BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0490.11
(418 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC86737 weakly similar to GP|6683624|dbj|BAA89272.1 Pol {Alterna... 139 2e-33
BG644693 weakly similar to GP|18767374|g Putative 22 kDa kafirin... 82 5e-16
BG587145 similar to PIR|H86337|H8 protein F5M15.26 [imported] - ... 55 4e-08
BG586326 similar to PIR|G84493|G8 probable retroelement pol poly... 39 0.004
BG644740 similar to PIR|A84460|A84 probable retroelement pol pol... 36 0.032
BI309716 weakly similar to PIR|G96722|G9 hypothetical protein F2... 34 0.12
AL370071 similar to GP|15795135|dbj transposase-like protein {Ar... 30 1.3
BQ141686 homologue to PIR|S34666|S346 glycine-rich protein - com... 30 2.3
AL374846 weakly similar to PIR|T05449|T054 hypothetical protein ... 29 2.9
TC83085 weakly similar to GP|11994101|dbj|BAB01104. gb|AAD39678.... 29 2.9
BF004404 similar to GP|21928159|gb AT4g02010/T10M13_2 {Arabidops... 29 3.9
BG457911 GP|3047314|gb| ubiquitin fusion protein {Magnaporthe gr... 28 5.0
TC82766 similar to GP|3128183|gb|AAC16087.1| unknown protein {Ar... 28 8.6
TC80484 similar to GP|11994729|dbj|BAB03045. gene_id:F5N5.17~pir... 28 8.6
>TC86737 weakly similar to GP|6683624|dbj|BAA89272.1 Pol {Alternaria
alternata}, partial (21%)
Length = 1540
Score = 139 bits (350), Expect = 2e-33
Identities = 103/347 (29%), Positives = 167/347 (47%), Gaps = 6/347 (1%)
Frame = +1
Query: 1 MTPSELRAAQEECQQLIKQGLIEPTKSEWACTAFYVNKRSEQVRQKKRLVVDYRPLNQFI 60
M+ EL ++ + L+ +G I+ + S +V K +R VDYR LN
Sbjct: 487 MSRQELLVLKKTLEDLLDKGFIKASGSAAGAPVLFVRKPGGGIR----FCVDYRALNAIT 654
Query: 61 KDDKFPLPKIYSLYSYIKNAHIFSKFDMKSGFWQLGLDPKDRPKTAFCIPNAHYQWTVLP 120
K D++PLP I + A F+K D+ + F ++ + +D+ KTAF ++W V P
Sbjct: 655 KKDRYPLPLISETLRRVAGARWFTKLDVVAAFHKMRIKDEDQEKTAFRTRYGLFEWIVCP 834
Query: 121 FGLKVAPSLFQKAMTKIFEPILHTSL-VYIDDVLLF-SHDEESHKKFLQQFYDICSKHGV 178
FGL AP+ FQ+ + K L + YIDDVL++ + ++ H+ +++ + G+
Sbjct: 835 FGLTGAPATFQRYINKTLHEFLDDFVTAYIDDVLIYTTGSKKDHEAQVRRVLRRLADAGL 1014
Query: 179 MLSSRKSQIATKEVDFLGMHFTEGKFVP-QPHIVEELPKFPDEGLTIKQIQQFLGILNYI 237
L +K + + V ++G T GK V P + + + G ++K + FLG NY
Sbjct: 1015SLDPKKCEFSVTTVKYVGFILTAGKGVSCDPLKLAAIRDWLPPG-SVKGARSFLGFCNYY 1191
Query: 238 RDFIPHVSKYTSKLSKMLRKNAP-PWGKEQTEAIKFLKKAAQNPPPLKI-PGDGKRILQT 295
+DFIP S+ T L+++ RK+ P WG EQ A LK+ P L++ + ++T
Sbjct: 1192KDFIPGYSEITEPLTRLTRKDFPFRWGAEQEAAFTKLKRLFAEEPVLRMFDPEAVTTVET 1371
Query: 296 DASDHYWAAVLIEEI-DGQKLYCGHASGQFKDAELHYHTTFKETLAV 341
D S VL +E G S + AE +Y KE LAV
Sbjct: 1372DCSGFALGGVLTQEDGTGAAHPVAFHSQRLSPAEYNYPIHDKELLAV 1512
>BG644693 weakly similar to GP|18767374|g Putative 22 kDa kafirin cluster;
Ty3-Gypsy type {Oryza sativa}, partial (15%)
Length = 716
Score = 81.6 bits (200), Expect = 5e-16
Identities = 54/204 (26%), Positives = 100/204 (48%), Gaps = 1/204 (0%)
Frame = +2
Query: 1 MTPSELRAAQEECQQLIKQGLIEPTKSEWACTAFYVNKRSEQVRQKKRLVVDYRPLNQFI 60
+ P +L+ + + + L+++G I+P+ ++ K+ +R +DY LN
Sbjct: 95 INPLKLKVLKLQLKDLLEKGFIQPSIYP*GVVVLFLKKKDGFLRMS----IDYPQLNNVN 262
Query: 61 KDDKFPLPKIYSLYSYIKNAHIFSKFDMKSGFWQLGLDPKDRPKTAFCIPNAHYQWTVLP 120
K+PLP I L+ ++ + F K D++ G Q + +D PKTAF I HY+ V+
Sbjct: 263 IKIKYPLPLIDELFDNLQGSKWFFKIDLRLG*HQHRVIGEDVPKTAFRIRYGHYEILVMS 442
Query: 121 FGLKVAPSLFQKAMTKIFEPILHT-SLVYIDDVLLFSHDEESHKKFLQQFYDICSKHGVM 179
FG P F + M ++F+ L + +V+ +D+L++S +E H+ L+ + G+
Sbjct: 443 FG*TNPPMAFMELMNRVFQDYLDSLVIVFSNDILIYSKNENEHENHLRLALKVLKDIGLC 622
Query: 180 LSSRKSQIATKEVDFLGMHFTEGK 203
S + EV F +H G+
Sbjct: 623 QISYV*ILV--EVGFFSLHVISGE 688
>BG587145 similar to PIR|H86337|H8 protein F5M15.26 [imported] - Arabidopsis
thaliana, partial (13%)
Length = 763
Score = 55.5 bits (132), Expect = 4e-08
Identities = 37/137 (27%), Positives = 65/137 (47%), Gaps = 1/137 (0%)
Frame = +2
Query: 97 LDPKDRPKTAFCIPNAHYQWTVLPFGLKVAPSLFQKAMTKIF-EPILHTSLVYIDDVLLF 155
+ P D KTAF Y + V+PFGLK A S +Q+ + ++F + + +T VYIDD+L+
Sbjct: 2 MHPDDLEKTAFITDRGTYCYKVMPFGLKNAGSTYQRLVNRMFADKLGNTMEVYIDDMLVK 181
Query: 156 SHDEESHKKFLQQFYDICSKHGVMLSSRKSQIATKEVDFLGMHFTEGKFVPQPHIVEELP 215
S H L++ + ++ + L+ K +FLG T+ P + +
Sbjct: 182 SLRATDHLNHLKE*FKTLDEYIMKLNPAKCTFGVTSGEFLGYIVTQQGIEVNPKQITAIL 361
Query: 216 KFPDEGLTIKQIQQFLG 232
P +++Q+ G
Sbjct: 362 DLPSP-KNSREVQRLTG 409
>BG586326 similar to PIR|G84493|G8 probable retroelement pol polyprotein
[imported] - Arabidopsis thaliana, partial (13%)
Length = 736
Score = 38.9 bits (89), Expect = 0.004
Identities = 36/136 (26%), Positives = 58/136 (42%), Gaps = 4/136 (2%)
Frame = +2
Query: 281 PPLKIPGDGKRILQTDASDHYWAAVLIEEIDGQKLYCGHASGQFKDAELHYHTTFKETLA 340
P L +P ++ TDAS VL + + +AS Q + E +Y T E A
Sbjct: 17 PILVLPELITYVVYTDASITGLGCVLTQH----EKVIAYASRQLRKHEGNYPTHDLEMAA 184
Query: 341 VKYGIKRFDFHLRGHHFEVQIDNSSFPKMLEFKNKTPPNPQI-LRLKEWFSL---YDFSV 396
V + +K + +L G ++ D+ S + P++ LR + W YD +
Sbjct: 185 VVFALKIWRSYLYGAKVQIHTDHKSLKYIFT-------QPELNLRQRRWMEFVADYDLDI 343
Query: 397 KHVQGSKNLIPDYLSR 412
+ G NL+ D LSR
Sbjct: 344 TYYPGKANLVADALSR 391
>BG644740 similar to PIR|A84460|A84 probable retroelement pol polyprotein
[imported] - Arabidopsis thaliana, partial (4%)
Length = 754
Score = 35.8 bits (81), Expect = 0.032
Identities = 16/48 (33%), Positives = 27/48 (55%)
Frame = -1
Query: 48 RLVVDYRPLNQFIKDDKFPLPKIYSLYSYIKNAHIFSKFDMKSGFWQL 95
R+ +DYR N+ +K+PLP+I +L+ I+ F D++ G L
Sbjct: 199 RMCIDYRQFNKVTTKNKYPLPRIDNLFDKIQEDCYF*NIDLRLGIINL 56
>BI309716 weakly similar to PIR|G96722|G9 hypothetical protein F20P5.25
[imported] - Arabidopsis thaliana, partial (10%)
Length = 744
Score = 33.9 bits (76), Expect = 0.12
Identities = 48/201 (23%), Positives = 82/201 (39%), Gaps = 28/201 (13%)
Frame = +2
Query: 104 KTAFCIPNAHYQW------TVLPFGLKVAPSLFQKAMTKIFEPILHTSLVYIDDVLLFSH 157
K+ + + A QW +++ FG + S F TK + T LVY+DD++L +
Sbjct: 35 KSIYGLKQASRQWYSKLSESLISFGYLQSSSDFS-LFTKFKDSSFTTLLVYVDDIVLAGN 211
Query: 158 D--EESHKK-FLQQFYDI---------------CSKHGVMLSSRKSQIATKEVDFLGMHF 199
D E H K FL + I SK G++L+ RK + E G
Sbjct: 212 DISEIQHVKCFLIDRFKIKDLGSLRYFLGLEVARSKQGILLNQRKYTLELLEDS--GNLA 385
Query: 200 TEGKFVPQPHIVE----ELPKFPDEGLTIKQIQQFLGILNYIRDFIPHVSKYTSKLSKML 255
+ P ++ + P + DE Q ++ +G L Y+ P +S +LS+ +
Sbjct: 386 VKSTLTPYDISLKLHNSDSPLYNDE----TQYRRLIGKLIYLTTTRPDISFAVQQLSQFV 553
Query: 256 RKNAPPWGKEQTEAIKFLKKA 276
K + +++LK A
Sbjct: 554 SKPQQVHYQAAIRVLQYLKTA 616
>AL370071 similar to GP|15795135|dbj transposase-like protein {Arabidopsis
thaliana}, partial (3%)
Length = 370
Score = 30.4 bits (67), Expect = 1.3
Identities = 15/50 (30%), Positives = 27/50 (54%), Gaps = 5/50 (10%)
Frame = +3
Query: 319 HASGQFKDAELHYHTTFKETLAVKYGIKRFDFHLRGHH-----FEVQIDN 363
HA+G F +A+ HY F++ + VK G++ + H E+Q+D+
Sbjct: 105 HAAGYFLNAQYHYSPGFRDDVKVKRGLQHCITRMVTDHEERSKIEIQLDD 254
>BQ141686 homologue to PIR|S34666|S346 glycine-rich protein - common tobacco,
partial (6%)
Length = 1132
Score = 29.6 bits (65), Expect = 2.3
Identities = 27/113 (23%), Positives = 46/113 (39%), Gaps = 3/113 (2%)
Frame = +3
Query: 260 PPWG-KEQTEAIKFLKKAAQNPPPLKIPGDGKRILQTDASDHYWAAVLIEEIDGQKLYCG 318
PP G EQ + LK+AAQ + P +GKR+++ W + G++ CG
Sbjct: 144 PPTGDSEQMGPRRLLKRAAQKRT--RGPNNGKRVIKAGWGGGRWGVGVGGRK*GRRAACG 317
Query: 319 HASGQFKD--AELHYHTTFKETLAVKYGIKRFDFHLRGHHFEVQIDNSSFPKM 369
A G D + + +T G ++ G E+ + + +P M
Sbjct: 318 VAKGSSGDISKNITFRVRVPKTNRNNRGTQKNTGIGPGRKIELNLRSRQYPNM 476
>AL374846 weakly similar to PIR|T05449|T054 hypothetical protein F7K2.130 -
Arabidopsis thaliana, partial (11%)
Length = 511
Score = 29.3 bits (64), Expect = 2.9
Identities = 17/38 (44%), Positives = 23/38 (59%)
Frame = -2
Query: 326 DAELHYHTTFKETLAVKYGIKRFDFHLRGHHFEVQIDN 363
++E TF +L+V + IKRFDF LR H F V +N
Sbjct: 297 ESERQCGKTFATSLSVGFQIKRFDF-LR*HKFNVLHEN 187
>TC83085 weakly similar to GP|11994101|dbj|BAB01104.
gb|AAD39678.1~gene_id:MYF24.10~similar to unknown
protein {Arabidopsis thaliana}, partial (41%)
Length = 1186
Score = 29.3 bits (64), Expect = 2.9
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = -3
Query: 160 ESHKKFLQQFYDICSKHGVMLSSRKSQIATKEVDFLGMHF 199
E H K L F +KH + LS + + KE+ LG++F
Sbjct: 611 EHHSKLLDLFSPGSTKHSLQLSGWNTLCSRKELSVLGLNF 492
>BF004404 similar to GP|21928159|gb AT4g02010/T10M13_2 {Arabidopsis
thaliana}, partial (26%)
Length = 592
Score = 28.9 bits (63), Expect = 3.9
Identities = 16/51 (31%), Positives = 24/51 (46%), Gaps = 1/51 (1%)
Frame = -3
Query: 166 LQQFYDICSKHGVMLSSR-KSQIATKEVDFLGMHFTEGKFVPQPHIVEELP 215
L +C HG S R +S A F+G+ ++G+F+P H E P
Sbjct: 431 LNAIKSLCITHGCESSCRYESPRAASRAIFIGVSQSKGQFIPMGHYHEAKP 279
>BG457911 GP|3047314|gb| ubiquitin fusion protein {Magnaporthe grisea},
complete
Length = 537
Score = 28.5 bits (62), Expect = 5.0
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +1
Query: 87 DMKSGFWQLGLDPKDRPKTAFCIPN 111
D+ SG W LG+D KDR + F N
Sbjct: 403 DVASGIWHLGVDGKDRGELFFFCSN 477
>TC82766 similar to GP|3128183|gb|AAC16087.1| unknown protein {Arabidopsis
thaliana}, partial (10%)
Length = 690
Score = 27.7 bits (60), Expect = 8.6
Identities = 18/61 (29%), Positives = 34/61 (55%), Gaps = 7/61 (11%)
Frame = +2
Query: 264 KEQTEAIKFLKKAAQNPP------PLKIPG-DGKRILQTDASDHYWAAVLIEEIDGQKLY 316
+EQ + I+ + KA QN P++I DG+++ +TD + + + +EE++G L
Sbjct: 287 EEQMKRIEAIFKAKQNQKWQQCHNPIQITNFDGEQLTKTDNVEDDESWMTMEEVEGATLE 466
Query: 317 C 317
C
Sbjct: 467 C 469
>TC80484 similar to GP|11994729|dbj|BAB03045.
gene_id:F5N5.17~pir||G71408~similar to unknown protein
{Arabidopsis thaliana}, partial (55%)
Length = 1202
Score = 27.7 bits (60), Expect = 8.6
Identities = 21/68 (30%), Positives = 29/68 (41%), Gaps = 7/68 (10%)
Frame = +3
Query: 223 TIKQIQQFL-----GILNYIRDFIPHVSKYTSKLSKMLRKNAPPWGKEQTEAIKFLKKAA 277
T K I QFL G + +R + VS+ + K PPW K + K+L +
Sbjct: 534 TFKAILQFLPYIFVGKSDRLRQIVAVVSEAAKQSLKKKGMPVPPWRKSEYMLAKWLSTSC 713
Query: 278 Q--NPPPL 283
NP PL
Sbjct: 714 TRVNPSPL 737
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.321 0.138 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,883,454
Number of Sequences: 36976
Number of extensions: 209143
Number of successful extensions: 1042
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 1029
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1040
length of query: 418
length of database: 9,014,727
effective HSP length: 99
effective length of query: 319
effective length of database: 5,354,103
effective search space: 1707958857
effective search space used: 1707958857
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0490.11