BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0342b.11
(137 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC87330 homologue to SP|P04571|SCP_NERDI Sarcoplasmic calcium-bi... 221 5e-59
TC87331 similar to GP|4261574|gb|AAD13874.1| Unknown {Helianthus... 221 9e-59
TC82334 similar to GP|18252875|gb|AAL62364.1 unknown protein {Ar... 126 2e-30
TC86968 similar to PIR|T07107|T07107 probable calcium-binding pr... 53 4e-08
AW573662 weakly similar to PIR|D96689|D966 calmodulin-related pr... 35 0.012
AL382510 weakly similar to GP|13194672|gb| calmodulin-like prote... 32 0.061
BI272436 weakly similar to PIR|T05057|T050 hypothetical protein ... 32 0.079
BG450067 weakly similar to PIR|T05057|T050 hypothetical protein ... 31 0.14
TC79660 similar to PIR|T05057|T05057 hypothetical protein M3E9.1... 31 0.14
TC88335 weakly similar to GP|3063551|gb|AAC14087.1| unknown {Try... 30 0.30
CB894150 30 0.30
TC87949 similar to GP|16904224|gb|AAL30819.1 calcium/calmodulin-... 30 0.30
TC87227 similar to GP|17064926|gb|AAL32617.1 calcium-dependent p... 30 0.39
TC82134 similar to PIR|T07949|T07949 calcium binding protein - l... 28 1.1
TC77934 similar to GP|9965747|gb|AAG10150.1| calmodulin-like MSS... 28 1.1
TC83765 similar to SP|P87072|CALB_NEUCR Calcineurin B subunit (P... 27 2.6
BM814976 similar to SP|Q43070|GAE1 UDP-glucose 4-epimerase (EC 5... 26 4.4
TC81309 homologue to GP|10177565|dbj|BAB10797. deoxyhypusine syn... 26 5.7
TC93152 26 5.7
TC76424 similar to GP|14335106|gb|AAK59832.1 At2g41900/T6D20.20 ... 26 5.7
>TC87330 homologue to SP|P04571|SCP_NERDI Sarcoplasmic calcium-binding
protein (SCP). [Sandworm Hediste diversicolor] {Nereis
diversicolor}, partial (6%)
Length = 835
Score = 221 bits (564), Expect = 5e-59
Identities = 110/137 (80%), Positives = 123/137 (89%)
Frame = +1
Query: 1 MSVEILDGATIVNFLEDEEAFNISVCNRFALLDTNKDGLLSYEEMLKELQCLRVLETHFG 60
MSVEILDGATIV+FLEDEEAFN SV NRFA LDTN DGLLSYEEMLKEL+ LR+ ETHFG
Sbjct: 292 MSVEILDGATIVHFLEDEEAFNSSVGNRFARLDTNNDGLLSYEEMLKELRSLRIFETHFG 471
Query: 61 IDVKPDPDELGRVYKSLFIQFDHNLNGSVDLEEFKMETREMMLAMADGMGFLPVQMALEE 120
IDV DP+EL RVY+SLFIQFDHNLNG++DLEEFK ET++MMLAMADGMGF+P+QM LEE
Sbjct: 472 IDVDSDPNELARVYESLFIQFDHNLNGTIDLEEFKKETKQMMLAMADGMGFMPIQMVLEE 651
Query: 121 DSILKRAVERECNKVVA 137
DSILK+AVE + NKV A
Sbjct: 652 DSILKKAVEIDSNKVAA 702
>TC87331 similar to GP|4261574|gb|AAD13874.1| Unknown {Helianthus}, partial
(22%)
Length = 1196
Score = 221 bits (562), Expect = 9e-59
Identities = 111/137 (81%), Positives = 124/137 (90%)
Frame = +1
Query: 1 MSVEILDGATIVNFLEDEEAFNISVCNRFALLDTNKDGLLSYEEMLKELQCLRVLETHFG 60
MSVEILDGATIV+FLEDEEAFN SV NRFA LDTNKDGLLSY+EMLKEL+ LRV ETHFG
Sbjct: 85 MSVEILDGATIVHFLEDEEAFNSSVGNRFARLDTNKDGLLSYDEMLKELRSLRVFETHFG 264
Query: 61 IDVKPDPDELGRVYKSLFIQFDHNLNGSVDLEEFKMETREMMLAMADGMGFLPVQMALEE 120
IDV DP+EL RVY+SLFIQFDHNLNG+VDLEEFK ET+++MLAMADGMGF+P+QM LEE
Sbjct: 265 IDVDSDPNELARVYESLFIQFDHNLNGTVDLEEFKKETKQIMLAMADGMGFMPIQMVLEE 444
Query: 121 DSILKRAVERECNKVVA 137
DSILK AVERE +K+ A
Sbjct: 445 DSILKIAVERESSKLAA 495
>TC82334 similar to GP|18252875|gb|AAL62364.1 unknown protein {Arabidopsis
thaliana}, partial (73%)
Length = 585
Score = 126 bits (317), Expect = 2e-30
Identities = 64/140 (45%), Positives = 96/140 (67%), Gaps = 3/140 (2%)
Frame = +2
Query: 1 MSVEILDGATIVNFLEDEEAFNISVCNRFALLDTNKDGLLSYEEMLKELQCLRVLETHFG 60
M V I+DG T+ +F+ D+ F S+ +F LD N DG+LS E+ K + +R++E+HFG
Sbjct: 2 MGVVIIDGTTVRDFIADDTHFTNSINEQFTSLDLNNDGVLSRAELRKAFESMRLIESHFG 181
Query: 61 IDVKPDPDELGRVYKSLFIQFDHNLNGSVDLEEFKMETREMMLAMADGMGFLPVQMALEE 120
IDV P++L R+Y S+F FD + +GSVD EEFK E +++MLA+ADG+G P+QM LE+
Sbjct: 182 IDVTTPPEQLTRLYDSVFDTFDGDGSGSVDREEFKNEMKKIMLAIADGLGSSPIQMVLED 361
Query: 121 D---SILKRAVERECNKVVA 137
D ++LK+A + E +K A
Sbjct: 362 DDEMNLLKKAADLEASKNAA 421
>TC86968 similar to PIR|T07107|T07107 probable calcium-binding protein
(clone Y8) - potato, partial (89%)
Length = 1691
Score = 52.8 bits (125), Expect = 4e-08
Identities = 34/114 (29%), Positives = 59/114 (50%), Gaps = 1/114 (0%)
Frame = +1
Query: 2 SVEILDGATIVNFLEDEEAFNISVCNRFALLDTNKDGLLSYEEMLKELQ-CLRVLETHFG 60
+VE+LDG+ I+ + +++ F+ V ++F LD +KDG LS L ELQ + + + G
Sbjct: 184 AVEVLDGSNIMELVGNKDVFSNFVNHKFQELDRDKDGKLS----LTELQPAVSDIGSALG 351
Query: 61 IDVKPDPDELGRVYKSLFIQFDHNLNGSVDLEEFKMETREMMLAMADGMGFLPV 114
+ K + +Y + +F H V EFK +++L MA G+ P+
Sbjct: 352 LPAKGTNPDSDHIYSEVLNEFTHGKQEKVSKTEFKEVLSDILLGMAAGLKRDPI 513
>AW573662 weakly similar to PIR|D96689|D966 calmodulin-related protein
72976-72503 [imported] - Arabidopsis thaliana, partial
(29%)
Length = 615
Score = 34.7 bits (78), Expect = 0.012
Identities = 25/91 (27%), Positives = 43/91 (46%)
Frame = +1
Query: 10 TIVNFLEDEEAFNISVCNRFALLDTNKDGLLSYEEMLKELQCLRVLETHFGIDVKPDPDE 69
+I +F+++EE+ F L+DTN DG +S E+ + L CL +
Sbjct: 76 SISSFIQNEESGQFRQI--FKLIDTNNDGKISTTELSEVLSCLGY-----------NKCT 216
Query: 70 LGRVYKSLFIQFDHNLNGSVDLEEFKMETRE 100
+ +S+ D N +G VD++EF +E
Sbjct: 217 ASKEAESMIRVLDFNGDGFVDIDEFMFVMKE 309
>AL382510 weakly similar to GP|13194672|gb| calmodulin-like protein
{Pennisetum ciliare}, partial (24%)
Length = 399
Score = 32.3 bits (72), Expect = 0.061
Identities = 23/86 (26%), Positives = 40/86 (45%)
Frame = +1
Query: 11 IVNFLEDEEAFNISVCNRFALLDTNKDGLLSYEEMLKELQCLRVLETHFGIDVKPDPDEL 70
++ LED + + F + D + DG + E+ + L CL G+D D + +
Sbjct: 55 VLGELEDMSKKSELLLEAFKIFDEDGDGYIDAMELKRVLDCL-------GLDKGWDMNTI 213
Query: 71 GRVYKSLFIQFDHNLNGSVDLEEFKM 96
R+ K + + FD G VD EF++
Sbjct: 214 ERMVKVVDLNFD----GKVDFGEFEL 279
>BI272436 weakly similar to PIR|T05057|T050 hypothetical protein M3E9.100 -
Arabidopsis thaliana, partial (37%)
Length = 572
Score = 32.0 bits (71), Expect = 0.079
Identities = 15/44 (34%), Positives = 26/44 (59%)
Frame = +2
Query: 65 PDPDELGRVYKSLFIQFDHNLNGSVDLEEFKMETREMMLAMADG 108
P DE R +++F QFD + NG++D EE K ++ + ++G
Sbjct: 392 PKIDESFRKCRAIFEQFDEDSNGTIDQEELKKCFSKLEXSFSEG 523
>BG450067 weakly similar to PIR|T05057|T050 hypothetical protein M3E9.100 -
Arabidopsis thaliana, partial (39%)
Length = 525
Score = 31.2 bits (69), Expect = 0.14
Identities = 14/31 (45%), Positives = 20/31 (64%)
Frame = +1
Query: 65 PDPDELGRVYKSLFIQFDHNLNGSVDLEEFK 95
P DE R +++F QFD + NG++D EE K
Sbjct: 340 PKIDESFRKCRAIFEQFDEDSNGTIDQEELK 432
>TC79660 similar to PIR|T05057|T05057 hypothetical protein M3E9.100 -
Arabidopsis thaliana, partial (85%)
Length = 1161
Score = 31.2 bits (69), Expect = 0.14
Identities = 14/31 (45%), Positives = 20/31 (64%)
Frame = +2
Query: 65 PDPDELGRVYKSLFIQFDHNLNGSVDLEEFK 95
P DE R +++F QFD + NG++D EE K
Sbjct: 266 PKIDESFRKCRAIFEQFDEDSNGTIDQEELK 358
>TC88335 weakly similar to GP|3063551|gb|AAC14087.1| unknown {Trypanosoma
cruzi}, partial (3%)
Length = 970
Score = 30.0 bits (66), Expect = 0.30
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = -2
Query: 3 VEILDGATIVNFLEDEEAFNISVCNRFALLDTNKDGLLSYEEMLKEL 49
+ ILDG+T+ EDE+ F + N F LD G +S ++ L
Sbjct: 498 ISILDGSTLRLLFEDEDDFAMLAENLFTDLDVEDKGKISKSQIRSAL 358
>CB894150
Length = 812
Score = 30.0 bits (66), Expect = 0.30
Identities = 16/47 (34%), Positives = 24/47 (51%)
Frame = +3
Query: 3 VEILDGATIVNFLEDEEAFNISVCNRFALLDTNKDGLLSYEEMLKEL 49
+ ILDG+T+ EDE+ F + N F LD G +S ++ L
Sbjct: 510 ISILDGSTLRLLFEDEDDFAMLAENLFTDLDVEDKGKISKSQIRSAL 650
>TC87949 similar to GP|16904224|gb|AAL30819.1 calcium/calmodulin-dependent
protein kinase CaMK2 {Nicotiana tabacum}, partial (62%)
Length = 1413
Score = 30.0 bits (66), Expect = 0.30
Identities = 18/65 (27%), Positives = 33/65 (50%)
Frame = +1
Query: 32 LDTNKDGLLSYEEMLKELQCLRVLETHFGIDVKPDPDELGRVYKSLFIQFDHNLNGSVDL 91
+D+N DGL+ + E + + LE H D D+ + ++ F +FD + +G +
Sbjct: 901 IDSNTDGLVDFTEFVAATLHVHQLEEH-------DSDKWQQRSQAAFEKFDIDKDGYITP 1059
Query: 92 EEFKM 96
EE +M
Sbjct: 1060EELRM 1074
>TC87227 similar to GP|17064926|gb|AAL32617.1 calcium-dependent protein kinase
{Arabidopsis thaliana}, partial (95%)
Length = 2319
Score = 29.6 bits (65), Expect = 0.39
Identities = 23/85 (27%), Positives = 39/85 (45%), Gaps = 1/85 (1%)
Frame = +1
Query: 11 IVNFLEDEEAFNISVCNRFALLDTNKDGLLSYEEMLKELQCLRVLETHFGIDVKPDPDEL 70
I +FL +EE +I F +DT+ DG++S EE+ + + +L
Sbjct: 1252 IADFLSNEEVEDIK--EMFKKMDTDNDGIVSIEEL--------------KVGFRNHQSQL 1383
Query: 71 GRVYKSLFIQ-FDHNLNGSVDLEEF 94
+FI+ D+N G++D EF
Sbjct: 1384 AESEVQMFIEAVDNNGKGTLDYGEF 1458
>TC82134 similar to PIR|T07949|T07949 calcium binding protein - loblolly
pine, partial (27%)
Length = 630
Score = 28.1 bits (61), Expect = 1.1
Identities = 14/44 (31%), Positives = 24/44 (53%)
Frame = +1
Query: 64 KPDPDELGRVYKSLFIQFDHNLNGSVDLEEFKMETREMMLAMAD 107
+P DE+ K +F +FD N +G + LEE+K + + + D
Sbjct: 52 QPKKDEM----KWVFEKFDTNKDGKISLEEYKAAAKSLDKGIGD 171
Score = 26.6 bits (57), Expect = 3.3
Identities = 21/66 (31%), Positives = 29/66 (43%)
Frame = +1
Query: 29 FALLDTNKDGLLSYEEMLKELQCLRVLETHFGIDVKPDPDELGRVYKSLFIQFDHNLNGS 88
F DTNKDG +S EE + L+ G DPD + F D + +G
Sbjct: 82 FEKFDTNKDGKISLEEY---KAAAKSLDKGIG-----DPDAV-----KAFNVMDSDKDGF 222
Query: 89 VDLEEF 94
+D +EF
Sbjct: 223 IDFKEF 240
>TC77934 similar to GP|9965747|gb|AAG10150.1| calmodulin-like MSS3
{Arabidopsis thaliana}, partial (80%)
Length = 1325
Score = 28.1 bits (61), Expect = 1.1
Identities = 25/79 (31%), Positives = 42/79 (52%)
Frame = +1
Query: 29 FALLDTNKDGLLSYEEMLKELQCLRVLETHFGIDVKPDPDELGRVYKSLFIQFDHNLNGS 88
F + D N DG ++ +E+ L+ L GI + PD EL ++ + + D N +G
Sbjct: 301 FQMFDRNDDGRITKKELNDSLENL-------GIFI-PDK-ELSQMIEKI----DVNRDGC 441
Query: 89 VDLEEFKMETREMMLAMAD 107
VD+EEF+ E E +++ D
Sbjct: 442 VDIEEFR-ELYESIMSERD 495
>TC83765 similar to SP|P87072|CALB_NEUCR Calcineurin B subunit (Protein
phosphatase 2B regulatory subunit) (Calcineurin
regulatory subunit)., partial (89%)
Length = 732
Score = 26.9 bits (58), Expect = 2.6
Identities = 12/27 (44%), Positives = 20/27 (73%)
Frame = +1
Query: 68 DELGRVYKSLFIQFDHNLNGSVDLEEF 94
+E+ R+YK F++ D + +GS+D EEF
Sbjct: 19 EEIQRLYKR-FMKLDKDRSGSIDREEF 96
>BM814976 similar to SP|Q43070|GAE1 UDP-glucose 4-epimerase (EC 5.1.3.2)
(Galactowaldenase) (UDP- galactose 4-epimerase). [Garden
pea], partial (51%)
Length = 576
Score = 26.2 bits (56), Expect = 4.4
Identities = 14/43 (32%), Positives = 26/43 (59%), Gaps = 2/43 (4%)
Frame = +2
Query: 81 FDHNLNGSVDLEEF--KMETREMMLAMADGMGFLPVQMALEED 121
FDHNL GS++L + K + ++M+L+ + + P ++ ED
Sbjct: 152 FDHNLVGSINLFQVMAKFQCKKMVLSSSAAVYGQPEEIPCVED 280
>TC81309 homologue to GP|10177565|dbj|BAB10797. deoxyhypusine synthase
{Arabidopsis thaliana}, partial (56%)
Length = 1225
Score = 25.8 bits (55), Expect = 5.7
Identities = 15/42 (35%), Positives = 20/42 (46%), Gaps = 2/42 (4%)
Frame = +1
Query: 24 SVCNRFALLDTNKDGLLSY--EEMLKELQCLRVLETHFGIDV 63
S C F K G +SY + + CLR+L FGID+
Sbjct: 949 SECQMFWYGT*TKVGCMSYLRSKFIYNAVCLRILMDQFGIDI 1074
>TC93152
Length = 647
Score = 25.8 bits (55), Expect = 5.7
Identities = 29/98 (29%), Positives = 43/98 (43%), Gaps = 12/98 (12%)
Frame = +3
Query: 45 MLKELQCLRV----LETHFGIDVKPDPD-ELGRVYKSLFIQFD-HNLNGSVDLEEFKM-- 96
++K+LQ R L TH+ D+ DPD E G V SL H S ++ K+
Sbjct: 345 LIKDLQSAREAWNELSTHYSSDLSADPDIEQGVVDDSLLKNKSLHMFIESGAWKDAKLFM 524
Query: 97 --ETREMMLAMADGMG--FLPVQMALEEDSILKRAVER 130
+M + MG L V M + I+K+ V+R
Sbjct: 525 TDSNNDMAIFSTSSMGRTVLHVAMIAGREGIVKKLVKR 638
>TC76424 similar to GP|14335106|gb|AAK59832.1 At2g41900/T6D20.20
{Arabidopsis thaliana}, partial (12%)
Length = 1429
Score = 25.8 bits (55), Expect = 5.7
Identities = 14/34 (41%), Positives = 17/34 (49%)
Frame = -1
Query: 49 LQCLRVLETHFGIDVKPDPDELGRVYKSLFIQFD 82
L CLRV +THF +P D+ V IQ D
Sbjct: 892 LPCLRVSKTHFSF-AEPQDDQAASVQAMFPIQHD 794
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.321 0.139 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,390,370
Number of Sequences: 36976
Number of extensions: 37149
Number of successful extensions: 159
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 155
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 158
length of query: 137
length of database: 9,014,727
effective HSP length: 86
effective length of query: 51
effective length of database: 5,834,791
effective search space: 297574341
effective search space used: 297574341
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)
Lotus: description of TM0342b.11