BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0400a.7
(382 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC83671 similar to PIR|T09416|T09416 coil protein PO22 microspo... 310 6e-85
BG587016 similar to PIR|T09416|T09 coil protein PO22 microspore... 277 4e-80
TC76859 Enod8.1 [Medicago truncatula]; early nodule-specific pro... 289 1e-78
BI270789 similar to PIR|T09416|T09 coil protein PO22 microspore... 248 8e-72
TC93652 Enod8.2 [Medicago truncatula] 183 2e-71
TC82515 similar to GP|3688284|emb|CAA09694.1 lanatoside 15'-O-ac... 242 2e-64
NP429751 NP429751|AF463407.1|AAL68830.1 Enod8.3 [Medicago trunca... 239 1e-63
TC90156 similar to PIR|B86227|B86227 hypothetical protein [impor... 146 2e-59
TC90006 similar to PIR|A96590|A96590 hypothetical protein T22H22... 201 4e-52
TC81963 Enod8-like protein [Medicago truncatula] 201 4e-52
TC83147 similar to PIR|T48618|T48618 early nodule-specific prote... 174 4e-44
TC84309 similar to GP|9294302|dbj|BAB02204.1 nodulin-like protei... 157 6e-39
AJ497919 similar to PIR|T09416|T094 coil protein PO22 microspor... 142 2e-34
TC89608 similar to PIR|T01143|T01143 probable GDSL-motif lipase/... 117 6e-27
TC86678 similar to PIR|E96579|E96579 hypothetical protein T18A20... 117 6e-27
TC83609 similar to GP|5295941|dbj|BAA81842.1 ESTs AU075322(C1110... 106 1e-23
TC79343 weakly similar to PIR|E86411|E86411 protein F1K23.18 [im... 105 2e-23
TC90972 similar to GP|10638955|emb|CAB81548. putative proline-ri... 105 3e-23
TC88477 similar to GP|21592417|gb|AAM64368.1 lipase/hydrolase p... 104 6e-23
TC76727 similar to GP|10638955|emb|CAB81548. putative proline-ri... 103 1e-22
>TC83671 similar to PIR|T09416|T09416 coil protein PO22
microspore/pollen-specific - alfalfa, partial (60%)
Length = 671
Score = 310 bits (794), Expect = 6e-85
Identities = 154/231 (66%), Positives = 179/231 (76%)
Frame = +3
Query: 13 FALCVACTFIQIPSGNGSYSSSSKCSYPAVYNFGDSNSDTGVVYAAFAGLQSPGGISFFG 72
F V C FIQ+ SS KC YPA+YNFGDSNSDTG +YA + +Q P GI+FFG
Sbjct: 3 FRTRVTCKFIQV--------SSDKCVYPAIYNFGDSNSDTGTIYATYTSVQPPNGITFFG 158
Query: 73 NLSGRASDGRLIIDFITEELEIPYLSAYLNSIGSNYRHGANFAAGGASIRPVYGFSPFYL 132
N+SGRASDGRLIID ITEEL++PYLSAYLNS+GSNYRHGANFA GASIRP G+ F L
Sbjct: 159 NISGRASDGRLIIDSITEELKLPYLSAYLNSVGSNYRHGANFAVSGASIRP-RGYHLFNL 335
Query: 133 GMQVAQFIQLQSHIENLLNQFSSNRTEPPFKSYLPRPEDFSKALYTIDIGQNDLGFGLMH 192
G+QV+QFI +SH + L NQ S+NRTEP KS LPRPEDFSKALYTIDIGQNDL G +
Sbjct: 336 GLQVSQFILFKSHTKILFNQLSNNRTEPSLKSGLPRPEDFSKALYTIDIGQNDLAHGFQY 515
Query: 193 TSEEEVLRSIPEMMRNFTYDVQVLYDVGARVFWIHNTGPIGCLPTSSIFYE 243
TSEE+V RSIPE++ NF+ V+ LY+ GARVFWIHNTGPIGCLP + Y+
Sbjct: 516 TSEEQVQRSIPEILSNFSQSVKQLYNEGARVFWIHNTGPIGCLPFNYYTYK 668
>BG587016 similar to PIR|T09416|T09 coil protein PO22
microspore/pollen-specific - alfalfa, partial (55%)
Length = 696
Score = 277 bits (709), Expect(2) = 4e-80
Identities = 135/193 (69%), Positives = 158/193 (80%)
Frame = +3
Query: 36 KCSYPAVYNFGDSNSDTGVVYAAFAGLQSPGGISFFGNLSGRASDGRLIIDFITEELEIP 95
KC YPA+YNFGDSNSDTG A + + P GIS+FG+ +GRASDGRLIIDFI+EEL++P
Sbjct: 117 KCEYPAIYNFGDSNSDTGAANAIYTAVTPPNGISYFGSTTGRASDGRLIIDFISEELKLP 296
Query: 96 YLSAYLNSIGSNYRHGANFAAGGASIRPVYGFSPFYLGMQVAQFIQLQSHIENLLNQFSS 155
YLSAYLNSIGSNYRHGANFA GGASIRP G+SP +LG+QV+QFI +SH + L NQ S
Sbjct: 297 YLSAYLNSIGSNYRHGANFAVGGASIRP-GGYSPIFLGLQVSQFILFKSHTKILFNQLSD 473
Query: 156 NRTEPPFKSYLPRPEDFSKALYTIDIGQNDLGFGLMHTSEEEVLRSIPEMMRNFTYDVQV 215
NRTE PFKS LPR E+FSKALYTIDIGQNDL GL +TSEE+V RSIP+++ F+ VQ
Sbjct: 474 NRTESPFKSGLPRNEEFSKALYTIDIGQNDLAIGLQNTSEEQVKRSIPDILSQFSQAVQQ 653
Query: 216 LYDVGARVFWIHN 228
LY+ GARVFWIHN
Sbjct: 654 LYNEGARVFWIHN 692
Score = 38.9 bits (89), Expect(2) = 4e-80
Identities = 18/28 (64%), Positives = 19/28 (67%)
Frame = +1
Query: 1 MNSRTLIHALWCFALCVACTFIQIPSGN 28
MNS LIH LW F LCV TFIQ+ S N
Sbjct: 19 MNSMRLIHVLWFFNLCVTFTFIQVLSEN 102
>TC76859 Enod8.1 [Medicago truncatula]; early nodule-specific protein
Length = 1350
Score = 289 bits (740), Expect = 1e-78
Identities = 153/359 (42%), Positives = 215/359 (59%), Gaps = 10/359 (2%)
Frame = +2
Query: 33 SSSKCSYPAVYNFGDSNSDTGVVYAAFAGLQSPGGISFFGNLSGRASDGRLIIDFITEEL 92
++ C +PA+++FG SN DTG + AAF SP G ++F +GR SDGR+I+DFI
Sbjct: 128 ATKNCDFPAIFSFGASNVDTGGLAAAFRAPPSPYGETYFHRSTGRFSDGRIILDFIARSF 307
Query: 93 EIPYLSAYLNSIGSNYRHGANFAAGGA------SIRPVYGFSPFYLGMQVAQFIQLQSHI 146
+PYLS YLNS+GSN+ HGANFA+GG+ SI P SPF L +Q QF + S
Sbjct: 308 RLPYLSPYLNSLGSNFTHGANFASGGSTINIPKSILPNGKLSPFSLQIQYIQFKEFISKT 487
Query: 147 ENLLNQFSSNRTEPPFKSYLPRPEDFSKALYTIDIGQNDLGFGLM-HTSEEEVLRSIPEM 205
+ + +Q F + +P+ + FSKALY DIGQNDL G + + ++V ++P++
Sbjct: 488 KLIRDQGG------VFATLIPKEDYFSKALYIFDIGQNDLTIGFFGNKTIQQVNATVPDI 649
Query: 206 MRNFTYDVQVLYDVGARVFWIHNTGPIGCLPTSSIFYEPKKGNLDANGCVIPHNKIAQEF 265
+ N+ +++ +Y++GAR FWIH TGP GC P I D+ GC +N+++Q F
Sbjct: 650 VNNYIENIKNIYNLGARSFWIHGTGPKGCAPV--ILANFPSAIKDSYGCAKQYNEVSQYF 823
Query: 266 NRQLKDQVFQLRRNLPKAKFTYVDVYTAKYELISNASKQGFVNPLEVCC--GSYYGYRID 323
N +LK+ + +LR NL A TYVD+YT KY L +N K GF P CC G Y +
Sbjct: 824 NFKLKEALAELRSNLSSAAITYVDIYTPKYSLFTNPEKYGFELPFVACCGYGGEYNIGVG 1003
Query: 324 CGKKAVVNGT-VYGNPCKNPSQHISWDGVHYTQAANKWVAKHIRDGSLSDPPVPIGQAC 381
CG +NGT + CKNPS I WDGVHYT+AAN+ V I G +DPP+ + +AC
Sbjct: 1004CGASININGTKIVAGSCKNPSTRIIWDGVHYTEAANEIVFSQILTGVFNDPPISLDRAC 1180
>BI270789 similar to PIR|T09416|T09 coil protein PO22
microspore/pollen-specific - alfalfa, partial (62%)
Length = 657
Score = 248 bits (634), Expect(2) = 8e-72
Identities = 129/199 (64%), Positives = 149/199 (74%)
Frame = +1
Query: 53 GVVYAAFAGLQSPGGISFFGNLSGRASDGRLIIDFITEELEIPYLSAYLNSIGSNYRHGA 112
G YA F Q P GISF GN+SGRASDGRLIID+ITEEL++PYLSAYLNS+GSNYR+GA
Sbjct: 76 GTAYATFLCNQPPNGISF-GNISGRASDGRLIIDYITEELKVPYLSAYLNSVGSNYRYGA 252
Query: 113 NFAAGGASIRPVYGFSPFYLGMQVAQFIQLQSHIENLLNQFSSNRTEPPFKSYLPRPEDF 172
NFAAGGASIRP GFSPF+LG+QV QFIQ +SH L N N TEP KS LPRPEDF
Sbjct: 253 NFAAGGASIRPGSGFSPFHLGLQVDQFIQFKSHTRILFN----NGTEPSLKSGLPRPEDF 420
Query: 173 SKALYTIDIGQNDLGFGLMHTSEEEVLRSIPEMMRNFTYDVQVLYDVGARVFWIHNTGPI 232
ALYTIDIG NDL G +H SEE+V S PE++ +F+ V+ LY+V ARVFWIHN GP+
Sbjct: 421 CTALYTIDIGLNDLASGFLHASEEQVQMSFPEILGHFSKAVKQLYNVXARVFWIHNVGPV 600
Query: 233 GCLPTSSIFYEPKKGNLDA 251
GC P+ KKG L+A
Sbjct: 601 GCCPSIITPNXNKKGILNA 657
Score = 40.0 bits (92), Expect(2) = 8e-72
Identities = 16/20 (80%), Positives = 18/20 (90%)
Frame = +3
Query: 33 SSSKCSYPAVYNFGDSNSDT 52
SS +C YPA+YNFGDSNSDT
Sbjct: 15 SSHECVYPAIYNFGDSNSDT 74
>TC93652 Enod8.2 [Medicago truncatula]
Length = 1195
Score = 183 bits (464), Expect(2) = 2e-71
Identities = 102/259 (39%), Positives = 146/259 (55%), Gaps = 4/259 (1%)
Frame = +2
Query: 127 FSPFYLGMQVAQFIQLQSHIENLLNQFSSNRTEPPFKSYLPRPEDFSKALYTIDIGQNDL 186
FSPF L +Q ++ NL+ F + +P+ + FSKALYT DIGQNDL
Sbjct: 419 FSPFSLQIQGTFNSKIFISKTNLIRDQGG-----VFATLIPKEDYFSKALYTFDIGQNDL 583
Query: 187 GFGLM-HTSEEEVLRSIPEMMRNFTYDVQVLYDVGARVFWIHNTGPIGCLPTSSIFYEPK 245
G + + ++V ++P+++ NF +++ +Y++GAR FWIH+T P GC PT I
Sbjct: 584 IGGYFGNKTIKQVNATVPDIVNNFIVNIKNIYNLGARSFWIHSTVPSGCTPT--ILANFP 757
Query: 246 KGNLDANGCVIPHNKIAQEFNRQLKDQVFQLRRNLPKAKFTYVDVYTAKYELISNASKQG 305
D+ GC +N+++Q FN +LK + QLR +LP A TYVD+Y+ Y L N K G
Sbjct: 758 SAIKDSYGCAKQYNEVSQYFNLKLKKALAQLRVDLPLAAITYVDIYSPNYSLFQNPKKYG 937
Query: 306 FVNPLEVCC--GSYYGYRIDCGKKAVVNGT-VYGNPCKNPSQHISWDGVHYTQAANKWVA 362
F P CC G Y R+ CG+ +NGT + CKNPS I WDG H+T+ K V
Sbjct: 938 FELPHVACCGYGGKYNIRVGCGETLNINGTKIEAGSCKNPSTRIIWDGSHFTERRYKIVF 1117
Query: 363 KHIRDGSLSDPPVPIGQAC 381
I G+ SDPP+ + +AC
Sbjct: 1118DQISTGAFSDPPISLNRAC 1174
Score = 104 bits (259), Expect(2) = 2e-71
Identities = 47/89 (52%), Positives = 65/89 (72%)
Frame = +3
Query: 33 SSSKCSYPAVYNFGDSNSDTGVVYAAFAGLQSPGGISFFGNLSGRASDGRLIIDFITEEL 92
++ C +PA+++FG SN DTG + AAF SP G ++F +GR SDGR+I+DFI +
Sbjct: 117 ATRNCDFPAIFSFGASNVDTGGLAAAFQAPPSPYGETYFHRSTGRFSDGRIILDFIAQSF 296
Query: 93 EIPYLSAYLNSIGSNYRHGANFAAGGASI 121
+PYLS YLNS+GSN+ HGANFA GG++I
Sbjct: 297 GLPYLSPYLNSLGSNFTHGANFATGGSTI 383
>TC82515 similar to GP|3688284|emb|CAA09694.1 lanatoside
15'-O-acetylesterase {Digitalis lanata}, partial (76%)
Length = 1230
Score = 242 bits (618), Expect = 2e-64
Identities = 133/359 (37%), Positives = 204/359 (56%), Gaps = 9/359 (2%)
Frame = +1
Query: 32 SSSSKCSYPAVYNFGDSNSDTGVVYAAFAGLQSPGGISFFGNLSGRASDGRLIIDFITEE 91
SS SKC + ++NFGDSNSDTG Y+AF P G+++F GR+SDGRLI+DF+ E
Sbjct: 118 SSYSKCDFQGIFNFGDSNSDTGGFYSAFPAQPIPYGMTYFKTPVGRSSDGRLIVDFLAEA 297
Query: 92 LEIPYLSAYLNSIGSNYRHGANFAAGGASIRP------VYGFSPFYLGMQVAQFIQLQSH 145
L +PYLS YL SIGS+Y HGANFA +++ V G SPF L +Q+ Q Q ++
Sbjct: 298 LGLPYLSPYLQSIGSDYTHGANFATSASTVLLPTTSLFVSGLSPFALQIQLRQMQQFRAK 477
Query: 146 IENLLNQFSSNRTEPPFKSYLPRPEDFSKALYTIDIGQNDLGFGLMHTSEEEVLRS-IPE 204
+ + + + K +P P+ F K++Y IGQND + + L++ +P+
Sbjct: 478 VHDFHKRDPLKPSTCASKIKIPSPDIFGKSIYMFYIGQNDFTSKIAASGGINGLKNYLPQ 657
Query: 205 MMRNFTYDVQVLYDV-GARVFWIHNTGPIGCLPTSSIFYEPKKGNLDANGCVIPHNKIAQ 263
++ ++ LY G R F + N GP+GC P + +L+ +GC+I +N
Sbjct: 658 IIYQIASAIKELYYAQGGRTFMVLNLGPVGCYPGYLVELPHTSSDLNEHGCIITYNNAVD 837
Query: 264 EFNRQLKDQVFQLRRNLPKAKFTYVDVYTAKYELISNASKQGFVNPLEVCCGSYYG-YRI 322
++N+ LK+ + Q R++L A YVD +A EL + + G + + CCG G Y
Sbjct: 838 DYNKLLKETLTQTRKSLSDASLIYVDTNSALMELFRHPTSYGLKHSTKACCGHGGGDYNF 1017
Query: 323 DCGKKAVVNGTVYGNPCKNPSQHISWDGVHYTQAANKWVAKHIRDGSLSDPPVPIGQAC 381
D KA+ G + + C++P ++SWDG+H+T+AANK +A I +GSLSDPP + + C
Sbjct: 1018D--PKALC-GNMLASACEDPQNYVSWDGIHFTEAANKIIAMAILNGSLSDPPFLLHKLC 1185
>NP429751 NP429751|AF463407.1|AAL68830.1 Enod8.3 [Medicago truncatula]
Length = 902
Score = 239 bits (611), Expect = 1e-63
Identities = 130/301 (43%), Positives = 184/301 (60%), Gaps = 13/301 (4%)
Frame = +3
Query: 94 IPYLSAYLNSIGSNYRHGANFAAGGASIR------PVYGFSPFYLGMQVAQFIQLQSHIE 147
+PYLS YLNS+GSN+ HGANFA G++I+ P FSPF L +Q QF +
Sbjct: 15 LPYLSPYLNSLGSNFTHGANFATAGSTIKIPNSIIPNGMFSPFSLQIQSIQFKDFIPKAK 194
Query: 148 NLLNQFSSNRTEPPFKSYLPRPEDFSKALYTIDIGQNDLGFGLM-HTSEEEVLRSIPEMM 206
+ +Q F + +P+ + +SKALYT DIGQNDL G + + ++V ++P+++
Sbjct: 195 FIRDQGGV------FATLIPKEDYYSKALYTFDIGQNDLTAGFFGNKTIQQVNTTVPDIV 356
Query: 207 RNFTYDVQVLYDVGARVFWIHNTGPIGCLPTSSIFYEPKKGNLDANGCVIPHNKIAQEFN 266
++F +++ +Y++GAR FWIHNTGPIGC+P I D GC +N+++Q FN
Sbjct: 357 KSFIDNIKNIYNLGARSFWIHNTGPIGCVPL--ILANFPSAIKDRYGCAKQYNEVSQYFN 530
Query: 267 RQLKDQVFQLRRNLPKAKFTYVDVYTAKYELISNASKQGFVNPLEVCCGS----YYGYRI 322
+LK+ + QLR++LP A TYVD+Y+ KY L N K GF PL CCG+ Y R
Sbjct: 531 LKLKEALAQLRKDLPLAAITYVDIYSPKYSLFQNPKKYGFELPLVACCGNGGKYNYNIRA 710
Query: 323 DCGKKAVVNG--TVYGNPCKNPSQHISWDGVHYTQAANKWVAKHIRDGSLSDPPVPIGQA 380
CG +NG TV G+ CK PS I WDG HYT+AANK V I +G+ +DPP+P+ +A
Sbjct: 711 GCGATININGTNTVVGS-CKKPSTRIIWDGTHYTEAANKIVFDQISNGAFTDPPIPLNRA 887
Query: 381 C 381
C
Sbjct: 888 C 890
>TC90156 similar to PIR|B86227|B86227 hypothetical protein [imported] -
Arabidopsis thaliana, partial (84%)
Length = 1421
Score = 146 bits (369), Expect(2) = 2e-59
Identities = 75/190 (39%), Positives = 105/190 (54%), Gaps = 5/190 (2%)
Frame = +1
Query: 197 EVLRSIPEMMRNFTYDVQVLYDVGARVFWIHNTGPIGCLPTSSIFYEPKKGNLDANGCVI 256
+V++ IP ++ V+ LY+ G R FW+HNTGP GCLP + K +LD+ GC+
Sbjct: 586 QVIKRIPTVITEIENAVKSLYNEGGRKFWVHNTGPFGCLP--KLIALSXKKDLDSFGCLS 759
Query: 257 PHNKIAQEFNRQLKDQVFQLRRNLPKAKFTYVDVYTAKYELISNASKQGFVNPLEVCCG- 315
+N A+ FN L +LR L A YVD+Y K +LI+NA+K GF NPL VCCG
Sbjct: 760 SYNSAARLFNEALYHSSQKLRTELKDATLVYVDIYAIKNDLITNATKYGFTNPLMVCCGF 939
Query: 316 ----SYYGYRIDCGKKAVVNGTVYGNPCKNPSQHISWDGVHYTQAANKWVAKHIRDGSLS 371
+ R+ CG+ C S+++SWDG+HYT+AAN W+A I + S
Sbjct: 940 GGPPYNFDARVTCGQPGY-------QVCDEGSRYVSWDGIHYTEAANTWIASKILSTAYS 1098
Query: 372 DPPVPIGQAC 381
P +P G C
Sbjct: 1099TPRIPFGFFC 1128
Score = 100 bits (250), Expect(2) = 2e-59
Identities = 67/170 (39%), Positives = 89/170 (51%), Gaps = 2/170 (1%)
Frame = +3
Query: 19 CTFIQIPSGNGSYSSSSKCSYPAVYNFGDSNSDTGVVYAAFA-GLQSPGGISFFGNLSGR 77
C F S S SS V+ FGDSNSDTG + + + P G +FF +GR
Sbjct: 75 CIFFSSVSLALSVSSGCSSKPAVVFVFGDSNSDTGGLVSGLGFPVNLPNGRTFFHRSTGR 254
Query: 78 ASDGRLIIDFITEELEIPYLSAYLNSI-GSNYRHGANFAAGGASIRPVYGFSPFYLGMQV 136
SDGRL+IDF+ + L +L+ YL+S+ GS + +GANFA G+S P Y PF L +QV
Sbjct: 255 LSDGRLVIDFLCQSLNTRFLTPYLDSMSGSTFTNGANFAVVGSSTLPKY--LPFSLNIQV 428
Query: 137 AQFIQLQSHIENLLNQFSSNRTEPPFKSYLPRPEDFSKALYTIDIGQNDL 186
QF ++ L + N + + F ALY IDIGQNDL
Sbjct: 429 MQFQHFKARSLQLATSGAKN---------MINDQGFRDALYLIDIGQNDL 551
>TC90006 similar to PIR|A96590|A96590 hypothetical protein T22H22.20
[imported] - Arabidopsis thaliana, partial (63%)
Length = 858
Score = 201 bits (511), Expect = 4e-52
Identities = 114/274 (41%), Positives = 159/274 (57%), Gaps = 4/274 (1%)
Frame = +3
Query: 15 LCVACTFIQIPSGNGSYSSSSKCSYPAVYNFGDSNSDTG-VVYAAFAGLQSPGGISFFGN 73
+C+ T +P + S +PAV+NFGDSNSDTG +V A F L P G ++F
Sbjct: 60 ICIITTIFLLPC-----AKSIHLDFPAVFNFGDSNSDTGTLVTAGFESLYPPNGHTYFHL 224
Query: 74 LSGRASDGRLIIDFITEELEIPYLSAYLNSIG-SNYRHGANFAAGGASIRPVYGFS--PF 130
SGR SDGRLIIDF+ + L++P+L+AYL+S+G N+R G NFAA G++I P S PF
Sbjct: 225 PSGRYSDGRLIIDFLMDALDLPFLNAYLDSLGLPNFRKGCNFAAAGSTILPATASSICPF 404
Query: 131 YLGMQVAQFIQLQSHIENLLNQFSSNRTEPPFKSYLPRPEDFSKALYTIDIGQNDLGFGL 190
G+QV+QF++ ++ LL+ F Y+P + F K LY DIGQNDL
Sbjct: 405 SFGIQVSQFLKFKARALELLSGKGRK-----FDKYVPSEDIFEKGLYMFDIGQNDLAGAF 569
Query: 191 MHTSEEEVLRSIPEMMRNFTYDVQVLYDVGARVFWIHNTGPIGCLPTSSIFYEPKKGNLD 250
+ ++VL SIP ++ F ++ LYD GAR FWIHNTGP+GCL + + LD
Sbjct: 570 YSKTLDQVLASIPTILLEFESGIKRLYDEGARYFWIHNTGPLGCLAQNVAKFGTDPSKLD 749
Query: 251 ANGCVIPHNKIAQEFNRQLKDQVFQLRRNLPKAK 284
GCV HN+ + FN QL +L ++ + K
Sbjct: 750 ELGCVSGHNQAVKTFNLQLHALCSKLAGSISRFK 851
>TC81963 Enod8-like protein [Medicago truncatula]
Length = 783
Score = 201 bits (511), Expect = 4e-52
Identities = 103/212 (48%), Positives = 144/212 (67%), Gaps = 7/212 (3%)
Frame = +3
Query: 32 SSSSKCSYPAVYNFGDSNSDTGVVYAAFAGLQSPGGISFFGNLSGRASDGRLIIDFITEE 91
SS+S+C++ A++NFGDSNSDTG + A+F + P G ++F +GR SDGRLI+DFI +
Sbjct: 162 SSNSECNFRAIFNFGDSNSDTGGLAASFVAPKPPYGETYFHRPNGRFSDGRLIVDFIAQS 341
Query: 92 LEIPYLSAYLNSIGSNYRHGANFAAGGASIR------PVYGFSPFYLGMQVAQFIQLQSH 145
+PYLSAYL+S+G+N+ HGANFA ++IR P GFSPFYL +Q QF +
Sbjct: 342 FGLPYLSAYLDSLGTNFSHGANFATTSSTIRPPPSIIPQGGFSPFYLDVQYTQFRDFKPR 521
Query: 146 IENLLNQFSSNRTEPPFKSYLPRPEDFSKALYTIDIGQNDLGFGLM-HTSEEEVLRSIPE 204
+ + Q F S +P+ E FSKALYT DIGQNDLG G + + ++V S+PE
Sbjct: 522 TQFIRQQGGL------FASLMPKEEYFSKALYTFDIGQNDLGAGFFGNMTIQQVNASVPE 683
Query: 205 MMRNFTYDVQVLYDVGARVFWIHNTGPIGCLP 236
++ +F+ +V+ +Y++G R FWIHNTGPIGCLP
Sbjct: 684 IINSFSKNVKDIYNLGGRSFWIHNTGPIGCLP 779
>TC83147 similar to PIR|T48618|T48618 early nodule-specific protein-like -
Arabidopsis thaliana, partial (43%)
Length = 653
Score = 174 bits (442), Expect = 4e-44
Identities = 95/183 (51%), Positives = 121/183 (65%), Gaps = 6/183 (3%)
Frame = +1
Query: 13 FALCVACTFIQIPSGNGSYSSSSKCSYPAVYNFGDSNSDTGVVYAAFAGLQSPGGISFFG 72
F CV C ++ SS CS+PA+YNFGDSNSDTG + AAF + SP G FF
Sbjct: 34 FLSCVKCVELK--------DSSPSCSFPAIYNFGDSNSDTGGISAAFVPIPSPYGQGFFH 189
Query: 73 NLSGRASDGRLIIDFITEELEIPYLSAYLNSIGSNYRHGANFAAGGASIRP------VYG 126
GR SDGR+I+D+I ++L+ PYLSAYLNS+G+NYRHGANFA GG++IR YG
Sbjct: 190 KPFGRDSDGRVILDYIADKLKWPYLSAYLNSLGTNYRHGANFATGGSTIRKQNETIFQYG 369
Query: 127 FSPFYLGMQVAQFIQLQSHIENLLNQFSSNRTEPPFKSYLPRPEDFSKALYTIDIGQNDL 186
SPF L +Q+ QF Q ++ + L Q ++N E +S LP PE+F+KALYT DIGQNDL
Sbjct: 370 ISPFSLDIQIVQFNQFKARTKQLY-QEANNSLE---RSKLPVPEEFAKALYTFDIGQNDL 537
Query: 187 GFG 189
G
Sbjct: 538 SVG 546
>TC84309 similar to GP|9294302|dbj|BAB02204.1 nodulin-like protein protein
{Arabidopsis thaliana}, partial (47%)
Length = 672
Score = 157 bits (397), Expect = 6e-39
Identities = 87/208 (41%), Positives = 125/208 (59%), Gaps = 7/208 (3%)
Frame = +2
Query: 17 VACTFIQIPSGNGSYSSSSKCSYPAVYNFGDSNSDTGVVYAAFAGLQSPGGISFFGNLSG 76
V TF+ + + CS+PA++NFGDSNSDTG + AAF P GI+FF +G
Sbjct: 59 VISTFLVVVMVPSPVIGARNCSFPAIFNFGDSNSDTGGLSAAFGQAPPPNGITFFQTPAG 238
Query: 77 RASDGRLIIDFITEELEIPYLSAYLNSIGSNYRHGANFAAGGASIRP------VYGFSPF 130
R SDGRLIIDF+ + L +PYLSAYL+S+GSN+ +GANFA G++IRP G+SP
Sbjct: 239 RFSDGRLIIDFLAQNLSLPYLSAYLDSVGSNFSNGANFATAGSTIRPQNTTKSQSGYSPI 418
Query: 131 YLGMQVAQFIQLQSHIENLLNQFSSNRTEPPFKSYLPRPEDFSKALYTIDIGQNDLGFGL 190
L +Q+ Q+ ++ + F LP+ E FS+ALYT DIGQNDL G
Sbjct: 419 SLDVQLIQYSDFKA------RSILVRKKGGVFMKLLPKEEYFSEALYTFDIGQNDLTAGY 580
Query: 191 -MHTSEEEVLRSIPEMMRNFTYDVQVLY 217
++ + E+V IP+++ F+ ++ +Y
Sbjct: 581 KLNMTTEQVKAYIPDVLGQFSDVIRSVY 664
>AJ497919 similar to PIR|T09416|T094 coil protein PO22
microspore/pollen-specific - alfalfa, partial (9%)
Length = 549
Score = 142 bits (359), Expect = 2e-34
Identities = 59/77 (76%), Positives = 68/77 (87%)
Frame = +1
Query: 305 GFVNPLEVCCGSYYGYRIDCGKKAVVNGTVYGNPCKNPSQHISWDGVHYTQAANKWVAKH 364
GFVNPLE CCGSYYGY I+CG KA++NGTVYGNPC NPS+HISWDG+HY+QAAN+WVAK
Sbjct: 145 GFVNPLEFCCGSYYGYHINCGLKAIINGTVYGNPCDNPSKHISWDGIHYSQAANQWVAKQ 324
Query: 365 IRDGSLSDPPVPIGQAC 381
I GS SDPPV +G+AC
Sbjct: 325 ILYGSFSDPPVSVGKAC 375
>TC89608 similar to PIR|T01143|T01143 probable GDSL-motif lipase/hydrolase
[imported] - Arabidopsis thaliana, partial (70%)
Length = 959
Score = 117 bits (294), Expect = 6e-27
Identities = 82/281 (29%), Positives = 129/281 (45%), Gaps = 14/281 (4%)
Frame = +2
Query: 102 NSIGSNYRHGANFAAGGASIRPVYG---FSPFYLGMQVAQFIQLQSHIENLLNQFSSNRT 158
N+ G + +G N+A+GG I G + + +Q+ F + I+ LL Q +
Sbjct: 17 NATGKSILYGVNYASGGGGILNATGRIFVNRIGMDIQIDYFTITRKQIDKLLGQSKA--- 187
Query: 159 EPPFKSYLPRPEDFSKALYTIDIGQND---------LGFGLMHTSEEEVLRSIPEMMRNF 209
R K++++I +G ND L G + + + +M+ +F
Sbjct: 188 ---------RDFIMKKSIFSITVGANDFLNNYLLPVLSVGARISQSPDAF--VDDMINHF 334
Query: 210 TYDVQVLYDVGARVFWIHNTGPIGCLPTSSIFYEPKKGNLDANGCVIPHNKIAQEFNRQL 269
+ LY + AR F I N GPIGC+P Y+ L+ + CV NK+A ++N +L
Sbjct: 335 RGQLTRLYKMDARKFVIGNVGPIGCIP-----YQKTINQLNEDECVDLANKLAIQYNGRL 499
Query: 270 KDQVFQLRRNLPKAKFTYVDVYTAKYELISNASKQGFVNPLEVCCGS--YYGYRIDCGKK 327
KD + +L NLP A F +VY ELI N K GF CCG+ + I CG
Sbjct: 500 KDMLAELNDNLPGATFVLANVYDLVMELIKNYDKYGFTTSSRACCGNGGQFAGIIPCGPT 679
Query: 328 AVVNGTVYGNPCKNPSQHISWDGVHYTQAANKWVAKHIRDG 368
+ + C + +H+ WD H ++AAN +AK + DG
Sbjct: 680 SCI--------CNDRYKHVFWDPYHPSEAANIIIAKQLLDG 778
>TC86678 similar to PIR|E96579|E96579 hypothetical protein T18A20.15
[imported] - Arabidopsis thaliana, partial (43%)
Length = 1341
Score = 117 bits (294), Expect = 6e-27
Identities = 97/339 (28%), Positives = 151/339 (43%), Gaps = 14/339 (4%)
Frame = +2
Query: 38 SYPAVYNFGDSNSDTG----VVYAAFAGLQS-PGGISFFGNLSGRASDGRLIIDFITEEL 92
S A++ FGDS D G + F P G ++F +GR SDGRLI DFI E +
Sbjct: 173 SNKALFIFGDSFLDAGNNNYINTTTFDQANFLPYGETYFNFPTGRFSDGRLISDFIAEYV 352
Query: 93 EIPYLSAYLNSIGSNYRHGANFAAGGA-SIRPVYGFSPFYLGMQVAQFIQLQSHIENLLN 151
IP + +L + Y +G NFA+GGA ++ + S Q F ++ + + + L
Sbjct: 353 NIPLVPPFLQPDNNKYYNGVNFASGGAGALVETFQGSVIPFKTQAINFKKVTTWLRHKLG 532
Query: 152 QFSSNRTEPPFKSYLPRPEDFSKALYTIDIGQNDL-------GFGLMHTSEEEVLRSIPE 204
S S A+Y IG ND L H S E + +
Sbjct: 533 SSDSKTL-------------LSNAVYMFSIGSNDYLSPFLTNSDVLKHYSHTEYVAMV-- 667
Query: 205 MMRNFTYDVQVLYDVGARVFWIHNTGPIGCLPTSSIFYEPKKGNLDANGCVIPHNKIAQE 264
+ NFT ++ ++ GA+ F I N P+GCLP + I KG+ C+ + +A
Sbjct: 668 -IGNFTSTIKEIHKRGAKKFVILNLPPLGCLPGTRIIQSQGKGS-----CLEELSSLASI 829
Query: 265 FNRQLKDQVFQLRRNLPKAKFTYVDVYTAKYELISNASKQGFVNPLEVCCGS-YYGYRID 323
N+ L + + +L++ L KF+ D + +I++ K GF CCGS +
Sbjct: 830 HNQALYEVLLELQKQLRGFKFSLYDFNSDLSHMINHPLKYGFKEGKSACCGSGPFRGEYS 1009
Query: 324 CGKKAVVNGTVYGNPCKNPSQHISWDGVHYTQAANKWVA 362
CG K G + C P++ + WD H T++A K +A
Sbjct: 1010CGGK---RGEKHFELCDKPNESVFWDSYHLTESAYKQLA 1117
>TC83609 similar to GP|5295941|dbj|BAA81842.1 ESTs AU075322(C11109)
D22430(C11109) correspond to a region of the predicted
gene.~Similar to, partial (22%)
Length = 437
Score = 106 bits (265), Expect = 1e-23
Identities = 48/83 (57%), Positives = 62/83 (73%)
Frame = +2
Query: 33 SSSKCSYPAVYNFGDSNSDTGVVYAAFAGLQSPGGISFFGNLSGRASDGRLIIDFITEEL 92
S +C + A++NFGDSNSDTG YAAF P G+++F +GRASDGRL+IDFI + +
Sbjct: 179 SQGECDFKAIFNFGDSNSDTGGFYAAFPAESGPYGMTYFNKPAGRASDGRLVIDFIAQAI 358
Query: 93 EIPYLSAYLNSIGSNYRHGANFA 115
IP+LS YL SIGS Y+HGAN+A
Sbjct: 359 GIPFLSPYLQSIGSYYKHGANYA 427
>TC79343 weakly similar to PIR|E86411|E86411 protein F1K23.18 [imported] -
Arabidopsis thaliana, partial (52%)
Length = 1420
Score = 105 bits (263), Expect = 2e-23
Identities = 72/210 (34%), Positives = 105/210 (49%), Gaps = 6/210 (2%)
Frame = +3
Query: 170 EDFSKALYTI-DIGQNDLGFGL-MHTSEEEVLRSIPEMMRNFTYDVQVLYDVGARVFWIH 227
E F+ +L+ + +IG ND + L + S E+ +P ++ T + L D+GAR I
Sbjct: 594 EVFANSLFLMGEIGGNDFNYPLFIRRSIVEIKTYVPHVISAITSAINELIDLGARTLMIP 773
Query: 228 NTGPIGCLPTSSIFYEPK-KGNLDANGCVIPHNKIAQEFNRQLKDQVFQLRRNLPKAKFT 286
P+GC YE K D+ GC+ N+ A+ +N++L+ ++ +LRR P A
Sbjct: 774 GNFPLGCNVIYLTKYETTDKSQYDSAGCLKWLNEFAEFYNQELQYELHRLRRIHPHATII 953
Query: 287 YVDVYTAKYELISNASKQGFVNPLEVCCGSYYGYRI---DCGKKAVVNGTVYGNPCKNPS 343
Y D Y A L N +K GF L+ CCG Y CGK V C +PS
Sbjct: 954 YADYYNALLPLYQNPTKFGFTG-LKNCCGMGGSYNFGSGSCGKPGVF-------ACDDPS 1109
Query: 344 QHISWDGVHYTQAANKWVAKHIRDGSLSDP 373
Q+I WDGVH T+AA + +A I +G S P
Sbjct: 1110QYIGWDGVHLTEAAYRLIADGIINGPCSVP 1199
Score = 65.5 bits (158), Expect = 3e-11
Identities = 42/100 (42%), Positives = 58/100 (58%), Gaps = 10/100 (10%)
Frame = +2
Query: 38 SYPAVYNFGDSNSDTGVVYAAFAGLQS-----PGGISFFGNLSGRASDGRLIIDFITEEL 92
SY ++++FGDS +DTG +Y + P G ++F + SGR SDGRLIIDFI E L
Sbjct: 188 SYSSIFSFGDSIADTGNLYLSSQPPSDHCFFPPYGQTYFHHPSGRCSDGRLIIDFIAESL 367
Query: 93 EIPYLSAYL---NSI--GSNYRHGANFAAGGASIRPVYGF 127
IP + YL N + ++ + GANFA GA+ V F
Sbjct: 368 GIPMVKPYLGIKNGVLEDNSAKEGANFAVIGATALDVSFF 487
>TC90972 similar to GP|10638955|emb|CAB81548. putative proline-rich protein
APG isolog {Cicer arietinum}, partial (88%)
Length = 1258
Score = 105 bits (262), Expect = 3e-23
Identities = 94/341 (27%), Positives = 149/341 (43%), Gaps = 18/341 (5%)
Frame = +3
Query: 40 PAVYNFGDSNSDTG---VVYAAFAGLQSPGGISFFGNL-SGRASDGRLIIDFITEELEIP 95
PA+ FGDS D G ++ F P G F + +GR +G+L D E L
Sbjct: 105 PAIVTFGDSAVDVGNNDYLFTLFKANYPPYGRDFVXHKPTGRFCNGKLATDITAETLGFK 284
Query: 96 -YLSAYLN--SIGSNYRHGANFA--AGGASIRPVYGFSPFYLGMQVAQFIQLQSHIENLL 150
Y AYL+ + G N GANFA A G + L Q+ + + QS + +
Sbjct: 285 SYAPAYLSPQATGKNLLIGANFASAASGYDEKAAILNHAIPLSQQLKYYKEYQSKLSKIA 464
Query: 151 NQFSSNRTEPPFKSYLPRPEDFSKALYTIDIGQNDLGFGLMHTSEEEVLRSI--PEMMRN 208
S + K ALY + G +D + + ++ + P+
Sbjct: 465 G---SKKAASIIKG----------ALYLLSGGSSDF---IQNYYVNPLINKVVTPDQYSA 596
Query: 209 FTYD-----VQVLYDVGARVFWIHNTGPIGCLPTSSIFYEPKKGNLDANGCVIPHNKIAQ 263
+ D V+ LY +GAR + + P+GCLP + + GCV N AQ
Sbjct: 597 YLVDTYSSFVKDLYKLGARKIGVTSLPPLGCLPATRTLF-----GFHEKGCVTRINNDAQ 761
Query: 264 EFNRQLKDQVFQLRRNLPKAKFTYVDVYTAKYELISNASKQGFVNPLEVCCGS--YYGYR 321
FN+++ +L++ LP K ++Y YEL+ + SK GF + CCG+
Sbjct: 762 GFNKKINSATVKLQKQLPGLKIVVFNIYKPLYELVQSPSKFGFAEARKGCCGTGIVETTS 941
Query: 322 IDCGKKAVVNGTVYGNPCKNPSQHISWDGVHYTQAANKWVA 362
+ C +K++ GT C N +Q++ WD VH ++AAN+ +A
Sbjct: 942 LLCNQKSL--GT-----CSNATQYVFWDSVHPSEAANQILA 1043
>TC88477 similar to GP|21592417|gb|AAM64368.1 lipase/hydrolase putative
{Arabidopsis thaliana}, partial (92%)
Length = 1415
Score = 104 bits (259), Expect = 6e-23
Identities = 106/360 (29%), Positives = 153/360 (42%), Gaps = 22/360 (6%)
Frame = +2
Query: 40 PAVYNFGDSNSDTGVVYAAFAGLQS-------PGGISFFGNLSGRASDGRLIIDFITEEL 92
P + FGDS D G GLQS P GI F G +GR S+G+ +D I E L
Sbjct: 179 PWYFIFGDSLVDNG----NNNGLQSLARADYLPYGIDF-GGPTGRFSNGKTTVDAIAELL 343
Query: 93 EIP-YLSAYLNSIGSNYRHGANFAAGGASIRPVYGFSPFYLGMQVAQFIQLQSHIENLLN 151
Y+ Y ++ G N+A+ A IR G LG +++ Q+Q++ +
Sbjct: 344 GFDDYIPPYASASDDAILKGVNYASAAAGIREETGRQ---LGARLSFSAQVQNYQSTVSQ 514
Query: 152 QFSSNRTEPPFKSYLPRPEDFSKALYTIDIGQND-LGFGLM---------HTSEEEVLRS 201
+ TE S+L SK +Y+I +G ND L M +T +E
Sbjct: 515 VVNILGTEDQAASHL------SKCIYSIGLGSNDYLNNYFMPQFYNTHDQYTPDEYA--- 667
Query: 202 IPEMMRNFTYDVQVLYDVGARVFWIHNTGPIGCLPTSSIFYEPKKGNLDANGCVIPHNKI 261
++++++T ++ LY+ AR + G IGC P E + D CV N
Sbjct: 668 -DDLIQSYTEQLRTLYNN*ARKMVLFGIGQIGCSPN-----ELATRSADGVTCVEEINSA 829
Query: 262 AQEFNRQLKDQVFQLRRNLPKAKFTYVDVYTAKYELISNASKQGFVNPLEVCCGSYYGYR 321
Q FN +LK V Q LP +K YV+ Y ++ISN S GF CCG
Sbjct: 830 NQIFNNKLKGLVDQFNNQLPDSKVIYVNSYGIFQDIISNPSAYGFSVTNAGCCG------ 991
Query: 322 IDCGKKAVVNGTV----YGNPCKNPSQHISWDGVHYTQAANKWVAKHIRDGSLSDPPVPI 377
G+ NG PC+N +++ WD H T+A N VA+ D PI
Sbjct: 992 --VGRN---NGQFTCLPLQTPCENRREYLFWDAFHPTEAGNVVVAQRAYSAQSPDDAYPI 1156
>TC76727 similar to GP|10638955|emb|CAB81548. putative proline-rich protein
APG isolog {Cicer arietinum}, partial (92%)
Length = 1919
Score = 103 bits (257), Expect = 1e-22
Identities = 94/343 (27%), Positives = 150/343 (43%), Gaps = 17/343 (4%)
Frame = -2
Query: 40 PAVYNFGDSNSDTG---VVYAAFAGLQSPGGISFFGNL-SGRASDGRLIIDFITEELEIP 95
PA+ FGDS D G + F P G F +GR +G+L DF E L
Sbjct: 1090 PAIMTFGDSAVDVGNNDYLPTLFKANYPPYGRDFTNKQPTGRFCNGKLATDFTAETLGFT 911
Query: 96 -YLSAYLN--SIGSNYRHGANFA--AGGASIRPVYGFSPFYLGMQVAQFIQLQSHIENLL 150
+ AYL+ + G N GANFA A G + L Q+ F + Q + +
Sbjct: 910 SFAPAYLSPQASGKNLLLGANFASAASGYDEKAATLNHAIPLSQQLEYFKEYQGKLAQVA 731
Query: 151 NQFSSNRTEPPFKSYLPRPEDFSKALYTIDIGQNDLGFGLMHTSEEEVLRSIPE------ 204
S + K +LY + G +D F + + + ++I
Sbjct: 730 G---SKKAASIIKD----------SLYVLSAGSSD--FVQNYYTNPWINQAITVDQYSSY 596
Query: 205 MMRNFTYDVQVLYDVGARVFWIHNTGPIGCLPTSSIFYEPKKGNLDANGCVIPHNKIAQE 264
++ +FT ++ +Y +GAR + + P+GCLP + + NGCV N AQ
Sbjct: 595 LLDSFTNFIKGVYGLGARKIGVTSLPPLGCLPAARTLF-----GYHENGCVARINTDAQG 431
Query: 265 FNRQLKDQVFQLRRNLPKAKFTYVDVYTAKYELISNASKQGFVNPLEVCCGS--YYGYRI 322
FN+++ L++ LP K D+Y Y+L+ N S GF + CCG+ +
Sbjct: 430 FNKKVSSAASNLQKQLPGLKIVIFDIYKPLYDLVQNPSNFGFAEAGKGCCGTGLVETTSL 251
Query: 323 DCGKKAVVNGTVYGNPCKNPSQHISWDGVHYTQAANKWVAKHI 365
C K++ GT C N +Q++ WD VH ++AAN+ +A ++
Sbjct: 250 LCNPKSL--GT-----CSNATQYVFWDSVHPSEAANQVLADNL 143
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.321 0.138 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,841,424
Number of Sequences: 36976
Number of extensions: 192146
Number of successful extensions: 1165
Number of sequences better than 10.0: 93
Number of HSP's better than 10.0 without gapping: 1079
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1088
length of query: 382
length of database: 9,014,727
effective HSP length: 98
effective length of query: 284
effective length of database: 5,391,079
effective search space: 1531066436
effective search space used: 1531066436
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0400a.7