BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0312.5
(204 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC84423 similar to PIR|F84730|F84730 probable myosin heavy chain... 40 8e-04
TC91265 similar to GP|23297205|gb|AAN12917.1 At1g15130/F9L1_7 {A... 35 0.015
TC86622 weakly similar to GP|20197623|gb|AAM15156.1 putative myo... 35 0.026
TC81883 weakly similar to GP|7110148|gb|AAF36810.1| DNA repair-r... 33 0.098
TC87237 similar to GP|160409|gb|AAA29651.1|| mature-parasite-inf... 32 0.13
TC76532 weakly similar to SP|Q9BBN6|YCF1_LOTJA Hypothetical 214.... 32 0.17
TC81279 similar to PIR|T47949|T47949 hypothetical protein F2A19.... 32 0.22
BG646701 32 0.22
TC77763 similar to GP|15215674|gb|AAK91382.1 AT4g27500/F27G19_10... 31 0.29
TC82663 similar to PIR|T51505|T51505 hypothetical protein F5E19_... 30 0.64
TC78255 homologue to PIR|S47243|S47243 starch phosphorylase (EC ... 29 1.1
TC77503 weakly similar to GP|16930417|gb|AAL31894.1 At2g24420/T2... 28 1.9
TC83625 similar to GP|8096269|dbj|BAA95789.1 KED {Nicotiana taba... 28 2.4
TC87794 similar to GP|13775107|gb|AAK39130.1 bZIP transcription ... 28 2.4
TC79948 similar to PIR|A84830|A84830 hypothetical protein At2g40... 28 2.4
TC87675 weakly similar to PIR|T05063|T05063 hypothetical protein... 28 2.4
TC89336 weakly similar to GP|21554135|gb|AAM63215.1 unknown {Ara... 27 4.1
TC87387 homologue to PIR|T47775|T47775 hypothetical protein F24I... 27 4.1
TC78289 similar to GP|15451044|gb|AAK96793.1 Unknown protein {Ar... 27 4.1
>TC84423 similar to PIR|F84730|F84730 probable myosin heavy chain [imported]
- Arabidopsis thaliana, partial (10%)
Length = 787
Score = 39.7 bits (91), Expect = 8e-04
Identities = 46/166 (27%), Positives = 73/166 (43%), Gaps = 14/166 (8%)
Frame = +2
Query: 53 KEITKLKATMKLLSNSNK---TNEKKAADLASELNKMKKDFENSLKTKDE---EIVNLNT 106
+E+TKL A K L + + N K +L S+L + K + S D + ++ N+
Sbjct: 17 EELTKLNAEKKGLEETVEDLTVNAKHFKELCSDLEEKLKLSDESFSKTDSLLSQALSNNS 196
Query: 107 EAVQMKSSLESLQAEYSKLSVESSSLKISVLDHLEAGFAKAKEQISFLNPELAINFKGSD 166
E Q SLE L E ++ +S + + H+EA A A+E S L EL F ++
Sbjct: 197 ELEQKVKSLEDLHNESGAVAATASQRSLELEGHIEATNAAAEEAKSQLR-ELETRFIAAE 373
Query: 167 PYARIVDGKL----VSPNSAE----EEEEKNEEDNGNNKEGEGENH 204
++ +L + N AE E EK + KE E E +
Sbjct: 374 QKNVELEQQLNLVQLKANDAERDVTEFSEKISHLDAKLKEAEEEKN 511
>TC91265 similar to GP|23297205|gb|AAN12917.1 At1g15130/F9L1_7 {Arabidopsis
thaliana}, partial (31%)
Length = 828
Score = 35.4 bits (80), Expect = 0.015
Identities = 35/142 (24%), Positives = 65/142 (45%)
Frame = +1
Query: 49 EEKDKEITKLKATMKLLSNSNKTNEKKAADLASELNKMKKDFENSLKTKDEEIVNLNTEA 108
EE +EI +LK+ + +L+ + K + AA L +NK++ + +L+ +E +
Sbjct: 250 EEIAEEIARLKSAVSVLTEAKKNSRGAAAQLLDAINKLEVNINRNLERAVKENDRVYLMR 429
Query: 109 VQMKSSLESLQAEYSKLSVESSSLKISVLDHLEAGFAKAKEQISFLNPELAINFKGSDPY 168
V SSL L A ++ + VLD A ++ + L P+ + K Y
Sbjct: 430 VPPASSLSPLPA----FAMVKPMVMSEVLD------ASKEKMFASLVPDNSA--KALSRY 573
Query: 169 ARIVDGKLVSPNSAEEEEEKNE 190
+VDG + AE+ ++ +E
Sbjct: 574 TEMVDG--IIRTQAEKLQQASE 633
>TC86622 weakly similar to GP|20197623|gb|AAM15156.1 putative myosin heavy
chain {Arabidopsis thaliana}, partial (17%)
Length = 2546
Score = 34.7 bits (78), Expect = 0.026
Identities = 27/94 (28%), Positives = 46/94 (48%)
Frame = +3
Query: 30 KMIDLESNVFDGTDNAKQLEEKDKEITKLKATMKLLSNSNKTNEKKAADLASELNKMKKD 89
K+ +LE + D + ++ + +L+A +K NS + E K ++ S L K +
Sbjct: 1695 KVAELEKELHLAQDTIANQKGEESQKLELEAALK---NSVEELETKKNEI-SLLQKQVIE 1862
Query: 90 FENSLKTKDEEIVNLNTEAVQMKSSLESLQAEYS 123
FE L+ DE+I EAV K +LE ++S
Sbjct: 1863 FEQKLQQADEKISVKGEEAVDKKDALEVKSRDFS 1964
>TC81883 weakly similar to GP|7110148|gb|AAF36810.1| DNA
repair-recombination protein {Arabidopsis thaliana},
partial (10%)
Length = 1057
Score = 32.7 bits (73), Expect = 0.098
Identities = 33/125 (26%), Positives = 54/125 (42%), Gaps = 14/125 (11%)
Frame = +1
Query: 23 LEITAISK-MIDLESNVFDGTDNAKQLEEKDKEITKLKATMKLLSN--SNKTNEKKAAD- 78
LEI + K + DLES + G + LEE E+ L+ T L +N +E++ D
Sbjct: 475 LEIQTLQKKVEDLESELGCGGPEVRTLEEIQLELVALQGTKDNLHTELTNLMDEQRNMDT 654
Query: 79 ----LASELNKMKKDFENS------LKTKDEEIVNLNTEAVQMKSSLESLQAEYSKLSVE 128
+ S L + D N+ +K +EE+ L E Q+ +SL + LS
Sbjct: 655 DISGIQSRLQNARADKMNAAMKLQDVKRLEEELERLTEEMTQVDLDEKSLTEAFGPLSKR 834
Query: 129 SSSLK 133
++
Sbjct: 835 KDKIR 849
>TC87237 similar to GP|160409|gb|AAA29651.1|| mature-parasite-infected
erythrocyte surface antigen {Plasmodium falciparum},
partial (2%)
Length = 2007
Score = 32.3 bits (72), Expect = 0.13
Identities = 26/85 (30%), Positives = 41/85 (47%), Gaps = 2/85 (2%)
Frame = +1
Query: 118 LQAEYSKLSVESSSLKISVLDHLEAGFAKAKEQISFL-NPELAINFKGSDPYARIVDG-K 175
L+ E SK + ESS K + + ++ E+ S L N E K I DG K
Sbjct: 16 LENEESKETGESSEEKSHLENEESKETGESSEEKSHLENEENKDEEKSKQENEEIKDGEK 195
Query: 176 LVSPNSAEEEEEKNEEDNGNNKEGE 200
+ N ++EEK++++N NK+ E
Sbjct: 196 IQQENEENKDEEKSQQENEENKDEE 270
>TC76532 weakly similar to SP|Q9BBN6|YCF1_LOTJA Hypothetical 214.8 kDa protein
ycf1. {Lotus japonicus}, partial (21%)
Length = 4509
Score = 32.0 bits (71), Expect = 0.17
Identities = 23/72 (31%), Positives = 37/72 (50%), Gaps = 7/72 (9%)
Frame = +1
Query: 58 LKATMKLLSNSNKTNEKKAADLASELNKMKKDFEN-------SLKTKDEEIVNLNTEAVQ 110
L++T K+L+ +KK ++ +M++ FEN S+ K E I N+N++
Sbjct: 2377 LESTDKILNVKKPIYDKK-----KKMEEMEERFENLSVSRLISILEKSENITNMNSQNSW 2541
Query: 111 MKSSLESLQAEY 122
SSL SL EY
Sbjct: 2542 DVSSLSSLSQEY 2577
>TC81279 similar to PIR|T47949|T47949 hypothetical protein F2A19.170 -
Arabidopsis thaliana, partial (31%)
Length = 1205
Score = 31.6 bits (70), Expect = 0.22
Identities = 25/129 (19%), Positives = 58/129 (44%)
Frame = +2
Query: 47 QLEEKDKEITKLKATMKLLSNSNKTNEKKAADLASELNKMKKDFENSLKTKDEEIVNLNT 106
Q+ ++ + + + + L ++N + + ++ +LNK + +++ K+ E++NL T
Sbjct: 581 QISHLERALEQATSDQEKLKSANNSEILTSREVIDDLNKKLTNCISTIDAKNIELINLQT 760
Query: 107 EAVQMKSSLESLQAEYSKLSVESSSLKISVLDHLEAGFAKAKEQISFLNPELAINFKGSD 166
Q + +E+ +HLE A+A+E+ + L+ L K +D
Sbjct: 761 ALGQYYAEIEA-------------------KEHLEEELARAREETANLSQLL----KDAD 871
Query: 167 PYARIVDGK 175
I+ G+
Sbjct: 872 SRVDILSGE 898
>BG646701
Length = 707
Score = 31.6 bits (70), Expect = 0.22
Identities = 23/74 (31%), Positives = 37/74 (49%), Gaps = 12/74 (16%)
Frame = -1
Query: 63 KLLSNSNKTNEKKAAD-------LASELNKMKKDFENSLKTKDEEIVNLNTEA-----VQ 110
K L + T E + +D + KMK DF+ + TK++E V+L T A +
Sbjct: 455 KKLGSKEVTQEGETSDGDSVQSKIEETEEKMKLDFDLNSSTKEKEEVSLLTGALFSRWIN 276
Query: 111 MKSSLESLQAEYSK 124
+SSLE++ E S+
Sbjct: 275 FQSSLENIHTELSR 234
>TC77763 similar to GP|15215674|gb|AAK91382.1 AT4g27500/F27G19_100
{Arabidopsis thaliana}, partial (38%)
Length = 2297
Score = 31.2 bits (69), Expect = 0.29
Identities = 34/127 (26%), Positives = 52/127 (40%), Gaps = 1/127 (0%)
Frame = +1
Query: 5 IPKKHISRI-ICQALITKGLEITAISKMIDLESNVFDGTDNAKQLEEKDKEITKLKATMK 63
I KK+ +R I +AL K E + + I + D +EK KEI L+ +
Sbjct: 520 ISKKNQARFQITEALKAKRSEKSELISQIKILRE--DNRQYQSLFDEKMKEIEPLQQALG 693
Query: 64 LLSNSNKTNEKKAADLASELNKMKKDFENSLKTKDEEIVNLNTEAVQMKSSLESLQAEYS 123
L NSN ELNK F N L+ + + TE Q+ ++ L+
Sbjct: 694 KLRNSNNAGRGGICQSEEELNK----FINGLQYRIQHESIPLTEEKQILREIKQLEGTRE 861
Query: 124 KLSVESS 130
K+ S+
Sbjct: 862 KVIANSA 882
>TC82663 similar to PIR|T51505|T51505 hypothetical protein F5E19_70 -
Arabidopsis thaliana, partial (20%)
Length = 914
Score = 30.0 bits (66), Expect = 0.64
Identities = 32/154 (20%), Positives = 63/154 (40%), Gaps = 15/154 (9%)
Frame = +3
Query: 16 QALITKGLEITAISKMIDLESNVFDGTDNAKQLEEKDK---------------EITKLKA 60
QAL + L + + +++ ++ + + DN+++ EEK K E + K
Sbjct: 282 QALNNEQLAASHVQTLLEEKNKLINELDNSREEEEKSKKAMESLASALHEVSAESREAKE 461
Query: 61 TMKLLSNSNKTNEKKAADLASELNKMKKDFENSLKTKDEEIVNLNTEAVQMKSSLESLQA 120
++ E + DL L + +E+ L EI L K+ E+ +A
Sbjct: 462 NFLSTQAERESYENQIEDLKLVLKGTNEKYESMLDEAQHEIDVLIDSIENSKNVFENSKA 641
Query: 121 EYSKLSVESSSLKISVLDHLEAGFAKAKEQISFL 154
E+ + + +S L E A A+++I+ L
Sbjct: 642 EWDQRELH----LVSSLKKTEEENAAAEKEINRL 731
>TC78255 homologue to PIR|S47243|S47243 starch phosphorylase (EC 2.4.1.1)
isoform L precursor chloroplast - fava bean, partial
(47%)
Length = 1814
Score = 29.3 bits (64), Expect = 1.1
Identities = 37/184 (20%), Positives = 77/184 (41%), Gaps = 13/184 (7%)
Frame = +2
Query: 28 ISKMIDLESNVFDGTDNAKQLEEKDKEITKLKATMKLLSN----------SNKTNEKKAA 77
+ + I + G+ AKQ + KD+EI + + LS+ S K + +
Sbjct: 275 LRRRISFQVKCLFGSSEAKQ-KLKDREIQEQQEATTSLSSFAPDASSIVSSIKYHAEFTP 451
Query: 78 DLASELNKMKKDFENSLKT-KDEEIVNLNTEAVQMKSSLESLQAEYSKLSVESSSLKISV 136
+ E ++ + F + +T +D I+N N L QA Y + ++
Sbjct: 452 LFSPEKFELPQAFIATAQTVRDALIINWNA-TYDYYEKLNVKQAYYLSMEFLQGRALLNA 628
Query: 137 LDHLE--AGFAKAKEQISFLNPELAINFKGSDPYARIVDGKLVSPNSAEEEEEKNEEDNG 194
+ +LE +A+A Q+ + + + + +D + + +S + E+ E+D+G
Sbjct: 629 IGNLELTGPYAEALSQLGYKLENVELPAEFADVLVKSKEADDISSEEVKISGEEEEDDDG 808
Query: 195 NNKE 198
N+ E
Sbjct: 809 NDDE 820
>TC77503 weakly similar to GP|16930417|gb|AAL31894.1 At2g24420/T28I24.15
{Arabidopsis thaliana}, partial (54%)
Length = 1787
Score = 28.5 bits (62), Expect = 1.9
Identities = 39/170 (22%), Positives = 75/170 (43%), Gaps = 7/170 (4%)
Frame = +2
Query: 38 VFDGTDNAKQLEEKDKEITKLKATMKLLSNSNKTNEKKAADLASELNKMKKDFENSLKTK 97
+F + + +D + + LK + L++ ++ E + ++ EL K K
Sbjct: 155 IFSFVTSEADVSIEDSDSSALKIQLDQLNSKIQSLESQISEKTQELKK-----------K 301
Query: 98 DEEIVNLNTEAVQMKSSLESLQAEYSKLSVESSSLKISVLDHLEAGFAKAKE---QISFL 154
D+ I SS++SLQ E + L + S + + + +A+A E Q+ L
Sbjct: 302 DQVIAEKEKLFQDKLSSIQSLQNEVASLQKKGS---LDAEEQVGKAYARAGELQKQVDKL 472
Query: 155 NPEL-AINFKGSDPYARI--VDGKLVSPNSAEEEEEK-NEEDNGNNKEGE 200
EL A N + + +R+ ++ K+ NS E+ +K NEE ++ E
Sbjct: 473 KSELEAQNSEKVNWESRVAKLEKKIHDLNSKLEDVQKINEEQKKQIRKTE 622
>TC83625 similar to GP|8096269|dbj|BAA95789.1 KED {Nicotiana tabacum},
partial (9%)
Length = 908
Score = 28.1 bits (61), Expect = 2.4
Identities = 28/126 (22%), Positives = 54/126 (42%), Gaps = 1/126 (0%)
Frame = +2
Query: 70 KTNEKKAADLASELNKMKKDFENSLKTKDEEIVNLNTEAVQMKSSLESLQAEYSKLSVES 129
K ++K A D+ + +++KD E KD+E + + K+ ++ + + K +
Sbjct: 482 KIDDKSAGDVKEDKVEIEKDLEIKSVEKDDEKKEKKDKEKKDKTDVDEGKDKKDKEKKKK 661
Query: 130 SSLKISVLDHLEAGFAKA-KEQISFLNPELAINFKGSDPYARIVDGKLVSPNSAEEEEEK 188
+ +V E G K KE+ E K D + S E++++K
Sbjct: 662 EKKEENVKGEEEDGDEKKDKEKKKKEKKEKGKEDKDKDGEEK------KSKKDKEKKKDK 823
Query: 189 NEEDNG 194
NE+D+G
Sbjct: 824 NEDDDG 841
>TC87794 similar to GP|13775107|gb|AAK39130.1 bZIP transcription factor 2
{Phaseolus vulgaris}, partial (50%)
Length = 1264
Score = 28.1 bits (61), Expect = 2.4
Identities = 15/64 (23%), Positives = 35/64 (54%)
Frame = +2
Query: 70 KTNEKKAADLASELNKMKKDFENSLKTKDEEIVNLNTEAVQMKSSLESLQAEYSKLSVES 129
+ K++ ++ ++++K E + E+ LN E +++ L +++EY K+ E+
Sbjct: 392 RQRRKQSNRESARRSRLRKQAECDELAQRAEV--LNQENASLRAELSPIKSEYEKIPSEN 565
Query: 130 SSLK 133
+SLK
Sbjct: 566 ASLK 577
>TC79948 similar to PIR|A84830|A84830 hypothetical protein At2g40480
[imported] - Arabidopsis thaliana, partial (28%)
Length = 1128
Score = 28.1 bits (61), Expect = 2.4
Identities = 34/151 (22%), Positives = 60/151 (39%), Gaps = 1/151 (0%)
Frame = +3
Query: 1 MQSLIPKKHISRIICQALITKGLEITAISKMIDLESNVFDGTDNAKQLEEKDKEITKLKA 60
++ L K I + Q L + L+++A DL SN GT K++ ++
Sbjct: 318 LEELGATKRIVEDLKQQLQKEALKLSATP---DLNSNEQVGTPVIKEMNKETN------- 467
Query: 61 TMKLLSNSNKTNEKKAADLASELNKMKKDFE-NSLKTKDEEIVNLNTEAVQMKSSLESLQ 119
N+ N+++ + + S + D LK + N E ++SS+ESL
Sbjct: 468 -----GNNVNNNQEQTSHIPSSCSMSSPDMILMDLKQAKVNLGNTMNELEAIQSSVESLN 632
Query: 120 AEYSKLSVESSSLKISVLDHLEAGFAKAKEQ 150
+ K V + + A A+ KEQ
Sbjct: 633 EKMKKEKVFLEKTREKLASKFAAVSAQEKEQ 725
>TC87675 weakly similar to PIR|T05063|T05063 hypothetical protein M3E9.160 -
Arabidopsis thaliana, partial (41%)
Length = 1191
Score = 28.1 bits (61), Expect = 2.4
Identities = 22/91 (24%), Positives = 39/91 (42%)
Frame = +3
Query: 46 KQLEEKDKEITKLKATMKLLSNSNKTNEKKAADLASELNKMKKDFENSLKTKDEEIVNLN 105
K+++ E+ A ++ L+ S+ E +A DL L + E + L
Sbjct: 573 KEMKYGHSELMNTGAQLQRLAKSSYKAEARATDLIDRLRDIPS----------REALTLR 722
Query: 106 TEAVQMKSSLESLQAEYSKLSVESSSLKISV 136
E + SSL+ ++ K ++ S L ISV
Sbjct: 723 AEVASLASSLKRQRSVLDKRIMKISELGISV 815
>TC89336 weakly similar to GP|21554135|gb|AAM63215.1 unknown {Arabidopsis
thaliana}, partial (7%)
Length = 1241
Score = 27.3 bits (59), Expect = 4.1
Identities = 25/108 (23%), Positives = 49/108 (45%), Gaps = 14/108 (12%)
Frame = +1
Query: 33 DLESNVFDGTDNAKQLEEKDKEITKL-------KATMKLLSN------SNKTN-EKKAAD 78
+LESN+ + QLE + +TKL + ++ L + +N+TN E +
Sbjct: 775 ELESNLAETRQLQDQLEVAQENVTKLEWQLDSGRKQIRELEDRITWFKTNETNLEVEVQK 954
Query: 79 LASELNKMKKDFENSLKTKDEEIVNLNTEAVQMKSSLESLQAEYSKLS 126
L E++ ++ F +I +L+ Q+ S+LE ++ L+
Sbjct: 955 LKDEMHDVQAQFSFEKDQLHSDIASLSEIKTQLTSTLEEWESRSHSLA 1098
>TC87387 homologue to PIR|T47775|T47775 hypothetical protein F24I3.230 -
Arabidopsis thaliana, partial (14%)
Length = 1074
Score = 27.3 bits (59), Expect = 4.1
Identities = 20/65 (30%), Positives = 25/65 (37%), Gaps = 8/65 (12%)
Frame = +3
Query: 46 KQLEEKDKEITKLKATMKLLSNSNKTNEKKAADLASELN--------KMKKDFENSLKTK 97
K+ E D K+ + + K KK D A E+ K KKD EN
Sbjct: 261 KKHESADSPAPAKKSKVAEVDGEEKVKTKKVDDAAVEVEDDKKEKKKKKKKDKENGAAAS 440
Query: 98 DEEIV 102
DEE V
Sbjct: 441 DEEKV 455
>TC78289 similar to GP|15451044|gb|AAK96793.1 Unknown protein {Arabidopsis
thaliana}, partial (15%)
Length = 1020
Score = 27.3 bits (59), Expect = 4.1
Identities = 18/76 (23%), Positives = 36/76 (46%), Gaps = 14/76 (18%)
Frame = +1
Query: 46 KQLEEKDKEITKLK-------ATMKLLSNSNK-------TNEKKAADLASELNKMKKDFE 91
+++EEKD+E+ K+K A LL NK + A+ A + ++ D +
Sbjct: 664 RKMEEKDRELHKIKLDNEAAWAKQDLLREQNKELASFRRERDHSEAERAQHIQQI-HDLQ 840
Query: 92 NSLKTKDEEIVNLNTE 107
++ KD +++ L +
Sbjct: 841 EHIQEKDRQLIELQEQ 888
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.303 0.123 0.311
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,208,095
Number of Sequences: 36976
Number of extensions: 40848
Number of successful extensions: 297
Number of sequences better than 10.0: 49
Number of HSP's better than 10.0 without gapping: 276
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 289
length of query: 204
length of database: 9,014,727
effective HSP length: 92
effective length of query: 112
effective length of database: 5,612,935
effective search space: 628648720
effective search space used: 628648720
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 43 (21.8 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0312.5