BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0400a.6
(619 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC77406 homologue to SP|O82663|DHSA_ARATH Succinate dehydrogenas... 169 4e-42
BQ148255 similar to GP|15010650|gb| AT5g14760/T9L3_60 {Arabidops... 155 6e-38
TC77407 homologue to SP|O82663|DHSA_ARATH Succinate dehydrogenas... 69 5e-12
BQ138479 weakly similar to PIR|T37806|T37 probable flavoprotein ... 50 3e-06
TC86404 homologue to PIR|T07126|T07126 magnesium chelatase (EC 4... 32 0.72
AL375259 weakly similar to GP|8843752|dbj| gene_id:MXK3.11~unkno... 31 1.2
TC90236 similar to GP|4586308|dbj|BAA76348.1 protoporphyrinogen ... 31 1.2
TC87665 weakly similar to GP|17529192|gb|AAL38822.1 unknown prot... 31 1.6
TC80583 squalene monooxygenase 1 [Medicago truncatula] 30 2.7
TC88192 weakly similar to PIR|T05682|T05682 monooxygenase (EC 1.... 29 4.7
AL367111 homologue to GP|19550437|gb| carotenoid isomerase {Lyco... 29 6.1
AW584299 similar to GP|20160694|db hypothetical protein~similar ... 29 6.1
BG584080 similar to GP|4204303|gb| lcl|prt_seq No definition lin... 29 6.1
BQ135590 similar to GP|23499166|em hypothetical protein {Plasmod... 28 8.0
>TC77406 homologue to SP|O82663|DHSA_ARATH Succinate dehydrogenase
[ubiquinone] flavoprotein subunit mitochondrial (EC
1.3.5.1) (FP), partial (65%)
Length = 1546
Score = 169 bits (427), Expect = 4e-42
Identities = 134/421 (31%), Positives = 196/421 (45%), Gaps = 31/421 (7%)
Frame = +2
Query: 224 GADILNTETLEVVRFLSKVTLLASGGAGHIYPKTTNPLVATGDGIAMAHRAQAVISNMEF 283
G LN E + RF + T+LA+GG G Y T+ TGDG AMA RA + ++EF
Sbjct: 5 GVIALNMEDGTLHRFQAASTILATGGYGRAYFSATSAHTCTGDGNAMAARAGIGLEDLEF 184
Query: 284 VQFHPTALADEGLPIKPTKLRDNAFLITEAVRGDGGILYNLGMERFMPLYDERA-ELAPR 342
VQFHPT + G LITE RG+GGIL N ERFM Y A +LA R
Sbjct: 185 VQFHPTGIYGAGC------------LITEGSRGEGGILRNSEGERFMERYAPTAKDLASR 328
Query: 343 DVVARSIDDQLKK-----RDDKYVLLDISHKPKEEILSHFPNIASFCLQY-GLDITRHPI 396
DVV+RS+ ++++ + ++ L ++H P + + P I+ + G+D+T+ PI
Sbjct: 329 DVVSRSMTMEIREGRGVGPEKDHIYLHLNHLPPDVLKERLPGISETAAIFAGVDVTKEPI 508
Query: 397 PVVPAAHYMCGGVRAGLEGE----------TNVQGLYVAGEVACTGLHGANRLASNSLLE 446
PV+P HY GG+ GE V GL AGE AC +HGANRL +NSLL+
Sbjct: 509 PVLPTVHYNMGGIPTNHHGEVVTIKGDDPDALVPGLMAAGEAACASVHGANRLGANSLLD 688
Query: 447 ALVFAR----RAVQPSVDQMKSSSLDLTASNLWPRPIVPLSLESDVMNKILSL-TKELRK 501
+VF R R + K L+ A ++ + N SL T ++R
Sbjct: 689 IVVFGRACANRVAEIHKPGEKQKPLEKDAGQ------KTIAWLDKLRNSNGSLPTSKIRL 850
Query: 502 ELQSIMWYYVGIVRSTMRLETAELKIGNLEAKWEEYLFQHG---------WKPTMVAPEI 552
+Q +M + R+ ET E ++ WE + H W ++ E
Sbjct: 851 NMQKVMQNNAAVFRTQ---ETLEEGCQLIDKTWESF---HDVKVEDRSLIWNSDLI--ET 1006
Query: 553 CEMRNLFCCAKLVVSSALSRHESRGLHYTIDFPYLEESERLPTIIFPSSRVKSTRSFRQL 612
E+ NL A + + SA +R ESRG H DF ++ + + + K +R +
Sbjct: 1007IELENLLINACVTMHSAEARKESRGAHAREDFTTRDDEKWMKHTLGFWENEKVRLDYRPV 1186
Query: 613 H 613
H
Sbjct: 1187H 1189
>BQ148255 similar to GP|15010650|gb| AT5g14760/T9L3_60 {Arabidopsis
thaliana}, partial (12%)
Length = 575
Score = 155 bits (391), Expect = 6e-38
Identities = 76/94 (80%), Positives = 79/94 (83%)
Frame = -2
Query: 526 KIGNLEAKWEEYLFQHGWKPTMVAPEICEMRNLFCCAKLVVSSALSRHESRGLHYTIDFP 585
KIGN LF+HGWKPTMV PEICEMRNLFCCAKLVVSSALSRHESRGLHYTIDFP
Sbjct: 574 KIGNXRRNGRSILFRHGWKPTMVVPEICEMRNLFCCAKLVVSSALSRHESRGLHYTIDFP 395
Query: 586 YLEESERLPTIIFPSSRVKSTRSFRQLHKQPICQ 619
LEESERLPTIIFPSS + ST SFRQL KQP+ Q
Sbjct: 394 NLEESERLPTIIFPSSPLISTWSFRQLQKQPMYQ 293
>TC77407 homologue to SP|O82663|DHSA_ARATH Succinate dehydrogenase
[ubiquinone] flavoprotein subunit mitochondrial (EC
1.3.5.1) (FP), partial (30%)
Length = 833
Score = 68.9 bits (167), Expect = 5e-12
Identities = 40/108 (37%), Positives = 63/108 (58%), Gaps = 3/108 (2%)
Frame = +1
Query: 59 FDFVVIGSGIAGLRYALEVAKYG-SVAVITKAESHECNTNYAQGGVSAVL--CPSDSVEN 115
+D VV+G+G AGLR A+ ++++G + A ITK +T AQGG++A L D
Sbjct: 280 YDAVVVGAGGAGLRAAIGLSEHGFNTACITKLFPTRSHTVAAQGGINAALGNMTEDDWRW 459
Query: 116 HMKDTIVAGAYLCDEESVRVVCTEGPERVRELIAMGASFDHGEDGNLH 163
HM DT+ +L D+++++ +C E P+ V EL G F EDG ++
Sbjct: 460 HMYDTVKGSDWLGDQDAIQYMCREAPKAVIELENYGLPFSRTEDGRIY 603
>BQ138479 weakly similar to PIR|T37806|T37 probable flavoprotein subunit -
fission yeast (Schizosaccharomyces pombe), partial (22%)
Length = 564
Score = 49.7 bits (117), Expect = 3e-06
Identities = 33/73 (45%), Positives = 39/73 (53%)
Frame = +3
Query: 257 TTNPLVATGDGIAMAHRAQAVISNMEFVQFHPTALADEGLPIKPTKLRDNAFLITEAVRG 316
TTN ATGDG M + A +M+ VQ HPT L D P T FL EA+RG
Sbjct: 285 TTNGAHATGDGHKMLMKIGAQGIDMDKVQVHPTGLIDPKDPDAKT-----XFLAVEALRG 449
Query: 317 DGGILYNLGMERF 329
+GGIL N +RF
Sbjct: 450 EGGILLNGKGQRF 488
>TC86404 homologue to PIR|T07126|T07126 magnesium chelatase (EC 4.99.1.-)
chain chlH - soybean, partial (61%)
Length = 2997
Score = 32.0 bits (71), Expect = 0.72
Identities = 16/53 (30%), Positives = 24/53 (45%), Gaps = 1/53 (1%)
Frame = -1
Query: 356 RDDKYVL-LDISHKPKEEILSHFPNIASFCLQYGLDITRHPIPVVPAAHYMCG 407
RD YVL L + H + + +FP ++ FCL L T + + CG
Sbjct: 1551 RDSPYVLILSVPHSTRATVSGYFPPLSIFCLSSNLSTTILALSIAAVVGIDCG 1393
>AL375259 weakly similar to GP|8843752|dbj| gene_id:MXK3.11~unknown protein
{Arabidopsis thaliana}, partial (22%)
Length = 466
Score = 31.2 bits (69), Expect = 1.2
Identities = 18/68 (26%), Positives = 32/68 (46%)
Frame = +3
Query: 202 VFEHHFAIDLLTSQDGSDIICLGADILNTETLEVVRFLSKVTLLASGGAGHIYPKTTNPL 261
+ HH A D T+ S + + LNT L++ R + +++ G I +T+ +
Sbjct: 48 LLHHHHASDHTTADSSSTLHSTSSVTLNTNPLQITRAVENISI-----KGEIPTRTSREI 212
Query: 262 VATGDGIA 269
V +GIA
Sbjct: 213 VREVEGIA 236
>TC90236 similar to GP|4586308|dbj|BAA76348.1 protoporphyrinogen IX
oxidase {Glycine max}, partial (39%)
Length = 686
Score = 31.2 bits (69), Expect = 1.2
Identities = 14/45 (31%), Positives = 26/45 (57%)
Frame = +1
Query: 46 ISSSCKKDDPTKYFDFVVIGSGIAGLRYALEVAKYGSVAVITKAE 90
++SS K D+P V+G+G++GL A ++ +G + +AE
Sbjct: 16 MASSAKDDNPRSVKRVAVVGAGVSGLAAAYKLKSHGLDVTVFEAE 150
>TC87665 weakly similar to GP|17529192|gb|AAL38822.1 unknown protein
{Arabidopsis thaliana}, partial (80%)
Length = 1446
Score = 30.8 bits (68), Expect = 1.6
Identities = 17/58 (29%), Positives = 26/58 (44%)
Frame = -1
Query: 163 HLMREGGHSHRRIVHAADMTGKEIERALLKAAVNNPNIFVFEHHFAIDLLTSQDGSDI 220
H+ E R + T K + ++ L+A + +I HH D L SQDGS +
Sbjct: 756 HIWAESAP*MRSTPCKSPSTDKHLSKSALEAQASTNSILCSAHHSKHDFLMSQDGSSL 583
>TC80583 squalene monooxygenase 1 [Medicago truncatula]
Length = 1830
Score = 30.0 bits (66), Expect = 2.7
Identities = 12/51 (23%), Positives = 26/51 (50%)
Frame = +3
Query: 43 VKVISSSCKKDDPTKYFDFVVIGSGIAGLRYALEVAKYGSVAVITKAESHE 93
+ + C+ +P D +++G+G+AG A + K G +I + + +E
Sbjct: 231 ITTATGECRSFNPNGDVDIIIVGAGVAGSALAYTLGKDGRRVLIIERDLNE 383
>TC88192 weakly similar to PIR|T05682|T05682 monooxygenase (EC 1.-.-.-) 2 -
Arabidopsis thaliana, partial (61%)
Length = 1557
Score = 29.3 bits (64), Expect = 4.7
Identities = 17/51 (33%), Positives = 29/51 (56%), Gaps = 3/51 (5%)
Frame = +2
Query: 54 DPTKYF---DFVVIGSGIAGLRYALEVAKYGSVAVITKAESHECNTNYAQG 101
+ TK F D V++G+GIAGL AL + + G +++ ++ T +A G
Sbjct: 95 ETTKIFEEEDVVIVGAGIAGLATALGLHRLGVRSLVLESSDSLRVTGFALG 247
>AL367111 homologue to GP|19550437|gb| carotenoid isomerase {Lycopersicon
esculentum}, partial (4%)
Length = 293
Score = 28.9 bits (63), Expect = 6.1
Identities = 17/42 (40%), Positives = 24/42 (56%)
Frame = +2
Query: 41 KLVKVISSSCKKDDPTKYFDFVVIGSGIAGLRYALEVAKYGS 82
KL + + S K+ D + FD VIGSGI GL ++A G+
Sbjct: 161 KLEEEVVESGKRGDFGEGFDAXVIGSGIGGLVAGTQLAVKGA 286
>AW584299 similar to GP|20160694|db hypothetical protein~similar to
Arabidopsis thaliana chromosome 3 At3g56840, partial
(22%)
Length = 549
Score = 28.9 bits (63), Expect = 6.1
Identities = 28/109 (25%), Positives = 41/109 (36%), Gaps = 1/109 (0%)
Frame = +2
Query: 55 PTKYFDFVVIGSGIAGLRYALEVAKYG-SVAVITKAESHECNTNYAQGGVSAVLCPSDSV 113
P + D VVIG+G+ G+ A +A G V VI A S T+
Sbjct: 134 PRERVDCVVIGAGVVGIAVARALALKGREVIVIESAPSFGTGTS---------------- 265
Query: 114 ENHMKDTIVAGAYLCDEESVRVVCTEGPERVRELIAMGASFDHGEDGNL 162
+ + + AG Y + C +G E + E A H + G L
Sbjct: 266 -SRNSEVVHAGIYYPHHSLKAIFCVKGREMLYEYCAK-HDIPHEQTGKL 406
>BG584080 similar to GP|4204303|gb| lcl|prt_seq No definition line found
{Arabidopsis thaliana}, partial (28%)
Length = 771
Score = 28.9 bits (63), Expect = 6.1
Identities = 20/69 (28%), Positives = 34/69 (48%)
Frame = +1
Query: 476 PRPIVPLSLESDVMNKILSLTKELRKELQSIMWYYVGIVRSTMRLETAELKIGNLEAKWE 535
P+ + L S KI+S+T+ + +I V ++ S +R + L++ L KW
Sbjct: 223 PQETMEKKLVSSKRYKIISMTQVQVHLVHAIAM--VPLINSKIRRKETFLELQILLLKWL 396
Query: 536 EYLFQHGWK 544
YL QH W+
Sbjct: 397 HYLQQH*WQ 423
>BQ135590 similar to GP|23499166|em hypothetical protein {Plasmodium
falciparum 3D7}, partial (0%)
Length = 1238
Score = 28.5 bits (62), Expect = 8.0
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = +2
Query: 398 VVPAAHYMCGGVRAGLEGET 417
+VPA H+ CG +R+G E E+
Sbjct: 218 IVPAVHFSCGNMRSGWEVES 277
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.320 0.136 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,010,111
Number of Sequences: 36976
Number of extensions: 261591
Number of successful extensions: 1127
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 1117
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1120
length of query: 619
length of database: 9,014,727
effective HSP length: 102
effective length of query: 517
effective length of database: 5,243,175
effective search space: 2710721475
effective search space used: 2710721475
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0400a.6