BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0322.4
(153 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC92044 similar to GP|20260634|gb|AAM13215.1 unknown protein {Ar... 212 2e-69
TC83211 similar to GP|5803275|dbj|BAA83585.1 EST AU056133(S20320... 112 5e-43
TC86072 similar to GP|11559260|dbj|BAB18760. beta-cyanoalanine s... 31 0.22
TC83855 similar to PIR|A45690|A45690 transactivator EBNA-2 - bab... 26 5.5
BQ144475 26 7.2
TC88537 weakly similar to GP|21536818|gb|AAM61150.1 F-box protei... 25 9.3
TC78563 weakly similar to GP|20259502|gb|AAM13871.1 putative ATP... 25 9.3
TC77144 similar to GP|20160594|dbj|BAB89541. contains ESTs AU031... 25 9.3
>TC92044 similar to GP|20260634|gb|AAM13215.1 unknown protein {Arabidopsis
thaliana}, partial (44%)
Length = 969
Score = 212 bits (540), Expect(2) = 2e-69
Identities = 102/112 (91%), Positives = 103/112 (91%)
Frame = +2
Query: 6 IMEIQTSGRPIESLLEKVLCMNILSSDYFKELYRLKTYLEVIDEIYNQVDHVEPWMAGNC 65
IMEIQTSGRPIESLLEKVLCMNILSSDYFKELYRLKTY EVIDEIYNQVDHVEPWM GNC
Sbjct: 428 IMEIQTSGRPIESLLEKVLCMNILSSDYFKELYRLKTYHEVIDEIYNQVDHVEPWMTGNC 607
Query: 66 RGPSTFFCLLY*FFTMMLTVKQMHGLLKHPDSPYIRVVGFLYLRYVPDPNTV 117
RGPST FCLLY FFTM LTVKQMHGLLKHPDSPYIR VGFLYLRY DP T+
Sbjct: 608 RGPSTAFCLLYKFFTMKLTVKQMHGLLKHPDSPYIRAVGFLYLRYYADPKTL 763
Score = 66.2 bits (160), Expect(2) = 2e-69
Identities = 31/38 (81%), Positives = 35/38 (91%)
Frame = +1
Query: 115 NTV*LV*TIYKG**GIFSWI*WTNDYNGCIYP*FAPWT 152
++V LV* I KG**GIFSWI*WT+DY+GCIYP*FAPWT
Sbjct: 757 DSVELV*AICKG**GIFSWI*WTDDYDGCIYP*FAPWT 870
>TC83211 similar to GP|5803275|dbj|BAA83585.1 EST AU056133(S20320)
corresponds to a region of the predicted gene.~Similar
to Caenorhabditis, partial (22%)
Length = 690
Score = 112 bits (280), Expect(2) = 5e-43
Identities = 55/58 (94%), Positives = 55/58 (94%)
Frame = +1
Query: 6 IMEIQTSGRPIESLLEKVLCMNILSSDYFKELYRLKTYLEVIDEIYNQVDHVEPWMAG 63
IMEIQTSGRPIESLLEKVLCMNILSSDYFKELYRLKTY EVIDEIYNQVDHVEPW G
Sbjct: 193 IMEIQTSGRPIESLLEKVLCMNILSSDYFKELYRLKTYHEVIDEIYNQVDHVEPWDDG 366
Score = 78.2 bits (191), Expect(2) = 5e-43
Identities = 36/44 (81%), Positives = 36/44 (81%)
Frame = +2
Query: 61 MAGNCRGPSTFFCLLY*FFTMMLTVKQMHGLLKHPDSPYIRVVG 104
M GNCRGPST FCLLY FF M LTVKQMHGLLKHPDSPYI G
Sbjct: 359 MTGNCRGPSTAFCLLYKFFPMKLTVKQMHGLLKHPDSPYIXXGG 490
>TC86072 similar to GP|11559260|dbj|BAB18760. beta-cyanoalanine synthase
{Solanum tuberosum}, partial (96%)
Length = 1636
Score = 30.8 bits (68), Expect = 0.22
Identities = 14/27 (51%), Positives = 16/27 (58%)
Frame = -1
Query: 91 LLKHPDSPYIRVVGFLYLRYVPDPNTV 117
LL +PYI +GF LRY P P TV
Sbjct: 883 LLSDGSTPYILTLGFCDLRYCPTPETV 803
>TC83855 similar to PIR|A45690|A45690 transactivator EBNA-2 - baboon
lymphotropic virus, partial (3%)
Length = 574
Score = 26.2 bits (56), Expect = 5.5
Identities = 15/39 (38%), Positives = 20/39 (50%)
Frame = -2
Query: 22 KVLCMNILSSDYFKELYRLKTYLEVIDEIYNQVDHVEPW 60
K +C++ L S E Y L+ LE E+YN H E W
Sbjct: 357 KTICLSHLISRV--EFYILRLTLEDSSEVYNLSCHNEAW 247
>BQ144475
Length = 839
Score = 25.8 bits (55), Expect = 7.2
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = +1
Query: 94 HPDSPYIRVVGFLYLRYVPDP 114
HP SP+ R Y+R +PDP
Sbjct: 676 HPSSPHARSNPQTYIRPIPDP 738
>TC88537 weakly similar to GP|21536818|gb|AAM61150.1 F-box protein family
AtFBW2 {Arabidopsis thaliana}, partial (52%)
Length = 1395
Score = 25.4 bits (54), Expect = 9.3
Identities = 13/47 (27%), Positives = 22/47 (46%)
Frame = -1
Query: 25 CMNILSSDYFKELYRLKTYLEVIDEIYNQVDHVEPWMAGNCRGPSTF 71
C N + + +E Y K + +NQ+ H+ PW + +G S F
Sbjct: 723 CTNFDTIAHIQESYHRKFSSNLF---HNQIAHIAPW*SQTPKGTSIF 592
>TC78563 weakly similar to GP|20259502|gb|AAM13871.1 putative ATP-dependent
RNA helicase {Arabidopsis thaliana}, partial (30%)
Length = 1920
Score = 25.4 bits (54), Expect = 9.3
Identities = 15/61 (24%), Positives = 29/61 (46%)
Frame = -1
Query: 30 SSDYFKELYRLKTYLEVIDEIYNQVDHVEPWMAGNCRGPSTFFCLLY*FFTMMLTVKQMH 89
++D + L TY+ + +I+ + H P + N S+ FC+ + TM T+ +
Sbjct: 1647 NNDNESSTFNLPTYV*CLMKIFQGLGHFLPTLFKN*DEWSSMFCIAFC*ETMQNTIDTLT 1468
Query: 90 G 90
G
Sbjct: 1467 G 1465
>TC77144 similar to GP|20160594|dbj|BAB89541. contains ESTs AU031508(E61760)
AU068322(C20031) AU068323(C20031)~similar to Arabidopsis
thaliana, partial (46%)
Length = 817
Score = 25.4 bits (54), Expect = 9.3
Identities = 15/36 (41%), Positives = 21/36 (57%)
Frame = +1
Query: 7 MEIQTSGRPIESLLEKVLCMNILSSDYFKELYRLKT 42
M+++ G+ + L+K M IL FKEL RLKT
Sbjct: 229 MKMRQLGKFPRTNLKKNQKMKILRQKVFKELLRLKT 336
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.352 0.162 0.599
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,257,808
Number of Sequences: 36976
Number of extensions: 74527
Number of successful extensions: 594
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 589
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 594
length of query: 153
length of database: 9,014,727
effective HSP length: 88
effective length of query: 65
effective length of database: 5,760,839
effective search space: 374454535
effective search space used: 374454535
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.9 bits)
S2: 54 (25.4 bits)
Lotus: description of TM0322.4