BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0336.15
(501 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC77627 similar to GP|15028089|gb|AAK76575.1 unknown protein {Ar... 786 0.0
BI271217 similar to GP|15028089|gb unknown protein {Arabidopsis ... 190 7e-84
TC86856 similar to GP|9759387|dbj|BAB10038.1 dihydropyrimidinase... 91 8e-19
TC81993 similar to GP|9759387|dbj|BAB10038.1 dihydropyrimidinase... 59 3e-09
TC82462 similar to GP|9759387|dbj|BAB10038.1 dihydropyrimidinase... 35 0.067
CB891335 homologue to PIR|D64759|D64 yahJ protein - Escherichia ... 34 0.15
TC91853 similar to GP|9955546|emb|CAC05431.1 putative protein {A... 32 0.43
BF004404 similar to GP|21928159|gb AT4g02010/T10M13_2 {Arabidops... 31 0.97
TC77706 similar to GP|14532908|gb|AAK64136.1 unknown protein {Ar... 30 2.8
TC78935 similar to GP|6760451|gb|AAF28357.1| S-ribonuclease bind... 29 3.7
TC87534 similar to SP|P17597|ILVB_ARATH Acetolactate synthase c... 28 6.3
AJ504073 similar to GP|14190419|gb| At2g31610/T9H9.13 {Arabidops... 28 6.3
TC79924 weakly similar to GP|15146278|gb|AAK83622.1 AT5g20070/F2... 28 6.3
>TC77627 similar to GP|15028089|gb|AAK76575.1 unknown protein {Arabidopsis
thaliana}, partial (88%)
Length = 1964
Score = 786 bits (2031), Expect = 0.0
Identities = 382/447 (85%), Positives = 418/447 (93%)
Frame = +1
Query: 55 SEDLEINEGKIVSITEGYGKQGNSKQEVVIDYGEAVIMPGLIDVHVHLDEPGRTEWEGFV 114
S +EINEGKIVSI EGYGK+ NS +V IDYG+AV+MPGLIDVHVHLDEPGRT+WEGFV
Sbjct: 202 SGSVEINEGKIVSIVEGYGKKDNSVHQV-IDYGDAVVMPGLIDVHVHLDEPGRTDWEGFV 378
Query: 115 TGTRAAAAGGVTTVVDMPLNNHPTTVSQETLQLKLEAAENELHVDVGFWGGLVPENALNT 174
TGT+AAAAGGVTTVVDMPLNNHPTT+S+ETL+LKLEAAE++++VDVGFWGGLVPENA NT
Sbjct: 379 TGTQAAAAGGVTTVVDMPLNNHPTTMSKETLELKLEAAEDKIYVDVGFWGGLVPENAQNT 558
Query: 175 SILEGLLSAGVLGLKSFMCPSGINDFPMTTIDHIKEGLSVLAKYRRPLLVHAEIQQDSKN 234
SILEGLL AGVLGLKSFMCPSGI+DFPMTTI+HIKEGLSVLAKYRRPLLVH+EIQQDS++
Sbjct: 559 SILEGLLDAGVLGLKSFMCPSGIDDFPMTTIEHIKEGLSVLAKYRRPLLVHSEIQQDSES 738
Query: 235 NLELKGNDDPRVYSTYLNTRPPSWEEAAIKELVDVTKDTIHGGPLEGAHVHIVHLSDSSV 294
+ EL+GN DP Y TYL+TRPPSWEEAAIKELVD TKDT GG LEGAHVHIVHLSDSS
Sbjct: 739 SSELEGNGDPHSYKTYLDTRPPSWEEAAIKELVDATKDTRIGGSLEGAHVHIVHLSDSSA 918
Query: 295 SLDLIKEAKLRGDSISVETCSHYLAFSSEEIPDGDTRFKCSPPIRDAFNKEKLWEAVLEG 354
SLDLIKEAK RGDSIS+ETC HYL FSSEEIPD DTR+KCSPPIRDA N+EKLWEA+L+G
Sbjct: 919 SLDLIKEAKRRGDSISIETCPHYLTFSSEEIPDKDTRYKCSPPIRDASNREKLWEALLDG 1098
Query: 355 HIDLLTSDHSPTVPELKLLKEGDFLKAWGGISSLQFDLPVTWSYGKKHGLTLEQLSLLWS 414
HIDLL+SDHSPTVP+LKLLKEGDFLKAWGGISSLQFDLPVTWSYGKKHGLTLE+LSLLWS
Sbjct: 1099HIDLLSSDHSPTVPKLKLLKEGDFLKAWGGISSLQFDLPVTWSYGKKHGLTLEKLSLLWS 1278
Query: 415 QKPATLAGIASKGAIVVGNHADIVVWHPELEFELNDDYPVFLKHPNLSAYMGRRLSGKVL 474
QKPA LAG+ SKGAI VGNHADIVVW PE+EF+LNDDYPVF+KHP+LSAYMG RL+GKVL
Sbjct: 1279QKPAKLAGLESKGAIAVGNHADIVVWQPEVEFDLNDDYPVFIKHPSLSAYMGSRLAGKVL 1458
Query: 475 DTFVRGNLVFKDGKHAPAACGVPILAK 501
DTFVRGNLVFKDGKHAPAACGVPILAK
Sbjct: 1459DTFVRGNLVFKDGKHAPAACGVPILAK 1539
>BI271217 similar to GP|15028089|gb unknown protein {Arabidopsis thaliana},
partial (26%)
Length = 626
Score = 190 bits (482), Expect(2) = 7e-84
Identities = 88/100 (88%), Positives = 95/100 (95%)
Frame = +3
Query: 353 EGHIDLLTSDHSPTVPELKLLKEGDFLKAWGGISSLQFDLPVTWSYGKKHGLTLEQLSLL 412
+GHIDLL+SDHSPTVP+LKLLKEGDFLKAWGGISSLQFDLPVTWSYGKKHGLTLE+LSLL
Sbjct: 324 DGHIDLLSSDHSPTVPKLKLLKEGDFLKAWGGISSLQFDLPVTWSYGKKHGLTLEKLSLL 503
Query: 413 WSQKPATLAGIASKGAIVVGNHADIVVWHPELEFELNDDY 452
WSQKPA LAG+ SKGAI VGNHADI VW PE+EF+LNDDY
Sbjct: 504 WSQKPAKLAGLESKGAIAVGNHADIXVWQPEVEFDLNDDY 623
Score = 139 bits (350), Expect(2) = 7e-84
Identities = 66/77 (85%), Positives = 70/77 (90%)
Frame = +2
Query: 276 GGPLEGAHVHIVHLSDSSVSLDLIKEAKLRGDSISVETCSHYLAFSSEEIPDGDTRFKCS 335
GG LEGAHVHIVHLSDSS SLDLIKEAK RGDSIS+ETC HYL FSSEEIPD DTR+KCS
Sbjct: 8 GGSLEGAHVHIVHLSDSSASLDLIKEAKRRGDSISIETCPHYLTFSSEEIPDKDTRYKCS 187
Query: 336 PPIRDAFNKEKLWEAVL 352
PPIRDA N+EKLWEA+L
Sbjct: 188 PPIRDASNREKLWEALL 238
>TC86856 similar to GP|9759387|dbj|BAB10038.1 dihydropyrimidinase
{Arabidopsis thaliana}, partial (90%)
Length = 1911
Score = 91.3 bits (225), Expect = 8e-19
Identities = 113/452 (25%), Positives = 190/452 (42%), Gaps = 17/452 (3%)
Frame = +3
Query: 54 HSEDLEINEGKIVSITEGYGKQGNSKQEVVIDYGEAVIMPGLIDVHVHL--DEPGRTEWE 111
H D+ + +G IV++ VID +MPG ID H HL D G T +
Sbjct: 231 HVADVYVEDGIIVAVQPNIMV---GDDVYVIDATGKFVMPGGIDPHAHLGMDALGTTSVD 401
Query: 112 GFVTGTRAAAAGGVTTVVD--MPLNNHPTTVSQE-TLQLKLEAAENELHVDVGFWGGLVP 168
F +G AA AGG T +D MP+N + T + + K + HV V W
Sbjct: 402 DFFSGQSAALAGGTTMHIDFVMPINGNLTAGFEAYEKKAKNSCMDYGFHVAVTKW----- 566
Query: 169 ENALNTSILEGLLSAGVLGLKSFMCPSGINDFPMTTIDHIKEGLSVLAKYRRPLLVHAE- 227
+ +++T + + G+ K FM +F M + + +G +VHAE
Sbjct: 567 DESVSTDMEIMVKEKGINSFKFFMA---YKEFIMVNDELLLQGFKKCKSLGALAMVHAEN 737
Query: 228 ---IQQDSKNNLELKGNDDPRVYSTYLNTRPPSWEEAAIKELVDVTKDTIHGGPLEGAHV 284
+ + +EL G P + +RPP E A T I +
Sbjct: 738 GDAVYAGQQKMIEL-GITGPEGHPL---SRPPMLEGEA-------TSRAIRLADFVNTPL 884
Query: 285 HIVHLSDSSVSLDLIKEAKLRGDSISVETCSHYLAFSSEEI--PDGDT--RFKCSPPIRD 340
++VH+ S +++ I +A+ G + E LA + PD T ++ SPPIR
Sbjct: 885 YVVHVM-SIDAMEEIAKARKSGQRVIGEPIVSGLALDDSWLWHPDFTTAAKYLMSPPIRS 1061
Query: 341 AFNKEKLWEAVLEGHIDLLTSDHSPTVPELKLLKEGDFLKAWGGISSLQFDLPVTWSYGK 400
+ + L A+ G + L+ +DH K DF + G++ ++ + V W
Sbjct: 1062KGHDKALQSALATGVLQLVGTDHCAWNSTQKARGVDDFRQIINGVNGIEERMHVVWDIMV 1241
Query: 401 KHG-LTLEQLSLLWSQKPATLAGI-ASKGAIVVGNHADIVVWHPELEFELNDDYPVFLKH 458
+ G +++ + S + A + I KGAI+ G+ ADI++ +P FE+ H
Sbjct: 1242ESGQISVTDYVRVTSTECARIFNIYPRKGAILPGSDADIIILNPNSSFEIT----AKSHH 1409
Query: 459 PNL--SAYMGRRLSGKVLDTFVRGNLVFKDGK 488
L + Y G + GK+ T G +V+++ +
Sbjct: 1410SRLDTNVYEGWKGKGKIEVTIAGGRVVWENNE 1505
>TC81993 similar to GP|9759387|dbj|BAB10038.1 dihydropyrimidinase
{Arabidopsis thaliana}, partial (51%)
Length = 1096
Score = 59.3 bits (142), Expect = 3e-09
Identities = 53/211 (25%), Positives = 100/211 (47%), Gaps = 6/211 (2%)
Frame = +3
Query: 284 VHIVHLSDSSVSLDLIKEAKLRGDSISVETCSHYLAFSSEEI--PDGDT--RFKCSPPIR 339
+++VH+ S +++ + +A+ G + E LA + PD DT ++ SPPIR
Sbjct: 78 LYVVHVM-SIDAMEEVAKARKSGQRVIGEPVLSGLALDDSWLWHPDFDTAAKYVMSPPIR 254
Query: 340 DAFNKEKLWEAVLEGHIDLLTSDHSPTVPELKLLKEGDFLKAWGGISSLQFDLPVTWSYG 399
+ + L A+ G + L+ +DH K DF K G++ ++ + V W
Sbjct: 255 KQGHDKALQAALSTGVLQLVGTDHCVFNSTQKAFGIDDFRKIPNGVNGIEERMRVVWDIM 434
Query: 400 KKHG-LTLEQLSLLWSQKPATLAGI-ASKGAIVVGNHADIVVWHPELEFELNDDYPVFLK 457
+ G +++ L S + A + I KGAI+ G+ ADI++ +P F++
Sbjct: 435 VESGQISVTDYVRLTSTECARIFNIYPKKGAILPGSDADIIILNPNSSFDIT--AKSHHS 608
Query: 458 HPNLSAYMGRRLSGKVLDTFVRGNLVFKDGK 488
+ + Y GR+ GK+ T G +V+++ +
Sbjct: 609 RVDTNVYEGRKGKGKIEVTVAGGRVVWENNE 701
>TC82462 similar to GP|9759387|dbj|BAB10038.1 dihydropyrimidinase
{Arabidopsis thaliana}, partial (28%)
Length = 693
Score = 35.0 bits (79), Expect = 0.067
Identities = 30/112 (26%), Positives = 47/112 (41%), Gaps = 5/112 (4%)
Frame = +3
Query: 57 DLEINEGKIVSITEGYGKQGNSKQEVVIDYGEAVIMPGLIDVHVHLDEP--GRTEWEGFV 114
D+ + +G IV++ VID +MPG ID H HL+ + F
Sbjct: 267 DVYVEDGIIVAVNPTIAV---GDDVYVIDATGKFVMPGGIDPHTHLEMEFMNTVAVDDFF 437
Query: 115 TGTRAAAAGGVTTVVDMPL-NNHPTTVSQETLQLKLEAA--ENELHVDVGFW 163
+G AA AGG T +D + N T E + K + + + H+ + W
Sbjct: 438 SGQAAALAGGTTMHIDFVIPTNGSLTAGFEAYEKKAKKSCMDYGFHMAITKW 593
>CB891335 homologue to PIR|D64759|D64 yahJ protein - Escherichia coli,
partial (35%)
Length = 844
Score = 33.9 bits (76), Expect = 0.15
Identities = 23/70 (32%), Positives = 35/70 (49%)
Frame = +2
Query: 35 LLRVGIDFEKRKADPYTLNHSEDLEINEGKIVSITEGYGKQGNSKQEVVIDYGEAVIMPG 94
LL G D+E A T + +EI +GKIV++ E K D G +++P
Sbjct: 536 LLETGFDYENGVA-VQTRTARQTVEIQDGKIVALREN--KLHPDATLPHYDAGGKLMLPT 706
Query: 95 LIDVHVHLDE 104
D+H+HLD+
Sbjct: 707 TRDMHIHLDK 736
>TC91853 similar to GP|9955546|emb|CAC05431.1 putative protein {Arabidopsis
thaliana}, partial (25%)
Length = 667
Score = 32.3 bits (72), Expect = 0.43
Identities = 25/80 (31%), Positives = 35/80 (43%), Gaps = 2/80 (2%)
Frame = -3
Query: 288 HLSDSSVSLDLIKEAKLRG--DSISVETCSHYLAFSSEEIPDGDTRFKCSPPIRDAFNKE 345
HL D+S SL L L G SIS+ SH L+ + + +C+PP+ +
Sbjct: 467 HLGDTSPSLILTLSPTLYGLTRSISLALQSHNLSTKQNDTHKLSSSTRCTPPMLE*LTSP 288
Query: 346 KLWEAVLEGHIDLLTSDHSP 365
+ W V D LT SP
Sbjct: 287 QWWVGV---ETDALTLTQSP 237
>BF004404 similar to GP|21928159|gb AT4g02010/T10M13_2 {Arabidopsis
thaliana}, partial (26%)
Length = 592
Score = 31.2 bits (69), Expect = 0.97
Identities = 26/82 (31%), Positives = 36/82 (43%), Gaps = 14/82 (17%)
Frame = +1
Query: 187 GLKSFMCPSGIN-----DFPMTTIDHIKEGLSVLAKYRRPLLVHAEIQQDS---KNNLEL 238
GL S+ CP GIN D PM GLS L + +P ++H + + +NN
Sbjct: 280 GLASW*CPMGINCPLDWDTPMKIALDAARGLSYLHEDSQPCVIHRDFMAFNILLENNFHA 459
Query: 239 KGND------DPRVYSTYLNTR 254
K D P + YL+TR
Sbjct: 460 KVADFGLAKQAPEGGANYLSTR 525
>TC77706 similar to GP|14532908|gb|AAK64136.1 unknown protein {Arabidopsis
thaliana}, partial (98%)
Length = 2358
Score = 29.6 bits (65), Expect = 2.8
Identities = 19/63 (30%), Positives = 27/63 (42%)
Frame = -2
Query: 201 PMTTIDHIKEGLSVLAKYRRPLLVHAEIQQDSKNNLELKGNDDPRVYSTYLNTRPPSWEE 260
P + HI E YR L + K + KGN+ +++RPP WEE
Sbjct: 1889 PTLMLAHISEKKKCRKSYRTCLNDMVAKRNPVKTRPKAKGNEA-------IDSRPPFWEE 1731
Query: 261 AAI 263
AA+
Sbjct: 1730 AAV 1722
>TC78935 similar to GP|6760451|gb|AAF28357.1| S-ribonuclease binding protein
SBP1 {Petunia x hybrida}, partial (93%)
Length = 1304
Score = 29.3 bits (64), Expect = 3.7
Identities = 26/80 (32%), Positives = 39/80 (48%)
Frame = +1
Query: 368 PELKLLKEGDFLKAWGGISSLQFDLPVTWSYGKKHGLTLEQLSLLWSQKPATLAGIASKG 427
PE K LKE DFL+ ISS+ F P + S G GL+L+ L + A L+
Sbjct: 325 PERKRLKEQDFLENNSQISSVDFLQPRSVSTGL--GLSLDNTRLASTGDSALLS------ 480
Query: 428 AIVVGNHADIVVWHPELEFE 447
++G+ D + +LE +
Sbjct: 481 --LIGDDIDRELQQQDLEMD 534
>TC87534 similar to SP|P17597|ILVB_ARATH Acetolactate synthase chloroplast
precursor (EC 4.1.3.18) (Acetohydroxy-acid synthase)
(ALS)., partial (87%)
Length = 2258
Score = 28.5 bits (62), Expect = 6.3
Identities = 18/60 (30%), Positives = 32/60 (53%)
Frame = +2
Query: 208 IKEGLSVLAKYRRPLLVHAEIQQDSKNNLELKGNDDPRVYSTYLNTRPPSWEEAAIKELV 267
+KE +LA RP V +I +D + + L D P + Y+N P + +EA ++++V
Sbjct: 713 VKEAF-LLASSGRPGPVLIDIPKDIQQQVSLPNWDQPIRLTGYMNRLPKAPDEAHLEQIV 889
>AJ504073 similar to GP|14190419|gb| At2g31610/T9H9.13 {Arabidopsis
thaliana}, partial (26%)
Length = 253
Score = 28.5 bits (62), Expect = 6.3
Identities = 18/59 (30%), Positives = 27/59 (45%)
Frame = -2
Query: 180 LLSAGVLGLKSFMCPSGINDFPMTTIDHIKEGLSVLAKYRRPLLVHAEIQQDSKNNLEL 238
LL+ LG SF G N ++ G+++ ++Y LVH +Q NNL L
Sbjct: 237 LLTKNSLGTSSFDDDLGAN----RGNPNLNTGVTIFSEYTAQKLVHFSVQNSISNNLSL 73
>TC79924 weakly similar to GP|15146278|gb|AAK83622.1 AT5g20070/F28I16_220
{Arabidopsis thaliana}, partial (21%)
Length = 628
Score = 28.5 bits (62), Expect = 6.3
Identities = 19/50 (38%), Positives = 22/50 (44%)
Frame = +1
Query: 364 SPTVPELKLLKEGDFLKAWGGISSLQFDLPVTWSYGKKHGLTLEQLSLLW 413
SPT+P L F GG+S LPV S G GL L +L W
Sbjct: 232 SPTIPTTTLRISRYFRSGMGGLSR----LPVVVSRGDSVGLVLGRLGECW 369
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.316 0.136 0.406
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,342,217
Number of Sequences: 36976
Number of extensions: 211132
Number of successful extensions: 828
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 817
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 824
length of query: 501
length of database: 9,014,727
effective HSP length: 100
effective length of query: 401
effective length of database: 5,317,127
effective search space: 2132167927
effective search space used: 2132167927
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0336.15