BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0334a.8
(635 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC76786 similar to SP|P51615|MAOX_VITVI NADP-dependent malic enz... 988 0.0
TC78502 similar to GP|8118507|gb|AAF73006.1| NADP-dependent mali... 448 0.0
TC83483 homologue to GP|510876|emb|CAA56354.1| NADP dependent ma... 308 e-167
BQ138756 homologue to GP|510876|emb| NADP dependent malic enzyme... 351 4e-97
TC87627 similar to SP|P37221|MAOM_SOLTU NAD-dependent malic enzy... 218 3e-72
BF003636 similar to GP|8894554|emb NAD-dependent malic enzyme (m... 141 9e-34
BG454171 homologue to GP|8894554|emb| NAD-dependent malic enzyme... 60 2e-12
TC81001 similar to SP|P37225|MAON_SOLTU NAD-dependent malic enzy... 64 2e-10
TC82344 similar to SP|P37221|MAOM_SOLTU NAD-dependent malic enzy... 43 3e-04
>TC76786 similar to SP|P51615|MAOX_VITVI NADP-dependent malic enzyme (EC
1.1.1.40) (NADP-ME). [Grape] {Vitis vinifera}, complete
Length = 2039
Score = 988 bits (2555), Expect = 0.0
Identities = 489/592 (82%), Positives = 539/592 (90%)
Frame = +1
Query: 43 KLNIAAMSRNGGEGVVGEDFKNSEESGGVRDLYGEDSATEDQLITPWTFSVASGNTLLRD 102
K+ S GE V+ S GGV D+YGED ATEDQL+TPWTFSVASG +LLRD
Sbjct: 97 KMESTLKSFRDGESVLDLS-PRSAVGGGVEDVYGEDCATEDQLVTPWTFSVASGYSLLRD 273
Query: 103 PRYNKGLAFTEKERDAHYLRGLLPPAVFTQELQEKRTMHNIRQYEVPLHKYMAMMDLQER 162
P+YNKGLAFTEKERDAHYLRGLLPP V +Q+LQEK+ MHNIRQYEVPL KY+AMMDLQER
Sbjct: 274 PQYNKGLAFTEKERDAHYLRGLLPPTVSSQQLQEKKLMHNIRQYEVPLQKYVAMMDLQER 453
Query: 163 NERLFYKILIDNVEELLPIVYTPVVGEACQKYGSIYKRPQGLYISLKEKGKILEVLKNWP 222
NERLFYK+LIDNVEELLPIVYTPVVGEACQKYGSIYKRPQGLYISLKEKGKILEVLKNWP
Sbjct: 454 NERLFYKLLIDNVEELLPIVYTPVVGEACQKYGSIYKRPQGLYISLKEKGKILEVLKNWP 633
Query: 223 EKTIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSSCLPVTIDVGTNNET 282
E++IQVIVVTDGERILGLGDLGCQGMGIPVGKL+LY+ALGGVRPS+CLPVTIDVGTNNE
Sbjct: 634 ERSIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYSALGGVRPSACLPVTIDVGTNNEK 813
Query: 283 LLNDEFYTGLRQKRATGKEYEELLDEFMRAVKQNYGEKVLIQFEDFANHNAFNLLDRYSS 342
LLNDEFY GLRQKRATGKEY +LL EFM AVKQNYGEKVL+QFEDFANHNAF LL +Y +
Sbjct: 814 LLNDEFYIGLRQKRATGKEYYDLLHEFMTAVKQNYGEKVLVQFEDFANHNAFELLAKYGT 993
Query: 343 SHLVFNDDIQGTASVVLAGLLASLKLVGGTLADHTFLFLGAGEAGTGIAELIALEISKQT 402
+HLVFNDDIQGTA+VVLAG++ASLKL+GGTL +HTFLFLGAGEAGTGIAELIALE+SKQT
Sbjct: 994 THLVFNDDIQGTAAVVLAGVVASLKLIGGTLPEHTFLFLGAGEAGTGIAELIALEMSKQT 1173
Query: 403 KAPVEETRKKVWLVDSKGLIVRSRLESLQHFKKPWAHEHEPVKALLDAVKAIKPTVLIGS 462
KAP+EE+RKK+WLVDSKGLIV SR SLQHFKKPWAHEHEPV LLDAVK IKPTVLIGS
Sbjct: 1174KAPIEESRKKIWLVDSKGLIVSSRANSLQHFKKPWAHEHEPVSTLLDAVKIIKPTVLIGS 1353
Query: 463 SGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAEEAYTWSKGKAIFASGSPFDPV 522
SGVGKTFTKEVVEAM +N+ PLILALSNPTSQSECTAEEAYTWS+G+AIFASGSPFDPV
Sbjct: 1354SGVGKTFTKEVVEAMTEINKIPLILALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPV 1533
Query: 523 KYGGKVYAPGQANNAYIFPGFGLGLIISGAIRVRDEMLLAASEALAAQVSQENYDKGLIY 582
+Y GK Y GQ+NNAYIFPGFGLG+++SGAIRV D+MLLAASEALA QV++ENY KGL Y
Sbjct: 1534EYKGKTYYSGQSNNAYIFPGFGLGIVMSGAIRVHDDMLLAASEALAKQVTEENYKKGLTY 1713
Query: 583 PPFTNIRKISAHIAASVAAKVYELGLASHLPRPKDLVKYAESCMYSPGYRSY 634
PPF++IRKISA+IAA+VAAK YELGLA+HLPRP++LVKYAESCMYSP YR+Y
Sbjct: 1714PPFSDIRKISANIAANVAAKAYELGLATHLPRPENLVKYAESCMYSPLYRNY 1869
>TC78502 similar to GP|8118507|gb|AAF73006.1| NADP-dependent malic protein
{Ricinus communis}, partial (65%)
Length = 1540
Score = 448 bits (1153), Expect(3) = 0.0
Identities = 219/266 (82%), Positives = 242/266 (90%)
Frame = +2
Query: 369 VGGTLADHTFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKVWLVDSKGLIVRSRLE 428
VGG LADH FLFLGAGEAGTGIAELIALE SKQT AP++E RK +WLVDSKGLIV SR E
Sbjct: 473 VGGNLADHKFLFLGAGEAGTGIAELIALETSKQTNAPLDEVRKNIWLVDSKGLIVSSRKE 652
Query: 429 SLQHFKKPWAHEHEPVKALLDAVKAIKPTVLIGSSGVGKTFTKEVVEAMASLNEKPLILA 488
SLQHFKKPWAHEHEPVK LLDAV IKPTVLIG+SG G FTKEVVEAMAS+NEKP+IL+
Sbjct: 653 SLQHFKKPWAHEHEPVKNLLDAVNKIKPTVLIGTSGQGSAFTKEVVEAMASINEKPIILS 832
Query: 489 LSNPTSQSECTAEEAYTWSKGKAIFASGSPFDPVKYGGKVYAPGQANNAYIFPGFGLGLI 548
LSNPTSQSECTAEEAYTW++G+AIFASGSPF PV+Y GKV+ PGQANNAYIFPGFGLGLI
Sbjct: 833 LSNPTSQSECTAEEAYTWTQGRAIFASGSPFSPVEYKGKVFVPGQANNAYIFPGFGLGLI 1012
Query: 549 ISGAIRVRDEMLLAASEALAAQVSQENYDKGLIYPPFTNIRKISAHIAASVAAKVYELGL 608
+SG IRV D++LLAASEALA QVS+EN++KGLI+PPFTNIRKISAHIAA VAAK YELGL
Sbjct: 1013MSGTIRVHDDLLLAASEALAEQVSEENFEKGLIFPPFTNIRKISAHIAAKVAAKAYELGL 1192
Query: 609 ASHLPRPKDLVKYAESCMYSPGYRSY 634
A+ LP+PKDLVK+AESCMY+P YRSY
Sbjct: 1193ATRLPQPKDLVKFAESCMYTPAYRSY 1270
Score = 155 bits (392), Expect(3) = 0.0
Identities = 76/98 (77%), Positives = 87/98 (88%)
Frame = +1
Query: 277 GTNNETLLNDEFYTGLRQKRATGKEYEELLDEFMRAVKQNYGEKVLIQFEDFANHNAFNL 336
GTNNE LLNDE Y GL+Q+RATG+EY EL+ EFM AVKQ YGEKVLIQFEDFANHNAF+L
Sbjct: 196 GTNNERLLNDELYIGLKQRRATGQEYSELMHEFMTAVKQTYGEKVLIQFEDFANHNAFDL 375
Query: 337 LDRYSSSHLVFNDDIQGTASVVLAGLLASLKLVGGTLA 374
L++Y S+HLVFNDDIQGTASVVLAGL+A+LKL G L+
Sbjct: 376 LEKYRSTHLVFNDDIQGTASVVLAGLVAALKLGWGKLS 489
Score = 123 bits (309), Expect(3) = 0.0
Identities = 58/64 (90%), Positives = 62/64 (96%)
Frame = +3
Query: 213 KILEVLKNWPEKTIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSSCLPV 272
+I EVL+NWPEK IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPS+CLP+
Sbjct: 3 RIQEVLRNWPEKNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPI 182
Query: 273 TIDV 276
TIDV
Sbjct: 183 TIDV 194
>TC83483 homologue to GP|510876|emb|CAA56354.1| NADP dependent malic enzyme
{Phaseolus vulgaris}, partial (56%)
Length = 1003
Score = 308 bits (789), Expect(3) = e-167
Identities = 152/163 (93%), Positives = 159/163 (97%)
Frame = +3
Query: 384 GEAGTGIAELIALEISKQTKAPVEETRKKVWLVDSKGLIVRSRLESLQHFKKPWAHEHEP 443
G+AGTGIAELIALEISKQTKAPVEETRKK+WLVDSKGLIV SRL+SLQHFKKPWAHEHEP
Sbjct: 414 GKAGTGIAELIALEISKQTKAPVEETRKKIWLVDSKGLIVSSRLQSLQHFKKPWAHEHEP 593
Query: 444 VKALLDAVKAIKPTVLIGSSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAEEA 503
VK LL+AVKAIKPTVLIGSSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAEEA
Sbjct: 594 VKELLNAVKAIKPTVLIGSSGVGKTFTKEVVEAMASLNEKPLILALSNPTSQSECTAEEA 773
Query: 504 YTWSKGKAIFASGSPFDPVKYGGKVYAPGQANNAYIFPGFGLG 546
YTWSKGKAIFASGSPFDPV+Y GKV+ PGQ+NNAYIFPGFGLG
Sbjct: 774 YTWSKGKAIFASGSPFDPVEYEGKVFVPGQSNNAYIFPGFGLG 902
Score = 257 bits (656), Expect(3) = e-167
Identities = 127/137 (92%), Positives = 132/137 (95%)
Frame = +2
Query: 247 GMGIPVGKLSLYTALGGVRPSSCLPVTIDVGTNNETLLNDEFYTGLRQKRATGKEYEELL 306
GMGIPVGKLSLYTALGGVRPSSCLP+TIDVGTNNE LLNDEFY GL QKRATGKEY ELL
Sbjct: 2 GMGIPVGKLSLYTALGGVRPSSCLPITIDVGTNNEKLLNDEFYIGLXQKRATGKEYAELL 181
Query: 307 DEFMRAVKQNYGEKVLIQFEDFANHNAFNLLDRYSSSHLVFNDDIQGTASVVLAGLLASL 366
+EFM AVKQNYGEKVL+QFEDFANHNAF+LLD+YSSSHLVFNDDIQGTASVVLAGLLASL
Sbjct: 182 EEFMHAVKQNYGEKVLVQFEDFANHNAFDLLDKYSSSHLVFNDDIQGTASVVLAGLLASL 361
Query: 367 KLVGGTLADHTFLFLGA 383
KLVGGTLADHTFLFLGA
Sbjct: 362 KLVGGTLADHTFLFLGA 412
Score = 63.9 bits (154), Expect(3) = e-167
Identities = 33/39 (84%), Positives = 34/39 (86%)
Frame = +1
Query: 540 FPGFGLGLIISGAIRVRDEMLLAASEALAAQVSQENYDK 578
FP GLIISGAIRVRDEMLLA SEALAAQVSQENYD+
Sbjct: 883 FPDLVWGLIISGAIRVRDEMLLAXSEALAAQVSQENYDR 999
>BQ138756 homologue to GP|510876|emb| NADP dependent malic enzyme {Phaseolus
vulgaris}, partial (30%)
Length = 680
Score = 351 bits (901), Expect = 4e-97
Identities = 170/189 (89%), Positives = 180/189 (94%)
Frame = +2
Query: 60 EDFKNSEESGGVRDLYGEDSATEDQLITPWTFSVASGNTLLRDPRYNKGLAFTEKERDAH 119
EDF N GGV+DLYGEDSATEDQLITPW FSVASG TLLRDPRYNKGLAFTEKERDAH
Sbjct: 116 EDFVNGV-GGGVKDLYGEDSATEDQLITPWNFSVASGCTLLRDPRYNKGLAFTEKERDAH 292
Query: 120 YLRGLLPPAVFTQELQEKRTMHNIRQYEVPLHKYMAMMDLQERNERLFYKILIDNVEELL 179
Y+RGLLPPAVFTQELQEKR MHN+RQY+VPLHKY+A+MDLQERNERLFYK++IDNVEELL
Sbjct: 293 YVRGLLPPAVFTQELQEKRLMHNLRQYDVPLHKYIALMDLQERNERLFYKLMIDNVEELL 472
Query: 180 PIVYTPVVGEACQKYGSIYKRPQGLYISLKEKGKILEVLKNWPEKTIQVIVVTDGERILG 239
P+VYTP VGEACQKYGSIY+RPQGLYISLKEKGKILEVLKNWPEKTIQVIVVTDGERILG
Sbjct: 473 PVVYTPTVGEACQKYGSIYRRPQGLYISLKEKGKILEVLKNWPEKTIQVIVVTDGERILG 652
Query: 240 LGDLGCQGM 248
LGDLGCQGM
Sbjct: 653 LGDLGCQGM 679
>TC87627 similar to SP|P37221|MAOM_SOLTU NAD-dependent malic enzyme 62 kDa
isoform mitochondrial precursor (EC 1.1.1.39) (NAD-ME).
[Potato], partial (67%)
Length = 1548
Score = 218 bits (556), Expect(2) = 3e-72
Identities = 124/299 (41%), Positives = 176/299 (58%), Gaps = 15/299 (5%)
Frame = +2
Query: 242 DLGCQGMGIPVGKLSLYTALGGVRPSSCLPVTIDVGTNNETLLNDEFYTGLRQKRATGKE 301
DLG QG+GI +GKL LY A G+ P LPV IDVGTNN+ LL D Y GL+Q R G +
Sbjct: 2 DLGVQGIGITIGKLDLYVAAAGINPQRVLPVMIDVGTNNKKLLEDPLYLGLQQHRLDGDD 181
Query: 302 YEELLDEFMRAVKQNYGEKVLIQFEDFANHNAFNLLDRYSSSHLVFNDDIQGTASVVLAG 361
Y ++DEFM AV + V++QFEDF + AF LL RY +++ +FNDD+QGTA V +AG
Sbjct: 182 YLAVIDEFMEAVFTRW-PNVIVQFEDFQSKWAFKLLQRYRTTYRMFNDDVQGTAGVAIAG 358
Query: 362 LLASLKLVGGTLAD---HTFLFLGAGEAGTGIAELIALEISK---QTKAPVEETRKKVWL 415
LL +++ G + D + GAG AG G+ +++ + + + + W+
Sbjct: 359 LLGAVRAQGRPMIDFPKQKIVVAGAGSAGIGVLNAARKTMARMLGNNEVAFQSAKSQFWV 538
Query: 416 VDSKGLIVRSRLESLQHFKKPWAHEHEPV--------KALLDAVKAIKPTVLIGSSGVGK 467
VD+KGLI R E++ P+A + + +L + VK +KP VL+G S VG
Sbjct: 539 VDAKGLITEGR-ENIDPDALPFARNLKEMDRQGLREGASLAEVVKQVKPDVLLGLSAVGG 715
Query: 468 TFTKEVVEAMA-SLNEKPLILALSNPTSQSECTAEEAYTWSKGKAIFASGSPFDPVKYG 525
F+ EV+EA+ S + +P I A+SNPT +ECT +EA++ IFASGSPF V G
Sbjct: 716 LFSNEVLEALKDSTSTRPAIFAMSNPTKNAECTPDEAFSILGDNIIFASGSPFSNVDLG 892
Score = 72.4 bits (176), Expect(2) = 3e-72
Identities = 38/77 (49%), Positives = 51/77 (65%)
Frame = +3
Query: 533 QANNAYIFPGFGLGLIISGAIRVRDEMLLAASEALAAQVSQENYDKGLIYPPFTNIRKIS 592
Q NN Y+FPG GLG ++SG+ V D ML AA+E LAA +S+E KG+I+P + IR I+
Sbjct: 918 QGNNMYLFPGIGLGTLLSGSRIVSDGMLQAAAERLAAYMSEEEVLKGIIFPSISRIRDIT 1097
Query: 593 AHIAASVAAKVYELGLA 609
IAA+V + E LA
Sbjct: 1098KEIAAAVIEEAVEEDLA 1148
>BF003636 similar to GP|8894554|emb NAD-dependent malic enzyme (malate
oxidoreductase) {Cicer arietinum}, partial (66%)
Length = 608
Score = 141 bits (355), Expect = 9e-34
Identities = 74/166 (44%), Positives = 109/166 (65%), Gaps = 2/166 (1%)
Frame = +2
Query: 446 ALLDAVKAIKPTVLIGSSGVGKTFTKEVVEAMA-SLNEKPLILALSNPTSQSECTAEEAY 504
++++ VK + P VL+G SGVG F ++V++AM S++ KP I A+SNPT +ECTA +A+
Sbjct: 29 SIIEVVKKVLPHVLLGLSGVGGVFNEQVLKAMRESVSTKPAIFAMSNPTMNAECTAIDAF 208
Query: 505 TWSKGKAIFASGSPFDPVKYG-GKVYAPGQANNAYIFPGFGLGLIISGAIRVRDEMLLAA 563
+ +FASGSPF+ V G G+ QANN Y+FPG GLG ++SGA + D ML AA
Sbjct: 209 NHAGENIVFASGSPFENVDLGNGRAGHVNQANNMYLFPGIGLGSLLSGAHHITDGMLQAA 388
Query: 564 SEALAAQVSQENYDKGLIYPPFTNIRKISAHIAASVAAKVYELGLA 609
SE LA+ +++E+ KG++YP IR ++A + A+V GLA
Sbjct: 389 SECLASYMTEEDIQKGILYPSIDCIRNVTAEVGAAVLRAAIAEGLA 526
>BG454171 homologue to GP|8894554|emb| NAD-dependent malic enzyme (malate
oxidoreductase) {Cicer arietinum}, partial (26%)
Length = 487
Score = 60.1 bits (144), Expect(2) = 2e-12
Identities = 31/55 (56%), Positives = 38/55 (68%), Gaps = 1/55 (1%)
Frame = +3
Query: 512 IFASGSPFDPVKYG-GKVYAPGQANNAYIFPGFGLGLIISGAIRVRDEMLLAASE 565
+FASGSPF+ V G G+ QANN Y+FPG GLG ++SGA + D ML AASE
Sbjct: 105 VFASGSPFENVDLGNGRAGHVNQANNMYLFPGIGLGSLLSGAHHITDGMLQAASE 269
Score = 30.4 bits (67), Expect(2) = 2e-12
Identities = 12/21 (57%), Positives = 16/21 (76%)
Frame = +1
Query: 479 SLNEKPLILALSNPTSQSECT 499
S++ KP I A+SNPT +ECT
Sbjct: 4 SVSTKPAIFAMSNPTMNAECT 66
>TC81001 similar to SP|P37225|MAON_SOLTU NAD-dependent malic enzyme 59 kDa
isoform mitochondrial precursor (EC 1.1.1.39) (NAD-ME).
[Potato], partial (20%)
Length = 679
Score = 63.5 bits (153), Expect = 2e-10
Identities = 37/92 (40%), Positives = 50/92 (54%), Gaps = 10/92 (10%)
Frame = +1
Query: 96 GNTLLRDPRYNKGLAFTEKERDAHYLRGLLPPAVFTQELQEKRTMHNIRQYE-------- 147
G +L DP +NK F ERD LRGLLPP V + E Q R + + R E
Sbjct: 367 GADILHDPWFNKDTGFPLTERDRLGLRGLLPPRVISFEQQYDRFLDSYRSLEKNTLGQPE 546
Query: 148 --VPLHKYMAMMDLQERNERLFYKILIDNVEE 177
V L K+ + L +RNE L+Y++LIDN++E
Sbjct: 547 NVVSLAKWRILNRLHDRNETLYYRVLIDNIKE 642
>TC82344 similar to SP|P37221|MAOM_SOLTU NAD-dependent malic enzyme 62 kDa
isoform mitochondrial precursor (EC 1.1.1.39) (NAD-ME).
[Potato], partial (5%)
Length = 675
Score = 43.1 bits (100), Expect = 3e-04
Identities = 20/38 (52%), Positives = 25/38 (65%)
Frame = +1
Query: 488 ALSNPTSQSECTAEEAYTWSKGKAIFASGSPFDPVKYG 525
A+SNPT +ECT +EA++ IFASGSPF V G
Sbjct: 1 AMSNPTKNAECTPDEAFSILGDNIIFASGSPFSNVDLG 114
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.318 0.137 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,705,953
Number of Sequences: 36976
Number of extensions: 230963
Number of successful extensions: 1064
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1045
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1057
length of query: 635
length of database: 9,014,727
effective HSP length: 102
effective length of query: 533
effective length of database: 5,243,175
effective search space: 2794612275
effective search space used: 2794612275
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 61 (28.1 bits)
Lotus: description of TM0334a.8