BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0380.6
(347 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC81021 similar to PIR|T06123|T06123 UDP-galactose transport pro... 309 e-132
TC86708 similar to GP|21553407|gb|AAM62500.1 unknown {Arabidopsi... 77 1e-14
TC80663 similar to GP|21740671|emb|CAD41134. OSJNBa0084K20.14 {O... 72 2e-13
TC91728 similar to PIR|T06123|T06123 UDP-galactose transport pro... 47 1e-05
BF632492 similar to GP|14532830|gb putative transporter protein ... 35 0.056
AW691185 31 0.62
TC87737 31 0.81
BG455075 similar to GP|5139541|emb nodulin26-like major intrinsi... 30 1.8
CA989271 similar to GP|11034569|db hypothetical protein~similar ... 29 2.3
TC93057 homologue to GP|23194453|gb|AAN15183.1 MADS box protein ... 28 5.2
BQ144379 homologue to GP|1597741|gb|A unknown {Azospirillum bras... 28 5.2
TC84438 homologue to GP|1483232|emb|CAA67969.1 MADS5 protein {Be... 28 6.8
TC78819 similar to GP|9558421|dbj|BAB03357.1 ESTs AU097577(C6200... 28 6.8
TC91330 weakly similar to PIR|A84793|A84793 nodulin-like protein... 28 6.8
TC85931 similar to GP|10177452|dbj|BAB10843. hypersensitive-indu... 27 8.9
>TC81021 similar to PIR|T06123|T06123 UDP-galactose transport protein
homolog F23E12.100 - Arabidopsis thaliana, partial (66%)
Length = 873
Score = 309 bits (792), Expect(3) = e-132
Identities = 151/168 (89%), Positives = 159/168 (93%)
Frame = +1
Query: 99 WNKEGVTDDNRLTTTLDEVIVYPIPAALYLVKNLLQYYIFAYVDAPGYQILKNFNIISTG 158
W EGVT+ N+LT +L EVIVYPIPAALYL KNLLQYYIFAYVDAPGYQILKN NII+TG
Sbjct: 229 WRTEGVTEQNKLTASLKEVIVYPIPAALYLFKNLLQYYIFAYVDAPGYQILKNLNIITTG 408
Query: 159 VLYRIILKKRLSEIQWAAFILLTAGCTTAQLNSNSDHVLQTPFQGWVMAIAMALLSGFAG 218
VLYRIILKK+LSEIQWAAF+LLTAGCTTAQLNSNSDHVLQTP QGWVMAI MALLSGFAG
Sbjct: 409 VLYRIILKKKLSEIQWAAFVLLTAGCTTAQLNSNSDHVLQTPVQGWVMAIVMALLSGFAG 588
Query: 219 VYTEAIIKKRPSRNINVQNFWLYVFGMGFNAVAILVQDFDAVMNKGFF 266
VYTEAIIKKRPS+NINVQNFWLYVFGMGFNAVAILVQDFDAV+NKGFF
Sbjct: 589 VYTEAIIKKRPSQNINVQNFWLYVFGMGFNAVAILVQDFDAVVNKGFF 732
Score = 115 bits (287), Expect(3) = e-132
Identities = 56/69 (81%), Positives = 64/69 (92%)
Frame = +3
Query: 27 SDGETKVDSHRTQVQWKRKSMVTFALTILTSSQAILIVWSKRAGKYEYSVTTANFMVETL 86
S GE K+D +R + QWKRKS+VTFALTILTSSQAILIVWSKRAGKY+YSVTTANF+VETL
Sbjct: 12 SIGEPKIDINRIKPQWKRKSLVTFALTILTSSQAILIVWSKRAGKYDYSVTTANFLVETL 191
Query: 87 KCAISLVAL 95
KCAIS++AL
Sbjct: 192KCAISIIAL 218
Score = 86.3 bits (212), Expect(3) = e-132
Identities = 44/46 (95%), Positives = 44/46 (95%)
Frame = +2
Query: 267 HGYSFITVLMIFNHALSGIAVSMVMKYADNIVKVYSTSVAMLLTAV 312
HGYSFIT LMIFNHALSGIAVSMVMKYADNI KVYSTSVAMLLTAV
Sbjct: 734 HGYSFITFLMIFNHALSGIAVSMVMKYADNIGKVYSTSVAMLLTAV 871
>TC86708 similar to GP|21553407|gb|AAM62500.1 unknown {Arabidopsis
thaliana}, partial (93%)
Length = 1446
Score = 76.6 bits (187), Expect = 1e-14
Identities = 80/320 (25%), Positives = 142/320 (44%), Gaps = 19/320 (5%)
Frame = +3
Query: 38 TQVQWKRKSMVTFALTILTSSQAILIVWSKRAGKYEYSVTTANFMVETLKCAISLV---- 93
TQ Q ++ F++ + A ++ SKR E VT++ E K +L
Sbjct: 165 TQAQGNSARILFFSVLLALQYGAQPLI-SKRFISREVIVTSSVLSCEAAKVVFALYFMAK 341
Query: 94 --ALGRIWNKEGVTDDNRLTTTLDEVIVYPIPAALYLVKNLLQYYIFAYVDAPGYQILKN 151
++GR++ + T + + +PAA+Y ++N L + +D+ + IL
Sbjct: 342 EGSMGRMYKE---------WTLVGALTASGLPAAIYALQNSLLQISYKNLDSLTFSILNQ 494
Query: 152 FNIISTGVLYRIILKKRLSEIQWAAFILLTA---------GCTTAQLNSNSDHVLQTPFQ 202
I T + +L+++ S Q A LL A G +N+D VL F
Sbjct: 495 TKIFFTALFTYFMLRQKQSIQQMGALFLLIAAAVLLSVGEGSNKGSTGANADQVL---FY 665
Query: 203 GWVMAIAMALLSGFAGVYTE--AIIKKRPSRNINVQNFWLYVFGMGFNAVAILVQDFDAV 260
G + + ++LSG A + + +KK S + V+ V + A + D +A+
Sbjct: 666 GIIPVLIASVLSGLASSLCQWASQVKKHSSYLMTVE--MSIVGSICLIASTLKSPDGEAM 839
Query: 261 MNKGFFHGYSFITVLMIFNHALSGIAVSMVMKYADNIVKVYSTSVAMLLTAVVSVFLFGF 320
GFFHG++ +T + + ++AL GI V +V YA + K + A+L+TA++ G
Sbjct: 840 RRHGFFHGWTPLTWIPVISNALGGILVGLVTSYAGGVRKGFVIVSALLVTALLQFIFEGK 1019
Query: 321 HLSL--AFFLGTIVVSVAIY 338
SL L + S++IY
Sbjct: 1020PPSLYCLVALPLVASSISIY 1079
>TC80663 similar to GP|21740671|emb|CAD41134. OSJNBa0084K20.14 {Oryza
sativa}, partial (53%)
Length = 900
Score = 72.4 bits (176), Expect = 2e-13
Identities = 52/191 (27%), Positives = 90/191 (46%), Gaps = 8/191 (4%)
Frame = +1
Query: 9 EDKVRDSDVESVAVNNTVSDGETKVDSHRTQVQWKRKSMVTFAL---TILTSSQAILIVW 65
E V S + S A TVS D H+ ++ K++ + + +L Q IL+
Sbjct: 247 ECSVCHSKLVSPAATKTVSRA---YDRHKNRITSKQRVLNILLVGGDCMLVGFQPILVYM 417
Query: 66 SKRAGKYEYSVTTANFMVETLKCAISLVALGRIWNKEGVTDDNRLTTT-----LDEVIVY 120
SK GK+ +S + NF+ E K ++V L + V D L+ + ++
Sbjct: 418 SKVDGKFNFSPISVNFLTEITKVFFAIVMLLLQARNQKVGDKPLLSISTFMQAARNNVLL 597
Query: 121 PIPAALYLVKNLLQYYIFAYVDAPGYQILKNFNIISTGVLYRIILKKRLSEIQWAAFILL 180
+PA LY + N L++ + Y + ++L N ++ +L ++++K+R S IQW A LL
Sbjct: 598 AVPAFLYAINNYLKFVMQLYFNPATVKMLSNLKVLVIALLLKVVMKRRFSIIQWEALALL 777
Query: 181 TAGCTTAQLNS 191
G + QL S
Sbjct: 778 LIGISVNQLRS 810
>TC91728 similar to PIR|T06123|T06123 UDP-galactose transport protein
homolog F23E12.100 - Arabidopsis thaliana, partial (21%)
Length = 302
Score = 47.0 bits (110), Expect = 1e-05
Identities = 23/25 (92%), Positives = 24/25 (96%)
Frame = +3
Query: 323 SLAFFLGTIVVSVAIYLHSAGKMQR 347
SLAFFLGTI VSV+IYLHSAGKMQR
Sbjct: 3 SLAFFLGTIGVSVSIYLHSAGKMQR 77
>BF632492 similar to GP|14532830|gb putative transporter protein {Arabidopsis
thaliana}, partial (29%)
Length = 658
Score = 34.7 bits (78), Expect = 0.056
Identities = 26/90 (28%), Positives = 42/90 (45%), Gaps = 3/90 (3%)
Frame = +2
Query: 9 EDKVRDSDVESVAVNNTVSDGETKVDSHRTQVQWKRKSMVTFAL---TILTSSQAILIVW 65
E V S + S A TVS D H+ ++ K++ + + +L Q IL+
Sbjct: 266 ECSVCHSKLVSPAATKTVSRA---YDRHKNRITSKQRVLNILLVGGDCMLVGFQPILVYM 436
Query: 66 SKRAGKYEYSVTTANFMVETLKCAISLVAL 95
SK GK+ +S + NF+ E K ++V L
Sbjct: 437 SKVDGKFNFSPISVNFLTEITKVFFAIVML 526
>AW691185
Length = 540
Score = 31.2 bits (69), Expect = 0.62
Identities = 16/54 (29%), Positives = 28/54 (51%)
Frame = +2
Query: 292 KYADNIVKVYSTSVAMLLTAVVSVFLFGFHLSLAFFLGTIVVSVAIYLHSAGKM 345
K+ D + V V + T + F + L+FFL ++VSV+++L+ KM
Sbjct: 161 KHVDQLTNVRLNVVTPVRTKNLPFSCVNF*VLLSFFLSKVIVSVSLFLNKPSKM 322
>TC87737
Length = 491
Score = 30.8 bits (68), Expect = 0.81
Identities = 18/37 (48%), Positives = 21/37 (56%)
Frame = +3
Query: 292 KYADNIVKVYSTSVAMLLTAVVSVFLFGFHLSLAFFL 328
KY NI+ Y SV+ LL +V FLF FH FFL
Sbjct: 186 KYCQNII--YCISVSKLLCSVFLFFLFFFHFVK*FFL 290
>BG455075 similar to GP|5139541|emb nodulin26-like major intrinsic protein
{Pisum sativum}, partial (59%)
Length = 652
Score = 29.6 bits (65), Expect = 1.8
Identities = 16/50 (32%), Positives = 28/50 (56%)
Frame = +3
Query: 164 ILKKRLSEIQWAAFILLTAGCTTAQLNSNSDHVLQTPFQGWVMAIAMALL 213
+L+K ++E+ F L+ AGC +N N+D V+ P V +A+ +L
Sbjct: 168 LLQKLVAEVV-GTFFLIFAGCAAVVVNLNNDKVVTLPGISIVWGLAVMVL 314
>CA989271 similar to GP|11034569|db hypothetical protein~similar to
Arabidopsis thaliana chromosome 4 F28J12.200, partial
(21%)
Length = 663
Score = 29.3 bits (64), Expect = 2.3
Identities = 14/45 (31%), Positives = 22/45 (48%)
Frame = -2
Query: 98 IWNKEGVTDDNRLTTTLDEVIVYPIPAALYLVKNLLQYYIFAYVD 142
IW+ G+ + ++L L +VI Y L KNL Y+ V+
Sbjct: 176 IWSNLGLLNGSKLHKILRQVICYSFSVCLKHCKNLFSYFCLPIVE 42
>TC93057 homologue to GP|23194453|gb|AAN15183.1 MADS box protein GHMADS-2
{Gossypium hirsutum}, partial (95%)
Length = 858
Score = 28.1 bits (61), Expect = 5.2
Identities = 14/42 (33%), Positives = 22/42 (52%)
Frame = +3
Query: 50 FALTILTSSQAILIVWSKRAGKYEYSVTTANFMVETLKCAIS 91
+ L++L ++ LIV+S R YEYS ++ K A S
Sbjct: 126 YELSVLCDAEVALIVFSSRGRLYEYSNNNIRSTIDRYKKACS 251
>BQ144379 homologue to GP|1597741|gb|A unknown {Azospirillum brasilense},
partial (5%)
Length = 1005
Score = 28.1 bits (61), Expect = 5.2
Identities = 17/50 (34%), Positives = 23/50 (46%), Gaps = 6/50 (12%)
Frame = -1
Query: 176 AFILLTAGCTTAQLNSNSDHVLQTPF------QGWVMAIAMALLSGFAGV 219
A ++ GC A LN + H+LQ PF + W + L GF GV
Sbjct: 636 AAVINQLGCGAAGLNKPTLHLLQKPFAVAAQWEIWFKNYNIISLGGFVGV 487
>TC84438 homologue to GP|1483232|emb|CAA67969.1 MADS5 protein {Betula
pendula}, partial (24%)
Length = 702
Score = 27.7 bits (60), Expect = 6.8
Identities = 23/81 (28%), Positives = 38/81 (46%), Gaps = 2/81 (2%)
Frame = +1
Query: 265 FFHGYSFITVLMIFNHALS-GIAVSMVMKYADNIVKVYSTSVAMLLTAVVSVFLFGFHLS 323
FFH Y FI +L++ ++ I +S+++ NI+ SV +L+ L+ HL
Sbjct: 418 FFHIYKFILLLLLLLFQINLIIMISLLLHLNPNII----ISV*LLIDLAEICLLYATHLY 585
Query: 324 L-AFFLGTIVVSVAIYLHSAG 343
L A FL + + H G
Sbjct: 586 LYACFL*IFYLCCQFFFHWTG 648
>TC78819 similar to GP|9558421|dbj|BAB03357.1 ESTs AU097577(C62003)
C28669(C62003) correspond to a region of the predicted
gene.~Similar to, complete
Length = 1064
Score = 27.7 bits (60), Expect = 6.8
Identities = 17/43 (39%), Positives = 21/43 (48%)
Frame = +2
Query: 166 KKRLSEIQWAAFILLTAGCTTAQLNSNSDHVLQTPFQGWVMAI 208
KKRL+EIQ AA +L T + V Q P WV+ I
Sbjct: 350 KKRLAEIQEAAKVLRYGSVTPISGSDFVREVSQAPSDVWVVVI 478
>TC91330 weakly similar to PIR|A84793|A84793 nodulin-like protein [imported]
- Arabidopsis thaliana, partial (34%)
Length = 812
Score = 27.7 bits (60), Expect = 6.8
Identities = 22/76 (28%), Positives = 34/76 (43%), Gaps = 8/76 (10%)
Frame = +3
Query: 277 IFNHALSGIAVSMVMKYADNIV-----KVYSTS---VAMLLTAVVSVFLFGFHLSLAFFL 328
+F A +GI S V Y +V V T+ V M++ ++ + L L +
Sbjct: 207 LFAPAYAGIVTSGVQYYIQGLVIKTMGPVIVTAFNPVRMIIVTALACIILSEQLFLGSII 386
Query: 329 GTIVVSVAIYLHSAGK 344
G IVV + +YL GK
Sbjct: 387 GAIVVVLGLYLVVWGK 434
>TC85931 similar to GP|10177452|dbj|BAB10843. hypersensitive-induced
response protein {Arabidopsis thaliana}, partial (71%)
Length = 779
Score = 27.3 bits (59), Expect = 8.9
Identities = 16/50 (32%), Positives = 26/50 (52%), Gaps = 3/50 (6%)
Frame = -3
Query: 75 SVTTANFMVETLKCAISLVALGRIWNKEGVTDDN---RLTTTLDEVIVYP 121
S+ + + + +KC ISLV G + N+ D + L TLD ++YP
Sbjct: 462 SLNLVSCVAKFVKCIISLVCQGTVLNRSNNIDKDIILCLCLTLDIQLLYP 313
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.325 0.137 0.396
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,741,952
Number of Sequences: 36976
Number of extensions: 123919
Number of successful extensions: 971
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 966
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 970
length of query: 347
length of database: 9,014,727
effective HSP length: 97
effective length of query: 250
effective length of database: 5,428,055
effective search space: 1357013750
effective search space used: 1357013750
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0380.6