BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0346.3
(374 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BE239758 similar to PIR|A84564|A845 hypothetical protein At2g184... 216 9e-57
BG644405 weakly similar to PIR|A84564|A845 hypothetical protein ... 177 6e-45
TC77681 similar to GP|22597156|gb|AAN03465.1 nucleolar histone d... 33 0.18
TC77337 weakly similar to GP|4019275|gb|AAC95573.1| orf 48 {Atel... 31 0.68
TC80981 similar to GP|8698730|gb|AAF78488.1| ESTs gb|Z18526 gb|... 31 0.88
CA989870 similar to GP|18201934|g kinesin-like protein heavy cha... 31 0.88
TC87688 similar to GP|10177535|dbj|BAB10930. gene_id:K1F13.21~un... 30 1.5
TC93070 weakly similar to GP|17065230|gb|AAL32769.1 Unknown prot... 29 3.4
TC80287 weakly similar to GP|12667516|gb|AAG61157.1 DBC2 {Homo s... 29 3.4
TC77099 homologue to PIR|T14304|T14304 ribosomal protein - carro... 28 4.4
TC77098 homologue to PIR|T14304|T14304 ribosomal protein - carro... 28 4.4
TC77517 similar to PIR|T51159|T51159 HMG protein [imported] - Ar... 28 5.7
TC84342 similar to GP|20805268|dbj|BAB92934. putative uncharacte... 28 7.5
TC80763 similar to GP|13926247|gb|AAK49597.1 AT5g16950/F2K13_100... 28 7.5
TC86965 similar to GP|22022558|gb|AAM83236.1 At5g12410 {Arabidop... 27 9.8
BQ146505 similar to PIR|C96598|C96 hypothetical protein T5A14.2 ... 27 9.8
TC78227 similar to GP|10176883|dbj|BAB10113. contains similarity... 27 9.8
>BE239758 similar to PIR|A84564|A845 hypothetical protein At2g18410
[imported] - Arabidopsis thaliana, partial (16%)
Length = 484
Score = 216 bits (551), Expect = 9e-57
Identities = 104/123 (84%), Positives = 115/123 (92%)
Frame = +3
Query: 252 NGRVRVTCEEFKVEAGGISLTSVSSADGATVAGLLPKVQFNLQLSEKEKTDRAKVVLPFE 311
NGRVRVTCE+FKVEAGGIS TSVS+ DG TVAGLLPKVQFNLQLSEKE+ DRAKVVLPFE
Sbjct: 3 NGRVRVTCEKFKVEAGGISFTSVSAEDGTTVAGLLPKVQFNLQLSEKEQVDRAKVVLPFE 182
Query: 312 HQGTGRPIQIYDGRRSLDDNSSEAAPISSGKKENSEMGEIIYFRDSDDEMPDSDEDPDDD 371
HQG G PIQIYDGRRSL+++SS+ AP+S GKKE+S++GEIIYFRDSDDEMPDSDEDPDDD
Sbjct: 183 HQGNGAPIQIYDGRRSLEESSSDGAPLSKGKKEDSDLGEIIYFRDSDDEMPDSDEDPDDD 362
Query: 372 LDI 374
LDI
Sbjct: 363 LDI 371
>BG644405 weakly similar to PIR|A84564|A845 hypothetical protein At2g18410
[imported] - Arabidopsis thaliana, partial (8%)
Length = 603
Score = 177 bits (449), Expect = 6e-45
Identities = 96/189 (50%), Positives = 128/189 (66%)
Frame = +2
Query: 28 LTSPLASDVFSHLLLQLSSHLIASKSHSQFRSGIVIVALSRTPSSYAALFKTKGIHISSS 87
L+ P +S +F LL S + + G+V+VALSR PS YA L K KG +SSS
Sbjct: 8 LSVPSSSTIFLRNLLPTFSPV------NHKLEGVVLVALSRPPSFYAELLKDKGFDVSSS 169
Query: 88 NNNWIHILDCYTDPLGWKEKPRKSQNVTNPSHQVSLATTSYKTVKDLDKLFSVITELGRG 147
+ W+ +LDCY+DPLGWK+K + V NP + SL T+ K +K+LDK+ S I ELG+
Sbjct: 170 SK-WLRVLDCYSDPLGWKKKLMEKGTVRNPYEETSLKTSLCKNLKELDKVLSSIIELGKE 346
Query: 148 LIGENKARFCVAIDSLSELLRHASLQSVAGLLSNLRSHDQISSIFGLLHSDLHEERAAAV 207
++ + K RF VAIDS+SE+LRH+SL SVA +LS+LRSHDQ+S IF L H DLHE + AA
Sbjct: 347 IVEDGKGRFAVAIDSVSEVLRHSSLPSVARILSHLRSHDQVSCIFCLWHVDLHEAKVAAT 526
Query: 208 LEYMSSMVA 216
LEY+S+M A
Sbjct: 527 LEYLSTMHA 553
>TC77681 similar to GP|22597156|gb|AAN03465.1 nucleolar histone deacetylase
HD2-P39 {Glycine max}, partial (47%)
Length = 1233
Score = 33.1 bits (74), Expect = 0.18
Identities = 27/89 (30%), Positives = 44/89 (49%), Gaps = 2/89 (2%)
Frame = +2
Query: 287 PKVQFNLQLSEKEKTDRAKVVLPFEHQGTGRPIQIYDGRRSLDDNSSEAAPISSGKKENS 346
PK + L++SE +K D AK P ++ T + +++ D + D+ E+ +
Sbjct: 458 PKAE-ELKVSEPKKAD-AKNAAPAKNAATAKHVKVVDPKDEDSDDDDES---------DD 604
Query: 347 EMGEIIYFRDSDDEMP--DSDEDPDDDLD 373
E+G SDDEM DSD + +DD D
Sbjct: 605 EIGS------SDDEMENADSDSEDEDDSD 673
>TC77337 weakly similar to GP|4019275|gb|AAC95573.1| orf 48 {Ateline
herpesvirus 3}, partial (8%)
Length = 986
Score = 31.2 bits (69), Expect = 0.68
Identities = 19/48 (39%), Positives = 24/48 (49%)
Frame = +3
Query: 324 GRRSLDDNSSEAAPISSGKKENSEMGEIIYFRDSDDEMPDSDEDPDDD 371
G S + ++S+ AP G E GE D DD+ D DED DDD
Sbjct: 519 GNNSNNKSNSKKAP-EGGAGGADENGEEEDDEDGDDQDEDDDEDEDDD 659
>TC80981 similar to GP|8698730|gb|AAF78488.1| ESTs gb|Z18526 gb|AI994480
gb|T44186 gb|Z30806 come from this gene. {Arabidopsis
thaliana}, partial (37%)
Length = 699
Score = 30.8 bits (68), Expect = 0.88
Identities = 15/50 (30%), Positives = 26/50 (52%), Gaps = 5/50 (10%)
Frame = +3
Query: 329 DDNSSEAAPISSGK-----KENSEMGEIIYFRDSDDEMPDSDEDPDDDLD 373
D+ +S+ P+ K +++ G++I D DD+ D +D DDD D
Sbjct: 276 DEENSDEEPVVDRKGKGIMRDDKGKGKMIEEEDEDDDDSDDSDDSDDDSD 425
>CA989870 similar to GP|18201934|g kinesin-like protein heavy chain
{Arabidopsis thaliana}, partial (9%)
Length = 806
Score = 30.8 bits (68), Expect = 0.88
Identities = 19/64 (29%), Positives = 31/64 (47%), Gaps = 2/64 (3%)
Frame = +2
Query: 300 KTDRAKVVLPFE--HQGTGRPIQIYDGRRSLDDNSSEAAPISSGKKENSEMGEIIYFRDS 357
++ + + V PF T ++ + +RS+DD SSEA SSGK+ +DS
Sbjct: 296 ESQKPRAVSPFRMPKYSTSGSMKPENSQRSMDDRSSEAKNYSSGKQRRPRFPSTFMDKDS 475
Query: 358 DDEM 361
+M
Sbjct: 476 IPKM 487
>TC87688 similar to GP|10177535|dbj|BAB10930. gene_id:K1F13.21~unknown
protein {Arabidopsis thaliana}, partial (46%)
Length = 2077
Score = 30.0 bits (66), Expect = 1.5
Identities = 23/84 (27%), Positives = 35/84 (41%)
Frame = +2
Query: 290 QFNLQLSEKEKTDRAKVVLPFEHQGTGRPIQIYDGRRSLDDNSSEAAPISSGKKENSEMG 349
QF + L +K ++ K V E + D LDD+ + + K + G
Sbjct: 314 QFKVPLDVGKKLEKKKRVELEEEESD-------DFDEELDDDDDDFEGVEKKKAKGGSEG 472
Query: 350 EIIYFRDSDDEMPDSDEDPDDDLD 373
E F + DDE + ED DD+ D
Sbjct: 473 EDD-FEEEDDEDEEGSEDEDDEED 541
>TC93070 weakly similar to GP|17065230|gb|AAL32769.1 Unknown protein
{Arabidopsis thaliana}, partial (19%)
Length = 679
Score = 28.9 bits (63), Expect = 3.4
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +1
Query: 354 FRDSDDEMPDSDEDPDDDLD 373
F D +DE D DED DDD D
Sbjct: 250 FEDDEDEDEDEDEDDDDDDD 309
>TC80287 weakly similar to GP|12667516|gb|AAG61157.1 DBC2 {Homo sapiens},
partial (4%)
Length = 779
Score = 28.9 bits (63), Expect = 3.4
Identities = 21/59 (35%), Positives = 28/59 (46%), Gaps = 1/59 (1%)
Frame = -3
Query: 316 GRPIQIYDGRR-SLDDNSSEAAPISSGKKENSEMGEIIYFRDSDDEMPDSDEDPDDDLD 373
G PI++ RR LDD G E E + D DD+ +S++D DDDLD
Sbjct: 537 GDPIELLTHRRIHLDDPVENHHHHHVGYSE-----EQVNHDDDDDDDDESEDDGDDDLD 376
>TC77099 homologue to PIR|T14304|T14304 ribosomal protein - carrot
(fragment), partial (84%)
Length = 1116
Score = 28.5 bits (62), Expect = 4.4
Identities = 24/78 (30%), Positives = 36/78 (45%), Gaps = 1/78 (1%)
Frame = -2
Query: 22 LTITDSLTSPLASDVFSHLLLQLSSHLIASK-SHSQFRSGIVIVALSRTPSSYAALFKTK 80
L I+ S TSP + HLL L L+ SK S + +V L + SY L + K
Sbjct: 890 LLISCSTTSPHLPEDIRHLLSLLLCTLVGSKLSLCNLKRPLVFSNLEQL--SYTLLIRCK 717
Query: 81 GIHISSSNNNWIHILDCY 98
++S + +H L C+
Sbjct: 716 PSNLSDEVPHKVHPLACF 663
>TC77098 homologue to PIR|T14304|T14304 ribosomal protein - carrot
(fragment), partial (84%)
Length = 616
Score = 28.5 bits (62), Expect = 4.4
Identities = 24/78 (30%), Positives = 36/78 (45%), Gaps = 1/78 (1%)
Frame = -1
Query: 22 LTITDSLTSPLASDVFSHLLLQLSSHLIASK-SHSQFRSGIVIVALSRTPSSYAALFKTK 80
L I+ S TSP + HLL L L+ SK S + +V L + SY L + K
Sbjct: 412 LLISCSTTSPHLPEDIRHLLSLLLCTLVGSKLSLCNLKRPLVFSNLEQL--SYTLLIRCK 239
Query: 81 GIHISSSNNNWIHILDCY 98
++S + +H L C+
Sbjct: 238 PSNLSDEVPHKVHPLACF 185
>TC77517 similar to PIR|T51159|T51159 HMG protein [imported] - Arabidopsis
thaliana, partial (70%)
Length = 907
Score = 28.1 bits (61), Expect = 5.7
Identities = 20/86 (23%), Positives = 39/86 (45%), Gaps = 8/86 (9%)
Frame = +3
Query: 297 EKEKTDRAKVVLPFEHQGTGRPIQI------YDGRRSLDDNSSEAAPISSGKKENSE--M 348
EK K+ P+E + R ++ Y+ + S D+ E + + + N +
Sbjct: 384 EKWKSLTKAEKAPYEAKAAKRKVEYEKLMNAYNNKPSSADDDEEESDKDNSEVNNEDEAS 563
Query: 349 GEIIYFRDSDDEMPDSDEDPDDDLDI 374
GE + D +++ D D+D DD+ D+
Sbjct: 564 GEDDHQDDEEEDDEDEDDDEDDE*DL 641
>TC84342 similar to GP|20805268|dbj|BAB92934. putative uncharacterized
hypothalamus protein HT010 {Oryza sativa (japonica
cultivar-group)}, partial (26%)
Length = 795
Score = 27.7 bits (60), Expect = 7.5
Identities = 11/19 (57%), Positives = 16/19 (83%), Gaps = 1/19 (5%)
Frame = +3
Query: 357 SDDEMPDS-DEDPDDDLDI 374
+DDE+PD D+D DD+LD+
Sbjct: 357 ADDELPDDIDDDDDDELDL 413
>TC80763 similar to GP|13926247|gb|AAK49597.1 AT5g16950/F2K13_100
{Arabidopsis thaliana}, partial (28%)
Length = 998
Score = 27.7 bits (60), Expect = 7.5
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = -1
Query: 305 KVVLPFEHQGTGRPIQIYDGRRSLDDN 331
K+V+P HQGT ++ + GRR L N
Sbjct: 371 KLVIPATHQGTSVLLRAFSGRRRLFTN 291
>TC86965 similar to GP|22022558|gb|AAM83236.1 At5g12410 {Arabidopsis
thaliana}, partial (59%)
Length = 1455
Score = 27.3 bits (59), Expect = 9.8
Identities = 17/64 (26%), Positives = 28/64 (43%)
Frame = +1
Query: 311 EHQGTGRPIQIYDGRRSLDDNSSEAAPISSGKKENSEMGEIIYFRDSDDEMPDSDEDPDD 370
EHQ T + I D + + + A S K + + + I F DSD D DE+ ++
Sbjct: 199 EHQATREALNILD---NFYEELIDGAHPSVTKLSDKPLNKKITFADSDSSSIDDDEEEEE 369
Query: 371 DLDI 374
+
Sbjct: 370 KAQV 381
>BQ146505 similar to PIR|C96598|C96 hypothetical protein T5A14.2 [imported] -
Arabidopsis thaliana, partial (38%)
Length = 649
Score = 27.3 bits (59), Expect = 9.8
Identities = 19/59 (32%), Positives = 28/59 (47%), Gaps = 8/59 (13%)
Frame = +3
Query: 14 LEGELAPTLTITDSLTSPLASDVFSHLLLQLSSHLI--------ASKSHSQFRSGIVIV 64
L G + LTITD TSP+ S + LL + S +I K HS + +V++
Sbjct: 354 LLGPILKVLTITDRSTSPVPSSSLTILLTLMESFVILLTEFPTRTMKPHSSLLNTMVLL 530
>TC78227 similar to GP|10176883|dbj|BAB10113. contains similarity to unknown
protein~gb|AAD10691.1~gene_id:K9L2.3 {Arabidopsis
thaliana}, partial (27%)
Length = 1707
Score = 27.3 bits (59), Expect = 9.8
Identities = 14/54 (25%), Positives = 24/54 (43%)
Frame = +1
Query: 318 PIQIYDGRRSLDDNSSEAAPISSGKKENSEMGEIIYFRDSDDEMPDSDEDPDDD 371
P Q +L++N I++ + N E+ ++E D +ED DDD
Sbjct: 115 PAQKRSFEEALEENEESENEINNTSQPNDELESDQRHSSENEEDEDEEEDEDDD 276
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.314 0.131 0.362
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,318,607
Number of Sequences: 36976
Number of extensions: 136760
Number of successful extensions: 878
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 758
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 824
length of query: 374
length of database: 9,014,727
effective HSP length: 98
effective length of query: 276
effective length of database: 5,391,079
effective search space: 1487937804
effective search space used: 1487937804
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0346.3