BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0318.14
(58 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BQ146584 similar to PIR|T06751|T0675 hypothetical protein F15B8.... 88 8e-19
BG584058 weakly similar to PIR|T48558|T485 hypothetical protein ... 37 0.002
BI271718 similar to GP|18377835|gb| At1g24120/F3I6_4 {Arabidopsi... 37 0.002
TC88171 similar to OMNI|EF0720 chloride channel {Enterococcus fa... 37 0.002
TC80410 37 0.002
TC85948 similar to PIR|T01736|T01736 ribosomal protein L3 precur... 37 0.002
AJ498000 homologue to GP|15073150|emb PUTATIVE CYTOCHROME C TRAN... 34 0.010
TC91576 34 0.010
BF633815 similar to GP|5616142|gb|A unknown {TT virus}, partial ... 34 0.010
TC81604 similar to PIR|B72702|B72702 hypothetical protein APE103... 32 0.038
TC90415 similar to GP|21615421|emb|CAD33930. arabinogalactan pro... 32 0.050
BG455469 31 0.11
TC79513 weakly similar to GP|9885806|gb|AAG01535.1| stellacyanin... 30 0.14
TC83685 29 0.32
TC86237 similar to GP|14517454|gb|AAK62617.1 AT3g02540/F16B3_17 ... 26 2.7
TC76341 similar to GP|7528270|gb|AAF63202.1| poly(A)-binding pro... 26 2.7
TC89690 weakly similar to PIR|G84643|G84643 similar to early nod... 26 3.6
TC85017 weakly similar to GP|9758059|dbj|BAB08638.1 amino acid t... 26 3.6
BQ144287 similar to PIR|G86292|G86 hypothetical protein AAF82153... 26 3.6
TC81034 similar to GP|20453162|gb|AAM19822.1 At5g56885 {Arabidop... 26 3.6
>BQ146584 similar to PIR|T06751|T0675 hypothetical protein F15B8.120 -
Arabidopsis thaliana, partial (37%)
Length = 415
Score = 87.8 bits (216), Expect = 8e-19
Identities = 49/60 (81%), Positives = 53/60 (87%), Gaps = 3/60 (5%)
Frame = +3
Query: 1 MDMKKVTCVVLIVAASMSAALA-TNEVP--APAPGPASGASATVVGSLVGASVLSFFALF 57
M+MKKVTC VL+ A SMSAALA T+EVP APAPGPASGAS TVVGSLVGAS+LSFFALF
Sbjct: 60 MNMKKVTCAVLVAALSMSAALAATHEVPSPAPAPGPASGASTTVVGSLVGASLLSFFALF 239
>BG584058 weakly similar to PIR|T48558|T485 hypothetical protein F14F18.160 -
Arabidopsis thaliana, partial (11%)
Length = 651
Score = 37.0 bits (84), Expect = 0.002
Identities = 22/52 (42%), Positives = 35/52 (67%), Gaps = 3/52 (5%)
Frame = +1
Query: 9 VVLIVAASMSAALATNEVPAPAPGPASGASATVVGS--LVGAS-VLSFFALF 57
+V+I AA++++A + APAPGP +GA+ +V S ++G S VLS A+F
Sbjct: 97 MVIIFAATVASAQNLSPSLAPAPGPDAGAAGSVTSSVAMIGVSIVLSMLAIF 252
>BI271718 similar to GP|18377835|gb| At1g24120/F3I6_4 {Arabidopsis thaliana},
partial (3%)
Length = 635
Score = 37.0 bits (84), Expect = 0.002
Identities = 22/52 (42%), Positives = 35/52 (67%), Gaps = 3/52 (5%)
Frame = +1
Query: 9 VVLIVAASMSAALATNEVPAPAPGPASGASATVVGS--LVGAS-VLSFFALF 57
+V+I AA++++A + APAPGP +GA+ +V S ++G S VLS A+F
Sbjct: 229 MVIIFAATVASAQNLSPSLAPAPGPDAGAAGSVTSSVAMIGVSIVLSMLAIF 384
>TC88171 similar to OMNI|EF0720 chloride channel {Enterococcus faecalis
V583}, partial (3%)
Length = 583
Score = 37.0 bits (84), Expect = 0.002
Identities = 23/52 (44%), Positives = 35/52 (67%), Gaps = 3/52 (5%)
Frame = +2
Query: 9 VVLIVAASMSAALATNEVPAPAPGPASGASATVVGS--LVGAS-VLSFFALF 57
+V+I AA++++A + APAPGP +GA+ +V S +VG S VLS A+F
Sbjct: 119 LVIIFAATVASAQDLSPSLAPAPGPDAGAAGSVTSSVAMVGISVVLSMLAIF 274
>TC80410
Length = 492
Score = 36.6 bits (83), Expect = 0.002
Identities = 22/50 (44%), Positives = 34/50 (68%), Gaps = 3/50 (6%)
Frame = +2
Query: 11 LIVAASMSAALATNEVPAPAPGPASGASATVVGS--LVGAS-VLSFFALF 57
+IV A++++A + APAPGP +G + +V GS ++GAS VLS A+F
Sbjct: 113 VIVFAAVASAQDLSPSLAPAPGPDAGVAGSVAGSVAMIGASIVLSMLAIF 262
>TC85948 similar to PIR|T01736|T01736 ribosomal protein L3 precursor
chloroplast - common tobacco (fragment), partial (84%)
Length = 1531
Score = 36.6 bits (83), Expect = 0.002
Identities = 21/52 (40%), Positives = 35/52 (66%), Gaps = 3/52 (5%)
Frame = +1
Query: 9 VVLIVAASMSAALATNEVPAPAPGPASGASATVVGS--LVGAS-VLSFFALF 57
+V+I AA++++A + APAPGP +GA+ ++ S ++G S VLS A+F
Sbjct: 1060 LVIIFAATVASAQDLSPSLAPAPGPDAGAAGSITSSAAMIGVSIVLSMLAIF 1215
>AJ498000 homologue to GP|15073150|emb PUTATIVE CYTOCHROME C TRANSMEMBRANE
PROTEIN {Sinorhizobium meliloti}, partial (4%)
Length = 552
Score = 34.3 bits (77), Expect = 0.010
Identities = 20/51 (39%), Positives = 34/51 (66%), Gaps = 3/51 (5%)
Frame = +2
Query: 10 VLIVAASMSAALATNEVPAPAPGPASGASATVVGS--LVGAS-VLSFFALF 57
V++ AA++++A + APAP P +GA+ +V S ++GAS +LS A+F
Sbjct: 119 VVVFAAAVASAQDLSPSLAPAPDPDAGAAGSVTNSVAMIGASIILSMLAIF 271
>TC91576
Length = 677
Score = 34.3 bits (77), Expect = 0.010
Identities = 22/51 (43%), Positives = 33/51 (64%), Gaps = 3/51 (5%)
Frame = +2
Query: 10 VLIVAASMSAALATNEVPAPAPGPASGASATVVGS--LVGAS-VLSFFALF 57
V ++AAS+++A + +PAPGP +GA+ V S +VG S VLS A+F
Sbjct: 119 VAMLAASIASAQDLSPSLSPAPGPDAGAAGYVTNSVTMVGVSIVLSMLAIF 271
>BF633815 similar to GP|5616142|gb|A unknown {TT virus}, partial (1%)
Length = 423
Score = 34.3 bits (77), Expect = 0.010
Identities = 22/51 (43%), Positives = 32/51 (62%), Gaps = 3/51 (5%)
Frame = +1
Query: 10 VLIVAASMSAALATNEVPAPAPGPASGASATVVGS--LVGAS-VLSFFALF 57
V I AA +++A + APAPGP +GA+ +V S ++G S VLS A+F
Sbjct: 64 VAIFAAIVASAQDLSPSLAPAPGPDAGAAGSVASSVAMIGVSIVLSMLAIF 216
>TC81604 similar to PIR|B72702|B72702 hypothetical protein APE1033 -
Aeropyrum pernix (strain K1), partial (13%)
Length = 551
Score = 32.3 bits (72), Expect = 0.038
Identities = 23/50 (46%), Positives = 31/50 (62%), Gaps = 3/50 (6%)
Frame = +1
Query: 10 VLIVAASMSAALATNEVPAPAPGPASGASATVVGS--LVGAS-VLSFFAL 56
V+I AAS+ +A + APA GP GA+ +V S ++GAS VLS AL
Sbjct: 121 VVIFAASLVSAQDLSPSLAPASGPDVGAAGSVTSSVAMIGASIVLSMLAL 270
>TC90415 similar to GP|21615421|emb|CAD33930. arabinogalactan protein {Cicer
arietinum}, complete
Length = 421
Score = 32.0 bits (71), Expect = 0.050
Identities = 15/46 (32%), Positives = 24/46 (51%)
Frame = +2
Query: 11 LIVAASMSAALATNEVPAPAPGPASGASATVVGSLVGASVLSFFAL 56
++V M AA + + APAP P S A+ V +++ + V F L
Sbjct: 110 MVVVVMMMAASSVSAADAPAPSPTSDATTLFVPTIIASFVALLFGL 247
>BG455469
Length = 550
Score = 30.8 bits (68), Expect = 0.11
Identities = 21/57 (36%), Positives = 32/57 (55%), Gaps = 3/57 (5%)
Frame = +1
Query: 3 MKKVTCVVLIVAASMSAALATNEVPAPAPGPASGASATVVGS--LVGAS-VLSFFAL 56
MK + ++ A +++A + APAPGP GA +V S ++GAS VLS A+
Sbjct: 82 MKFFSFFFVVFVAVVASAQDLSPSSAPAPGPDVGACGSVTSSVAMIGASIVLSMLAI 252
>TC79513 weakly similar to GP|9885806|gb|AAG01535.1| stellacyanin-like
protein CASLP1 precursor {Capsicum annuum}, partial
(33%)
Length = 1093
Score = 30.4 bits (67), Expect = 0.14
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = +3
Query: 14 AASMSAALATNEVPAPAPGPASGASATVVGSLVG 47
AA+ + A +T P PAP PA+G VG +G
Sbjct: 885 AATPAPAPSTTASPTPAPAPATGRVTYTVGDTIG 986
>TC83685
Length = 424
Score = 29.3 bits (64), Expect = 0.32
Identities = 13/29 (44%), Positives = 17/29 (57%)
Frame = +3
Query: 27 PAPAPGPASGASATVVGSLVGASVLSFFA 55
P PG ASGAS+ V ++V + FFA
Sbjct: 279 PVSGPGAASGASSLKVSAVVAVAAAGFFA 365
>TC86237 similar to GP|14517454|gb|AAK62617.1 AT3g02540/F16B3_17
{Arabidopsis thaliana}, partial (74%)
Length = 1641
Score = 26.2 bits (56), Expect = 2.7
Identities = 12/29 (41%), Positives = 16/29 (54%)
Frame = +1
Query: 27 PAPAPGPASGASATVVGSLVGASVLSFFA 55
PAPAP P S A+AT + G + + A
Sbjct: 598 PAPAPAPISSATATEGSDVYGQAASNLVA 684
Score = 24.6 bits (52), Expect = 7.9
Identities = 12/26 (46%), Positives = 17/26 (65%)
Frame = +1
Query: 15 ASMSAALATNEVPAPAPGPASGASAT 40
A+ +A A+ P+PAP PA +SAT
Sbjct: 556 AATAAPPASVAAPSPAPAPAPISSAT 633
>TC76341 similar to GP|7528270|gb|AAF63202.1| poly(A)-binding protein
{Cucumis sativus}, partial (90%)
Length = 2501
Score = 26.2 bits (56), Expect = 2.7
Identities = 11/35 (31%), Positives = 17/35 (48%)
Frame = +3
Query: 16 SMSAALATNEVPAPAPGPASGASATVVGSLVGASV 50
SM+ ++ PAP P P++G V V S+
Sbjct: 198 SMAQIQVQHQTPAPVPAPSNGVVPNVANQFVTTSL 302
>TC89690 weakly similar to PIR|G84643|G84643 similar to early nodulins
[imported] - Arabidopsis thaliana, partial (56%)
Length = 732
Score = 25.8 bits (55), Expect = 3.6
Identities = 16/54 (29%), Positives = 25/54 (45%)
Frame = +3
Query: 4 KKVTCVVLIVAASMSAALATNEVPAPAPGPASGASATVVGSLVGASVLSFFALF 57
+K+T VV+ + +A + P+PA ASA GA+VL +F
Sbjct: 357 QKLTVVVMSLKGGSRPIVAFSPSPSPAEVEGPAASAVAPAPTSGAAVLQGGGVF 518
>TC85017 weakly similar to GP|9758059|dbj|BAB08638.1 amino acid
transporter-like protein {Arabidopsis thaliana}, partial
(11%)
Length = 481
Score = 25.8 bits (55), Expect = 3.6
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 2/34 (5%)
Frame = +1
Query: 21 LATNEVPAPAP--GPASGASATVVGSLVGASVLS 52
L N +P P P G SGA + ++VGA ++S
Sbjct: 172 LQENPIPDPIPQNGTVSGAVFNISTTMVGAGIMS 273
>BQ144287 similar to PIR|G86292|G86 hypothetical protein AAF82153.1 [imported]
- Arabidopsis thaliana, partial (4%)
Length = 1176
Score = 25.8 bits (55), Expect = 3.6
Identities = 9/29 (31%), Positives = 19/29 (65%)
Frame = -3
Query: 5 KVTCVVLIVAASMSAALATNEVPAPAPGP 33
+++C+ L A+ ++ LA + +P+P P P
Sbjct: 1153 RLSCLALADASRATSTLARHPLPSPPPAP 1067
>TC81034 similar to GP|20453162|gb|AAM19822.1 At5g56885 {Arabidopsis
thaliana}, partial (35%)
Length = 1645
Score = 25.8 bits (55), Expect = 3.6
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = +2
Query: 26 VPAPAPGPASGASATVVGSLVG 47
+P P+P + AS T GSL+G
Sbjct: 800 IPRPSPPSLTKASGTATGSLIG 865
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.319 0.128 0.353
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,392,603
Number of Sequences: 36976
Number of extensions: 12224
Number of successful extensions: 200
Number of sequences better than 10.0: 47
Number of HSP's better than 10.0 without gapping: 185
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 197
length of query: 58
length of database: 9,014,727
effective HSP length: 34
effective length of query: 24
effective length of database: 7,757,543
effective search space: 186181032
effective search space used: 186181032
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)
Lotus: description of TM0318.14