Lotus
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0302a.10
         (243 letters)

Database: MTGI 
           36,976 sequences; 27,044,181 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC92943                                                               107  6e-25
BF643280                                                               42  2e-04
AL374857                                                               39  0.002
TC86982 similar to GP|10946337|gb|AAG24863.1 CONSTANS-like prote...    30  0.85
TC78447 similar to GP|7768151|emb|CAB90633.1 protein phpsphatase...    29  1.4
TC88552 similar to PIR|T51260|T51260 hypothetical protein T8M16_...    29  1.9
BQ144022 homologue to GP|22831315|dbj hypothetical protein~predi...    28  3.2
TC88202 homologue to GP|21615421|emb|CAD33930. arabinogalactan p...    28  4.2
TC84372 similar to GP|15146181|gb|AAK83574.1 AT3g14070/MAG2_2 {A...    27  5.5
CA990302 weakly similar to GP|10177640|db contains similarity to...    27  7.2
TC86794 similar to PIR|T06499|T06499 Rieske [2Fe-2S] iron-sulfur...    27  7.2
BG449891 similar to GP|17901818|gb 32 kDa ferritin subunit {Gall...    27  7.2
TC85947 similar to PIR|S52261|S52261 NADH dehydrogenase (ubiquin...    27  9.4
TC86147 GP|9758956|dbj|BAB09343.1 histone H2A {Arabidopsis thali...    27  9.4
TC92979 similar to GP|20127014|gb|AAM10934.1 putative bHLH trans...    27  9.4

>TC92943 
          Length = 672

 Score =  107 bits (267), Expect(2) = 6e-25
 Identities = 59/121 (48%), Positives = 72/121 (58%)
 Frame = +1

Query: 3   KAGRGKTTGTSVTKKKMKSATGKLPLCIPLDKMVAVGPGAPDFVTEISVLVKKKAPFNVK 62
           K GRG+T G SV KKK KSATGKL   IP DKMVAVG GA +FVTEIS++V K APFNVK
Sbjct: 310 KVGRGRTVGLSVAKKKKKSATGKLHDVIPTDKMVAVGSGAANFVTEISIVVLKNAPFNVK 489

Query: 63  GWKKVPDTAKDHILSQVFVNIII*FIFLFSFVLIQYLQYISCNCWVQDTFDIDQTEHNKE 122
            W+K+P   ++ I+S+V                              DTFDID T+HN++
Sbjct: 490 KWRKIPQETENKIVSKVL-----------------------------DTFDIDNTKHNRD 582

Query: 123 V 123
           V
Sbjct: 583 V 585



 Score = 23.5 bits (49), Expect(2) = 6e-25
 Identities = 9/13 (69%), Positives = 12/13 (92%)
 Frame = +2

Query: 124 ILNTAKRLYGSHR 136
           I++TAKRLY +HR
Sbjct: 587 IIDTAKRLYRNHR 625


>BF643280 
          Length = 582

 Score = 42.4 bits (98), Expect = 2e-04
 Identities = 29/95 (30%), Positives = 46/95 (47%)
 Frame = +2

Query: 124 ILNTAKRLYGSHRGKLHEHFKKYETNEMALEHKPDEISEEDWEHLVDKFSSPTYKEMSAR 183
           I+N A R Y     K   HF KY+     L+++P+ I E  ++ L+  +S    +E+S +
Sbjct: 272 IINDAWRWYKWLIKK--NHFTKYKNLCERLKNRPNSIPEAHFKKLMTYWSYEAIREISHQ 445

Query: 184 NKANKSKQVINHRCGRKSFQAVSYDARDPETQKEP 218
           N  N  KQ   H+ G  SF  +    R  +  +EP
Sbjct: 446 NAKNIDKQKWRHQAGPISFAVIRERLRATKDDREP 550


>AL374857 
          Length = 492

 Score = 38.9 bits (89), Expect = 0.002
 Identities = 22/91 (24%), Positives = 47/91 (51%), Gaps = 2/91 (2%)
 Frame = +1

Query: 155 HKPDEISEEDWEHLVDKFSSPTYKEMSARNKANKSKQVIN--HRCGRKSFQAVSYDARDP 212
           H P  +  E W  LVD +  P + + S  N+ N++ +  +  H  G  SF+       + 
Sbjct: 91  HGPKGLKPEVWNGLVDIWLKPEWTKKSDANRCNRAAKPDSALHTGGSISFRE-HKKRMEE 267

Query: 213 ETQKEPNYQDLWRMTHTNNNGEWVNEASREV 243
           E ++E +Y+D++   H + +G +++  S+++
Sbjct: 268 EMKQEVSYRDVYAHVHKSKDGGYISRRSKKL 360


>TC86982 similar to GP|10946337|gb|AAG24863.1 CONSTANS-like protein {Ipomoea
           nil}, partial (54%)
          Length = 1643

 Score = 30.0 bits (66), Expect = 0.85
 Identities = 14/36 (38%), Positives = 20/36 (54%), Gaps = 2/36 (5%)
 Frame = +1

Query: 89  FLFSFVLIQYLQYISCNCWVQD--TFDIDQTEHNKE 122
           +LFS  + +YL  + CN W  D  TF  + T H+ E
Sbjct: 637 YLFSGEVDEYLDLVDCNSWGGDENTFTTNNTHHHDE 744


>TC78447 similar to GP|7768151|emb|CAB90633.1 protein phpsphatase 2C (PP2C)
           {Fagus sylvatica}, partial (37%)
          Length = 833

 Score = 29.3 bits (64), Expect = 1.4
 Identities = 11/27 (40%), Positives = 22/27 (80%)
 Frame = +2

Query: 76  LSQVFVNIII*FIFLFSFVLIQYLQYI 102
           + Q+ ++++I FIF FS +LIQ+L+++
Sbjct: 23  IKQIKISLLIIFIFQFS*ILIQWLKFV 103


>TC88552 similar to PIR|T51260|T51260 hypothetical protein T8M16_50 -
           Arabidopsis thaliana, partial (25%)
          Length = 1588

 Score = 28.9 bits (63), Expect = 1.9
 Identities = 22/101 (21%), Positives = 47/101 (45%), Gaps = 9/101 (8%)
 Frame = +2

Query: 126 NTAKRLYG-SHRGKLHEHFKKYE--TNEMALEHKPDEISEE------DWEHLVDKFSSPT 176
           ++++  YG S   K  + ++K+E  ++  A + + D++ EE      D+   +DK+S   
Sbjct: 377 HSSRTSYGHSRHDKYADEYRKHERLSSRSARDSRGDQMREESDSRSKDYGRSMDKYSREK 556

Query: 177 YKEMSARNKANKSKQVINHRCGRKSFQAVSYDARDPETQKE 217
           Y+    R+K    ++   +    K  +    DARD +   +
Sbjct: 557 YERSDFRSKDRDREKRATYEEVEKEKRTRDGDARDEKKDSQ 679


>BQ144022 homologue to GP|22831315|dbj hypothetical protein~predicted by
           GlimmerM etc. {Oryza sativa (japonica cultivar-group)},
           partial (16%)
          Length = 1286

 Score = 28.1 bits (61), Expect = 3.2
 Identities = 15/44 (34%), Positives = 22/44 (49%)
 Frame = +2

Query: 147 ETNEMALEHKPDEISEEDWEHLVDKFSSPTYKEMSARNKANKSK 190
           ET +  L H P   S    +H   K S PT++  +ARNK  + +
Sbjct: 47  ETTQRFLGHTPQNFSSLKRKH---KTSKPTHERRTARNKKQRGQ 169


>TC88202 homologue to GP|21615421|emb|CAD33930. arabinogalactan protein
           {Cicer arietinum}, complete
          Length = 628

 Score = 27.7 bits (60), Expect = 4.2
 Identities = 12/21 (57%), Positives = 16/21 (76%)
 Frame = +2

Query: 85  I*FIFLFSFVLIQYLQYISCN 105
           I*F + FSF+LI+Y  Y+S N
Sbjct: 524 I*FFYYFSFMLIKYYFYLSIN 586


>TC84372 similar to GP|15146181|gb|AAK83574.1 AT3g14070/MAG2_2 {Arabidopsis
           thaliana}, partial (35%)
          Length = 755

 Score = 27.3 bits (59), Expect = 5.5
 Identities = 14/54 (25%), Positives = 25/54 (45%)
 Frame = -1

Query: 30  IPLDKMVAVGPGAPDFVTEISVLVKKKAPFNVKGWKKVPDTAKDHILSQVFVNI 83
           I  D + +V   + D    +     K APF ++  K +PD+A  H++     N+
Sbjct: 662 IHXDAVTSVRKSSTDAAENVGT---KSAPFMIEVIKLLPDSANSHVIQAAGANV 510


>CA990302 weakly similar to GP|10177640|db contains similarity to
           heparanase~gene_id:MGG23.2 {Arabidopsis thaliana},
           partial (42%)
          Length = 786

 Score = 26.9 bits (58), Expect = 7.2
 Identities = 9/14 (64%), Positives = 11/14 (78%)
 Frame = +1

Query: 52  LVKKKAPFNVKGWK 65
           + KK+ PFN KGWK
Sbjct: 559 ITKKRVPFNSKGWK 600


>TC86794 similar to PIR|T06499|T06499 Rieske [2Fe-2S] iron-sulfur protein
           tic55 - garden pea, partial (11%)
          Length = 812

 Score = 26.9 bits (58), Expect = 7.2
 Identities = 16/74 (21%), Positives = 32/74 (42%), Gaps = 5/74 (6%)
 Frame = -2

Query: 142 HFKKYETNEMALEHKPDEISEEDWEHLVDKFSSPTYKEMSARNKANKSKQVI-----NHR 196
           HF K+   + +     D IS  ++EH+    S  + + M  + +   ++++I       R
Sbjct: 436 HFSKFGKYKPSSSRSSDGISPSEYEHMRTAISKMSAENMELKERLKTNEELIRASQEESR 257

Query: 197 CGRKSFQAVSYDAR 210
             R+  Q    D+R
Sbjct: 256 LAREQAQHSQEDSR 215


>BG449891 similar to GP|17901818|gb 32 kDa ferritin subunit {Galleria
           mellonella}, partial (74%)
          Length = 649

 Score = 26.9 bits (58), Expect = 7.2
 Identities = 18/78 (23%), Positives = 35/78 (44%), Gaps = 2/78 (2%)
 Frame = +1

Query: 142 HFKKYETNEMALEHKPDEISEEDWEHLVDKFSSPTYK--EMSARNKANKSKQVINHRCGR 199
           +F  Y+TN +       ++S+E WE  +D     T +  EM+   ++    +  N+    
Sbjct: 358 YFNNYQTNRLGFSKLFKKLSDEAWEKTIDIIKHVTMRGDEMNFDQRSTMKVERKNYTVEL 537

Query: 200 KSFQAVSYDARDPETQKE 217
              +A+   A+  +TQ E
Sbjct: 538 HELEAL---AKALDTQXE 582


>TC85947 similar to PIR|S52261|S52261 NADH dehydrogenase (ubiquinone) (EC
           1.6.5.3) flavoprotein 1 precursor - potato, partial
           (44%)
          Length = 1086

 Score = 26.6 bits (57), Expect = 9.4
 Identities = 12/36 (33%), Positives = 20/36 (55%)
 Frame = -2

Query: 9   TTGTSVTKKKMKSATGKLPLCIPLDKMVAVGPGAPD 44
           +T T+   + + S   +LPLCI  +  + +GP  PD
Sbjct: 698 STVTTPWSRPLTSGLQQLPLCILFNPSLKLGPEMPD 591


>TC86147 GP|9758956|dbj|BAB09343.1 histone H2A {Arabidopsis thaliana},
           partial (94%)
          Length = 678

 Score = 26.6 bits (57), Expect = 9.4
 Identities = 19/56 (33%), Positives = 27/56 (47%), Gaps = 10/56 (17%)
 Frame = +1

Query: 4   AGRGKTTGTSVTKKKMKSATGKLPLCIPLDKMV----------AVGPGAPDFVTEI 49
           AGRGKT G S + KK  S + K  L  P+ ++            VG GAP ++  +
Sbjct: 103 AGRGKTLG-SGSAKKATSRSSKAGLQFPVGRIARFLKAGKYAERVGAGAPVYLAAV 267


>TC92979 similar to GP|20127014|gb|AAM10934.1 putative bHLH transcription
           factor {Arabidopsis thaliana}, partial (15%)
          Length = 744

 Score = 26.6 bits (57), Expect = 9.4
 Identities = 9/22 (40%), Positives = 14/22 (62%)
 Frame = +3

Query: 55  KKAPFNVKGWKKVPDTAKDHIL 76
           +K  + +K WK+ P  AK H+L
Sbjct: 441 QKGHWKIKSWKQKPQKAKGHVL 506


  Database: MTGI
    Posted date:  Oct 22, 2004  3:39 PM
  Number of letters in database: 27,044,181
  Number of sequences in database:  36,976
  
Lambda     K      H
   0.318    0.133    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,831,861
Number of Sequences: 36976
Number of extensions: 113147
Number of successful extensions: 717
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 713
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 717
length of query: 243
length of database: 9,014,727
effective HSP length: 93
effective length of query: 150
effective length of database: 5,575,959
effective search space: 836393850
effective search space used: 836393850
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)


Lotus: description of TM0302a.10