BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0327.8
(250 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC86659 similar to GP|5360263|dbj|BAA81904.1 NtPRp27 {Nicotiana ... 240 3e-64
AW288055 31 0.51
TC89016 similar to GP|19073328|gb|AAL84762.1 typical P-type R2R3... 30 0.67
TC84473 similar to GP|23095415|emb|CAD46621. unknown {Streptococ... 30 1.1
BG583982 28 2.5
TC86450 similar to GP|21537318|gb|AAM61659.1 unknown {Arabidopsi... 28 2.5
TC86451 similar to GP|21537318|gb|AAM61659.1 unknown {Arabidopsi... 27 5.7
TC84621 similar to GP|10176991|dbj|BAB10223. glycosylation enzym... 27 7.4
TC76483 weakly similar to GP|9755390|gb|AAF98197.1| F17F8.22 {Ar... 27 7.4
TC76494 weakly similar to GP|9755390|gb|AAF98197.1| F17F8.22 {Ar... 27 7.4
BE124681 similar to PIR|B86201|B86 protein F12K11.9 [imported] -... 27 9.7
BG451334 weakly similar to GP|9755390|gb| F17F8.22 {Arabidopsis ... 27 9.7
TC86596 similar to PIR|S56648|S56648 trypsin inhibitor precursor... 27 9.7
TC85936 homologue to GP|13537115|dbj|BAB40755. AtSUG1 {Arabidops... 27 9.7
TC79101 weakly similar to GP|9755390|gb|AAF98197.1| F17F8.22 {Ar... 27 9.7
BI309524 weakly similar to GP|9755390|gb| F17F8.22 {Arabidopsis ... 27 9.7
>TC86659 similar to GP|5360263|dbj|BAA81904.1 NtPRp27 {Nicotiana tabacum},
partial (83%)
Length = 875
Score = 240 bits (613), Expect = 3e-64
Identities = 111/221 (50%), Positives = 155/221 (69%)
Frame = +1
Query: 30 LMFLVIIASSGSPIHAVQYQVTNRATGTPGGTRFENEIGIPFTQQTIQLASQFILQTFKQ 89
L F +++ + I AV Y VTN A TPGG RF +++G + QT+ A+QFI + F+Q
Sbjct: 67 LYFFLVLLAIIHGIQAVDYSVTNNALSTPGGVRFRDQLGAQYATQTLDSATQFIWRVFQQ 246
Query: 90 IDQFGGKNIEHVSVIVTALNGNSAAITSDNNIFLDSNYIEGFSGDVRNEVIGILYHEATH 149
+ KN++ VS+ V ++G A TS+N I L + Y+ + GD+R E+ G+LYHE TH
Sbjct: 247 NNPADRKNVQKVSLFVDDMDG--VAYTSNNEIHLSARYVNSYGGDLRKEITGVLYHEMTH 420
Query: 150 IWQWFGNKEAPRGLTEGVADFMRLKAGFAPPHWVPRGTGSGWDEGYVVTAYFLDYCNGLR 209
+WQW GN +A GL EG+AD++RLKA +AP HWV G G+ WD+GY VTA FLDYC+ LR
Sbjct: 421 VWQWNGNGQANGGLIEGIADYVRLKANYAPSHWVKPGQGNKWDQGYDVTARFLDYCDTLR 600
Query: 210 DGFVAMLNAMMKDHYSDDFFVKLLGKSVDQLWSDYKSVFGN 250
GFVA LN +M++ YSD FFV+LLGK+V+QLW++YK+ +GN
Sbjct: 601 SGFVAELNKLMRNGYSDQFFVQLLGKTVNQLWTEYKAKYGN 723
>AW288055
Length = 375
Score = 30.8 bits (68), Expect = 0.51
Identities = 16/50 (32%), Positives = 22/50 (44%)
Frame = +3
Query: 199 AYFLDYCNGLRDGFVAMLNAMMKDHYSDDFFVKLLGKSVDQLWSDYKSVF 248
A L YC GF+ LN MKD + D +LG +L Y + +
Sbjct: 57 ARLLHYCEKYDKGFIQRLNEAMKDTWHDRMVGDVLGLKATKLCGLYNATY 206
>TC89016 similar to GP|19073328|gb|AAL84762.1 typical P-type R2R3 Myb
protein {Sorghum bicolor}, partial (81%)
Length = 1120
Score = 30.4 bits (67), Expect = 0.67
Identities = 21/89 (23%), Positives = 40/89 (44%), Gaps = 2/89 (2%)
Frame = +2
Query: 83 ILQTFKQIDQFGGKNIEHVSVIVTALNGNSAAITSDNNIFLDSNYIEG--FSGDVRNEVI 140
+L+ + QF N + S I+ N ++++ + I DS+ +E S +N+
Sbjct: 563 LLEQIQPAPQFPDTNNPNGSHILLHQNTLQSSVSGISGISSDSSSVEFQVASNSDQNDSS 742
Query: 141 GILYHEATHIWQWFGNKEAPRGLTEGVAD 169
+L HE + +W F N++ G D
Sbjct: 743 ELLGHEGSKLWSSFNNQQVSEQEKSGACD 829
>TC84473 similar to GP|23095415|emb|CAD46621. unknown {Streptococcus
agalactiae}, partial (2%)
Length = 756
Score = 29.6 bits (65), Expect = 1.1
Identities = 10/39 (25%), Positives = 20/39 (50%)
Frame = -1
Query: 139 VIGILYHEATHIWQWFGNKEAPRGLTEGVADFMRLKAGF 177
++G + +W W G +A L +G+ +++ AGF
Sbjct: 120 IVGAIQRAMARVWLWDGRSKASPXLLDGMVEYIAELAGF 4
>BG583982
Length = 868
Score = 28.5 bits (62), Expect = 2.5
Identities = 11/31 (35%), Positives = 18/31 (57%)
Frame = +2
Query: 20 HWTKMSPIIYLMFLVIIASSGSPIHAVQYQV 50
HW + IY++F I S+ S +H + YQ+
Sbjct: 656 HWVNLPIYIYMLFGTAIFSN*SWLHQIDYQI 748
>TC86450 similar to GP|21537318|gb|AAM61659.1 unknown {Arabidopsis
thaliana}, partial (39%)
Length = 1268
Score = 28.5 bits (62), Expect = 2.5
Identities = 30/99 (30%), Positives = 51/99 (51%)
Frame = -2
Query: 15 AALIYHWTKMSPIIYLMFLVIIASSGSPIHAVQYQVTNRATGTPGGTRFENEIGIPFTQQ 74
AALI+ K+ P LV I+S+ P+H Q + TG+ GG+ + I
Sbjct: 823 AALIHDLQKI*P-----HLVDISSTIGPMHIGQVK-----TGSFGGSGGFLTLSIVL--- 683
Query: 75 TIQLASQFILQTFKQIDQFGGKNIEHVSVIVTALNGNSA 113
+ LAS+FI ++ +ID+FG + + +++ L +SA
Sbjct: 682 -LLLASKFI*RSQLKIDRFGDSRLRLLRLLLLNLICSSA 569
>TC86451 similar to GP|21537318|gb|AAM61659.1 unknown {Arabidopsis
thaliana}, partial (51%)
Length = 995
Score = 27.3 bits (59), Expect = 5.7
Identities = 28/94 (29%), Positives = 48/94 (50%)
Frame = -3
Query: 15 AALIYHWTKMSPIIYLMFLVIIASSGSPIHAVQYQVTNRATGTPGGTRFENEIGIPFTQQ 74
AALI+ K+ P LV I+S+ P+H Q + TG+ GG+ + I
Sbjct: 735 AALIHDLQKI*P-----HLVDISSTIGPMHIGQVK-----TGSFGGSGGFLTLSIVL--- 595
Query: 75 TIQLASQFILQTFKQIDQFGGKNIEHVSVIVTAL 108
+ LAS+FI ++ +ID+FG + + +++ L
Sbjct: 594 -LLLASKFI*RSQLKIDRFGDSRLRLLRLLLLNL 496
>TC84621 similar to GP|10176991|dbj|BAB10223. glycosylation enzyme-like
protein {Arabidopsis thaliana}, partial (42%)
Length = 709
Score = 26.9 bits (58), Expect = 7.4
Identities = 13/29 (44%), Positives = 19/29 (64%)
Frame = +3
Query: 5 LPICCILSSCAALIYHWTKMSPIIYLMFL 33
LP+CC++ CA+L+ S II L+FL
Sbjct: 630 LPLCCVVYICASLV------SMIIELVFL 698
>TC76483 weakly similar to GP|9755390|gb|AAF98197.1| F17F8.22 {Arabidopsis
thaliana}, partial (61%)
Length = 1632
Score = 26.9 bits (58), Expect = 7.4
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = +1
Query: 187 TGSGWDEGYVVTAYFLDY 204
TGS WDEG + +F DY
Sbjct: 520 TGSAWDEGAEASQWFSDY 573
>TC76494 weakly similar to GP|9755390|gb|AAF98197.1| F17F8.22 {Arabidopsis
thaliana}, partial (65%)
Length = 754
Score = 26.9 bits (58), Expect = 7.4
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = +3
Query: 187 TGSGWDEGYVVTAYFLDY 204
TGS WDEG + +F DY
Sbjct: 417 TGSAWDEGAEASQWFSDY 470
>BE124681 similar to PIR|B86201|B86 protein F12K11.9 [imported] - Arabidopsis
thaliana, partial (25%)
Length = 558
Score = 26.6 bits (57), Expect = 9.7
Identities = 26/126 (20%), Positives = 53/126 (41%), Gaps = 24/126 (19%)
Frame = +2
Query: 25 SPIIYLMFLVIIASSGS---------PIHAVQYQVTNRATGTPGGTRFENEIGIPFTQQT 75
SP++ L+FLV+ +S S P + + + T + T +F+ +IG
Sbjct: 65 SPLVLLLFLVLEHASNSLNYLYQYSWPAYFTKVKFTRQILNTLSN*QFKKQIGCKNKNSK 244
Query: 76 IQLASQFILQTFKQIDQFGGKNIEHV---------------SVIVTALNGNSAAITSDNN 120
+ ++ I+ +Q +++ I+ V S ++ ++N NS T N
Sbjct: 245 PNI*NKLIIMKSRQ*NEYYIDMIQRVHFIPISNRLSENLKNSTLIISINQNSKLFTL-LN 421
Query: 121 IFLDSN 126
+F+ N
Sbjct: 422 LFITQN 439
>BG451334 weakly similar to GP|9755390|gb| F17F8.22 {Arabidopsis thaliana},
partial (48%)
Length = 653
Score = 26.6 bits (57), Expect = 9.7
Identities = 10/18 (55%), Positives = 11/18 (60%)
Frame = +3
Query: 187 TGSGWDEGYVVTAYFLDY 204
TGS WDEG +F DY
Sbjct: 369 TGSAWDEGAEAAQWFSDY 422
>TC86596 similar to PIR|S56648|S56648 trypsin inhibitor precursor (clone
ATI21) - alfalfa, partial (95%)
Length = 590
Score = 26.6 bits (57), Expect = 9.7
Identities = 17/40 (42%), Positives = 22/40 (54%)
Frame = +2
Query: 5 LPICCILSSCAALIYHWTKMSPIIYLMFLVIIASSGSPIH 44
LP+ I SS +L +W KMS + M L I SS P+H
Sbjct: 239 LPLHKINSSSVSLC*YWRKMSLSLQKMPLHKIFSSTVPLH 358
>TC85936 homologue to GP|13537115|dbj|BAB40755. AtSUG1 {Arabidopsis
thaliana}, partial (98%)
Length = 1633
Score = 26.6 bits (57), Expect = 9.7
Identities = 19/58 (32%), Positives = 33/58 (56%), Gaps = 4/58 (6%)
Frame = -2
Query: 87 FKQIDQ---FGGKN-IEHVSVIVTALNGNSAAITSDNNIFLDSNYIEGFSGDVRNEVI 140
F QI+Q F GKN ++H++V+ + N + + D NIF++ N I F D+ + +
Sbjct: 594 FHQINQWINFAGKN*MKHITVVAKS-NSSRWSNLCDVNIFININNIFPFWMDLDQDFV 424
>TC79101 weakly similar to GP|9755390|gb|AAF98197.1| F17F8.22 {Arabidopsis
thaliana}, partial (76%)
Length = 1119
Score = 26.6 bits (57), Expect = 9.7
Identities = 10/18 (55%), Positives = 11/18 (60%)
Frame = +2
Query: 187 TGSGWDEGYVVTAYFLDY 204
TGS WDEG +F DY
Sbjct: 365 TGSAWDEGAEAAQWFSDY 418
>BI309524 weakly similar to GP|9755390|gb| F17F8.22 {Arabidopsis thaliana},
partial (33%)
Length = 640
Score = 26.6 bits (57), Expect = 9.7
Identities = 10/18 (55%), Positives = 11/18 (60%)
Frame = +3
Query: 187 TGSGWDEGYVVTAYFLDY 204
TGS WDEG +F DY
Sbjct: 243 TGSAWDEGAEAAQWFSDY 296
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.323 0.139 0.438
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,030,373
Number of Sequences: 36976
Number of extensions: 103011
Number of successful extensions: 587
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 586
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 586
length of query: 250
length of database: 9,014,727
effective HSP length: 94
effective length of query: 156
effective length of database: 5,538,983
effective search space: 864081348
effective search space used: 864081348
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0327.8