BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0371b.6
(264 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC87156 similar to GP|19310745|gb|AAL85103.1 unknown protein {Ar... 186 6e-48
TC77420 weakly similar to GP|19310745|gb|AAL85103.1 unknown prot... 171 2e-43
TC82124 weakly similar to GP|21554115|gb|AAM63195.1 unknown {Ara... 65 3e-11
TC77801 weakly similar to GP|21536807|gb|AAM61139.1 unknown {Ara... 39 0.002
TC86679 similar to GP|17065046|gb|AAL32677.1 Unknown protein {Ar... 29 1.6
TC80288 weakly similar to PIR|T06029|T06029 hypothetical protein... 29 2.1
BI270807 similar to GP|16326135|db Myb {Nicotiana tabacum}, part... 28 2.8
TC91308 similar to GP|15450621|gb|AAK96582.1 At2g30530/T6B20.12 ... 28 3.6
BQ751130 weakly similar to GP|15289796|dbj putative monosacchari... 28 4.7
TC80931 similar to GP|14190783|gb|AAF65166.2 putative phloem tra... 28 4.7
TC80884 similar to PIR|C86255|C86255 protein F12F1.11 [imported]... 27 8.1
BF648378 similar to GP|9294677|dbj| gene_id:F14O13.28~pir||T2263... 27 8.1
>TC87156 similar to GP|19310745|gb|AAL85103.1 unknown protein {Arabidopsis
thaliana}, partial (10%)
Length = 803
Score = 186 bits (473), Expect = 6e-48
Identities = 133/252 (52%), Positives = 151/252 (59%), Gaps = 12/252 (4%)
Frame = +3
Query: 5 NDWVLLSDDGSLDGGDENQIFLGKSNPESKSDFDMNYFYCTSPKSRD-------SSTTQL 57
N+WV+LSDDG DG DE QIFL K N FD +YF CTSPKS Q+
Sbjct: 117 NEWVILSDDGFFDG-DEKQIFLDKRN---SLVFDKDYF-CTSPKSTKIIDIEPLKVPKQV 281
Query: 58 LHDDPIQFVENTEDDHVGDNSDTTSEKIKAVVVEADEQETLSVSQFFLQL-ENKFADMKM 116
H PIQF + +EKIK +E +QE SVSQ F + ENKF DMK+
Sbjct: 282 FHV-PIQFEPIIDKVPNEGLVKENTEKIKVPQIEEADQE--SVSQVFFHIKENKFVDMKL 452
Query: 117 DSPKSSP-RVLFPPPLDAGALKFEDKGEAMEVMVSPRRK-VENEMATMDCDREE-VDPTD 173
+SPKSS R LF P LDA ME+M SPR K +ENE M D +E D
Sbjct: 453 ESPKSSSGRGLFSP-LDAD----------MEMMTSPRMKNMENEKVDMYYDEKEGEDMNG 599
Query: 174 GFNFWKWSLTGVGAICSFGVAAATICVLVFGSQQK-NKFHQNHKIQFQIYTDDKRIKQVV 232
GFN WKWSLTGVGAICSFGVAAATICVL FGSQQ+ NK Q+ I+F+IYTDDK VV
Sbjct: 600 GFNLWKWSLTGVGAICSFGVAAATICVLFFGSQQRNNKLQQDQSIRFKIYTDDK----VV 767
Query: 233 QQATKLNEAIAA 244
Q TKLNEA AA
Sbjct: 768 QHTTKLNEAFAA 803
>TC77420 weakly similar to GP|19310745|gb|AAL85103.1 unknown protein
{Arabidopsis thaliana}, partial (17%)
Length = 1134
Score = 171 bits (434), Expect = 2e-43
Identities = 109/263 (41%), Positives = 153/263 (57%), Gaps = 5/263 (1%)
Frame = +2
Query: 5 NDWVLLSDDGSLDGGDENQIFLGKSNPESKSDFDMNYFYCTSPKSRDSSTTQLLHDDPIQ 64
++W +S+DG +D ++ E KS DM Y Y + P+ ++ QL+ P+Q
Sbjct: 191 DEWESISNDGYIDFNEDG---------EKKSALDMEYLYPSPPRKQN----QLV---PLQ 322
Query: 65 FVENTEDDHVGDNSDTTSEK--IKAVVVEADEQETLSVSQFFLQL-ENKFADMKMDSPKS 121
D +G+ S+ K K +E D++E +VSQ F ++ EN+ DMK++SPKS
Sbjct: 323 I---PLDLKIGNTSNDVLLKDITKNQSLEDDQEE--NVSQVFFKIKENEIGDMKIESPKS 487
Query: 122 SPRVLFPPPLDAGALKFEDKGEAMEVMVSPRRKVE-NEMATMDCDRE-EVDPTDGFNFWK 179
S + E + SPR +E ++ + D E + GFN WK
Sbjct: 488 S--------------------SSKEFITSPRMIIEKDDFGDKEEDSSWEEENNSGFNLWK 607
Query: 180 WSLTGVGAICSFGVAAATICVLVFGSQQKNKFHQNHKIQFQIYTDDKRIKQVVQQATKLN 239
WSL+GVGAIC+FGV AA+ICVL FGSQQ+ K ++ K +FQIY DDKRIKQVVQ ATKLN
Sbjct: 608 WSLSGVGAICTFGVVAASICVLFFGSQQRKKLQKDQKNRFQIYADDKRIKQVVQHATKLN 787
Query: 240 EAIAATRGIPLSRAHITYGGYYE 262
EAI+ RG+P++RAHIT GG Y+
Sbjct: 788 EAISVVRGVPITRAHITVGGNYD 856
>TC82124 weakly similar to GP|21554115|gb|AAM63195.1 unknown {Arabidopsis
thaliana}, partial (14%)
Length = 925
Score = 64.7 bits (156), Expect = 3e-11
Identities = 28/39 (71%), Positives = 34/39 (86%)
Frame = +3
Query: 226 KRIKQVVQQATKLNEAIAATRGIPLSRAHITYGGYYEAL 264
+ +KQVVQ TKLNEA AA RG+PLSRAHITYGGYY+++
Sbjct: 273 RSVKQVVQHTTKLNEAFAAVRGVPLSRAHITYGGYYDSV 389
>TC77801 weakly similar to GP|21536807|gb|AAM61139.1 unknown {Arabidopsis
thaliana}, partial (12%)
Length = 1145
Score = 38.9 bits (89), Expect = 0.002
Identities = 21/82 (25%), Positives = 37/82 (44%)
Frame = +2
Query: 171 PTDGFNFWKWSLTGVGAICSFGVAAATICVLVFGSQQKNKFHQNHKIQFQIYTDDKRIKQ 230
P + W + V S AAA +L+ G + + ++ + DDK++ Q
Sbjct: 671 PLEVLKHWVSPFSVVPIPLSVAAAAAFFGLLILGRRLYKMKRKTQTLKLNLALDDKKVSQ 850
Query: 231 VVQQATKLNEAIAATRGIPLSR 252
+ + +LNEA + R IP+ R
Sbjct: 851 LKDRVARLNEAFSVVRRIPVVR 916
>TC86679 similar to GP|17065046|gb|AAL32677.1 Unknown protein {Arabidopsis
thaliana}, partial (48%)
Length = 1445
Score = 29.3 bits (64), Expect = 1.6
Identities = 14/43 (32%), Positives = 21/43 (48%)
Frame = +1
Query: 60 DDPIQFVENTEDDHVGDNSDTTSEKIKAVVVEADEQETLSVSQ 102
DD + VE E+DHV E + V +E DEQ ++ +
Sbjct: 469 DDHVGDVEQDEEDHVAKIQQEEDESLDEVQLEEDEQLARAIQE 597
Score = 28.9 bits (63), Expect = 2.1
Identities = 21/76 (27%), Positives = 36/76 (46%), Gaps = 6/76 (7%)
Frame = +1
Query: 60 DDPIQFVENTEDDHVGDNSDTTSEKIKAVVVEADEQETLSVSQFFLQLENKFA-----DM 114
DD + +++ EDDHVGD + + + E DE S+ + L+ + + A +
Sbjct: 436 DDELCPLDDEEDDHVGDVEQDEEDHVAKIQQEEDE----SLDEVQLEEDEQLARAIQESL 603
Query: 115 KMD-SPKSSPRVLFPP 129
+D SP S +F P
Sbjct: 604 SIDSSPPSQTDSIFQP 651
>TC80288 weakly similar to PIR|T06029|T06029 hypothetical protein T28I19.100 -
Arabidopsis thaliana, partial (14%)
Length = 1460
Score = 28.9 bits (63), Expect = 2.1
Identities = 23/89 (25%), Positives = 39/89 (42%), Gaps = 1/89 (1%)
Frame = +3
Query: 14 GSLDGGDENQIFLGKSNPESKSDFDMNYFYCTSPKSRDSSTTQLLHDDPIQFVENTEDDH 73
G G D K+ P+S+ D N + S +SS T
Sbjct: 1038 GKETGNDSFSNETAKTKPDSQLDLSSNLTAVITEASSNSSGT------------------ 1163
Query: 74 VGDNSDTTSEKIKAVVV-EADEQETLSVS 101
GD++ ++SE+IKAV++ E+D + +V+
Sbjct: 1164 -GDDTSSSSEQIKAVILSESDHAQNATVN 1247
>BI270807 similar to GP|16326135|db Myb {Nicotiana tabacum}, partial (5%)
Length = 660
Score = 28.5 bits (62), Expect = 2.8
Identities = 22/91 (24%), Positives = 37/91 (40%), Gaps = 1/91 (1%)
Frame = +2
Query: 70 EDDHVGDNSDTTSEKIKAVVVEADEQETLSVSQFFLQLENKFADMKMDSPKSSPRVLFPP 129
E H D + T + V D+ ET + EN+ ++ +S + P
Sbjct: 203 EKKHEIDMTSTLIRNFSRLDVMFDDNETQGIEH---DKENRGPAFMVEDKSNSEEKIEQP 373
Query: 130 PLDAGALKFEDKGEAMEVMVSPRR-KVENEM 159
PLDA + D E++ P R +E++M
Sbjct: 374 PLDADSKMKNDIDTTAEIVQQPSRVLIEHDM 466
>TC91308 similar to GP|15450621|gb|AAK96582.1 At2g30530/T6B20.12
{Arabidopsis thaliana}, partial (12%)
Length = 772
Score = 28.1 bits (61), Expect = 3.6
Identities = 29/117 (24%), Positives = 51/117 (42%), Gaps = 5/117 (4%)
Frame = +1
Query: 28 KSNPESKS-DFDMNYF---YCTSPKSRDSSTTQLLHD-DPIQFVENTEDDHVGDNSDTTS 82
+S+PE + DF M Y Y TS S + + + +PI ++ +D V D S + S
Sbjct: 88 RSDPEFEEVDFSMAYRRRQYGTSKSSPLIFEDEAIRNPNPIAVSSSSSNDGV-DASSSDS 264
Query: 83 EKIKAVVVEADEQETLSVSQFFLQLENKFADMKMDSPKSSPRVLFPPPLDAGALKFE 139
KA+ + +++ L + + + SP+S P PPP + +E
Sbjct: 265 LAAKAIRASSARRDS--------SLSSLYGHSNLSSPRSQPTPTAPPPSSKDSRAYE 411
>BQ751130 weakly similar to GP|15289796|dbj putative monosaccharide
transporter 3 {Oryza sativa (japonica cultivar-group)},
partial (7%)
Length = 795
Score = 27.7 bits (60), Expect = 4.7
Identities = 23/88 (26%), Positives = 37/88 (41%), Gaps = 11/88 (12%)
Frame = -2
Query: 18 GGDENQIFLGKSNPESKSDFDMNYFYCTSPKSRDSST----TQLLHDDPIQF-------V 66
GGDE + L N SK D D+ + + KSR+ S+ + LL ++P +
Sbjct: 770 GGDELALVLLVGNDLSKLDLDVLGLFGLAAKSRECSSSIGDSALLDEEPGRLRKQKQTTA 591
Query: 67 ENTEDDHVGDNSDTTSEKIKAVVVEADE 94
EN + N D + V+ D+
Sbjct: 590 ENQGPGELDGNGDAVDTGVATVLCGIDD 507
>TC80931 similar to GP|14190783|gb|AAF65166.2 putative phloem transcription
factor M1 {Apium graveolens}, partial (67%)
Length = 1193
Score = 27.7 bits (60), Expect = 4.7
Identities = 12/27 (44%), Positives = 17/27 (62%), Gaps = 3/27 (11%)
Frame = +3
Query: 177 FWKWSLTGVGAICSFGVA---AATICV 200
++ WS G A+CS+ +A ATICV
Sbjct: 330 WFSWSAAGKEAVCSYSIAITDVATICV 410
>TC80884 similar to PIR|C86255|C86255 protein F12F1.11 [imported] -
Arabidopsis thaliana, partial (33%)
Length = 850
Score = 26.9 bits (58), Expect = 8.1
Identities = 21/96 (21%), Positives = 37/96 (37%)
Frame = +3
Query: 1 MDTTNDWVLLSDDGSLDGGDENQIFLGKSNPESKSDFDMNYFYCTSPKSRDSSTTQLLHD 60
+D +W++ S G G GK +K F+ K+ +L++
Sbjct: 366 IDAFEEWLVQSGKGGKKGK-------GKKEKRNKKGFENK----NKNKNSGELNRSVLNE 512
Query: 61 DPIQFVENTEDDHVGDNSDTTSEKIKAVVVEADEQE 96
+ E + +NS + K VVV ADE++
Sbjct: 513 STTELESVGESSNSSNNSGGSENSEKVVVVVADEEK 620
>BF648378 similar to GP|9294677|dbj| gene_id:F14O13.28~pir||T22638~similar to
unknown protein {Arabidopsis thaliana}, partial (8%)
Length = 651
Score = 26.9 bits (58), Expect = 8.1
Identities = 21/63 (33%), Positives = 29/63 (45%), Gaps = 4/63 (6%)
Frame = +2
Query: 28 KSNPESKSDFDMNYFYCTSPKSRDSSTTQLLHDDPI---QFVENTEDDHVG-DNSDTTSE 83
+S ES SD D+N F S K + ++ DP+ + V+ E DH D SD
Sbjct: 320 ESESESSSDDDVNNFNTKSDKEFFDALIKVKKKDPVLKQKDVKLFESDHSSEDESDDEKS 499
Query: 84 KIK 86
K K
Sbjct: 500 KDK 508
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.315 0.132 0.385
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,693,808
Number of Sequences: 36976
Number of extensions: 100698
Number of successful extensions: 577
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 567
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 571
length of query: 264
length of database: 9,014,727
effective HSP length: 94
effective length of query: 170
effective length of database: 5,538,983
effective search space: 941627110
effective search space used: 941627110
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0371b.6