BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0383.7
(316 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC77695 similar to PIR|T09704|T09704 probable arginine/serine-ri... 49 3e-06
TC92312 weakly similar to GP|4567275|gb|AAD23688.1| expressed pr... 48 6e-06
TC77694 homologue to PIR|T09704|T09704 probable arginine/serine-... 47 7e-06
TC87506 similar to GP|9843655|emb|CAC03601.1 SC35-like splicing ... 43 1e-04
TC84935 similar to PIR|G96631|G96631 probable RNA-binding protei... 43 2e-04
TC77388 similar to PIR|E96553|E96553 probable RNA-binding protei... 42 2e-04
TC88921 similar to SP|P92966|RS41_ARATH Arginine/serine-rich spl... 42 4e-04
BI266384 40 0.002
TC87868 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 39 0.002
TC90571 similar to GP|15594035|emb|CAC69852. nucleic acid bindin... 39 0.003
TC76334 similar to GP|9663767|emb|CAC01237.1 RNA Binding Protein... 39 0.003
TC78771 similar to GP|16612268|gb|AAL27502.1 AT3g61860/F21F14_30... 38 0.004
AL368496 similar to GP|16612268|gb| AT3g61860/F21F14_30 {Arabido... 38 0.006
BG449291 similar to GP|2204089|emb| FCA gamma {Arabidopsis thali... 37 0.008
TC76468 similar to PIR|E84638|E84638 probable RSZp22 splicing fa... 37 0.008
TC79394 weakly similar to PIR|T49019|T49019 probable RNA binding... 37 0.008
AJ388976 similar to PIR|E84638|E84 probable RSZp22 splicing fact... 37 0.008
TC83750 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 37 0.008
TC83666 similar to GP|15042832|gb|AAK82455.1 putative RNA bindin... 37 0.013
AL371197 homologue to GP|6273331|gb| glycine-rich RNA binding pr... 36 0.017
>TC77695 similar to PIR|T09704|T09704 probable arginine/serine-rich splicing
factor - alfalfa, partial (83%)
Length = 1657
Score = 48.5 bits (114), Expect = 3e-06
Identities = 23/79 (29%), Positives = 44/79 (55%)
Frame = +1
Query: 35 FTIFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEAIR 94
+++ V ++ + DL +F++YG + +F+ R R+ G FAF+R+KY +A +A+
Sbjct: 112 YSLLVLNITFRTTADDLFPLFDKYGKVVDIFIPRDRRTGESRGFAFVRYKYADEASKAVD 291
Query: 95 SLNGSWFFESFLMVNRARY 113
L+G + V A+Y
Sbjct: 292 RLDGRMVDGREITVQFAKY 348
>TC92312 weakly similar to GP|4567275|gb|AAD23688.1| expressed protein
{Arabidopsis thaliana}, partial (29%)
Length = 1319
Score = 47.8 bits (112), Expect = 6e-06
Identities = 28/94 (29%), Positives = 45/94 (47%)
Frame = +1
Query: 18 VGSHGVDRQQWRTDKKVFTIFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSR 77
+G G Q+W+ + V + +++R F G + VF+ ++ G
Sbjct: 244 LGGEGAKTQKWK-------LIVRNLPFKAKENEIRDAFSSAGTVWEVFIPQKSDTGLSKG 402
Query: 78 FAFIRFKYEQDACEAIRSLNGSWFFESFLMVNRA 111
FAF++F +QDA AIR LNGS F + V+ A
Sbjct: 403 FAFVKFTCKQDAENAIRKLNGSKFGSRLIAVDWA 504
>TC77694 homologue to PIR|T09704|T09704 probable arginine/serine-rich
splicing factor - alfalfa, partial (95%)
Length = 1494
Score = 47.4 bits (111), Expect = 7e-06
Identities = 22/79 (27%), Positives = 44/79 (54%)
Frame = +3
Query: 35 FTIFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEAIR 94
+++ V ++ + DL +F++YG + +F+ + R+ G FAF+R+KY +A +A+
Sbjct: 84 YSLLVLNITFRTTADDLFPLFDKYGKVVDIFIPKDRRTGESRGFAFVRYKYADEASKAVD 263
Query: 95 SLNGSWFFESFLMVNRARY 113
L+G + V A+Y
Sbjct: 264 RLDGRMVDGREITVQFAKY 320
>TC87506 similar to GP|9843655|emb|CAC03601.1 SC35-like splicing factor
SCL28 28 kD {Arabidopsis thaliana}, partial (51%)
Length = 1558
Score = 43.1 bits (100), Expect = 1e-04
Identities = 20/48 (41%), Positives = 29/48 (59%)
Frame = +3
Query: 50 DLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEAIRSLN 97
DLR FERYG + V+L R G F F+++++ +DA EA + LN
Sbjct: 450 DLRGPFERYGPVKDVYLPRNYYTGEPRGFGFVKYRHGEDAAEAKQQLN 593
>TC84935 similar to PIR|G96631|G96631 probable RNA-binding protein F8A5.17
[imported] - Arabidopsis thaliana, partial (41%)
Length = 552
Score = 42.7 bits (99), Expect = 2e-04
Identities = 28/98 (28%), Positives = 48/98 (48%)
Frame = +2
Query: 15 PEVVGSHGVDRQQWRTDKKVFTIFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGR 74
P+ V S G + + + K+ IFV G+ +V+ L F+RYG + + R GR
Sbjct: 80 PKTVVS-GFAKLEAMSGKEENRIFVGGLGWDVTERQLEHAFDRYGKILECQIMMERDTGR 256
Query: 75 KSRFAFIRFKYEQDACEAIRSLNGSWFFESFLMVNRAR 112
F FI F + +AI+ ++G + + VN+A+
Sbjct: 257 PRGFGFITFSDRRGMEDAIKEMDGREIGDRIISVNKAQ 370
>TC77388 similar to PIR|E96553|E96553 probable RNA-binding protein
35994-37391 [imported] - Arabidopsis thaliana, partial
(64%)
Length = 1029
Score = 42.4 bits (98), Expect = 2e-04
Identities = 29/95 (30%), Positives = 44/95 (45%), Gaps = 4/95 (4%)
Frame = +2
Query: 37 IFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEAIRSL 96
I V GV + DL+ F YG + + L R+ G +A I ++ ++A AI +L
Sbjct: 335 ILVTGVHEEAQDDDLQNAFGEYGDIKNLHLNLDRRTGFVKGYALIEYERSEEARNAIENL 514
Query: 97 NGSWFFESFLMVNRA----RYDYTNRRNVTNRHSK 127
NGS + V+ A + T RRN R S+
Sbjct: 515 NGSVLLTQTIYVDWAFSSGPINETVRRNRPQRRSR 619
>TC88921 similar to SP|P92966|RS41_ARATH Arginine/serine-rich splicing
factor RSP41. [Mouse-ear cress] {Arabidopsis thaliana},
partial (33%)
Length = 1419
Score = 41.6 bits (96), Expect = 4e-04
Identities = 29/92 (31%), Positives = 44/92 (47%)
Frame = +1
Query: 29 RTDKKVFTIFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQD 88
R K +F I D DL R FE YG + V +++ FAF++++ E D
Sbjct: 70 RPSKTLFVINFDTYQTRT--RDLERHFEPYGKIVSVRIRKN--------FAFVQYESEDD 219
Query: 89 ACEAIRSLNGSWFFESFLMVNRARYDYTNRRN 120
AC+A+ + N S + + V A D +RRN
Sbjct: 220 ACKALEATNNSKLMDRVISVEFAARD-DDRRN 312
>BI266384
Length = 626
Score = 39.7 bits (91), Expect = 0.002
Identities = 23/76 (30%), Positives = 39/76 (51%)
Frame = +2
Query: 37 IFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEAIRSL 96
IF+ G+ + + L ++F +G + V L +K G+ FA+I F E A A + +
Sbjct: 215 IFIKGLPLSTTELHLTKVFSMFGEVTQVKLLIDKKTGQSLGFAYIWFVEEDSAQSAAKEM 394
Query: 97 NGSWFFESFLMVNRAR 112
NG F+ F+ V A+
Sbjct: 395 NGKLFYGRFIYVTIAK 442
>TC87868 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana, partial
(91%)
Length = 860
Score = 39.3 bits (90), Expect = 0.002
Identities = 23/69 (33%), Positives = 37/69 (53%)
Frame = +3
Query: 30 TDKKVFTIFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDA 89
T++ + ++V + VS DL F +GV+ V++ RR +AFI F +DA
Sbjct: 30 TNETMSRVYVGNLDSRVSERDLEDEFRVFGVIRSVWVARR-----PPGYAFIDFDDRRDA 194
Query: 90 CEAIRSLNG 98
+AIR L+G
Sbjct: 195QDAIRDLDG 221
>TC90571 similar to GP|15594035|emb|CAC69852. nucleic acid binding protein
{Nicotiana tabacum}, partial (26%)
Length = 871
Score = 38.9 bits (89), Expect = 0.003
Identities = 25/80 (31%), Positives = 41/80 (51%)
Frame = +2
Query: 20 SHGVDRQQWRTDKKVFTIFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFA 79
S+G Q D TIFV G+ NV+ DL++ F +YG + V + + G
Sbjct: 128 SNGTSSQS-EADSTNTTIFVGGLDSNVTDEDLKQTFSQYGEIASVKIPVGKGCG------ 286
Query: 80 FIRFKYEQDACEAIRSLNGS 99
F++F ++ EA++ LNG+
Sbjct: 287 FVQFANRNNSEEALQKLNGT 346
>TC76334 similar to GP|9663767|emb|CAC01237.1 RNA Binding Protein 45
{Nicotiana plumbaginifolia}, partial (68%)
Length = 1579
Score = 38.9 bits (89), Expect = 0.003
Identities = 28/89 (31%), Positives = 42/89 (46%)
Frame = +1
Query: 11 PVSRPEVVGSHGVDRQQWRTDKKVFTIFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRR 70
P + + S+ Q D TIFV + NV+ LR++F +YG L V + +
Sbjct: 760 PAATTQPKASYNPSGGQSENDPNNTTIFVGNLDPNVTDDHLRQVFTQYGELVHVKIPSGK 939
Query: 71 KQGRKSRFAFIRFKYEQDACEAIRSLNGS 99
+ G F++F A EAIR LNG+
Sbjct: 940 RCG------FVQFSDRSCAEEAIRVLNGT 1008
>TC78771 similar to GP|16612268|gb|AAL27502.1 AT3g61860/F21F14_30
{Arabidopsis thaliana}, partial (87%)
Length = 1254
Score = 38.1 bits (87), Expect = 0.004
Identities = 31/104 (29%), Positives = 51/104 (48%), Gaps = 2/104 (1%)
Frame = +1
Query: 33 KVFTIFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEA 92
K+ +F + + +L R+F +YG + V + KS FAF+ ++ E+DA EA
Sbjct: 85 KMRPVFAGNLEYDTRQSELERLFSKYGRIERVDM--------KSGFAFVYYEDERDAEEA 240
Query: 93 IRSLNGSWF--FESFLMVNRARYDYTNRRNVTNRHSKVWKQKFV 134
IR+L+ F + L V AR + R+ + + K K FV
Sbjct: 241 IRALDNIPFGHDKRRLSVEWARGERGRHRDGSKPNQKPTKTLFV 372
>AL368496 similar to GP|16612268|gb| AT3g61860/F21F14_30 {Arabidopsis
thaliana}, partial (36%)
Length = 616
Score = 37.7 bits (86), Expect = 0.006
Identities = 32/102 (31%), Positives = 51/102 (49%), Gaps = 6/102 (5%)
Frame = -2
Query: 39 VDGVSDNVSYH----DLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEAIR 94
+ G + N+ Y +L R+F +YG + V + KS FAF+ ++ E+DA EAIR
Sbjct: 603 IGGFAGNLEYDTRQSELERLFSKYGRIERVDM--------KSGFAFVYYEDERDAEEAIR 448
Query: 95 SLNGSWF--FESFLMVNRARYDYTNRRNVTNRHSKVWKQKFV 134
+L+ F + L V AR + R+ + + K K FV
Sbjct: 447 ALDNIPFGHDKRRLSVEWARGERGRHRDGSKPNQKPTKTLFV 322
>BG449291 similar to GP|2204089|emb| FCA gamma {Arabidopsis thaliana},
partial (9%)
Length = 593
Score = 37.4 bits (85), Expect = 0.008
Identities = 20/61 (32%), Positives = 34/61 (54%)
Frame = +2
Query: 37 IFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEAIRSL 96
+FV V + D+R +FE +G + V L + RK G+ FI++ +A +AIR+L
Sbjct: 389 LFVGSVPRTATEEDIRPLFEEHGNVVEVALIKDRKTGQHQGCCFIKYATSXEADQAIRAL 568
Query: 97 N 97
+
Sbjct: 569 H 571
>TC76468 similar to PIR|E84638|E84638 probable RSZp22 splicing factor
[imported] - Arabidopsis thaliana, partial (39%)
Length = 465
Score = 37.4 bits (85), Expect = 0.008
Identities = 20/62 (32%), Positives = 33/62 (52%)
Frame = +3
Query: 37 IFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEAIRSL 96
+++ + +S DL F +GV+ V++ RR +AFI F +DA +AIR L
Sbjct: 177 VYIGNLDSRISERDLEDDFHVFGVIRSVWVARR-----PPGYAFIDFDDRRDALDAIREL 341
Query: 97 NG 98
+G
Sbjct: 342 DG 347
>TC79394 weakly similar to PIR|T49019|T49019 probable RNA binding protein -
Arabidopsis thaliana, partial (74%)
Length = 1909
Score = 37.4 bits (85), Expect = 0.008
Identities = 16/66 (24%), Positives = 36/66 (54%)
Frame = +2
Query: 30 TDKKVFTIFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDA 89
+ +V ++V + +N++ + L+ +FE +G + V L + KSR+ F+ F A
Sbjct: 938 SSSQVKAVYVKNLPENITQNRLKELFEHHGKITKVALPPAKAGQEKSRYGFVHFADRSSA 1117
Query: 90 CEAIRS 95
+A+++
Sbjct: 1118 MKALKN 1135
>AJ388976 similar to PIR|E84638|E84 probable RSZp22 splicing factor
[imported] - Arabidopsis thaliana, partial (62%)
Length = 508
Score = 37.4 bits (85), Expect = 0.008
Identities = 20/62 (32%), Positives = 33/62 (52%)
Frame = +2
Query: 37 IFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEAIRSL 96
+++ + +S DL F +GV+ V++ RR +AFI F +DA +AIR L
Sbjct: 47 VYIGNLDSRISERDLEDDFHVFGVIRSVWVARR-----PPGYAFIDFDDRRDALDAIREL 211
Query: 97 NG 98
+G
Sbjct: 212 DG 217
>TC83750 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana, partial (68%)
Length = 772
Score = 37.4 bits (85), Expect = 0.008
Identities = 20/62 (32%), Positives = 33/62 (52%)
Frame = +2
Query: 37 IFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEAIRSL 96
+++ + +S DL F +GV+ V++ RR +AFI F +DA +AIR L
Sbjct: 53 VYIGNLDSRISERDLEDDFHVFGVIRSVWVARR-----PPGYAFIDFDDRRDALDAIREL 217
Query: 97 NG 98
+G
Sbjct: 218 DG 223
>TC83666 similar to GP|15042832|gb|AAK82455.1 putative RNA binding protein
{Oryza sativa}, partial (52%)
Length = 896
Score = 36.6 bits (83), Expect = 0.013
Identities = 21/73 (28%), Positives = 39/73 (52%), Gaps = 1/73 (1%)
Frame = +1
Query: 28 WRTDKKVFT-IFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYE 86
W T K +FV G+ + + D+ +F +YG + + L R + G+ FAFI ++ +
Sbjct: 244 WHTKYKDSAYVFVGGIPFDFTEGDVIAVFAQYGEVVDINLVRDKGTGKSKGFAFIAYEDQ 423
Query: 87 QDACEAIRSLNGS 99
+ A+ +LNG+
Sbjct: 424 RSTNLAVDNLNGA 462
>AL371197 homologue to GP|6273331|gb| glycine-rich RNA binding protein
{Medicago sativa}, complete
Length = 437
Score = 36.2 bits (82), Expect = 0.017
Identities = 21/78 (26%), Positives = 37/78 (46%)
Frame = +1
Query: 35 FTIFVDGVSDNVSYHDLRRMFERYGVLHGVFLQRRRKQGRKSRFAFIRFKYEQDACEAIR 94
+ FV G++ L + F +YG + + R+ GR F F+ F E+ +AI
Sbjct: 58 YRCFVGGLAWATDNEALEKAFSQYGEIVDSKIINDRETGRSRGFGFVTFANEKSMNDAIE 237
Query: 95 SLNGSWFFESFLMVNRAR 112
++NG + VN+A+
Sbjct: 238 AMNGQDLDGRNITVNQAQ 291
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.323 0.137 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,570,843
Number of Sequences: 36976
Number of extensions: 121557
Number of successful extensions: 813
Number of sequences better than 10.0: 75
Number of HSP's better than 10.0 without gapping: 801
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 811
length of query: 316
length of database: 9,014,727
effective HSP length: 96
effective length of query: 220
effective length of database: 5,465,031
effective search space: 1202306820
effective search space used: 1202306820
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0383.7