BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0346.4
(142 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG587938 homologue to GP|22655312|g putative ABC transporter {Ar... 68 5e-26
AW775117 similar to GP|22655312|g putative ABC transporter {Arab... 92 9e-20
TC90210 similar to GP|14715462|dbj|BAB62040. CjMDR1 {Coptis japo... 79 7e-17
TC79211 similar to GP|4204793|gb|AAD10836.1| P-glycoprotein {Sol... 81 1e-16
TC78215 similar to GP|10177549|dbj|BAB10828. ABC transporter-lik... 73 4e-14
TC90288 weakly similar to GP|21645421|gb|AAF58271.2 CG8523-PA {D... 72 7e-14
TC77937 similar to GP|14715462|dbj|BAB62040. CjMDR1 {Coptis japo... 72 1e-13
TC77935 similar to PIR|E85023|E85023 probable P-glycoprotein-lik... 71 2e-13
TC79335 similar to GP|14715462|dbj|BAB62040. CjMDR1 {Coptis japo... 67 4e-13
TC81811 similar to GP|6671365|gb|AAF23176.1| P-glycoprotein {Gos... 69 5e-13
BG646029 similar to GP|14715462|d CjMDR1 {Coptis japonica}, part... 64 8e-13
TC83850 weakly similar to GP|7300071|gb|AAF55241.1| CG4225 gene ... 56 4e-09
TC80024 homologue to PIR|T47671|T47671 P-glycoprotein-like - Ara... 55 7e-09
BM780003 weakly similar to PIR|T06165|T0 multidrug resistance pr... 54 2e-08
BQ751194 similar to GP|21622336|em probable multidrug resistance... 47 3e-06
TC89340 weakly similar to GP|17427488|emb|CAD14006. PROBABLE COM... 40 4e-04
TC80066 similar to PIR|T50518|T50518 ABC transporter-like protei... 37 0.002
AJ497912 similar to GP|8777328|dbj| ABC transporter-like protein... 34 0.017
TC87489 homologue to PIR|T52080|T52080 multi resistance protein ... 32 0.11
TC87490 similar to PIR|T52080|T52080 multi resistance protein [i... 30 0.25
>BG587938 homologue to GP|22655312|g putative ABC transporter {Arabidopsis
thaliana}, partial (19%)
Length = 780
Score = 67.8 bits (164), Expect(2) = 5e-26
Identities = 30/41 (73%), Positives = 38/41 (92%)
Frame = +2
Query: 14 KSATLWKAKTENSDSKGNAEKTSNSSPR*GNKCIGL*VRKA 54
++ T W+ KT+NS+SKGNA+K+SNSSPR*GNKCIGL*+RKA
Sbjct: 551 RTTTFWRTKTKNSNSKGNAKKSSNSSPR*GNKCIGL*IRKA 673
Score = 65.5 bits (158), Expect(2) = 5e-26
Identities = 33/37 (89%), Positives = 35/37 (94%)
Frame = +3
Query: 53 KAVQEALDRFMIGRTTLVIAHRLSTIRKADLVAVLQQ 89
K VQEALDRFMIGRTTLVIAHRLSTIRKADLVAV+ +
Sbjct: 669 KLVQEALDRFMIGRTTLVIAHRLSTIRKADLVAVIHK 779
>AW775117 similar to GP|22655312|g putative ABC transporter {Arabidopsis
thaliana}, partial (13%)
Length = 523
Score = 91.7 bits (226), Expect = 9e-20
Identities = 46/55 (83%), Positives = 50/55 (90%)
Frame = +3
Query: 80 KADLVAVLQQGSVSEIGTHDELCSKGENGVYANLVKMQEMAHETAMNNARKSSAR 134
KADLVAV+QQGSV EI THDEL SKGE+G YA L+KMQE+AHETAMNNARKSSAR
Sbjct: 3 KADLVAVIQQGSVFEIVTHDELFSKGEDGFYAKLIKMQEVAHETAMNNARKSSAR 167
>TC90210 similar to GP|14715462|dbj|BAB62040. CjMDR1 {Coptis japonica},
partial (26%)
Length = 1158
Score = 79.3 bits (194), Expect(2) = 7e-17
Identities = 37/71 (52%), Positives = 53/71 (74%)
Frame = +2
Query: 53 KAVQEALDRFMIGRTTLVIAHRLSTIRKADLVAVLQQGSVSEIGTHDELCSKGENGVYAN 112
+ VQEALDR M+ RTT+V+AHRLST+R AD++AV+ +G + E GTH EL K G Y+
Sbjct: 152 RVVQEALDRIMVNRTTVVVAHRLSTVRNADMIAVIHRGKMVEKGTHSELL-KDPEGAYSQ 328
Query: 113 LVKMQEMAHET 123
L+++QE+ E+
Sbjct: 329 LIRLQEVNKES 361
Score = 23.1 bits (48), Expect(2) = 7e-17
Identities = 10/32 (31%), Positives = 18/32 (56%)
Frame = +1
Query: 15 SATLWKAKTENSDSKGNAEKTSNSSPR*GNKC 46
++T+W + + D K N ++ NS+ * KC
Sbjct: 37 NSTVWWTEAAHCDCKSNP*ESKNSTS**SYKC 132
>TC79211 similar to GP|4204793|gb|AAD10836.1| P-glycoprotein {Solanum
tuberosum}, partial (36%)
Length = 1667
Score = 81.3 bits (199), Expect = 1e-16
Identities = 35/81 (43%), Positives = 57/81 (70%)
Frame = +1
Query: 53 KAVQEALDRFMIGRTTLVIAHRLSTIRKADLVAVLQQGSVSEIGTHDELCSKGENGVYAN 112
++VQEALDR G+TT+++AHRLSTIR A+++AV+ G V+E G+H +L ++G+YA
Sbjct: 1207 RSVQEALDRASTGKTTIIVAHRLSTIRNANVIAVIDDGKVAEQGSHSQLMKNHQDGIYAR 1386
Query: 113 LVKMQEMAHETAMNNARKSSA 133
++++Q H + A SS+
Sbjct: 1387 MIQLQRFTHNEVIGMASGSSS 1449
>TC78215 similar to GP|10177549|dbj|BAB10828. ABC transporter-like protein
{Arabidopsis thaliana}, partial (36%)
Length = 1176
Score = 72.8 bits (177), Expect = 4e-14
Identities = 39/69 (56%), Positives = 47/69 (67%)
Frame = +1
Query: 55 VQEALDRFMIGRTTLVIAHRLSTIRKADLVAVLQQGSVSEIGTHDELCSKGENGVYANLV 114
VQ+A+D M GRT LVIAHRLST++ A+ VAV+ G + E GTHDEL K NGVY LV
Sbjct: 529 VQDAMDSIMKGRTVLVIAHRLSTVKTANTVAVVSDGQIVESGTHDELLEK--NGVYTALV 702
Query: 115 KMQEMAHET 123
K Q +T
Sbjct: 703 KRQLQTTKT 729
>TC90288 weakly similar to GP|21645421|gb|AAF58271.2 CG8523-PA {Drosophila
melanogaster}, partial (4%)
Length = 570
Score = 72.0 bits (175), Expect = 7e-14
Identities = 35/63 (55%), Positives = 47/63 (74%)
Frame = +3
Query: 54 AVQEALDRFMIGRTTLVIAHRLSTIRKADLVAVLQQGSVSEIGTHDELCSKGENGVYANL 113
AVQ A+D+ GRTT+VIAHR++T++ AD + VL+ GSV+EIG H +L SK G Y NL
Sbjct: 3 AVQRAIDKISAGRTTIVIAHRIATVKNADSIVVLEHGSVTEIGDHRQLMSKA--GTYFNL 176
Query: 114 VKM 116
VK+
Sbjct: 177 VKL 185
>TC77937 similar to GP|14715462|dbj|BAB62040. CjMDR1 {Coptis japonica},
partial (24%)
Length = 1164
Score = 71.6 bits (174), Expect = 1e-13
Identities = 36/68 (52%), Positives = 50/68 (72%)
Frame = +3
Query: 53 KAVQEALDRFMIGRTTLVIAHRLSTIRKADLVAVLQQGSVSEIGTHDELCSKGENGVYAN 112
K VQ+ALDR M+ RTT+++AHRLSTI+ ADL+AV++ G ++E G H+ L KG G YA+
Sbjct: 777 KVVQDALDRVMVERTTIIVAHRLSTIKGADLIAVVKNGVIAEKGKHEALLHKG--GDYAS 950
Query: 113 LVKMQEMA 120
LV + A
Sbjct: 951 LVALHTSA 974
>TC77935 similar to PIR|E85023|E85023 probable P-glycoprotein-like protein
[imported] - Arabidopsis thaliana, partial (12%)
Length = 869
Score = 70.9 bits (172), Expect = 2e-13
Identities = 35/64 (54%), Positives = 49/64 (75%)
Frame = +2
Query: 53 KAVQEALDRFMIGRTTLVIAHRLSTIRKADLVAVLQQGSVSEIGTHDELCSKGENGVYAN 112
K VQ+ALDR M+ RTT+++AHRLSTI+ ADL+AV++ G ++E G H+ L KG G YA+
Sbjct: 332 KVVQDALDRVMVERTTIIVAHRLSTIKGADLIAVVKNGVIAEKGKHEALLHKG--GDYAS 505
Query: 113 LVKM 116
LV +
Sbjct: 506 LVAL 517
>TC79335 similar to GP|14715462|dbj|BAB62040. CjMDR1 {Coptis japonica},
partial (23%)
Length = 1135
Score = 67.0 bits (162), Expect(2) = 4e-13
Identities = 32/68 (47%), Positives = 51/68 (74%)
Frame = +2
Query: 53 KAVQEALDRFMIGRTTLVIAHRLSTIRKADLVAVLQQGSVSEIGTHDELCSKGENGVYAN 112
+ VQ+ALD+ M+ RTT+V+AHRLSTI+ AD++AV++ G + E G H+ L + ++G YA+
Sbjct: 698 RVVQDALDKVMVNRTTVVVAHRLSTIKNADVIAVVKNGVIVEKGRHETLINV-KDGFYAS 874
Query: 113 LVKMQEMA 120
LV++ A
Sbjct: 875 LVQLHTSA 898
Score = 22.7 bits (47), Expect(2) = 4e-13
Identities = 12/46 (26%), Positives = 25/46 (54%)
Frame = +1
Query: 14 KSATLWKAKTENSDSKGNAEKTSNSSPR*GNKCIGL*VRKAVQEAL 59
+++T+ A+T+ N +K+ + *GNKCI +R++ +
Sbjct: 580 RNSTIRWAETKGGYCTCNNQKSKDFVT**GNKCIRCRIRESCSRCI 717
>TC81811 similar to GP|6671365|gb|AAF23176.1| P-glycoprotein {Gossypium
hirsutum}, partial (17%)
Length = 850
Score = 69.3 bits (168), Expect = 5e-13
Identities = 35/75 (46%), Positives = 51/75 (67%)
Frame = +1
Query: 53 KAVQEALDRFMIGRTTLVIAHRLSTIRKADLVAVLQQGSVSEIGTHDELCSKGENGVYAN 112
+ VQ+ALDR M RTT+++AHRLSTIR AD ++VLQ G + E GTH L + ++G Y
Sbjct: 451 RIVQQALDRLMQNRTTVMVAHRLSTIRNADQISVLQDGKIIEQGTHSSLI-ENKDGPYYK 627
Query: 113 LVKMQEMAHETAMNN 127
LV +Q+ + + +N
Sbjct: 628 LVNLQQQQNHQS*HN 672
>BG646029 similar to GP|14715462|d CjMDR1 {Coptis japonica}, partial (16%)
Length = 680
Score = 63.5 bits (153), Expect(2) = 8e-13
Identities = 32/59 (54%), Positives = 43/59 (72%)
Frame = -3
Query: 53 KAVQEALDRFMIGRTTLVIAHRLSTIRKADLVAVLQQGSVSEIGTHDELCSKGENGVYA 111
K VQ+ALDR + RTT+V+AHRLSTI+ A+ +AV++ G + E G HD L +KG G YA
Sbjct: 213 KVVQDALDRVRVDRTTIVVAHRLSTIKGANSIAVVKNGVIEEKGKHDILINKG--GTYA 43
Score = 25.0 bits (53), Expect(2) = 8e-13
Identities = 9/33 (27%), Positives = 19/33 (57%)
Frame = -2
Query: 14 KSATLWKAKTENSDSKGNAEKTSNSSPR*GNKC 46
+ +T W+ + + K ++ + N + R*G+KC
Sbjct: 331 RDSTFWRTEATCGNCKSHS*QAKNITTR*GHKC 233
>TC83850 weakly similar to GP|7300071|gb|AAF55241.1| CG4225 gene product
{Drosophila melanogaster}, partial (23%)
Length = 816
Score = 56.2 bits (134), Expect = 4e-09
Identities = 26/53 (49%), Positives = 36/53 (67%)
Frame = +2
Query: 53 KAVQEALDRFMIGRTTLVIAHRLSTIRKADLVAVLQQGSVSEIGTHDELCSKG 105
+ +Q+AL+R GRTT+ IAHRLSTI +D + VL + + E GTH EL + G
Sbjct: 497 RQIQDALERVTAGRTTITIAHRLSTITTSDQIVVLHKSKIVERGTHSELLALG 655
>TC80024 homologue to PIR|T47671|T47671 P-glycoprotein-like - Arabidopsis
thaliana, partial (8%)
Length = 1021
Score = 55.5 bits (132), Expect = 7e-09
Identities = 29/64 (45%), Positives = 43/64 (66%), Gaps = 1/64 (1%)
Frame = +3
Query: 53 KAVQEALDRFMIG-RTTLVIAHRLSTIRKADLVAVLQQGSVSEIGTHDELCSKGENGVYA 111
+ VQEALD ++G +TT++IAHR + +R D + VL G + E GTHD L +K NG+Y
Sbjct: 129 RVVQEALDTLIMGNKTTILIAHRAAMMRHVDNIVVLNGGRIVEEGTHDSLVAK--NGLYV 302
Query: 112 NLVK 115
L++
Sbjct: 303 RLMQ 314
>BM780003 weakly similar to PIR|T06165|T0 multidrug resistance protein 1
homolog - barley, partial (12%)
Length = 665
Score = 54.3 bits (129), Expect = 2e-08
Identities = 27/48 (56%), Positives = 35/48 (72%)
Frame = +2
Query: 70 VIAHRLSTIRKADLVAVLQQGSVSEIGTHDELCSKGENGVYANLVKMQ 117
V+AH+LSTIR ADL+AV+ G + E GTH+EL + NG YA L K+Q
Sbjct: 2 VVAHKLSTIRNADLIAVVSNGCIIESGTHNELINT-PNGHYAKLAKLQ 142
>BQ751194 similar to GP|21622336|em probable multidrug resistance protein 2
{Neurospora crassa}, partial (16%)
Length = 808
Score = 46.6 bits (109), Expect = 3e-06
Identities = 29/65 (44%), Positives = 42/65 (64%), Gaps = 2/65 (3%)
Frame = -2
Query: 55 VQEALDRFMIGR-TTLVIAHRLSTIRKADLVAVL-QQGSVSEIGTHDELCSKGENGVYAN 112
V AL + GR TT+ IAHRLSTI+++DL+ VL G V+EIG + EL S + ++
Sbjct: 609 VNAALASLLKGRNTTISIAHRLSTIKRSDLIIVLSNDGKVAEIGKYSEL-SANKESAFSK 433
Query: 113 LVKMQ 117
L++ Q
Sbjct: 432 LMEWQ 418
>TC89340 weakly similar to GP|17427488|emb|CAD14006. PROBABLE COMPOSITE
ATP-BINDING TRANSMEMBRANE ABC TRANSPORTER PROTEIN
{Ralstonia solanacearum}, partial (32%)
Length = 795
Score = 39.7 bits (91), Expect = 4e-04
Identities = 23/47 (48%), Positives = 27/47 (56%)
Frame = +1
Query: 55 VQEALDRFMIGRTTLVIAHRLSTIRKADLVAVLQQGSVSEIGTHDEL 101
+Q+ L GRT L+IAHRLSTI AD + V QQ G H EL
Sbjct: 631 IQDRLGHLGQGRTLLIIAHRLSTITHADQIIVPQQWDY**KGHHKEL 771
>TC80066 similar to PIR|T50518|T50518 ABC transporter-like protein -
Arabidopsis thaliana, partial (14%)
Length = 992
Score = 37.4 bits (85), Expect = 0.002
Identities = 18/67 (26%), Positives = 35/67 (51%)
Frame = +3
Query: 55 VQEALDRFMIGRTTLVIAHRLSTIRKADLVAVLQQGSVSEIGTHDELCSKGENGVYANLV 114
+Q+ + + RT + IAHR+ T+ +DLV VL G ++E +L + ++ Y +
Sbjct: 252 IQDIICQQFNNRTVVTIAHRIHTVIDSDLVLVLSDGRIAEYDEPSKLLEREDSFFYKLIK 431
Query: 115 KMQEMAH 121
+ +H
Sbjct: 432 EYSSRSH 452
>AJ497912 similar to GP|8777328|dbj| ABC transporter-like protein
{Arabidopsis thaliana}, partial (8%)
Length = 342
Score = 34.3 bits (77), Expect = 0.017
Identities = 18/46 (39%), Positives = 27/46 (58%)
Frame = +2
Query: 68 TLVIAHRLSTIRKADLVAVLQQGSVSEIGTHDELCSKGENGVYANL 113
++ IAHRL+T + D + VL+ G V E G H+ L + G Y+ L
Sbjct: 2 SIFIAHRLTTAMQCDKIIVLENGKVVEHGPHEVLLANA--GRYSQL 133
>TC87489 homologue to PIR|T52080|T52080 multi resistance protein [imported]
- Arabidopsis thaliana, partial (5%)
Length = 1033
Score = 31.6 bits (70), Expect = 0.11
Identities = 15/31 (48%), Positives = 19/31 (60%)
Frame = +3
Query: 67 TTLVIAHRLSTIRKADLVAVLQQGSVSEIGT 97
T IAHR+ T+ +DLV VL G V+E T
Sbjct: 174 TVCTIAHRIPTVIDSDLVLVLSDGLVAEFDT 266
>TC87490 similar to PIR|T52080|T52080 multi resistance protein [imported] -
Arabidopsis thaliana, partial (19%)
Length = 927
Score = 30.4 bits (67), Expect = 0.25
Identities = 14/31 (45%), Positives = 19/31 (61%)
Frame = +1
Query: 67 TTLVIAHRLSTIRKADLVAVLQQGSVSEIGT 97
T IAHR+ T+ +DLV VL G ++E T
Sbjct: 727 TVCTIAHRIPTVIDSDLVLVLNDGLLAEFDT 819
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.335 0.142 0.425
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,498,704
Number of Sequences: 36976
Number of extensions: 32359
Number of successful extensions: 202
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 200
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 200
length of query: 142
length of database: 9,014,727
effective HSP length: 87
effective length of query: 55
effective length of database: 5,797,815
effective search space: 318879825
effective search space used: 318879825
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.7 bits)
S2: 53 (25.0 bits)
Lotus: description of TM0346.4