BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0344.4
(242 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC80788 similar to GP|16417948|gb|AAL18926.1 phosphomevalonate k... 29 1.9
TC92898 similar to GP|9714052|emb|CAC01296.1 sterol C-14 reducta... 28 3.2
TC92942 similar to GP|15451042|gb|AAK96792.1 putative protein {A... 27 5.5
TC85643 similar to GP|4530585|gb|AAD22104.1| B12D protein {Ipomo... 27 5.5
AL384864 27 5.5
TC76789 similar to GP|15294254|gb|AAK95304.1 AT4g24770/F22K18_30... 27 7.1
TC88474 similar to GP|19698365|gb|AAL93108.1 holocarboxylase syn... 27 7.1
AW689742 similar to PIR|T48216|T48 hypothetical protein T20L15.2... 27 7.1
TC77653 similar to GP|20334820|gb|AAM16166.1 At1g12050/F12F1_8 {... 27 9.3
>TC80788 similar to GP|16417948|gb|AAL18926.1 phosphomevalonate kinase
{Hevea brasiliensis}, partial (32%)
Length = 795
Score = 28.9 bits (63), Expect = 1.9
Identities = 15/43 (34%), Positives = 21/43 (47%)
Frame = -2
Query: 109 ICLRDKRMLRFHESVLAVLECNIQNGPAYFNCFPSYPVCLRDP 151
I L D RML+ + L C+ N F+C + +CLR P
Sbjct: 185 IHLSDLRMLQLSITDLYASHCSFANFLNKFSCISNAEICLRQP 57
>TC92898 similar to GP|9714052|emb|CAC01296.1 sterol C-14 reductase (FACKEL)
{Arabidopsis thaliana}, partial (55%)
Length = 689
Score = 28.1 bits (61), Expect = 3.2
Identities = 12/46 (26%), Positives = 26/46 (56%), Gaps = 2/46 (4%)
Frame = -1
Query: 108 LICLRDKRMLRFHESVLAVLECNIQNGPAY--FNCFPSYPVCLRDP 151
++ R R++ H + A+ + N+++ P F+C P++ C+R P
Sbjct: 518 MLLARTDRLISSHPIIPALTKKNLRSIPMN*GFSCIPNHQSCIRSP 381
>TC92942 similar to GP|15451042|gb|AAK96792.1 putative protein {Arabidopsis
thaliana}, partial (38%)
Length = 650
Score = 27.3 bits (59), Expect = 5.5
Identities = 14/40 (35%), Positives = 19/40 (47%), Gaps = 2/40 (5%)
Frame = -1
Query: 131 IQNGPAYFNCFPS--YPVCLRDPHVTTCVDLDIKIPNEMF 168
+ N P FNCF S +P C P + TC ++ P F
Sbjct: 347 LANSPDSFNCFKSSAFPTCF--PLIKTCGNVTTDFPPSAF 234
>TC85643 similar to GP|4530585|gb|AAD22104.1| B12D protein {Ipomoea
batatas}, complete
Length = 831
Score = 27.3 bits (59), Expect = 5.5
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = +3
Query: 137 YFNCFPSYPVCLRDPHVTTCV 157
+FNC+P P+ DP T C+
Sbjct: 141 FFNCYPWPPIAGSDPRYTLCL 203
>AL384864
Length = 477
Score = 27.3 bits (59), Expect = 5.5
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +1
Query: 52 KTVEKSFAVQGPYQRISILDSRIVNQHVRKYRD 84
K+++ F V + I+ + S I NQH+R Y+D
Sbjct: 205 KSIKTQFNVSSTNRLITNVKSYISNQHLRSYKD 303
>TC76789 similar to GP|15294254|gb|AAK95304.1 AT4g24770/F22K18_30
{Arabidopsis thaliana}, partial (62%)
Length = 1294
Score = 26.9 bits (58), Expect = 7.1
Identities = 12/35 (34%), Positives = 21/35 (59%)
Frame = -2
Query: 12 NSEENDSNLEQKLQNWSIPKIRTNQVYKTSIFEFN 46
NS++ SNL++ LQNW + Q+ + F+F+
Sbjct: 474 NSQQKSSNLQKVLQNWQL------QLLHSRYFQFH 388
>TC88474 similar to GP|19698365|gb|AAL93108.1 holocarboxylase synthetase 1
{Arabidopsis thaliana}, partial (44%)
Length = 818
Score = 26.9 bits (58), Expect = 7.1
Identities = 11/35 (31%), Positives = 20/35 (56%)
Frame = +3
Query: 150 DPHVTTCVDLDIKIPNEMFIDGIPPVSIMYRFCYK 184
D + CVD+ IK PN+++++G I+ Y+
Sbjct: 636 DKNGLPCVDVKIKWPNDLYLNGFKVGGILCTSTYR 740
>AW689742 similar to PIR|T48216|T48 hypothetical protein T20L15.220 -
Arabidopsis thaliana, partial (13%)
Length = 674
Score = 26.9 bits (58), Expect = 7.1
Identities = 15/61 (24%), Positives = 31/61 (50%), Gaps = 1/61 (1%)
Frame = +2
Query: 157 VDLDIKIPNEMFIDGIPPVSIMYRFCYKVTDNLSGIKAVRDYTVNE-TEVTEINAENSSR 215
+DLD + +++F D PP+++ R S I+ + + +E +V E + NS+
Sbjct: 38 IDLDNNLLSDIFGDLNPPINVTLRLSGNPVCKKSNIQGIGQFCAHERRDVDESESTNSTD 217
Query: 216 I 216
+
Sbjct: 218 V 220
>TC77653 similar to GP|20334820|gb|AAM16166.1 At1g12050/F12F1_8 {Arabidopsis
thaliana}, partial (36%)
Length = 778
Score = 26.6 bits (57), Expect = 9.3
Identities = 16/53 (30%), Positives = 29/53 (54%), Gaps = 5/53 (9%)
Frame = +3
Query: 193 KAVRDYTVN-ETEVTEINAENSSRITRKILKHDEITYPQE----WLQGCSSQP 240
K ++Y ++ E + +N ++SS +TR LKH T Q+ + GC+ +P
Sbjct: 87 KVSKNYDLSLEVHIKPVNHKDSSIVTRSNLKHLYWTLTQQLAHHTINGCNLRP 245
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.321 0.137 0.415
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,432,146
Number of Sequences: 36976
Number of extensions: 126425
Number of successful extensions: 525
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 523
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 525
length of query: 242
length of database: 9,014,727
effective HSP length: 93
effective length of query: 149
effective length of database: 5,575,959
effective search space: 830817891
effective search space used: 830817891
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0344.4