BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0368c.9
(764 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG587145 similar to PIR|H86337|H8 protein F5M15.26 [imported] - ... 98 1e-20
TC83528 87 3e-17
BG644693 weakly similar to GP|18767374|g Putative 22 kDa kafirin... 81 2e-15
TC86737 weakly similar to GP|6683624|dbj|BAA89272.1 Pol {Alterna... 77 2e-14
AL367832 weakly similar to GP|14091845|gb Putative retroelement ... 75 7e-14
BG644740 similar to PIR|A84460|A84 probable retroelement pol pol... 43 4e-04
TC90865 homologue to GP|9049326|dbj|BAA99336.1 orf129b {Beta vul... 37 0.028
BG455032 weakly similar to GP|10440622|gb| putative NBS-LRR type... 32 0.91
TC90301 similar to GP|14194175|gb|AAK56282.1 AT5g58470/mqj2_60 {... 31 1.6
TC78235 weakly similar to PIR|B84420|B84420 transfactor-like pro... 31 1.6
TC79349 similar to PIR|S65668|S65668 preprotein translocase secA... 31 2.0
>BG587145 similar to PIR|H86337|H8 protein F5M15.26 [imported] - Arabidopsis
thaliana, partial (13%)
Length = 763
Score = 98.2 bits (243), Expect = 1e-20
Identities = 46/86 (53%), Positives = 62/86 (71%)
Frame = +2
Query: 421 MHSSDKDKTAFMTARANYSYQTMPFGLKNTGATYQRLMDRVFADQVGRNMEVYVGDMIVK 480
MH D +KTAF+T R Y Y+ MPFGLKN G+TYQRL++R+FAD++G MEVY+ DM+VK
Sbjct: 2 MHPDDLEKTAFITDRGTYCYKVMPFGLKNAGSTYQRLVNRMFADKLGNTMEVYIDDMLVK 181
Query: 481 SVLEANHHEELLEAFGRLRKHNMRLN 506
S+ +H L E F L ++ M+LN
Sbjct: 182 SLRATDHLNHLKE*FKTLDEYIMKLN 259
>TC83528
Length = 555
Score = 86.7 bits (213), Expect = 3e-17
Identities = 52/125 (41%), Positives = 76/125 (60%), Gaps = 4/125 (3%)
Frame = +3
Query: 77 PIVVLLRINQ-FNVQRVLLDQGSSADIIYGDAFDQLGLDERDLTPYTGSLVGFAGEQVWV 135
P+V+ L+IN F+V RV ++ S DI+Y AF ++ L E L P G L G G+ + V
Sbjct: 180 PMVIKLQINNNFSVLRVFVNPMSKVDILYWSAFLKMKLQESMLKPCQGFLKGTFGKGLPV 359
Query: 136 RGILDLDTTFCERENIKMLKVRYLVLRAMGS---YNIIIGRNTLNRLCAVISTAHTAVKY 192
+G +DLDTTF + EN K +KVRY V+ + S YN+++G +L L AV+S A +KY
Sbjct: 360 KGYIDLDTTFGKGENTKTIKVRYFVVESPPSVSIYNVVLGWPSLKDLKAVLSVAEFTIKY 539
Query: 193 LLASG 197
+ G
Sbjct: 540 PVGDG 554
>BG644693 weakly similar to GP|18767374|g Putative 22 kDa kafirin cluster;
Ty3-Gypsy type {Oryza sativa}, partial (15%)
Length = 716
Score = 80.9 bits (198), Expect = 2e-15
Identities = 54/165 (32%), Positives = 79/165 (47%)
Frame = +2
Query: 327 RMGDEKEKVIQQEVNKLLAAEFI*EIKYPTWLANVVMVKKANGK*PMCVDYTDLNKACPK 386
R+ K KV++ ++ LL FI YP + V+ +KK +G M +DY LN K
Sbjct: 92 RINPLKLKVLKLQLKDLLEKGFIQPSIYP*GVV-VLFLKKKDGFLRMSIDYPQLNNVNIK 268
Query: 387 DSYPLPSIDKLVDGASNNELLSLMDAYSGYHQIKMHSSDKDKTAFMTARANYSYQTMPFG 446
YPLP ID+L D ++ +D G HQ ++ D KTAF +Y M FG
Sbjct: 269 IKYPLPLIDELFDNLQGSKWFFKIDLRLG*HQHRVIGEDVPKTAFRIRYGHYEILVMSFG 448
Query: 447 LKNTGATYQRLMDRVFADQVGRNMEVYVGDMIVKSVLEANHHEEL 491
N + LM+RVF D + + V+ D+++ S E H L
Sbjct: 449 *TNPPMAFMELMNRVFQDYLDSLVIVFSNDILIYSKNENEHENHL 583
>TC86737 weakly similar to GP|6683624|dbj|BAA89272.1 Pol {Alternaria
alternata}, partial (21%)
Length = 1540
Score = 77.4 bits (189), Expect = 2e-14
Identities = 38/131 (29%), Positives = 67/131 (51%)
Frame = +1
Query: 359 ANVVMVKKANGK*PMCVDYTDLNKACPKDSYPLPSIDKLVDGASNNELLSLMDAYSGYHQ 418
A V+ V+K G CVDY LN KD YPLP I + + + + +D + +H+
Sbjct: 577 APVLFVRKPGGGIRFCVDYRALNAITKKDRYPLPLISETLRRVAGARWFTKLDVVAAFHK 756
Query: 419 IKMHSSDKDKTAFMTARANYSYQTMPFGLKNTGATYQRLMDRVFADQVGRNMEVYVGDMI 478
+++ D++KTAF T + + PFGL AT+QR +++ + + + Y+ D++
Sbjct: 757 MRIKDEDQEKTAFRTRYGLFEWIVCPFGLTGAPATFQRYINKTLHEFLDDFVTAYIDDVL 936
Query: 479 VKSVLEANHHE 489
+ + HE
Sbjct: 937 IYTTGSKKDHE 969
>AL367832 weakly similar to GP|14091845|gb Putative retroelement {Oryza
sativa}, partial (2%)
Length = 384
Score = 75.5 bits (184), Expect = 7e-14
Identities = 38/102 (37%), Positives = 60/102 (58%)
Frame = -1
Query: 268 LKIGTRLTESQEQRLSKLLEENLDLFAWSHKDMSGIDPNFICHRLALDPGIKLVTQTRRR 327
+K+G L E ++++ +LL E LD+FA S++DM G+DP + HR+ P V RR
Sbjct: 306 IKVGAALEEGVKRKIFQLLREYLDIFACSYEDMPGLDPKIVEHRIPTKPECPPVR*KLRR 127
Query: 328 MGDEKEKVIQQEVNKLLAAEFI*EIKYPTWLANVVMVKKANG 369
+ I+ EV K + A F+ ++YP W+AN+V V K +G
Sbjct: 126 THPDMALKIKSEVQKQIDAGFLMTVEYPEWVANIVPVPKKDG 1
>BG644740 similar to PIR|A84460|A84 probable retroelement pol polyprotein
[imported] - Arabidopsis thaliana, partial (4%)
Length = 754
Score = 43.1 bits (100), Expect = 4e-04
Identities = 19/41 (46%), Positives = 25/41 (60%)
Frame = -1
Query: 359 ANVVMVKKANGK*PMCVDYTDLNKACPKDSYPLPSIDKLVD 399
A ++ V+K +G MC+DY NK K+ YPLP ID L D
Sbjct: 238 AALLFVRKKDGYFRMCIDYRQFNKVTTKNKYPLPRIDNLFD 116
>TC90865 homologue to GP|9049326|dbj|BAA99336.1 orf129b {Beta vulgaris},
partial (16%)
Length = 940
Score = 37.0 bits (84), Expect = 0.028
Identities = 22/55 (40%), Positives = 33/55 (60%), Gaps = 1/55 (1%)
Frame = -3
Query: 88 NVQRVLLDQGSSADIIYGDAFDQLGLDERDLTPYTG-SLVGFAGEQVWVRGILDL 141
+V++++LD GSS DI+Y + F L L E++L Y G L GF G G ++L
Sbjct: 938 DVRKIILD*GSSCDIMYSNMF*TLQLLEKNLISYVGFDLQGFNGATTRP*GYVEL 774
>BG455032 weakly similar to GP|10440622|gb| putative NBS-LRR type resistance
protein 3' partial {Oryza sativa}, partial (3%)
Length = 666
Score = 32.0 bits (71), Expect = 0.91
Identities = 21/64 (32%), Positives = 33/64 (50%), Gaps = 1/64 (1%)
Frame = +1
Query: 363 MVKKANGK*PMCVDYTDLNKACPKDSYP-LPSIDKLVDGASNNELLSLMDAYSGYHQIKM 421
++K G+ C+ D+ K CPK P LPS+ L NNELL + + G Q+ +
Sbjct: 64 LLKVERGEMFPCLSSLDIWK-CPKLGLPCLPSLKDLGVDGRNNELLRSISTFRGLTQLTL 240
Query: 422 HSSD 425
+S +
Sbjct: 241 NSGE 252
>TC90301 similar to GP|14194175|gb|AAK56282.1 AT5g58470/mqj2_60 {Arabidopsis
thaliana}, partial (46%)
Length = 1222
Score = 31.2 bits (69), Expect = 1.6
Identities = 14/32 (43%), Positives = 15/32 (46%)
Frame = +1
Query: 7 PMSARSGKNSMPKEVGAIAGGFGGGGVTSAAR 38
P S G NS P G GG+GGG S R
Sbjct: 247 PTSYTGGPNSYPPSYGGNTGGYGGGDARSGGR 342
>TC78235 weakly similar to PIR|B84420|B84420 transfactor-like protein
[imported] - Arabidopsis thaliana, partial (34%)
Length = 1010
Score = 31.2 bits (69), Expect = 1.6
Identities = 27/89 (30%), Positives = 42/89 (46%), Gaps = 2/89 (2%)
Frame = +3
Query: 247 GRVNRPTPIEETKELK-FGDKVLKIGTRLTESQEQRLSKLLEENLDLFAWSHKDMSGIDP 305
G +R TP K +K G LKI + Q+ R+SKL+ E+ + +S I P
Sbjct: 141 GGADRATPKGILKGMKAMGVSELKILHVKSHLQKYRISKLIPESTTRGKIEKRSISDILP 320
Query: 306 NFI-CHRLALDPGIKLVTQTRRRMGDEKE 333
NF L L +++ + + RM D+ E
Sbjct: 321 NFCSISALQLKEVLQMQAEVQNRMSDKVE 407
>TC79349 similar to PIR|S65668|S65668 preprotein translocase secA precursor
- garden pea, partial (19%)
Length = 857
Score = 30.8 bits (68), Expect = 2.0
Identities = 16/44 (36%), Positives = 21/44 (47%)
Frame = +3
Query: 5 RRPMSARSGKNSMPKEVGAIAGGFGGGGVTSAARKRYVRAVNAV 48
RR S RSG ++ G + G F G A RK+Y VN +
Sbjct: 381 RRSRSRRSGSVAVASLGGLLGGIFKGNDTGEATRKQYAATVNVI 512
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.337 0.145 0.453
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,563,946
Number of Sequences: 36976
Number of extensions: 255631
Number of successful extensions: 1800
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 999
Number of HSP's successfully gapped in prelim test: 63
Number of HSP's that attempted gapping in prelim test: 776
Number of HSP's gapped (non-prelim): 1101
length of query: 764
length of database: 9,014,727
effective HSP length: 104
effective length of query: 660
effective length of database: 5,169,223
effective search space: 3411687180
effective search space used: 3411687180
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.7 bits)
S2: 62 (28.5 bits)
Lotus: description of TM0368c.9