BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0387.5
(124 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC88231 similar to GP|21553612|gb|AAM62705.1 unknown {Arabidopsi... 158 4e-40
BE249103 weakly similar to GP|6630450|gb| F23N19.10 {Arabidopsis... 27 2.6
TC84130 similar to GP|14326479|gb|AAK60285.1 AT5g56980/MHM17_10 ... 26 4.5
BQ144518 26 4.5
TC79915 similar to GP|17979379|gb|AAL49915.1 unknown protein {Ar... 25 5.9
TC76648 homologue to PIR|T52341|T52341 ADP-ribosylation factor [... 25 5.9
BE203221 similar to PIR|E96621|E966 hypothetical protein F23H11.... 25 5.9
BQ751862 25 5.9
AW776731 GP|19683016|g hypothetical protein {Dictyostelium disco... 25 5.9
BQ751849 similar to GP|23491586|dbj calponin {Branchiostoma belc... 25 7.7
BQ158040 25 7.7
>TC88231 similar to GP|21553612|gb|AAM62705.1 unknown {Arabidopsis
thaliana}, partial (11%)
Length = 765
Score = 158 bits (400), Expect = 4e-40
Identities = 84/126 (66%), Positives = 102/126 (80%), Gaps = 2/126 (1%)
Frame = +2
Query: 1 MEVAVELEDDLFFADLSKQLSLLIMDEDDDPLASCAVHP-QSFSGAIHPPPQSAILYEQV 59
MEVA+E+EDDLFFA+LSK+++LLIMDED+DPLAS + Q FS AIHPPPQ YEQ
Sbjct: 68 MEVALEMEDDLFFANLSKEIALLIMDEDEDPLASQPPNSLQDFSRAIHPPPQFDFFYEQA 247
Query: 60 L-RRQSKGTGVFIPQSTQPRRKQRKGRTSSYAKYQKQSQDTRMISHVPIKNPFKETNDRA 118
L RR+SKGTGVFIPQ+TQPRR+ +KGR++S++KYQKQSQDTRMIS VP KN K+
Sbjct: 248 LIRRESKGTGVFIPQATQPRRRYKKGRSNSFSKYQKQSQDTRMISQVPNKNSIKQ*K--- 418
Query: 119 YIDPSA 124
Y DPS+
Sbjct: 419 YEDPSS 436
>BE249103 weakly similar to GP|6630450|gb| F23N19.10 {Arabidopsis thaliana},
partial (20%)
Length = 613
Score = 26.6 bits (57), Expect = 2.6
Identities = 11/19 (57%), Positives = 11/19 (57%)
Frame = +3
Query: 33 ASCAVHPQSFSGAIHPPPQ 51
A CA P GAIH PPQ
Sbjct: 522 AGCAGQPHGEHGAIHEPPQ 578
>TC84130 similar to GP|14326479|gb|AAK60285.1 AT5g56980/MHM17_10
{Arabidopsis thaliana}, partial (13%)
Length = 846
Score = 25.8 bits (55), Expect = 4.5
Identities = 16/56 (28%), Positives = 24/56 (42%)
Frame = +2
Query: 34 SCAVHPQSFSGAIHPPPQSAILYEQVLRRQSKGTGVFIPQSTQPRRKQRKGRTSSY 89
S +VH + F G +HP A+ Q + Q + P+S + R K T Y
Sbjct: 161 SLSVHRRRFLGVLHPLDSVAVNLNQHQQHQQQQQRQKKPRSNRFRTKT*PSSTGQY 328
>BQ144518
Length = 1449
Score = 25.8 bits (55), Expect = 4.5
Identities = 22/71 (30%), Positives = 31/71 (42%)
Frame = +3
Query: 12 FFADLSKQLSLLIMDEDDDPLASCAVHPQSFSGAIHPPPQSAILYEQVLRRQSKGTGVFI 71
FF+ L L+I P+A C +PQ + A PP A L +VL R +G
Sbjct: 207 FFSPLPLFFPLIIPVTYCPPIALCPPNPQ-WRVAGRAPPVRAALGSRVLGRPRRGAP--- 374
Query: 72 PQSTQPRRKQR 82
P + P + R
Sbjct: 375 PDTAHPPTRPR 407
>TC79915 similar to GP|17979379|gb|AAL49915.1 unknown protein {Arabidopsis
thaliana}, partial (3%)
Length = 1090
Score = 25.4 bits (54), Expect = 5.9
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = -1
Query: 29 DDPLASCAVHPQSFSGAIHPPPQSAILYEQ 58
D P +SCA+ P+ + +IHP S + Q
Sbjct: 514 DQP*SSCALSPRDKAKSIHPRTTSTTTFLQ 425
>TC76648 homologue to PIR|T52341|T52341 ADP-ribosylation factor [imported] -
rice, complete
Length = 1357
Score = 25.4 bits (54), Expect = 5.9
Identities = 12/20 (60%), Positives = 12/20 (60%)
Frame = -2
Query: 37 VHPQSFSGAIHPPPQSAILY 56
VHP S S A HP PQ LY
Sbjct: 855 VHPHSTSCATHPLPQPPGLY 796
>BE203221 similar to PIR|E96621|E966 hypothetical protein F23H11.8 [imported]
- Arabidopsis thaliana, partial (5%)
Length = 309
Score = 25.4 bits (54), Expect = 5.9
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +2
Query: 13 FADLSKQLSLLIMDEDDDPLASCAVHP 39
+ D +K +SLLI+D LAS ++ P
Sbjct: 155 YKDAAKTMSLLILDTASSNLASSSISP 235
>BQ751862
Length = 481
Score = 25.4 bits (54), Expect = 5.9
Identities = 11/17 (64%), Positives = 12/17 (69%)
Frame = -3
Query: 72 PQSTQPRRKQRKGRTSS 88
PQST PR+ RK TSS
Sbjct: 413 PQSTSPRKASRKRSTSS 363
>AW776731 GP|19683016|g hypothetical protein {Dictyostelium discoideum},
partial (1%)
Length = 413
Score = 25.4 bits (54), Expect = 5.9
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = +2
Query: 60 LRRQSKGTGVFIPQSTQPRRKQ 81
LRR S + IP ST+PR +Q
Sbjct: 233 LRRSSTSRSLLIPFSTKPRHRQ 298
>BQ751849 similar to GP|23491586|dbj calponin {Branchiostoma belcheri},
partial (16%)
Length = 684
Score = 25.0 bits (53), Expect = 7.7
Identities = 14/44 (31%), Positives = 22/44 (49%), Gaps = 1/44 (2%)
Frame = +1
Query: 39 PQSFSGAIHPPPQSAIL-YEQVLRRQSKGTGVFIPQSTQPRRKQ 81
P S A PP++ L + +++R + G +P QPRR Q
Sbjct: 265 PASLQTATSKPPRTRRLPHGRLIRAKGSRPGAPVPGRLQPRRPQ 396
>BQ158040
Length = 1017
Score = 25.0 bits (53), Expect = 7.7
Identities = 17/56 (30%), Positives = 24/56 (42%), Gaps = 3/56 (5%)
Frame = +3
Query: 30 DPLASCAVHPQSFSGAIHPPP---QSAILYEQVLRRQSKGTGVFIPQSTQPRRKQR 82
DP A C P +G PPP ++ + R +G F+P PRRK +
Sbjct: 24 DPKAGCTTRP---TGEAAPPPPMRSHNSIHISLDPRTEQGKKPFVPA*GIPRRKPK 182
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.316 0.131 0.371
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,352,069
Number of Sequences: 36976
Number of extensions: 41000
Number of successful extensions: 244
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 240
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 242
length of query: 124
length of database: 9,014,727
effective HSP length: 84
effective length of query: 40
effective length of database: 5,908,743
effective search space: 236349720
effective search space used: 236349720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)
Lotus: description of TM0387.5