BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0314.16
(185 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG454079 similar to GP|10176956|dbj contains similarity to nonsp... 176 5e-45
TC87440 similar to GP|17979494|gb|AAL50083.1 AT5g64080/MHJ24_6 {... 167 2e-42
TC78493 weakly similar to GP|15010698|gb|AAK74008.1 AT3g22600/F1... 70 6e-13
TC87425 weakly similar to GP|20161478|dbj|BAB90402. contains EST... 67 3e-12
TC79365 weakly similar to GP|15010698|gb|AAK74008.1 AT3g22600/F1... 67 5e-12
BF520250 weakly similar to GP|3128176|gb| unknown protein {Arabi... 59 8e-10
TC90904 similar to GP|3128176|gb|AAC16080.1| unknown protein {Ar... 56 9e-09
TC78186 weakly similar to GP|3128176|gb|AAC16080.1| unknown prot... 52 2e-07
TC81134 weakly similar to GP|14334664|gb|AAK59510.1 unknown prot... 50 5e-07
TC79217 weakly similar to GP|21553541|gb|AAM62634.1 lipid transf... 50 7e-07
TC77556 weakly similar to SP|Q43766|NLT3_HORVU Nonspecific lipid... 47 3e-06
TC76731 similar to GP|13937151|gb|AAK50069.1 AT5g57180/MUL3_13 {... 45 2e-05
TC88967 weakly similar to GP|18477856|emb|CAC86258. lipid transf... 44 4e-05
TC86318 similar to GP|15451110|gb|AAK96826.1 Unknown protein {Ar... 40 5e-04
TC86319 similar to GP|15451110|gb|AAK96826.1 Unknown protein {Ar... 40 7e-04
TC77633 similar to GP|6648215|gb|AAF21213.1| unknown protein {Ar... 34 0.029
TC81257 similar to GP|9279781|dbj|BAB01433.1 glucosyltransferase... 33 0.049
TC83386 similar to GP|14334664|gb|AAK59510.1 unknown protein {Ar... 33 0.049
TC76832 weakly similar to GP|21592534|gb|AAM64483.1 unknown {Ara... 33 0.084
AJ548147 similar to GP|15010698|gb| AT3g22600/F16J14_17 {Arabido... 33 0.084
>BG454079 similar to GP|10176956|dbj contains similarity to nonspecific
lipid-transfer protein~gene_id:MHJ24.6 {Arabidopsis
thaliana}, partial (50%)
Length = 418
Score = 176 bits (446), Expect = 5e-45
Identities = 87/119 (73%), Positives = 100/119 (83%)
Frame = +1
Query: 1 MASKVSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQG 60
MASK+SLIL V+AI +D + A ++ APAPS DC+NL+L M DCLSFVTN ST TKP+G
Sbjct: 19 MASKLSLILFVLAIWTLDSSQGALTHQAPAPSVDCTNLVLTMADCLSFVTNGSTTTKPEG 198
Query: 61 QCCSGLKSVLKTAPSCLCEAFKSSSQFGVVLNVTKALALPSACKVSASNSVTNCGCEFF 119
CCSGLKSVLKTAPSCLCEAFKSS+QFGVVLNVTKA +LP+ACKVSA S T CGC+FF
Sbjct: 199 TCCSGLKSVLKTAPSCLCEAFKSSAQFGVVLNVTKATSLPAACKVSAP-SATKCGCKFF 372
>TC87440 similar to GP|17979494|gb|AAL50083.1 AT5g64080/MHJ24_6 {Arabidopsis
thaliana}, partial (48%)
Length = 818
Score = 167 bits (424), Expect = 2e-42
Identities = 84/115 (73%), Positives = 96/115 (83%)
Frame = +1
Query: 1 MASKVSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQG 60
MASK+SLIL V+AI +D + A ++ APAPS DC+NL+L M DCLSFVTN ST TKP+G
Sbjct: 31 MASKLSLILFVLAIWTLDSSQGALTHQAPAPSVDCTNLVLTMADCLSFVTNGSTTTKPEG 210
Query: 61 QCCSGLKSVLKTAPSCLCEAFKSSSQFGVVLNVTKALALPSACKVSASNSVTNCG 115
CCSGLKSVLKTAPSCLCEAFKSS+QFGVVLNVTKA +LP+ACKVSA S T CG
Sbjct: 211 TCCSGLKSVLKTAPSCLCEAFKSSAQFGVVLNVTKATSLPAACKVSAP-SATKCG 372
>TC78493 weakly similar to GP|15010698|gb|AAK74008.1 AT3g22600/F16J14_17
{Arabidopsis thaliana}, partial (57%)
Length = 797
Score = 69.7 bits (169), Expect = 6e-13
Identities = 37/104 (35%), Positives = 62/104 (59%), Gaps = 1/104 (0%)
Frame = +2
Query: 3 SKVSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQC 62
SK+++ L +V I + +A S+ C+N+++ ++ CL+++T +S+ P C
Sbjct: 80 SKMNMNLVLVVIAMMCAGATAQSS--------CTNVLVSLSPCLNYITGNSST--PSSGC 229
Query: 63 CSGLKSVLKTAPSCLCEAFKS-SSQFGVVLNVTKALALPSACKV 105
CS L SV+ + P CLC+ +S G+ +N T+ALALP ACKV
Sbjct: 230 CSNLASVVSSQPLCLCQVLGGGASSLGISINQTQALALPGACKV 361
>TC87425 weakly similar to GP|20161478|dbj|BAB90402. contains ESTs
C74501(E31745) AU094804(E31745)~unknown protein, partial
(24%)
Length = 929
Score = 67.4 bits (163), Expect = 3e-12
Identities = 34/82 (41%), Positives = 50/82 (60%), Gaps = 3/82 (3%)
Frame = +1
Query: 28 APAPSAD-CSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLKSVLKTAPSCLCEAFKSSS- 85
AP+P+ D C + M+DCL+FV + S +TKP CC L ++ P CLC+ S++
Sbjct: 184 APSPADDGCLMALTNMSDCLTFVEDGSKLTKPDKGCCPELAGLIDGNPICLCKLLGSNTA 363
Query: 86 -QFGVVLNVTKALALPSACKVS 106
FG+ +NV KAL LP+ C V+
Sbjct: 364 DSFGIKINVNKALKLPTICGVT 429
>TC79365 weakly similar to GP|15010698|gb|AAK74008.1 AT3g22600/F16J14_17
{Arabidopsis thaliana}, partial (51%)
Length = 783
Score = 66.6 bits (161), Expect = 5e-12
Identities = 34/96 (35%), Positives = 55/96 (56%), Gaps = 1/96 (1%)
Frame = +2
Query: 11 VVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLKSVL 70
++ + V A+ + N A + C++ I ++ CL+++ S+ P CCS L SV+
Sbjct: 71 ILFLVMVLVANMCTQNEA---QSGCTSTITSLSPCLNYIMGSSS--NPSSSCCSQLSSVV 235
Query: 71 KTAPSCLCEAFKSS-SQFGVVLNVTKALALPSACKV 105
+++P CLC S FG+ +N T AL+LPSACKV
Sbjct: 236 QSSPQCLCSLLNGGGSSFGITINQTLALSLPSACKV 343
>BF520250 weakly similar to GP|3128176|gb| unknown protein {Arabidopsis
thaliana}, partial (69%)
Length = 541
Score = 59.3 bits (142), Expect = 8e-10
Identities = 31/84 (36%), Positives = 46/84 (53%), Gaps = 2/84 (2%)
Frame = +3
Query: 33 ADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLKSVLKTAPSCLCEAFK--SSSQFGVV 90
A+C+N +L + CL FVTN + P CC+G+K V + CLC K G+
Sbjct: 120 AECTNKLLTLAGCLPFVTNQAK--SPTIDCCTGVKEVADKSKRCLCILIKDHDDPNLGLT 293
Query: 91 LNVTKALALPSACKVSASNSVTNC 114
+NVT AL LP+ C ++ ++T C
Sbjct: 294 INVTLALKLPNDC--NSPTNITQC 359
>TC90904 similar to GP|3128176|gb|AAC16080.1| unknown protein {Arabidopsis
thaliana}, partial (28%)
Length = 672
Score = 55.8 bits (133), Expect = 9e-09
Identities = 29/80 (36%), Positives = 42/80 (52%), Gaps = 3/80 (3%)
Frame = +3
Query: 34 DCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLKSVLKTAPSCLCEAFKSSSQ---FGVV 90
+C+ + + CL +V + P CC GLK++LKT CLC K + G+V
Sbjct: 156 ECTAQLTGLASCLPYVEGEGKTPAPD--CCDGLKTLLKTNKKCLCVIIKDRNDPDLGGIV 329
Query: 91 LNVTKALALPSACKVSASNS 110
+NVT AL LP+ C A+ S
Sbjct: 330 INVTLALNLPTVCNAPANIS 389
>TC78186 weakly similar to GP|3128176|gb|AAC16080.1| unknown protein
{Arabidopsis thaliana}, partial (32%)
Length = 766
Score = 51.6 bits (122), Expect = 2e-07
Identities = 25/79 (31%), Positives = 42/79 (52%), Gaps = 2/79 (2%)
Frame = +2
Query: 34 DCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLKSVLKTAPSCLCEAFKSSS--QFGVVL 91
DC++ ++ + CL +V + P CC+ LK VL C+C K S+ + G +
Sbjct: 92 DCADKLVTLASCLPYVGGSANT--PTIDCCTNLKQVLNNTKKCICILIKDSNDPKLGFPM 265
Query: 92 NVTKALALPSACKVSASNS 110
N T A+ LP+AC + ++ S
Sbjct: 266 NATLAVQLPNACHIPSNIS 322
>TC81134 weakly similar to GP|14334664|gb|AAK59510.1 unknown protein
{Arabidopsis thaliana}, partial (42%)
Length = 900
Score = 50.1 bits (118), Expect = 5e-07
Identities = 27/96 (28%), Positives = 48/96 (49%), Gaps = 1/96 (1%)
Frame = +3
Query: 11 VVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDS-TVTKPQGQCCSGLKSV 69
+ + AV A +A + + S + ++ CLSF+TN S T P CC+ +K++
Sbjct: 84 LTVVLAVVTAMAAPAYAQITTPCNMSMISSTISPCLSFLTNSSGNGTSPTADCCNAIKTL 263
Query: 70 LKTAPSCLCEAFKSSSQFGVVLNVTKALALPSACKV 105
+ C+C + F + +N T A++LP AC +
Sbjct: 264 TSGSKDCMCLIATGNVPFALPINRTLAISLPRACNL 371
>TC79217 weakly similar to GP|21553541|gb|AAM62634.1 lipid transfer protein
putative {Arabidopsis thaliana}, partial (43%)
Length = 992
Score = 49.7 bits (117), Expect = 7e-07
Identities = 30/113 (26%), Positives = 52/113 (45%), Gaps = 5/113 (4%)
Frame = +1
Query: 7 LILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGL 66
+ LCV+A+ N A ++ C +++ K+ CL F T + P+ +CC
Sbjct: 205 MCLCVLALII------GGCNGAEDLASKCGSVVQKVIPCLDFATGKAPT--PKKECCDAA 360
Query: 67 KSVLKTAPSCLCEAFK-----SSSQFGVVLNVTKALALPSACKVSASNSVTNC 114
S+ T P CLC + S + + K L LP+ C V+ +N +++C
Sbjct: 361 NSIKATDPECLCYIIQQTHKGSPESKSMGIQEDKLLQLPTVCHVNGAN-ISDC 516
>TC77556 weakly similar to SP|Q43766|NLT3_HORVU Nonspecific lipid-transfer
protein 3 precursor (CW20) (CW-20) (CW-19). [Barley]
{Hordeum vulgare}, partial (28%)
Length = 662
Score = 47.4 bits (111), Expect = 3e-06
Identities = 37/113 (32%), Positives = 54/113 (47%), Gaps = 7/113 (6%)
Frame = +2
Query: 1 MASKVSLI--LCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKP 58
MAS + +I C+ IC V A++ S C + +T CL +VTN T++ P
Sbjct: 86 MASSMLVIKVTCLAMICLVLCIPLANA------SLTCDEVKQNLTPCLPYVTNPHTLSPP 247
Query: 59 QGQCCSGLKSV-----LKTAPSCLCEAFKSSSQFGVVLNVTKALALPSACKVS 106
QCC+G+K+V +K +C KS LN T A LPS C ++
Sbjct: 248 D-QCCNGVKTVNDNAQIKPDRQDVCRCLKSLLTGVPGLNGTVASTLPSDCGIN 403
>TC76731 similar to GP|13937151|gb|AAK50069.1 AT5g57180/MUL3_13 {Arabidopsis
thaliana}, partial (10%)
Length = 1235
Score = 45.1 bits (105), Expect = 2e-05
Identities = 26/90 (28%), Positives = 40/90 (43%), Gaps = 1/90 (1%)
Frame = +3
Query: 26 NNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLKSVLKTAPSCLCEAFKSSS 85
N A S D + + C ++ + TKP CC +K ++T +CLC F +
Sbjct: 555 NTTEAQSTDIPSCATNLIPCADYLNS----TKPPSSCCDPIKKTVETELTCLCNLFYAPG 722
Query: 86 QFGVV-LNVTKALALPSACKVSASNSVTNC 114
+N T+ALAL C V + +T C
Sbjct: 723 LLATFNINTTQALALSRNCGV--TTDLTTC 806
>TC88967 weakly similar to GP|18477856|emb|CAC86258. lipid transfer protein
{Fragaria x ananassa}, partial (48%)
Length = 743
Score = 43.9 bits (102), Expect = 4e-05
Identities = 29/119 (24%), Positives = 50/119 (41%), Gaps = 10/119 (8%)
Frame = +1
Query: 8 ILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGLK 67
++C+ +C V F A A C ++ + C+S++ N T P QCC+G++
Sbjct: 73 LVCLAIVCLVTFGPKAE-----AAVTSCGPVVTSLYPCVSYIMNGGN-TVPAAQCCNGIR 234
Query: 68 SVLKTAP---------SCLCEAFKSSSQFGVVLNVTKALALPSACKVSASNSVT-NCGC 116
++ A +C+ A S LN+ A LP C V+ ++ N C
Sbjct: 235 NLNTMAQTTNDRRAVCTCIKNAVSQSGFSYTNLNLNLAAGLPRKCGVNIPYQISPNTDC 411
>TC86318 similar to GP|15451110|gb|AAK96826.1 Unknown protein {Arabidopsis
thaliana}, partial (79%)
Length = 618
Score = 40.0 bits (92), Expect = 5e-04
Identities = 26/111 (23%), Positives = 45/111 (40%)
Frame = +2
Query: 5 VSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCS 64
V + L ++ ++ + P SA S ++ CL V N P CC+
Sbjct: 89 VVMFLLASSVFILESEAAGECGRTPIGSAAAS-----LSPCLGAVRNVRAKVPPV--CCA 247
Query: 65 GLKSVLKTAPSCLCEAFKSSSQFGVVLNVTKALALPSACKVSASNSVTNCG 115
+ ++L+T+P CLC S +N A+ +P C + + CG
Sbjct: 248 RVGALLRTSPRCLCSVLLSPLAKQAKINPAIAITVPKRCNIRNRPAGKKCG 400
>TC86319 similar to GP|15451110|gb|AAK96826.1 Unknown protein {Arabidopsis
thaliana}, partial (79%)
Length = 760
Score = 39.7 bits (91), Expect = 7e-04
Identities = 26/111 (23%), Positives = 44/111 (39%)
Frame = +3
Query: 5 VSLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCS 64
V + L ++ ++ P SA S ++ CL V N P CC+
Sbjct: 78 VVMFLLASSVFILESEAGGECGRTPIGSAAAS-----LSPCLGAVRNVRAKVPPV--CCA 236
Query: 65 GLKSVLKTAPSCLCEAFKSSSQFGVVLNVTKALALPSACKVSASNSVTNCG 115
+ ++L+T+P CLC S +N A+ +P C + + CG
Sbjct: 237 RVGALLRTSPRCLCSVLLSPLAKQAKINPAIAITVPKRCNIRNRPAGKKCG 389
>TC77633 similar to GP|6648215|gb|AAF21213.1| unknown protein {Arabidopsis
thaliana}, partial (85%)
Length = 1300
Score = 34.3 bits (77), Expect = 0.029
Identities = 23/70 (32%), Positives = 37/70 (52%), Gaps = 5/70 (7%)
Frame = -1
Query: 6 SLILCVVAICAVDFAHSASSNNAPAPSADCSNLILKMTDCLSFVT-NDSTVTKPQGQ--- 61
+L+ V+ ICA+ + A+S+ AP+P N LK + ++F DST+ G+
Sbjct: 892 TLLASVLRICAMALSLPATSSTAPSP-----NGTLKCFESINFTALRDSTIQPGSGE*RS 728
Query: 62 -CCSGLKSVL 70
CC G K+ L
Sbjct: 727 SCCGGSKTPL 698
>TC81257 similar to GP|9279781|dbj|BAB01433.1 glucosyltransferase-like
protein {Arabidopsis thaliana}, partial (24%)
Length = 783
Score = 33.5 bits (75), Expect = 0.049
Identities = 21/63 (33%), Positives = 32/63 (50%)
Frame = +2
Query: 123 LIIPHLGFLRFIFFCIKFGIFNLLLMKNYIPLWISCYVSNEVFFFLFCFAVSETPAAAPG 182
LI+P F F FFCI F + + ++ +PLW+ CYV + F + +P + P
Sbjct: 23 LILP---FYSFTFFCIILP-FPMFIPESELPLWVICYVPIVMSF----LNILPSPKSVPF 178
Query: 183 LFP 185
L P
Sbjct: 179 LVP 187
>TC83386 similar to GP|14334664|gb|AAK59510.1 unknown protein {Arabidopsis
thaliana}, partial (44%)
Length = 671
Score = 33.5 bits (75), Expect = 0.049
Identities = 15/40 (37%), Positives = 24/40 (59%)
Frame = +3
Query: 66 LKSVLKTAPSCLCEAFKSSSQFGVVLNVTKALALPSACKV 105
LKS+ + +CLC +S F + +N T A++LP AC +
Sbjct: 261 LKSLTSSGMNCLCLLVTASVPFKIPINRTLAISLPRACNM 380
>TC76832 weakly similar to GP|21592534|gb|AAM64483.1 unknown {Arabidopsis
thaliana}, partial (71%)
Length = 601
Score = 32.7 bits (73), Expect = 0.084
Identities = 27/109 (24%), Positives = 40/109 (35%), Gaps = 1/109 (0%)
Frame = +1
Query: 8 ILCVVAICA-VDFAHSASSNNAPAPSADCSNLILKMTDCLSFVTNDSTVTKPQGQCCSGL 66
+LC+V I V H S N K+ C S +++ CC+ +
Sbjct: 73 LLCIVGIAVLVAGTHRVESAGECGRGTTPDNEAFKLIPCASAAKDENASVSQS--CCAQV 246
Query: 67 KSVLKTAPSCLCEAFKSSSQFGVVLNVTKALALPSACKVSASNSVTNCG 115
K L PSCLC S+ N A+ +P C ++ CG
Sbjct: 247 KK-LGQNPSCLCAVMLSNVAKMSGANPQIAVTIPKRCNLADRPVGYKCG 390
>AJ548147 similar to GP|15010698|gb| AT3g22600/F16J14_17 {Arabidopsis
thaliana}, partial (26%)
Length = 390
Score = 32.7 bits (73), Expect = 0.084
Identities = 15/33 (45%), Positives = 19/33 (57%), Gaps = 1/33 (3%)
Frame = -1
Query: 74 PSCLCEAFKSS-SQFGVVLNVTKALALPSACKV 105
P CLC+ S G+ +N T+ALA P AC V
Sbjct: 384 PQCLCQVLDGGGSSLGIKVNQTQALAWPCACNV 286
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.328 0.138 0.439
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,190,582
Number of Sequences: 36976
Number of extensions: 156059
Number of successful extensions: 1610
Number of sequences better than 10.0: 109
Number of HSP's better than 10.0 without gapping: 1588
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1599
length of query: 185
length of database: 9,014,727
effective HSP length: 90
effective length of query: 95
effective length of database: 5,686,887
effective search space: 540254265
effective search space used: 540254265
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0314.16