BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0360.4
(968 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF641844 weakly similar to GP|15912253|gb| AT4g27870/T27E11_110 ... 244 2e-64
TC91147 210 2e-54
BF520777 similar to GP|17063191|gb| AT4g27860/T27E11_100 {Arabid... 115 1e-31
TC92210 weakly similar to GP|15912253|gb|AAL08260.1 AT4g27870/T2... 119 4e-27
TC83217 similar to GP|7303110|gb|AAF58176.1| CG12424-PA {Drosoph... 45 2e-04
BI310146 44 4e-04
TC81190 type IIB calcium ATPase [Medicago truncatula] 37 0.048
TC88464 similar to SP|Q9LU41|ACA9_ARATH Potential calcium-transp... 36 0.082
BI312045 similar to GP|22324956|gb| putative disease resistant p... 33 0.53
TC86990 similar to GP|22136840|gb|AAM91764.1 unknown protein {Ar... 32 1.2
TC77624 similar to PIR|T12196|T12196 RNA-binding protein - fava ... 30 3.4
TC89456 weakly similar to PIR|B84583|B84583 hypothetical protein... 30 3.4
TC77868 similar to GP|11994207|dbj|BAB01310. gene_id:MPN9.20~unk... 30 5.9
TC85664 1-aminocyclopropanecarboxylic acid oxidase [Medicago tru... 30 5.9
TC92271 similar to PIR|T51174|T51174 ataxia-telangiectasia mutat... 29 7.6
AW689795 weakly similar to PIR|T11678|T116 hypothetical protein ... 29 7.6
TC77206 similar to GP|18377735|gb|AAL67017.1 putative alpha-gala... 29 7.6
>BF641844 weakly similar to GP|15912253|gb| AT4g27870/T27E11_110 {Arabidopsis
thaliana}, partial (16%)
Length = 644
Score = 244 bits (622), Expect = 2e-64
Identities = 130/201 (64%), Positives = 156/201 (76%)
Frame = +1
Query: 664 VVASNKQTSGIDAIFPSKPDLAPIEEVQKDINKEISASDKKESKGGDVIVVVDREAIEST 723
V + + +S ID IFPSK D+ I+ V+KDIN +I+ S E+KG DVIVVVD EA EST
Sbjct: 13 VQSFDSTSSAIDGIFPSKTDITLIDTVRKDINGKINPSVINENKG-DVIVVVDEEANEST 189
Query: 724 TSQTADNVPVEGAVVPGSDTQVLIDEQPRDETGERQQWEVLKSIVYGGLVESITSLGIVS 783
T QT NVP +GA+V S TQV I EQPR+E GE ++WE++KSIVYGGLVESITSLGIVS
Sbjct: 190 TLQTEGNVPRDGAIVTESPTQVDIGEQPRNEVGEPKKWEIVKSIVYGGLVESITSLGIVS 369
Query: 784 SAVSSGAAPLNIIALGLANLVGGLFILGHNLNDLKNNHSGGDQQQTNAQEDQYHELLGRR 843
SA SSGA PLNII LG ANL+GGLFILGHNL +LK++HS G Q QTN Q D+Y ELLG R
Sbjct: 370 SAASSGATPLNIITLGFANLIGGLFILGHNLKELKDSHSRGQQLQTNVQ-DRYQELLGNR 546
Query: 844 ANFLLHAVVAVISFIIFGAVP 864
+NF+ HAV+A SF+IFG+ P
Sbjct: 547 SNFVFHAVIAXFSFLIFGSAP 609
>TC91147
Length = 713
Score = 210 bits (534), Expect = 2e-54
Identities = 134/294 (45%), Positives = 169/294 (56%), Gaps = 5/294 (1%)
Frame = +1
Query: 341 RCLACFSFFIPSGKGFNLFRNFGGSRKQETSQNATSITAQNLQNPSTLPGSNANWFTSLF 400
RCL+CFS FIPSGKGFNLFRNFGG+ K ETSQN+ S +P S+ NWF SLF
Sbjct: 1 RCLSCFSIFIPSGKGFNLFRNFGGASKDETSQNS-----------SNIPSSSPNWFISLF 147
Query: 401 TSNKGKTATAKGNTSLEHSTTAHVEQHCSTSVTSNVPASTDIGHLEGSLADTALIKNAKP 460
TSNK KTAT G+TS E+STT +Q+ ST +TS + +S+DIGH EG+L D LIKN KP
Sbjct: 148 TSNKRKTATEPGDTSQEYSTTDPADQNQST-ITSPLLSSSDIGHPEGTLGDADLIKNVKP 324
Query: 461 AAENGGMSSQISSTAESVKIGPGTENGIKFHTASGNEPLADQKCLDSSFQNDFSSIKSTG 520
++ +++ST S
Sbjct: 325 TSDVNHGRERMNSTISS------------------------------------------- 375
Query: 521 GVMNGVQSAGQDFIDLSSKEQLLTGNLKTND---GETSVDIIKTTANVEVMSSMSFSDGV 577
NGVQS QDFID S KEQ LT L+T++ +TSVD T VEV SS +FS+G
Sbjct: 376 ---NGVQSVVQDFIDFSEKEQSLTRKLRTDNRGKNKTSVDTTNTN-TVEVTSSTNFSNGT 543
Query: 578 VSKYESVQSVTTATSEKHFNAGETAKVSVLNPSEGIPGFLAP--TTIGSLILEQ 629
VS+Y+SV+SVTT +SE N+G TAK ++LN + P F+ P TTI SLI E+
Sbjct: 544 VSEYKSVKSVTTTSSETFVNSGATAKGAILNHYQEKPEFIVPTSTTIASLIXEK 705
>BF520777 similar to GP|17063191|gb| AT4g27860/T27E11_100 {Arabidopsis
thaliana}, partial (5%)
Length = 627
Score = 115 bits (287), Expect(2) = 1e-31
Identities = 61/84 (72%), Positives = 68/84 (80%)
Frame = +2
Query: 709 GDVIVVVDREAIESTTSQTADNVPVEGAVVPGSDTQVLIDEQPRDETGERQQWEVLKSIV 768
GDVIVVVD EA ESTT QT NVP +GA+V S TQV I EQP +E GE ++WE++KSIV
Sbjct: 2 GDVIVVVDEEANESTTLQTEGNVPRDGAIVTESPTQVDIGEQPINEVGEPKKWEIVKSIV 181
Query: 769 YGGLVESITSLGIVSSAVSSGAAP 792
YGGLVESITSLGIVSSA SSGA P
Sbjct: 182 YGGLVESITSLGIVSSAASSGATP 253
Score = 40.8 bits (94), Expect(2) = 1e-31
Identities = 17/23 (73%), Positives = 21/23 (90%)
Frame = +3
Query: 793 LNIIALGLANLVGGLFILGHNLN 815
+NII LG ANL+GGLFILGHN++
Sbjct: 369 VNIITLGFANLIGGLFILGHNVS 437
>TC92210 weakly similar to GP|15912253|gb|AAL08260.1 AT4g27870/T27E11_110
{Arabidopsis thaliana}, partial (17%)
Length = 536
Score = 119 bits (299), Expect = 4e-27
Identities = 58/128 (45%), Positives = 86/128 (66%), Gaps = 2/128 (1%)
Frame = +1
Query: 826 QQQTNAQEDQYHELLGRRANFLLHAVVAVISFIIFGAVPLAIYGVLINKNYSSDVKLGIV 885
+ T A DQY+E+LG+R NF+LHA +A++SFIIFG +P +YG +N D KL V
Sbjct: 4 RSNTEAVVDQYNEVLGQRKNFILHAFIAILSFIIFGLIPPVVYGFTFRENDEKDFKLAAV 183
Query: 886 AATSVVCIILLAIAKVYTRKPPKY--YFKTMLYYVALALGASGISYIAGILIKELLEKIS 943
A S++CI LL+IAK Y ++P Y YF+T+ YYV+ A+ +SY+AG ++K+L+EK+
Sbjct: 184 AGASLLCITLLSIAKAYIKRPNSYSTYFQTVFYYVSTGAVATVLSYLAGDMMKKLIEKLG 363
Query: 944 NSESGFAF 951
E +F
Sbjct: 364 WFEPASSF 387
>TC83217 similar to GP|7303110|gb|AAF58176.1| CG12424-PA {Drosophila
melanogaster}, partial (1%)
Length = 629
Score = 44.7 bits (104), Expect = 2e-04
Identities = 30/105 (28%), Positives = 51/105 (48%), Gaps = 2/105 (1%)
Frame = +1
Query: 169 IENSVTQRNLCSEVNDQCMKTVDDK--VPSNSEVSPLHNSRDDTRATTVLDFHEETNIKE 226
IE+ T+ E+ ++ + + K + N + P + +T++++ TN
Sbjct: 313 IESDETKPKFEVEIKERNAEMIIQKEDIDENDTILPASDPPLPEDSTSLINV---TNESG 483
Query: 227 GSELVKEIDQDLKEFDVEVVLAKQETHDLFCPNCNSCITKRVILK 271
+V+E Q+ +EF +E V K TH +CPNCN CI K I K
Sbjct: 484 TVIVVEENGQERQEFYLEKVFEKPPTHGFYCPNCNVCIQKVYIQK 618
>BI310146
Length = 796
Score = 43.5 bits (101), Expect = 4e-04
Identities = 23/55 (41%), Positives = 34/55 (61%), Gaps = 2/55 (3%)
Frame = +3
Query: 752 RDETGERQQW--EVLKSIVYGGLVESITSLGIVSSAVSSGAAPLNIIALGLANLV 804
R+ ++ W E +KSIVYGGL IT ++SS +S ++ N++ LG ANLV
Sbjct: 150 RNSERPKEPWRGEYVKSIVYGGLDAIITCFSLISSISASSSSSGNVLVLGFANLV 314
>TC81190 type IIB calcium ATPase [Medicago truncatula]
Length = 3277
Score = 36.6 bits (83), Expect = 0.048
Identities = 58/257 (22%), Positives = 106/257 (40%), Gaps = 17/257 (6%)
Frame = +1
Query: 707 KGGDVIVVVDREAIESTTSQTAD---NVPVEGAVVPGSDTQVLIDEQPRDETGERQQW-- 761
K G V+ V ++ + AD ++ ++G V + ++I + + +W
Sbjct: 2320 KKGHVVAVTGDGTNDAPALKEADIGLSMGIQGTEVAKESSDIVILDDNFNSVATVLRWGR 2499
Query: 762 ---EVLKSIVYGGLVESITSLGI-VSSAVSSGAAPLNIIALGLANLV----GGLFILGHN 813
++ + L ++ +L I +AVSSG PL + L NL+ G L +
Sbjct: 2500 CVYNNIQKFIQFQLTVNVAALVINFIAAVSSGDVPLTTVQLLWVNLIMDTLGALAL---- 2667
Query: 814 LNDLKNNHSGGDQQQTNAQEDQYHELLGRRA----NFLLHAVVAVISFIIFGAVPLAIYG 869
+ +E + +GR A N + ++A S+ I + + YG
Sbjct: 2668 ------------ATERPTKELMKKKPIGRTAPLITNIMWRNLLAQASYQIAVLLIMQFYG 2811
Query: 870 VLINKNYSSDVKLGIVAATSVVCIILLAIAKVYTRKPPKYYFKTMLYYVALALGASGISY 929
I N S +VK ++ T V+C + + +R K Y + L LG GI+
Sbjct: 2812 KSI-FNVSKEVKDTLIFNTFVLCQV---FNEFNSRSMEKLYVFEGILKNHLFLGIIGITI 2979
Query: 930 IAGILIKELLEKISNSE 946
+ IL+ ELL K +++E
Sbjct: 2980 VLQILMVELLRKFADTE 3030
>TC88464 similar to SP|Q9LU41|ACA9_ARATH Potential calcium-transporting
ATPase 9 plasma membrane-type (EC 3.6.3.8) (Ca2+-ATPase
isoform 9)., partial (43%)
Length = 1608
Score = 35.8 bits (81), Expect = 0.082
Identities = 55/252 (21%), Positives = 102/252 (39%), Gaps = 13/252 (5%)
Frame = +3
Query: 707 KGGDVIVVVDREAIESTTSQTAD---NVPVEGAVVPGSDTQVLIDEQPRDETGERQQW-- 761
KGGDV+ V ++ AD ++ ++G V + ++I + + +W
Sbjct: 561 KGGDVVAVTGDGTNDAPALHEADIGLSMGIQGTEVAKESSDIIILDDNFASVVKVVRWGR 740
Query: 762 ---EVLKSIVYGGLVESITSLGI-VSSAVSSGAAPLNIIALGLANLV----GGLFILGHN 813
++ + L ++ +L I V +A+SSG PLN + L NL+ G L +
Sbjct: 741 SVYANIQKFIQFQLTVNVAALVINVVAAISSGDVPLNAVQLLWVNLIMDTLGALALATEP 920
Query: 814 LNDLKNNHSGGDQQQTNAQEDQYHELLGRRANFLLHAVVAVISFIIFGAVPLAIYGVLIN 873
D + S +E ++ R N ++ A+ V + +I
Sbjct: 921 PTDHLMHRS-----PVGRREPLITNIMWR--NLIVQALYQVSVLLFLNFCGESILPKQDT 1079
Query: 874 KNYSSDVKLGIVAATSVVCIILLAIAKVYTRKPPKYYFKTMLYYVALALGASGISYIAGI 933
K + VK ++ V+C I + RKP + + L +G GI++I I
Sbjct: 1080KAHDYQVKNTLIFNAFVMCQI---FNEFNARKPDEMNVFPGVTKNRLFMGIIGITFILQI 1250
Query: 934 LIKELLEKISNS 945
+I E L K +++
Sbjct: 1251VIIEFLGKFAST 1286
>BI312045 similar to GP|22324956|gb| putative disease resistant protein
{Oryza sativa (japonica cultivar-group)}, partial (1%)
Length = 800
Score = 33.1 bits (74), Expect = 0.53
Identities = 14/30 (46%), Positives = 19/30 (62%), Gaps = 2/30 (6%)
Frame = -1
Query: 327 YHPEREPES--DPEVFRCLACFSFFIPSGK 354
+ E+ P S P+V++CL C SF IP GK
Sbjct: 185 FDSEQSPRSLIGPQVYKCLICLSFVIPQGK 96
>TC86990 similar to GP|22136840|gb|AAM91764.1 unknown protein {Arabidopsis
thaliana}, partial (64%)
Length = 2354
Score = 32.0 bits (71), Expect = 1.2
Identities = 22/89 (24%), Positives = 40/89 (44%), Gaps = 7/89 (7%)
Frame = +2
Query: 664 VVASNKQTSGIDAIFPSKPDLAPIEEVQKDINKEISASDKKESKGGDVIV-------VVD 716
VV S+K I + A ++E+QKD++ + + KE++ G I +
Sbjct: 1076 VVKSSKAARLIMVFLDTSHTNASMDEIQKDLSPLVKQLEPKENENGAQIPFLMASDGIKK 1255
Query: 717 REAIESTTSQTADNVPVEGAVVPGSDTQV 745
R ++ TS ++ VE V D++V
Sbjct: 1256 RNIVDQITSSLTGSIIVEDVVYENVDSEV 1342
>TC77624 similar to PIR|T12196|T12196 RNA-binding protein - fava bean
(fragment), partial (90%)
Length = 1214
Score = 30.4 bits (67), Expect = 3.4
Identities = 23/58 (39%), Positives = 32/58 (54%), Gaps = 1/58 (1%)
Frame = +1
Query: 898 IAKVYTRKPPKYYFKTMLYYVALALGASGIS-YIAGILIKELLEKISNSESGFAFITM 954
+A+V TR K YF + Y V AL A I Y + LI+ L ++ + GFAF+TM
Sbjct: 370 VAEVDTRT--KLYFGNLPYSVDSALLAGLIEEYGSAELIEVLYDRDTGKSRGFAFVTM 537
>TC89456 weakly similar to PIR|B84583|B84583 hypothetical protein At2g19950
[imported] - Arabidopsis thaliana, partial (7%)
Length = 1100
Score = 30.4 bits (67), Expect = 3.4
Identities = 38/183 (20%), Positives = 72/183 (38%), Gaps = 10/183 (5%)
Frame = +3
Query: 582 ESVQSVTTATSEKHFNAGETAKVSVLNPSEGIPGFLAPTTIGSLILEQS--QKDVDGTSE 639
+S ++ T EK+ + T+ VSV PS+ + S+ QS Q+ D S
Sbjct: 522 DSSTIISDTTKEKNESPAATSDVSVTTPSDKVDPVAKNDGSDSISTNQSKEQQPTDAVSP 701
Query: 640 NIKNSYSSFTQSSVQSSGSSVPASVVASNKQTSGIDAIFPSKPDLAPIEEVQKDIN---- 695
+ S S + + S V + + +D + + P+ P++E DIN
Sbjct: 702 VLGTSLS----TKLASDTVKHDTGDVEVLRNDTDVD-VTTTSPNNEPVKEKASDINEVDP 866
Query: 696 ----KEISASDKKESKGGDVIVVVDREAIESTTSQTADNVPVEGAVVPGSDTQVLIDEQP 751
K I + + + + VD E+ ++ + + V + V P + +L D
Sbjct: 867 SPSPKGIKDPNHEPTSTDQITKAVDLESNQNMDQEETETVADD--VAPSTSDTILKDSDI 1040
Query: 752 RDE 754
+ E
Sbjct: 1041KAE 1049
>TC77868 similar to GP|11994207|dbj|BAB01310. gene_id:MPN9.20~unknown
protein {Arabidopsis thaliana}, partial (36%)
Length = 1019
Score = 29.6 bits (65), Expect = 5.9
Identities = 17/60 (28%), Positives = 27/60 (44%), Gaps = 5/60 (8%)
Frame = +1
Query: 593 EKHFNA----GETAKVSVLNPSEGIPGFLAPTTIGSLILEQS-QKDVDGTSENIKNSYSS 647
+ H N G + + NPSE PGF P+ +G L ++ + +EN N Y +
Sbjct: 400 QNHLNGLHADGANIQFEINNPSESEPGFRVPSNLGDYFLGAGFEQLIQQLAENDPNRYGT 579
>TC85664 1-aminocyclopropanecarboxylic acid oxidase [Medicago truncatula]
Length = 1832
Score = 29.6 bits (65), Expect = 5.9
Identities = 17/51 (33%), Positives = 26/51 (50%)
Frame = +1
Query: 209 DTRATTVLDFHEETNIKEGSELVKEIDQDLKEFDVEVVLAKQETHDLFCPN 259
D +T L E+NI E +L E + +KEF +++ +E DL C N
Sbjct: 889 DWESTFHLRHLPESNISEIPDLSDEYRKSMKEFALKLETLAEELLDLLCEN 1041
>TC92271 similar to PIR|T51174|T51174 ataxia-telangiectasia mutated protein
(Atm) [imported] - Arabidopsis thaliana, partial (1%)
Length = 830
Score = 29.3 bits (64), Expect = 7.6
Identities = 34/117 (29%), Positives = 53/117 (45%), Gaps = 6/117 (5%)
Frame = -1
Query: 651 SSVQSSGSSVPASVVASNKQ-TSGIDAIF-----PSKPDLAPIEEVQKDINKEISASDKK 704
S+VQ+ V + +S+K T+G + + PSK LAP K + E S S +
Sbjct: 323 SNVQNLSVKVHLTSCSSHKTPTTGSELYYMIMKGPSKNHLAPSRCALKPL*LEQSISISR 144
Query: 705 ESKGGDVIVVVDREAIESTTSQTADNVPVEGAVVPGSDTQVLIDEQPRDETGERQQW 761
+ K G I +V RE NV + +VV + LIDE+ E+ + +W
Sbjct: 143 KDKFGYEIFLVVREV----------NVHLFLSVVVAAALWFLIDERDCIESSKMLRW 3
>AW689795 weakly similar to PIR|T11678|T116 hypothetical protein
SPBC21D10.06c - fission yeast (Schizosaccharomyces
pombe), partial (3%)
Length = 678
Score = 29.3 bits (64), Expect = 7.6
Identities = 31/126 (24%), Positives = 53/126 (41%), Gaps = 5/126 (3%)
Frame = +3
Query: 376 SITAQNLQNPSTLPGSNANWFTSLFTSNKGKTATAKGNTSLEHSTTAHVEQHCSTSVTSN 435
+ T+QN+ + STL S+++ S T+T N S S++A V S + +
Sbjct: 3 NFTSQNMFSSSTLATSSSSISAPATFSTSMTTSTIAVNVSSSSSSSASVTTSSSPTTSLF 182
Query: 436 VPASTDIGHLEGSLADTALIKNAKPAAENGGMSSQI-----SSTAESVKIGPGTENGIKF 490
ST L S++ ++P GG ++ I SS+A S + +
Sbjct: 183 KAGSTP---LPSSVSLPVSSSGSEPVETKGGQNAGIGIFGFSSSATSANSQSQIQGSVFG 353
Query: 491 HTASGN 496
T SG+
Sbjct: 354 STISGS 371
>TC77206 similar to GP|18377735|gb|AAL67017.1 putative alpha-galactosidase
{Arabidopsis thaliana}, partial (80%)
Length = 1786
Score = 29.3 bits (64), Expect = 7.6
Identities = 10/22 (45%), Positives = 15/22 (67%)
Frame = -2
Query: 257 CPNCNSCITKRVILKKRKRNIH 278
CP+CN+C T + KR+ N+H
Sbjct: 723 CPDCNNCHTSNNLRPKRQINLH 658
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.309 0.127 0.347
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,580,410
Number of Sequences: 36976
Number of extensions: 276907
Number of successful extensions: 1365
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 1339
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1358
length of query: 968
length of database: 9,014,727
effective HSP length: 105
effective length of query: 863
effective length of database: 5,132,247
effective search space: 4429129161
effective search space used: 4429129161
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 63 (28.9 bits)
Lotus: description of TM0360.4