D84436 hypothetical protein At2g02... 26 2.6
TC82049 GP|19571116|dbj|BAB86540. contains EST D41521(S4063)~unk... 25 3.3
TC92853 similar to GP|20268748|gb|AAM14077.1 unknown protein {Ar... 25 3.3
BQ135952 25 3.3
TC78530 similar to PIR|T07061|T07061 L-lactate dehydrogenase (EC... 25 3.3
BG451711 25 4.4
TC79077 similar to GP|17529252|gb|AAL38853.1 unknown protein {Ar... 25 4.4
>TC92808 similar to GP|9759609|dbj|BAB11551.1 gene_id:MNA5.1~unknown protein
{Arabidopsis thaliana}, partial (6%)
Length = 673
Score = 29.3 bits (64), Expect = 0.23
Identities = 20/59 (33%), Positives = 31/59 (51%), Gaps = 3/59 (5%)
Frame = +3
Query: 46 SSSSSSTSQCDLPKGQGGNVAKIKAEHRRVHSTGNIPYPGSSQPKL---VRSSGMRRDW 101
SS + +S+ +GQGG+ +K+KAE + V S + GSS + SSG+ W
Sbjct: 315 SSREAISSKYAAIRGQGGSSSKLKAEEKNVASYSGLD-EGSSNSRNSAGAPSSGLASTW 488
>TC90096 similar to PIR|T47832|T47832 hypothetical protein T2O9.60 -
Arabidopsis thaliana, partial (13%)
Length = 676
Score = 29.3 bits (64), Expect = 0.23
Identities = 16/37 (43%), Positives = 23/37 (61%)
Frame = -3
Query: 34 VDRNWSGNLIASSSSSSSTSQCDLPKGQGGNVAKIKA 70
+ R S LI+SSSSSSS+S +P Q G +I++
Sbjct: 578 ISRITSPPLISSSSSSSSSSSSTIPHRQRGQEERIRS 468
>TC81182
Length = 917
Score = 28.9 bits (63), Expect = 0.30
Identities = 19/66 (28%), Positives = 30/66 (44%)
Frame = +1
Query: 10 QNLQRTDSFGEFFTEKEYKEVCKKVDRNWSGNLIASSSSSSSTSQCDLPKGQGGNVAKIK 69
+N+ D+ F EK+ E+ + + W LIAS ++ +S LPKG N K
Sbjct: 280 RNMSCNDNNESCFVEKKLSEMGEDSIKRWKSMLIASDFDNTESS-TKLPKGVKENTLMAK 456
Query: 70 AEHRRV 75
R+
Sbjct: 457 LLEARL 474
>TC80645 similar to PIR|T50983|T50983 probable pleiotropic regulator 1
(PLRG1) [imported] - Neurospora crassa, partial (41%)
Length = 1141
Score = 28.1 bits (61), Expect = 0.51
Identities = 14/33 (42%), Positives = 19/33 (57%)
Frame = -1
Query: 63 GNVAKIKAEHRRVHSTGNIPYPGSSQPKLVRSS 95
GN A + +H + S +IPYPG QP+L S
Sbjct: 94 GNSACMTRQHVNIASRSHIPYPG--QPRLCHQS 2
>BG644436
Length = 650
Score = 28.1 bits (61), Expect = 0.51
Identities = 15/51 (29%), Positives = 25/51 (48%)
Frame = +2
Query: 19 GEFFTEKEYKEVCKKVDRNWSGNLIASSSSSSSTSQCDLPKGQGGNVAKIK 69
G F +K+++ V K ++W G SSS+S Q + K + + K K
Sbjct: 239 GWFQVKKKHRNVSKFSLQSWMGGFTGKSSSTSQEKQQSMNKKENNSHGKQK 391
>TC87886 weakly similar to GP|5262202|emb|CAB45799.1 nodulin-like protein
{Arabidopsis thaliana}, partial (79%)
Length = 1337
Score = 28.1 bits (61), Expect = 0.51
Identities = 16/42 (38%), Positives = 22/42 (52%), Gaps = 3/42 (7%)
Frame = -1
Query: 53 SQCDLPKGQGGNVAKIKAEHRRVHS---TGNIPYPGSSQPKL 91
++C KG N I EH+ +HS TG+ Y S +PKL
Sbjct: 251 NECKGSKGNCSNDITINNEHKTIHSHVETGHGEYVCSCKPKL 126
>TC81676 similar to PIR|T05707|T05707 phosphate transport protein G7
mitochondrial - soybean, partial (81%)
Length = 1384
Score = 28.1 bits (61), Expect = 0.51
Identities = 18/53 (33%), Positives = 25/53 (46%), Gaps = 4/53 (7%)
Frame = +3
Query: 5 CLTCSQNLQRT-DSFGEFFTEKEYKEVCK---KVDRNWSGNLIASSSSSSSTS 53
CL+C + + DSF + EKEYK V R W +++ S S TS
Sbjct: 183 CLSCGSGISKVYDSFSQ*IREKEYKVVLS*LLSSLRRWWKHMLRLHSYGSYTS 341
>BQ149637 similar to PIR|D85135|D85 hypothetical protein AT4g12610 [imported]
- Arabidopsis thaliana, partial (15%)
Length = 538
Score = 27.7 bits (60), Expect = 0.67
Identities = 18/46 (39%), Positives = 25/46 (54%)
Frame = -1
Query: 46 SSSSSSTSQCDLPKGQGGNVAKIKAEHRRVHSTGNIPYPGSSQPKL 91
SSSSSS+S C + G G + AK S+G++P SS K+
Sbjct: 286 SSSSSSSSSCLISGGAGTSGAKSSL------SSGSLPTASSSSVKI 167
>BF638076 homologue to GP|16769034|gb| LD15209p {Drosophila melanogaster},
partial (7%)
Length = 588
Score = 26.2 bits (56), Expect = 2.0
Identities = 17/49 (34%), Positives = 26/49 (52%)
Frame = +2
Query: 32 KKVDRNWSGNLIASSSSSSSTSQCDLPKGQGGNVAKIKAEHRRVHSTGN 80
+K+ W +SSSSSSS+S G ++K+K H R++S N
Sbjct: 5 QKIMMKWGAKKPSSSSSSSSSSSTS--SGSFSWLSKLK--HMRINSDSN 139
>TC92067 similar to GP|7298791|gb|AAF54000.1| CG1062 gene product
{Drosophila melanogaster}, partial (6%)
Length = 540
Score = 26.2 bits (56), Expect = 2.0
Identities = 13/29 (44%), Positives = 18/29 (61%)
Frame = +2
Query: 33 KVDRNWSGNLIASSSSSSSTSQCDLPKGQ 61
K+ + S + SSS SSS+S C L KG+
Sbjct: 182 KIKKMGSSSSKTSSSCSSSSSSCSLRKGR 268
>TC81991 similar to PIR|D96545|D96545 probable DNA polymerase A family
protein [imported] - Arabidopsis thaliana, partial
(32%)
Length = 1543
Score = 26.2 bits (56), Expect = 2.0
Identities = 24/65 (36%), Positives = 30/65 (45%)
Frame = +1
Query: 17 SFGEFFTEKEYKEVCKKVDRNWSGNLIASSSSSSSTSQCDLPKGQGGNVAKIKAEHRRVH 76
+F F TEKE +E C + L SS +S S LP QG N I + RVH
Sbjct: 10 AFSAFPTEKEGREACHAI-----AALCEVSSINSLISNFILPL-QGHN---ISGKDNRVH 162
Query: 77 STGNI 81
+ NI
Sbjct: 163CSLNI 177
>TC77892 similar to GP|14861486|gb|AAK73693.1 succinate dehydrogenase
subunit 3 {Glycine max}, partial (92%)
Length = 1148
Score = 25.8 bits (55), Expect = 2.6
Identities = 15/55 (27%), Positives = 24/55 (43%)
Frame = +1
Query: 36 RNWSGNLIASSSSSSSTSQCDLPKGQGGNVAKIKAEHRRVHSTGNIPYPGSSQPK 90
R+ L SSSSS S++ +P+ G A + +T +PY + K
Sbjct: 79 RSTKSKLFLSSSSSLSSTFSSIPRSTGSLSPAAPAAEQFHRTTTTLPYADQNSTK 243
>TC85148 homologue to PIR|T06827|T06827 lipoxygenase (EC 1.13.11.12) -
garden pea, complete
Length = 2962
Score = 25.8 bits (55), Expect = 2.6
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = +1
Query: 27 YKEVCKKVDRNWSGNLIASSSSS 49
YK+ CK +W GN+ +SSS
Sbjct: 283 YKD*CKWKGTSWKGNIFEQTSSS 351
>TC84085 similar to PIR|D84436|D84436 hypothetical protein At2g02410
[imported] - Arabidopsis thaliana, partial (23%)
Length = 596
Score = 25.8 bits (55), Expect = 2.6
Identities = 18/55 (32%), Positives = 29/55 (52%)
Frame = +3
Query: 41 NLIASSSSSSSTSQCDLPKGQGGNVAKIKAEHRRVHSTGNIPYPGSSQPKLVRSS 95
N ASSSSSSS+S C + K V K+ ++ S+ + P P P++ ++
Sbjct: 129 NYNASSSSSSSSSCCYVKKRHSMIVYSSKSS-KKNDSSNSQPTPPPPPPRITSNA 290
>TC82049 GP|19571116|dbj|BAB86540. contains EST D41521(S4063)~unknown
protein {Oryza sativa (japonica cultivar-group)},
partial (9%)
Length = 795
Score = 25.4 bits (54), Expect = 3.3
Identities = 28/112 (25%), Positives = 45/112 (40%), Gaps = 6/112 (5%)
Frame = +1
Query: 1 MSLNCLTCS--QNLQRTDSFGEFFTEKEYKEVCKKVDRNWSGNLIASSSSSSSTSQCDLP 58
MSL CL C +NL + SF + + + C + + L + S
Sbjct: 274 MSLACLVCHSVENLSPSHSFRRSVSNSDNEGRCYAIANCLTRKLSVQPPTVHSFL----- 438
Query: 59 KGQGGNVAKIKAEHRRVHSTGNIPYPGSSQPKLVRSSGMRR----DWSFENL 106
+ +K+ + S IP P P+LVRS +RR DW+F+ +
Sbjct: 439 ---AASTSKVTPQP----SNTEIPGP----PRLVRSRAVRRDIVQDWNFDEV 561
>TC92853 similar to GP|20268748|gb|AAM14077.1 unknown protein {Arabidopsis
thaliana}, partial (21%)
Length = 761
Score = 25.4 bits (54), Expect = 3.3
Identities = 16/54 (29%), Positives = 25/54 (45%)
Frame = -2
Query: 39 SGNLIASSSSSSSTSQCDLPKGQGGNVAKIKAEHRRVHSTGNIPYPGSSQPKLV 92
S +L++SSSSSSS+S + + STG + + G KL+
Sbjct: 442 SSSLVSSSSSSSSSSSSSFSLPSSSLSLSLPLVSLPLVSTGILGFGGEGATKLI 281
>BQ135952
Length = 861
Score = 25.4 bits (54), Expect = 3.3
Identities = 11/31 (35%), Positives = 18/31 (57%)
Frame = -1
Query: 9 SQNLQRTDSFGEFFTEKEYKEVCKKVDRNWS 39
SQ L+ SFG+ F++ EY+ V +W+
Sbjct: 363 SQFLESFLSFGKLFSQYEYRRSAHTVQDSWA 271
>TC78530 similar to PIR|T07061|T07061 L-lactate dehydrogenase (EC 1.1.1.27)
chain 1 - tomato, complete
Length = 1487
Score = 25.4 bits (54), Expect = 3.3
Identities = 16/44 (36%), Positives = 26/44 (58%)
Frame = -1
Query: 71 EHRRVHSTGNIPYPGSSQPKLVRSSGMRRDWSFENLAENQDQSV 114
EH+R + + P+P S++PK V S +D S E+ A + +SV
Sbjct: 602 EHQRG*TR*SEPFPASNEPKEV*SP*TEKDSSEED*A*TRHRSV 471
>BG451711
Length = 207
Score = 25.0 bits (53), Expect = 4.4
Identities = 15/42 (35%), Positives = 21/42 (49%)
Frame = +1
Query: 9 SQNLQRTDSFGEFFTEKEYKEVCKKVDRNWSGNLIASSSSSS 50
S + Q T+ F T KEYK + K ++ I S+SSS
Sbjct: 16 SSSSQNTNFFNN*IT*KEYKSIIKMKEKKKKKA*ICISNSSS 141
>TC79077 similar to GP|17529252|gb|AAL38853.1 unknown protein {Arabidopsis
thaliana}, partial (38%)
Length = 2200
Score = 25.0 bits (53), Expect = 4.4
Identities = 13/37 (35%), Positives = 20/37 (53%)
Frame = -1
Query: 18 FGEFFTEKEYKEVCKKVDRNWSGNLIASSSSSSSTSQ 54
F +F + +C +RN+ L SSSSS +TS+
Sbjct: 889 FFDFLDSLSSRSLCATCERNFVTLLNCSSSSSHTTSR 779
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.311 0.125 0.374
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,311,236
Number of Sequences: 36976
Number of extensions: 67265
Number of successful extensions: 337
Number of sequences better than 10.0: 70
Number of HSP's better than 10.0 without gapping: 329
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 335
length of query: 117
length of database: 9,014,727
effective HSP length: 93
effective length of query: 24
effective length of database: 5,575,959
effective search space: 133823016
effective search space used: 133823016
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 50 (23.9 bits)
Lotus: description of TM0520.11