BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0336.2
(88 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC77405 similar to GP|21360368|gb|AAM47506.1 iron-stress related... 122 2e-29
TC77910 similar to GP|23345021|gb|AAN17502.1 omega-3 fatty acid ... 32 0.043
TC89720 similar to GP|1492084|gb|AAC55269.1| MC141R {Molluscum c... 30 0.16
BF642460 similar to GP|20197062|gb| putative beta-galactosidase ... 29 0.36
TC83119 similar to GP|13357253|gb|AAK20050.1 putative zinc finge... 29 0.36
BM815814 similar to GP|12328527|db hypothetical protein~similar ... 28 0.61
BF632420 weakly similar to GP|21618116|gb| unknown {Arabidopsis ... 28 0.80
BE324815 27 1.0
TC87839 similar to GP|20259677|gb|AAM14356.1 unknown protein {Ar... 27 1.0
TC83798 weakly similar to GP|10177750|dbj|BAB11063. cytochrome P... 27 1.0
AL387977 27 1.8
BF633093 similar to PIR|T47568|T475 fructokinase-like protein - ... 27 1.8
BE239772 27 1.8
TC80738 similar to GP|16226347|gb|AAL16142.1 AT3g47850/T23J7_180... 27 1.8
TC78143 homologue to PIR|T47615|T47615 histone H2A.F/Z - Arabido... 27 1.8
CA859527 26 2.3
TC88239 homologue to PIR|G96718|G96718 unknown protein 54453-53... 26 2.3
TC85312 homologue to GP|6164920|gb|AAF04584.1| type 1 metallothi... 26 2.3
BQ137654 homologue to GP|6164920|gb|AA type 1 metallothionein {M... 26 2.3
TC83065 similar to GP|20258842|gb|AAM13903.1 putative cytochrome... 26 2.3
>TC77405 similar to GP|21360368|gb|AAM47506.1 iron-stress related protein
{Citrus junos}, partial (13%)
Length = 944
Score = 122 bits (307), Expect = 2e-29
Identities = 62/88 (70%), Positives = 72/88 (81%)
Frame = +3
Query: 1 SDRLNRTNSIGNDLEKNQVASSRNVQVQDHIDAVSSILVNENHYSRFVALDGCSCSCSCS 60
SDRL+R+NS+G+D+EKNQVASS+N+QV DH A SS + NENHYSRF+AL G CS
Sbjct: 186 SDRLDRSNSVGSDIEKNQVASSKNIQVTDHGHASSSNIFNENHYSRFIALQG------CS 347
Query: 61 CSAASQLLNDRWTAAIQSYNNSKDSSEK 88
SAASQLL+DRWTA IQSYNN KDSSEK
Sbjct: 348 SSAASQLLSDRWTAVIQSYNNPKDSSEK 431
>TC77910 similar to GP|23345021|gb|AAN17502.1 omega-3 fatty acid desaturase
{Betula pendula}, partial (84%)
Length = 1750
Score = 32.0 bits (71), Expect = 0.043
Identities = 12/17 (70%), Positives = 13/17 (75%)
Frame = -1
Query: 51 DGCSCSCSCSCSAASQL 67
+ CSCSCSCSCS S L
Sbjct: 427 NSCSCSCSCSCSIESTL 377
Score = 29.6 bits (65), Expect = 0.21
Identities = 14/28 (50%), Positives = 16/28 (57%)
Frame = -1
Query: 37 ILVNENHYSRFVALDGCSCSCSCSCSAA 64
I NEN + G SCSCSCSCS +
Sbjct: 475 ISANENGGGEPGSNSGNSCSCSCSCSCS 392
>TC89720 similar to GP|1492084|gb|AAC55269.1| MC141R {Molluscum
contagiosum virus subtype 1}, partial (4%)
Length = 382
Score = 30.0 bits (66), Expect = 0.16
Identities = 10/12 (83%), Positives = 11/12 (91%)
Frame = +1
Query: 53 CSCSCSCSCSAA 64
CSCSCSCSC+ A
Sbjct: 82 CSCSCSCSCACA 117
Score = 28.1 bits (61), Expect = 0.61
Identities = 10/15 (66%), Positives = 12/15 (79%)
Frame = +1
Query: 54 SCSCSCSCSAASQLL 68
SCSCSCSCS A ++
Sbjct: 79 SCSCSCSCSCACAVI 123
>BF642460 similar to GP|20197062|gb| putative beta-galactosidase
{Arabidopsis thaliana}, partial (6%)
Length = 417
Score = 28.9 bits (63), Expect = 0.36
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = -1
Query: 53 CSCSCSCSCSAASQ 66
CSCSCSCSC+ Q
Sbjct: 66 CSCSCSCSCTELLQ 25
Score = 26.9 bits (58), Expect = 1.4
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = -1
Query: 54 SCSCSCSCSAASQL 67
SCSCSCSCS L
Sbjct: 69 SCSCSCSCSCTELL 28
>TC83119 similar to GP|13357253|gb|AAK20050.1 putative zinc finger protein
{Oryza sativa (japonica cultivar-group)}, partial (16%)
Length = 421
Score = 28.9 bits (63), Expect = 0.36
Identities = 14/36 (38%), Positives = 20/36 (54%)
Frame = -2
Query: 24 NVQVQDHIDAVSSILVNENHYSRFVALDGCSCSCSC 59
N+ ++H D V+++ FV D CSCSCSC
Sbjct: 318 NLSCKEHHDRN----VDQSESYGFVGYDFCSCSCSC 223
>BM815814 similar to GP|12328527|db hypothetical protein~similar to
Arabidopsis thaliana chromosome 5 K3M16_30, partial
(37%)
Length = 644
Score = 28.1 bits (61), Expect = 0.61
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = +2
Query: 51 DGCSCSCSCSCSAASQ 66
D CSCSCSC C Q
Sbjct: 11 D*CSCSCSCPCHCQEQ 58
>BF632420 weakly similar to GP|21618116|gb| unknown {Arabidopsis thaliana},
partial (17%)
Length = 534
Score = 27.7 bits (60), Expect = 0.80
Identities = 9/10 (90%), Positives = 9/10 (90%)
Frame = -3
Query: 53 CSCSCSCSCS 62
C CSCSCSCS
Sbjct: 142 CCCSCSCSCS 113
>BE324815
Length = 606
Score = 27.3 bits (59), Expect = 1.0
Identities = 13/35 (37%), Positives = 18/35 (51%), Gaps = 1/35 (2%)
Frame = +2
Query: 42 NHYSRFVALDGCSCSCSCS-CSAASQLLNDRWTAA 75
NHY F + C C +CS S+A + D W +A
Sbjct: 272 NHYKCFDSFSSCYCRSNCSWFSSAISISADCWKSA 376
>TC87839 similar to GP|20259677|gb|AAM14356.1 unknown protein {Arabidopsis
thaliana}, partial (84%)
Length = 1160
Score = 27.3 bits (59), Expect = 1.0
Identities = 14/27 (51%), Positives = 15/27 (54%)
Frame = -3
Query: 52 GCSCSCSCSCSAASQLLNDRWTAAIQS 78
GC CSC C CS LL DR + I S
Sbjct: 753 GCGCSCHC-CSRDIILLIDRLNSIITS 676
>TC83798 weakly similar to GP|10177750|dbj|BAB11063. cytochrome P450
{Arabidopsis thaliana}, partial (25%)
Length = 762
Score = 27.3 bits (59), Expect = 1.0
Identities = 9/23 (39%), Positives = 18/23 (78%)
Frame = -2
Query: 54 SCSCSCSCSAASQLLNDRWTAAI 76
+CSCSCSCS+ ++ L+ +++ +
Sbjct: 392 TCSCSCSCSSPTKNLSGIYSSGL 324
Score = 24.6 bits (52), Expect = 6.8
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = -2
Query: 53 CSCSCSCS 60
CSCSCSCS
Sbjct: 389 CSCSCSCS 366
>AL387977
Length = 433
Score = 26.6 bits (57), Expect = 1.8
Identities = 9/12 (75%), Positives = 10/12 (83%)
Frame = +1
Query: 49 ALDGCSCSCSCS 60
A+ CSCSCSCS
Sbjct: 343 AIPSCSCSCSCS 378
Score = 26.2 bits (56), Expect = 2.3
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = +1
Query: 54 SCSCSCSCS 62
SCSCSCSCS
Sbjct: 352 SCSCSCSCS 378
>BF633093 similar to PIR|T47568|T475 fructokinase-like protein - Arabidopsis
thaliana, partial (10%)
Length = 424
Score = 26.6 bits (57), Expect = 1.8
Identities = 18/60 (30%), Positives = 27/60 (45%), Gaps = 4/60 (6%)
Frame = -2
Query: 7 TNSIGNDLEKNQVASSRNVQVQDHIDAVSSILVNENHYSRFV----ALDGCSCSCSCSCS 62
+ SIGN + +S + HI+ SS Y + + + + CSCSCSC S
Sbjct: 393 SGSIGNSCTRTVGRNSLCAAPKQHINGGSS-------YGKSIPSS*SSNSCSCSCSCVVS 235
>BE239772
Length = 347
Score = 26.6 bits (57), Expect = 1.8
Identities = 8/9 (88%), Positives = 8/9 (88%)
Frame = +1
Query: 53 CSCSCSCSC 61
CSCSCSC C
Sbjct: 208 CSCSCSCDC 234
>TC80738 similar to GP|16226347|gb|AAL16142.1 AT3g47850/T23J7_180
{Arabidopsis thaliana}, partial (27%)
Length = 722
Score = 26.6 bits (57), Expect = 1.8
Identities = 12/27 (44%), Positives = 16/27 (58%)
Frame = -1
Query: 39 VNENHYSRFVALDGCSCSCSCSCSAAS 65
+N + S F+ SCSCSCS S+ S
Sbjct: 344 LNSDPSSGFLESTSSSCSCSCSSSSFS 264
>TC78143 homologue to PIR|T47615|T47615 histone H2A.F/Z - Arabidopsis
thaliana, partial (98%)
Length = 723
Score = 26.6 bits (57), Expect = 1.8
Identities = 15/59 (25%), Positives = 27/59 (45%)
Frame = -3
Query: 30 HIDAVSSILVNENHYSRFVALDGCSCSCSCSCSAASQLLNDRWTAAIQSYNNSKDSSEK 88
H+ +SI ++S + +GC CSC CS S L+ ++ + +SS +
Sbjct: 346 HLQIFASISSQLKNFSSQILQNGCQIHCSC-CSDTSISLDSLLQLSVDPTHRKLNSSTR 173
>CA859527
Length = 501
Score = 26.2 bits (56), Expect = 2.3
Identities = 9/17 (52%), Positives = 12/17 (69%)
Frame = +3
Query: 53 CSCSCSCSCSAASQLLN 69
C CS SC+CS + L+N
Sbjct: 132 CDCSSSCNCSNSKVLVN 182
Score = 25.4 bits (54), Expect = 4.0
Identities = 10/33 (30%), Positives = 14/33 (42%)
Frame = +3
Query: 52 GCSCSCSCSCSAASQLLNDRWTAAIQSYNNSKD 84
GC C C CS++ N + S N K+
Sbjct: 111 GCPCGLQCDCSSSCNCSNSKVLVNKASETNPKE 209
>TC88239 homologue to PIR|G96718|G96718 unknown protein 54453-53476
[imported] - Arabidopsis thaliana, partial (33%)
Length = 1649
Score = 26.2 bits (56), Expect = 2.3
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = -3
Query: 54 SCSCSCSCS 62
SCSCSCSCS
Sbjct: 879 SCSCSCSCS 853
Score = 24.6 bits (52), Expect = 6.8
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = -3
Query: 53 CSCSCSCS 60
CSCSCSCS
Sbjct: 876 CSCSCSCS 853
>TC85312 homologue to GP|6164920|gb|AAF04584.1| type 1 metallothionein
{Medicago sativa}, complete
Length = 646
Score = 26.2 bits (56), Expect = 2.3
Identities = 8/15 (53%), Positives = 10/15 (66%)
Frame = +2
Query: 47 FVALDGCSCSCSCSC 61
FV + GC C SC+C
Sbjct: 77 FVNMSGCKCGSSCNC 121
>BQ137654 homologue to GP|6164920|gb|AA type 1 metallothionein {Medicago
sativa}, complete
Length = 782
Score = 26.2 bits (56), Expect = 2.3
Identities = 8/15 (53%), Positives = 10/15 (66%)
Frame = +3
Query: 47 FVALDGCSCSCSCSC 61
FV + GC C SC+C
Sbjct: 48 FVNMSGCKCGSSCNC 92
>TC83065 similar to GP|20258842|gb|AAM13903.1 putative cytochrome P450
{Arabidopsis thaliana}, partial (42%)
Length = 869
Score = 26.2 bits (56), Expect = 2.3
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = +2
Query: 54 SCSCSCSCS 62
SCSCSCSCS
Sbjct: 23 SCSCSCSCS 49
Score = 25.0 bits (53), Expect = 5.2
Identities = 8/11 (72%), Positives = 9/11 (81%)
Frame = +2
Query: 50 LDGCSCSCSCS 60
+ CSCSCSCS
Sbjct: 17 MPSCSCSCSCS 49
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.311 0.121 0.347
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,954,941
Number of Sequences: 36976
Number of extensions: 45408
Number of successful extensions: 830
Number of sequences better than 10.0: 108
Number of HSP's better than 10.0 without gapping: 606
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 783
length of query: 88
length of database: 9,014,727
effective HSP length: 64
effective length of query: 24
effective length of database: 6,648,263
effective search space: 159558312
effective search space used: 159558312
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 51 (24.3 bits)
Lotus: description of TM0336.2