BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0336.16
(135 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BE998261 similar to PIR|T05847|T058 hypothetical protein F17L22.... 223 1e-59
TC87648 similar to GP|17529294|gb|AAL38874.1 unknown protein {Ar... 32 0.076
CA918228 weakly similar to GP|18478606|gb| repetitive proline ri... 30 0.29
TC76627 weakly similar to GP|9294388|dbj|BAB02398.1 cytochrome P... 28 0.84
AJ548069 similar to GP|975305|gb|A SCA 13.1 {synthetic construct... 28 1.4
TC84748 similar to GP|9758300|dbj|BAB08843.1 contains similarity... 28 1.4
TC87806 similar to GP|21592931|gb|AAM64881.1 phosphoserine amino... 28 1.4
TC80608 weakly similar to GP|17064898|gb|AAL32603.1 Unknown prot... 27 2.5
TC89280 similar to GP|13278055|gb|AAH03883.1 Similar to RIKEN cD... 27 2.5
TC93162 similar to PIR|G72453|G72453 hypothetical protein APE227... 27 3.2
TC85752 similar to GP|21706788|gb|AAH34388.1 Unknown (protein fo... 27 3.2
TC87752 similar to GP|10178173|dbj|BAB11647. acyl-CoA oxidase {A... 27 3.2
TC88123 similar to GP|8777484|dbj|BAA97064.1 contains similarity... 27 3.2
AW584704 similar to SP|P05790|FBOH Fibroin heavy chain precursor... 26 4.2
BG648215 SP|P11027|CYD Cytochrome D ubiquinol oxidase subunit II... 26 4.2
AW980849 homologue to GP|7331|emb|CA PYR1-3B multifunctional enz... 26 4.2
TC86556 similar to PIR|T07598|T07598 proline-rich protein GPP1 -... 26 4.2
BG583849 similar to GP|17529352|gb| unknown protein {Arabidopsis... 26 4.2
BQ750853 similar to GP|13562018|gb fibroin 3 {Plectreurys tristi... 26 5.5
CA921605 similar to PIR|F96529|F9 probable Phosphatidylinositol ... 26 5.5
>BE998261 similar to PIR|T05847|T058 hypothetical protein F17L22.180 -
Arabidopsis thaliana, partial (65%)
Length = 488
Score = 223 bits (569), Expect = 1e-59
Identities = 98/138 (71%), Positives = 117/138 (84%), Gaps = 10/138 (7%)
Frame = +1
Query: 1 MMPQQWASPCGNQCTHKYAALTQIPWRVFCKKGCNSDGETWEECLEECDHICYKDPVLKD 60
M+PQQWASPCGN+CT+KYAALT++PWRVFCKKGCNSDGETWEEC+E+C+ +CYKDPVLKD
Sbjct: 43 MIPQQWASPCGNKCTNKYAALTKLPWRVFCKKGCNSDGETWEECVEDCNQLCYKDPVLKD 222
Query: 61 QKWSAYIDRSPGAASYSEECFHACVSGCGYKFEVQQEKAEKICPNRPLKP---------- 110
Q+WSAYIDRSPG+A+YS ECFHACVSG GYK EVQ ++A+K CP+RPLKP
Sbjct: 223 QQWSAYIDRSPGSATYS*ECFHACVSGWGYKCEVQPDEADKGCPSRPLKPEPEPAQKRKT 402
Query: 111 APVQKPKPQPVEQLDPPE 128
PVQK KP V+ +DPP+
Sbjct: 403 KPVQKQKPLHVDPIDPPD 456
>TC87648 similar to GP|17529294|gb|AAL38874.1 unknown protein {Arabidopsis
thaliana}, partial (33%)
Length = 990
Score = 32.0 bits (71), Expect = 0.076
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +1
Query: 28 VFCKKGCNSDGETWEECLEECDHICYKDPVLKDQ 61
V+C GC +G+TW ECD + + L D+
Sbjct: 637 VYCCDGCKDEGDTWSYLCAECDFDLHPNCALGDK 738
>CA918228 weakly similar to GP|18478606|gb| repetitive proline rich protein
{Oryza sativa}, partial (13%)
Length = 303
Score = 30.0 bits (66), Expect = 0.29
Identities = 12/29 (41%), Positives = 16/29 (54%)
Frame = -1
Query: 104 PNRPLKPAPVQKPKPQPVEQLDPPEDMHG 132
PN P KP P+ P P+P + P D+ G
Sbjct: 198 PNPPSKPKPIPAPHPKPAPKPGPNNDV*G 112
>TC76627 weakly similar to GP|9294388|dbj|BAB02398.1 cytochrome P450
{Arabidopsis thaliana}, partial (59%)
Length = 1834
Score = 28.5 bits (62), Expect = 0.84
Identities = 15/35 (42%), Positives = 21/35 (59%), Gaps = 1/35 (2%)
Frame = -1
Query: 100 EKICPNRPLKPAPVQKPKPQPVEQLD-PPEDMHGI 133
EK CP + L PAP K + P L+ PPE++ G+
Sbjct: 1468 EKFCPMQILGPAPNGKYENLPFVALEIPPENLSGL 1364
>AJ548069 similar to GP|975305|gb|A SCA 13.1 {synthetic construct}, partial
(55%)
Length = 539
Score = 27.7 bits (60), Expect = 1.4
Identities = 16/47 (34%), Positives = 20/47 (42%), Gaps = 2/47 (4%)
Frame = -1
Query: 43 ECLEECDHICY--KDPVLKDQKWSAYIDRSPGAASYSEECFHACVSG 87
+CL C IC + P K K A A + S CFH C+ G
Sbjct: 353 QCLTHCR*ICLGPQSPS*KPYKLGALEIGLASAETNSSRCFHHCIRG 213
>TC84748 similar to GP|9758300|dbj|BAB08843.1 contains similarity to unknown
protein~dbj|BAA89593.1~gene_id:MJH22.6 {Arabidopsis
thaliana}, partial (58%)
Length = 848
Score = 27.7 bits (60), Expect = 1.4
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = +3
Query: 29 FCKKGCNSDGETWE 42
FC +GC SD ETWE
Sbjct: 300 FC*QGCRSDVETWE 341
>TC87806 similar to GP|21592931|gb|AAM64881.1 phosphoserine aminotransferase
{Arabidopsis thaliana}, partial (58%)
Length = 1010
Score = 27.7 bits (60), Expect = 1.4
Identities = 14/32 (43%), Positives = 15/32 (46%), Gaps = 5/32 (15%)
Frame = +1
Query: 104 PNRPLKPAPVQKPKPQP-----VEQLDPPEDM 130
P P KP P KPKP P LDPP +
Sbjct: 157 PPPPTKPKPSPKPKPIPQIASSTSPLDPPPSL 252
>TC80608 weakly similar to GP|17064898|gb|AAL32603.1 Unknown protein
{Arabidopsis thaliana}, partial (41%)
Length = 1304
Score = 26.9 bits (58), Expect = 2.5
Identities = 12/28 (42%), Positives = 14/28 (49%)
Frame = -3
Query: 104 PNRPLKPAPVQKPKPQPVEQLDPPEDMH 131
P PL PVQ P+P +Q DP H
Sbjct: 336 PKLPLYSCPVQYPQPSFDQQFDPFHQYH 253
>TC89280 similar to GP|13278055|gb|AAH03883.1 Similar to RIKEN cDNA
2010107D16 gene {Mus musculus}, partial (76%)
Length = 1198
Score = 26.9 bits (58), Expect = 2.5
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = +3
Query: 104 PNRPLKPAPVQKPKPQPVEQLDP 126
PN+P +P P Q P+P E LDP
Sbjct: 240 PNQPQQPQPPQPPQP---ESLDP 299
>TC93162 similar to PIR|G72453|G72453 hypothetical protein APE2275 -
Aeropyrum pernix (strain K1), partial (2%)
Length = 775
Score = 26.6 bits (57), Expect = 3.2
Identities = 14/40 (35%), Positives = 19/40 (47%), Gaps = 4/40 (10%)
Frame = +1
Query: 92 FEVQQEKAEKICPNRPLKPAPVQKP----KPQPVEQLDPP 127
F +Q + PNRP+ P P +KP K +P PP
Sbjct: 115 FMLQSSMEGTLFPNRPVLPLPTKKPTQALKFKPTFSHSPP 234
>TC85752 similar to GP|21706788|gb|AAH34388.1 Unknown (protein for
MGC:34661) {Homo sapiens}, partial (80%)
Length = 827
Score = 26.6 bits (57), Expect = 3.2
Identities = 11/29 (37%), Positives = 14/29 (47%)
Frame = +3
Query: 99 AEKICPNRPLKPAPVQKPKPQPVEQLDPP 127
A + R +PAP P+P PV PP
Sbjct: 81 ARRSSGGRSARPAPRAAPRPAPVNHAPPP 167
>TC87752 similar to GP|10178173|dbj|BAB11647. acyl-CoA oxidase {Arabidopsis
thaliana}, partial (61%)
Length = 1524
Score = 26.6 bits (57), Expect = 3.2
Identities = 20/85 (23%), Positives = 32/85 (37%), Gaps = 16/85 (18%)
Frame = +2
Query: 34 CNSDGETW---EECLEECD------HICYKDPVLKDQKWSAYIDRS-------PGAASYS 77
CNS G W C+ C H C++ W++ RS +Y+
Sbjct: 188 CNSRGTCWW*GRPCIFFCKRPQGVGHYCHQIFSTSSAIWTSRPTRSNDS*LPVSST*AYA 367
Query: 78 EECFHACVSGCGYKFEVQQEKAEKI 102
F+ C+S C YK + E++
Sbjct: 368 NAGFNLCISFCHYKLSGEIRSNEEV 442
>TC88123 similar to GP|8777484|dbj|BAA97064.1 contains similarity to
RNA-binding protein~gene_id:K15M2.15 {Arabidopsis
thaliana}, partial (11%)
Length = 815
Score = 26.6 bits (57), Expect = 3.2
Identities = 12/31 (38%), Positives = 15/31 (47%)
Frame = -1
Query: 97 EKAEKICPNRPLKPAPVQKPKPQPVEQLDPP 127
++A P P +P P P P PV L PP
Sbjct: 251 KEASPCPPLPPAEPCPPPAPTPGPVTPLPPP 159
>AW584704 similar to SP|P05790|FBOH Fibroin heavy chain precursor (Fib-H)
(H-fibroin). [Silk moth] {Bombyx mori}, partial (0%)
Length = 679
Score = 26.2 bits (56), Expect = 4.2
Identities = 10/25 (40%), Positives = 13/25 (52%)
Frame = +1
Query: 111 APVQKPKPQPVEQLDPPEDMHGISA 135
+PV P P P + PP HG+ A
Sbjct: 556 SPVPSPSPTPAPEAAPPSMHHGLLA 630
>BG648215 SP|P11027|CYD Cytochrome D ubiquinol oxidase subunit II (EC
1.10.3.-) (Cytochrome BD-I oxidase subunit II)., partial
(68%)
Length = 802
Score = 26.2 bits (56), Expect = 4.2
Identities = 10/29 (34%), Positives = 18/29 (61%), Gaps = 1/29 (3%)
Frame = +1
Query: 105 NRPLKPAPVQKP-KPQPVEQLDPPEDMHG 132
N + +P+++P + +PV PPE +HG
Sbjct: 469 NHRYRTSPIRQPVRNRPVSPAQPPEPLHG 555
>AW980849 homologue to GP|7331|emb|CA PYR1-3B multifunctional enzyme (1014
AA) {Dictyostelium discoideum}, partial (1%)
Length = 604
Score = 26.2 bits (56), Expect = 4.2
Identities = 12/42 (28%), Positives = 19/42 (44%)
Frame = +1
Query: 62 KWSAYIDRSPGAASYSEECFHACVSGCGYKFEVQQEKAEKIC 103
KW Y+ P +++ +CF C SG + Q K + C
Sbjct: 334 KWLCYVISLPASSTIFVQCFTWCGSGTNF----SQSKRKSTC 447
>TC86556 similar to PIR|T07598|T07598 proline-rich protein GPP1 - potato,
partial (24%)
Length = 1250
Score = 26.2 bits (56), Expect = 4.2
Identities = 14/36 (38%), Positives = 20/36 (54%), Gaps = 4/36 (11%)
Frame = +3
Query: 102 ICPNRPL-KPAPVQKPKPQPV---EQLDPPEDMHGI 133
+ P P+ KP PV KP+P PV + PP+ H +
Sbjct: 240 VLPPAPVYKPPPVPKPQPPPVPVKKPCPPPKVEHPV 347
>BG583849 similar to GP|17529352|gb| unknown protein {Arabidopsis thaliana},
partial (23%)
Length = 799
Score = 26.2 bits (56), Expect = 4.2
Identities = 13/41 (31%), Positives = 23/41 (55%), Gaps = 2/41 (4%)
Frame = +2
Query: 93 EVQQEKAEKICPNRPLKPAPVQKPKPQP--VEQLDPPEDMH 131
+V Q+ +R + P P+Q+PKPQ ++++ PP H
Sbjct: 77 DVVQKLTGSPAHDRIITPPPIQQPKPQSSRLQRIRPPPLPH 199
>BQ750853 similar to GP|13562018|gb fibroin 3 {Plectreurys tristis}, partial
(0%)
Length = 692
Score = 25.8 bits (55), Expect = 5.5
Identities = 11/39 (28%), Positives = 21/39 (53%)
Frame = +3
Query: 95 QQEKAEKICPNRPLKPAPVQKPKPQPVEQLDPPEDMHGI 133
++ + K P R KPA +PKP+ L+P +++ +
Sbjct: 327 RRARPPKTLPTRTAKPA--SRPKPRAAAPLEPEDELSSL 437
>CA921605 similar to PIR|F96529|F9 probable Phosphatidylinositol 4-kinase
PI4K [imported] - Arabidopsis thaliana, partial (6%)
Length = 633
Score = 25.8 bits (55), Expect = 5.5
Identities = 9/20 (45%), Positives = 14/20 (70%)
Frame = +1
Query: 5 QWASPCGNQCTHKYAALTQI 24
QW PC ++ TH A++TQ+
Sbjct: 379 QWDHPC*SKVTHYLASMTQL 438
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.317 0.134 0.462
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,581,941
Number of Sequences: 36976
Number of extensions: 128234
Number of successful extensions: 911
Number of sequences better than 10.0: 64
Number of HSP's better than 10.0 without gapping: 801
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 891
length of query: 135
length of database: 9,014,727
effective HSP length: 86
effective length of query: 49
effective length of database: 5,834,791
effective search space: 285904759
effective search space used: 285904759
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)
Lotus: description of TM0336.16