BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0343.3
(521 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC76734 similar to PIR|T06468|T06468 pectinesterase (EC 3.1.1.11... 394 e-110
TC76733 similar to PIR|T06468|T06468 pectinesterase (EC 3.1.1.11... 392 e-109
TC76732 homologue to PIR|T06468|T06468 pectinesterase (EC 3.1.1.... 392 e-109
TC85623 similar to GP|13605696|gb|AAK32841.1 At2g45220/F4L23.27 ... 374 e-104
TC87503 similar to SP|Q43111|PME3_PHAVU Pectinesterase 3 precurs... 353 9e-98
TC86464 similar to GP|2098711|gb|AAB57670.1| pectinesterase {Cit... 350 8e-97
TC86403 similar to PIR|B86158|B86158 hypothetical protein AAF028... 343 9e-95
TC77735 similar to GP|14334646|gb|AAK59501.1 putative pectineste... 343 1e-94
TC77013 similar to GP|7025485|gb|AAF35897.1| pectin methylestera... 329 2e-90
NP212791 NP212791|AJ249611.1|CAB65291.1 pectin methyl-esterase PEF1 280 7e-76
TC89457 weakly similar to SP|Q43062|PME_PRUPE Pectinesterase PPE... 253 1e-67
TC91752 similar to GP|6630559|gb|AAF19578.1| putative pectineste... 242 3e-64
BE319906 similar to SP|Q43062|PME_ Pectinesterase PPE8B precurso... 239 2e-63
BQ138825 similar to PIR|T49922|T49 pectin methylesterase-like pr... 201 4e-52
TC89627 weakly similar to GP|17529290|gb|AAL38872.1 putative pec... 146 1e-48
BI272853 weakly similar to PIR|T04359|T04 pectin methylesterase-... 179 3e-45
TC84419 similar to SP|Q42920|PME_MEDSA Pectinesterase precursor ... 168 5e-42
AL368659 similar to PIR|B86158|B86 hypothetical protein AAF02886... 155 3e-38
TC93392 similar to PIR|T49241|T49241 pectinesterase-like protein... 140 2e-33
TC82059 similar to GP|19424045|gb|AAL87311.1 unknown protein {Ar... 125 3e-29
>TC76734 similar to PIR|T06468|T06468 pectinesterase (EC 3.1.1.11) precursor
- garden pea, complete
Length = 1981
Score = 394 bits (1011), Expect = e-110
Identities = 204/452 (45%), Positives = 290/452 (64%), Gaps = 7/452 (1%)
Frame = +3
Query: 75 NDNRLSNAISDCLELFDMAYENLKWSI-SATQNHQGNKNNGTGNLSSDLRTWISAVLSYQ 133
N R NA++ C +L +++ E + S+ + T+++ ++ D TW+S+VL+
Sbjct: 381 NSPREENALNVCEKLMNLSMERVWDSVLTLTKDNMDSQQ--------DAHTWLSSVLTNH 536
Query: 134 ETCIDGFEGTNSNVKDHVSGGLDQVISLVKNEL--LLQVVPDSDPSNI---SNSGEFPSW 188
TC+DG EGT+ V ++ + +I+ ++ L L+ V+P D S +G+FPSW
Sbjct: 537 ATCLDGLEGTSRAVMEN---DIQDLIARARSSLAVLVAVLPPKDHDEFIDESLNGDFPSW 707
Query: 189 VKAEDEKLLQQDAPPPPADVVVAADGSGNYTTVMDAVKDAPEYNMTRYVIHVKKGIYKEY 248
V ++D +LL+ A+VVVA DGSG + TV +AV AP RYVI+VKKGIYKE
Sbjct: 708 VTSKDRRLLESSVGDVKANVVVAKDGSGKFKTVAEAVASAPNKGTARYVIYVKKGIYKEN 887
Query: 249 VEIKKKKWNIMIVGDGMDVTTISGNRSYGHDNITTFRTATFAVSAIGFIARDITFENTAG 308
VEI K N+M++GDGMD T I+G+ +Y D TF+TAT A FIA+DI F+NTAG
Sbjct: 888 VEIASSKTNVMLLGDGMDATIITGSLNYV-DGTGTFQTATVAAVGDWFIAQDIGFQNTAG 1064
Query: 309 PENKQAVALRSDSDLSVFYRCGIKGYQDSLYAHSQRQFYKECRIRGTVDFICGNAAAVFQ 368
P+ QAVALR SD SV RC I +QD+LYAH+ RQFY++ I GT+DFI G+AA V Q
Sbjct: 1065PQKHQAVALRVGSDRSVINRCKIDAFQDTLYAHTNRQFYRDSFITGTIDFIFGDAAVVLQ 1244
Query: 369 NCLIQPKKPLKDQKNTIAAQSRTHPNQTSGFSFHNCTIIPDPDFQPSNGSIQTFLGRPWK 428
C + +KP+ +Q N + AQ R PNQ + S C +IP D +P GS++T+LGRPWK
Sbjct: 1245KCKLVARKPMANQNNMVTAQGRIDPNQNTATSIQQCDVIPSTDLKPVIGSVKTYLGRPWK 1424
Query: 429 NFSRTVFMESFMSEMIHPEGWLEWE-TNTTYDDTLFFGEYQNKGPGSGVANRVKWRGYHV 487
+SRTV M+S + I P GW EW+ + + TL++GEY N GPG+G + RVKW GYH+
Sbjct: 1425KYSRTVVMQSLLGAHIDPTGWAEWDAASKDFLQTLYYGEYMNSGPGAGTSKRVKWPGYHI 1604
Query: 488 LNNSQAMDFTVNQFIKGDLWLPSTGVSYNAGL 519
+N ++A FTV Q I+G++WL +TGV++ AGL
Sbjct: 1605INTAEANKFTVAQLIQGNVWLKNTGVAFIAGL 1700
>TC76733 similar to PIR|T06468|T06468 pectinesterase (EC 3.1.1.11) precursor
- garden pea, partial (98%)
Length = 2029
Score = 392 bits (1008), Expect = e-109
Identities = 211/454 (46%), Positives = 284/454 (62%), Gaps = 9/454 (1%)
Frame = +2
Query: 75 NDNRLSNAISDCLELFDMAYENLKWS--ISATQNHQGNKNNGTGNLSSDLRTWISAVLSY 132
N R A+SDC +L D++ + W + T+N+ ++ D TW+S+VL+
Sbjct: 389 NSPREEIALSDCEQLMDLSMNRI-WDTMLKLTKNNIDSQQ--------DAHTWLSSVLTN 541
Query: 133 QETCIDGFEGTNSNVKDHVSGGLDQVISLVKNEL--LLQVVPDSDPSNISNS---GEFPS 187
TC+DG EG++ V ++ L +IS ++ L L V P D + GEFPS
Sbjct: 542 HATCLDGLEGSSRVVMEN---DLQDLISRARSSLAVFLVVFPQKDRDQFIDETLIGEFPS 712
Query: 188 WVKAEDEKLLQQDAPPPPADVVVAADGSGNYTTVMDAVKDAPEYNMTRYVIHVKKGIYKE 247
WV ++D +LL+ A+VVVA DGSG + TV +AV AP+ T+YVI+VKKG YKE
Sbjct: 713 WVTSKDRRLLETAVGDIKANVVVAQDGSGKFKTVAEAVASAPDNGKTKYVIYVKKGTYKE 892
Query: 248 YVEIKKKKWNIMIVGDGMDVTTISGNRSYGHDNITTFRTATFAVSAIGFIARDITFENTA 307
VEI KK N+M+VGDGMD T I+GN ++ D TTF+++T A GFIA+DI F+N A
Sbjct: 893 NVEIGSKKTNVMLVGDGMDATIITGNLNF-IDGTTTFKSSTVAAVGDGFIAQDIWFQNMA 1069
Query: 308 GPENKQAVALRSDSDLSVFYRCGIKGYQDSLYAHSQRQFYKECRIRGTVDFICGNAAAVF 367
G QAVALR SD SV RC I +QD+LYAHS RQFY++ I GT+DFI GNAA VF
Sbjct: 1070GAAKHQAVALRVGSDQSVINRCRIDAFQDTLYAHSNRQFYRDSVITGTIDFIFGNAAVVF 1249
Query: 368 QNCLIQPKKPLKDQKNTIAAQSRTHPNQTSGFSFHNCTIIPDPDFQPSNGSIQTFLGRPW 427
Q C + +KP+ +Q N AQ R P Q +G S C + P D +P GSI+TFLGRPW
Sbjct: 1250QKCKLVARKPMANQNNMFTAQGREDPGQNTGTSIQQCDLTPSSDLKPVVGSIKTFLGRPW 1429
Query: 428 KNFSRTVFMESFMSEMIHPEGWLEWE-TNTTYDDTLFFGEYQNKGPGSGVANRVKWRGYH 486
K +SRTV M+SF+ I P GW EW+ + + TL++GEY N GPG+G A RV W GYH
Sbjct: 1430KKYSRTVVMQSFLDSHIDPTGWAEWDAASKDFLQTLYYGEYLNNGPGAGTAKRVTWPGYH 1609
Query: 487 VLNN-SQAMDFTVNQFIKGDLWLPSTGVSYNAGL 519
V+N ++A FTV Q I+G++WL +TGV++ GL
Sbjct: 1610VINTAAEASKFTVAQLIQGNVWLKNTGVAFTEGL 1711
>TC76732 homologue to PIR|T06468|T06468 pectinesterase (EC 3.1.1.11)
precursor - garden pea, complete
Length = 1860
Score = 392 bits (1006), Expect = e-109
Identities = 207/453 (45%), Positives = 290/453 (63%), Gaps = 8/453 (1%)
Frame = +2
Query: 75 NDNRLSNAISDCLELFDMAYENLKWSI-SATQNHQGNKNNGTGNLSSDLRTWISAVLSYQ 133
N R A++DC EL D++ + + S+ + T+N+ +++ D TW+S+VL+
Sbjct: 374 NSPREEIALNDCEELMDLSMDRVWDSVLTLTKNNIDSQH--------DAHTWLSSVLTNH 529
Query: 134 ETCIDGFEGTNSNVKDHVSGGLDQVISLVKNEL--LLQVVPDSDPSNISN---SGEFPSW 188
TC+DG EG++ V + L +IS ++ L L+ V+P + +G+FPSW
Sbjct: 530 ATCLDGLEGSSRVVME---SDLHDLISRARSSLAVLVSVLPPKANDGFIDEKLNGDFPSW 700
Query: 189 VKAEDEKLLQQDAPPPPADVVVAADGSGNYTTVMDAVKDAPEYNMTRYVIHVKKGIYKEY 248
V ++D +LL+ A+VVVA DGSG + TV AV AP+ TRYVI+VKKG YKE
Sbjct: 701 VTSKDRRLLESSVGDIKANVVVAQDGSGKFKTVAQAVASAPDNGKTRYVIYVKKGTYKEN 880
Query: 249 VEIKKKKWNIMIVGDGMDVTTISGNRSYGHDNITTFRTATFAVSAIGFIARDITFENTAG 308
+EI KKK N+M+VGDGMD T I+G+ ++ D TTF++AT A GFIA+DI F+NTAG
Sbjct: 881 IEIGKKKTNVMLVGDGMDATIITGSLNF-IDGTTTFKSATVAAVGDGFIAQDIRFQNTAG 1057
Query: 309 PENKQAVALRSDSDLSVFYRCGIKGYQDSLYAHSQRQFYKECRIRGTVDFICGNAAAVFQ 368
P+ QAVALR +D SV RC I +QD+LYAHS RQFY++ I GTVDFI GNAA VFQ
Sbjct: 1058PQKHQAVALRVGADQSVINRCKIDAFQDTLYAHSNRQFYRDSYITGTVDFIFGNAAVVFQ 1237
Query: 369 NCLIQPKKPLKDQKNTIAAQSRTHPNQTSGFSFHNCTIIPDPDFQPSNGSIQTFLGRPWK 428
+ +KP+ +QKN + AQ R PNQ + S C +IP D +P GSI+T+LGRPWK
Sbjct: 1238KSKLAARKPMANQKNMVTAQGREDPNQNTATSIQQCDVIPSSDLKPVQGSIKTYLGRPWK 1417
Query: 429 NFSRTVFMESFMSEMIHPEGWLEWE-TNTTYDDTLFFGEYQNKGPGSGVANRVKWRGYHV 487
+SRTV ++S + I P GW EW+ + + TL++GEY N G G+G RV W GYH+
Sbjct: 1418KYSRTVVLQSVVDGHIDPAGWAEWDAASKDFLQTLYYGEYMNSGAGAGTGKRVTWPGYHI 1597
Query: 488 LNN-SQAMDFTVNQFIKGDLWLPSTGVSYNAGL 519
+ N ++A FTV Q I+G++WL +TGV++ GL
Sbjct: 1598IKNAAEASKFTVTQLIQGNVWLKNTGVAFIEGL 1696
>TC85623 similar to GP|13605696|gb|AAK32841.1 At2g45220/F4L23.27 {Arabidopsis
thaliana}, partial (79%)
Length = 2442
Score = 374 bits (959), Expect = e-104
Identities = 212/441 (48%), Positives = 271/441 (61%), Gaps = 3/441 (0%)
Frame = +3
Query: 82 AISDCLELFDMAYENLKWSISATQNHQGNKNNGTGNLSSDLRTWISAVLSYQETCIDGFE 141
A +DCLEL+D + L ++ T + +TW+S L+ ETC +GF
Sbjct: 849 AWADCLELYDFTVQKL------------SQTKYTKCTQYESQTWLSTALTNLETCKNGFY 992
Query: 142 --GTNSNVKDHVSGGLDQVISLVKNELLLQVVPDSDPSNISNSGEFPSWVKAEDEKLLQQ 199
G + V +S + V L+ N L L VP PS FP+WVK D KLLQ
Sbjct: 993 DLGVTNYVLPLLS---NNVTKLLSNTLSLNKVPYQQPSYKDG---FPTWVKPGDRKLLQT 1154
Query: 200 DAPPPPADVVVAADGSGNYTTVMDAVKDAPEYNMTRYVIHVKKGIYKEYVEIKKKKWNIM 259
+ A+VVVA DGSG YTTV A AP RYVI+VK G+Y E VEIK K N+M
Sbjct: 1155 SSAASKANVVVAKDGSGKYTTVKAATDAAPS-GSGRYVIYVKAGVYNEQVEIKAK--NVM 1325
Query: 260 IVGDGMDVTTISGNRSYGHDNITTFRTATFAVSAIGFIARDITFENTAGPENKQAVALRS 319
+VGDG+ T I+G++S G TTFR+AT A + GFIA+DITF NTAG N QAVA RS
Sbjct: 1326 LVGDGIGKTIITGSKSVG-GGTTTFRSATVAATGDGFIAQDITFRNTAGAANHQAVAFRS 1502
Query: 320 DSDLSVFYRCGIKGYQDSLYAHSQRQFYKECRIRGTVDFICGNAAAVFQNCLIQPKKPLK 379
SDLSVFY+C +GYQD+LY HS+RQFY+EC I GTVDFI GNAA V QNC I + P
Sbjct: 1503 GSDLSVFYKCSFEGYQDTLYVHSERQFYRECNIYGTVDFIFGNAAVVLQNCNIFARNP-P 1679
Query: 380 DQKNTIAAQSRTHPNQTSGFSFHNCTIIPDPDFQPSNGSIQTFLGRPWKNFSRTVFMESF 439
+ T+ AQ RT PNQ +G HN + D PS S++++LGRPW+ +SRTVFM++
Sbjct: 1680 AKTITVTAQGRTDPNQNTGIIIHNSRVSAQSDLNPS--SVKSYLGRPWQKYSRTVFMKTV 1853
Query: 440 MSEMIHPEGWLEWETNTTYDDTLFFGEYQNKGPGSGVANRVKWRGYHVLNN-SQAMDFTV 498
+ I+P GWL W+ N DTL++ EY N G GS +NRV W+GYHVL + SQA FTV
Sbjct: 1854 LDGFINPAGWLPWDGNFAL-DTLYYAEYANTGSGSSTSNRVTWKGYHVLTSASQASPFTV 2030
Query: 499 NQFIKGDLWLPSTGVSYNAGL 519
FI G+ W+ +TGV + +GL
Sbjct: 2031 GNFIAGNSWIGNTGVPFTSGL 2093
>TC87503 similar to SP|Q43111|PME3_PHAVU Pectinesterase 3 precursor (EC
3.1.1.11) (Pectin methylesterase 3) (PE 3)., partial
(93%)
Length = 2088
Score = 353 bits (906), Expect = 9e-98
Identities = 191/452 (42%), Positives = 264/452 (58%), Gaps = 12/452 (2%)
Frame = +2
Query: 78 RLSNAISDCLELFDMAYENLKWSISATQNHQGNKNNGTGNLSSDLRTWISAVLSYQETCI 137
R+ AI C + + + L S+S + G K + D+ TW+SA L+ +TC+
Sbjct: 464 RVKKAIGVCDNVLADSLDRLNDSMSTIVD--GGKMLSPAKIR-DVETWLSAALTDHDTCL 634
Query: 138 DGFEGTNSNVKDHVSGGLDQVI---------SLVKNELLLQVVPDSDPSNISNS--GEFP 186
D NS V +++++ SL +++++ + + SN GEFP
Sbjct: 635 DAVGEVNSTAARGVIPEIERIMRNSTEFASNSLAIVSKVIRLLSNFEVSNHHRRLLGEFP 814
Query: 187 SWVKAEDEKLLQQDAPPPPADVVVAADGSGNYTTVMDAVKDAPEYNMTRYVIHVKKGIYK 246
W+ + +LL D VVA DGSG Y T+ +A+K + ++ R+V++VKKG+Y
Sbjct: 815 EWLGTAERRLLATVVNETVPDAVVAKDGSGQYKTIGEALKLVKKKSLQRFVVYVKKGVYV 994
Query: 247 EYVEIKKKKWNIMIVGDGMDVTTISGNRSYGHDNITTFRTATFAVSAIGFIARDITFENT 306
E +++ K WN+MI GDGM T +SG+R+Y D TF TATFAV GFIA+DI F NT
Sbjct: 995 ENIDLDKNTWNVMIYGDGMTETVVSGSRNY-IDGTPTFETATFAVKGKGFIAKDIQFLNT 1171
Query: 307 AGPENKQAVALRSDSDLSVFYRCGIKGYQDSLYAHSQRQFYKECRIRGTVDFICGNAAAV 366
AG QAVA+RS SD SVFYRC GYQD+LYAHS RQFY++C I GT+DFI GNAAAV
Sbjct: 1172AGASKHQAVAMRSGSDQSVFYRCSFVGYQDTLYAHSNRQFYRDCDITGTIDFIFGNAAAV 1351
Query: 367 FQNCLIQPKKPLKDQKNTIAAQSRTHPNQTSGFSFHNCTIIPDPDFQPSNGSIQTFLGRP 426
FQNC I P++P+ +Q NTI AQ + PNQ SG T P N T+LGRP
Sbjct: 1352FQNCKIMPRQPMSNQFNTITAQGKKDPNQNSGIVIQKSTFTTLPG---DNLIAPTYLGRP 1522
Query: 427 WKNFSRTVFMESFMSEMIHPEGWLEWETNTTYDDTLFFGEYQNKGPGSGVANRVKWRGYH 486
WK+FS T+ M+S + + P GW+ W N ++ + EYQN GPG+ V RVKW GY
Sbjct: 1523WKDFSTTIIMKSEIGSFLKPVGWISWVANVEPPSSILYAEYQNTGPGADVPGRVKWAGYK 1702
Query: 487 -VLNNSQAMDFTVNQFIKGDLWLPSTGVSYNA 517
L + A+ FTV+ FI+G WLPS V +++
Sbjct: 1703PALGDEDAIKFTVDSFIQGPEWLPSASVQFDS 1798
>TC86464 similar to GP|2098711|gb|AAB57670.1| pectinesterase {Citrus
sinensis}, partial (85%)
Length = 1993
Score = 350 bits (898), Expect = 8e-97
Identities = 212/528 (40%), Positives = 290/528 (54%), Gaps = 35/528 (6%)
Frame = +1
Query: 27 SASTITEFSGLSCLRVSPNRFIDSANKVINLLQNVSYNLSPFTKVNQDN----------- 75
SA T T + L +S I +K+ N +S +L+ T+ + N
Sbjct: 256 SACTTTLYPELCFSAISSEPNI--THKITNHKDVISLSLNITTRAVEHNYFTVEKLLLRK 429
Query: 76 --DNRLSNAISDCLELFDMAYENLKWSISATQNHQGNKNNGTGNLSSDLRTWISAVLSYQ 133
R A+ DCLE D + LK + + + K + DL+T IS+ ++ Q
Sbjct: 430 SLTKREKIALHDCLETIDETLDELKEAQNDLVLYPSKKT--LYQHADDLKTLISSAITNQ 603
Query: 134 ETCIDGF--EGTNSNVKDHVSGGLDQVISLVKNELLL------QVVPDSDPSNI------ 179
TC+DGF + + V+ + G V + N L + + + + + +N+
Sbjct: 604 VTCLDGFSHDDADKEVRKVLQEGQIHVEHMCSNALAMTKNMTDKDIAEFEQTNMVLGSNK 783
Query: 180 -------SNSGEFPSWVKAEDEKLLQQDAPPPPADVVVAADGSGNYTTVMDAVKDAPEYN 232
N +P W+ A D +LLQ ADVVVAADGSGN+ TV +AV AP +
Sbjct: 784 NRKLLEEENGVGWPEWISAGDRRLLQGSTVK--ADVVVAADGSGNFKTVSEAVAAAPLKS 957
Query: 233 MTRYVIHVKKGIYKEYVEIKKKKWNIMIVGDGMDVTTISGNRSYGHDNITTFRTATFAVS 292
RYVI +K G+YKE VE+ KKK NIM +GDG T I+G+R+ D TTF +AT A+
Sbjct: 958 SKRYVIKIKAGVYKENVEVPKKKTNIMFLGDGRTNTIITGSRNVV-DGSTTFHSATVAIV 1134
Query: 293 AIGFIARDITFENTAGPENKQAVALRSDSDLSVFYRCGIKGYQDSLYAHSQRQFYKECRI 352
F+ARDITF+NTAGP QAVALR +DLS FY C I YQD+LY H+ RQF+ C I
Sbjct: 1135GGNFLARDITFQNTAGPAKHQAVALRVGADLSAFYNCDIIAYQDTLYVHNNRQFFVNCFI 1314
Query: 353 RGTVDFICGNAAAVFQNCLIQPKKPLKDQKNTIAAQSRTHPNQTSGFSFHNCTIIPDPDF 412
GTVDFI GN+A VFQNC I ++P QKN + AQ R PNQ +G C I D
Sbjct: 1315SGTVDFIFGNSAVVFQNCDIHARRPNSGQKNMVTAQGRVDPNQNTGIVIQKCRIGATKDL 1494
Query: 413 QPSNGSIQTFLGRPWKNFSRTVFMESFMSEMIHPEGWLEWETNTTYDDTLFFGEYQNKGP 472
+ G+ T+LGRPWK +SRTVFM+S +S++I P GW EW N +TL + EYQN GP
Sbjct: 1495EGVKGNFPTYLGRPWKEYSRTVFMQSSISDVIDPVGWHEWNGNFAL-NTLVYREYQNTGP 1671
Query: 473 GSGVANRVKWRGYHVLNN-SQAMDFTVNQFIKGDLWLPSTGVSYNAGL 519
G+G + RV W+G+ V+ + ++A T FI G WL STG ++ GL
Sbjct: 1672GAGTSKRVTWKGFKVITSAAEAQSSTPGNFIGGSSWLGSTGFPFSLGL 1815
>TC86403 similar to PIR|B86158|B86158 hypothetical protein AAF02886.1
[imported] - Arabidopsis thaliana, partial (63%)
Length = 1929
Score = 343 bits (880), Expect = 9e-95
Identities = 197/474 (41%), Positives = 276/474 (57%), Gaps = 35/474 (7%)
Frame = +3
Query: 82 AISDCLELFDMAYENLKWSISATQNHQGNKNNGTGNLSS----DLRTWISAVLSYQETCI 137
A+ DC L D+ ++ L S N T L S +++T +SA+L+ Q+TC+
Sbjct: 315 ALQDCQSLSDLNFDFLSSSFQTV-------NKTTKFLPSLQGENIQTLLSAILTNQQTCL 473
Query: 138 DGFEGTNS--NVKDHVSGGLDQVISLVKNELLL----QVVPDSDPSNISNS--------- 182
DG + T+S + ++ ++ L L L V P ++ ++ NS
Sbjct: 474 DGLKDTSSAWSFRNGLTIPLSNDTKLYSVSLAFFTKGWVNPKTNKTSFPNSKHSNKGFKN 653
Query: 183 GEFPSWVKAEDE---------KLLQQDAPPPPA---DVV-VAADGSGNYTTVMDAVKDAP 229
G P + ++ KLLQ + D+V V+ DGSGN+TT+ DA+ AP
Sbjct: 654 GRLPLKMTSKTRAIYESVSRRKLLQSNQVGEDVVVRDIVTVSQDGSGNFTTINDAIAAAP 833
Query: 230 EYNMTR---YVIHVKKGIYKEYVEIKKKKWNIMIVGDGMDVTTISGNRSYGHDNITTFRT 286
+++ ++I+V G+Y+EY+ I KKK +M++GDG++ T I+GN S D TTF +
Sbjct: 834 NKSVSSDGYFLIYVTAGVYEEYITIDKKKTYLMMIGDGINKTIITGNHSVV-DGWTTFGS 1010
Query: 287 ATFAVSAIGFIARDITFENTAGPENKQAVALRSDSDLSVFYRCGIKGYQDSLYAHSQRQF 346
TFAV GF+ ++T NTAG QAVALR+ +DLS FY C +GYQD+LY HS RQF
Sbjct: 1011PTFAVVGQGFVGVNMTIRNTAGAVKHQAVALRNGADLSTFYSCSFEGYQDTLYTHSLRQF 1190
Query: 347 YKECRIRGTVDFICGNAAAVFQNCLIQPKKPLKDQKNTIAAQSRTHPNQTSGFSFHNCTI 406
Y+EC I GTVDFI GNA VFQNC + P+ P+ Q N I AQ RT PNQ +G S HNCTI
Sbjct: 1191YRECDIYGTVDFIFGNAKVVFQNCNLYPRLPMSGQFNAITAQGRTDPNQDTGTSIHNCTI 1370
Query: 407 IPDPDFQPSNGSIQTFLGRPWKNFSRTVFMESFMSEMIHPEGWLEWETNTTYDDTLFFGE 466
D SNG++ T+LGRPWK +SRTV+M++FM +I+ GW W+ TL++ E
Sbjct: 1371KATDDLAASNGAVSTYLGRPWKEYSRTVYMQTFMDNVINVAGWRAWDGEFAL-STLYYAE 1547
Query: 467 YQNKGPGSGVANRVKWRGYHVLNNSQAMDFTVNQFIKGDLWLPSTGVSYNAGLL 520
+ N GPGS RV W+GYHV+N + A +FTV F+ GD WLP TGVSY L+
Sbjct: 1548FNNSGPGSSTDGRVTWQGYHVINATDAANFTVANFLLGDDWLPQTGVSYTNSLI 1709
>TC77735 similar to GP|14334646|gb|AAK59501.1 putative pectinesterase
{Arabidopsis thaliana}, partial (59%)
Length = 1963
Score = 343 bits (879), Expect = 1e-94
Identities = 214/573 (37%), Positives = 307/573 (53%), Gaps = 64/573 (11%)
Frame = +2
Query: 12 ILLLIMSLQPCTTP-----SSASTITEF------SGLSCLRVSPN------RFIDSAN-K 53
+LLL S P +P SSA+ T S LS +R SP+ +F N K
Sbjct: 86 LLLLAQSPPPSPSPPPPSSSSAACKTTLYPKLCRSMLSAIRSSPSDPYNYGKFSIKQNLK 265
Query: 54 VINLLQNVSYNLSPFTKVNQDNDNRLSNAISDCLELFDMAYENLKWSISATQNHQGNKNN 113
V L+ V + + + ++ A+ DC +L + + L+ ++ + ++
Sbjct: 266 VARKLEKVFIDFLNRHQSSSSLNHEEVGALVDCKDLNSLNVDYLESISDELKSASSSSSS 445
Query: 114 GTGNLSSDLRTWISAVLSYQETCIDGFEGTNSNVKD------------------------ 149
L + +++SAV + TC DG T SN+ +
Sbjct: 446 SDTELVDKIESYLSAVATNHYTCYDGLVVTKSNIANALAVPLKDATQFYSVSLGLVTEAL 625
Query: 150 -------------------HVSGGLDQVISLVKNELLLQVVPDSDPSNISNSGEFPSWVK 190
V L+++I L++ + Q SN +++ +
Sbjct: 626 SKNMKRNKTRKHGLPNKSFKVRQPLEKLIKLLRTKYSCQ----KTSSNCTSTRTERILKE 793
Query: 191 AEDEKLLQQDAPPPPADVVVAADGSGNYTTVMDAVKDAPEYNMTR---YVIHVKKGIYKE 247
+E +L D V+V+ G N+T++ DA+ AP Y+I+V++G Y+E
Sbjct: 794 SESHGILLNDF------VLVSPYGIANHTSIGDAIAAAPNNTKPEDGYYLIYVREGYYEE 955
Query: 248 YVEIKKKKWNIMIVGDGMDVTTISGNRSYGHDNITTFRTATFAVSAIGFIARDITFENTA 307
YV + K K NI++VGDG++ T I+GN S D TTF ++TFAVS FIA DITF NTA
Sbjct: 956 YVIVPKHKNNILLVGDGINNTIITGNHSV-IDGWTTFNSSTFAVSGERFIAVDITFRNTA 1132
Query: 308 GPENKQAVALRSDSDLSVFYRCGIKGYQDSLYAHSQRQFYKECRIRGTVDFICGNAAAVF 367
GPE QAVA+R+++DLS FYRC +GYQD+LY HS RQFY++C+I GTVDFI GNAA VF
Sbjct: 1133GPEKHQAVAVRNNADLSTFYRCSFEGYQDTLYVHSVRQFYRDCKIYGTVDFIFGNAAVVF 1312
Query: 368 QNCLIQPKKPLKDQKNTIAAQSRTHPNQTSGFSFHNCTIIPDPDFQPSNGSIQTFLGRPW 427
QNC I +KPL +QKN + AQ RT PNQ +G S NCTI D S ++LGRPW
Sbjct: 1313QNCNIYARKPLPNQKNAVTAQGRTDPNQNTGISIQNCTIDAAQDLANDLNSTMSYLGRPW 1492
Query: 428 KNFSRTVFMESFMSEMIHPEGWLEWETNTTYDDTLFFGEYQNKGPGSGVANRVKWRGYHV 487
K +SRTV+M+S++ + + P GWLEW T DT+F+GE+ N GPGS NRV+W G+ +
Sbjct: 1493KIYSRTVYMQSYIGDFVQPSGWLEW-NGTVGLDTIFYGEFNNYGPGSVTNNRVQWPGHFL 1669
Query: 488 LNNSQAMDFTVNQFIKGDLWLPSTGVSYNAGLL 520
LN++QA +FTV F G+ WLP T + Y GLL
Sbjct: 1670LNDTQAWNFTVLNFTLGNTWLPDTDIPYTEGLL 1768
>TC77013 similar to GP|7025485|gb|AAF35897.1| pectin methylesterase isoform
alpha {Vigna radiata}, partial (94%)
Length = 1655
Score = 329 bits (843), Expect = 2e-90
Identities = 163/319 (51%), Positives = 213/319 (66%), Gaps = 1/319 (0%)
Frame = +2
Query: 188 WVKAEDEKLLQQDAPPPPADVVVAADGSGNYTTVMDAVKDAPEYNMTRYVIHVKKGIYKE 247
WV +D KLLQ D+VVA DG+GN+ T+ +A+ AP + TR+VIH+K G Y E
Sbjct: 41 WVSPKDRKLLQAAVNQTKFDLVVAKDGTGNFATIGEAIAAAPNSSATRFVIHIKAGAYFE 220
Query: 248 YVEIKKKKWNIMIVGDGMDVTTISGNRSYGHDNITTFRTATFAVSAIGFIARDITFENTA 307
VE+ KKK N+M+VGDG+ T + +R+ D TTF+++TFAV FIA+ ITFEN+A
Sbjct: 221 NVEVIKKKTNLMLVGDGIGQTVVKASRNVV-DGWTTFQSSTFAVVGDKFIAKGITFENSA 397
Query: 308 GPENKQAVALRSDSDLSVFYRCGIKGYQDSLYAHSQRQFYKECRIRGTVDFICGNAAAVF 367
GP QAVA+R+ +D S FY+C YQD+LY HS RQFY+EC + GTVDFI GNAA VF
Sbjct: 398 GPSKHQAVAVRNGADFSAFYQCSFVAYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAVVF 577
Query: 368 QNCLIQPKKPLKDQKNTIAAQSRTHPNQTSGFSFHNCTIIPDPDFQPSNGSIQTFLGRPW 427
QNC + +KP QKN AQ R PNQ +G S NC I D P + +++LGRPW
Sbjct: 578 QNCNLYARKPDPKQKNLFTAQGREDPNQNTGISILNCKIAAAADLIPVQSTFKSYLGRPW 757
Query: 428 KNFSRTVFMESFMSEMIHPEGWLEWETNTTYDDTLFFGEYQNKGPGSGVANRVKWRGYHV 487
K +SRTV++ SF+ +I P GWLEW T DTLF+GEY+N+GPGS + RV W GY V
Sbjct: 758 KKYSRTVYLNSFIDNLIDPPGWLEW-NGTFALDTLFYGEYKNRGPGSNTSARVTWPGYKV 934
Query: 488 LNN-SQAMDFTVNQFIKGD 505
+ N ++A FTV QFI+G+
Sbjct: 935 ITNATEASQFTVRQFIQGE 991
>NP212791 NP212791|AJ249611.1|CAB65291.1 pectin methyl-esterase PEF1
Length = 1698
Score = 280 bits (717), Expect = 7e-76
Identities = 161/461 (34%), Positives = 248/461 (52%), Gaps = 18/461 (3%)
Frame = +1
Query: 76 DNRLSNAISDCLELFDMAYENLKWSISATQNHQGNKNNGTGNLSSDLRTWISAVLSYQET 135
D+ A+ C E+ D A + + S+ +K + D++ W++ LS+Q+T
Sbjct: 334 DSMTKQAMEICNEVLDYAVDGIHKSVGTLDQFDFHK---LSEYAFDIKVWLTGTLSHQQT 504
Query: 136 CIDGFEGTNSNVKDHVSGGLDQVISLVKN-----ELLLQVVPDSDPSN-------ISNSG 183
C+DGF T ++ + ++ L + L N +++ +++ PS +S+ G
Sbjct: 505 CLDGFVNTKTHAGETMAKVLKTSMELSSNAIDMMDVVSRILKGFHPSQYGVSRRLLSDDG 684
Query: 184 EFPSWVKAEDEKLLQQDAPPPPADVVVAADGSGNYTTVMDAVKDAPEYNMTRYVIHVKKG 243
PSWV LL A+ VVA DGSG + T+ DA+K P N +VI+VK G
Sbjct: 685 -IPSWVSDGHRHLLA--GGNVKANAVVAQDGSGQFKTLTDALKTVPPTNAAPFVIYVKAG 855
Query: 244 IYKEYVEIKKKKWNIMIVGDGMDVTTISGNRSYGHDNITTFRTATFAVSAIGFIARDITF 303
+YKE V + K+ + ++GDG T +G+ +Y D I T++TATF V+ F+A+DI F
Sbjct: 856 VYKETVNVAKEMNYVTVIGDGPTKTKFTGSLNYA-DGINTYKTATFGVNGANFMAKDIGF 1032
Query: 304 ENTAGPENKQAVALRSDSDLSVFYRCGIKGYQDSLYAHSQRQFYKECRIRGTVDFICGNA 363
ENTAG QAVALR +D ++F+ C + G+QD+L+ SQRQFY++C I GT+DF+ G+A
Sbjct: 1033ENTAGTSKFQAVALRVTADQAIFHNCQMDGFQDTLFVESQRQFYRDCAISGTIDFVFGDA 1212
Query: 364 AAVFQNCLIQPKKPLKDQKNTIAAQSRTHPNQTSGFSFHNCTIIPDPDFQPSNGSIQTFL 423
VFQNC + + P K QK + A R N S F + +P + ++L
Sbjct: 1213FGVFQNCKLICRVPAKGQKCLVTAGGRDKQNSASALVFLSSHFTGEPALTSVTPKL-SYL 1389
Query: 424 GRPWKNFSRTVFMESFMSEMIHPEGWLEWETNTTYDDTLFFGEYQNKGPGSGVANRVKWR 483
GRPWK +S+ V M+S + M PEG++ + DT F EY NKGPG+ RVKW
Sbjct: 1390GRPWKLYSKVVIMDSTIDAMFAPEGYMPM-VGGAFKDTCTFYEYNNKGPGADTNLRVKWH 1566
Query: 484 GYHVLNNSQAMDFTVNQFIK------GDLWLPSTGVSYNAG 518
G VL ++ A ++ +F + D W+ +GV Y+ G
Sbjct: 1567GVKVLTSNVAAEYYPGKFFEIVNATARDTWIVKSGVPYSLG 1689
>TC89457 weakly similar to SP|Q43062|PME_PRUPE Pectinesterase PPE8B
precursor (EC 3.1.1.11) (Pectin methylesterase) (PE).
[Peach] {Prunus persica}, partial (34%)
Length = 1415
Score = 253 bits (646), Expect = 1e-67
Identities = 151/286 (52%), Positives = 192/286 (66%)
Frame = +1
Query: 4 SSTLTPTLILLLIMSLQPCTTPSSASTITEFSGLSCLRVSPNRFIDSANKVINLLQNVSY 63
++TL+ T + + L + AS+ +F G S LRVS +F +SA +VI +LQ V
Sbjct: 100 TTTLSITTFIFFFLLLLSLPHSTFASSPNDFVG-SSLRVSTAKFANSAEEVITVLQKVIS 276
Query: 64 NLSPFTKVNQDNDNRLSNAISDCLELFDMAYENLKWSISATQNHQGNKNNGTGNLSSDLR 123
LS FT V +R SNA+SDCL+L DM+ + L SISA Q + K+N TG L+ DLR
Sbjct: 277 ILSRFTNVF--GHSRTSNAVSDCLDLLDMSLDQLNQSISAAQKPK-EKDNSTGKLNCDLR 447
Query: 124 TWISAVLSYQETCIDGFEGTNSNVKDHVSGGLDQVISLVKNELLLQVVPDSDPSNISNSG 183
TW+SAVL Y +TCI+G EG S VK +S GLD V+SLV N LL +VV +D +N
Sbjct: 448 TWLSAVLVYPDTCIEGLEG--SIVKGLISSGLDHVMSLVAN-LLGEVVSGNDDQLATNKD 618
Query: 184 EFPSWVKAEDEKLLQQDAPPPPADVVVAADGSGNYTTVMDAVKDAPEYNMTRYVIHVKKG 243
FPSW++ ED KLLQ + AD VVAADGSG+Y VMDAV APE +M RYVI+VKKG
Sbjct: 619 RFPSWIRDEDTKLLQANGVT--ADAVVAADGSGDYAKVMDAVSAAPESSMKRYVIYVKKG 792
Query: 244 IYKEYVEIKKKKWNIMIVGDGMDVTTISGNRSYGHDNITTFRTATF 289
+Y E VEIKKKKWNIM++G+GMD T ISG+R+Y D TTFR+ATF
Sbjct: 793 VYVENVEIKKKKWNIMLIGEGMDATIISGSRNY-VDGSTTFRSATF 927
>TC91752 similar to GP|6630559|gb|AAF19578.1| putative pectinesterase
{Arabidopsis thaliana}, partial (34%)
Length = 691
Score = 242 bits (617), Expect = 3e-64
Identities = 120/221 (54%), Positives = 158/221 (71%), Gaps = 1/221 (0%)
Frame = +3
Query: 178 NISNSGEFPSWVKAEDEKLLQQDAP-PPPADVVVAADGSGNYTTVMDAVKDAPEYNMTRY 236
++S+ E P+W+ +D KLLQ+D+ AD+VVA DGSG Y T+ A+K P + R
Sbjct: 30 SLSHQNEEPNWLHHKDRKLLQKDSDLKKKADIVVAKDGSGKYKTISAALKHVPNKSDKRT 209
Query: 237 VIHVKKGIYKEYVEIKKKKWNIMIVGDGMDVTTISGNRSYGHDNITTFRTATFAVSAIGF 296
VI+VKKGIY E V ++K KWN+MI+GDGM+VT +SG+ ++ D TF TATFAV F
Sbjct: 210 VIYVKKGIYYENVRVEKTKWNVMIIGDGMNVTIVSGSLNF-IDGTPTFSTATFAVFGRNF 386
Query: 297 IARDITFENTAGPENKQAVALRSDSDLSVFYRCGIKGYQDSLYAHSQRQFYKECRIRGTV 356
IARDI F+NTAGP+ QAVAL + +D +VFY+C + YQD+LYAHS RQFY+EC I GTV
Sbjct: 387 IARDIGFKNTAGPQKHQAVALMTSADQAVFYKCSMDAYQDTLYAHSNRQFYRECNIYGTV 566
Query: 357 DFICGNAAAVFQNCLIQPKKPLKDQKNTIAAQSRTHPNQTS 397
DFI GN+A V QNC I P++P+ Q+NTI AQ +T PN +
Sbjct: 567 DFIFGNSAVVLQNCNILPRQPMPGQQNTITAQGKTDPNMNT 689
>BE319906 similar to SP|Q43062|PME_ Pectinesterase PPE8B precursor (EC
3.1.1.11) (Pectin methylesterase) (PE). [Peach] {Prunus
persica}, partial (36%)
Length = 589
Score = 239 bits (610), Expect = 2e-63
Identities = 124/193 (64%), Positives = 142/193 (73%)
Frame = +2
Query: 214 GSGNYTTVMDAVKDAPEYNMTRYVIHVKKGIYKEYVEIKKKKWNIMIVGDGMDVTTISGN 273
GSG+Y VMDAV APE +M RYVI+VKKG+Y E VEIKKKKWNIM++G+GMD T ISG+
Sbjct: 2 GSGDYAKVMDAVSAAPESSMKRYVIYVKKGVYVENVEIKKKKWNIMLIGEGMDATIISGS 181
Query: 274 RSYGHDNITTFRTATFAVSAIGFIARDITFENTAGPENKQAVALRSDSDLSVFYRCGIKG 333
R+Y D TTFR+ATFAVS GFIARDI+F+NTAG E QAVALRSDSDLSVFYRCGI G
Sbjct: 182 RNYV-DGSTTFRSATFAVSGRGFIARDISFQNTAGAEKHQAVALRSDSDLSVFYRCGIFG 358
Query: 334 YQDSLYAHSQRQFYKECRIRGTVDFICGNAAAVFQNCLIQPKKPLKDQKNTIAAQSRTHP 393
YQDSLY H+ RQFY+EC+I GTVDFI G+A AVFQNC I K+ K P
Sbjct: 359 YQDSLYTHTMRQFYRECKISGTVDFIFGDATAVFQNCQILAKERDA*TKEHCYCSRTKRP 538
Query: 394 NQTSGFSFHNCTI 406
T+ F C I
Sbjct: 539 EPTNWFLLPFCNI 577
Score = 45.1 bits (105), Expect = 7e-05
Identities = 20/36 (55%), Positives = 23/36 (63%)
Frame = +3
Query: 374 PKKPLKDQKNTIAAQSRTHPNQTSGFSFHNCTIIPD 409
PKK + QKNT+ AQ R PNQ +GFSFH T D
Sbjct: 480 PKKGMPKQKNTVTAQGRKDPNQPTGFSFHFATFTAD 587
>BQ138825 similar to PIR|T49922|T49 pectin methylesterase-like protein -
Arabidopsis thaliana, partial (36%)
Length = 658
Score = 201 bits (512), Expect = 4e-52
Identities = 106/218 (48%), Positives = 145/218 (65%), Gaps = 10/218 (4%)
Frame = +1
Query: 223 DAVKDAPEYNMTR--YVIHVKKGIYKEYVEIKKKKWNIMIVGDGMDVTTISGNRSYGHDN 280
+AV AP+ + R +VI++K+G+Y+E V + KK N++ +GDG+ T I+G+ + G
Sbjct: 4 EAVNAAPDNGVDRKRFVIYIKEGVYEETVRVPLKKRNVVFLGDGIGKTVITGSANVGQPG 183
Query: 281 ITTFRTATFAVSAIGFIARDITFENTAGPENKQAVALRSDSDLSVFYRCGIKGYQDSLYA 340
+TT+ +AT AV GF+A+D+T ENTAGP+ QAVA R DSDLSV C G QD+LYA
Sbjct: 184 MTTYNSATVAVLGDGFMAKDLTIENTAGPDAHQAVAFRLDSDLSVIENCEFLGNQDTLYA 363
Query: 341 HSQRQFYKECRIRGTVDFICGNAAAVFQNC--LIQPK--KPLKDQKNTIAAQSRTHPNQT 396
HS RQFYK CRI G VDFI GN+AA+FQ+C L++P+ KP K + N I A RT P Q+
Sbjct: 364 HSLRQFYKSCRIVGNVDFIFGNSAAIFQDCQILVRPRQLKPEKGENNAITAHGRTDPAQS 543
Query: 397 SGFSFHNCTIIPDPDFQP---SNGSI-QTFLGRPWKNF 430
+GF F NC I D+ SN + + +LGRPWK +
Sbjct: 544 TGFVFQNCLINGTEDYMALYHSNPKVHKNYLGRPWKEY 657
>TC89627 weakly similar to GP|17529290|gb|AAL38872.1 putative pectin
methylesterase {Arabidopsis thaliana}, partial (33%)
Length = 1289
Score = 146 bits (368), Expect(2) = 1e-48
Identities = 84/236 (35%), Positives = 130/236 (54%), Gaps = 9/236 (3%)
Frame = +3
Query: 210 VAADGSGNYTTVMDAVKDAPEYNMTRYVIHVKKGIYKEYVEIKKKKWNIMIVGDGMDVTT 269
V+ DGS + ++ +A+ YN+ R +I + G Y+E + + K I +GD D T
Sbjct: 243 VSQDGSAQFKSITEALNSIQPYNIRRVIISIAPGYYREKIVVPKTLPFITFLGDVRDPPT 422
Query: 270 ISGNRSY---GHDN--ITTFRTATFAVSAIGFIARDITFENTA----GPENKQAVALRSD 320
I+GN + G D + TF +AT AV+A F+A +I FENTA G + +QAVA+R
Sbjct: 423 ITGNDTQSVTGSDGAQLRTFNSATVAVNASYFMAININFENTASFPIGSKVEQAVAVRIT 602
Query: 321 SDLSVFYRCGIKGYQDSLYAHSQRQFYKECRIRGTVDFICGNAAAVFQNCLIQPKKPLKD 380
+ + FY C G QD+LY H ++ C I+G+VDFICG+ ++++ C I + + +
Sbjct: 603 GNKTAFYNCTFSGVQDTLYDHKGLHYFNNCTIKGSVDFICGHGKSLYEGCTI---RSIAN 773
Query: 381 QKNTIAAQSRTHPNQTSGFSFHNCTIIPDPDFQPSNGSIQTFLGRPWKNFSRTVFM 436
+I AQS ++P+ SGFSF N +I G T+LGRPW + F+
Sbjct: 774 NMTSITAQSGSNPSYDSGFSFKNSMVI---------GDGPTYLGRPWGKLFTSCFL 914
Score = 65.5 bits (158), Expect(2) = 1e-48
Identities = 31/82 (37%), Positives = 47/82 (56%)
Frame = +1
Query: 429 NFSRTVFMESFMSEMIHPEGWLEWETNTTYDDTLFFGEYQNKGPGSGVANRVKWRGYHVL 488
N+S+ VF ++M + P+GW +W Y + ++GEY+ GPGS A RV W +L
Sbjct: 892 NYSQVVFSYTYMDNSVLPKGWEDWNDTKRYMNA-YYGEYKCSGPGSNTAGRVPWA--RML 1062
Query: 489 NNSQAMDFTVNQFIKGDLWLPS 510
N+ +A F Q+I G+ WL S
Sbjct: 1063NDKEAQVFIGTQYIDGNTWLIS 1128
>BI272853 weakly similar to PIR|T04359|T04 pectin methylesterase-like protein
- maize, partial (25%)
Length = 678
Score = 179 bits (453), Expect = 3e-45
Identities = 91/204 (44%), Positives = 124/204 (60%)
Frame = +1
Query: 235 RYVIHVKKGIYKEYVEIKKKKWNIMIVGDGMDVTTISGNRSYGHDNITTFRTATFAVSAI 294
RY I+VK G+Y EY+ I K NI++ GDG T ++G ++ G + T +TATFA +A+
Sbjct: 4 RYTIYVKAGVYDEYITIPKDAVNILMYGDGPGKTIVTGRKN-GAAGVKTMQTATFANTAL 180
Query: 295 GFIARDITFENTAGPENKQAVALRSDSDLSVFYRCGIKGYQDSLYAHSQRQFYKECRIRG 354
GFI + +TFENTAGP QAVA R+ D+S C I GYQD+LY + RQFY+ C I G
Sbjct: 181 GFIGKAMTFENTAGPAGHQAVAFRNQGDMSALVGCHILGYQDTLYVQTNRQFYRNCVISG 360
Query: 355 TVDFICGNAAAVFQNCLIQPKKPLKDQKNTIAAQSRTHPNQTSGFSFHNCTIIPDPDFQP 414
TVDFI G +A + Q+ I + P +Q NTI A +G C I+P P
Sbjct: 361 TVDFIFGTSATLIQDSTIIVRMPSPNQFNTITADGSYVNKLNTGIVIQGCNIVPKAALFP 540
Query: 415 SNGSIQTFLGRPWKNFSRTVFMES 438
+I+++LGRPWK ++TV MES
Sbjct: 541 QRFTIKSYLGRPWKVLTKTVVMES 612
>TC84419 similar to SP|Q42920|PME_MEDSA Pectinesterase precursor (EC
3.1.1.11) (Pectin methylesterase) (PE) (P65). [Alfalfa],
partial (53%)
Length = 732
Score = 168 bits (425), Expect = 5e-42
Identities = 80/201 (39%), Positives = 121/201 (59%)
Frame = +2
Query: 296 FIARDITFENTAGPENKQAVALRSDSDLSVFYRCGIKGYQDSLYAHSQRQFYKECRIRGT 355
F A ++ FEN+AG QAVALR +D ++FY C + GYQD+LY S+RQFY++C I GT
Sbjct: 86 FTALNVGFENSAGAAKHQAVALRVTADKALFYNCQMNGYQDTLYTQSKRQFYRDCTITGT 265
Query: 356 VDFICGNAAAVFQNCLIQPKKPLKDQKNTIAAQSRTHPNQTSGFSFHNCTIIPDPDFQPS 415
+DF+ G+A VFQNC + +KP+ +Q+ + A RT + S F NC +P+
Sbjct: 266 IDFVFGDAVGVFQNCKLIVRKPMANQQCMVTAGGRTKVDSVSALVFQNCHFTGEPEVLTM 445
Query: 416 NGSIQTFLGRPWKNFSRTVFMESFMSEMIHPEGWLEWETNTTYDDTLFFGEYQNKGPGSG 475
I +LGRPW+NFS+ V ++S + + PEG++ W N + +T + EY NKG G+
Sbjct: 446 QPKI-AYLGRPWRNFSKVVIVDSLIDGLFVPEGYMPWMGN-LFKETCTYLEYNNKGAGAA 619
Query: 476 VANRVKWRGYHVLNNSQAMDF 496
+VKW G ++ +A +
Sbjct: 620 TNLKVKWPGVKTISAGEAAKY 682
>AL368659 similar to PIR|B86158|B86 hypothetical protein AAF02886.1
[imported] - Arabidopsis thaliana, partial (25%)
Length = 495
Score = 155 bits (393), Expect = 3e-38
Identities = 77/145 (53%), Positives = 103/145 (70%), Gaps = 3/145 (2%)
Frame = +1
Query: 213 DGSGNYTTVMDAVKDAPEYNMTR---YVIHVKKGIYKEYVEIKKKKWNIMIVGDGMDVTT 269
DGSGN+TT+ DA+ AP + ++I + +G+Y+EYV I KKK +M++G+G++ T
Sbjct: 64 DGSGNFTTINDAIAAAPNNTVASDGYFLIFITEGVYEEYVSIDKKKKYLMMIGEGINQTI 243
Query: 270 ISGNRSYGHDNITTFRTATFAVSAIGFIARDITFENTAGPENKQAVALRSDSDLSVFYRC 329
I+GNR+ D TTF +ATFAV A GF+A +ITF NTAG QAVALRS +D+S FY C
Sbjct: 244 ITGNRNVA-DGFTTFNSATFAVVAQGFVAVNITFRNTAGAAKNQAVALRSGADMSTFYSC 420
Query: 330 GIKGYQDSLYAHSQRQFYKECRIRG 354
+GYQD+LY HS RQFY+EC I G
Sbjct: 421 SFEGYQDTLYTHSLRQFYRECDIYG 495
>TC93392 similar to PIR|T49241|T49241 pectinesterase-like protein -
Arabidopsis thaliana, partial (18%)
Length = 467
Score = 140 bits (352), Expect = 2e-33
Identities = 65/101 (64%), Positives = 81/101 (79%), Gaps = 1/101 (0%)
Frame = +2
Query: 422 FLGRPWKNFSRTVFMESFMSEMIHPEGWLEWETNTTYDDTLFFGEYQNKGPGSGVANRVK 481
+LGRPWK +SRT+FM+S+MS+ I PEGWLEW N +TL++ EY N GPG+GVANRVK
Sbjct: 2 YLGRPWKTYSRTIFMQSYMSDAIRPEGWLEWNGNFAL-NTLYYAEYMNSGPGAGVANRVK 178
Query: 482 WRGYHVLN-NSQAMDFTVNQFIKGDLWLPSTGVSYNAGLLV 521
W GYHVLN +S+A FTV QFI+G+L LPSTGV+Y +GL V
Sbjct: 179 WSGYHVLNDSSEATKFTVAQFIEGNLGLPSTGVTYTSGLKV 301
>TC82059 similar to GP|19424045|gb|AAL87311.1 unknown protein {Arabidopsis
thaliana}, partial (17%)
Length = 483
Score = 125 bits (315), Expect = 3e-29
Identities = 53/103 (51%), Positives = 73/103 (70%)
Frame = +1
Query: 418 SIQTFLGRPWKNFSRTVFMESFMSEMIHPEGWLEWETNTTYDDTLFFGEYQNKGPGSGVA 477
+++T+LGRPWK +SRTV+M+SFM I+P GW EW + TL++ EY N+G GS A
Sbjct: 10 TVKTYLGRPWKEYSRTVYMQSFMDSFINPSGWREWNGDFALS-TLYYAEYDNRGAGSSTA 186
Query: 478 NRVKWRGYHVLNNSQAMDFTVNQFIKGDLWLPSTGVSYNAGLL 520
NRV W GYHV+ + A +FTV+ F+ GD W+P TGV Y +GL+
Sbjct: 187 NRVTWPGYHVIGATDAANFTVSNFVSGDDWIPQTGVPYMSGLV 315
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.316 0.133 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,292,859
Number of Sequences: 36976
Number of extensions: 256994
Number of successful extensions: 1444
Number of sequences better than 10.0: 80
Number of HSP's better than 10.0 without gapping: 1348
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1368
length of query: 521
length of database: 9,014,727
effective HSP length: 100
effective length of query: 421
effective length of database: 5,317,127
effective search space: 2238510467
effective search space used: 2238510467
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0343.3