BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0391.5
(359 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC80729 auxin efflux carrier protein [Medicago truncatula] 221 3e-58
NP565645 NP565645|AY115840.1|AAM55301.1 auxin efflux carrier pro... 220 6e-58
TC91788 auxin efflux carrier protein [Medicago truncatula] 202 2e-52
TC81304 auxin efflux carrier protein [Medicago truncatula] 193 1e-49
TC83905 auxin efflux carrier protein [Medicago truncatula] 188 2e-48
TC90862 similar to GP|21740496|emb|CAD40820. OSJNBa0006B20.11 {O... 33 0.17
TC92370 similar to GP|21780148|gb|AAM77646.1 hypothetical protei... 32 0.49
AW692007 weakly similar to PIR|C84527|C845 probable receptor-lik... 30 1.4
BM813642 weakly similar to PIR|F84633|F846 probable carbonyl red... 29 3.2
TC87464 homologue to GP|7635498|emb|CAB88668.1 histone H2B {Cice... 28 4.2
TC82753 similar to GP|9758920|dbj|BAB09457.1 gb|AAD25781.1~gene_... 28 5.5
TC86831 similar to GP|14334654|gb|AAK59505.1 putative formamidas... 28 5.5
AW694815 similar to GP|22091415|gb putative RNA helicase {Arabid... 28 7.1
TC79146 similar to GP|9757884|dbj|BAB08471.1 RuvB DNA helicase-l... 27 9.3
TC79621 MtN3 27 9.3
TC82418 similar to PIR|C86204|C86204 hypothetical protein [impor... 27 9.3
>TC80729 auxin efflux carrier protein [Medicago truncatula]
Length = 2366
Score = 221 bits (564), Expect = 3e-58
Identities = 112/187 (59%), Positives = 138/187 (72%)
Frame = +1
Query: 173 GESETSQELQSKEEEEAEHRPGRKLRILLILTKVGKKLIKNPNTYATFIGLIWSSIHFRW 232
G S ++ EL +H P + LIL V +KLI+NPNTY++ IGLIWS + FRW
Sbjct: 1423 GGSSSTAELHPTAT--VKHMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLIWSLVAFRW 1596
Query: 233 GVHMPDVVNQSITILSSGGLGMATFSLGLFMASNSTIIACGPRMTMVAIGLKFLVGPALM 292
VHMP ++ +SI+ILS GLGMA FSLGLFMA +IACG + A+ ++FL GPA+M
Sbjct: 1597 HVHMPKIIEKSISILSDAGLGMAMFSLGLFMALQPKMIACGNSVASFAMAVRFLTGPAVM 1776
Query: 293 AVASIVIGLRDTMLKVAIVQAALPQGIVPFVFAREYNVHPAILSTGVLLGMLIALPVALT 352
A ASI +GLR +L+VAIVQAALPQGIVPFVFA+EYNVHPAILSTGV+ GMLIALP+ L
Sbjct: 1777 AAASIAVGLRGNLLRVAIVQAALPQGIVPFVFAKEYNVHPAILSTGVIFGMLIALPITLV 1956
Query: 353 YYVLLAL 359
YY+LL L
Sbjct: 1957 YYILLGL 1977
Score = 187 bits (476), Expect = 4e-48
Identities = 90/157 (57%), Positives = 120/157 (76%)
Frame = +1
Query: 1 MISLGDVYHVVTATVPLYVTMILAFISVKWWKLFTPDQCSGINKFVANFSIPLLSFQVIS 60
MI+ D+Y V+TA VPLYV MILA+ SV+WWK+F+PDQCSGIN+FVA F++PLLSF IS
Sbjct: 1 MITWHDLYTVLTAVVPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 180
Query: 61 SNNVYKMSLKLIYADCIQKLLAILALTLIIKISARGGLKWIITGLSLSTLPNTLILGIPL 120
+NN Y M+ + I AD +QK++ +LALT+ +A G L+W+IT SLSTLPNTL++GIPL
Sbjct: 181 TNNPYAMNYRFIAADTLQKIIMLLALTIWTNFTANGSLEWMITIFSLSTLPNTLVMGIPL 360
Query: 121 MKAMYKDEADALLPQIIFLQSMIWYNLLLFLHELDAA 157
+ AMY + + L+ Q++ LQ +IWY LLLFL E A
Sbjct: 361 LIAMYGEYSGTLMVQVVVLQCIIWYTLLLFLFEYRGA 471
>NP565645 NP565645|AY115840.1|AAM55301.1 auxin efflux carrier protein [Medicago
truncatula]
Length = 1575
Score = 220 bits (561), Expect = 6e-58
Identities = 112/184 (60%), Positives = 136/184 (73%)
Frame = +1
Query: 176 ETSQELQSKEEEEAEHRPGRKLRILLILTKVGKKLIKNPNTYATFIGLIWSSIHFRWGVH 235
E E + EE A+ P + LIL V +KLI+NPNTY++ IGL WS I FRW +
Sbjct: 1021 EGEDEHKRGEEGGAKTMPPASIMTRLILIMVWRKLIRNPNTYSSIIGLTWSLISFRWNIE 1200
Query: 236 MPDVVNQSITILSSGGLGMATFSLGLFMASNSTIIACGPRMTMVAIGLKFLVGPALMAVA 295
MP ++ +SI+ILS GLGMA FSLGLFMA IIACG R+ ++ ++FL GPA+MA A
Sbjct: 1201 MPVIIAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNRIAAFSMAIRFLTGPAVMAAA 1380
Query: 296 SIVIGLRDTMLKVAIVQAALPQGIVPFVFAREYNVHPAILSTGVLLGMLIALPVALTYYV 355
SIV+GLR T+L VAIVQAALPQGIVPFVFA+EYNVHP ILSTGV+ GMLIALP+ L YY+
Sbjct: 1381 SIVVGLRGTLLNVAIVQAALPQGIVPFVFAKEYNVHPDILSTGVIFGMLIALPITLVYYI 1560
Query: 356 LLAL 359
LL L
Sbjct: 1561 LLGL 1572
Score = 192 bits (489), Expect = 1e-49
Identities = 92/158 (58%), Positives = 123/158 (77%), Gaps = 1/158 (0%)
Frame = +1
Query: 1 MISLGDVYHVVTATVPLYVTMILAFISVKWWKLFTPDQCSGINKFVANFSIPLLSFQVIS 60
MI+L D YHV+T+ VPLYV MILA+ SVKWWK+F+PDQCSGIN+FVA F++PLLSF I+
Sbjct: 1 MITLTDFYHVMTSMVPLYVAMILAYGSVKWWKIFSPDQCSGINRFVALFAVPLLSFHFIA 180
Query: 61 SNNVYKMSLKLIYADCIQKLLAILALTLIIKISARGGLKWIITGLSLSTLPNTLILGIPL 120
SNN YKM+ + + AD +QK++ +LALT+ +S RG L+W IT S+STLPNTL++GIPL
Sbjct: 181 SNNPYKMNTRFLVADTLQKIIVLLALTIWANVSKRGCLEWTITLFSISTLPNTLVMGIPL 360
Query: 121 MKAMYKDE-ADALLPQIIFLQSMIWYNLLLFLHELDAA 157
+K MY E + +L+ QI+ LQ +IWY L+LF+ E A
Sbjct: 361 LKCMYGGEFSGSLMVQIVVLQCIIWYTLILFMFEFRGA 474
>TC91788 auxin efflux carrier protein [Medicago truncatula]
Length = 2076
Score = 202 bits (514), Expect = 2e-52
Identities = 99/167 (59%), Positives = 126/167 (75%)
Frame = +1
Query: 193 PGRKLRILLILTKVGKKLIKNPNTYATFIGLIWSSIHFRWGVHMPDVVNQSITILSSGGL 252
P + LIL V +KLI+NPNTY++ IGL+WS + FR+ + MP +V SI+ILS GL
Sbjct: 1375 PPASVMTRLILIMVWRKLIRNPNTYSSLIGLVWSLVSFRFHIEMPSIVKGSISILSDAGL 1554
Query: 253 GMATFSLGLFMASNSTIIACGPRMTMVAIGLKFLVGPALMAVASIVIGLRDTMLKVAIVQ 312
GMA FSLGLFMA +IACG R+ ++ ++FL GPA++A SI IG+R +L VAIVQ
Sbjct: 1555 GMAMFSLGLFMALQPKLIACGKRVATFSMAVRFLTGPAVIAATSIAIGIRGVLLHVAIVQ 1734
Query: 313 AALPQGIVPFVFAREYNVHPAILSTGVLLGMLIALPVALTYYVLLAL 359
AALPQGIVPFVFA+EYN+HP ILSTGV+ GMLIALP+ + YYVLL +
Sbjct: 1735 AALPQGIVPFVFAKEYNLHPNILSTGVIFGMLIALPITILYYVLLGV 1875
Score = 172 bits (436), Expect = 2e-43
Identities = 89/158 (56%), Positives = 115/158 (72%), Gaps = 1/158 (0%)
Frame = +1
Query: 1 MISLGDVYHVVTATVPLYVTMILAFISVKWWKLFTPDQCSGINKFVANFSIPLLSFQVIS 60
MI+ D+Y V A VPLYV MILA+ SV+WWK+FTPDQCSGIN+FVA F++PLLSF IS
Sbjct: 1 MITGKDIYDVFAAIVPLYVAMILAYGSVRWWKIFTPDQCSGINRFVAVFAVPLLSFHFIS 180
Query: 61 SNNVYKMSLKLIYADCIQKLLAILALTLIIKISA-RGGLKWIITGLSLSTLPNTLILGIP 119
SN+ Y M+ + AD +QK++ + AL + S + L W IT SLSTLPNTL++GIP
Sbjct: 181 SNDPYAMNYHFLAADSLQKVVILGALFIWNTFSKNQDSLDWTITLFSLSTLPNTLVMGIP 360
Query: 120 LMKAMYKDEADALLPQIIFLQSMIWYNLLLFLHELDAA 157
L++AMY D + L+ QI+ LQS+IWY L+LFL E AA
Sbjct: 361 LLRAMYGDFSGNLMVQIVVLQSVIWYTLMLFLFEYRAA 474
>TC81304 auxin efflux carrier protein [Medicago truncatula]
Length = 831
Score = 193 bits (490), Expect = 1e-49
Identities = 92/148 (62%), Positives = 116/148 (78%)
Frame = +3
Query: 212 KNPNTYATFIGLIWSSIHFRWGVHMPDVVNQSITILSSGGLGMATFSLGLFMASNSTIIA 271
+NPNTY++ IGL WS + FR+ + MP ++ +SI+ILS GLGMA FSLGLFMA IIA
Sbjct: 3 RNPNTYSSLIGLTWSLVSFRYHIEMPAIIAKSISILSDAGLGMAMFSLGLFMALQPKIIA 182
Query: 272 CGPRMTMVAIGLKFLVGPALMAVASIVIGLRDTMLKVAIVQAALPQGIVPFVFAREYNVH 331
CG + ++G++FLVGPA+MA AS +GL+ + VAIVQAALPQGIVPFVFA+EYNVH
Sbjct: 183 CGNSIAAFSMGVRFLVGPAVMAAASFAVGLKGVLFHVAIVQAALPQGIVPFVFAKEYNVH 362
Query: 332 PAILSTGVLLGMLIALPVALTYYVLLAL 359
P ILSTGV+ GMLIALP+ L YY+L+ L
Sbjct: 363 PDILSTGVIFGMLIALPITLVYYILMGL 446
>TC83905 auxin efflux carrier protein [Medicago truncatula]
Length = 1869
Score = 188 bits (478), Expect = 2e-48
Identities = 90/157 (57%), Positives = 122/157 (77%)
Frame = +1
Query: 1 MISLGDVYHVVTATVPLYVTMILAFISVKWWKLFTPDQCSGINKFVANFSIPLLSFQVIS 60
MI+L D+Y V+TA VPLYV MILA+ SV+WWK+F+PDQCSGIN+FVA F++PLLSF IS
Sbjct: 1 MITLKDLYTVLTAVVPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAVFAVPLLSFHFIS 180
Query: 61 SNNVYKMSLKLIYADCIQKLLAILALTLIIKISARGGLKWIITGLSLSTLPNTLILGIPL 120
+NN Y+M+ + I AD +QK++ ++AL+L + G L+W+IT SLSTLPNTL++GIPL
Sbjct: 181 TNNPYQMNFRFIAADTLQKIIMLVALSLWTLFTKNGNLEWMITIFSLSTLPNTLVMGIPL 360
Query: 121 MKAMYKDEADALLPQIIFLQSMIWYNLLLFLHELDAA 157
+ AMY D + +L+ Q++ LQ +IWY LLLFL E A
Sbjct: 361 LIAMYGDYSGSLMVQVVVLQCIIWYTLLLFLFEYRGA 471
Score = 173 bits (439), Expect = 8e-44
Identities = 101/191 (52%), Positives = 122/191 (62%), Gaps = 6/191 (3%)
Frame = +1
Query: 175 SETSQELQSKEEEEAEHRPGR-----KLRILLILTKVGKKLIKNPNTYATFIGLIWSSIH 229
S ++ EL K A+ G+ + LIL V +KLI+NPNTY++ IGLI
Sbjct: 1294 SNSTPELHPKATGVADSSVGKLMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLI*VPCC 1473
Query: 230 FRWGVHMP-DVVNQSITILSSGGLGMATFSLGLFMASNSTIIACGPRMTMVAIGLKFLVG 288
G + S GLGMA FSLGLFMA IIACG + A+ ++FL G
Sbjct: 1474 L*VGCSYA*NNRRNQFPYCSDAGLGMAMFSLGLFMALQPKIIACGNSVASFAMAIRFLTG 1653
Query: 289 PALMAVASIVIGLRDTMLKVAIVQAALPQGIVPFVFAREYNVHPAILSTGVLLGMLIALP 348
PA+MA ASI +GLR T+L VAIVQAALPQGIVPFVFA+EYNVHPAILST V+ GMLIALP
Sbjct: 1654 PAVMAAASIAVGLRGTLLHVAIVQAALPQGIVPFVFAKEYNVHPAILSTAVIFGMLIALP 1833
Query: 349 VALTYYVLLAL 359
+ L YY+LL L
Sbjct: 1834 ITLLYYILLGL 1866
>TC90862 similar to GP|21740496|emb|CAD40820. OSJNBa0006B20.11 {Oryza
sativa}, partial (14%)
Length = 1090
Score = 33.1 bits (74), Expect = 0.17
Identities = 17/49 (34%), Positives = 31/49 (62%)
Frame = +1
Query: 23 LAFISVKWWKLFTPDQCSGINKFVANFSIPLLSFQVISSNNVYKMSLKL 71
L F+S ++ LF+ + I +F NFSIP++ + ++ S+ +YK SL +
Sbjct: 25 LHFLSFSFYTLFSVRK---ILQF*INFSIPIIQYGILVSSQLYKFSLPI 162
>TC92370 similar to GP|21780148|gb|AAM77646.1 hypothetical protein
{Arabidopsis thaliana}, partial (15%)
Length = 577
Score = 31.6 bits (70), Expect = 0.49
Identities = 19/59 (32%), Positives = 36/59 (60%), Gaps = 8/59 (13%)
Frame = +1
Query: 23 LAFISVKWWKLFTPDQCS-----GINKFVANFSIPLLSFQVISSN---NVYKMSLKLIY 73
+ ++++++++LF Q S I +F+AN I ++ +I +N NVYK++L LIY
Sbjct: 343 IVWVTLEYFQLF*SIQNSILKSNSIPRFIANMCIVIIQLSLIIANP*ANVYKVTLYLIY 519
>AW692007 weakly similar to PIR|C84527|C845 probable receptor-like protein
kinase [imported] - Arabidopsis thaliana, partial (10%)
Length = 510
Score = 30.0 bits (66), Expect = 1.4
Identities = 18/44 (40%), Positives = 24/44 (53%), Gaps = 3/44 (6%)
Frame = +1
Query: 50 SIPLLSFQVISSNNVYK---MSLKLIYADCIQKLLAILALTLII 90
S PLLS V+SSNNV K + + LL +L+LTL +
Sbjct: 46 SFPLLSLSVLSSNNVQKPLPSQISKTMKTTLSLLLTLLSLTLTL 177
>BM813642 weakly similar to PIR|F84633|F846 probable carbonyl reductase
[imported] - Arabidopsis thaliana, partial (32%)
Length = 560
Score = 28.9 bits (63), Expect = 3.2
Identities = 28/118 (23%), Positives = 57/118 (47%), Gaps = 11/118 (9%)
Frame = +1
Query: 7 VYHVVTATVPLYVTMILAFISVKWWKLFTPDQCSGINKFVANFSI-PLLSFQVISSNNVY 65
V+H + T P + ++ F+ ++ +L +GIN F A+ + P+++++ +S Y
Sbjct: 205 VFHQLDVTDPFSIASLVEFVKTQFGRLDILVNNAGINGFNADDMVEPIINWRELS--QTY 378
Query: 66 KMSLKLIYA------DCIQKLLAILALT----LIIKISARGGLKWIITGLSLSTLPNT 113
+M+ I + + L +L L+ ++ SA G LK+I + S L +T
Sbjct: 379 EMAENCIITNYYGGKETTEAFLPLLQLSDSPVIVNGSSAAGLLKYISNEWARSVLNDT 552
>TC87464 homologue to GP|7635498|emb|CAB88668.1 histone H2B {Cicer
arietinum}, complete
Length = 677
Score = 28.5 bits (62), Expect = 4.2
Identities = 21/89 (23%), Positives = 41/89 (45%)
Frame = +3
Query: 146 NLLLFLHELDAAIPARTMPATPPSQGTGESETSQELQSKEEEEAEHRPGRKLRILLILTK 205
+LL+F+H TMP T + E + +++ ++++ +AE +K+ K
Sbjct: 6 HLLIFIHLFPNLSNQSTMPPTKAEKKPAEKKPAEKAPAEKKPKAE----KKISKEGSSDK 173
Query: 206 VGKKLIKNPNTYATFIGLIWSSIHFRWGV 234
K+ K+ TY +I + +H GV
Sbjct: 174 KKKRTKKSVETYKIYIFKVLKQVHPDIGV 260
>TC82753 similar to GP|9758920|dbj|BAB09457.1
gb|AAD25781.1~gene_id:MXI22.10~strong similarity to
unknown protein {Arabidopsis thaliana}, partial (14%)
Length = 816
Score = 28.1 bits (61), Expect = 5.5
Identities = 12/42 (28%), Positives = 24/42 (56%)
Frame = +2
Query: 270 IACGPRMTMVAIGLKFLVGPALMAVASIVIGLRDTMLKVAIV 311
+ C TMV +GL ++G +M V ++IG + +L + ++
Sbjct: 494 VRCLSVRTMVRVGLMIILGVLVMRVILVLIGFMNVVLVLVMI 619
>TC86831 similar to GP|14334654|gb|AAK59505.1 putative formamidase
{Arabidopsis thaliana}, partial (96%)
Length = 1558
Score = 28.1 bits (61), Expect = 5.5
Identities = 13/40 (32%), Positives = 23/40 (57%)
Frame = -2
Query: 136 IIFLQSMIWYNLLLFLHELDAAIPARTMPATPPSQGTGES 175
++FL + W N+ + + + A+ A T+P PSQG +S
Sbjct: 1260 LLFLGRISWSNIAVGMANVAQALGASTIPDILPSQGQQDS 1141
>AW694815 similar to GP|22091415|gb putative RNA helicase {Arabidopsis
thaliana}, partial (16%)
Length = 404
Score = 27.7 bits (60), Expect = 7.1
Identities = 8/16 (50%), Positives = 13/16 (81%)
Frame = -1
Query: 31 WKLFTPDQCSGINKFV 46
WK+ TP QC+G N+++
Sbjct: 233 WKIHTPSQCAGTNQYL 186
>TC79146 similar to GP|9757884|dbj|BAB08471.1 RuvB DNA helicase-like protein
{Arabidopsis thaliana}, partial (47%)
Length = 792
Score = 27.3 bits (59), Expect = 9.3
Identities = 19/63 (30%), Positives = 36/63 (56%)
Frame = -3
Query: 289 PALMAVASIVIGLRDTMLKVAIVQAALPQGIVPFVFAREYNVHPAILSTGVLLGMLIALP 348
P++ +V+S++ L L+ A V+A++ F +RE + PAI++ GV A+P
Sbjct: 526 PSITSVSSLI--LTPIALRKACVKASV------FDISREKSSLPAIMANGVSRPRDFAIP 371
Query: 349 VAL 351
+A+
Sbjct: 370 IAI 362
>TC79621 MtN3
Length = 1117
Score = 27.3 bits (59), Expect = 9.3
Identities = 23/106 (21%), Positives = 48/106 (44%)
Frame = +1
Query: 54 LSFQVISSNNVYKMSLKLIYADCIQKLLAILALTLIIKISARGGLKWIITGLSLSTLPNT 113
L+F+++S+ NV +L LI + A+ +++ G W+ LS+S
Sbjct: 412 LTFKLLSAMNVGSFALILIVTN--------YAVHGPLRVQVLG---WVCVSLSVSVFAAP 558
Query: 114 LILGIPLMKAMYKDEADALLPQIIFLQSMIWYNLLLFLHELDAAIP 159
L + +++ + L + L + +W+ LFL ++ A+P
Sbjct: 559 LSIVAQVVRTKSVEFMPFNLSFTLTLSATMWFGYGLFLKDICIALP 696
>TC82418 similar to PIR|C86204|C86204 hypothetical protein [imported] -
Arabidopsis thaliana, partial (83%)
Length = 731
Score = 27.3 bits (59), Expect = 9.3
Identities = 21/73 (28%), Positives = 32/73 (43%)
Frame = -2
Query: 28 VKWWKLFTPDQCSGINKFVANFSIPLLSFQVISSNNVYKMSLKLIYADCIQKLLAILALT 87
+KW L D +G + F + + SNNV S ++ IQ+ L ++ L
Sbjct: 229 IKWEVLHLSDCITGSSYFTKTNPCLSSKLRCLQSNNVQNSS--ILRE*AIQRSL*LVFLD 56
Query: 88 LIIKISARGGLKW 100
L I+I GL W
Sbjct: 55 LFIEILNVNGLIW 17
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.325 0.140 0.410
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,835,043
Number of Sequences: 36976
Number of extensions: 145275
Number of successful extensions: 1094
Number of sequences better than 10.0: 33
Number of HSP's better than 10.0 without gapping: 1080
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1092
length of query: 359
length of database: 9,014,727
effective HSP length: 97
effective length of query: 262
effective length of database: 5,428,055
effective search space: 1422150410
effective search space used: 1422150410
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0391.5