BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0310b.11
(371 letters)
Database: MTGI
36,976 sequences; 27,044,181 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC92184 similar to GP|20466394|gb|AAM20514.1 unknown protein {Ar... 299 1e-81
AW256667 weakly similar to GP|2252851|gb| A_TM018A10.3 gene prod... 138 4e-33
TC82682 similar to GP|20466394|gb|AAM20514.1 unknown protein {Ar... 115 4e-26
CA858644 weakly similar to PIR|E84670|E84 hypothetical protein A... 110 9e-25
TC84482 similar to PIR|D86157|D86157 hypothetical protein AAF028... 38 0.007
AW776630 weakly similar to PIR|S18883|S188 ribonucleoprotein pre... 30 1.9
TC91399 weakly similar to PIR|S53494|S53494 RNA-binding protein ... 30 1.9
TC90928 homologue to GP|21593435|gb|AAM65402.1 putative plant ad... 28 4.3
TC86266 similar to PIR|T48498|T48498 hypothetical protein T28J14... 28 7.4
BG644699 similar to PIR|T07863|T078 probable polyprotein - pinea... 27 9.7
TC93404 27 9.7
>TC92184 similar to GP|20466394|gb|AAM20514.1 unknown protein {Arabidopsis
thaliana}, partial (26%)
Length = 1024
Score = 299 bits (765), Expect = 1e-81
Identities = 139/160 (86%), Positives = 151/160 (93%)
Frame = +2
Query: 212 ITYCYIIVSGYQTGKFIDTAVSRFLLIALGAAVSLGVNVCLYPIWAGEDLHDLVAKNFMG 271
ITYCY+IVSGY+TG F+ TA +RFLLIALGAAVS+GVNVC+YPIWAGEDLH LVAKNF G
Sbjct: 2 ITYCYVIVSGYRTGDFLHTATNRFLLIALGAAVSVGVNVCIYPIWAGEDLHYLVAKNFTG 181
Query: 272 VAASLEGVVNNYLNCIEYERVPSKILTYQASDDVVYSGYRSALQSTSTEDALMGFAVWEP 331
VA SLEGVVNNYLNC+EY+RVPSKILTYQASDDVVYSGYRSA++STSTEDALM FA+WEP
Sbjct: 182 VATSLEGVVNNYLNCVEYDRVPSKILTYQASDDVVYSGYRSAVESTSTEDALMSFAIWEP 361
Query: 332 PHGRYKMLKYPWSNYVKVSGALRHCAFMVMAMHGCILSEI 371
PHGRYKM +YPW NYVKVSGALRHCAFMVMAMHGCILSEI
Sbjct: 362 PHGRYKMFRYPWKNYVKVSGALRHCAFMVMAMHGCILSEI 481
>AW256667 weakly similar to GP|2252851|gb| A_TM018A10.3 gene product
{Arabidopsis thaliana}, partial (29%)
Length = 631
Score = 138 bits (347), Expect = 4e-33
Identities = 65/160 (40%), Positives = 107/160 (66%), Gaps = 2/160 (1%)
Frame = +1
Query: 84 RAWEMGLSDPRKIVFAAKMGLALILISLLIFLKTPFEDLGRHSVWAILTVVTVFEFSIGA 143
+AWE+G++DPRK + K+G+AL +SL +LK ++ +GR+++WA++TV+ VFE++ GA
Sbjct: 151 KAWEIGVNDPRKFIHCLKVGIALTAVSLFYYLKPLYDGVGRNAMWAVMTVIVVFEYTAGA 330
Query: 144 TLSKGLNRGLGTLTAGGLAVGM-GVLSKLAGQWEEIIVMVSIFIVGFCATYAKLYPTMKA 202
T+ K +NR GT AG LA G+ V SK QWE +IV S+F++ T+++ PT+KA
Sbjct: 331 TIYKSVNRIFGTTLAGLLAFGVHWVASKAGDQWEPVIVGASLFLLASATTFSRFIPTIKA 510
Query: 203 -YEYGFRVFLITYCYIIVSGYQTGKFIDTAVSRFLLIALG 241
++YG +F++T+ + VSGY+ + + A R I +G
Sbjct: 511 RFDYGALIFILTFSLVSVSGYRVDELFNLAQQRISTIIIG 630
>TC82682 similar to GP|20466394|gb|AAM20514.1 unknown protein {Arabidopsis
thaliana}, partial (31%)
Length = 1243
Score = 115 bits (287), Expect = 4e-26
Identities = 52/64 (81%), Positives = 57/64 (88%), Gaps = 1/64 (1%)
Frame = +3
Query: 309 GYR-SALQSTSTEDALMGFAVWEPPHGRYKMLKYPWSNYVKVSGALRHCAFMVMAMHGCI 367
GYR ++STS ED+L+GFAVWEPPHG Y+ LKYPW NYVKVSGALRHCAFMVMAMHGCI
Sbjct: 18 GYRIRPVESTSNEDSLLGFAVWEPPHGHYRSLKYPWKNYVKVSGALRHCAFMVMAMHGCI 197
Query: 368 LSEI 371
LSEI
Sbjct: 198 LSEI 209
>CA858644 weakly similar to PIR|E84670|E84 hypothetical protein At2g27240
[imported] - Arabidopsis thaliana, partial (28%)
Length = 726
Score = 110 bits (275), Expect = 9e-25
Identities = 59/189 (31%), Positives = 101/189 (53%), Gaps = 2/189 (1%)
Frame = +1
Query: 177 EIIVMVSIFIVGFC-ATYAKLYPTMKA-YEYGFRVFLITYCYIIVSGYQTGKFIDTAVSR 234
+I +M FI A++ + +P +K+ Y+YG +F++T+ I VSG++ + + A R
Sbjct: 175 KINIMFYFFIFAAAIASFIRFFPKVKSRYDYGMLIFILTFSLISVSGFRDDEVLKMAHKR 354
Query: 235 FLLIALGAAVSLGVNVCLYPIWAGEDLHDLVAKNFMGVAASLEGVVNNYLNCIEYERVPS 294
I LG + + +++ + P+WAGE+LHD +A + LE V Y + E
Sbjct: 355 LSTIFLGGSACVMISIFVCPVWAGEELHDSIAIKLEILGDFLEAFVGEYFKTTKEEESKD 534
Query: 295 KILTYQASDDVVYSGYRSALQSTSTEDALMGFAVWEPPHGRYKMLKYPWSNYVKVSGALR 354
K GY+S L S S +++L FA WEP HG++K ++PW+ Y+K+ R
Sbjct: 535 K--------KSFLEGYKSILNSKSNDESLANFARWEPGHGKFK-FRHPWNQYLKIGALSR 687
Query: 355 HCAFMVMAM 363
CA+ + A+
Sbjct: 688 QCAYRMEAL 714
>TC84482 similar to PIR|D86157|D86157 hypothetical protein AAF02892.1
[imported] - Arabidopsis thaliana, partial (17%)
Length = 638
Score = 37.7 bits (86), Expect = 0.007
Identities = 21/56 (37%), Positives = 32/56 (56%), Gaps = 1/56 (1%)
Frame = +1
Query: 209 VFLITYCYIIVSGYQTGKFIDTAVS-RFLLIALGAAVSLGVNVCLYPIWAGEDLHD 263
+FLI YC++ +G+FI ++S FL+ LG V+L + L W+G LHD
Sbjct: 187 IFLIVYCFLPALSLFSGQFIVQSLSVTFLVFLLGITVTLCLLALLEIKWSGITLHD 354
>AW776630 weakly similar to PIR|S18883|S188 ribonucleoprotein precursor -
common tobacco (fragment), partial (31%)
Length = 719
Score = 29.6 bits (65), Expect = 1.9
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = -1
Query: 36 SQIGIPLPESDDDNDGPPGSKNCC 59
S++ + + +SD D+D SKNCC
Sbjct: 317 SKVSVSVSDSDSDSDSDSDSKNCC 246
>TC91399 weakly similar to PIR|S53494|S53494 RNA-binding protein cp33
precursor - Arabidopsis thaliana, partial (39%)
Length = 775
Score = 29.6 bits (65), Expect = 1.9
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = -2
Query: 36 SQIGIPLPESDDDNDGPPGSKNCC 59
S++ + + +SD D+D SKNCC
Sbjct: 207 SKVSVSVSDSDSDSDSDSDSKNCC 136
>TC90928 homologue to GP|21593435|gb|AAM65402.1 putative plant adhesion
molecule {Arabidopsis thaliana}, partial (66%)
Length = 1058
Score = 28.5 bits (62), Expect = 4.3
Identities = 18/42 (42%), Positives = 25/42 (58%)
Frame = +2
Query: 81 VAARAWEMGLSDPRKIVFAAKMGLALILISLLIFLKTPFEDL 122
+A R W++ L + KIVF K+GLAL+ +K PFE L
Sbjct: 476 LALRIWDVFLYEGVKIVF--KVGLALLKYCHDDLVKLPFEKL 595
>TC86266 similar to PIR|T48498|T48498 hypothetical protein T28J14.210 -
Arabidopsis thaliana, partial (53%)
Length = 1058
Score = 27.7 bits (60), Expect = 7.4
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = +1
Query: 231 AVSRFLLIALGAAVSLGVNVCLYPIWAGEDLHDLV 265
A FLLI LG +G+N C +P E L L+
Sbjct: 799 ATGGFLLILLGCGGVIGLNRCWHPFLMPEHLPSLI 903
>BG644699 similar to PIR|T07863|T078 probable polyprotein - pineapple
retrotransposon dea1 (fragment), partial (5%)
Length = 231
Score = 27.3 bits (59), Expect = 9.7
Identities = 13/38 (34%), Positives = 20/38 (52%)
Frame = +2
Query: 53 PGSKNCCRFCSYRALSDRLVGAWKTTKRVAARAWEMGL 90
P + CRF LS R +G ++ KR+ A+E+ L
Sbjct: 26 PTERGDCRFGKRGKLSLRYIGPFEVIKRIGEVAYELAL 139
>TC93404
Length = 948
Score = 27.3 bits (59), Expect = 9.7
Identities = 16/42 (38%), Positives = 23/42 (54%)
Frame = -2
Query: 1 MASSRVPIPKLGSFRYSFKERKEKLLSMKSSLIGYSQIGIPL 42
+ S RV GSF + + K KLLS+ S + YS+ G P+
Sbjct: 515 VCSQRVSPSVEGSFPILYAQEKSKLLSL*GSNLVYSRDGEPV 390
Database: MTGI
Posted date: Oct 22, 2004 3:39 PM
Number of letters in database: 27,044,181
Number of sequences in database: 36,976
Lambda K H
0.324 0.139 0.425
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,288,335
Number of Sequences: 36976
Number of extensions: 173794
Number of successful extensions: 1102
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 1092
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1097
length of query: 371
length of database: 9,014,727
effective HSP length: 98
effective length of query: 273
effective length of database: 5,391,079
effective search space: 1471764567
effective search space used: 1471764567
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0310b.11