BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0327.6
(442 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC82674 weakly similar to GP|19698923|gb|AAL91197.1 unknown prot... 162 3e-40
AW697042 similar to GP|13122418|dbj putative glycin-rich protein... 30 1.4
BQ151174 similar to GP|11036868|gb| PxORF73 peptide {Plutella xy... 30 2.4
AW697201 similar to GP|12484199|gb| auxin response transcription... 30 2.4
BQ144271 weakly similar to GP|21322711|em pherophorin-dz1 protei... 29 4.1
AW689987 weakly similar to GP|1911338|gb|A unknown {Orgyia pseud... 29 4.1
BQ144325 weakly similar to GP|13161443|dbj putative extensin-lik... 29 4.1
BF640516 similar to GP|17065312|gb Unknown protein {Arabidopsis ... 29 4.1
BQ141742 weakly similar to PIR|T10798|T107 pherophorin-S - Volvo... 28 5.4
TC80366 similar to GP|13877617|gb|AAK43886.1 protein kinase-like... 28 5.4
BQ143922 28 5.4
TC79471 similar to GP|21536629|gb|AAM60961.1 seed maturation-lik... 28 5.4
BG648462 weakly similar to GP|14253163|em VMP4 protein {Volvox c... 28 5.4
BQ144502 weakly similar to GP|6523547|emb hydroxyproline-rich gl... 28 7.1
TC85985 similar to GP|5139695|dbj|BAA81686.1 expressed in cucumb... 28 7.1
TC82244 similar to GP|22507458|gb|AAH19429.1 Unknown (protein fo... 28 7.1
BQ135716 homologue to GP|13879785|gb| hypothetical protein {Myco... 28 7.1
AW682928 similar to GP|22507458|gb| Unknown (protein for MGC:303... 28 7.1
TC79079 similar to GP|19698893|gb|AAL91182.1 splicing factor pu... 28 7.1
TC79498 similar to GP|8885583|dbj|BAA97513.1 gb|AAF35419.1~gene_... 28 7.1
>TC82674 weakly similar to GP|19698923|gb|AAL91197.1 unknown protein
{Arabidopsis thaliana}, partial (13%)
Length = 670
Score = 162 bits (409), Expect = 3e-40
Identities = 105/202 (51%), Positives = 124/202 (60%), Gaps = 7/202 (3%)
Frame = +2
Query: 1 GCLVMDI*APQLSGLER--VRLTTQVRAS-E*GQQLNSLNRTQSRANLRVRLRRGGDIHR 57
G LVMDI* PQLS +E V LTT+VR + NS +QSRAN VRLR ++H
Sbjct: 68 GYLVMDI*TPQLSRVEEL*V*LTTKVRTC*RWT*RFNSFYLSQSRANFWVRLRSLSNLHG 247
Query: 58 VLARLPPIQHPKASLGIREMQPFSR*PKPLPSSAPPTPPGNHIPLTHRAR*HLPKIPAPE 117
+L RLP IQH K SL REM R P P + PTPP NHI LTH AR*HLP IPA +
Sbjct: 248 ILTRLPSIQHSKTSLDFREMHTLFRKP*-FPPTPSPTPPRNHIQLTHSAR*HLP*IPASK 424
Query: 118 TEAGTHIMDHGLTHTGISLQFLQPRLHRAPLLALRL*RAQRSWVTLLPIPARSHSRDGVV 177
T+ H MDH L HT ISL FLQP H + LLA+ L RA RS TL P+ RS SR+ VV
Sbjct: 425 TQTRAHCMDHRLAHTRISLHFLQPCFHHSSLLAMCLRRA*RSRFTLFPLVTRSGSRNCVV 604
Query: 178 ED----PQNVLPHRRSRHRALF 195
++ +N+L +R RH LF
Sbjct: 605 KETSFGAKNLLYYRXCRH*TLF 670
>AW697042 similar to GP|13122418|dbj putative glycin-rich protein {Oryza
sativa (japonica cultivar-group)}, partial (11%)
Length = 730
Score = 30.4 bits (67), Expect = 1.4
Identities = 21/63 (33%), Positives = 24/63 (37%)
Frame = -2
Query: 79 PFSR*PKPLPSSAPPTPPGNHIPLTHRAR*HLPKIPAPETEAGTHIMDHGLTHTGISLQF 138
P R P P P P PP +P + HL K P P T + H TH S Q
Sbjct: 726 PARRPPPPTPPPRPTPPPYPPLPYSPTTSSHLSKPPPPPTP--PPLPPHPPTHYSASSQC 553
Query: 139 LQP 141
P
Sbjct: 552 PAP 544
Score = 29.3 bits (64), Expect = 3.2
Identities = 24/72 (33%), Positives = 30/72 (41%), Gaps = 6/72 (8%)
Frame = -3
Query: 88 PSSAPPTPPGNHIPLTHRAR*HLPKIPAPETEAGTHIMDHGLTHTGISL------QFLQP 141
P +APP PP + PL H P +P P T + H H + L L P
Sbjct: 725 PHAAPPPPPLHLAPLPLPTL-HSPTLPPPPL---TSLSPHPHPHLPLCLLTPPPTTLLAP 558
Query: 142 RLHRAPLLALRL 153
R PLL L+L
Sbjct: 557 SAPRRPLLLLQL 522
>BQ151174 similar to GP|11036868|gb| PxORF73 peptide {Plutella xylostella
granulovirus}, partial (42%)
Length = 909
Score = 29.6 bits (65), Expect = 2.4
Identities = 19/57 (33%), Positives = 24/57 (41%), Gaps = 12/57 (21%)
Frame = +3
Query: 75 REMQPFSR*PKPLPSSAPPTPPG----------NHIPL--THRAR*HLPKIPAPETE 119
++ +P R P P+ APP PP H P TH R H P P P T+
Sbjct: 114 KKEKPPPRPATPPPAPAPPGPPNQTTNTTNPKKQHTPTTPTHPPRPHTPPTPPPTTQ 284
>AW697201 similar to GP|12484199|gb| auxin response transcription factor 3
{Arabidopsis thaliana}, partial (5%)
Length = 770
Score = 29.6 bits (65), Expect = 2.4
Identities = 24/81 (29%), Positives = 36/81 (43%), Gaps = 10/81 (12%)
Frame = -1
Query: 45 LRVRLRRGGDIHRVLARLPPIQHPKASLGIREMQPFSR*--PKPLPSS--------APPT 94
LR RR ++R A P I R+ +P+SR P LP S +P +
Sbjct: 662 LRPPARRPCSVYRARALAPSITR-------RDRRPYSRARPPSRLPPSQSHPPASPSPSS 504
Query: 95 PPGNHIPLTHRAR*HLPKIPA 115
PP + + +H+A H P P+
Sbjct: 503 PPPSSVSTSHQASPHQPHHPS 441
>BQ144271 weakly similar to GP|21322711|em pherophorin-dz1 protein {Volvox
carteri f. nagariensis}, partial (5%)
Length = 919
Score = 28.9 bits (63), Expect = 4.1
Identities = 24/78 (30%), Positives = 32/78 (40%), Gaps = 9/78 (11%)
Frame = +2
Query: 51 RGGDIHRVLARLPPIQHPKASLGIREMQPFSR*PK---------PLPSSAPPTPPGNHIP 101
RGG + R P + P ASL P P P P +PPTPP
Sbjct: 47 RGGRVPPTPPRTPQFR-PGASLTHLHTSPTPHAPPLVPRRPRRGPFPPPSPPTPPIRLPT 223
Query: 102 LTHRAR*HLPKIPAPETE 119
T +++ H P +P P T+
Sbjct: 224 PTTQSKPH-PPVPLPNTK 274
>AW689987 weakly similar to GP|1911338|gb|A unknown {Orgyia pseudotsugata
single capsid nuclear polyhedrosis virus}, partial (11%)
Length = 675
Score = 28.9 bits (63), Expect = 4.1
Identities = 27/92 (29%), Positives = 36/92 (38%), Gaps = 3/92 (3%)
Frame = +1
Query: 36 LNRTQSRANLRVRLR---RGGDIHRVLARLPPIQHPKASLGIREMQPFSR*PKPLPSSAP 92
L R Q+R N + RG + R L R PP L P P P P+ +
Sbjct: 55 LKRDQTRKNAAITTAVTLRGLSLRRNLRRPPPSLRLSPLLPPLSPTPI---PPPTPALSS 225
Query: 93 PTPPGNHIPLTHRAR*HLPKIPAPETEAGTHI 124
PTP IP+ P IP+ + + T I
Sbjct: 226 PTPSPMKIPIYPP-----PPIPSTNSTSSTSI 306
>BQ144325 weakly similar to GP|13161443|dbj putative extensin-like protein
{Oryza sativa (japonica cultivar-group)}, partial (3%)
Length = 438
Score = 28.9 bits (63), Expect = 4.1
Identities = 20/47 (42%), Positives = 24/47 (50%), Gaps = 3/47 (6%)
Frame = +1
Query: 62 LPPIQHPKASLGIREMQPFSR*PKPLPS---SAPPTPPGNHIPLTHR 105
LPPI A+L +R F+ P P PS S PP PP PL+ R
Sbjct: 208 LPPI----AALWVRATYTFNIAPDPPPSTSVSPPPPPPLTPSPLSFR 336
>BF640516 similar to GP|17065312|gb Unknown protein {Arabidopsis thaliana},
partial (30%)
Length = 659
Score = 28.9 bits (63), Expect = 4.1
Identities = 18/55 (32%), Positives = 25/55 (44%), Gaps = 3/55 (5%)
Frame = +2
Query: 65 IQHPKASLGIREMQPFSR*PKPL---PSSAPPTPPGNHIPLTHRAR*HLPKIPAP 116
I+HP ++ ++ P S+ P P S P TPP I TH H P + P
Sbjct: 2 IRHPTSAYPHSQISPTSQITPPTTTKPKSRPQTPPQLQILQTHHPPHHHPFLRRP 166
>BQ141742 weakly similar to PIR|T10798|T107 pherophorin-S - Volvox carteri,
partial (9%)
Length = 1337
Score = 28.5 bits (62), Expect = 5.4
Identities = 18/53 (33%), Positives = 21/53 (38%), Gaps = 7/53 (13%)
Frame = -2
Query: 51 RGGDIHRVLARLPPIQHPKASLGI-------REMQPFSR*PKPLPSSAPPTPP 96
RGGD + PP P L + QP + P PLP PP PP
Sbjct: 184 RGGDEKTI*KAQPPHALPSPPLRSFPWLAPHSQTQPATHSPPPLPPPPPPIPP 26
>TC80366 similar to GP|13877617|gb|AAK43886.1 protein kinase-like protein
{Arabidopsis thaliana}, partial (16%)
Length = 814
Score = 28.5 bits (62), Expect = 5.4
Identities = 15/37 (40%), Positives = 17/37 (45%)
Frame = +3
Query: 84 PKPLPSSAPPTPPGNHIPLTHRAR*HLPKIPAPETEA 120
P PSS PP+ P N P+T P P P T A
Sbjct: 12 PPATPSSPPPSTPANPPPVT-------PSAPPPSTPA 101
>BQ143922
Length = 999
Score = 28.5 bits (62), Expect = 5.4
Identities = 21/59 (35%), Positives = 25/59 (41%), Gaps = 1/59 (1%)
Frame = +3
Query: 63 PPIQHPKASLGIREMQPFS-R*PKPLPSSAPPTPPGNHIPLTHRAR*HLPKIPAPETEA 120
PP +HP I P + R P P TPP H P H ++ H P P P T A
Sbjct: 468 PPARHPSRIPNIHNECPTNTRNPHD-----PDTPP-RHNPHRHISQYHTPAPPTPPTSA 626
>TC79471 similar to GP|21536629|gb|AAM60961.1 seed maturation-like protein
{Arabidopsis thaliana}, partial (50%)
Length = 1464
Score = 28.5 bits (62), Expect = 5.4
Identities = 15/50 (30%), Positives = 25/50 (50%)
Frame = +2
Query: 53 GDIHRVLARLPPIQHPKASLGIREMQPFSR*PKPLPSSAPPTPPGNHIPL 102
G I+ ++ L P+ H + + + P S P PL PP+PP + + L
Sbjct: 86 GSINNFISTLHPLTHHQ-----QPISPISPPPSPLSLLPPPSPPPSTLSL 220
>BG648462 weakly similar to GP|14253163|em VMP4 protein {Volvox carteri f.
nagariensis}, partial (4%)
Length = 678
Score = 28.5 bits (62), Expect = 5.4
Identities = 14/34 (41%), Positives = 16/34 (46%)
Frame = +1
Query: 63 PPIQHPKASLGIREMQPFSR*PKPLPSSAPPTPP 96
PP HP + + E QP P P P S PP P
Sbjct: 466 PPHPHPPSQPPLPETQPPLVPPPPPPGSPPPPSP 567
>BQ144502 weakly similar to GP|6523547|emb hydroxyproline-rich glycoprotein
DZ-HRGP {Volvox carteri f. nagariensis}, partial (39%)
Length = 1358
Score = 28.1 bits (61), Expect = 7.1
Identities = 27/84 (32%), Positives = 35/84 (41%), Gaps = 1/84 (1%)
Frame = -3
Query: 15 LERVRLTTQVRAS-E*GQQLNSLNRTQSRANLRVRLRRGGDIHRVLARLPPIQHPKASLG 73
L R R T + R+S Q+ L R+ RA R R R R PP + +AS
Sbjct: 894 LTRARSTARRRSSTSPAPQIPPLRRSIVRA----RAARAVRPPRTAQRHPPRRTLRASAA 727
Query: 74 IREMQPFSR*PKPLPSSAPPTPPG 97
+ R P P P P +PPG
Sbjct: 726 QHPSRAPRRHPPPPPPPPPSSPPG 655
>TC85985 similar to GP|5139695|dbj|BAA81686.1 expressed in cucumber
hypocotyls {Cucumis sativus}, partial (42%)
Length = 892
Score = 28.1 bits (61), Expect = 7.1
Identities = 15/36 (41%), Positives = 20/36 (54%)
Frame = +3
Query: 60 ARLPPIQHPKASLGIREMQPFSR*PKPLPSSAPPTP 95
A +P + P AS + + P S PKP P S+PP P
Sbjct: 294 APVPVAKSPAASSPV--VAPVSTPPKPAPVSSPPAP 395
>TC82244 similar to GP|22507458|gb|AAH19429.1 Unknown (protein for
MGC:30371) {Mus musculus}, partial (13%)
Length = 847
Score = 28.1 bits (61), Expect = 7.1
Identities = 15/38 (39%), Positives = 20/38 (52%), Gaps = 4/38 (10%)
Frame = +2
Query: 163 LLPIPARSHSRDGVVED----PQNVLPHRRSRHRALFH 196
LL P RS S + + P+++ PHRRSR R H
Sbjct: 290 LLQHPNRSSSINSITRPEYNVPRSLSPHRRSRRRCFLH 403
>BQ135716 homologue to GP|13879785|gb| hypothetical protein {Mycobacterium
tuberculosis CDC1551}, partial (3%)
Length = 935
Score = 28.1 bits (61), Expect = 7.1
Identities = 14/29 (48%), Positives = 17/29 (58%), Gaps = 1/29 (3%)
Frame = -1
Query: 84 PKPL-PSSAPPTPPGNHIPLTHRAR*HLP 111
P PL P PPTPP H TH++ H+P
Sbjct: 146 PPPL*PQDPPPTPPRTH---THQSYSHVP 69
>AW682928 similar to GP|22507458|gb| Unknown (protein for MGC:30371) {Mus
musculus}, partial (13%)
Length = 641
Score = 28.1 bits (61), Expect = 7.1
Identities = 15/38 (39%), Positives = 20/38 (52%), Gaps = 4/38 (10%)
Frame = +1
Query: 163 LLPIPARSHSRDGVVED----PQNVLPHRRSRHRALFH 196
LL P RS S + + P+++ PHRRSR R H
Sbjct: 55 LLQHPNRSSSINSITRPEYNVPRSLSPHRRSRRRCFLH 168
>TC79079 similar to GP|19698893|gb|AAL91182.1 splicing factor putative
{Arabidopsis thaliana}, partial (17%)
Length = 1114
Score = 28.1 bits (61), Expect = 7.1
Identities = 18/57 (31%), Positives = 22/57 (38%)
Frame = +1
Query: 63 PPIQHPKASLGIREMQPFSR*PKPLPSSAPPTPPGNHIPLTHRAR*HLPKIPAPETE 119
P HP S G+ M P P P PP PP P LP+ P P+ +
Sbjct: 274 PYAPHPHPSSGMMPMMH----PPPPPQGVPPPPPPEEAPPP------LPEEPEPKRQ 414
>TC79498 similar to GP|8885583|dbj|BAA97513.1
gb|AAF35419.1~gene_id:F24B18.2~strong similarity to
unknown protein {Arabidopsis thaliana}, partial (79%)
Length = 1269
Score = 28.1 bits (61), Expect = 7.1
Identities = 15/35 (42%), Positives = 17/35 (47%)
Frame = -2
Query: 62 LPPIQHPKASLGIREMQPFSR*PKPLPSSAPPTPP 96
L P QHP ++ S P PL SS PP PP
Sbjct: 839 LGPTQHPH-----HQVSSLSPSPPPLSSSPPPPPP 750
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.325 0.141 0.445
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,236,585
Number of Sequences: 36976
Number of extensions: 236744
Number of successful extensions: 2900
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 2064
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2813
length of query: 442
length of database: 9,014,727
effective HSP length: 99
effective length of query: 343
effective length of database: 5,354,103
effective search space: 1836457329
effective search space used: 1836457329
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 60 (27.7 bits)
Lotus: description of TM0327.6