BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0348.6
(408 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC77850 weakly similar to PIR|H96517|H96517 protein T2E6.22 [imp... 395 e-110
BG646718 weakly similar to PIR|H96517|H96 protein T2E6.22 [impor... 100 2e-27
BF637378 weakly similar to PIR|H96517|H965 protein T2E6.22 [impo... 115 2e-26
BF006197 weakly similar to GP|1310677|emb| protein z-type serpin... 67 1e-11
AW560154 50 2e-06
TC91113 similar to GP|20196895|gb|AAM14825.1 putative guanylate ... 32 0.58
TC83265 similar to GP|10047457|gb|AAG12252.1 guanylate kinase {L... 32 0.58
TC83145 weakly similar to GP|16604509|gb|AAL24260.1 AT5g58730/mz... 30 1.3
BM814136 similar to PIR|G96841|G9684 hypothetical protein F23A5.... 28 4.9
TC89137 similar to GP|4200338|emb|CAA76726.1 P69C protein {Lycop... 28 6.4
TC87659 similar to GP|10177021|dbj|BAB10259. contains similarity... 28 6.4
BI266140 similar to GP|20521428|db putative tryptophanyl-tRNA sy... 28 8.4
>TC77850 weakly similar to PIR|H96517|H96517 protein T2E6.22 [imported] -
Arabidopsis thaliana, partial (65%)
Length = 1485
Score = 395 bits (1015), Expect = e-110
Identities = 213/390 (54%), Positives = 285/390 (72%), Gaps = 1/390 (0%)
Frame = +2
Query: 12 QSDVALSFTKHLFSKEDYQEKNLIFSPLSLYAALSVMAAGSEGRTLDELLSFLRFDSIDH 71
Q++V+LS KHLFSKE + N++FSPLSL LS++A+GSEG T +L +FL+ S DH
Sbjct: 68 QTNVSLSVAKHLFSKES--DNNIVFSPLSLQVVLSIIASGSEGPTQQQLFNFLQSKSTDH 241
Query: 72 LNTYFSQGISPVFSDEDPASSPSQHHLSFTNGMFIDTTVSLSYPFRRLLSTHYNASLASL 131
LN + SQ +S + SD PA P LSF +G+++D T+SL F++++STH+ A+L+S+
Sbjct: 242 LNYFASQLVSVILSDASPAGGPL---LSFVDGVWVDQTLSLQPSFQQIVSTHFKAALSSV 412
Query: 132 DFNLRGDSVLHDVNSLIEQESNGLITQLLPPGTVTNLTKLIFANALRFQGMWKHTLDGL- 190
DF + V ++VNS E+E+NGLI +LLP G+V N T+LIFANAL F+G W D
Sbjct: 413 DFQNKAVEVTNEVNSWAEKETNGLIKELLPLGSVNNATRLIFANALYFKGAWNDKFDASK 592
Query: 191 THVSSFNLLSGTSVKVPYMTTFKKTQYVRAFDGFKILRLPYKQGRDRQRRFSMCIFLPDA 250
T F+LL+G+ VKVP+MT+ KK Q++RAFDGFK+L LPYKQG D+ R+F+M FLP+A
Sbjct: 593 TEDYEFHLLNGSPVKVPFMTS-KKKQFIRAFDGFKVLGLPYKQGEDK-RQFTMYFFLPNA 766
Query: 251 QDGLSALIQKLSSEPGFLKGKLPHRKVRVRPFRVPKFNISFTFEASNVLKEVGVVSPFSP 310
+DGL+AL++K++SE L+ KLP KV V FR+PKFNISF E S++LKE+GVV PFS
Sbjct: 767 KDGLAALVEKVASESELLQHKLPFGKVEVGDFRIPKFNISFGLETSDMLKELGVVLPFS- 943
Query: 311 MDAHFTKMVNVNSPSDNLFVQSIFHKAFIEVNEKGTKATAATWSALARQCARDHHPSIDF 370
TKMVN +S S NL V +IFHK+FIEVNE+GT+A AAT + + + A P +DF
Sbjct: 944 -GGGLTKMVN-SSVSQNLCVSNIFHKSFIEVNEEGTEAAAATAATILLRSAMSIPPRLDF 1117
Query: 371 IADHPFLFLIREDFTGTILFVGQVLNPLDG 400
+ADHPFLF+IRED TGTI+FVGQVLNPL G
Sbjct: 1118VADHPFLFMIREDLTGTIIFVGQVLNPLAG 1207
>BG646718 weakly similar to PIR|H96517|H96 protein T2E6.22 [imported] -
Arabidopsis thaliana, partial (30%)
Length = 702
Score = 100 bits (250), Expect(2) = 2e-27
Identities = 51/108 (47%), Positives = 73/108 (67%)
Frame = +2
Query: 81 SPVFSDEDPASSPSQHHLSFTNGMFIDTTVSLSYPFRRLLSTHYNASLASLDFNLRGDSV 140
S V SD A P HLSF +G++I+ T+SL F++++S+ Y A+LAS+DF + V
Sbjct: 224 SIVLSDAASAGGP---HLSFVDGVWIEKTLSLQPSFKQIMSSDYKATLASVDFMFKAPEV 394
Query: 141 LHDVNSLIEQESNGLITQLLPPGTVTNLTKLIFANALRFQGMWKHTLD 188
+VN +E+E+NGLI +L+PPG+V +LT LIFANAL F+G W D
Sbjct: 395 RKEVNIWVEKETNGLIKELIPPGSVDSLTSLIFANALYFRGAWNEKFD 538
Score = 39.3 bits (90), Expect(2) = 2e-27
Identities = 19/37 (51%), Positives = 26/37 (69%)
Frame = +1
Query: 12 QSDVALSFTKHLFSKEDYQEKNLIFSPLSLYAALSVM 48
Q+ V+L KHLFSK+ E N++FSPLSL LS++
Sbjct: 34 QTRVSLRIAKHLFSKQS--ENNIVFSPLSLQVVLSIL 138
Score = 28.9 bits (63), Expect = 3.8
Identities = 19/42 (45%), Positives = 24/42 (56%), Gaps = 1/42 (2%)
Frame = +3
Query: 41 LYAALSVMAAG-SEGRTLDELLSFLRFDSIDHLNTYFSQGIS 81
L+A +S A S+ T +LL FLR S DHLN SQ +S
Sbjct: 99 LFAIVSASCAQHSDCPTPQQLLDFLRSKSTDHLNYLASQLVS 224
>BF637378 weakly similar to PIR|H96517|H965 protein T2E6.22 [imported] -
Arabidopsis thaliana, partial (9%)
Length = 627
Score = 115 bits (289), Expect = 2e-26
Identities = 60/133 (45%), Positives = 88/133 (66%)
Frame = +1
Query: 1 MEIQKSKSTRCQSDVALSFTKHLFSKEDYQEKNLIFSPLSLYAALSVMAAGSEGRTLDEL 60
M Q+ +S+ Q+ V+L+ KHLFSKE EKN++FSPLSL LS++ AGSEG T +L
Sbjct: 1 MATQRDESSNNQTKVSLTIVKHLFSKES--EKNIVFSPLSLQVVLSIITAGSEGSTQQQL 174
Query: 61 LSFLRFDSIDHLNTYFSQGISPVFSDEDPASSPSQHHLSFTNGMFIDTTVSLSYPFRRLL 120
L FLRF S DHLN+ S +S + D + P HLSF +G++++ T+SL F++++
Sbjct: 175 LDFLRFKSTDHLNSSVSHQLSIILKDATSSGGP---HLSFVDGVWLEKTLSLQSSFKQIV 345
Query: 121 STHYNASLASLDF 133
S Y A+L+ +DF
Sbjct: 346 SEDYKATLSEVDF 384
>BF006197 weakly similar to GP|1310677|emb| protein z-type serpin {Hordeum
vulgare subsp. vulgare}, partial (7%)
Length = 468
Score = 67.0 bits (162), Expect = 1e-11
Identities = 36/87 (41%), Positives = 52/87 (59%)
Frame = +3
Query: 47 VMAAGSEGRTLDELLSFLRFDSIDHLNTYFSQGISPVFSDEDPASSPSQHHLSFTNGMFI 106
++ A EGRT +LL FLR IDHLN++ S +S + SD P+ LS T +++
Sbjct: 3 IVTAACEGRTQQQLLEFLRSKFIDHLNSFTSHLVSIILSDAAPSGG---SRLSLTQRVWV 173
Query: 107 DTTVSLSYPFRRLLSTHYNASLASLDF 133
D T+SL F+ + T Y A+LAS+DF
Sbjct: 174 DQTLSLQPSFKETMVTDYKATLASVDF 254
>AW560154
Length = 470
Score = 50.1 bits (118), Expect = 2e-06
Identities = 26/64 (40%), Positives = 39/64 (60%)
Frame = +1
Query: 70 DHLNTYFSQGISPVFSDEDPASSPSQHHLSFTNGMFIDTTVSLSYPFRRLLSTHYNASLA 129
DHLN++ S +S + SD P+ LSFT +++D T+SL F+ + T Y A+LA
Sbjct: 1 DHLNSFTSDLVSIILSDAAPSGGS---RLSFTQRVWVDQTLSLQPSFKETMVTDYKATLA 171
Query: 130 SLDF 133
S+DF
Sbjct: 172 SVDF 183
>TC91113 similar to GP|20196895|gb|AAM14825.1 putative guanylate kinase
{Arabidopsis thaliana}, partial (66%)
Length = 1367
Score = 31.6 bits (70), Expect = 0.58
Identities = 23/60 (38%), Positives = 31/60 (51%)
Frame = +1
Query: 51 GSEGRTLDELLSFLRFDSIDHLNTYFSQGISPVFSDEDPASSPSQHHLSFTNGMFIDTTV 110
GS+ + LL FLR S +++TYFS G +P F +D P + L NG TTV
Sbjct: 121 GSKEQNTSFLLRFLRV*S-RNIDTYFSMGEAPAFLVDDLQDGP-LNGLELNNGTCKTTTV 294
>TC83265 similar to GP|10047457|gb|AAG12252.1 guanylate kinase {Lilium
longiflorum}, partial (24%)
Length = 719
Score = 31.6 bits (70), Expect = 0.58
Identities = 23/60 (38%), Positives = 31/60 (51%)
Frame = +3
Query: 51 GSEGRTLDELLSFLRFDSIDHLNTYFSQGISPVFSDEDPASSPSQHHLSFTNGMFIDTTV 110
GS+ + LL FLR S +++TYFS G +P F +D P + L NG TTV
Sbjct: 138 GSKEQNTSFLLRFLRV*S-RNIDTYFSMGEAPAFLVDDLQDGP-LNGLELNNGTCKTTTV 311
>TC83145 weakly similar to GP|16604509|gb|AAL24260.1 AT5g58730/mzn1_180
{Arabidopsis thaliana}, partial (33%)
Length = 904
Score = 30.4 bits (67), Expect = 1.3
Identities = 13/46 (28%), Positives = 22/46 (47%)
Frame = -2
Query: 61 LSFLRFDSIDHLNTYFSQGISPVFSDEDPASSPSQHHLSFTNGMFI 106
++ RF I+H F + ++P+F D ++P H L FI
Sbjct: 855 INLTRFKRINHQTPIFRENLTPIFPMRDHHATPFSHFLQINKLSFI 718
>BM814136 similar to PIR|G96841|G9684 hypothetical protein F23A5.25
[imported] - Arabidopsis thaliana, partial (46%)
Length = 576
Score = 28.5 bits (62), Expect = 4.9
Identities = 14/54 (25%), Positives = 24/54 (43%)
Frame = +1
Query: 335 HKAFIEVNEKGTKATAATWSALARQCARDHHPSIDFIADHPFLFLIREDFTGTI 388
+ F+ ++ T TW L +Q A+D HP + D F+ F+ T+
Sbjct: 127 YHGFL*TEDQNTNLIKITWKKLIKQGAKDSHPVLLLPNDFEKHFIKEHSFSVTL 288
>TC89137 similar to GP|4200338|emb|CAA76726.1 P69C protein {Lycopersicon
esculentum}, partial (37%)
Length = 1230
Score = 28.1 bits (61), Expect = 6.4
Identities = 14/39 (35%), Positives = 22/39 (55%)
Frame = +3
Query: 172 IFANALRFQGMWKHTLDGLTHVSSFNLLSGTSVKVPYMT 210
I L W +LD T SSFN+++GTS+ P+++
Sbjct: 411 ILGPGLNILAGWPISLDNST--SSFNIIAGTSMSCPHLS 521
>TC87659 similar to GP|10177021|dbj|BAB10259. contains similarity to sorting
nexin~gene_id:MQJ2.4 {Arabidopsis thaliana}, partial
(70%)
Length = 1803
Score = 28.1 bits (61), Expect = 6.4
Identities = 13/23 (56%), Positives = 14/23 (60%)
Frame = +3
Query: 80 ISPVFSDEDPASSPSQHHLSFTN 102
+SPV D DP SP QHH F N
Sbjct: 375 VSPV--DSDPLLSPPQHHREFQN 437
>BI266140 similar to GP|20521428|db putative tryptophanyl-tRNA synthetase
{Oryza sativa (japonica cultivar-group)}, partial (38%)
Length = 610
Score = 27.7 bits (60), Expect = 8.4
Identities = 18/53 (33%), Positives = 27/53 (49%), Gaps = 2/53 (3%)
Frame = +1
Query: 268 LKGKLPHRKVRVRPFRVPKFN--ISFTFEASNVLKEVGVVSPFSPMDAHFTKM 318
L G L H + R P+FN + FT E ++V +G+ PFS HF+ +
Sbjct: 163 LHGLLLHPQPLSRNELSPEFNLPVQFTLETTSVPSRIGL--PFSMCTIHFSSL 315
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.322 0.136 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,016,145
Number of Sequences: 36976
Number of extensions: 161229
Number of successful extensions: 796
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 784
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 786
length of query: 408
length of database: 9,014,727
effective HSP length: 98
effective length of query: 310
effective length of database: 5,391,079
effective search space: 1671234490
effective search space used: 1671234490
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0348.6